MD16G1110600.v1.1

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017288
Physical Location & Seq
Forward (+)
7781960 .. 7783273
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1110600.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGGGGCCATGGAAGTTCAAAACATATCCAGAGAAGTCATCAAACCTTCCACTCCAACCCCACCACATCTTCGAACACACAAACTCTCGATCCTCGATCAGATTAACCCTCGCTCTTATGTTCCGGTCGTTTACTTCTACCCCAAGGAAGCCTCCGATTATTGGTCTGTCGCAGATAAATCCAATCAGCTCAAGAAATCACTATCCGAAACACTGTCCAAATACTACCCTTTTGCCGGCAGGATCAGAGACAGATTCTCCATCGACTGCAACGATGAAGGAGTCGCATTTGTTGAAACGCAAATCAGAAACTTCAAGCTCTCTGACATTCTTGAGAACCCGAAAGACGAGGACCTGGCGTTGTTAATCTCCGAAGATGTGGCGTGGAATGAGGATCCGGATGTCAGTGTGATCGTGGATGTTCAAGTGAGCTTCTTTGAGTGCGGTGGAATGGCACTTGGCATTGGTATGTCACACAAGATAGCTGATGCATCCACCATAATCAATTTCGTTAACGACTGGGCAGCTGTGGCTCGTGATCAGTATGTACCATCCCCTGAGTTCGTCGCGGAAAATATATTTCCCCAAGGTGACCTGCCACTGTTCCCAGAAGCTGTGCCCGAGAAAAGCGATTGTGTCGCGAAAAGATTTGTGTTTGATGCCACAAAGATTGCTGCCCTCAAAGCCTTAGTCGCGGATAAAGTGCAAAACCCCACGAGGGTTGAAGTTGTCGCAGCATTTATTTATAGCTGCACAATTTCTGCGCTAAGATTGACTTCAGGGTCTTCCTCAAACCCGACTGTTCTCACTCAATCAGTGAACCTGCGCACTAGAATGGTTCCTCCGGTACCGCAAAACTCTGTAGGTTGCATGGTTTGGATATATACAGTGTCCACAGCTGAGGACAGCGTGATTGAGTTGCATGACCTGGTGGCTCAGCTGAAGCAGAATATGACCCGATTTTGTGATTCATATGCTCAAAAGTTTCGCGGGAAAGATCAATGGCCGGTGATCTTCCAAGAGTGTTTGCAAGAATCAATGGTTGAATTTTCGAGACCAAATTTGGTGATGTATAAATGTACAAGCTGGTGCAGGTTCCCAATGTATGAAGTTGACTTTGGTTGGGGAAAGCCAATTTGGATCACCGTTCCTAGCTGCACGACTAAGGACTGCTTTATTCTGATTGATACGAAAAATGGAGAAGGGATTGAAGCATATGTGAATTTGGAAAAGCAGCAACTGGATGTGTTTGAGCGCGACGAGGAGCTTCTTGCATTTGCTTCTTTGAATCCAAGCATTTCAGACTCCGTTTGA

Protein Analysis

438

Amino Acids

49.18

Weight (kDa)

4.8

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 423 1.8e-84 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017288)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24550
malus_domestica MD16G1110600.v1.1 MD16G1110700.v1.1
pyrus_communis pycom16g09390
rosa_chinensis RchiOBHm_Chr4g0431311 RchiOBHm_Chr4g0431461
rosa_laevigata RLG00000006912
rosa_multiflora Rmu_sc0007885.1_g000012
rosa_roxburghii Rroxscaffold_5G00372910
rosa_rugosa Rorug04G0250400
rosa_samantha Rh4AG306500 Rh4DG309700
rosa_wichuraiana Rw4G026600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 781
Acc16I TGCGCA 1 cut(s) 827
Acc36I ACCTGC 3 cut(s) 603, 831, 1083
Acc65I GGTACC 1 cut(s) 847
AccB1I GGYRCC 1 cut(s) 847
AccII CGCG 5 cut(s) 569, 641, 695, 990, 1257
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 5 cut(s) 444, 569, 695, 851, 990
AclWI GGATC 5 cut(s) 85, 251, 389, 402, 1148
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 3 cut(s) 1046, 1060, 1222
AcuI CTGAAG 2 cut(s) 762, 962
AfaI GTAC 3 cut(s) 549, 849, 1081
AfiI CCNNNNNNNGG 3 cut(s) 162, 236, 718
AgsI TTSAA 8 cut(s) 20, 296, 316, 425, 725, 1046, 1211, 1288
AjnI CCWGG 2 cut(s) 354, 927
AjuI GAANNNNNNNTTGG 4 cut(s) 1101, 1120, 1133, 1152
Alw26I GTCTC 2 cut(s) 243, 1048
AlwI GGATC 5 cut(s) 85, 251, 389, 402, 1148
AlwNI CAGNNNCTG 2 cut(s) 614, 1240
Ama87I CYCGRG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 2 cut(s) 6, 1004
ApeKI GCWGC 6 cut(s) 524, 674, 734, 750, 1155, 1234
ApoI RAATTY 3 cut(s) 1046, 1060, 1222
ArsI GACNNNNNNTTYG 2 cut(s) 675, 707
Asp718I GGTACC 1 cut(s) 847
AspLEI GCGC 3 cut(s) 766, 828, 1257
AspS9I GGNCC 2 cut(s) 6, 352
AsuHPI GGTGA 4 cut(s) 602, 1021, 1078, 1135
AsuII TTCGAA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 621
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 847
BauI CACGAG 2 cut(s) 534, 715
BbsI GAAGAC 1 cut(s) 777
BbvCI CCTCAGC 1 cut(s) 900
BbvI GCAGC 6 cut(s) 536, 661, 737, 746, 1142, 1246
BccI CCATC 2 cut(s) 269, 559
BciT130I CCWGG 2 cut(s) 356, 929
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 2 cut(s) 243, 1048
BfaI CTAG 2 cut(s) 831, 1152
BfmI CTRYAG 1 cut(s) 861
BfuAI ACCTGC 3 cut(s) 603, 831, 1083
BisI GCNGC 6 cut(s) 525, 675, 735, 751, 1156, 1235
BlpI GCTNAGC 1 cut(s) 936
BlsI GCNGC 6 cut(s) 526, 676, 736, 752, 1157, 1236
Bme1390I CCNGG 2 cut(s) 356, 929
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 2 cut(s) 6, 352
BmiI GGNNCC 5 cut(s) 7, 396, 840, 849, 1097
BmrFI CCNGG 2 cut(s) 356, 929
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 478, 500, 649
BmuI ACTGGG 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 777
Bpu10I CCTNAGC 1 cut(s) 900
Bpu1102I GCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 2 cut(s) 176, 353
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 3 cut(s) 9, 144, 586
BsaWI WCCGGW 3 cut(s) 124, 397, 844
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 236, 718
Bse118I RCCGGY 2 cut(s) 236, 1006
Bse1I ACTGG 2 cut(s) 524, 1245
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 2 cut(s) 356, 929
BseDI CCNNGG 3 cut(s) 9, 144, 586
BseGI GGATG 5 cut(s) 406, 424, 491, 551, 1249
BseLI CCNNNNNNNGG 3 cut(s) 162, 236, 718
BseMII CTCAG 3 cut(s) 549, 891, 950
BseNI ACTGG 2 cut(s) 524, 1245
BseRI GAGGAG 1 cut(s) 1277
BseSI GKGCMC 1 cut(s) 621
BseXI GCAGC 6 cut(s) 536, 661, 737, 746, 1142, 1246
BsgI GTGCAG 3 cut(s) 736, 1111, 1141
Bsh1236I CGCG 5 cut(s) 569, 641, 695, 990, 1257
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 8, 1006
BshNI GGYRCC 1 cut(s) 847
BsiEI CGRYCG 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 5 cut(s) 125, 237, 398, 845, 1007
BslI CCNNNNNNNGG 3 cut(s) 162, 236, 718
BsmAI GTCTC 2 cut(s) 243, 1048
BsnI GGCC 2 cut(s) 8, 1006
Bso31I GGTCTC 1 cut(s) 1048
BsoBI CYCGRG 1 cut(s) 620
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 621
Bsp13I TCCGGA 1 cut(s) 397
Bsp1407I TGTACA 1 cut(s) 1079
Bsp143I GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
Bsp1720I GCTNAGC 1 cut(s) 936
Bsp19I CCATGG 1 cut(s) 9
Bsp68I TCGCGA 1 cut(s) 641
BspACI CCGC 5 cut(s) 444, 569, 695, 851, 990
BspANI GGCC 2 cut(s) 8, 1006
BspCNI CTCAG 3 cut(s) 550, 892, 949
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 5 cut(s) 569, 641, 695, 990, 1257
BspLI GGNNCC 5 cut(s) 7, 396, 840, 849, 1097
BspMI ACCTGC 3 cut(s) 603, 831, 1083
BspPI GGATC 5 cut(s) 85, 251, 389, 402, 1148
BspT104I TTCGAA 1 cut(s) 73
BspT107I GGYRCC 1 cut(s) 847
BspTNI GGTCTC 1 cut(s) 1048
BsrFI RCCGGY 2 cut(s) 236, 1006
BsrGI TGTACA 1 cut(s) 1079
BsrI ACTGG 2 cut(s) 524, 1245
BssAI RCCGGY 2 cut(s) 236, 1006
BssECI CCNNGG 3 cut(s) 9, 144, 586
BssMI GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
BssSI CACGAG 2 cut(s) 534, 715
BssT1I CCWWGG 3 cut(s) 9, 144, 586
Bst2BI CACGAG 2 cut(s) 534, 715
Bst2UI CCWGG 2 cut(s) 356, 929
Bst4CI ACNGT 5 cut(s) 216, 603, 802, 889, 1147
BstAUI TGTACA 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 6 cut(s) 558, 688, 767, 900, 936, 1164
BstDSI CCRYGG 1 cut(s) 9
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 5 cut(s) 406, 424, 491, 551, 1249
BstFNI CGCG 5 cut(s) 569, 641, 695, 990, 1257
BstHHI GCGC 3 cut(s) 766, 828, 1257
BstKTI GATC 9 cut(s) 93, 100, 246, 397, 414, 541, 1000, 1014, 1143
BstMAI GTCTC 2 cut(s) 243, 1048
BstMBI GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 530, 683, 692
BstNI CCWGG 2 cut(s) 356, 929
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 354, 927
BstSFI CTRYAG 1 cut(s) 861
BstSLI GKGCMC 1 cut(s) 621
BstUI CGCG 5 cut(s) 569, 641, 695, 990, 1257
BstV1I GCAGC 6 cut(s) 536, 661, 737, 746, 1142, 1246
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 2 cut(s) 8, 1006
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 406, 424, 491, 551, 1249
BtsIMutI CAGTG 5 cut(s) 212, 412, 599, 822, 894
BtuMI TCGCGA 1 cut(s) 641
BveI ACCTGC 3 cut(s) 603, 831, 1083
Cac8I GCNNGC 1 cut(s) 238
CaiI CAGNNNCTG 2 cut(s) 614, 1240
CfoI GCGC 3 cut(s) 766, 828, 1257
Cfr10I RCCGGY 2 cut(s) 236, 1006
Cfr13I GGNCC 2 cut(s) 6, 352
CsiI ACCWGGT 1 cut(s) 927
Csp6I GTAC 3 cut(s) 548, 848, 1080
CspCI CAANNNNNGTGG 4 cut(s) 652, 687, 703, 738
CviAII CATG 3 cut(s) 10, 871, 923
CviQI GTAC 3 cut(s) 548, 848, 1080
DdeI CTNAG 6 cut(s) 558, 688, 767, 900, 936, 1164
DpnI GATC 9 cut(s) 92, 99, 245, 396, 413, 540, 999, 1013, 1142
DpnII GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
DrdI GACNNNNNNGTC 1 cut(s) 781
DseDI GACNNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 1 cut(s) 1004
Eco130I CCWWGG 3 cut(s) 9, 144, 586
Eco31I GGTCTC 1 cut(s) 1048
Eco47I GGWCC 1 cut(s) 352
Eco57I CTGAAG 2 cut(s) 762, 962
Eco88I CYCGRG 1 cut(s) 620
Eco91I GGTNACC 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 352
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 354, 927
EcoT14I CCWWGG 3 cut(s) 9, 144, 586
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 3 cut(s) 9, 144, 586
FaeI CATG 3 cut(s) 13, 874, 926
FalI AAGNNNNNCTT 4 cut(s) 760, 792, 1157, 1189
FatI CATG 3 cut(s) 9, 870, 922
FauI CCCGC 1 cut(s) 983
FauNDI CATATG 2 cut(s) 973, 1216
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 6 cut(s) 525, 675, 735, 751, 1156, 1235
FokI GGATG 5 cut(s) 413, 431, 478, 538, 1256
Fsp4HI GCNGC 6 cut(s) 525, 675, 735, 751, 1156, 1235
FspBI CTAG 2 cut(s) 831, 1152
FspI TGCGCA 1 cut(s) 827
GlaI GCGC 3 cut(s) 765, 827, 1256
GluI GCNGC 6 cut(s) 525, 675, 735, 751, 1156, 1235
HaeIII GGCC 2 cut(s) 8, 1006
HapII CCGG 5 cut(s) 125, 237, 398, 845, 1007
HhaI GCGC 3 cut(s) 766, 828, 1257
Hin1II CATG 3 cut(s) 13, 874, 926
Hin6I GCGC 3 cut(s) 764, 826, 1255
HinP1I GCGC 3 cut(s) 764, 826, 1255
HincII GTYRAC 2 cut(s) 514, 1114
HindII GTYRAC 2 cut(s) 514, 1114
HinfI GANTC 6 cut(s) 255, 282, 968, 1034, 1288, 1304
HpaI GTTAAC 1 cut(s) 514
HpaII CCGG 5 cut(s) 125, 237, 398, 845, 1007
HphI GGTGA 4 cut(s) 602, 1021, 1078, 1135
Hpy166II GTNNAC 5 cut(s) 133, 514, 820, 894, 1114
Hpy188I TCNGA 9 cut(s) 102, 157, 208, 248, 308, 325, 373, 1182, 1303
Hpy188III TCNNGA 8 cut(s) 30, 88, 193, 332, 398, 536, 640, 1053
Hpy8I GTNNAC 5 cut(s) 133, 514, 820, 894, 1114
Hpy99I CGWCG 2 cut(s) 569, 1262
HpyAV CCTTC 3 cut(s) 57, 272, 1196
HpyCH4III ACNGT 5 cut(s) 216, 603, 802, 889, 1147
HpyF10VI GCNNNNNNNGC 3 cut(s) 530, 683, 692
HpyF3I CTNAG 6 cut(s) 558, 688, 767, 900, 936, 1164
Hsp92II CATG 3 cut(s) 13, 874, 926
HspAI GCGC 3 cut(s) 764, 826, 1255
Kpn2I TCCGGA 1 cut(s) 397
KpnI GGTACC 1 cut(s) 851
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 538
KspAI GTTAAC 1 cut(s) 514
Kzo9I GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
LmnI GCTCC 1 cut(s) 1264
Lsp1109I GCAGC 6 cut(s) 536, 661, 737, 746, 1142, 1246
LweI GCATC 3 cut(s) 478, 500, 649
MabI ACCWGGT 1 cut(s) 927
MaeI CTAG 2 cut(s) 831, 1152
MaeIII GTNAC 2 cut(s) 471, 590
MalI GATC 9 cut(s) 92, 99, 245, 396, 413, 540, 999, 1013, 1142
MboI GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
MboII GAAGA 4 cut(s) 62, 386, 777, 1006
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 6 cut(s) 505, 756, 1046, 1060, 1134, 1222
MlyI GAGTC 2 cut(s) 291, 1298
MmeI TCCRAC 1 cut(s) 80
Mph1103I ATGCAT 1 cut(s) 493
MroI TCCGGA 1 cut(s) 397
MroNI GCCGGC 1 cut(s) 236
MseI TTAA 3 cut(s) 105, 365, 513
MslI CAYNNNNRTG 2 cut(s) 467, 833
MspA1I CMGCKG 3 cut(s) 527, 899, 940
MspI CCGG 5 cut(s) 125, 237, 398, 845, 1007
MspR9I CCNGG 2 cut(s) 356, 929
MvaI CCWGG 2 cut(s) 356, 929
MvnI CGCG 5 cut(s) 569, 641, 695, 990, 1257
MwoI GCNNNNNNNGC 3 cut(s) 530, 683, 692
NaeI GCCGGC 1 cut(s) 238
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 2 cut(s) 973, 1216
NdeII GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 3 cut(s) 13, 874, 926
NlaIV GGNNCC 5 cut(s) 7, 396, 840, 849, 1097
NmuCI GTSAC 2 cut(s) 471, 590
NruI TCGCGA 1 cut(s) 641
NsbI TGCGCA 1 cut(s) 827
NsiI ATGCAT 1 cut(s) 493
NspV TTCGAA 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 4 cut(s) 255, 968, 1034, 1288
PkrI GCNGC 6 cut(s) 526, 676, 736, 752, 1157, 1236
PleI GAGTC 2 cut(s) 290, 1298
PpsI GAGTC 2 cut(s) 290, 1298
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 354, 927
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 2 cut(s) 354, 927
PspN4I GGNNCC 5 cut(s) 7, 396, 840, 849, 1097
PspPI GGNCC 2 cut(s) 6, 352
PspPPI RGGWCCY 1 cut(s) 352
PstNI CAGNNNCTG 2 cut(s) 614, 1240
PsuI RGATCY 1 cut(s) 394
PvuII CAGCTG 3 cut(s) 527, 899, 940
RruI TCGCGA 1 cut(s) 641
RsaI GTAC 3 cut(s) 549, 849, 1081
RsaNI GTAC 3 cut(s) 548, 848, 1080
RseI CAYNNNNRTG 2 cut(s) 467, 833
SaqAI TTAA 3 cut(s) 105, 365, 513
SatI GCNGC 6 cut(s) 525, 675, 735, 751, 1156, 1235
Sau3AI GATC 9 cut(s) 90, 97, 243, 394, 411, 538, 997, 1011, 1140
Sau96I GGNCC 2 cut(s) 6, 352
SchI GAGTC 2 cut(s) 291, 1298
ScrFI CCNGG 2 cut(s) 356, 929
SduI GDGCHC 1 cut(s) 621
SexAI ACCWGGT 1 cut(s) 927
SfaNI GCATC 3 cut(s) 478, 500, 649
SfcI CTRYAG 1 cut(s) 861
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 467, 833
SmlI CTYRAG 2 cut(s) 191, 332
SmoI CTYRAG 2 cut(s) 191, 332
Sse9I AATT 6 cut(s) 505, 756, 1046, 1060, 1134, 1222
SsiI CCGC 5 cut(s) 444, 569, 695, 851, 990
SspMI CTAG 2 cut(s) 831, 1152
StyD4I CCNGG 2 cut(s) 354, 927
StyI CCWWGG 3 cut(s) 9, 144, 586
TaaI ACNGT 5 cut(s) 216, 603, 802, 889, 1147
TaqI TCGA 5 cut(s) 73, 89, 96, 264, 1052
TasI AATT 6 cut(s) 505, 756, 1046, 1060, 1134, 1222
TatI WGTACW 1 cut(s) 1079
TfiI GAWTC 4 cut(s) 255, 968, 1034, 1288
Tru1I TTAA 3 cut(s) 105, 365, 513
Tru9I TTAA 3 cut(s) 105, 365, 513
TscAI CASTG 5 cut(s) 219, 412, 606, 822, 894
TseFI GTSAC 2 cut(s) 471, 590
TseI GCWGC 6 cut(s) 524, 674, 734, 750, 1155, 1234
Tsp45I GTSAC 2 cut(s) 471, 590
TspDTI ATGAA 3 cut(s) 291, 960, 1122
TspGWI ACGGA 1 cut(s) 1297
TspRI CASTG 5 cut(s) 219, 412, 606, 822, 894
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 3 cut(s) 1046, 1060, 1222
XspI CTAG 2 cut(s) 831, 1152
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.