MD15G1333200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017138
Physical Location & Seq
Forward (+)
36971334 .. 36974774
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1333200.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCTGCAAAGGCTGTTTCTGCTTCCAGTTTATTGGTAGCCTGCTCCTCTTCCTCCTCCGACTTTCATCGTCCCCGCCAATTGCGAATCGGAGCTTCTTCCTCTGCTGTTCCTTCTGGTGTCGACCTCAACACCCTCCAATCCGCCATTGCAAAGAAAGATAGCAGTGCGGTTAAACAGGCACTTGATCAGCTAAGTGACATTGGTTGGGCCAAGAAATGGAGTTCTCAGCCATATGTCTCGCGGCGTACGACATCGCTTAGGGAGCTGACAACTCTTGGCATTAAAAATGCAGAAACTCTTGCAGTTCCCAGTGTCAGAAATGATGCGGCCTTTCTATTCACCGTGGTAGGGACAACGGGATTCTTAGGTCTTCTTGCTGGCCAACTTCCTGGGGATTGGGGGTTTTTCGTACCATACTTGCTCGGGAGCATCTCTTTAATAGTTTTGGGTGTGGGAAGCACTGCCCCTGGGCTTCTTCAAGCTGCGATATCTGGCTTTTCATCATTTTTCCCAGACTATCAGGAGAGAATTGCTAGGCATGAAGCTGCTCATTTCTTAGTTGCATATTTGCTGGGCCTTCCTATTCTGGGATACTCTCTAGATATTGGGAAAGAGCATGTCAACCTTATTGATGAAAGGCTCGAGAAGCTGATATACAGCGGGCAGCTCGATGCAAAGGAACTAGACAGGTTGGCGGTGGTAGCAATGGCTGGACTTGCAGCAGAAGGTCTGAAATATGACAAAGTGATCGGTCAATCTGCTGATCTTTTCACTCTACAGAGATTTATCAACAGAAGCAAGCCGCAGCTCAGCAAAGATCAGCAGCAAAATCTTACTAGATGGGCTGTTTTGTTTGCCGGATCGCTCTTAAAAAATGATAAGGTGATTCATGAAGCCTTAATAACAGCAATGTCAAACAAGGCAACTATCCTGGAATGCATTGAAGCAATTGAGAAAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

34.61

Weight (kDa)

8.74

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21960
fragaria_vesca FvH4_1g22260
malus_domestica MD15G1333200.v1.1
prunus_persica Prupe.6G173100_v2.0.a1
pyrus_communis pycom15g29240
rosa_chinensis RchiOBHm_Chr2g0116401
rosa_laevigata RLG00000018266
rosa_multiflora Rmu_sc0004191.1_g000066
rosa_roxburghii Rroxscaffold_2G00127240
rosa_rugosa Rorug02G0206300
rosa_samantha Rh2BG273200 Rh2CG268100
rosa_wichuraiana Rw2G020560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 219
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 244
AciI CCGC 8 cut(s) 76, 144, 170, 244, 329, 663, 698, 806
AclWI GGATC 1 cut(s) 871
AcoI YGGCCR 1 cut(s) 382
AfaI GTAC 2 cut(s) 250, 414
AfiI CCNNNNNNNGG 3 cut(s) 219, 351, 590
AgsI TTSAA 2 cut(s) 482, 947
AjnI CCWGG 3 cut(s) 391, 469, 933
AluBI AGCT 9 cut(s) 95, 193, 268, 485, 548, 652, 670, 811, 962
AluI AGCT 9 cut(s) 95, 193, 268, 485, 548, 652, 670, 811, 962
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 1 cut(s) 871
Ama87I CYCGRG 2 cut(s) 425, 644
AoxI GGCC 4 cut(s) 210, 330, 382, 577
ApeKI GCWGC 8 cut(s) 5, 485, 548, 667, 722, 808, 826, 962
AspS9I GGNCC 2 cut(s) 210, 577
AsuHPI GGTGA 2 cut(s) 334, 898
AvaI CYCGRG 2 cut(s) 425, 644
BalI TGGCCA 1 cut(s) 384
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbsI GAAGAC 1 cut(s) 365
BbvI GCAGC 7 cut(s) 472, 535, 679, 734, 820, 838, 949
BccI CCATC 1 cut(s) 837
BciT130I CCWGG 3 cut(s) 393, 471, 935
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 244
BfaI CTAG 4 cut(s) 537, 602, 686, 840
BfmI CTRYAG 1 cut(s) 779
BfuI GTATCC 1 cut(s) 587
BlpI GCTNAGC 1 cut(s) 812
Bme1390I CCNGG 3 cut(s) 393, 471, 935
BmeT110I CYCGRG 2 cut(s) 425, 644
BmgT120I GGNCC 2 cut(s) 210, 577
BmrFI CCNGG 3 cut(s) 393, 471, 935
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 3 cut(s) 316, 441, 664
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 260
Bpu1102I GCTNAGC 1 cut(s) 812
BsaJI CCNNGG 4 cut(s) 345, 392, 469, 470
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 351, 590
Bse1I ACTGG 2 cut(s) 27, 312
Bse3DI GCAATG 3 cut(s) 147, 714, 918
BseBI CCWGG 3 cut(s) 393, 471, 935
BseDI CCNNGG 4 cut(s) 345, 392, 469, 470
BseLI CCNNNNNNNGG 3 cut(s) 219, 351, 590
BseMI GCAATG 3 cut(s) 147, 714, 918
BseMII CTCAG 2 cut(s) 242, 826
BseNI ACTGG 2 cut(s) 27, 312
BseRI GAGGAG 2 cut(s) 37, 46
BseXI GCAGC 7 cut(s) 472, 535, 679, 734, 820, 838, 949
BseYI CCCAGC 1 cut(s) 574
Bsh1236I CGCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 212, 332, 384, 579
BsiHKCI CYCGRG 2 cut(s) 425, 644
BsiSI CCGG 1 cut(s) 861
BsiWI CGTACG 1 cut(s) 248
BslFI GGGAC 2 cut(s) 57, 367
BslI CCNNNNNNNGG 3 cut(s) 219, 351, 590
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 2 cut(s) 57, 367
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 4 cut(s) 212, 332, 384, 579
BsoBI CYCGRG 2 cut(s) 425, 644
Bsp143I GATC 5 cut(s) 187, 750, 766, 820, 863
Bsp1720I GCTNAGC 1 cut(s) 812
BspACI CCGC 8 cut(s) 76, 144, 170, 244, 329, 663, 698, 806
BspANI GGCC 4 cut(s) 212, 332, 384, 579
BspCNI CTCAG 2 cut(s) 241, 825
BspFNI CGCG 1 cut(s) 244
BspHI TCATGA 1 cut(s) 892
BspPI GGATC 1 cut(s) 871
BsrDI GCAATG 3 cut(s) 147, 714, 918
BsrI ACTGG 2 cut(s) 27, 312
BssECI CCNNGG 4 cut(s) 345, 392, 469, 470
BssMI GATC 5 cut(s) 187, 750, 766, 820, 863
Bst2UI CCWGG 3 cut(s) 393, 471, 935
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 55
BstC8I GCNNGC 4 cut(s) 43, 382, 665, 803
BstDEI CTNAG 6 cut(s) 194, 228, 260, 367, 559, 812
BstDSI CCRYGG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 244
BstKTI GATC 5 cut(s) 190, 753, 769, 823, 866
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 5 cut(s) 187, 750, 766, 820, 863
BstMWI GCNNNNNNNGC 6 cut(s) 11, 20, 265, 649, 704, 719
BstNI CCWGG 3 cut(s) 393, 471, 935
BstNSI RCATGY 1 cut(s) 623
BstSCI CCNGG 3 cut(s) 391, 469, 933
BstSFI CTRYAG 1 cut(s) 779
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 7 cut(s) 472, 535, 679, 734, 820, 838, 949
BstV2I GAAGAC 1 cut(s) 365
BstXI CCANNNNNNTGG 2 cut(s) 34, 392
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 4 cut(s) 212, 332, 384, 579
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 240
BtsI GCAGTG 2 cut(s) 172, 462
BtsIMutI CAGTG 3 cut(s) 172, 319, 462
Cac8I GCNNGC 4 cut(s) 43, 382, 665, 803
CciI TCATGA 1 cut(s) 892
Cfr13I GGNCC 2 cut(s) 210, 577
Csp6I GTAC 2 cut(s) 249, 413
CviAII CATG 3 cut(s) 542, 620, 893
CviQI GTAC 2 cut(s) 249, 413
DdeI CTNAG 6 cut(s) 194, 228, 260, 367, 559, 812
DpnI GATC 5 cut(s) 189, 752, 768, 822, 865
DpnII GATC 5 cut(s) 187, 750, 766, 820, 863
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 133
Eco32I GATATC 1 cut(s) 492
Eco88I CYCGRG 2 cut(s) 425, 644
EcoRII CCWGG 3 cut(s) 391, 469, 933
EcoRV GATATC 1 cut(s) 492
EcoT22I ATGCAT 1 cut(s) 944
FaeI CATG 3 cut(s) 545, 623, 896
FaiI YATR 9 cut(s) 235, 237, 418, 543, 568, 621, 658, 741, 894
FaqI GGGAC 2 cut(s) 57, 367
FatI CATG 3 cut(s) 541, 619, 892
FauI CCCGC 2 cut(s) 83, 656
FauNDI CATATG 1 cut(s) 235
FbaI TGATCA 1 cut(s) 187
FblI GTMKAC 1 cut(s) 123
FspBI CTAG 4 cut(s) 537, 602, 686, 840
GsaI CCCAGC 1 cut(s) 578
HaeIII GGCC 4 cut(s) 212, 332, 384, 579
HapII CCGG 1 cut(s) 861
Hin1II CATG 3 cut(s) 545, 623, 896
HincII GTYRAC 2 cut(s) 124, 625
HindII GTYRAC 2 cut(s) 124, 625
HinfI GANTC 3 cut(s) 87, 363, 889
HpaII CCGG 1 cut(s) 861
HphI GGTGA 2 cut(s) 334, 898
Hpy166II GTNNAC 2 cut(s) 124, 625
Hpy188I TCNGA 4 cut(s) 61, 92, 320, 735
Hpy188III TCNNGA 5 cut(s) 427, 524, 602, 646, 893
Hpy8I GTNNAC 2 cut(s) 124, 625
HpyAV CCTTC 3 cut(s) 123, 590, 722
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 8 cut(s) 8, 152, 293, 305, 566, 677, 722, 942
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 20, 265, 649, 704, 719
HpyF3I CTNAG 6 cut(s) 194, 228, 260, 367, 559, 812
Hsp92II CATG 3 cut(s) 545, 623, 896
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 5 cut(s) 187, 750, 766, 820, 863
LmnI GCTCC 4 cut(s) 50, 92, 265, 429
Lsp1109I GCAGC 7 cut(s) 472, 535, 679, 734, 820, 838, 949
LweI GCATC 3 cut(s) 316, 441, 664
MaeI CTAG 4 cut(s) 537, 602, 686, 840
MaeIII GTNAC 1 cut(s) 197
MalI GATC 5 cut(s) 189, 752, 768, 822, 865
MboI GATC 5 cut(s) 187, 750, 766, 820, 863
MboII GAAGA 4 cut(s) 42, 90, 365, 470
MfeI CAATTG 2 cut(s) 80, 951
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 3 cut(s) 80, 531, 951
MluNI TGGCCA 1 cut(s) 384
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 6 cut(s) 58, 64, 67, 112, 137, 146
Mox20I TGGCCA 1 cut(s) 384
Mph1103I ATGCAT 1 cut(s) 944
MscI TGGCCA 1 cut(s) 384
MseI TTAA 5 cut(s) 174, 285, 440, 872, 902
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 663
MspI CCGG 1 cut(s) 861
MspR9I CCNGG 3 cut(s) 393, 471, 935
MunI CAATTG 2 cut(s) 80, 951
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 3 cut(s) 393, 471, 935
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 6 cut(s) 11, 20, 265, 649, 704, 719
NdeI CATATG 1 cut(s) 235
NdeII GATC 5 cut(s) 187, 750, 766, 820, 863
NlaIII CATG 3 cut(s) 545, 623, 896
NmuCI GTSAC 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 944
NspI RCATGY 1 cut(s) 623
PaeR7I CTCGAG 1 cut(s) 644
PagI TCATGA 1 cut(s) 892
PasI CCCWGGG 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 1 cut(s) 944
PfeI GAWTC 3 cut(s) 87, 363, 889
Pfl23II CGTACG 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 219
PfoI TCCNGGA 1 cut(s) 933
Psp6I CCWGG 3 cut(s) 391, 469, 933
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 3 cut(s) 391, 469, 933
PspLI CGTACG 1 cut(s) 248
PspPI GGNCC 2 cut(s) 210, 577
RsaI GTAC 2 cut(s) 250, 414
RsaNI GTAC 2 cut(s) 249, 413
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 5 cut(s) 174, 285, 440, 872, 902
Sau3AI GATC 5 cut(s) 187, 750, 766, 820, 863
Sau96I GGNCC 2 cut(s) 210, 577
ScrFI CCNGG 3 cut(s) 393, 471, 935
SfaNI GCATC 3 cut(s) 316, 441, 664
SfcI CTRYAG 1 cut(s) 779
Sfr274I CTCGAG 1 cut(s) 644
SlaI CTCGAG 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 3 cut(s) 80, 531, 951
SsiI CCGC 8 cut(s) 76, 144, 170, 244, 329, 663, 698, 806
SspMI CTAG 4 cut(s) 537, 602, 686, 840
StyD4I CCNGG 3 cut(s) 391, 469, 933
TaaI ACNGT 1 cut(s) 346
TaqI TCGA 3 cut(s) 123, 645, 672
TaqII GACCGA 1 cut(s) 743
TasI AATT 3 cut(s) 80, 531, 951
TauI GCSGC 3 cut(s) 247, 332, 808
TfiI GAWTC 3 cut(s) 87, 363, 889
Tru1I TTAA 5 cut(s) 174, 285, 440, 872, 902
Tru9I TTAA 5 cut(s) 174, 285, 440, 872, 902
TscAI CASTG 3 cut(s) 172, 319, 469
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 8 cut(s) 5, 485, 548, 667, 722, 808, 826, 962
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 6 cut(s) 56, 492, 558, 651, 881, 909
TspRI CASTG 3 cut(s) 172, 319, 469
Van91I CCANNNNNTGG 1 cut(s) 219
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 4 cut(s) 537, 602, 686, 840
Zsp2I ATGCAT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.