Rh2BG205000

DnaJ domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003816
Physical Location & Seq
Forward (+)
18972589 .. 18975351
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG205000.1

Sequence Viewer

Length: 1575 bp
ATGCCTTCTCTCTCCTGCGACCAGTCTCTCTATCGCCGCCGTCCAATTCAACTGTTTCCAAGGCGTTATCTCTTACTATTCTATTACCGGACCGCCGGAACTCGGTGGTTCAGCGCCAGCACCGCCGAGTCAACTCGGACCGAGTTTACGGAAGAGAACGCCTACGAGCTCTTAGGCGTTTCGGAGACGAGCTCATTTGCCGAAATCAAAGCGTCGTTTCATAAATTGGCGAAACAGACTCACCCGGACCTCGTTCAGTCCGGTAACGATTCCGACGTTTCTCTCCGATTCGTTCAAATCCTCGCCGCCTATGAGATTCTTTCGGATTGCGAAAGGAGGGCGCATTATGACAGGGACCTGTTATCTCAGCGAAAGCTTATGCAGAAGCAATCCGGGGGAGGTGGTGCATTGCACTTGTATAAGTTTCATAGTACATATAGGGGGATGGAAGTTGTTGAGTGGCTGAAATGGTATAGAATTGCTATAAGTGATGTGTTGGCGGAGAGGAAGGTGGTTGTTGGAACAGGTTATTTTGATGGGCTTGAGAGAGATTTTTATTCAGCTATACATGCGGCGTTTTATGGTCCTGATATTGGGTCTATCGACTTTCTTCCCGACTGCTTTGAAGCTGAAGAGAGGTCTGTGTATGAAACTCCGGAGGTGCTGCATTTGGTTTCGGGCCGTGATGTTTTTGGGATGGTCTGCTTAGTAGATAAAATTCCAGAGCTGCCATATTATAGCAATAAGAGGTTAAAGTATTCCACATCAGCGGGTTTAGGTATTTGCCAATCTGGTGAGGATTTGAGAATCTCTATGAGCTCGGGTGGTGTTTATAATGATGGATATGCCCATACGCAAGCTGCGGATGTCTCAACCCACACATCTGATCCTTATAGAGATTTAGAATTGCATATATCTGGAAGGGTGGTTGCTACAGCCACAAGAGTCCCTCCCAAAAGCTGTTGTGACCGGATACAGAATGAAGACACCGATCACATTCACATTTTTCTGAGCTCTGATGATAATCCTGTGCATACCAGCAAAGGGTTTACTGAAGCTTCCTCTTCAGGCAGTGCTGTGGGATCAAGGATTCTGTTGGGAACTATAACTGGATTGCGGTCTAGCTCAGAAGAAGGGTCTTGCCTTGTCTACAATAGCAGTGGTACCAAAACCCATGTGATTATGAAGCATAGAACACTTCTGGTGAAACACATGCATTGGTATGGATTGGGAGACAATGTTTCTGTATGTGAGTGTAGATGCACAAGAGCACGATTACCACCAAGCAAATTTTGGTTGTTTGAGCCTCGTTGTGGCATGCATGACATAGGCGGTTGGTATGTTGAAACATTTGGTAAAGATAAGAAAGGACGGTTATCACCATCACAGAGATATTGGGATGGCTTTGATGCAAGGGAACAATCTGAGAAGAGACTCCATCCTGCAATGTACCTTCTTGCTATGGGATATAGAACTTTAGATCTTGAAGATGCAAAAAGAAGGAAACATACATTCAGGGATGTTGTCGACAGAAAGCTCTTTAAAATACTCAATTGGTGCAAAAGACTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

524

Amino Acids

59.6

Weight (kDa)

8.13

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 53 - 117 2.2e-17 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
Acc65I GGTACC 1 cut(s) 1163
AccB1I GGYRCC 1 cut(s) 1163
AccI GTMKAC 2 cut(s) 1149, 1527
AccIII TCCGGA 1 cut(s) 655
AclWI GGATC 2 cut(s) 881, 1090
AcsI RAATTY 2 cut(s) 717, 1289
AcuI CTGAAG 3 cut(s) 651, 1050, 1074
AfaI GTAC 3 cut(s) 433, 1165, 1451
AfiI CCNNNNNNNGG 4 cut(s) 102, 593, 1044, 1313
AgsI TTSAA 5 cut(s) 50, 296, 626, 1346, 1487
Alw21I GWGCWC 5 cut(s) 171, 194, 821, 1016, 1273
Alw26I GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
AlwI GGATC 2 cut(s) 881, 1090
Ama87I CYCGRG 1 cut(s) 820
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 3 cut(s) 664, 727, 860
ApoI RAATTY 2 cut(s) 717, 1289
Asp718I GGTACC 1 cut(s) 1163
AspLEI GCGC 2 cut(s) 116, 343
AspS9I GGNCC 6 cut(s) 90, 138, 247, 355, 584, 679
AsuC2I CCSGG 2 cut(s) 245, 394
AsuHPI GGTGA 4 cut(s) 233, 806, 1216, 1371
AvaI CYCGRG 1 cut(s) 820
AvaII GGWCC 5 cut(s) 90, 138, 247, 355, 584
BanI GGYRCC 1 cut(s) 1163
BanII GRGCYC 4 cut(s) 171, 194, 821, 1016
BarI GAAGNNNNNNTAC 2 cut(s) 1492, 1524
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 5 cut(s) 171, 194, 821, 1016, 1273
BbvI GCAGC 3 cut(s) 651, 714, 847
BccI CCATC 7 cut(s) 439, 530, 691, 833, 1390, 1394, 1446
BceAI ACGGC 2 cut(s) 24, 666
BciVI GTATCC 1 cut(s) 966
BcnI CCSGG 2 cut(s) 245, 394
BcoDI GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
BfaI CTAG 1 cut(s) 1122
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 117
BfuI GTATCC 1 cut(s) 966
BglII AGATCT 1 cut(s) 1480
BisI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
BlsI GCNGC 6 cut(s) 38, 307, 574, 666, 729, 862
Bme1390I CCNGG 2 cut(s) 245, 394
Bme18I GGWCC 5 cut(s) 90, 138, 247, 355, 584
BmeT110I CYCGRG 1 cut(s) 820
BmgT120I GGNCC 6 cut(s) 90, 138, 247, 355, 584, 679
BmiI GGNNCC 2 cut(s) 356, 1165
BmrFI CCNGG 2 cut(s) 245, 394
BmsI GCATC 3 cut(s) 1250, 1399, 1480
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 245, 394
BsaBI GATNNNNATC 1 cut(s) 1023
BsaJI CCNNGG 2 cut(s) 59, 393
BsaWI WCCGGW 4 cut(s) 87, 260, 655, 969
BsaXI ACNNNNNCTCC 2 cut(s) 1224, 1254
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 593, 1044, 1313
Bse1I ACTGG 2 cut(s) 22, 1114
Bse3DI GCAATG 2 cut(s) 407, 1452
Bse8I GATNNNNATC 1 cut(s) 1023
BseAI TCCGGA 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 59, 393
BseGI GGATG 6 cut(s) 450, 702, 871, 1405, 1438, 1525
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 4 cut(s) 102, 593, 1044, 1313
BseMI GCAATG 2 cut(s) 407, 1452
BseMII CTCAG 4 cut(s) 380, 1001, 1140, 1416
BseNI ACTGG 2 cut(s) 22, 1114
BseXI GCAGC 3 cut(s) 651, 714, 847
BshFI GGCC 1 cut(s) 681
BshNI GGYRCC 1 cut(s) 1163
BsiHKAI GWGCWC 5 cut(s) 171, 194, 821, 1016, 1273
BsiHKCI CYCGRG 1 cut(s) 820
BsiSI CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
BslFI GGGAC 2 cut(s) 368, 932
BslI CCNNNNNNNGG 4 cut(s) 102, 593, 1044, 1313
BsmAI GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 368, 932
BsnI GGCC 1 cut(s) 681
BsoBI CYCGRG 1 cut(s) 820
Bsp1286I GDGCHC 5 cut(s) 171, 194, 821, 1016, 1273
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 4 cut(s) 886, 991, 1082, 1480
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 4 cut(s) 379, 1002, 1139, 1417
BspEI TCCGGA 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 356, 1165
BspPI GGATC 2 cut(s) 881, 1090
BspT107I GGYRCC 1 cut(s) 1163
BsrDI GCAATG 2 cut(s) 407, 1452
BsrI ACTGG 2 cut(s) 22, 1114
BssECI CCNNGG 2 cut(s) 59, 393
BssMI GATC 4 cut(s) 886, 991, 1082, 1480
BssT1I CCWWGG 1 cut(s) 59
Bst4CI ACNGT 2 cut(s) 54, 1374
Bst6I CTCTTC 4 cut(s) 147, 627, 1069, 1424
BstC8I GCNNGC 3 cut(s) 118, 858, 1319
BstDEI CTNAG 6 cut(s) 172, 366, 706, 1010, 1126, 1425
BstF5I GGATG 6 cut(s) 450, 702, 871, 1405, 1438, 1525
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 2 cut(s) 116, 343
BstKTI GATC 4 cut(s) 889, 994, 1085, 1483
BstMAI GTCTC 5 cut(s) 30, 179, 874, 1227, 1426
BstMBI GATC 4 cut(s) 886, 991, 1082, 1480
BstMWI GCNNNNNNNGC 2 cut(s) 122, 569
BstNSI RCATGY 3 cut(s) 572, 1216, 1321
BstSCI CCNGG 2 cut(s) 243, 392
BstSFI CTRYAG 1 cut(s) 933
BstV1I GCAGC 3 cut(s) 651, 714, 847
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 1480
BstYI RGATCY 1 cut(s) 1480
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 1 cut(s) 681
BtsCI GGATG 6 cut(s) 450, 702, 871, 1405, 1438, 1525
BtsI GCAGTG 2 cut(s) 1078, 1165
BtsIMutI CAGTG 2 cut(s) 1078, 1165
Cac8I GCNNGC 3 cut(s) 118, 858, 1319
CfoI GCGC 2 cut(s) 116, 343
Cfr13I GGNCC 6 cut(s) 90, 138, 247, 355, 584, 679
CpoI CGGWCCG 2 cut(s) 90, 138
CseI GACGC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 432, 1164, 1450
CspCI CAANNNNNGTGG 2 cut(s) 1141, 1176
CspI CGGWCCG 2 cut(s) 90, 138
CviAII CATG 5 cut(s) 569, 1175, 1213, 1318, 1322
CviQI GTAC 3 cut(s) 432, 1164, 1450
DdeI CTNAG 6 cut(s) 172, 366, 706, 1010, 1126, 1425
DpnI GATC 4 cut(s) 888, 993, 1084, 1482
DpnII GATC 4 cut(s) 886, 991, 1082, 1480
DraI TTTAAA 1 cut(s) 1543
Eam1104I CTCTTC 4 cut(s) 147, 627, 1069, 1424
EarI CTCTTC 4 cut(s) 147, 627, 1069, 1424
EciI GGCGGA 1 cut(s) 515
Ecl136II GAGCTC 4 cut(s) 169, 192, 819, 1014
Eco130I CCWWGG 1 cut(s) 59
Eco24I GRGCYC 4 cut(s) 171, 194, 821, 1016
Eco47I GGWCC 5 cut(s) 90, 138, 247, 355, 584
Eco53kI GAGCTC 4 cut(s) 169, 192, 819, 1014
Eco57I CTGAAG 3 cut(s) 651, 1050, 1074
Eco88I CYCGRG 1 cut(s) 820
EcoICRI GAGCTC 4 cut(s) 169, 192, 819, 1014
EcoO109I RGGNCCY 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 59
EcoT22I ATGCAT 2 cut(s) 1218, 1323
EcoT38I GRGCYC 4 cut(s) 171, 194, 821, 1016
ErhI CCWWGG 1 cut(s) 59
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
FaqI GGGAC 2 cut(s) 368, 932
FatI CATG 5 cut(s) 568, 1174, 1212, 1317, 1321
FauI CCCGC 1 cut(s) 763
FblI GTMKAC 2 cut(s) 1149, 1527
Fnu4HI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
FokI GGATG 6 cut(s) 457, 709, 878, 1412, 1425, 1532
FriOI GRGCYC 4 cut(s) 171, 194, 821, 1016
Fsp4HI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
FspBI CTAG 1 cut(s) 1122
GlaI GCGC 2 cut(s) 115, 342
GluI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 681
HapII CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
HgaI GACGC 1 cut(s) 201
HhaI GCGC 2 cut(s) 116, 343
Hin1II CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
Hin6I GCGC 2 cut(s) 114, 341
HinP1I GCGC 2 cut(s) 114, 341
HincII GTYRAC 2 cut(s) 132, 1528
HindII GTYRAC 2 cut(s) 132, 1528
HindIII AAGCTT 2 cut(s) 374, 1056
HinfI GANTC 9 cut(s) 128, 238, 269, 288, 316, 807, 945, 1090, 1434
HpaII CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
HphI GGTGA 4 cut(s) 233, 806, 1216, 1371
Hpy166II GTNNAC 5 cut(s) 132, 147, 1050, 1150, 1528
Hpy188III TCNNGA 6 cut(s) 587, 614, 656, 722, 918, 1484
Hpy8I GTNNAC 5 cut(s) 132, 147, 1050, 1150, 1528
Hpy99I CGWCG 2 cut(s) 217, 278
HpyAV CCTTC 6 cut(s) 15, 502, 915, 1127, 1463, 1494
HpyCH4III ACNGT 2 cut(s) 54, 1374
HpyCH4IV ACGT 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 569
HpyF3I CTNAG 6 cut(s) 172, 366, 706, 1010, 1126, 1425
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
HspAI GCGC 2 cut(s) 114, 341
Kpn2I TCCGGA 1 cut(s) 655
KpnI GGTACC 1 cut(s) 1167
Kzo9I GATC 4 cut(s) 886, 991, 1082, 1480
Lsp1109I GCAGC 3 cut(s) 651, 714, 847
LweI GCATC 3 cut(s) 1250, 1399, 1480
MaeI CTAG 1 cut(s) 1122
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 263, 965
MalI GATC 4 cut(s) 888, 993, 1084, 1482
MboI GATC 4 cut(s) 886, 991, 1082, 1480
MboII GAAGA 8 cut(s) 164, 602, 644, 995, 1056, 1142, 1441, 1499
MfeI CAATTG 1 cut(s) 1552
MflI RGATCY 1 cut(s) 1480
MhlI GDGCHC 5 cut(s) 171, 194, 821, 1016, 1273
MluCI AATT 7 cut(s) 45, 224, 477, 717, 905, 1289, 1552
MlyI GAGTC 4 cut(s) 137, 232, 954, 1428
MmeI TCCRAC 2 cut(s) 297, 499
Mph1103I ATGCAT 2 cut(s) 1218, 1323
MroI TCCGGA 1 cut(s) 655
MseI TTAA 3 cut(s) 752, 1542, 1573
MslI CAYNNNNRTG 1 cut(s) 1221
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 7 cut(s) 88, 96, 245, 261, 393, 656, 970
MspR9I CCNGG 2 cut(s) 245, 394
MunI CAATTG 1 cut(s) 1552
MwoI GCNNNNNNNGC 2 cut(s) 122, 569
NciI CCSGG 2 cut(s) 245, 394
NdeII GATC 4 cut(s) 886, 991, 1082, 1480
NlaIII CATG 5 cut(s) 572, 1178, 1216, 1321, 1325
NlaIV GGNNCC 2 cut(s) 356, 1165
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 965
NsiI ATGCAT 2 cut(s) 1218, 1323
NspI RCATGY 3 cut(s) 572, 1216, 1321
PaeI GCATGC 1 cut(s) 1321
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 5 cut(s) 269, 288, 316, 807, 1090
PkrI GCNGC 6 cut(s) 38, 307, 574, 666, 729, 862
PleI GAGTC 4 cut(s) 136, 232, 953, 1428
PpsI GAGTC 4 cut(s) 136, 232, 953, 1428
PpuMI RGGWCCY 1 cut(s) 355
PsiI TTATAA 1 cut(s) 834
Psp124BI GAGCTC 4 cut(s) 171, 194, 821, 1016
Psp5II RGGWCCY 1 cut(s) 355
PspN4I GGNNCC 2 cut(s) 356, 1165
PspPI GGNCC 6 cut(s) 90, 138, 247, 355, 584, 679
PspPPI RGGWCCY 1 cut(s) 355
PsuI RGATCY 1 cut(s) 1480
RsaI GTAC 3 cut(s) 433, 1165, 1451
RsaNI GTAC 3 cut(s) 432, 1164, 1450
RseI CAYNNNNRTG 1 cut(s) 1221
Rsr2I CGGWCCG 2 cut(s) 90, 138
RsrII CGGWCCG 2 cut(s) 90, 138
SacI GAGCTC 4 cut(s) 171, 194, 821, 1016
SalI GTCGAC 1 cut(s) 1526
SaqAI TTAA 3 cut(s) 752, 1542, 1573
SatI GCNGC 6 cut(s) 37, 306, 573, 665, 728, 861
Sau3AI GATC 4 cut(s) 886, 991, 1082, 1480
Sau96I GGNCC 6 cut(s) 90, 138, 247, 355, 584, 679
SchI GAGTC 4 cut(s) 137, 232, 954, 1428
ScrFI CCNGG 2 cut(s) 245, 394
SduI GDGCHC 5 cut(s) 171, 194, 821, 1016, 1273
SfaNI GCATC 3 cut(s) 1250, 1399, 1480
SfcI CTRYAG 1 cut(s) 933
SinI GGWCC 5 cut(s) 90, 138, 247, 355, 584
SmiMI CAYNNNNRTG 1 cut(s) 1221
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SphI GCATGC 1 cut(s) 1321
Sse9I AATT 7 cut(s) 45, 224, 477, 717, 905, 1289, 1552
SspMI CTAG 1 cut(s) 1122
SstI GAGCTC 4 cut(s) 171, 194, 821, 1016
StyD4I CCNGG 2 cut(s) 243, 392
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 54, 1374
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 603, 1527
TaqII GACCGA 1 cut(s) 155
TasI AATT 7 cut(s) 45, 224, 477, 717, 905, 1289, 1552
TatI WGTACW 1 cut(s) 431
TauI GCSGC 3 cut(s) 39, 308, 575
TfiI GAWTC 5 cut(s) 269, 288, 316, 807, 1090
Tru1I TTAA 3 cut(s) 752, 1542, 1573
Tru9I TTAA 3 cut(s) 752, 1542, 1573
TscAI CASTG 2 cut(s) 1078, 1165
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 3 cut(s) 664, 727, 860
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 5 cut(s) 209, 416, 663, 996, 1199
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 2 cut(s) 1078, 1165
VpaK11BI GGWCC 5 cut(s) 90, 138, 247, 355, 584
XapI RAATTY 2 cut(s) 717, 1289
XceI RCATGY 3 cut(s) 572, 1216, 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1290
XmiI GTMKAC 2 cut(s) 1149, 1527
XspI CTAG 1 cut(s) 1122
Zsp2I ATGCAT 2 cut(s) 1218, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.