MD16G1007300.v1.1

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014231
Physical Location & Seq
Reverse (-)
550143 .. 555164
5022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1007300.v1.1.491

Sequence Viewer

Length: 2130 bp
ATGGATGTTTCCACCAATGGAATCGGGAACAATGTCGTTGAGGTCAGAGTTTGTAGACCCGCCGGAAAGTTAAACGGCGTGCCGGCGAATGGGGTTTTCCCGGGAAGTCCCAGAAAGCCCGAGAAAACAATAAAGCGTTCAAATTCTATCCCCAACGGTATACCGAAACAGAAAGATTACGAGGAGGAAGAGGGAGATGGCTTTTCGGAAGAAGTTGAAATTGCACATGCAAATGGAAATGGTTACCTGAAAGAACGGGACGACGACGTTTCGCCCACCTCGATGACCGAAAAGTCGGACGAGTTTCCAACGGTTTCTCCCGAAAAAGCCGAGTACGTGAAATTAGCCGTTGAGACGCCCGATACAGCAGGGGAAGGGATCCTCCGTCCTCACGCGCACCTGCCGAAGCCAGAGGCGCCGCCCGGGTTGGCCTCATCGCCTCCCGATTCTCCAAACGACGAGGCTTCGAATAACCAGAAATTCGGCGTCGACGTGCCGGCGATAGGGAAGCTCATCCGGGAGCGAAGCAGCAATTTCTCGGCCGCGTTCGTGAGGAGGCTGTCGTCGCTGAAGGACCAGCACCTCAATAGCAACGGAGAAGAAGATCTGAAATCGAAAGACGCGACGGCGACGGAGTTCCATCTCTCCGGCCTCAAAGTGACGGTCAAGCTCAAGAGCGAGTCTGACGACCACCAACAGCCACAGGGACAAGCGACGCTGAAAGGTCGAATCAGCTTCTTCTCCCGGTCGAACTGCAGGGACTGCACGGCCGTGCGGAGGTTTCTGAGAGAGAAGGGGCTGAAATTCGTGGAGATCAACATCGACGTGTATCCGAATCGCGAGAAGGAGCTGGTGGAGAGGACGGGTAGCTCGTCTGTACCTCAGATTTTCTTCAACGAGAAGCAGTTCGGAGGACTGGTGGCGCTCAACTCGCTGAGGAACAGCGGCGGATTTGACCAGCGGTTAAAGGAGATGCTCAGCTCGAAATGTCCCGACGACGCGCCTCCGCCGCCGGTTTACGGATTCGACGATCTGGATGAGGAGGAGTTGACGGACCAGATGATGGGGATAGTTAGGGTGTTGCGGCTGAAGTTGCCGATTCAGGACCGTCTGATGAAGATGAAGATCGTCAAGAACTGCTTCGCCGGGAGCGAGATGGTGGAGGTCCTCATTCAGCACTTGGACTGCGGGCGTCGAAAGGCAGTTGAGATTGGAAGGCAGCTTGCCAGGAAGCATTTCATTCATCATGTATTTGGGGAAAATGATTTCGAGGATGGAAACCATTTTTATCGTTTCCTTGAGCACGAACCCTTTATTCCCAAATGCTTTAATTTCCGGGGATCCATAAGTGACAGTGAACCCAAGCCTGCAGCGAAAGTTGGCCAAAGGCTCACCAAGATCATGTCTGCCATACTCGAGTCCTATGCCTCAGATGACAGGCGCCATCTCGATTACATTGGCATTAGCAACAGTGAGGAGTTCCGAAGGTACATAAACTTGGTTCAAGAACTTCACCGAGTAAACCTTTTTGAGCTCTCAAAAGATGAGAGGCTAGCTTTCTTTTTGAACCTCTACAACGCAATGGTCATCCATGCAGTTATCAGGGTAGGACGTCCACAAGGTGTAATCGAAAGGAGATCGTTCTTTTCCGACTTCCAGTATTTAGTTGGAGGCCATCCTTATTCACTCAGTAGTATCGAGAATGGGATCCTCAGAAACAACCGTAGACCTCCTTATTCCTTGGGCAAGCCCTTTGGGGCAGGAGACAACCGTACAGAGCTTGCCTGTGCTAGAGTGAATCCATTAATTCACTTTGGACTTTGTAACGGGACAAGGTCAAGCCCAACGGTAAGATTCTTCTCACCCCAAGGAGTTGAAGCTGAATTGAGATGTGCAGCTAGAGACTTCTTCAAGAGCGGCGGCATGGAAGTGAACTTGGAGAAGAGGACCGTGTACCTCACCCAAATTATTAAATGGTTCGATGGCGACTTTGGACAAGAAAAGGAAATTCTAAAGTGGATCCTGAATTACTTGGATGCAACCAGAGCAGGCCTTTTGACACATCTTTTGGGTGACGGAGGGCATATAAGTATTGTGTACCGGAACTACGATTGGTCCATGAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEP DUF547 Glutaredoxin

Protein Analysis

710

Amino Acids

79.76

Weight (kDa)

6.7

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 245 - 301 3.7e-15 Glutaredoxin
DEP PF00610 365 - 432 5.3e-21 Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)
DUF547 PF04784 511 - 636 2.2e-37 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11920
fragaria_vesca FvH4_4g36430
malus_domestica MD13G1009300.v1.1 MD16G1007300.v1.1
prunus_persica Prupe.1G343400_v2.0.a1
pyrus_communis pycom13g00790 pycom16g00690
rosa_chinensis RchiOBHm_Chr4g0446191
rosa_laevigata RLG00000005684
rosa_multiflora Rmu_sc0009254.1_g000004
rosa_roxburghii Rroxscaffold_5G00386580
rosa_rugosa Rorug04G0368300
rosa_samantha Rh4AG430300 Rh4BG439600 Rh4CG457000 Rh4DG438200
rosa_wichuraiana Rw4G036750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 408
AasI GACNNNNNNGTC 1 cut(s) 292
AatII GACGTC 1 cut(s) 1615
Acc36I ACCTGC 1 cut(s) 408
AccB1I GGYRCC 2 cut(s) 415, 1440
AccB7I CCANNNNNTGG 1 cut(s) 1063
AccBSI CCGCTC 1 cut(s) 1917
AccI GTMKAC 4 cut(s) 55, 160, 489, 1726
AccII CGCG 5 cut(s) 395, 545, 623, 840, 1001
AclWI GGATC 8 cut(s) 373, 386, 1335, 1348, 1702, 1715, 2014, 2027
AcoI YGGCCR 3 cut(s) 540, 768, 1381
AcsI RAATTY 4 cut(s) 142, 479, 803, 2007
AcuI CTGAAG 2 cut(s) 590, 1109
AcyI GRCGYC 6 cut(s) 356, 416, 486, 1192, 1441, 1612
AdeI CACNNNGTG 1 cut(s) 1622
AfaI GTAC 6 cut(s) 335, 879, 1490, 1774, 1955, 2099
AfiI CCNNNNNNNGG 5 cut(s) 89, 503, 777, 1019, 1063
AflIII ACRYGT 1 cut(s) 825
AgsI TTSAA 7 cut(s) 141, 218, 895, 1505, 1567, 1877, 1912
AjiI CACGTC 2 cut(s) 493, 826
AjnI CCWGG 1 cut(s) 1226
AleI CACNNNNGTG 1 cut(s) 770
Alw21I GWGCWC 2 cut(s) 1305, 1536
Alw26I GTCTC 3 cut(s) 347, 1758, 1896
AlwI GGATC 8 cut(s) 373, 386, 1335, 1348, 1702, 1715, 2014, 2027
AlwNI CAGNNNCTG 1 cut(s) 762
Ama87I CYCGRG 4 cut(s) 100, 119, 422, 1415
AoxI GGCC 7 cut(s) 429, 540, 649, 768, 1381, 1672, 2050
ApeKI GCWGC 4 cut(s) 528, 1219, 1370, 1895
ApoI RAATTY 4 cut(s) 142, 479, 803, 2007
ArsI GACNNNNNNTTYG 4 cut(s) 648, 680, 2050, 2082
AseI ATTAAT 1 cut(s) 1805
Asp700I GAANNNNTTC 3 cut(s) 905, 1139, 1235
AspLEI GCGC 5 cut(s) 397, 418, 925, 1003, 1443
AspS9I GGNCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
AsuC2I CCSGG 8 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1337
AsuHPI GGTGA 5 cut(s) 1384, 1505, 1854, 1951, 2084
AsuII TTCGAA 1 cut(s) 467
AsuNHI GCTAGC 1 cut(s) 1552
AvaI CYCGRG 4 cut(s) 100, 119, 422, 1415
AvaII GGWCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
BalI TGGCCA 1 cut(s) 1383
BamHI GGATCC 4 cut(s) 378, 1340, 1707, 2019
BanI GGYRCC 2 cut(s) 415, 1440
BanII GRGCYC 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 1305, 1536
BbvCI CCTCAGC 1 cut(s) 935
BbvI GCAGC 4 cut(s) 540, 1231, 1382, 1907
BccI CCATC 8 cut(s) 191, 648, 1057, 1150, 1268, 1452, 1683, 1976
BceAI ACGGC 5 cut(s) 91, 332, 642, 755, 783
BcgI CGANNNNNNTGC 2 cut(s) 1406, 1440
BciT130I CCWGG 1 cut(s) 1228
BciVI GTATCC 1 cut(s) 840
BcnI CCSGG 8 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1337
BcoDI GTCTC 3 cut(s) 347, 1758, 1896
BfaI CTAG 3 cut(s) 1553, 1791, 1899
BfmI CTRYAG 2 cut(s) 754, 1368
BfoI RGCGCY 3 cut(s) 419, 926, 1444
BfuAI ACCTGC 1 cut(s) 408
BfuI GTATCC 1 cut(s) 840
BglII AGATCT 1 cut(s) 604
BlpI GCTNAGC 1 cut(s) 977
Bme1390I CCNGG 9 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1228, 1337
Bme18I GGWCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
BmeT110I CYCGRG 4 cut(s) 100, 119, 422, 1415
BmgBI CACGTC 2 cut(s) 493, 826
BmgT120I GGNCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
BmiI GGNNCC 6 cut(s) 380, 417, 1342, 1442, 1709, 2021
BmrFI CCNGG 9 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1228, 1337
BmsI GCATC 2 cut(s) 963, 2026
BmtI GCTAGC 1 cut(s) 1556
Bpu10I CCTNAGC 1 cut(s) 935
Bpu1102I GCTNAGC 1 cut(s) 977
Bpu14I TTCGAA 1 cut(s) 467
BpuEI CTTGAG 2 cut(s) 656, 1319
BpuMI CCSGG 8 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1337
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 2 cut(s) 818, 1124
BsaHI GRCGYC 6 cut(s) 356, 416, 486, 1192, 1441, 1612
BsaJI CCNNGG 5 cut(s) 100, 422, 1336, 1740, 1867
BsaWI WCCGGW 1 cut(s) 2100
BsaXI ACNNNNNCTCC 4 cut(s) 301, 331, 903, 933
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 503, 777, 1019, 1063
Bse118I RCCGGY 3 cut(s) 82, 496, 1012
Bse1I ACTGG 2 cut(s) 921, 1657
Bse3DI GCAATG 1 cut(s) 1587
Bse8I GATNNNNATC 2 cut(s) 818, 1124
BseBI CCWGG 1 cut(s) 1228
BseDI CCNNGG 5 cut(s) 100, 422, 1336, 1740, 1867
BseGI GGATG 7 cut(s) 10, 513, 1042, 1279, 1587, 1675, 2041
BseJI GATNNNNATC 2 cut(s) 818, 1124
BseLI CCNNNNNNNGG 5 cut(s) 89, 503, 777, 1019, 1063
BseMI GCAATG 1 cut(s) 1587
BseMII CTCAG 7 cut(s) 776, 896, 926, 991, 1443, 1702, 1726
BseNI ACTGG 2 cut(s) 921, 1657
BseRI GAGGAG 5 cut(s) 197, 568, 1055, 1058, 1490
BseX3I CGGCCG 2 cut(s) 540, 768
BseXI GCAGC 4 cut(s) 540, 1231, 1382, 1907
BsgI GTGCAG 2 cut(s) 748, 1914
Bsh1236I CGCG 5 cut(s) 395, 545, 623, 840, 1001
Bsh1285I CGRYCG 3 cut(s) 543, 749, 771
BshFI GGCC 7 cut(s) 431, 542, 651, 770, 1383, 1674, 2052
BshNI GGYRCC 2 cut(s) 415, 1440
BsiEI CGRYCG 3 cut(s) 543, 749, 771
BsiHKAI GWGCWC 2 cut(s) 1305, 1536
BsiHKCI CYCGRG 4 cut(s) 100, 119, 422, 1415
BslFI GGGAC 6 cut(s) 93, 272, 720, 773, 975, 1843
BslI CCNNNNNNNGG 5 cut(s) 89, 503, 777, 1019, 1063
BsmAI GTCTC 3 cut(s) 347, 1758, 1896
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 6 cut(s) 93, 272, 720, 773, 975, 1843
BsnI GGCC 7 cut(s) 431, 542, 651, 770, 1383, 1674, 2052
BsoBI CYCGRG 4 cut(s) 100, 119, 422, 1415
Bsp119I TTCGAA 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 1305, 1536
Bsp1720I GCTNAGC 1 cut(s) 977
Bsp68I TCGCGA 1 cut(s) 840
BspANI GGCC 7 cut(s) 431, 542, 651, 770, 1383, 1674, 2052
BspCNI CTCAG 7 cut(s) 777, 895, 927, 990, 1442, 1701, 1725
BspFNI CGCG 5 cut(s) 395, 545, 623, 840, 1001
BspLI GGNNCC 6 cut(s) 380, 417, 1342, 1442, 1709, 2021
BspMAI CTGCAG 2 cut(s) 758, 1372
BspMI ACCTGC 1 cut(s) 408
BspOI GCTAGC 1 cut(s) 1556
BspPI GGATC 8 cut(s) 373, 386, 1335, 1348, 1702, 1715, 2014, 2027
BspT104I TTCGAA 1 cut(s) 467
BspT107I GGYRCC 2 cut(s) 415, 1440
BsrBI CCGCTC 1 cut(s) 1917
BsrDI GCAATG 1 cut(s) 1587
BsrFI RCCGGY 3 cut(s) 82, 496, 1012
BsrI ACTGG 2 cut(s) 921, 1657
BssAI RCCGGY 3 cut(s) 82, 496, 1012
BssECI CCNNGG 5 cut(s) 100, 422, 1336, 1740, 1867
BssNAI GTATAC 1 cut(s) 161
BssNI GRCGYC 6 cut(s) 356, 416, 486, 1192, 1441, 1612
BssT1I CCWWGG 2 cut(s) 1740, 1867
Bst1107I GTATAC 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 1228
Bst6I CTCTTC 2 cut(s) 183, 1937
BstACI GRCGYC 6 cut(s) 356, 416, 486, 1192, 1441, 1612
BstAPI GCANNNNNTGC 1 cut(s) 762
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 467
BstDEI CTNAG 7 cut(s) 785, 882, 935, 977, 1429, 1688, 1712
BstEII GGTNACC 1 cut(s) 242
BstF5I GGATG 7 cut(s) 10, 513, 1042, 1279, 1587, 1675, 2041
BstFNI CGCG 5 cut(s) 395, 545, 623, 840, 1001
BstH2I RGCGCY 3 cut(s) 419, 926, 1444
BstHHI GCGC 5 cut(s) 397, 418, 925, 1003, 1443
BstMAI GTCTC 3 cut(s) 347, 1758, 1896
BstMCI CGRYCG 3 cut(s) 543, 749, 771
BstMWI GCNNNNNNNGC 7 cut(s) 415, 565, 762, 931, 1009, 1093, 2045
BstNI CCWGG 1 cut(s) 1228
BstNSI RCATGY 1 cut(s) 230
BstPI GGTNACC 1 cut(s) 242
BstSCI CCNGG 9 cut(s) 99, 100, 421, 422, 516, 743, 1145, 1226, 1335
BstSFI CTRYAG 2 cut(s) 754, 1368
BstUI CGCG 5 cut(s) 395, 545, 623, 840, 1001
BstV1I GCAGC 4 cut(s) 540, 1231, 1382, 1907
BstX2I RGATCY 5 cut(s) 378, 604, 1340, 1707, 2019
BstYI RGATCY 5 cut(s) 378, 604, 1340, 1707, 2019
BstZ17I GTATAC 1 cut(s) 161
BstZI CGGCCG 2 cut(s) 540, 768
BsuI GTATCC 1 cut(s) 840
BsuRI GGCC 7 cut(s) 431, 542, 651, 770, 1383, 1674, 2052
BtgZI GCGATG 1 cut(s) 420
BtrI CACGTC 2 cut(s) 493, 826
BtsCI GGATG 7 cut(s) 10, 513, 1042, 1279, 1587, 1675, 2041
BtsIMutI CAGTG 2 cut(s) 1360, 1477
BtuMI TCGCGA 1 cut(s) 840
BveI ACCTGC 1 cut(s) 408
CaiI CAGNNNCTG 1 cut(s) 762
CfoI GCGC 5 cut(s) 397, 418, 925, 1003, 1443
Cfr10I RCCGGY 3 cut(s) 82, 496, 1012
Cfr13I GGNCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
Cfr9I CCCGGG 2 cut(s) 100, 422
CseI GACGC 6 cut(s) 364, 475, 629, 724, 1007, 1181
Csp6I GTAC 6 cut(s) 334, 878, 1489, 1773, 1954, 2098
CviAII CATG 6 cut(s) 227, 1247, 1402, 1592, 1924, 2119
CviQI GTAC 6 cut(s) 334, 878, 1489, 1773, 1954, 2098
DdeI CTNAG 7 cut(s) 785, 882, 935, 977, 1429, 1688, 1712
DinI GGCGCC 2 cut(s) 417, 1442
DraIII CACNNNGTG 1 cut(s) 1622
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 3 cut(s) 540, 768, 1381
EagI CGGCCG 2 cut(s) 540, 768
Eam1104I CTCTTC 2 cut(s) 183, 1937
EarI CTCTTC 2 cut(s) 183, 1937
EciI GGCGGA 2 cut(s) 963, 996
Ecl136II GAGCTC 1 cut(s) 1534
EclXI CGGCCG 2 cut(s) 540, 768
Eco130I CCWWGG 2 cut(s) 1740, 1867
Eco147I AGGCCT 1 cut(s) 2052
Eco24I GRGCYC 1 cut(s) 1536
Eco47I GGWCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
Eco52I CGGCCG 2 cut(s) 540, 768
Eco53kI GAGCTC 1 cut(s) 1534
Eco57I CTGAAG 2 cut(s) 590, 1109
Eco88I CYCGRG 4 cut(s) 100, 119, 422, 1415
Eco91I GGTNACC 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 1534
EcoO109I RGGNCCY 1 cut(s) 1165
EcoO65I GGTNACC 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 1226
EcoT14I CCWWGG 2 cut(s) 1740, 1867
EcoT38I GRGCYC 1 cut(s) 1536
EgeI GGCGCC 2 cut(s) 417, 1442
EheI GGCGCC 2 cut(s) 417, 1442
ErhI CCWWGG 2 cut(s) 1740, 1867
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 6 cut(s) 230, 1250, 1405, 1595, 1927, 2122
FalI AAGNNNNNCTT 2 cut(s) 1124, 1156
FaqI GGGAC 6 cut(s) 93, 272, 720, 773, 975, 1843
FatI CATG 6 cut(s) 226, 1246, 1401, 1591, 1923, 2118
FauI CCCGC 2 cut(s) 67, 1181
FblI GTMKAC 4 cut(s) 55, 160, 489, 1726
FokI GGATG 7 cut(s) 17, 500, 1049, 1286, 1574, 1662, 2048
FriOI GRGCYC 1 cut(s) 1536
FspBI CTAG 3 cut(s) 1553, 1791, 1899
GlaI GCGC 5 cut(s) 396, 417, 924, 1002, 1442
HaeII RGCGCY 3 cut(s) 419, 926, 1444
HaeIII GGCC 7 cut(s) 431, 542, 651, 770, 1383, 1674, 2052
HgaI GACGC 6 cut(s) 364, 475, 629, 724, 1007, 1181
HhaI GCGC 5 cut(s) 397, 418, 925, 1003, 1443
Hin1I GRCGYC 6 cut(s) 356, 416, 486, 1192, 1441, 1612
Hin1II CATG 6 cut(s) 230, 1250, 1405, 1595, 1927, 2122
Hin6I GCGC 5 cut(s) 395, 416, 923, 1001, 1441
HinP1I GCGC 5 cut(s) 395, 416, 923, 1001, 1441
HincII GTYRAC 2 cut(s) 490, 1050
HindII GTYRAC 2 cut(s) 490, 1050
HphI GGTGA 5 cut(s) 1384, 1505, 1854, 1951, 2084
HpyAV CCTTC 6 cut(s) 368, 565, 787, 838, 1209, 1479
HpyCH4IV ACGT 5 cut(s) 267, 336, 492, 825, 1612
HpyCH4V TGCA 8 cut(s) 224, 230, 756, 765, 1370, 1595, 1895, 2039
HpyF10VI GCNNNNNNNGC 7 cut(s) 415, 565, 762, 931, 1009, 1093, 2045
HpyF3I CTNAG 7 cut(s) 785, 882, 935, 977, 1429, 1688, 1712
HpySE526I ACGT 5 cut(s) 267, 336, 492, 825, 1612
Hsp92I GRCGYC 6 cut(s) 356, 416, 486, 1192, 1441, 1612
Hsp92II CATG 6 cut(s) 230, 1250, 1405, 1595, 1927, 2122
HspAI GCGC 5 cut(s) 395, 416, 923, 1001, 1441
KasI GGCGCC 2 cut(s) 415, 1440
KroI GCCGGC 2 cut(s) 82, 496
KroNI GCCGGC 2 cut(s) 84, 498
LmnI GCTCC 3 cut(s) 520, 847, 1149
Lsp1109I GCAGC 4 cut(s) 540, 1231, 1382, 1907
LweI GCATC 2 cut(s) 963, 2026
MaeI CTAG 3 cut(s) 1553, 1791, 1899
MaeII ACGT 5 cut(s) 267, 336, 492, 825, 1612
MaeIII GTNAC 5 cut(s) 242, 658, 1349, 1823, 2072
MbiI CCGCTC 1 cut(s) 1917
MflI RGATCY 5 cut(s) 378, 604, 1340, 1707, 2019
MhlI GDGCHC 2 cut(s) 1305, 1536
MlsI TGGCCA 1 cut(s) 1383
MluNI TGGCCA 1 cut(s) 1383
Mly113I GGCGCC 2 cut(s) 416, 1441
MlyI GAGTC 2 cut(s) 689, 1427
MmeI TCCRAC 4 cut(s) 276, 332, 1648, 1674
Mox20I TGGCCA 1 cut(s) 1383
MroNI GCCGGC 2 cut(s) 82, 496
MroXI GAANNNNTTC 3 cut(s) 905, 1139, 1235
MscI TGGCCA 1 cut(s) 1383
MseI TTAA 5 cut(s) 71, 965, 1329, 1805, 1971
MslI CAYNNNNRTG 5 cut(s) 231, 281, 770, 824, 1928
Msp20I TGGCCA 1 cut(s) 1383
MspA1I CMGCKG 2 cut(s) 945, 961
MspR9I CCNGG 9 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1228, 1337
MvaI CCWGG 1 cut(s) 1228
MvnI CGCG 5 cut(s) 395, 545, 623, 840, 1001
MwoI GCNNNNNNNGC 7 cut(s) 415, 565, 762, 931, 1009, 1093, 2045
NaeI GCCGGC 2 cut(s) 84, 498
NarI GGCGCC 2 cut(s) 416, 1441
NciI CCSGG 8 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1337
NgoMIV GCCGGC 2 cut(s) 82, 496
NheI GCTAGC 1 cut(s) 1552
NlaIII CATG 6 cut(s) 230, 1250, 1405, 1595, 1927, 2122
NlaIV GGNNCC 6 cut(s) 380, 417, 1342, 1442, 1709, 2021
NmeAIII GCCGAG 2 cut(s) 355, 518
NmuCI GTSAC 3 cut(s) 658, 1349, 2072
NruI TCGCGA 1 cut(s) 840
NspI RCATGY 1 cut(s) 230
NspV TTCGAA 1 cut(s) 467
OliI CACNNNNGTG 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 1415
PaqCI CACCTGC 1 cut(s) 408
PceI AGGCCT 1 cut(s) 2052
PcsI WCGNNNNNNNCGW 6 cut(s) 278, 489, 620, 869, 1026, 1149
PdiI GCCGGC 2 cut(s) 84, 498
PdmI GAANNNNTTC 3 cut(s) 905, 1139, 1235
PfeI GAWTC 8 cut(s) 21, 446, 729, 835, 1023, 1099, 1798, 1854
PflFI GACNNNGTC 1 cut(s) 1834
PflMI CCANNNNNTGG 1 cut(s) 1063
PfoI TCCNGGA 1 cut(s) 516
PleI GAGTC 2 cut(s) 688, 1426
PluTI GGCGCC 2 cut(s) 419, 1444
PpsI GAGTC 2 cut(s) 688, 1426
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 1165
PshBI ATTAAT 1 cut(s) 1805
Psp124BI GAGCTC 1 cut(s) 1536
Psp5II RGGWCCY 1 cut(s) 1165
Psp6I CCWGG 1 cut(s) 1226
PspEI GGTNACC 1 cut(s) 242
PspGI CCWGG 1 cut(s) 1226
PspN4I GGNNCC 6 cut(s) 380, 417, 1342, 1442, 1709, 2021
PspPI GGNCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
PspPPI RGGWCCY 1 cut(s) 1165
PspXI VCTCGAGB 1 cut(s) 1415
PstI CTGCAG 2 cut(s) 758, 1372
PstNI CAGNNNCTG 1 cut(s) 762
PsuI RGATCY 5 cut(s) 378, 604, 1340, 1707, 2019
PsyI GACNNNGTC 1 cut(s) 1834
RruI TCGCGA 1 cut(s) 840
RsaI GTAC 6 cut(s) 335, 879, 1490, 1774, 1955, 2099
RsaNI GTAC 6 cut(s) 334, 878, 1489, 1773, 1954, 2098
RseI CAYNNNNRTG 5 cut(s) 231, 281, 770, 824, 1928
SacI GAGCTC 1 cut(s) 1536
SalI GTCGAC 1 cut(s) 488
SaqAI TTAA 5 cut(s) 71, 965, 1329, 1805, 1971
Sau96I GGNCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
SchI GAGTC 2 cut(s) 689, 1427
ScrFI CCNGG 9 cut(s) 101, 102, 423, 424, 518, 745, 1147, 1228, 1337
SduI GDGCHC 2 cut(s) 1305, 1536
SfaNI GCATC 2 cut(s) 963, 2026
SfcI CTRYAG 2 cut(s) 754, 1368
SfoI GGCGCC 2 cut(s) 417, 1442
Sfr274I CTCGAG 1 cut(s) 1415
SfuI TTCGAA 1 cut(s) 467
SgrDI CGTCGACG 1 cut(s) 488
SinI GGWCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
SlaI CTCGAG 1 cut(s) 1415
SmaI CCCGGG 2 cut(s) 102, 424
SmiMI CAYNNNNRTG 5 cut(s) 231, 281, 770, 824, 1928
SmlI CTYRAG 3 cut(s) 671, 1298, 1415
SmoI CTYRAG 3 cut(s) 671, 1298, 1415
SseBI AGGCCT 1 cut(s) 2052
SspDI GGCGCC 2 cut(s) 415, 1440
SspMI CTAG 3 cut(s) 1553, 1791, 1899
SstI GAGCTC 1 cut(s) 1536
StuI AGGCCT 1 cut(s) 2052
StyD4I CCNGG 9 cut(s) 99, 100, 421, 422, 516, 743, 1145, 1226, 1335
StyI CCWWGG 2 cut(s) 1740, 1867
TaiI ACGT 5 cut(s) 270, 339, 495, 828, 1615
TaqII GACCGA 1 cut(s) 302
TauI GCSGC 7 cut(s) 421, 545, 948, 1012, 1087, 1920, 1923
TfiI GAWTC 8 cut(s) 21, 446, 729, 835, 1023, 1099, 1798, 1854
Tru1I TTAA 5 cut(s) 71, 965, 1329, 1805, 1971
Tru9I TTAA 5 cut(s) 71, 965, 1329, 1805, 1971
TscAI CASTG 2 cut(s) 1360, 1477
TseFI GTSAC 3 cut(s) 658, 1349, 2072
TseI GCWGC 4 cut(s) 528, 1219, 1370, 1895
Tsp45I GTSAC 3 cut(s) 658, 1349, 2072
TspDTI ATGAA 4 cut(s) 1130, 1136, 1228, 1232
TspGWI ACGGA 6 cut(s) 374, 609, 647, 1035, 1067, 2091
TspMI CCCGGG 2 cut(s) 100, 422
TspRI CASTG 2 cut(s) 1360, 1477
Tth111I GACNNNGTC 1 cut(s) 1834
Van91I CCANNNNNTGG 1 cut(s) 1063
VpaK11BI GGWCC 6 cut(s) 574, 1054, 1105, 1165, 1947, 2115
VspI ATTAAT 1 cut(s) 1805
XapI RAATTY 4 cut(s) 142, 479, 803, 2007
XceI RCATGY 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 1664
XhoI CTCGAG 1 cut(s) 1415
XmaI CCCGGG 2 cut(s) 100, 422
XmiI GTMKAC 4 cut(s) 55, 160, 489, 1726
XmnI GAANNNNTTC 3 cut(s) 905, 1139, 1235
XspI CTAG 3 cut(s) 1553, 1791, 1899
ZraI GACGTC 1 cut(s) 1613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.