MD15G1293100.v1.1

DnaJ domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003816
Physical Location & Seq
Forward (+)
27167738 .. 27171827
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1293100.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGCTTCTCATAGCGCCAAAGACGACGTTCTGCTTCGCCGTCGTCCAATTCTTCTGCTATTACTTCAACGGAACCCAAGGCGCAATCGGAGTGGTGGAGCTCAGTGGTTCAGTAGCAGAACCGAGTCAGCCCGGACCGAGTTCGGCTCAGAAAACGCGTACGAGGTCCTAGGTGTTTCCGAAACCAGCTCATTTGCCGAAATCAAAGCGTCTTTCCACAAATTGGCCAAGCAAACTCACCCGGACCTCGCCGAGTCGAAGACCGATTCCGATGCTTCTCGCCGATTCGTGCAAATCCTCGCCGCCTATGAGATTCTTTCGGATTGCGAAAAGAGGGCCCATTACGACATGGACCTGTTATCTCAGAGAAAGCTTGTGCAGAAACATTCTGAGCATGTCATGACATTCAGCATGTATAAGGGTCATCTAAGTACTTATAAGAAGATGGAAGTTGTTGAATGGTTGAAGTGGTATAGACTAGCTATAAATGATGTTTTGTCGGAGAGGAGGGTGGTGGTTGGTACAGGTTATTTTGATGTTCTTGAGAGAGATTTCTATTCAGCCATACATGCGGCTTACTATGGCCCTCTGATAGAGTCTATGGAGCTTCTTCCGGACTGCTTCGAAGCTGAAGAGAGGTCTGTGTATGAAACTCCAGAGGTGCTGCATTTGGTATCGGGGCGTGACCTTTTTGGGATGGTCCGGCTGGGCAATAAAATTCCAGAATTGTCATATTACAACAATATGAAATTGAATTATTCCACATCTGCGGGTTTGGGAATTTGTCGTTCTGTTGAGGACTTTAGCATCAGCAAGAACTCTGGTGGCATTGATGATGTTGGAAACGCAAATACAGAAACCTGGGATATCAAAAATGACCCACCAGATGCATATAAGGATTTAGAATTGCATCTATGTGGAAGGGTGGTTGCTACAGCCACCAGAGTCCCTCCAAAAAACAACCGTGATGGGAAACAGAATGAAGAGTCTGATCGCATTCATGTTTTTCTCAGCTGTAATGAAAATCCTTTGCATACCAACAAAGGGTTTTCTCAAGATTCATTTTTAGGTGGTGCCGTTGGATCAAGGATTCTGTTGGGTACAATAACAGGATTAGGGACTAGCTCTGAAGAAGGGTCCTGCTTTGTCTACAATAGCAGTGGTTCCAAAACCCATGTGATTATGAAGCATAGAACATTGCTGGTGAAACACTTGTATTGGTATGAATTGGGAGAAAAGGTTTCTGTTTGTGAGTGCAGATGCAGTAGAGCTCGGTTGCCACCAAGCAAATTTTGGCTGTTTGAGCCTCGCTGTGGCATGCATGACATAGGCGGTTGGTATATTGAAACATTTGGTAGAGATAAGAAAGGCCGCATGTTGCCATCTCAACGGTATTGGGATGGCTTTGATGCTGGAAAACAATTTGAAAAGAGACTCCATCCTGCGATGTATCTTCTTTCTCTTGCATATAGAACTTTAGATCTTGAAGATGCACAAAGAAGGAAAAATACATTCAAGGATGTCGTGGACAGACGACTTTTTAAAATTCTCAATTGGTGCAAAAGACTTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

525

Amino Acids

60.03

Weight (kDa)

8.58

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 52 - 116 1.4e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 1073
AccB7I CCANNNNNTGG 1 cut(s) 223
AccI GTMKAC 1 cut(s) 1149
AccII CGCG 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 613
AciI CCGC 5 cut(s) 303, 572, 770, 1332, 1372
AclWI GGATC 1 cut(s) 1090
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 4 cut(s) 717, 780, 1289, 1545
AcuI CTGAAG 2 cut(s) 651, 1149
AfaI GTAC 4 cut(s) 161, 433, 523, 1102
AfiI CCNNNNNNNGG 3 cut(s) 223, 1044, 1313
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 8 cut(s) 68, 458, 466, 754, 1346, 1427, 1487, 1516
AjnI CCWGG 1 cut(s) 860
AluBI AGCT 9 cut(s) 101, 189, 373, 482, 607, 629, 1014, 1125, 1271
AluI AGCT 9 cut(s) 101, 189, 373, 482, 607, 629, 1014, 1125, 1271
Alw21I GWGCWC 2 cut(s) 103, 1273
Alw26I GTCTC 1 cut(s) 1426
AlwI GGATC 1 cut(s) 1090
Aor13HI TCCGGA 1 cut(s) 613
AoxI GGCC 4 cut(s) 225, 336, 583, 1369
ApaI GGGCCC 1 cut(s) 340
ApeKI GCWGC 1 cut(s) 664
ApoI RAATTY 4 cut(s) 717, 780, 1289, 1545
AspA2I CCTAGG 1 cut(s) 169
AspLEI GCGC 2 cut(s) 17, 84
AspS9I GGNCC 9 cut(s) 135, 166, 244, 336, 337, 352, 584, 700, 1137
AsuC2I CCSGG 2 cut(s) 133, 242
AsuHPI GGTGA 2 cut(s) 230, 1216
AsuII TTCGAA 1 cut(s) 624
AvaII GGWCC 6 cut(s) 135, 166, 244, 352, 700, 1137
AvrII CCTAGG 1 cut(s) 169
BaeGI GKGCMC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 1 cut(s) 1073
BanII GRGCYC 3 cut(s) 103, 340, 1273
BarI GAAGNNNNNNTAC 2 cut(s) 1492, 1524
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 2 cut(s) 103, 1273
BbvI GCAGC 1 cut(s) 651
BccI CCATC 6 cut(s) 439, 691, 962, 1390, 1394, 1446
BceAI ACGGC 2 cut(s) 24, 1061
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 862
BcnI CCSGG 2 cut(s) 133, 242
BcoDI GTCTC 1 cut(s) 1426
BfaI CTAG 3 cut(s) 170, 479, 1122
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 18
BglII AGATCT 1 cut(s) 1480
BisI GCNGC 4 cut(s) 303, 573, 665, 1372
BlnI CCTAGG 1 cut(s) 169
BlsI GCNGC 4 cut(s) 304, 574, 666, 1373
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 3 cut(s) 133, 242, 862
Bme18I GGWCC 6 cut(s) 135, 166, 244, 352, 700, 1137
BmgT120I GGNCC 9 cut(s) 135, 166, 244, 336, 337, 352, 584, 700, 1137
BmiI GGNNCC 5 cut(s) 74, 338, 1075, 1138, 1165
BmrFI CCNGG 3 cut(s) 133, 242, 862
BmsI GCATC 7 cut(s) 262, 816, 877, 919, 1250, 1399, 1480
BpiI GAAGAC 1 cut(s) 266
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 624
BpuEI CTTGAG 2 cut(s) 563, 1038
BpuMI CCSGG 2 cut(s) 133, 242
BsaJI CCNNGG 3 cut(s) 77, 169, 861
BsaWI WCCGGW 1 cut(s) 613
BsaXI ACNNNNNCTCC 2 cut(s) 1224, 1254
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 1044, 1313
Bse3DI GCAATG 1 cut(s) 1196
BseAI TCCGGA 1 cut(s) 613
BseBI CCWGG 1 cut(s) 862
BseDI CCNNGG 3 cut(s) 77, 169, 861
BseGI GGATG 4 cut(s) 702, 1405, 1438, 1525
BseLI CCNNNNNNNGG 3 cut(s) 223, 1044, 1313
BseMI GCAATG 1 cut(s) 1196
BseMII CTCAG 5 cut(s) 116, 162, 377, 381, 1024
BseRI GAGGAG 1 cut(s) 520
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 1 cut(s) 651
BseYI CCCAGC 1 cut(s) 706
BsgI GTGCAG 2 cut(s) 398, 1276
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 4 cut(s) 227, 338, 585, 1371
BshNI GGYRCC 1 cut(s) 1073
BsiHKAI GWGCWC 2 cut(s) 103, 1273
BsiSI CCGG 4 cut(s) 133, 242, 614, 703
BsiWI CGTACG 1 cut(s) 159
BslFI GGGAC 2 cut(s) 932, 1132
BslI CCNNNNNNNGG 3 cut(s) 223, 1044, 1313
BsmAI GTCTC 1 cut(s) 1426
BsmFI GGGAC 2 cut(s) 932, 1132
BsmI GAATGC 1 cut(s) 996
BsnI GGCC 4 cut(s) 227, 338, 585, 1371
Bsp119I TTCGAA 1 cut(s) 624
Bsp120I GGGCCC 1 cut(s) 336
Bsp1286I GDGCHC 3 cut(s) 103, 340, 1273
Bsp13I TCCGGA 1 cut(s) 613
Bsp143I GATC 3 cut(s) 991, 1082, 1480
BspACI CCGC 5 cut(s) 303, 572, 770, 1332, 1372
BspANI GGCC 4 cut(s) 227, 338, 585, 1371
BspCNI CTCAG 5 cut(s) 115, 161, 376, 382, 1023
BspEI TCCGGA 1 cut(s) 613
BspFNI CGCG 1 cut(s) 158
BspHI TCATGA 1 cut(s) 399
BspLI GGNNCC 5 cut(s) 74, 338, 1075, 1138, 1165
BspPI GGATC 1 cut(s) 1090
BspT104I TTCGAA 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 1073
BsrDI GCAATG 1 cut(s) 1196
BssECI CCNNGG 3 cut(s) 77, 169, 861
BssMI GATC 3 cut(s) 991, 1082, 1480
BssT1I CCWWGG 2 cut(s) 77, 169
Bst2UI CCWGG 1 cut(s) 862
Bst4CI ACNGT 2 cut(s) 965, 1392
Bst6I CTCTTC 2 cut(s) 627, 978
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 1 cut(s) 1319
BstDEI CTNAG 6 cut(s) 102, 148, 363, 390, 428, 1010
BstF5I GGATG 4 cut(s) 702, 1405, 1438, 1525
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 2 cut(s) 17, 84
BstKTI GATC 3 cut(s) 994, 1085, 1483
BstMAI GTCTC 1 cut(s) 1426
BstMBI GATC 3 cut(s) 991, 1082, 1480
BstMWI GCNNNNNNNGC 2 cut(s) 569, 1303
BstNI CCWGG 1 cut(s) 862
BstNSI RCATGY 5 cut(s) 398, 415, 572, 1321, 1378
BstSCI CCNGG 3 cut(s) 131, 240, 860
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 1 cut(s) 340
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 651
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 1480
BstYI RGATCY 1 cut(s) 1480
BsuRI GGCC 4 cut(s) 227, 338, 585, 1371
BtgZI GCGATG 1 cut(s) 1460
BtsCI GGATG 4 cut(s) 702, 1405, 1438, 1525
BtsI GCAGTG 1 cut(s) 1165
BtsIMutI CAGTG 2 cut(s) 110, 1165
Cac8I GCNNGC 1 cut(s) 1319
CciI TCATGA 1 cut(s) 399
CfoI GCGC 2 cut(s) 17, 84
Cfr13I GGNCC 9 cut(s) 135, 166, 244, 336, 337, 352, 584, 700, 1137
CpoI CGGWCCG 1 cut(s) 135
CseI GACGC 1 cut(s) 198
Csp6I GTAC 4 cut(s) 160, 432, 522, 1101
CspCI CAANNNNNGTGG 4 cut(s) 73, 108, 1141, 1176
CspI CGGWCCG 1 cut(s) 135
CviQI GTAC 4 cut(s) 160, 432, 522, 1101
DdeI CTNAG 6 cut(s) 102, 148, 363, 390, 428, 1010
DpnI GATC 3 cut(s) 993, 1084, 1482
DpnII GATC 3 cut(s) 991, 1082, 1480
DraI TTTAAA 1 cut(s) 1543
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 627, 978
EarI CTCTTC 2 cut(s) 627, 978
Ecl136II GAGCTC 2 cut(s) 101, 1271
Eco130I CCWWGG 2 cut(s) 77, 169
Eco24I GRGCYC 3 cut(s) 103, 340, 1273
Eco32I GATATC 1 cut(s) 868
Eco47I GGWCC 6 cut(s) 135, 166, 244, 352, 700, 1137
Eco53kI GAGCTC 2 cut(s) 101, 1271
Eco57I CTGAAG 2 cut(s) 651, 1149
EcoICRI GAGCTC 2 cut(s) 101, 1271
EcoO109I RGGNCCY 3 cut(s) 166, 336, 1137
EcoRII CCWGG 1 cut(s) 860
EcoRV GATATC 1 cut(s) 868
EcoT14I CCWWGG 2 cut(s) 77, 169
EcoT22I ATGCAT 2 cut(s) 892, 1323
EcoT38I GRGCYC 3 cut(s) 103, 340, 1273
ErhI CCWWGG 2 cut(s) 77, 169
FaqI GGGAC 2 cut(s) 932, 1132
FauI CCCGC 1 cut(s) 763
FblI GTMKAC 1 cut(s) 1149
Fnu4HI GCNGC 4 cut(s) 303, 573, 665, 1372
FokI GGATG 4 cut(s) 709, 1412, 1425, 1532
FriOI GRGCYC 3 cut(s) 103, 340, 1273
Fsp4HI GCNGC 4 cut(s) 303, 573, 665, 1372
FspBI CTAG 3 cut(s) 170, 479, 1122
GlaI GCGC 2 cut(s) 16, 83
GluI GCNGC 4 cut(s) 303, 573, 665, 1372
GsaI CCCAGC 1 cut(s) 710
GsuI CTGGAG 1 cut(s) 639
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 4 cut(s) 227, 338, 585, 1371
HapII CCGG 4 cut(s) 133, 242, 614, 703
HgaI GACGC 1 cut(s) 198
HhaI GCGC 2 cut(s) 17, 84
Hin6I GCGC 2 cut(s) 15, 82
HinP1I GCGC 2 cut(s) 15, 82
HindIII AAGCTT 1 cut(s) 371
HpaII CCGG 4 cut(s) 133, 242, 614, 703
HphI GGTGA 2 cut(s) 230, 1216
Hpy166II GTNNAC 2 cut(s) 1150, 1528
Hpy188III TCNNGA 7 cut(s) 400, 542, 614, 656, 722, 1055, 1484
Hpy8I GTNNAC 2 cut(s) 1150, 1528
Hpy99I CGWCG 2 cut(s) 29, 45
HpyAV CCTTC 3 cut(s) 915, 1127, 1494
HpyCH4III ACNGT 2 cut(s) 965, 1392
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 2 cut(s) 569, 1303
HpyF3I CTNAG 6 cut(s) 102, 148, 363, 390, 428, 1010
HpySE526I ACGT 1 cut(s) 27
HspAI GCGC 2 cut(s) 15, 82
Kpn2I TCCGGA 1 cut(s) 613
Kzo9I GATC 3 cut(s) 991, 1082, 1480
LmnI GCTCC 2 cut(s) 98, 604
Lsp1109I GCAGC 1 cut(s) 651
LweI GCATC 7 cut(s) 262, 816, 877, 919, 1250, 1399, 1480
MaeI CTAG 3 cut(s) 170, 479, 1122
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 683
MalI GATC 3 cut(s) 993, 1084, 1482
MboI GATC 3 cut(s) 991, 1082, 1480
MboII GAAGA 9 cut(s) 44, 271, 454, 602, 644, 995, 1142, 1445, 1499
MfeI CAATTG 1 cut(s) 1552
MflI RGATCY 1 cut(s) 1480
MhlI GDGCHC 3 cut(s) 103, 340, 1273
MlsI TGGCCA 1 cut(s) 227
MluI ACGCGT 1 cut(s) 156
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 6 cut(s) 134, 263, 605, 954, 995, 1428
MmeI TCCRAC 3 cut(s) 480, 820, 1060
Mox20I TGGCCA 1 cut(s) 227
Mph1103I ATGCAT 2 cut(s) 892, 1323
MroI TCCGGA 1 cut(s) 613
MscI TGGCCA 1 cut(s) 227
MseI TTAA 1 cut(s) 1542
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 1014
MspI CCGG 4 cut(s) 133, 242, 614, 703
MspR9I CCNGG 3 cut(s) 133, 242, 862
MunI CAATTG 1 cut(s) 1552
Mva1269I GAATGC 1 cut(s) 996
MvaI CCWGG 1 cut(s) 862
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 2 cut(s) 569, 1303
NciI CCSGG 2 cut(s) 133, 242
NdeII GATC 3 cut(s) 991, 1082, 1480
NlaIV GGNNCC 5 cut(s) 74, 338, 1075, 1138, 1165
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 683
NsiI ATGCAT 2 cut(s) 892, 1323
NspI RCATGY 5 cut(s) 398, 415, 572, 1321, 1378
NspV TTCGAA 1 cut(s) 624
PaeI GCATGC 1 cut(s) 1321
PagI TCATGA 1 cut(s) 399
PctI GAATGC 1 cut(s) 996
PfeI GAWTC 5 cut(s) 266, 285, 313, 1058, 1090
Pfl23II CGTACG 1 cut(s) 159
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 4 cut(s) 304, 574, 666, 1373
PleI GAGTC 6 cut(s) 133, 262, 604, 953, 994, 1428
PpsI GAGTC 6 cut(s) 133, 262, 604, 953, 994, 1428
PpuMI RGGWCCY 2 cut(s) 166, 1137
PsiI TTATAA 1 cut(s) 438
Psp124BI GAGCTC 2 cut(s) 103, 1273
Psp5II RGGWCCY 2 cut(s) 166, 1137
Psp6I CCWGG 1 cut(s) 860
PspFI CCCAGC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 860
PspLI CGTACG 1 cut(s) 159
PspN4I GGNNCC 5 cut(s) 74, 338, 1075, 1138, 1165
PspOMI GGGCCC 1 cut(s) 336
PspPI GGNCC 9 cut(s) 135, 166, 244, 336, 337, 352, 584, 700, 1137
PspPPI RGGWCCY 2 cut(s) 166, 1137
PsuI RGATCY 1 cut(s) 1480
PvuII CAGCTG 1 cut(s) 1014
RsaI GTAC 4 cut(s) 161, 433, 523, 1102
RsaNI GTAC 4 cut(s) 160, 432, 522, 1101
RseI CAYNNNNRTG 1 cut(s) 915
Rsr2I CGGWCCG 1 cut(s) 135
RsrII CGGWCCG 1 cut(s) 135
SacI GAGCTC 2 cut(s) 103, 1273
SaqAI TTAA 1 cut(s) 1542
SatI GCNGC 4 cut(s) 303, 573, 665, 1372
Sau3AI GATC 3 cut(s) 991, 1082, 1480
Sau96I GGNCC 9 cut(s) 135, 166, 244, 336, 337, 352, 584, 700, 1137
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 6 cut(s) 134, 263, 605, 954, 995, 1428
ScrFI CCNGG 3 cut(s) 133, 242, 862
SduI GDGCHC 3 cut(s) 103, 340, 1273
SfaNI GCATC 7 cut(s) 262, 816, 877, 919, 1250, 1399, 1480
SfcI CTRYAG 1 cut(s) 933
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 6 cut(s) 135, 166, 244, 352, 700, 1137
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 2 cut(s) 542, 1053
SmoI CTYRAG 2 cut(s) 542, 1053
SphI GCATGC 1 cut(s) 1321
SsiI CCGC 5 cut(s) 303, 572, 770, 1332, 1372
SspMI CTAG 3 cut(s) 170, 479, 1122
SstI GAGCTC 2 cut(s) 103, 1273
StyD4I CCNGG 3 cut(s) 131, 240, 860
StyI CCWWGG 2 cut(s) 77, 169
TaaI ACNGT 2 cut(s) 965, 1392
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 257, 624
TaqII GACCGA 2 cut(s) 152, 278
TatI WGTACW 1 cut(s) 431
TauI GCSGC 3 cut(s) 305, 575, 1374
TfiI GAWTC 5 cut(s) 266, 285, 313, 1058, 1090
Tru1I TTAA 1 cut(s) 1542
Tru9I TTAA 1 cut(s) 1542
TscAI CASTG 2 cut(s) 110, 1165
TseFI GTSAC 1 cut(s) 683
TseI GCWGC 1 cut(s) 664
Tsp45I GTSAC 1 cut(s) 683
TspDTI ATGAA 8 cut(s) 663, 761, 989, 996, 1035, 1050, 1199, 1239
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 2 cut(s) 110, 1165
Van91I CCANNNNNTGG 1 cut(s) 223
VpaK11BI GGWCC 6 cut(s) 135, 166, 244, 352, 700, 1137
XapI RAATTY 4 cut(s) 717, 780, 1289, 1545
XceI RCATGY 5 cut(s) 398, 415, 572, 1321, 1378
XcmI CCANNNNNNNNNTGG 1 cut(s) 1290
XmaJI CCTAGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 1149
XspI CTAG 3 cut(s) 170, 479, 1122
ZrmI AGTACT 1 cut(s) 433
Zsp2I ATGCAT 2 cut(s) 892, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.