Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012087

Physical Location & Seq

Genomic Coordinates Forward (+)

40148032 .. 40152322

Gene Structure 4291 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1344500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGGCGCCACTGGTTCAGAGCTCTCAGCCATGGGTCGAAAAATATCGGCCGAGGCAAGTGAAAGATGTGGCGCAGCAGGACGAGGTGGTCCGAGTCCTCACCAACACCCTCGAGACATCAAATTGTCCTCACATGCTCTTCTATGGTCCGCCGGGTACTGGAAAAACCACCACCGCTCTCGCCATCGCTCACCAGCTTTACGGACCTGAACTCTACAAGTCTAGAGTGCTGGAGCTCAATGCTAGTGATGATCGTGGGATCAATGTTGTTCGGACAAAGATCAAAGATTTTGCTGCTGTTGCTGTGGGTACTAATCAACGTCCAGGGGGTTACCCGTGTCCACCATTTAAGATCATAATCCTTGACGAGGCAGATTCAATGACAGAAGATGCTCAGAATGCCCTAAGACGTACAATGGAAACTCACTCCAAAGTCACCCGATTCTTTTTTATATGCAATTATATCAGCAGGATCATAGAGCCACTTGCTTCGAGATGTGCAAAGTTCAGGTTTAAGCCACTTTCAGAAGATATCATGATCACCCGCGTATTGCATATTTGCCAAGAAGAAGGCCTTAACCTGGATCCAGAGGCCCTGTCAACACTAAGCTCTATCTCACAAGGTGATCTTCGTCGGGCTATTACATACTTGCAGTCAGCTGCTCGCTTATTTGGATCTTCTATTTCTTCAAAGGAGCTGATTAGTGTGTCTGGGGTAGTCCCAGAGGAGGTTGTTGAGGCATTTTTCTCTGCGTGCAGGGGTGATAACTTTGACTTGGCAAACAAGGAAGTCAACAATGTAATTGCAGAGGGATATCCGGTCTCTCAAATGCTTTCACAGTTATTTGAGGTGGTGGTTCAGGCAGATGACATATTAGATGAACAGAAGGCTAGAATATGCAAGAAATTGGGCGAAGCAGATAAGTGTCTTGTGGATGGTGCTGACGAGTACTTGCAGCTGCTTGACGTGGCCAGCAGTATGATGCGAGCTGTTTGTAACATGCAGGAAGATTTCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031390 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051338 GO:0051347 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:1900262 GO:1900264 GO:1901360 GO:1901576 GO:2000112 GO:2000278 GO:2000573

KEGG Pathways

Metabolic & Signaling

ko03030 ko03420 ko03430 map03030 map03420 map03430

Pfam Domains

Protein Families

AAA Rep_fac_C

Protein Analysis

340

Amino Acids

37.71

Weight (kDa)

5.15

Isoelectric Point (pI)

38.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	26 - 174	7.7e-11	DNA polymerase III, delta subunit
AAA	PF00004	45 - 173	1.4e-17	ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1	PF25361	181 - 220	2e-07	RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid	PF21960	185 - 222	4e-08	RCF1/5-like, AAA+ ATPase lid domain
Rep_fac_C	PF08542	240 - 325	1.5e-20	Replication factor C C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012087)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21690 AT1G21690 AT1G21690 AT1G21690
fragaria_vesca	FvH4_2g27570 FvH4_2g27570
malus_domestica	MD15G1344500.v1.1
prunus_persica	Prupe.1G492200_v2.0.a1 Prupe.1G492200_v2.0.a1
pyrus_communis	pycom15g30770
rosa_chinensis	RchiOBHm_Chr6g0296411
rosa_laevigata	RLG00000011690
rosa_multiflora	Rmu_sc0008096.1_g000015
rosa_roxburghii	Rroxscaffold_7G00171570 Rroxscaffold_7G00178550
rosa_rugosa	Rorug06G0262100 Rorug06G0262200
rosa_samantha	Rh6BG381800
rosa_wichuraiana	Rw6G032570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	86
AccB1I	GGYRCC	1 cut(s)	4
AccBSI	CCGCTC	1 cut(s)	176
AccII	CGCG	1 cut(s)	546
AciI	CCGC	3 cut(s)	149, 174, 544
AclWI	GGATC	5 cut(s)	266, 479, 578, 591, 682
AcoI	YGGCCR	2 cut(s)	47, 969
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	1 cut(s)	623
AfaI	GTAC	4 cut(s)	157, 310, 412, 950
AfiI	CCNNNNNNNGG	4 cut(s)	158, 367, 580, 727
AgsI	TTSAA	2 cut(s)	378, 690
AjiI	CACGTC	1 cut(s)	967
AjnI	CCWGG	2 cut(s)	322, 579
AluBI	AGCT	8 cut(s)	21, 196, 235, 609, 659, 697, 958, 989
AluI	AGCT	8 cut(s)	21, 196, 235, 609, 659, 697, 958, 989
Alw21I	GWGCWC	2 cut(s)	23, 237
Alw26I	GTCTC	2 cut(s)	107, 826
AlwI	GGATC	5 cut(s)	266, 479, 578, 591, 682
Ama87I	CYCGRG	1 cut(s)	110
AoxI	GGCC	4 cut(s)	47, 571, 591, 969
ApeKI	GCWGC	5 cut(s)	73, 293, 659, 955, 958
AspLEI	GCGC	2 cut(s)	7, 73
AspS9I	GGNCC	4 cut(s)	88, 146, 203, 592
AsuC2I	CCSGG	1 cut(s)	153
AsuHPI	GGTGA	6 cut(s)	91, 182, 427, 532, 635, 773
AvaI	CYCGRG	1 cut(s)	110
AvaII	GGWCC	3 cut(s)	88, 146, 203
BalI	TGGCCA	1 cut(s)	971
BamHI	GGATCC	1 cut(s)	583
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	23, 237
Bbv12I	GWGCWC	2 cut(s)	23, 237
BbvI	GCAGC	5 cut(s)	85, 280, 646, 945, 967
BccI	CCATC	2 cut(s)	191, 929
BciT130I	CCWGG	2 cut(s)	324, 581
BclI	TGATCA	1 cut(s)	537
BcnI	CCSGG	1 cut(s)	153
BcoDI	GTCTC	2 cut(s)	107, 826
BfaI	CTAG	3 cut(s)	222, 243, 891
BfoI	RGCGCY	1 cut(s)	8
BisI	GCNGC	5 cut(s)	74, 294, 660, 956, 959
BlsI	GCNGC	5 cut(s)	75, 295, 661, 957, 960
BmcAI	AGTACT	1 cut(s)	950
Bme1390I	CCNGG	3 cut(s)	153, 324, 581
Bme18I	GGWCC	3 cut(s)	88, 146, 203
BmeT110I	CYCGRG	1 cut(s)	110
BmgBI	CACGTC	1 cut(s)	967
BmgT120I	GGNCC	4 cut(s)	88, 146, 203, 592
BmiI	GGNNCC	2 cut(s)	6, 585
BmrFI	CCNGG	3 cut(s)	153, 324, 581
BmsI	GCATC	2 cut(s)	379, 972
BpmI	CTGGAG	1 cut(s)	251
BpuMI	CCSGG	1 cut(s)	153
BsaBI	GATNNNNATC	1 cut(s)	356
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	826
BsaJI	CCNNGG	3 cut(s)	29, 50, 323
BsaWI	WCCGGW	1 cut(s)	817
Bsc4I	CCNNNNNNNGG	4 cut(s)	158, 367, 580, 727
Bse1I	ACTGG	2 cut(s)	15, 163
Bse8I	GATNNNNATC	1 cut(s)	356
BseBI	CCWGG	2 cut(s)	324, 581
BseDI	CCNNGG	3 cut(s)	29, 50, 323
BseGI	GGATG	1 cut(s)	940
BseJI	GATNNNNATC	1 cut(s)	356
BseLI	CCNNNNNNNGG	4 cut(s)	158, 367, 580, 727
BseMII	CTCAG	2 cut(s)	38, 407
BseNI	ACTGG	2 cut(s)	15, 163
BseRI	GAGGAG	1 cut(s)	740
BseX3I	CGGCCG	1 cut(s)	47
BseXI	GCAGC	5 cut(s)	85, 280, 646, 945, 967
BsgI	GTGCAG	1 cut(s)	775
Bsh1236I	CGCG	1 cut(s)	546
Bsh1285I	CGRYCG	1 cut(s)	50
BshFI	GGCC	4 cut(s)	49, 573, 593, 971
BshNI	GGYRCC	1 cut(s)	4
BsiEI	CGRYCG	1 cut(s)	50
BsiHKAI	GWGCWC	2 cut(s)	23, 237
BsiHKCI	CYCGRG	1 cut(s)	110
BsiSI	CCGG	2 cut(s)	152, 818
BslFI	GGGAC	1 cut(s)	704
BslI	CCNNNNNNNGG	4 cut(s)	158, 367, 580, 727
BsmAI	GTCTC	2 cut(s)	107, 826
BsmFI	GGGAC	1 cut(s)	704
BsmI	GAATGC	1 cut(s)	403
BsnI	GGCC	4 cut(s)	49, 573, 593, 971
Bso31I	GGTCTC	1 cut(s)	826
BsoBI	CYCGRG	1 cut(s)	110
Bsp1286I	GDGCHC	2 cut(s)	23, 237
Bsp143I	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
Bsp19I	CCATGG	1 cut(s)	29
BspACI	CCGC	3 cut(s)	149, 174, 544
BspANI	GGCC	4 cut(s)	49, 573, 593, 971
BspCNI	CTCAG	2 cut(s)	37, 406
BspFNI	CGCG	1 cut(s)	546
BspHI	TCATGA	1 cut(s)	534
BspLI	GGNNCC	2 cut(s)	6, 585
BspPI	GGATC	5 cut(s)	266, 479, 578, 591, 682
BspQI	GCTCTTC	1 cut(s)	143
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	1 cut(s)	826
BsrBI	CCGCTC	1 cut(s)	176
BsrI	ACTGG	2 cut(s)	15, 163
BssECI	CCNNGG	3 cut(s)	29, 50, 323
BssMI	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	29
Bst2UI	CCWGG	2 cut(s)	324, 581
Bst4CI	ACNGT	1 cut(s)	840
Bst6I	CTCTTC	1 cut(s)	143
BstACI	GRCGYC	1 cut(s)	5
BstC8I	GCNNGC	4 cut(s)	664, 754, 973, 987
BstDEI	CTNAG	5 cut(s)	24, 393, 404, 605, 1017
BstDSI	CCRYGG	1 cut(s)	29
BstEII	GGTNACC	1 cut(s)	329
BstENI	CCTNNNNNAGG	1 cut(s)	365
BstF5I	GGATG	1 cut(s)	940
BstFNI	CGCG	1 cut(s)	546
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 73
BstKTI	GATC	9 cut(s)	253, 261, 282, 354, 474, 540, 586, 628, 677
BstMAI	GTCTC	2 cut(s)	107, 826
BstMBI	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
BstMCI	CGRYCG	1 cut(s)	50
BstMWI	GCNNNNNNNGC	2 cut(s)	299, 398
BstNI	CCWGG	2 cut(s)	324, 581
BstNSI	RCATGY	2 cut(s)	136, 1003
BstPI	GGTNACC	1 cut(s)	329
BstSCI	CCNGG	3 cut(s)	151, 322, 579
BstUI	CGCG	1 cut(s)	546
BstV1I	GCAGC	5 cut(s)	85, 280, 646, 945, 967
BstX2I	RGATCY	2 cut(s)	583, 674
BstYI	RGATCY	2 cut(s)	583, 674
BstZI	CGGCCG	1 cut(s)	47
BsuRI	GGCC	4 cut(s)	49, 573, 593, 971
BtgI	CCRYGG	1 cut(s)	29
BtgZI	GCGATG	1 cut(s)	169
BtrI	CACGTC	1 cut(s)	967
BtsCI	GGATG	1 cut(s)	940
BtsIMutI	CAGTG	1 cut(s)	8
Cac8I	GCNNGC	4 cut(s)	664, 754, 973, 987
CciI	TCATGA	1 cut(s)	534
CfoI	GCGC	2 cut(s)	7, 73
Cfr13I	GGNCC	4 cut(s)	88, 146, 203, 592
Csp6I	GTAC	4 cut(s)	156, 309, 411, 949
CviAII	CATG	4 cut(s)	30, 133, 535, 1000
CviQI	GTAC	4 cut(s)	156, 309, 411, 949
DdeI	CTNAG	5 cut(s)	24, 393, 404, 605, 1017
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	9 cut(s)	252, 260, 281, 353, 473, 539, 585, 627, 676
DpnII	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
DraIII	CACNNNGTG	1 cut(s)	623
DrdI	GACNNNNNNGTC	1 cut(s)	86
DseDI	GACNNNNNNGTC	1 cut(s)	86
EaeI	YGGCCR	2 cut(s)	47, 969
EagI	CGGCCG	1 cut(s)	47
Eam1104I	CTCTTC	1 cut(s)	143
EarI	CTCTTC	1 cut(s)	143
EciI	GGCGGA	1 cut(s)	138
Ecl136II	GAGCTC	2 cut(s)	21, 235
EclXI	CGGCCG	1 cut(s)	47
Eco130I	CCWWGG	1 cut(s)	29
Eco147I	AGGCCT	1 cut(s)	573
Eco24I	GRGCYC	2 cut(s)	23, 237
Eco31I	GGTCTC	1 cut(s)	826
Eco32I	GATATC	2 cut(s)	532, 815
Eco47I	GGWCC	3 cut(s)	88, 146, 203
Eco52I	CGGCCG	1 cut(s)	47
Eco53kI	GAGCTC	2 cut(s)	21, 235
Eco88I	CYCGRG	1 cut(s)	110
Eco91I	GGTNACC	1 cut(s)	329
EcoICRI	GAGCTC	2 cut(s)	21, 235
EcoNI	CCTNNNNNAGG	1 cut(s)	365
EcoO109I	RGGNCCY	1 cut(s)	592
EcoO65I	GGTNACC	1 cut(s)	329
EcoRII	CCWGG	2 cut(s)	322, 579
EcoRV	GATATC	2 cut(s)	532, 815
EcoT14I	CCWWGG	1 cut(s)	29
EcoT38I	GRGCYC	2 cut(s)	23, 237
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	29
FaeI	CATG	4 cut(s)	33, 136, 538, 1003
FalI	AAGNNNNNCTT	4 cut(s)	558, 590, 612, 644
FaqI	GGGAC	1 cut(s)	704
FatI	CATG	4 cut(s)	29, 132, 534, 999
FauI	CCCGC	1 cut(s)	551
FbaI	TGATCA	1 cut(s)	537
Fnu4HI	GCNGC	5 cut(s)	74, 294, 660, 956, 959
FokI	GGATG	1 cut(s)	947
FriOI	GRGCYC	2 cut(s)	23, 237
Fsp4HI	GCNGC	5 cut(s)	74, 294, 660, 956, 959
FspBI	CTAG	3 cut(s)	222, 243, 891
GlaI	GCGC	2 cut(s)	6, 72
GluI	GCNGC	5 cut(s)	74, 294, 660, 956, 959
GsuI	CTGGAG	1 cut(s)	251
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	4 cut(s)	49, 573, 593, 971
HapII	CCGG	2 cut(s)	152, 818
HhaI	GCGC	2 cut(s)	7, 73
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	4 cut(s)	33, 136, 538, 1003
Hin6I	GCGC	2 cut(s)	5, 71
HinP1I	GCGC	2 cut(s)	5, 71
HincII	GTYRAC	2 cut(s)	600, 793
HindII	GTYRAC	2 cut(s)	600, 793
HinfI	GANTC	3 cut(s)	93, 374, 441
HpaII	CCGG	2 cut(s)	152, 818
HphI	GGTGA	6 cut(s)	91, 182, 427, 532, 635, 773
Hpy166II	GTNNAC	3 cut(s)	341, 600, 793
Hpy188I	TCNGA	5 cut(s)	18, 92, 273, 396, 526
Hpy188III	TCNNGA	5 cut(s)	112, 222, 492, 535, 587
Hpy8I	GTNNAC	3 cut(s)	341, 600, 793
Hpy99I	CGWCG	1 cut(s)	636
HpyAV	CCTTC	2 cut(s)	563, 880
HpyCH4III	ACNGT	1 cut(s)	840
HpyCH4IV	ACGT	3 cut(s)	319, 409, 966
HpyCH4V	TGCA	9 cut(s)	456, 500, 553, 652, 756, 806, 900, 955, 1003
HpyF10VI	GCNNNNNNNGC	2 cut(s)	299, 398
HpyF3I	CTNAG	5 cut(s)	24, 393, 404, 605, 1017
HpySE526I	ACGT	3 cut(s)	319, 409, 966
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	4 cut(s)	33, 136, 538, 1003
HspAI	GCGC	2 cut(s)	5, 71
KasI	GGCGCC	1 cut(s)	4
Ksp22I	TGATCA	1 cut(s)	537
Kzo9I	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
LguI	GCTCTTC	1 cut(s)	143
LmnI	GCTCC	2 cut(s)	232, 694
Lsp1109I	GCAGC	5 cut(s)	85, 280, 646, 945, 967
LweI	GCATC	2 cut(s)	379, 972
MaeI	CTAG	3 cut(s)	222, 243, 891
MaeII	ACGT	3 cut(s)	319, 409, 966
MaeIII	GTNAC	3 cut(s)	329, 433, 995
MalI	GATC	9 cut(s)	252, 260, 281, 353, 473, 539, 585, 627, 676
MbiI	CCGCTC	1 cut(s)	176
MboI	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
MboII	GAAGA	8 cut(s)	130, 398, 539, 578, 620, 669, 678, 1019
MflI	RGATCY	2 cut(s)	583, 674
MhlI	GDGCHC	2 cut(s)	23, 237
MlsI	TGGCCA	1 cut(s)	971
MluCI	AATT	4 cut(s)	121, 457, 801, 905
MluNI	TGGCCA	1 cut(s)	971
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	102
Mox20I	TGGCCA	1 cut(s)	971
MscI	TGGCCA	1 cut(s)	971
MseI	TTAA	3 cut(s)	348, 513, 576
Msp20I	TGGCCA	1 cut(s)	971
MspA1I	CMGCKG	2 cut(s)	659, 958
MspI	CCGG	2 cut(s)	152, 818
MspR9I	CCNGG	3 cut(s)	153, 324, 581
Mva1269I	GAATGC	1 cut(s)	403
MvaI	CCWGG	2 cut(s)	324, 581
MvnI	CGCG	1 cut(s)	546
MwoI	GCNNNNNNNGC	2 cut(s)	299, 398
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	153
NcoI	CCATGG	1 cut(s)	29
NdeII	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
NlaIII	CATG	4 cut(s)	33, 136, 538, 1003
NlaIV	GGNNCC	2 cut(s)	6, 585
NmeAIII	GCCGAG	1 cut(s)	75
NmuCI	GTSAC	1 cut(s)	433
NspI	RCATGY	2 cut(s)	136, 1003
PaeR7I	CTCGAG	1 cut(s)	110
PagI	TCATGA	1 cut(s)	534
PceI	AGGCCT	1 cut(s)	573
PciSI	GCTCTTC	1 cut(s)	143
PctI	GAATGC	1 cut(s)	403
PfeI	GAWTC	2 cut(s)	374, 441
PkrI	GCNGC	5 cut(s)	75, 295, 661, 957, 960
PleI	GAGTC	1 cut(s)	101
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	1 cut(s)	101
Psp124BI	GAGCTC	2 cut(s)	23, 237
Psp6I	CCWGG	2 cut(s)	322, 579
PspEI	GGTNACC	1 cut(s)	329
PspGI	CCWGG	2 cut(s)	322, 579
PspN4I	GGNNCC	2 cut(s)	6, 585
PspPI	GGNCC	4 cut(s)	88, 146, 203, 592
PsuI	RGATCY	2 cut(s)	583, 674
PvuII	CAGCTG	2 cut(s)	659, 958
RsaI	GTAC	4 cut(s)	157, 310, 412, 950
RsaNI	GTAC	4 cut(s)	156, 309, 411, 949
SacI	GAGCTC	2 cut(s)	23, 237
SapI	GCTCTTC	1 cut(s)	143
SaqAI	TTAA	3 cut(s)	348, 513, 576
SatI	GCNGC	5 cut(s)	74, 294, 660, 956, 959
Sau3AI	GATC	9 cut(s)	250, 258, 279, 351, 471, 537, 583, 625, 674
Sau96I	GGNCC	4 cut(s)	88, 146, 203, 592
ScaI	AGTACT	1 cut(s)	950
SchI	GAGTC	1 cut(s)	102
ScrFI	CCNGG	3 cut(s)	153, 324, 581
SduI	GDGCHC	2 cut(s)	23, 237
SfaNI	GCATC	2 cut(s)	379, 972
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	110
SinI	GGWCC	3 cut(s)	88, 146, 203
SlaI	CTCGAG	1 cut(s)	110
SmlI	CTYRAG	1 cut(s)	110
SmoI	CTYRAG	1 cut(s)	110
Sse9I	AATT	4 cut(s)	121, 457, 801, 905
SseBI	AGGCCT	1 cut(s)	573
SsiI	CCGC	3 cut(s)	149, 174, 544
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	3 cut(s)	222, 243, 891
SstI	GAGCTC	2 cut(s)	23, 237
StuI	AGGCCT	1 cut(s)	573
StyD4I	CCNGG	3 cut(s)	151, 322, 579
StyI	CCWWGG	1 cut(s)	29
TaaI	ACNGT	1 cut(s)	840
TaiI	ACGT	3 cut(s)	322, 412, 969
TaqI	TCGA	3 cut(s)	36, 111, 491
TasI	AATT	4 cut(s)	121, 457, 801, 905
TatI	WGTACW	1 cut(s)	948
TfiI	GAWTC	2 cut(s)	374, 441
Tru1I	TTAA	3 cut(s)	348, 513, 576
Tru9I	TTAA	3 cut(s)	348, 513, 576
TscAI	CASTG	1 cut(s)	15
TseFI	GTSAC	1 cut(s)	433
TseI	GCWGC	5 cut(s)	73, 293, 659, 955, 958
Tsp45I	GTSAC	1 cut(s)	433
TspDTI	ATGAA	1 cut(s)	894
TspGWI	ACGGA	1 cut(s)	216
TspRI	CASTG	1 cut(s)	15
VpaK11BI	GGWCC	3 cut(s)	88, 146, 203
XagI	CCTNNNNNAGG	1 cut(s)	365
XbaI	TCTAGA	1 cut(s)	221
XceI	RCATGY	2 cut(s)	136, 1003
XhoI	CTCGAG	1 cut(s)	110
XspI	CTAG	3 cut(s)	222, 243, 891
ZrmI	AGTACT	1 cut(s)	950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.