Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005262

Physical Location & Seq

Genomic Coordinates Forward (+)

898538 .. 901975

Gene Structure 3438 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1011700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGAAGATGAACCTCCCGCCCATTCTCCTACTCGCGGCTCTAATCGCGTGCACTGCCACTCACGTCAGATCCGACGCCTCCGACCACCGCTACAACGAAGGCGACGCCGTCCCCCTCTACGCCAACAAAGTCGGGCCCTTCCACAACCCCAGCGAAACGTATCGGTATTTTGATCTGCCCTTCTGCTCAGCAGGTGATGTGAAGGAGAAAAGGGAGGCGCTTGGAGAGGTGTTGAATGGAGATCGGCTTGTTAGTGCTCCTTATAAGCTTGAGTTTAGAAAAGATAAGGACTCTGAGGTTGCTTGTAGAAGGAAGCTGACCAAGGAAGAAGTTGCGCAGTTTCGAACTGCTGTGAAAAAAGACTACTACTTCCAAATGTATTATGATGACTTGCCCATCTGGGGCTTTTTGGGAAAGGTTGACAAGGAAGGAAAAACTGACCCAAGGGAATACAAATATTTTCTTTACAAGCATATCCAATTTGATGCTCGGTACAATAAAGACCGTGTGATTGAAATCACCGCCAAGATGGACCCTCATTCTGTTGTAGACTTGACGGAGGACAAGGATGTTGATGCGGATTTCATGTACAGTGTGAGATGGTCGGATACTAACATTCCCTTTGAGAATAGAATGGATAAATACTCACAGTCCTCTTCACTGCCACATCACTTGGAAATCCATTGGTTCTCAATCATAAATTCATGCGTCACAGTTCTCCTTTTGACGGGTTTTCTTGCAACGATTCTCATGCGAGTCCTGAAGAATGATTTCATGAAGTATGCACAAGATGAGGAAGCTGCTGATGATCAAGAAGAGACTGGATGGAAGTACATTCATGGTGATGTTTTCCGGTTCCCCAAGAACAAATCTTTATTTGCTGCAGCCCATGGTTCTGGAACCCAGCTGTTCACTCTCACAATATTCATTTTCATGCTGGCATTGGTTGGTGTATTTTATCCATACAACCGAGGAGCTTTATTCACTGCGCTTGTGGTCATATATGCACTTACTTCTGGCATTGCAGGATATACTGCAACTTCTTTCTATTGTCAGCTTGAAGGAAAAAACTGGGTGAGAAACTTGTTACTTACCGGGTGCCTTTTCTGCGGGCCCCTGTTCCTGACTTTCTGCTTCCTTAACACTGTTGCAATTGTTTATAGTGCAACTGCAGCTCTTCCTTTTGGTACTATTGTGGTAATTGTCCTTATTTGGACATTGGTAACATCACCATTGCTTGTCTTGGGTGGTATCGCCGGGAAAAACAGCAAGGCTGAATTTCAAGCTCCTGTCCGCACCACAAAGTATCCTCGAGAAATTCCAACTCTTCCATGGTACAGGACGACCATTCCTCAGATGGCAATGGCAGGGTTTCTCCCTTTCAGTGCAATATACATTGAACTTTACTACATATTTGCCAGCGTCTGGGGTCACAGGATCTACACAATCTACAGCATCCTGTTTATTGTATTTATTATTCTTTTGATTGTCACTGCCTTTATAACTGTGGCTTTGACATATTTCCAACTTGCTGCTGAAGATCATGGGTGGTGGTGGAGGTCTTTCCTATGTGGTGGATCCACTGGCCTCTTCATCTATGGTTACTGCTTGTATTATTACTATGCCCGATCAGATATGTCTGGTTTTATGCAAACATCGTTCTTCTTCGGTTACATGGCCTGCATTTGCTACGGCTTCTTTCTCATGCTTGGCACCGTAGGTTTCCGCGCTGCTTTGCTGTTTGTTCGTCACATTTACAAGTCCATCAAGTGTGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002790 GO:0003674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007155 GO:0007165 GO:0008104 GO:0008150 GO:0008283 GO:0009306 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016192 GO:0022610 GO:0023052 GO:0030587 GO:0031224 GO:0031226 GO:0031589 GO:0032502 GO:0032940 GO:0033036 GO:0033299 GO:0034613 GO:0038023 GO:0042886 GO:0044425 GO:0044459 GO:0044464 GO:0045184 GO:0046903 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051703 GO:0051704 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072657 GO:0090702 GO:0098657 GO:0099120

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

593

Amino Acids

67.84

Weight (kDa)

7.84

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	53 - 549	6.6e-180	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005262)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14670 AT2G01970
fragaria_vesca	FvH4_4g35870 FvH4_4g35870 FvH4_4g35870 FvH4_4g35870 FvH4_4g35870
malus_domestica	MD13G1014300.v1.1 MD16G1011700.v1.1
prunus_persica	Prupe.1G339100_v2.0.a1
pyrus_communis	pycom13g01240
rosa_chinensis	RchiOBHm_Chr4g0445481
rosa_laevigata	RLG00000001580 RLG00000005738
rosa_multiflora	Rmu_sc0001509.1_g000009 Rmu_sc0010069.1_g000001
rosa_roxburghii	Rroxscaffold_3G00231310 Rroxscaffold_5G00385960
rosa_rugosa	Rorug04G0361500 Rorug04G0361600 Rorug07G0246700
rosa_samantha	Rh4AG423000 Rh4BG435000 Rh4CG449900 Rh4DG431300 Rh7AG400600 Rh7BG382100
rosa_wichuraiana	Rw4G036270 Rw7G033280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	264, 1502
AarI	CACCTGC	1 cut(s)	182
Acc16I	TGCGCA	1 cut(s)	336
Acc36I	ACCTGC	1 cut(s)	182
AccB1I	GGYRCC	2 cut(s)	1098, 1712
AccB7I	CCANNNNNTGG	1 cut(s)	1425
AccI	GTMKAC	1 cut(s)	549
AccII	CGCG	3 cut(s)	35, 47, 1728
AciI	CCGC	8 cut(s)	17, 35, 88, 522, 578, 1110, 1294, 1726
AclWI	GGATC	4 cut(s)	63, 1445, 1572, 1585
AcsI	RAATTY	3 cut(s)	700, 1277, 1317
AcuI	CTGAAG	2 cut(s)	782, 1557
AcyI	GRCGYC	2 cut(s)	75, 105
AfaI	GTAC	5 cut(s)	494, 590, 833, 1189, 1337
AfiI	CCNNNNNNNGG	4 cut(s)	34, 401, 727, 1425
AgsI	TTSAA	5 cut(s)	235, 515, 1061, 1283, 1400
AjiI	CACGTC	1 cut(s)	64
AluBI	AGCT	8 cut(s)	268, 316, 800, 907, 977, 1057, 1175, 1286
AluI	AGCT	8 cut(s)	268, 316, 800, 907, 977, 1057, 1175, 1286
Alw21I	GWGCWC	2 cut(s)	53, 259
Alw26I	GTCTC	1 cut(s)	812
Alw44I	GTGCAC	1 cut(s)	49
AlwI	GGATC	4 cut(s)	63, 1445, 1572, 1585
AlwNI	CAGNNNCTG	1 cut(s)	1425
Ama87I	CYCGRG	1 cut(s)	1311
AoxI	GGCC	4 cut(s)	134, 1112, 1585, 1677
ApaI	GGGCCC	2 cut(s)	138, 1116
ApaLI	GTGCAC	1 cut(s)	49
ApeKI	GCWGC	6 cut(s)	800, 881, 884, 1172, 1532, 1730
ApoI	RAATTY	3 cut(s)	700, 1277, 1317
Asp700I	GAANNNNTTC	1 cut(s)	770
AspLEI	GCGC	4 cut(s)	220, 337, 991, 1730
AspS9I	GGNCC	5 cut(s)	134, 135, 532, 1112, 1113
AsuC2I	CCSGG	2 cut(s)	1096, 1258
AsuHPI	GGTGA	5 cut(s)	206, 511, 854, 1087, 1221
AsuII	TTCGAA	1 cut(s)	343
AvaI	CYCGRG	1 cut(s)	1311
AvaII	GGWCC	1 cut(s)	532
BaeGI	GKGCMC	3 cut(s)	53, 138, 1116
BaeI	ACNNNNGTAYC	2 cut(s)	1179, 1212
BamHI	GGATCC	1 cut(s)	1577
BanI	GGYRCC	2 cut(s)	1098, 1712
BanII	GRGCYC	2 cut(s)	138, 1116
Bbv12I	GWGCWC	2 cut(s)	53, 259
BbvI	GCAGC	6 cut(s)	787, 868, 896, 1184, 1519, 1717
BccI	CCATC	6 cut(s)	404, 523, 594, 819, 1351, 1772
BceAI	ACGGC	2 cut(s)	92, 1708
BcgI	CGANNNNNNTGC	2 cut(s)	733, 767
BciVI	GTATCC	2 cut(s)	601, 1317
BclI	TGATCA	1 cut(s)	808
BcnI	CCSGG	2 cut(s)	1096, 1258
BcoDI	GTCTC	1 cut(s)	812
BfmI	CTRYAG	3 cut(s)	882, 1170, 1450
BfoI	RGCGCY	1 cut(s)	221
BfuAI	ACCTGC	1 cut(s)	182
BfuI	GTATCC	2 cut(s)	601, 1317
BisI	GCNGC	7 cut(s)	36, 801, 882, 885, 1173, 1533, 1731
BlpI	GCTNAGC	1 cut(s)	187
BlsI	GCNGC	7 cut(s)	37, 802, 883, 886, 1174, 1534, 1732
Bme1390I	CCNGG	2 cut(s)	1096, 1258
Bme18I	GGWCC	1 cut(s)	532
BmeT110I	CYCGRG	1 cut(s)	1311
BmgBI	CACGTC	1 cut(s)	64
BmgT120I	GGNCC	5 cut(s)	134, 135, 532, 1112, 1113
BmiI	GGNNCC	9 cut(s)	136, 534, 857, 901, 1100, 1114, 1115, 1579, 1714
BmrFI	CCNGG	2 cut(s)	1096, 1258
BmrI	ACTGGG	1 cut(s)	1081
BmsI	GCATC	3 cut(s)	475, 565, 1464
BmuI	ACTGGG	1 cut(s)	1081
Bpu1102I	GCTNAGC	1 cut(s)	187
Bpu14I	TTCGAA	1 cut(s)	343
BpuEI	CTTGAG	1 cut(s)	290
BpuMI	CCSGG	2 cut(s)	1096, 1258
BsaBI	GATNNNNATC	1 cut(s)	515
BsaHI	GRCGYC	2 cut(s)	75, 105
BsaJI	CCNNGG	5 cut(s)	321, 443, 889, 970, 1331
BsaWI	WCCGGW	1 cut(s)	852
Bsc4I	CCNNNNNNNGG	4 cut(s)	34, 401, 727, 1425
Bse1I	ACTGG	3 cut(s)	826, 1076, 1588
Bse3DI	GCAATG	3 cut(s)	1020, 1232, 1368
Bse8I	GATNNNNATC	1 cut(s)	515
BseDI	CCNNGG	5 cut(s)	321, 443, 889, 970, 1331
BseGI	GGATG	3 cut(s)	574, 830, 1455
BseJI	GATNNNNATC	1 cut(s)	515
BseLI	CCNNNNNNNGG	4 cut(s)	34, 401, 727, 1425
BseMI	GCAATG	3 cut(s)	1020, 1232, 1368
BseMII	CTCAG	3 cut(s)	201, 285, 1367
BseNI	ACTGG	3 cut(s)	826, 1076, 1588
BseRI	GAGGAG	1 cut(s)	987
BseSI	GKGCMC	3 cut(s)	53, 138, 1116
BseXI	GCAGC	6 cut(s)	787, 868, 896, 1184, 1519, 1717
BseYI	CCCAGC	2 cut(s)	149, 903
Bsh1236I	CGCG	3 cut(s)	35, 47, 1728
BshFI	GGCC	4 cut(s)	136, 1114, 1587, 1679
BshNI	GGYRCC	2 cut(s)	1098, 1712
BsiHKAI	GWGCWC	2 cut(s)	53, 259
BsiHKCI	CYCGRG	1 cut(s)	1311
BsiSI	CCGG	3 cut(s)	853, 1095, 1257
BslFI	GGGAC	1 cut(s)	95
BslI	CCNNNNNNNGG	4 cut(s)	34, 401, 727, 1425
BsmAI	GTCTC	1 cut(s)	812
BsmFI	GGGAC	1 cut(s)	95
BsnI	GGCC	4 cut(s)	136, 1114, 1587, 1679
BsoBI	CYCGRG	1 cut(s)	1311
Bsp119I	TTCGAA	1 cut(s)	343
Bsp120I	GGGCCC	2 cut(s)	134, 1112
Bsp1286I	GDGCHC	4 cut(s)	53, 138, 259, 1116
Bsp1407I	TGTACA	1 cut(s)	588
Bsp143I	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
Bsp1720I	GCTNAGC	1 cut(s)	187
Bsp19I	CCATGG	2 cut(s)	889, 1331
BspACI	CCGC	8 cut(s)	17, 35, 88, 522, 578, 1110, 1294, 1726
BspANI	GGCC	4 cut(s)	136, 1114, 1587, 1679
BspCNI	CTCAG	3 cut(s)	200, 286, 1366
BspFNI	CGCG	3 cut(s)	35, 47, 1728
BspHI	TCATGA	1 cut(s)	774
BspLI	GGNNCC	9 cut(s)	136, 534, 857, 901, 1100, 1114, 1115, 1579, 1714
BspMAI	CTGCAG	2 cut(s)	886, 1174
BspMI	ACCTGC	1 cut(s)	182
BspPI	GGATC	4 cut(s)	63, 1445, 1572, 1585
BspQI	GCTCTTC	1 cut(s)	1182
BspT104I	TTCGAA	1 cut(s)	343
BspT107I	GGYRCC	2 cut(s)	1098, 1712
BsrDI	GCAATG	3 cut(s)	1020, 1232, 1368
BsrGI	TGTACA	1 cut(s)	588
BsrI	ACTGG	3 cut(s)	826, 1076, 1588
BssECI	CCNNGG	5 cut(s)	321, 443, 889, 970, 1331
BssMI	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
BssNI	GRCGYC	2 cut(s)	75, 105
BssT1I	CCWWGG	4 cut(s)	321, 443, 889, 1331
Bst4CI	ACNGT	7 cut(s)	506, 593, 651, 715, 1147, 1507, 1717
Bst6I	CTCTTC	5 cut(s)	661, 810, 1182, 1332, 1595
BstACI	GRCGYC	2 cut(s)	75, 105
BstAUI	TGTACA	1 cut(s)	588
BstBI	TTCGAA	1 cut(s)	343
BstC8I	GCNNGC	5 cut(s)	49, 939, 1112, 1420, 1681
BstDEI	CTNAG	3 cut(s)	187, 294, 1353
BstDSI	CCRYGG	2 cut(s)	889, 1331
BstF5I	GGATG	3 cut(s)	574, 830, 1455
BstFNI	CGCG	3 cut(s)	35, 47, 1728
BstH2I	RGCGCY	1 cut(s)	221
BstHHI	GCGC	4 cut(s)	220, 337, 991, 1730
BstKTI	GATC	8 cut(s)	71, 175, 244, 811, 1440, 1543, 1580, 1631
BstMAI	GTCTC	1 cut(s)	812
BstMBI	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
BstMWI	GCNNNNNNNGC	4 cut(s)	44, 53, 1107, 1172
BstSCI	CCNGG	2 cut(s)	1094, 1256
BstSFI	CTRYAG	3 cut(s)	882, 1170, 1450
BstSLI	GKGCMC	3 cut(s)	53, 138, 1116
BstUI	CGCG	3 cut(s)	35, 47, 1728
BstV1I	GCAGC	6 cut(s)	787, 868, 896, 1184, 1519, 1717
BstX2I	RGATCY	3 cut(s)	68, 1437, 1577
BstXI	CCANNNNNNTGG	1 cut(s)	896
BstYI	RGATCY	3 cut(s)	68, 1437, 1577
BsuI	GTATCC	2 cut(s)	601, 1317
BsuRI	GGCC	4 cut(s)	136, 1114, 1587, 1679
BtgI	CCRYGG	2 cut(s)	889, 1331
BtrI	CACGTC	1 cut(s)	64
BtsCI	GGATG	3 cut(s)	574, 830, 1455
BtsI	GCAGTG	4 cut(s)	51, 659, 984, 1491
BtsIMutI	CAGTG	8 cut(s)	51, 598, 659, 984, 1143, 1390, 1491, 1581
BveI	ACCTGC	1 cut(s)	182
Cac8I	GCNNGC	5 cut(s)	49, 939, 1112, 1420, 1681
CaiI	CAGNNNCTG	1 cut(s)	1425
CciI	TCATGA	1 cut(s)	774
CfoI	GCGC	4 cut(s)	220, 337, 991, 1730
Cfr13I	GGNCC	5 cut(s)	134, 135, 532, 1112, 1113
CseI	GACGC	4 cut(s)	83, 113, 697, 1411
Csp6I	GTAC	5 cut(s)	493, 589, 832, 1188, 1336
CspCI	CAANNNNNGTGG	2 cut(s)	654, 689
CviQI	GTAC	5 cut(s)	493, 589, 832, 1188, 1336
DdeI	CTNAG	3 cut(s)	187, 294, 1353
DpnI	GATC	8 cut(s)	70, 174, 243, 810, 1439, 1542, 1579, 1630
DpnII	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
Eam1104I	CTCTTC	5 cut(s)	661, 810, 1182, 1332, 1595
EarI	CTCTTC	5 cut(s)	661, 810, 1182, 1332, 1595
Eco130I	CCWWGG	4 cut(s)	321, 443, 889, 1331
Eco24I	GRGCYC	2 cut(s)	138, 1116
Eco47I	GGWCC	1 cut(s)	532
Eco57I	CTGAAG	2 cut(s)	782, 1557
Eco88I	CYCGRG	1 cut(s)	1311
EcoO109I	RGGNCCY	2 cut(s)	135, 1113
EcoT14I	CCWWGG	4 cut(s)	321, 443, 889, 1331
EcoT38I	GRGCYC	2 cut(s)	138, 1116
ErhI	CCWWGG	4 cut(s)	321, 443, 889, 1331
FaqI	GGGAC	1 cut(s)	95
FauI	CCCGC	2 cut(s)	24, 1103
FbaI	TGATCA	1 cut(s)	808
FblI	GTMKAC	1 cut(s)	549
Fnu4HI	GCNGC	7 cut(s)	36, 801, 882, 885, 1173, 1533, 1731
FokI	GGATG	3 cut(s)	581, 837, 1442
FriOI	GRGCYC	2 cut(s)	138, 1116
Fsp4HI	GCNGC	7 cut(s)	36, 801, 882, 885, 1173, 1533, 1731
FspI	TGCGCA	1 cut(s)	336
GlaI	GCGC	4 cut(s)	219, 336, 990, 1729
GluI	GCNGC	7 cut(s)	36, 801, 882, 885, 1173, 1533, 1731
GsaI	CCCAGC	2 cut(s)	153, 907
HaeII	RGCGCY	1 cut(s)	221
HaeIII	GGCC	4 cut(s)	136, 1114, 1587, 1679
HapII	CCGG	3 cut(s)	853, 1095, 1257
HgaI	GACGC	4 cut(s)	83, 113, 697, 1411
HhaI	GCGC	4 cut(s)	220, 337, 991, 1730
Hin1I	GRCGYC	2 cut(s)	75, 105
Hin6I	GCGC	4 cut(s)	218, 335, 989, 1728
HinP1I	GCGC	4 cut(s)	218, 335, 989, 1728
HincII	GTYRAC	1 cut(s)	421
HindII	GTYRAC	1 cut(s)	421
HindIII	AAGCTT	1 cut(s)	266
HinfI	GANTC	3 cut(s)	290, 745, 756
HpaII	CCGG	3 cut(s)	853, 1095, 1257
HphI	GGTGA	5 cut(s)	206, 511, 854, 1087, 1221
Hpy166II	GTNNAC	4 cut(s)	51, 421, 550, 912
Hpy188I	TCNGA	7 cut(s)	68, 73, 82, 295, 607, 1356, 1633
Hpy188III	TCNNGA	6 cut(s)	760, 775, 812, 897, 1123, 1313
Hpy8I	GTNNAC	4 cut(s)	51, 421, 550, 912
Hpy99I	CGWCG	2 cut(s)	77, 107
HpyAV	CCTTC	7 cut(s)	92, 148, 190, 196, 303, 422, 1055
HpyCH4III	ACNGT	7 cut(s)	506, 593, 651, 715, 1147, 1507, 1717
HpyCH4IV	ACGT	2 cut(s)	63, 158
HpyF10VI	GCNNNNNNNGC	4 cut(s)	44, 53, 1107, 1172
HpyF3I	CTNAG	3 cut(s)	187, 294, 1353
HpySE526I	ACGT	2 cut(s)	63, 158
Hsp92I	GRCGYC	2 cut(s)	75, 105
HspAI	GCGC	4 cut(s)	218, 335, 989, 1728
Ksp22I	TGATCA	1 cut(s)	808
Kzo9I	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
LguI	GCTCTTC	1 cut(s)	1182
LmnI	GCTCC	3 cut(s)	262, 974, 1291
Lsp1109I	GCAGC	6 cut(s)	787, 868, 896, 1184, 1519, 1717
LweI	GCATC	3 cut(s)	475, 565, 1464
MaeII	ACGT	2 cut(s)	63, 158
MaeIII	GTNAC	8 cut(s)	709, 1086, 1222, 1430, 1489, 1601, 1670, 1748
MalI	GATC	8 cut(s)	70, 174, 243, 810, 1439, 1542, 1579, 1630
MboI	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
MfeI	CAATTG	1 cut(s)	1152
MflI	RGATCY	3 cut(s)	68, 1437, 1577
MhlI	GDGCHC	4 cut(s)	53, 138, 259, 1116
MluCI	AATT	6 cut(s)	479, 700, 1152, 1200, 1277, 1317
MlyI	GAGTC	2 cut(s)	284, 765
MmeI	TCCRAC	5 cut(s)	96, 105, 585, 1346, 1549
MroXI	GAANNNNTTC	1 cut(s)	770
MseI	TTAA	1 cut(s)	1140
MslI	CAYNNNNRTG	2 cut(s)	843, 932
MspA1I	CMGCKG	1 cut(s)	907
MspI	CCGG	3 cut(s)	853, 1095, 1257
MspR9I	CCNGG	2 cut(s)	1096, 1258
MunI	CAATTG	1 cut(s)	1152
MvnI	CGCG	3 cut(s)	35, 47, 1728
MwoI	GCNNNNNNNGC	4 cut(s)	44, 53, 1107, 1172
NciI	CCSGG	2 cut(s)	1096, 1258
NcoI	CCATGG	2 cut(s)	889, 1331
NdeII	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
NlaIV	GGNNCC	9 cut(s)	136, 534, 857, 901, 1100, 1114, 1115, 1579, 1714
NmuCI	GTSAC	4 cut(s)	709, 1430, 1489, 1748
NsbI	TGCGCA	1 cut(s)	336
NspV	TTCGAA	1 cut(s)	343
PaeR7I	CTCGAG	1 cut(s)	1311
PagI	TCATGA	1 cut(s)	774
PaqCI	CACCTGC	1 cut(s)	182
PciSI	GCTCTTC	1 cut(s)	1182
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PdmI	GAANNNNTTC	1 cut(s)	770
PfeI	GAWTC	1 cut(s)	745
PflFI	GACNNNGTC	1 cut(s)	107
PflMI	CCANNNNNTGG	1 cut(s)	1425
PkrI	GCNGC	7 cut(s)	37, 802, 883, 886, 1174, 1534, 1732
PleI	GAGTC	2 cut(s)	284, 764
PpsI	GAGTC	2 cut(s)	284, 764
PsiI	TTATAA	2 cut(s)	264, 1502
PspFI	CCCAGC	2 cut(s)	149, 903
PspN4I	GGNNCC	9 cut(s)	136, 534, 857, 901, 1100, 1114, 1115, 1579, 1714
PspOMI	GGGCCC	2 cut(s)	134, 1112
PspPI	GGNCC	5 cut(s)	134, 135, 532, 1112, 1113
PstI	CTGCAG	2 cut(s)	886, 1174
PstNI	CAGNNNCTG	1 cut(s)	1425
PsuI	RGATCY	3 cut(s)	68, 1437, 1577
PsyI	GACNNNGTC	1 cut(s)	107
PvuII	CAGCTG	1 cut(s)	907
RsaI	GTAC	5 cut(s)	494, 590, 833, 1189, 1337
RsaNI	GTAC	5 cut(s)	493, 589, 832, 1188, 1336
RseI	CAYNNNNRTG	2 cut(s)	843, 932
SapI	GCTCTTC	1 cut(s)	1182
SaqAI	TTAA	1 cut(s)	1140
SatI	GCNGC	7 cut(s)	36, 801, 882, 885, 1173, 1533, 1731
Sau3AI	GATC	8 cut(s)	68, 172, 241, 808, 1437, 1540, 1577, 1628
Sau96I	GGNCC	5 cut(s)	134, 135, 532, 1112, 1113
SchI	GAGTC	2 cut(s)	284, 765
ScrFI	CCNGG	2 cut(s)	1096, 1258
SduI	GDGCHC	4 cut(s)	53, 138, 259, 1116
SfaNI	GCATC	3 cut(s)	475, 565, 1464
SfcI	CTRYAG	3 cut(s)	882, 1170, 1450
Sfr274I	CTCGAG	1 cut(s)	1311
SfuI	TTCGAA	1 cut(s)	343
SinI	GGWCC	1 cut(s)	532
SlaI	CTCGAG	1 cut(s)	1311
SmiMI	CAYNNNNRTG	2 cut(s)	843, 932
SmlI	CTYRAG	2 cut(s)	269, 1311
SmoI	CTYRAG	2 cut(s)	269, 1311
Sse9I	AATT	6 cut(s)	479, 700, 1152, 1200, 1277, 1317
SsiI	CCGC	8 cut(s)	17, 35, 88, 522, 578, 1110, 1294, 1726
SspI	AATATT	2 cut(s)	458, 924
StyD4I	CCNGG	2 cut(s)	1094, 1256
StyI	CCWWGG	4 cut(s)	321, 443, 889, 1331
TaaI	ACNGT	7 cut(s)	506, 593, 651, 715, 1147, 1507, 1717
TaiI	ACGT	2 cut(s)	66, 161
TaqI	TCGA	2 cut(s)	343, 1312
TasI	AATT	6 cut(s)	479, 700, 1152, 1200, 1277, 1317
TatI	WGTACW	2 cut(s)	588, 831
TauI	GCSGC	1 cut(s)	38
TfiI	GAWTC	1 cut(s)	745
Tru1I	TTAA	1 cut(s)	1140
Tru9I	TTAA	1 cut(s)	1140
TscAI	CASTG	8 cut(s)	58, 598, 666, 991, 1150, 1390, 1498, 1588
TseFI	GTSAC	4 cut(s)	709, 1430, 1489, 1748
TseI	GCWGC	6 cut(s)	800, 881, 884, 1172, 1532, 1730
Tsp45I	GTSAC	4 cut(s)	709, 1430, 1489, 1748
TspGWI	ACGGA	1 cut(s)	572
TspRI	CASTG	8 cut(s)	58, 598, 666, 991, 1150, 1390, 1498, 1588
Tth111I	GACNNNGTC	1 cut(s)	107
Van91I	CCANNNNNTGG	1 cut(s)	1425
VneI	GTGCAC	1 cut(s)	49
VpaK11BI	GGWCC	1 cut(s)	532
XapI	RAATTY	3 cut(s)	700, 1277, 1317
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1329, 1354
XhoI	CTCGAG	1 cut(s)	1311
XmiI	GTMKAC	1 cut(s)	549
XmnI	GAANNNNTTC	1 cut(s)	770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.