Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0003816

Physical Location & Seq

Genomic Coordinates Forward (+)

12517583 .. 12521040

Gene Structure 3458 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0142300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2460 bp

ATGTTGAAGATATCATTGATGAATTCATGTATCGCATGTATGAGAAGGAAAGTGGCGGTCGATTTGCAAGCAATCAAAGCCATTCCAGAGAGAAAACAGAGGTATGGTCTAATTGGTCAAGAAGGAACATCTTCTGATGATGTTCACAAGAGGGTAAAAATCAAAGCCGAGTCCTCGCTTTTTGAGAAGGAAGATGAACTCGTGGGAATTGAAGGGAAGAAGCAAATGTTAATGGGATGGTTGATCAATGGAGAGAAAAAACAAACTATTGTCTCTGTGGTCGGCATGGGAGGATCAGGGAAGACAACTCTCGTTGCCAACACCTTCACTCAGGAGACAGTAAAGAGACATTTTAATTGTTTTGCATGGATAACTGTTTCCCAAACTTATGTGCTTGAAGACTTATTTAGAAGCTTGATAAAGGAATTCCACCAATCAATAAAACAGGAAGTCCCTGGGAATTTGCATGCAATGAGCTACAGGGAATTGCTGGAGATGTTGACAAATTTTTTGGTGTCCAAAAGATATCTAGTTGTATTGGATGACGTGTGGGATATCACTCTTTGGGAAAATATAAGATTATCATTTCCAGATGAACAGATTGGAAGTCGAATCATTCTTACAACTCGAAGAGAAGACATAGCATCCTATTCTTTTGGAGTTGAAAGTCATGTTTACCCTATTCAACTCTTGCAACGCGATGAGGCCATGGAGCTCTTCAGCAAGAAAGCATTCCCAACTTATCACAATATATGCCCGCCAGAGCTTGAACCATTAGCTTGGGAACTTGTGGAGAAGTGCAACGGCTTACCTCTGGCAATTGTGGCTTTACGTGGCCTCATGTCCTCAAAAAAGTCTTCTGTAGAATGGAATACAGTCCGCAACAGCTTAAATTGGCATCTAAGTAACAATCCGTTGTTAGAATCTATAAAGATCATCTTGTTGCTTAGTTTCAACGGTTTGTCATATCGATTAAAACATTGTTTCCTATATTGTTCCTTATTCCCAGAGGATTACTTTATAGGGAAAAATAGACTCATTAGACTGTGGATAGCTGAAGGATTTATCGAACACGTTAAAGGGGTTACACCAGAAGCAGTTGCAGAACTCTATATTATGGAACTCGTTTCTCGTAGCATGCTACAAGCTGTAAGAAGAAATGATTATGAAACCGGAAGGCCAAGGGCATGCAAGATGCATGATCTTATGCGGGAGCTTGCTTTATCGGAATCAGAATCGGAGAACTTTGCTACTGTGTGCGATGGCAAAGAAGTAATGAAAGAAATGGGAGCCCGTCGTTTGTCAATTCAAACAACCGATGGGGAAATTACGCCACTCACAGATATGTCGCATCTTCGTTCTTTTCTTGTCTTTGTCACTAATAGAATCTCATCGTCTGTTTCTGAGATATTGCCTTCTGGATTGAAGTTGTTGAGGATTCTAGATTTGGAAAGGTCCCCGGTTACTAAATTGCCAGGTGAAATTGTGTACTTATTCAACTTAAGATTTTTAAATTTGAGGAAAACTCCCATTAAAGAGCTTCCCAAATCCATAGGACGGCTCCACAATCTCCAGACCTTGGACATAAGGGATTCCAATATAAAGGCACTTCCAAGGGGAATTACCAAGCTGCTTAACCTACGCCATCTGATTATGTACCATTACACTGGAGTACATAAGGGCTTCAGATATATTGAAGGGACAAAATGTCCTTCTGGTATTTGTAAGTTGAAGAAGTTGCAAGTTTTGGCATGTGTTGAATCAGAAGGAAATGTTATTAGACTTGTCGGGAACATGACCCAACTTACTAAGCTTGGCATCACAAATGTAAAAGAAAGAGACGAGATAGACCTATGTGCTTCAATTCAAAAGATGGAGCTCCTCCAGGACTTGTTTTTAATGGTACCTGATGAAGAGGAATGTCTTCGACTCGATGCGCTAACTGCACCTCCTGCTCACCTTCACACACTTGCGTTGGCTGGAAAGTTGGAAATGGTACCACGTTGGTTTTCTTCACTTCACAGCCTCACATCTTTGAATCTGCATTGGTCTAGACTTGAGGAAGATTTATTACCTCACATTGAAGCACTGGCCAGCCTACGAAGCCTTACTCTTTATAATGCATACACTGGGAAAAACCTTAGTTTCAGTACAGGCTTCAGAAATCTTGTGCATTTGCAGCTGTGCAATTTCCCTTTACTGAATAACATAACTATAGAGAAAGGGGTGATGCCAAAGCTGCAGTTCTTGATGCTGGATAGCTGTCTGGAAGTAAAGACTTTGCCGTGGGGTATTGAATACCTTTCTAATCTTGAAACCTTGCAGTTGGGATCTGTTTCAGTGCAACTTCTAGAGTCCATACGAGAAGGAGGTGTGGATCATTCAAAGGTGCGACACCTTCCAGAAGTCGATGATTTAGTCAGAACGTTATTTGGGAGGTATAATGACAGCAAGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1230

Protein Analysis

819

Amino Acids

93.37

Weight (kDa)

8.31

Isoelectric Point (pI)

50.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NB-ARC	PF00931	72 - 247	4.5e-45	NB-ARC domain
WHD_DRP	PF23559	334 - 407	1e-22	Disease resistance protein Winged helix domain
LRR_14	PF23598	451 - 775	2.2e-43	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003816)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37480
fragaria_vesca	FvH4_1g17190 FvH4_1g17190
malus_domestica	MD15G1293100.v1.1
prunus_persica	Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1 Prupe.6G224500_v2.0.a1
pyrus_communis	pycom15g25610
rosa_chinensis	RchiOBHm_Chr2g0107341
rosa_laevigata	RLG00000017571
rosa_multiflora	Rmu_sc0010952.1_g000014
rosa_roxburghii	Rroxscaffold_2G00136420
rosa_rugosa	Rorug02G0142200 Rorug02G0142300
rosa_samantha	Rh2AG192900 Rh2BG205000 Rh2CG198200 Rh2DG200200
rosa_wichuraiana	Rw2G015280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2118
Acc65I	GGTACC	2 cut(s)	1903, 1996
AccB1I	GGYRCC	2 cut(s)	1903, 1996
AccB7I	CCANNNNNTGG	1 cut(s)	1579
AccII	CGCG	1 cut(s)	699
AciI	CCGC	4 cut(s)	56, 758, 880, 1210
AclI	AACGTT	1 cut(s)	2426
AclWI	GGATC	3 cut(s)	301, 2338, 2385
AcoI	YGGCCR	1 cut(s)	2091
AcsI	RAATTY	5 cut(s)	22, 425, 460, 505, 1513
AcuI	CTGAAG	4 cut(s)	703, 1077, 1669, 2143
AfaI	GTAC	6 cut(s)	1490, 1658, 1674, 1905, 1998, 2152
AfiI	CCNNNNNNNGG	2 cut(s)	1557, 1579
AflII	CTTAAG	1 cut(s)	1501
AflIII	ACRYGT	2 cut(s)	546, 1072
AhdI	GACNNNNNGTC	2 cut(s)	1707, 2452
AjiI	CACGTC	1 cut(s)	547
AjnI	CCWGG	3 cut(s)	454, 1474, 1884
Alw21I	GWGCWC	2 cut(s)	717, 1881
Alw26I	GTCTC	4 cut(s)	277, 329, 340, 1833
AlwI	GGATC	3 cut(s)	301, 2338, 2385
AoxI	GGCC	4 cut(s)	705, 835, 1178, 2091
ApeKI	GCWGC	3 cut(s)	1630, 2179, 2239
ApoI	RAATTY	5 cut(s)	22, 425, 460, 505, 1513
ArsI	GACNNNNNNTTYG	2 cut(s)	493, 525
Asp700I	GAANNNNTTC	3 cut(s)	130, 731, 1923
Asp718I	GGTACC	2 cut(s)	1903, 1996
AspLEI	GCGC	1 cut(s)	1939
AspS9I	GGNCC	1 cut(s)	1455
AsuC2I	CCSGG	1 cut(s)	1460
AsuHPI	GGTGA	3 cut(s)	1490, 1949, 2239
AvaII	GGWCC	1 cut(s)	1455
BalI	TGGCCA	1 cut(s)	2093
BanI	GGYRCC	2 cut(s)	1903, 1996
BanII	GRGCYC	3 cut(s)	717, 1294, 1881
BarI	GAAGNNNNNNTAC	2 cut(s)	2055, 2087
BauI	CACGAG	1 cut(s)	200
BbsI	GAAGAC	5 cut(s)	308, 405, 642, 849, 1916
Bbv12I	GWGCWC	2 cut(s)	717, 1881
BbvI	GCAGC	3 cut(s)	1617, 2191, 2226
BccI	CCATC	5 cut(s)	231, 1256, 1313, 1653, 1867
BceAI	ACGGC	3 cut(s)	820, 1574, 2269
BciT130I	CCWGG	3 cut(s)	456, 1476, 1886
BclI	TGATCA	1 cut(s)	243
BcnI	CCSGG	1 cut(s)	1460
BcoDI	GTCTC	4 cut(s)	277, 329, 340, 1833
BfaI	CTAG	5 cut(s)	530, 1442, 2052, 2351, 2458
BfmI	CTRYAG	4 cut(s)	478, 861, 2214, 2240
BfrI	CTTAAG	1 cut(s)	1501
BisI	GCNGC	3 cut(s)	1631, 2180, 2240
BlsI	GCNGC	3 cut(s)	1632, 2181, 2241
Bme1390I	CCNGG	4 cut(s)	456, 1460, 1476, 1886
Bme18I	GGWCC	1 cut(s)	1455
BmeRI	GACNNNNNGTC	2 cut(s)	1707, 2452
BmgBI	CACGTC	1 cut(s)	547
BmgT120I	GGNCC	1 cut(s)	1455
BmiI	GGNNCC	5 cut(s)	1291, 1457, 1562, 1905, 1998
BmrFI	CCNGG	4 cut(s)	456, 1460, 1476, 1886
BmrI	ACTGGG	1 cut(s)	2139
BmsI	GCATC	8 cut(s)	653, 907, 1185, 1360, 1827, 1924, 2220, 2241
BmuI	ACTGGG	1 cut(s)	2139
BpiI	GAAGAC	5 cut(s)	308, 405, 642, 849, 1916
BplI	GAGNNNNNCTC	2 cut(s)	1510, 1542
BpmI	CTGGAG	4 cut(s)	512, 1556, 1689, 1868
BpuEI	CTTGAG	1 cut(s)	2078
BpuMI	CCSGG	1 cut(s)	1460
Bsa29I	ATCGAT	1 cut(s)	970
BsaAI	YACGTR	1 cut(s)	833
BsaJI	CCNNGG	8 cut(s)	454, 455, 708, 1181, 1458, 1578, 1613, 2285
BsaWI	WCCGGW	1 cut(s)	1172
BsaXI	ACNNNNNCTCC	2 cut(s)	1933, 1963
Bsc4I	CCNNNNNNNGG	2 cut(s)	1557, 1579
Bse1I	ACTGG	3 cut(s)	1672, 2094, 2134
Bse3DI	GCAATG	1 cut(s)	477
BseBI	CCWGG	3 cut(s)	456, 1476, 1886
BseCI	ATCGAT	1 cut(s)	970
BseDI	CCNNGG	8 cut(s)	454, 455, 708, 1181, 1458, 1578, 1613, 2285
BseGI	GGATG	3 cut(s)	242, 547, 644
BseLI	CCNNNNNNNGG	2 cut(s)	1557, 1579
BseMI	GCAATG	1 cut(s)	477
BseMII	CTCAG	2 cut(s)	344, 1395
BseNI	ACTGG	3 cut(s)	1672, 2094, 2134
BseRI	GAGGAG	1 cut(s)	1871
BseXI	GCAGC	3 cut(s)	1617, 2191, 2226
BsgI	GTGCAG	1 cut(s)	1929
Bsh1236I	CGCG	1 cut(s)	699
Bsh1285I	CGRYCG	1 cut(s)	60
BshFI	GGCC	4 cut(s)	707, 837, 1180, 2093
BshNI	GGYRCC	2 cut(s)	1903, 1996
BshVI	ATCGAT	1 cut(s)	970
BsiEI	CGRYCG	1 cut(s)	60
BsiHKAI	GWGCWC	2 cut(s)	717, 1881
BsiSI	CCGG	2 cut(s)	1173, 1460
BslFI	GGGAC	3 cut(s)	437, 1441, 1714
BslI	CCNNNNNNNGG	2 cut(s)	1557, 1579
BsmAI	GTCTC	4 cut(s)	277, 329, 340, 1833
BsmBI	CGTCTC	1 cut(s)	1833
BsmFI	GGGAC	3 cut(s)	437, 1441, 1714
BsmI	GAATGC	1 cut(s)	731
BsnI	GGCC	4 cut(s)	707, 837, 1180, 2093
Bsp1286I	GDGCHC	3 cut(s)	717, 1294, 1881
Bsp143I	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
Bsp19I	CCATGG	1 cut(s)	708
BspACI	CCGC	4 cut(s)	56, 758, 880, 1210
BspANI	GGCC	4 cut(s)	707, 837, 1180, 2093
BspCNI	CTCAG	2 cut(s)	343, 1396
BspDI	ATCGAT	1 cut(s)	970
BspFNI	CGCG	1 cut(s)	699
BspLI	GGNNCC	5 cut(s)	1291, 1457, 1562, 1905, 1998
BspMAI	CTGCAG	1 cut(s)	2244
BspPI	GGATC	3 cut(s)	301, 2338, 2385
BspQI	GCTCTTC	1 cut(s)	722
BspT107I	GGYRCC	2 cut(s)	1903, 1996
BspTI	CTTAAG	1 cut(s)	1501
BsrDI	GCAATG	1 cut(s)	477
BsrI	ACTGG	3 cut(s)	1672, 2094, 2134
BssECI	CCNNGG	8 cut(s)	454, 455, 708, 1181, 1458, 1578, 1613, 2285
BssMI	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
BssSI	CACGAG	1 cut(s)	200
BssT1I	CCWWGG	4 cut(s)	708, 1181, 1578, 1613
Bst2BI	CACGAG	1 cut(s)	200
Bst2UI	CCWGG	3 cut(s)	456, 1476, 1886
Bst4CI	ACNGT	6 cut(s)	340, 376, 877, 959, 1047, 1255
Bst6I	CTCTTC	3 cut(s)	625, 722, 1908
BstAFI	CTTAAG	1 cut(s)	1501
BstAPI	GCANNNNNTGC	1 cut(s)	1952
BstBAI	YACGTR	1 cut(s)	833
BstC8I	GCNNGC	7 cut(s)	69, 468, 758, 1139, 1189, 1218, 2095
BstDEI	CTNAG	6 cut(s)	330, 902, 947, 1404, 1809, 2141
BstDSI	CCRYGG	2 cut(s)	708, 2285
BstF5I	GGATG	3 cut(s)	242, 547, 644
BstFNI	CGCG	1 cut(s)	699
BstHHI	GCGC	1 cut(s)	1939
BstKTI	GATC	6 cut(s)	246, 296, 936, 1204, 2333, 2380
BstMAI	GTCTC	4 cut(s)	277, 329, 340, 1833
BstMBI	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
BstMCI	CGRYCG	1 cut(s)	60
BstMWI	GCNNNNNNNGC	7 cut(s)	77, 824, 1943, 1952, 2103, 2179, 2239
BstNI	CCWGG	3 cut(s)	456, 1476, 1886
BstNSI	RCATGY	5 cut(s)	39, 470, 1141, 1191, 1755
BstSCI	CCNGG	4 cut(s)	454, 1458, 1474, 1884
BstSFI	CTRYAG	4 cut(s)	478, 861, 2214, 2240
BstUI	CGCG	1 cut(s)	699
BstV1I	GCAGC	3 cut(s)	1617, 2191, 2226
BstV2I	GAAGAC	5 cut(s)	308, 405, 642, 849, 1916
BstX2I	RGATCY	1 cut(s)	2330
BstXI	CCANNNNNNTGG	2 cut(s)	780, 1667
BstYI	RGATCY	1 cut(s)	2330
Bsu15I	ATCGAT	1 cut(s)	970
BsuRI	GGCC	4 cut(s)	707, 837, 1180, 2093
BsuTUI	ATCGAT	1 cut(s)	970
BtgI	CCRYGG	2 cut(s)	708, 2285
BtgZI	GCGATG	2 cut(s)	714, 1275
BtrI	CACGTC	1 cut(s)	547
BtsCI	GGATG	3 cut(s)	242, 547, 644
BtsIMutI	CAGTG	4 cut(s)	1665, 2087, 2127, 2346
Cac8I	GCNNGC	7 cut(s)	69, 468, 758, 1139, 1189, 1218, 2095
CfoI	GCGC	1 cut(s)	1939
Cfr13I	GGNCC	1 cut(s)	1455
ClaI	ATCGAT	1 cut(s)	970
Csp6I	GTAC	6 cut(s)	1489, 1657, 1673, 1904, 1997, 2151
CviQI	GTAC	6 cut(s)	1489, 1657, 1673, 1904, 1997, 2151
DdeI	CTNAG	6 cut(s)	330, 902, 947, 1404, 1809, 2141
DpnI	GATC	6 cut(s)	245, 295, 935, 1203, 2332, 2379
DpnII	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
DraI	TTTAAA	1 cut(s)	1512
DriI	GACNNNNNGTC	2 cut(s)	1707, 2452
EaeI	YGGCCR	1 cut(s)	2091
Eam1104I	CTCTTC	3 cut(s)	625, 722, 1908
Eam1105I	GACNNNNNGTC	2 cut(s)	1707, 2452
EarI	CTCTTC	3 cut(s)	625, 722, 1908
Ecl136II	GAGCTC	2 cut(s)	715, 1879
Eco130I	CCWWGG	4 cut(s)	708, 1181, 1578, 1613
Eco24I	GRGCYC	3 cut(s)	717, 1294, 1881
Eco32I	GATATC	3 cut(s)	12, 527, 556
Eco47I	GGWCC	1 cut(s)	1455
Eco53kI	GAGCTC	2 cut(s)	715, 1879
Eco57I	CTGAAG	4 cut(s)	703, 1077, 1669, 2143
EcoICRI	GAGCTC	2 cut(s)	715, 1879
EcoO109I	RGGNCCY	1 cut(s)	1455
EcoRI	GAATTC	2 cut(s)	22, 425
EcoRII	CCWGG	3 cut(s)	454, 1474, 1884
EcoRV	GATATC	3 cut(s)	12, 527, 556
EcoT14I	CCWWGG	4 cut(s)	708, 1181, 1578, 1613
EcoT22I	ATGCAT	2 cut(s)	1200, 2125
EcoT38I	GRGCYC	3 cut(s)	717, 1294, 1881
ErhI	CCWWGG	4 cut(s)	708, 1181, 1578, 1613
Esp3I	CGTCTC	1 cut(s)	1833
FalI	AAGNNNNNCTT	2 cut(s)	923, 955
FaqI	GGGAC	3 cut(s)	437, 1441, 1714
FauI	CCCGC	2 cut(s)	765, 1203
FbaI	TGATCA	1 cut(s)	243
Fnu4HI	GCNGC	3 cut(s)	1631, 2180, 2240
FokI	GGATG	3 cut(s)	249, 554, 631
FriOI	GRGCYC	3 cut(s)	717, 1294, 1881
Fsp4HI	GCNGC	3 cut(s)	1631, 2180, 2240
FspBI	CTAG	5 cut(s)	530, 1442, 2052, 2351, 2458
GlaI	GCGC	1 cut(s)	1938
GluI	GCNGC	3 cut(s)	1631, 2180, 2240
GsuI	CTGGAG	4 cut(s)	512, 1556, 1689, 1868
HaeIII	GGCC	4 cut(s)	707, 837, 1180, 2093
HapII	CCGG	2 cut(s)	1173, 1460
HhaI	GCGC	1 cut(s)	1939
Hin6I	GCGC	1 cut(s)	1937
HinP1I	GCGC	1 cut(s)	1937
HincII	GTYRAC	1 cut(s)	501
HindII	GTYRAC	1 cut(s)	501
HindIII	AAGCTT	2 cut(s)	412, 1811
HpaII	CCGG	2 cut(s)	1173, 1460
HphI	GGTGA	3 cut(s)	1490, 1949, 2239
Hpy166II	GTNNAC	4 cut(s)	145, 501, 676, 1489
Hpy8I	GTNNAC	4 cut(s)	145, 501, 676, 1489
Hpy99I	CGWCG	1 cut(s)	1299
HpyCH4III	ACNGT	6 cut(s)	340, 376, 877, 959, 1047, 1255
HpyCH4IV	ACGT	5 cut(s)	546, 832, 1074, 2002, 2426
HpyF10VI	GCNNNNNNNGC	7 cut(s)	77, 824, 1943, 1952, 2103, 2179, 2239
HpyF3I	CTNAG	6 cut(s)	330, 902, 947, 1404, 1809, 2141
HpySE526I	ACGT	5 cut(s)	546, 832, 1074, 2002, 2426
HspAI	GCGC	1 cut(s)	1937
KpnI	GGTACC	2 cut(s)	1907, 2000
Ksp22I	TGATCA	1 cut(s)	243
Kzo9I	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
LguI	GCTCTTC	1 cut(s)	722
LmnI	GCTCC	6 cut(s)	712, 1213, 1289, 1566, 1876, 1884
Lsp1109I	GCAGC	3 cut(s)	1617, 2191, 2226
LweI	GCATC	8 cut(s)	653, 907, 1185, 1360, 1827, 1924, 2220, 2241
MaeI	CTAG	5 cut(s)	530, 1442, 2052, 2351, 2458
MaeII	ACGT	5 cut(s)	546, 832, 1074, 2002, 2426
MaeIII	GTNAC	4 cut(s)	905, 1084, 1375, 1462
MalI	GATC	6 cut(s)	245, 295, 935, 1203, 2332, 2379
MboI	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
MfeI	CAATTG	1 cut(s)	819
MflI	RGATCY	1 cut(s)	2330
MhlI	GDGCHC	3 cut(s)	717, 1294, 1881
MlsI	TGGCCA	1 cut(s)	2093
MluNI	TGGCCA	1 cut(s)	2093
MlyI	GAGTC	4 cut(s)	179, 1029, 1923, 2363
MmeI	TCCRAC	1 cut(s)	1968
Mox20I	TGGCCA	1 cut(s)	2093
Mph1103I	ATGCAT	2 cut(s)	1200, 2125
MroXI	GAANNNNTTC	3 cut(s)	130, 731, 1923
MscI	TGGCCA	1 cut(s)	2093
MslI	CAYNNNNRTG	1 cut(s)	1343
Msp20I	TGGCCA	1 cut(s)	2093
MspA1I	CMGCKG	1 cut(s)	2182
MspCI	CTTAAG	1 cut(s)	1501
MspI	CCGG	2 cut(s)	1173, 1460
MspR9I	CCNGG	4 cut(s)	456, 1460, 1476, 1886
MunI	CAATTG	1 cut(s)	819
Mva1269I	GAATGC	1 cut(s)	731
MvaI	CCWGG	3 cut(s)	456, 1476, 1886
MvnI	CGCG	1 cut(s)	699
MwoI	GCNNNNNNNGC	7 cut(s)	77, 824, 1943, 1952, 2103, 2179, 2239
NciI	CCSGG	1 cut(s)	1460
NcoI	CCATGG	1 cut(s)	708
NdeII	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
NlaIV	GGNNCC	5 cut(s)	1291, 1457, 1562, 1905, 1998
NmeAIII	GCCGAG	1 cut(s)	193
NmuCI	GTSAC	1 cut(s)	1375
NsiI	ATGCAT	2 cut(s)	1200, 2125
NspI	RCATGY	5 cut(s)	39, 470, 1141, 1191, 1755
PaeI	GCATGC	3 cut(s)	470, 1141, 1191
PasI	CCCWGGG	1 cut(s)	455
PciSI	GCTCTTC	1 cut(s)	722
PctI	GAATGC	1 cut(s)	731
PdmI	GAANNNNTTC	3 cut(s)	130, 731, 1923
PfeI	GAWTC	9 cut(s)	612, 923, 1229, 1235, 1386, 1438, 1592, 1760, 2038
PflMI	CCANNNNNTGG	1 cut(s)	1579
PfoI	TCCNGGA	1 cut(s)	1884
PkrI	GCNGC	3 cut(s)	1632, 2181, 2241
PleI	GAGTC	4 cut(s)	178, 1029, 1923, 2362
PpsI	GAGTC	4 cut(s)	178, 1029, 1923, 2362
Ppu21I	YACGTR	1 cut(s)	833
PpuMI	RGGWCCY	1 cut(s)	1455
PsiI	TTATAA	1 cut(s)	2118
Psp124BI	GAGCTC	2 cut(s)	717, 1881
Psp1406I	AACGTT	1 cut(s)	2426
Psp5II	RGGWCCY	1 cut(s)	1455
Psp6I	CCWGG	3 cut(s)	454, 1474, 1884
PspGI	CCWGG	3 cut(s)	454, 1474, 1884
PspN4I	GGNNCC	5 cut(s)	1291, 1457, 1562, 1905, 1998
PspPI	GGNCC	1 cut(s)	1455
PspPPI	RGGWCCY	1 cut(s)	1455
PstI	CTGCAG	1 cut(s)	2244
PsuI	RGATCY	1 cut(s)	2330
PvuII	CAGCTG	1 cut(s)	2182
RsaI	GTAC	6 cut(s)	1490, 1658, 1674, 1905, 1998, 2152
RsaNI	GTAC	6 cut(s)	1489, 1657, 1673, 1904, 1997, 2151
RseI	CAYNNNNRTG	1 cut(s)	1343
SacI	GAGCTC	2 cut(s)	717, 1881
SapI	GCTCTTC	1 cut(s)	722
SatI	GCNGC	3 cut(s)	1631, 2180, 2240
Sau3AI	GATC	6 cut(s)	243, 293, 933, 1201, 2330, 2377
Sau96I	GGNCC	1 cut(s)	1455
SchI	GAGTC	4 cut(s)	179, 1029, 1923, 2363
ScrFI	CCNGG	4 cut(s)	456, 1460, 1476, 1886
SduI	GDGCHC	3 cut(s)	717, 1294, 1881
SfaNI	GCATC	8 cut(s)	653, 907, 1185, 1360, 1827, 1924, 2220, 2241
SfcI	CTRYAG	4 cut(s)	478, 861, 2214, 2240
SinI	GGWCC	1 cut(s)	1455
SmiMI	CAYNNNNRTG	1 cut(s)	1343
SmlI	CTYRAG	2 cut(s)	1501, 2057
SmoI	CTYRAG	2 cut(s)	1501, 2057
SphI	GCATGC	3 cut(s)	470, 1141, 1191
SsiI	CCGC	4 cut(s)	56, 758, 880, 1210
SspMI	CTAG	5 cut(s)	530, 1442, 2052, 2351, 2458
SstI	GAGCTC	2 cut(s)	717, 1881
StyD4I	CCNGG	4 cut(s)	454, 1458, 1474, 1884
StyI	CCWWGG	4 cut(s)	708, 1181, 1578, 1613
TaaI	ACNGT	6 cut(s)	340, 376, 877, 959, 1047, 1255
TaiI	ACGT	5 cut(s)	549, 835, 1077, 2005, 2429
TaqI	TCGA	8 cut(s)	60, 610, 628, 970, 1068, 1927, 1932, 2409
TatI	WGTACW	3 cut(s)	1488, 1672, 2150
TfiI	GAWTC	9 cut(s)	612, 923, 1229, 1235, 1386, 1438, 1592, 1760, 2038
TscAI	CASTG	4 cut(s)	1672, 2094, 2134, 2346
TseFI	GTSAC	1 cut(s)	1375
TseI	GCWGC	3 cut(s)	1630, 2179, 2239
Tsp45I	GTSAC	1 cut(s)	1375
TspDTI	ATGAA	7 cut(s)	15, 35, 210, 609, 1182, 1292, 1926
TspGWI	ACGGA	1 cut(s)	903
TspRI	CASTG	4 cut(s)	1672, 2094, 2134, 2346
Van91I	CCANNNNNTGG	1 cut(s)	1579
Vha464I	CTTAAG	1 cut(s)	1501
VpaK11BI	GGWCC	1 cut(s)	1455
XapI	RAATTY	5 cut(s)	22, 425, 460, 505, 1513
XbaI	TCTAGA	3 cut(s)	1441, 2051, 2350
XceI	RCATGY	5 cut(s)	39, 470, 1141, 1191, 1755
XmnI	GAANNNNTTC	3 cut(s)	130, 731, 1923
XspI	CTAG	5 cut(s)	530, 1442, 2052, 2351, 2458
Zsp2I	ATGCAT	2 cut(s)	1200, 2125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.