Rroxscaffold_2G00136420

DnaJ domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003816
Physical Location & Seq
Reverse (-)
74120420 .. 74123981
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136420.1

Sequence Viewer

Length: 1536 bp
ATGCCTTCTCTCTCCTGCGACCAGTCTCTCTATCGCCGCCGTCCAATTCAACCGTTTCCAAGGCGTTATCTCTTACTATTCTATTACCGGACTGCCGGAACTCGGTGGTTCAGCACCAGCACAGCCGAGTCAACTCGGACCGAGTTCACGGAAGAGAACGCCTACGAGCTCTTAGGCGTTTCGGAGACGAGCTCATTCGCCGAAATCAAAGCGTCGTTTCATAAATTGGCGAAACAGACTCACCCGGACCTCGTTCAGTCCGGTAACGATTCCGACGTTTCTCGCCGATTCGTTCAAATCCTTGCCGCCTATGAGGGACCTGTTATCTCAGCGAAAGCTTATGCAGAAGCAATCGGGGGAGGTGGTGCGTTGCACTTGTATAAGTTTCATAGTACATATAGGGGGATGGAAGTTGTTGAGTGGCTGAAATGGTATAGAATTGCTATAAGTGATGTGTTGGCGGAGAGGAAGGTGGTTGTTGGAACAGGTTATTTTGATGGGCTTGAGAGAGATTTTTATTCAGCTATACATGCGGCGTATTATGGTCCTGATATTGAGTCTATCGACTTTCTTCCTGACTGCTTTGAAGCTGAAGAGAGGTCTGTGTATGAAACTCCGGAGGTGCTGCATTTGGTTTCGGGGCGTGATGTTTTTGGGATGGTCTGCTTAGTAGATAAAGTTCCAGAGCTGCCATATTATAGCAATAAGAGGTTAAAGTATTCCACATCAGCGGGTTTAGGTATTTGCCAATCTGGTGAGGATTTGAGAATCTCTATGAGCTCGGGTGGTGTTTATAATGATGGATATGCCCATATGCAAGCTGCGGATGTCTCAACCCACACATCTGATACTTATAGAGATTTAGAATTGCATATATCTGGAAGGGTGGTTGCTACAGCCACAAGAGTCCCTCCCAAAAGCTATTGTGACCGGATACAGAATGAAGACACCGATCACATTCATGTTTTTCTGAGCTCTGATGATAATCCTGTGCATACCAGCAAAGGGTTTACTGAAGCTCCCTCTTCAGGCAGTGCTGTGGGATCAAGGATTCTATTGGGAACTATAACTGGATTGCGGTCTAGCTCAGAAGAAGGGTCTTGCCTTGTCTACAACAGCAGTGGTACCAAAACCCATGTGATTATGAAGCATAGAACACTTCTGGTGAAACACATGCATTGGTATGGATTGGGAGACAATGTTTCTGTATGTGAGTGTAGATGCACAAGAGCACGATTACCACCAAGCAAATTTTGGTTGTTTGAGCCTCGTTGTGGCATGCATGACATAGGCGGTTGGTATGTTGAAACATTTGGCAAAGATAAGAAAGGACGGTTATCACCATCACAGAGATATTGGGATGGCTTTGATGCAAGGGAACAATCTGAGAAGAGACTCCATCCTGCGATGTACCTTCTTGCTATGGGATATAGAACTTTAGATCTTGAAGATGCAAAAAGAAGGAAACATACATTCAGGGATTTTGTCGACAGAAAGCTCTTTAAAATACTCAATTTGTGCAAAAGACTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

511

Amino Acids

57.89

Weight (kDa)

7.62

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 53 - 105 5e-11 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
Acc65I GGTACC 1 cut(s) 1124
AccB1I GGYRCC 1 cut(s) 1124
AccI GTMKAC 2 cut(s) 1110, 1488
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 8 cut(s) 37, 306, 461, 533, 731, 824, 1078, 1293
AclWI GGATC 1 cut(s) 1051
AcsI RAATTY 1 cut(s) 1250
AcuI CTGAAG 3 cut(s) 612, 1011, 1035
AfaI GTAC 3 cut(s) 394, 1126, 1412
AfiI CCNNNNNNNGG 4 cut(s) 102, 1005, 1028, 1274
AgsI TTSAA 5 cut(s) 50, 296, 587, 1307, 1448
Alw21I GWGCWC 5 cut(s) 171, 194, 782, 977, 1234
Alw26I GTCTC 5 cut(s) 30, 179, 835, 1188, 1387
AlwI GGATC 1 cut(s) 1051
Ama87I CYCGRG 1 cut(s) 781
Aor13HI TCCGGA 1 cut(s) 616
ApeKI GCWGC 3 cut(s) 625, 688, 821
ApoI RAATTY 1 cut(s) 1250
Asp718I GGTACC 1 cut(s) 1124
AspS9I GGNCC 4 cut(s) 138, 247, 317, 545
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 4 cut(s) 233, 767, 1177, 1332
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 4 cut(s) 138, 247, 317, 545
BanI GGYRCC 1 cut(s) 1124
BanII GRGCYC 4 cut(s) 171, 194, 782, 977
BarI GAAGNNNNNNTAC 2 cut(s) 1453, 1485
BbsI GAAGAC 1 cut(s) 951
Bbv12I GWGCWC 5 cut(s) 171, 194, 782, 977, 1234
BbvI GCAGC 3 cut(s) 612, 675, 808
BccI CCATC 7 cut(s) 400, 491, 652, 794, 1351, 1355, 1407
BceAI ACGGC 1 cut(s) 24
BciVI GTATCC 1 cut(s) 927
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 5 cut(s) 30, 179, 835, 1188, 1387
BfaI CTAG 1 cut(s) 1083
BfmI CTRYAG 1 cut(s) 894
BfuI GTATCC 1 cut(s) 927
BglII AGATCT 1 cut(s) 1441
BisI GCNGC 6 cut(s) 37, 306, 534, 626, 689, 822
BlsI GCNGC 6 cut(s) 38, 307, 535, 627, 690, 823
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 4 cut(s) 138, 247, 317, 545
BmeT110I CYCGRG 1 cut(s) 781
BmgT120I GGNCC 4 cut(s) 138, 247, 317, 545
BmiI GGNNCC 2 cut(s) 318, 1126
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 1211, 1360, 1441
BpiI GAAGAC 1 cut(s) 951
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 984
BsaJI CCNNGG 1 cut(s) 59
BsaWI WCCGGW 4 cut(s) 87, 260, 616, 930
BsaXI ACNNNNNCTCC 4 cut(s) 1003, 1033, 1185, 1215
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 1005, 1028, 1274
Bse1I ACTGG 2 cut(s) 22, 1075
Bse8I GATNNNNATC 1 cut(s) 984
BseAI TCCGGA 1 cut(s) 616
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 5 cut(s) 411, 663, 832, 1366, 1399
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 4 cut(s) 102, 1005, 1028, 1274
BseMII CTCAG 4 cut(s) 342, 962, 1101, 1377
BseNI ACTGG 2 cut(s) 22, 1075
BseXI GCAGC 3 cut(s) 612, 675, 808
BshNI GGYRCC 1 cut(s) 1124
BsiHKAI GWGCWC 5 cut(s) 171, 194, 782, 977, 1234
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 6 cut(s) 88, 96, 245, 261, 617, 931
BslFI GGGAC 2 cut(s) 330, 893
BslI CCNNNNNNNGG 4 cut(s) 102, 1005, 1028, 1274
BsmAI GTCTC 5 cut(s) 30, 179, 835, 1188, 1387
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 330, 893
BsoBI CYCGRG 1 cut(s) 781
Bsp1286I GDGCHC 5 cut(s) 171, 194, 782, 977, 1234
Bsp13I TCCGGA 1 cut(s) 616
Bsp143I GATC 3 cut(s) 952, 1043, 1441
BspACI CCGC 8 cut(s) 37, 306, 461, 533, 731, 824, 1078, 1293
BspCNI CTCAG 4 cut(s) 341, 963, 1100, 1378
BspEI TCCGGA 1 cut(s) 616
BspLI GGNNCC 2 cut(s) 318, 1126
BspPI GGATC 1 cut(s) 1051
BspT107I GGYRCC 1 cut(s) 1124
BsrI ACTGG 2 cut(s) 22, 1075
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 3 cut(s) 952, 1043, 1441
BssT1I CCWWGG 1 cut(s) 59
Bst4CI ACNGT 2 cut(s) 54, 1335
Bst6I CTCTTC 4 cut(s) 147, 588, 1030, 1385
BstC8I GCNNGC 2 cut(s) 819, 1280
BstDEI CTNAG 6 cut(s) 172, 328, 667, 971, 1087, 1386
BstF5I GGATG 5 cut(s) 411, 663, 832, 1366, 1399
BstKTI GATC 3 cut(s) 955, 1046, 1444
BstMAI GTCTC 5 cut(s) 30, 179, 835, 1188, 1387
BstMBI GATC 3 cut(s) 952, 1043, 1441
BstMWI GCNNNNNNNGC 1 cut(s) 530
BstNSI RCATGY 3 cut(s) 533, 1177, 1282
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 894
BstV1I GCAGC 3 cut(s) 612, 675, 808
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 1 cut(s) 1441
BstYI RGATCY 1 cut(s) 1441
BsuI GTATCC 1 cut(s) 927
BtgZI GCGATG 1 cut(s) 1421
BtsCI GGATG 5 cut(s) 411, 663, 832, 1366, 1399
BtsI GCAGTG 2 cut(s) 1039, 1126
BtsIMutI CAGTG 2 cut(s) 1039, 1126
Cac8I GCNNGC 2 cut(s) 819, 1280
Cfr13I GGNCC 4 cut(s) 138, 247, 317, 545
CpoI CGGWCCG 1 cut(s) 138
CseI GACGC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 393, 1125, 1411
CspCI CAANNNNNGTGG 2 cut(s) 1102, 1137
CspI CGGWCCG 1 cut(s) 138
CviAII CATG 6 cut(s) 530, 962, 1136, 1174, 1279, 1283
CviQI GTAC 3 cut(s) 393, 1125, 1411
DdeI CTNAG 6 cut(s) 172, 328, 667, 971, 1087, 1386
DpnI GATC 3 cut(s) 954, 1045, 1443
DpnII GATC 3 cut(s) 952, 1043, 1441
DraI TTTAAA 1 cut(s) 1504
Eam1104I CTCTTC 4 cut(s) 147, 588, 1030, 1385
EarI CTCTTC 4 cut(s) 147, 588, 1030, 1385
EciI GGCGGA 1 cut(s) 476
Ecl136II GAGCTC 4 cut(s) 169, 192, 780, 975
Eco130I CCWWGG 1 cut(s) 59
Eco24I GRGCYC 4 cut(s) 171, 194, 782, 977
Eco47I GGWCC 4 cut(s) 138, 247, 317, 545
Eco53kI GAGCTC 4 cut(s) 169, 192, 780, 975
Eco57I CTGAAG 3 cut(s) 612, 1011, 1035
Eco88I CYCGRG 1 cut(s) 781
EcoICRI GAGCTC 4 cut(s) 169, 192, 780, 975
EcoO109I RGGNCCY 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 59
EcoT22I ATGCAT 2 cut(s) 1179, 1284
EcoT38I GRGCYC 4 cut(s) 171, 194, 782, 977
ErhI CCWWGG 1 cut(s) 59
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 6 cut(s) 533, 965, 1139, 1177, 1282, 1286
FaqI GGGAC 2 cut(s) 330, 893
FatI CATG 6 cut(s) 529, 961, 1135, 1173, 1278, 1282
FauI CCCGC 1 cut(s) 724
FauNDI CATATG 1 cut(s) 813
FblI GTMKAC 2 cut(s) 1110, 1488
Fnu4HI GCNGC 6 cut(s) 37, 306, 534, 626, 689, 822
FokI GGATG 5 cut(s) 418, 670, 839, 1373, 1386
FriOI GRGCYC 4 cut(s) 171, 194, 782, 977
Fsp4HI GCNGC 6 cut(s) 37, 306, 534, 626, 689, 822
FspBI CTAG 1 cut(s) 1083
GluI GCNGC 6 cut(s) 37, 306, 534, 626, 689, 822
HapII CCGG 6 cut(s) 88, 96, 245, 261, 617, 931
HgaI GACGC 1 cut(s) 201
Hin1II CATG 6 cut(s) 533, 965, 1139, 1177, 1282, 1286
HincII GTYRAC 2 cut(s) 132, 1489
HindII GTYRAC 2 cut(s) 132, 1489
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 9 cut(s) 128, 238, 269, 288, 557, 768, 906, 1051, 1395
HpaII CCGG 6 cut(s) 88, 96, 245, 261, 617, 931
HphI GGTGA 4 cut(s) 233, 767, 1177, 1332
Hpy166II GTNNAC 5 cut(s) 132, 147, 1011, 1111, 1489
Hpy188I TCNGA 8 cut(s) 138, 184, 274, 847, 972, 979, 1090, 1387
Hpy188III TCNNGA 6 cut(s) 548, 575, 617, 683, 879, 1445
Hpy8I GTNNAC 5 cut(s) 132, 147, 1011, 1111, 1489
Hpy99I CGWCG 2 cut(s) 217, 278
HpyAV CCTTC 6 cut(s) 15, 463, 876, 1088, 1424, 1455
HpyCH4III ACNGT 2 cut(s) 54, 1335
HpyCH4IV ACGT 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 530
HpyF3I CTNAG 6 cut(s) 172, 328, 667, 971, 1087, 1386
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 6 cut(s) 533, 965, 1139, 1177, 1282, 1286
Kpn2I TCCGGA 1 cut(s) 616
KpnI GGTACC 1 cut(s) 1128
Kzo9I GATC 3 cut(s) 952, 1043, 1441
LmnI GCTCC 1 cut(s) 1024
Lsp1109I GCAGC 3 cut(s) 612, 675, 808
LweI GCATC 3 cut(s) 1211, 1360, 1441
MaeI CTAG 1 cut(s) 1083
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 263, 926
MalI GATC 3 cut(s) 954, 1045, 1443
MboI GATC 3 cut(s) 952, 1043, 1441
MboII GAAGA 8 cut(s) 164, 563, 605, 956, 1017, 1103, 1402, 1460
MflI RGATCY 1 cut(s) 1441
MhlI GDGCHC 5 cut(s) 171, 194, 782, 977, 1234
MluCI AATT 6 cut(s) 45, 224, 438, 866, 1250, 1513
MlyI GAGTC 5 cut(s) 137, 232, 566, 915, 1389
MmeI TCCRAC 2 cut(s) 297, 460
Mph1103I ATGCAT 2 cut(s) 1179, 1284
MroI TCCGGA 1 cut(s) 616
MseI TTAA 3 cut(s) 713, 1503, 1534
MslI CAYNNNNRTG 2 cut(s) 960, 1182
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 6 cut(s) 88, 96, 245, 261, 617, 931
MspR9I CCNGG 1 cut(s) 245
MwoI GCNNNNNNNGC 1 cut(s) 530
NciI CCSGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 813
NdeII GATC 3 cut(s) 952, 1043, 1441
NlaIII CATG 6 cut(s) 533, 965, 1139, 1177, 1282, 1286
NlaIV GGNNCC 2 cut(s) 318, 1126
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 926
NsiI ATGCAT 2 cut(s) 1179, 1284
NspI RCATGY 3 cut(s) 533, 1177, 1282
PaeI GCATGC 1 cut(s) 1282
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 4 cut(s) 269, 288, 768, 1051
PkrI GCNGC 6 cut(s) 38, 307, 535, 627, 690, 823
PleI GAGTC 5 cut(s) 136, 232, 565, 914, 1389
PpsI GAGTC 5 cut(s) 136, 232, 565, 914, 1389
PpuMI RGGWCCY 1 cut(s) 317
PsiI TTATAA 1 cut(s) 795
Psp124BI GAGCTC 4 cut(s) 171, 194, 782, 977
Psp5II RGGWCCY 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 318, 1126
PspPI GGNCC 4 cut(s) 138, 247, 317, 545
PspPPI RGGWCCY 1 cut(s) 317
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PsuI RGATCY 1 cut(s) 1441
RsaI GTAC 3 cut(s) 394, 1126, 1412
RsaNI GTAC 3 cut(s) 393, 1125, 1411
RseI CAYNNNNRTG 2 cut(s) 960, 1182
Rsr2I CGGWCCG 1 cut(s) 138
RsrII CGGWCCG 1 cut(s) 138
SacI GAGCTC 4 cut(s) 171, 194, 782, 977
SalI GTCGAC 1 cut(s) 1487
SaqAI TTAA 3 cut(s) 713, 1503, 1534
SatI GCNGC 6 cut(s) 37, 306, 534, 626, 689, 822
Sau3AI GATC 3 cut(s) 952, 1043, 1441
Sau96I GGNCC 4 cut(s) 138, 247, 317, 545
SchI GAGTC 5 cut(s) 137, 232, 566, 915, 1389
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 5 cut(s) 171, 194, 782, 977, 1234
SfaNI GCATC 3 cut(s) 1211, 1360, 1441
SfcI CTRYAG 1 cut(s) 894
SinI GGWCC 4 cut(s) 138, 247, 317, 545
SmiMI CAYNNNNRTG 2 cut(s) 960, 1182
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SphI GCATGC 1 cut(s) 1282
Sse9I AATT 6 cut(s) 45, 224, 438, 866, 1250, 1513
SsiI CCGC 8 cut(s) 37, 306, 461, 533, 731, 824, 1078, 1293
SspMI CTAG 1 cut(s) 1083
SstI GAGCTC 4 cut(s) 171, 194, 782, 977
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 54, 1335
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 564, 1488
TaqII GACCGA 1 cut(s) 155
TasI AATT 6 cut(s) 45, 224, 438, 866, 1250, 1513
TatI WGTACW 1 cut(s) 392
TauI GCSGC 3 cut(s) 39, 308, 536
TfiI GAWTC 4 cut(s) 269, 288, 768, 1051
Tru1I TTAA 3 cut(s) 713, 1503, 1534
Tru9I TTAA 3 cut(s) 713, 1503, 1534
TscAI CASTG 2 cut(s) 1039, 1126
TseFI GTSAC 1 cut(s) 926
TseI GCWGC 3 cut(s) 625, 688, 821
Tsp45I GTSAC 1 cut(s) 926
TspDTI ATGAA 6 cut(s) 209, 377, 624, 950, 957, 1160
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 2 cut(s) 1039, 1126
VpaK11BI GGWCC 4 cut(s) 138, 247, 317, 545
XapI RAATTY 1 cut(s) 1250
XceI RCATGY 3 cut(s) 533, 1177, 1282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1251
XmiI GTMKAC 2 cut(s) 1110, 1488
XspI CTAG 1 cut(s) 1083
Zsp2I ATGCAT 2 cut(s) 1179, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.