Rh4AG306500

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017288
Physical Location & Seq
Reverse (-)
61842808 .. 61844142
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG306500.1

Sequence Viewer

Length: 1335 bp
ATGACAAGCATGGAAGTTGAAATAATATCCAGAGAAACCATCAAACCTTCCACTCCAACCCTACCACATCACGGAACATACCACCTTTCTTTCCTTGAACAGATAAATCCTCGTACCTATACTCCAGTTGTTTACTTCTACCCCAAGGAAGAATCTGATTCTGATCATAATCCTAATTTTGGTGATAAATCCAATCAGCTCAAGAAATCTCTATCAGAAACACTAGCCAAATACTACCCATTTTCCGGTAGGATCAGAGATAGAGTCTCCATTGATTGTAATGATGAAGGAGTCATATTTGTAGAAGCAAGAATCAAAGTCAAGCTCTCTGAAATTCTCGAGCACCCGAAAGATGAGGTGCTGGACCTGTTATTCACAGACAATTTACAGTGGAACGATGACTCCAATTTAACTGTCATCTTGGCTGTTCAAGTAAGCTTCTTTGACTGTGGTGGAATGGCAATTGGCATCTGTATGTCACACAAAGTAGCTGACACTTCCACCATGATCAACTTCATCAACGACTGGGCAGCCATAACCCGTGATCACAAAGCTGATCAATATGTGTCTCCCGAGTTTATTGCAGCAACTGTATTCCCCCAAGGTGAGATTCCACTGCTACCACAATCTGTGATTGATAAAGGGGATTACGTTTCCAAGAGATTCGTGTTTGCTGCCCCGAAAGTTGCTGCCCTCAAAGCCATGGTGTGTGACAAAGTGCAAAACCCCTCAAAAGTTGAAGTTGTGACTGCTTTTATTCATAGATGTGCCATATCTGCATTGAAATCGAATTCTGGGTCCTTGTTGAATCCTAGTACCCTTTTGATCCAGACGGTGAATTTGCGTGGTAGAATGGTTCCTCCATTGCCGAAAAGCTCAGTAGGTAGCATGGTTTGGTTCTATGCAGTGCCCACAGCGGAGGATGAGAGTGTGATAGAGTTGCATGACCTGGTGTCTCAAATGAAGGAGAGGATGGCCCATTTCTGTGTTACATATGCCGAGAAATTTGGAAAAGAATGGCCTTTGATATTTATGGAGTGCATGAAAGATTCAAGGAAACTGTTTCAAAAACAAAACCTGGTTGTGTATAGATGTAGCAGTTGGTGCAGATTCCCAGTTTATGAAGCAGACTTTGGTTGGGGCAAGCCGATTTGGGTCACTATTGCTAGCTGTGTGATGAAAAATAGTATCTTTCTGATTGATACTAGGAATGGAGAAGGGATTGAAGCACATGTGAATTTGGAAAAGCAACACATGGATGTGTTTGAGCGTGATGCAGAGCTTCTTGCATTTGCTTCTCTGAATCCCAGTGCTAATATAGACAACAATATGTGA

Protein Analysis

444

Amino Acids

50.2

Weight (kDa)

5.69

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 4.1e-88 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017288)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24550
malus_domestica MD16G1110600.v1.1 MD16G1110700.v1.1
pyrus_communis pycom16g09390
rosa_chinensis RchiOBHm_Chr4g0431311 RchiOBHm_Chr4g0431461
rosa_laevigata RLG00000006912
rosa_multiflora Rmu_sc0007885.1_g000012
rosa_roxburghii Rroxscaffold_5G00372910
rosa_rugosa Rorug04G0250400
rosa_samantha Rh4AG306500 Rh4DG309700
rosa_wichuraiana Rw4G026600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 917
AclWI GGATC 2 cut(s) 260, 820
AcsI RAATTY 5 cut(s) 333, 790, 838, 1004, 1237
AfaI GTAC 2 cut(s) 115, 817
AfiI CCNNNNNNNGG 3 cut(s) 71, 179, 245
AflIII ACRYGT 1 cut(s) 1231
AgsI TTSAA 9 cut(s) 20, 98, 431, 740, 784, 808, 1053, 1067, 1226
AhdI GACNNNNNGTC 1 cut(s) 952
AjnI CCWGG 2 cut(s) 948, 1077
AjuI GAANNNNNNNTTGG 4 cut(s) 594, 626, 1116, 1148
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
AluBI AGCT 8 cut(s) 199, 325, 438, 491, 554, 876, 1170, 1282
AluI AGCT 8 cut(s) 199, 325, 438, 491, 554, 876, 1170, 1282
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 3 cut(s) 271, 573, 960
AlwI GGATC 2 cut(s) 260, 820
AlwNI CAGNNNCTG 1 cut(s) 590
Ama87I CYCGRG 2 cut(s) 338, 572
AoxI GGCC 2 cut(s) 975, 1019
ApeKI GCWGC 4 cut(s) 530, 584, 674, 689
ApoI RAATTY 5 cut(s) 333, 790, 838, 1004, 1237
ArsI GACNNNNNNTTYG 4 cut(s) 782, 814, 823, 855
AspS9I GGNCC 3 cut(s) 364, 798, 976
AsuHPI GGTGA 3 cut(s) 194, 617, 847
AsuNHI GCTAGC 1 cut(s) 1166
AvaI CYCGRG 2 cut(s) 338, 572
AvaII GGWCC 2 cut(s) 364, 798
BaeGI GKGCMC 1 cut(s) 912
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 4 cut(s) 542, 596, 661, 676
BccI CCATC 2 cut(s) 47, 967
BcgI CGANNNNNNTGC 4 cut(s) 563, 597, 768, 802
BciT130I CCWGG 2 cut(s) 950, 1079
BclI TGATCA 4 cut(s) 163, 507, 544, 556
BcoDI GTCTC 3 cut(s) 271, 573, 960
BfaI CTAG 4 cut(s) 224, 813, 1167, 1206
BisI GCNGC 4 cut(s) 531, 585, 675, 690
BlsI GCNGC 4 cut(s) 532, 586, 676, 691
Bme1390I CCNGG 2 cut(s) 950, 1079
Bme18I GGWCC 2 cut(s) 364, 798
BmeRI GACNNNNNGTC 1 cut(s) 952
BmeT110I CYCGRG 2 cut(s) 338, 572
BmgT120I GGNCC 3 cut(s) 364, 798, 976
BmiI GGNNCC 2 cut(s) 799, 858
BmrFI CCNGG 2 cut(s) 950, 1079
BmrI ACTGGG 3 cut(s) 535, 1109, 1302
BmsI GCATC 2 cut(s) 477, 1264
BmtI GCTAGC 1 cut(s) 1170
BmuI ACTGGG 3 cut(s) 535, 1109, 1302
BpmI CTGGAG 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 185
BsaBI GATNNNNATC 2 cut(s) 162, 168
BsaJI CCNNGG 3 cut(s) 144, 601, 702
BsaWI WCCGGW 1 cut(s) 245
BsaXI ACNNNNNCTCC 4 cut(s) 106, 136, 386, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 179, 245
Bse1I ACTGG 4 cut(s) 125, 530, 1115, 1308
Bse3DI GCAATG 1 cut(s) 863
Bse8I GATNNNNATC 2 cut(s) 162, 168
BseBI CCWGG 2 cut(s) 950, 1079
BseDI CCNNGG 3 cut(s) 144, 601, 702
BseGI GGATG 3 cut(s) 928, 978, 1264
BseJI GATNNNNATC 2 cut(s) 162, 168
BseLI CCNNNNNNNGG 3 cut(s) 71, 179, 245
BseMI GCAATG 1 cut(s) 863
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 4 cut(s) 125, 530, 1115, 1308
BseSI GKGCMC 1 cut(s) 912
BseXI GCAGC 4 cut(s) 542, 596, 661, 676
BsgI GTGCAG 1 cut(s) 1126
BshFI GGCC 2 cut(s) 977, 1021
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 2 cut(s) 338, 572
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 3 cut(s) 71, 179, 245
BsmAI GTCTC 3 cut(s) 271, 573, 960
BsnI GGCC 2 cut(s) 977, 1021
BsoBI CYCGRG 2 cut(s) 338, 572
Bsp1286I GDGCHC 2 cut(s) 345, 912
Bsp143I GATC 6 cut(s) 163, 252, 507, 544, 556, 825
Bsp19I CCATGG 1 cut(s) 702
BspACI CCGC 1 cut(s) 917
BspANI GGCC 2 cut(s) 977, 1021
BspCNI CTCAG 1 cut(s) 890
BspLI GGNNCC 2 cut(s) 799, 858
BspOI GCTAGC 1 cut(s) 1170
BspPI GGATC 2 cut(s) 260, 820
BsrDI GCAATG 1 cut(s) 863
BsrI ACTGG 4 cut(s) 125, 530, 1115, 1308
BssECI CCNNGG 3 cut(s) 144, 601, 702
BssMI GATC 6 cut(s) 163, 252, 507, 544, 556, 825
BssT1I CCWWGG 3 cut(s) 144, 601, 702
Bst2UI CCWGG 2 cut(s) 950, 1079
Bst4CI ACNGT 6 cut(s) 390, 415, 449, 592, 835, 1062
BstAPI GCANNNNNTGC 1 cut(s) 1104
BstC8I GCNNGC 2 cut(s) 1145, 1168
BstDEI CTNAG 1 cut(s) 877
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 3 cut(s) 928, 978, 1264
BstKTI GATC 6 cut(s) 166, 255, 510, 547, 559, 828
BstMAI GTCTC 3 cut(s) 271, 573, 960
BstMBI GATC 6 cut(s) 163, 252, 507, 544, 556, 825
BstMWI GCNNNNNNNGC 3 cut(s) 698, 776, 1104
BstNI CCWGG 2 cut(s) 950, 1079
BstNSI RCATGY 1 cut(s) 1235
BstSCI CCNGG 2 cut(s) 948, 1077
BstSLI GKGCMC 1 cut(s) 912
BstV1I GCAGC 4 cut(s) 542, 596, 661, 676
BsuRI GGCC 2 cut(s) 977, 1021
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 3 cut(s) 928, 978, 1264
BtsI GCAGTG 2 cut(s) 614, 912
BtsIMutI CAGTG 4 cut(s) 395, 614, 912, 1315
Cac8I GCNNGC 2 cut(s) 1145, 1168
CaiI CAGNNNCTG 1 cut(s) 590
Cfr13I GGNCC 3 cut(s) 364, 798, 976
CsiI ACCWGGT 2 cut(s) 948, 1077
Csp6I GTAC 2 cut(s) 114, 816
CviAII CATG 8 cut(s) 10, 505, 703, 889, 944, 1042, 1232, 1255
CviQI GTAC 2 cut(s) 114, 816
DdeI CTNAG 1 cut(s) 877
DpnI GATC 6 cut(s) 165, 254, 509, 546, 558, 827
DpnII GATC 6 cut(s) 163, 252, 507, 544, 556, 825
DriI GACNNNNNGTC 1 cut(s) 952
Eam1105I GACNNNNNGTC 1 cut(s) 952
Eco130I CCWWGG 3 cut(s) 144, 601, 702
Eco47I GGWCC 2 cut(s) 364, 798
Eco88I CYCGRG 2 cut(s) 338, 572
EcoO109I RGGNCCY 1 cut(s) 798
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 2 cut(s) 948, 1077
EcoT14I CCWWGG 3 cut(s) 144, 601, 702
ErhI CCWWGG 3 cut(s) 144, 601, 702
FaeI CATG 8 cut(s) 13, 508, 706, 892, 947, 1045, 1235, 1258
FatI CATG 8 cut(s) 9, 504, 702, 888, 943, 1041, 1231, 1254
FauNDI CATATG 1 cut(s) 994
FbaI TGATCA 4 cut(s) 163, 507, 544, 556
Fnu4HI GCNGC 4 cut(s) 531, 585, 675, 690
FokI GGATG 3 cut(s) 935, 985, 1271
Fsp4HI GCNGC 4 cut(s) 531, 585, 675, 690
FspBI CTAG 4 cut(s) 224, 813, 1167, 1206
GluI GCNGC 4 cut(s) 531, 585, 675, 690
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 2 cut(s) 977, 1021
HapII CCGG 1 cut(s) 246
Hin1II CATG 8 cut(s) 13, 508, 706, 892, 947, 1045, 1235, 1258
HindIII AAGCTT 1 cut(s) 436
HpaII CCGG 1 cut(s) 246
HphI GGTGA 3 cut(s) 194, 617, 847
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 7 cut(s) 157, 163, 217, 257, 331, 1197, 1302
Hpy188III TCNNGA 5 cut(s) 30, 202, 338, 572, 829
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 4 cut(s) 57, 281, 958, 1211
HpyCH4III ACNGT 6 cut(s) 390, 415, 449, 592, 835, 1062
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 9 cut(s) 584, 721, 779, 905, 943, 1041, 1107, 1277, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 698, 776, 1104
HpyF3I CTNAG 1 cut(s) 877
HpySE526I ACGT 1 cut(s) 651
Hsp92II CATG 8 cut(s) 13, 508, 706, 892, 947, 1045, 1235, 1258
Ksp22I TGATCA 4 cut(s) 163, 507, 544, 556
Kzo9I GATC 6 cut(s) 163, 252, 507, 544, 556, 825
Lsp1109I GCAGC 4 cut(s) 542, 596, 661, 676
LweI GCATC 2 cut(s) 477, 1264
MabI ACCWGGT 2 cut(s) 948, 1077
MaeI CTAG 4 cut(s) 224, 813, 1167, 1206
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 5 cut(s) 477, 710, 745, 988, 1156
MalI GATC 6 cut(s) 165, 254, 509, 546, 558, 827
MboI GATC 6 cut(s) 163, 252, 507, 544, 556, 825
MboII GAAGA 1 cut(s) 161
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 2 cut(s) 345, 912
MluCI AATT 9 cut(s) 175, 333, 382, 406, 462, 790, 838, 1004, 1237
MlyI GAGTC 3 cut(s) 273, 300, 395
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 7 cut(s) 120, 349, 704, 739, 870, 913, 963
MseI TTAA 1 cut(s) 410
MslI CAYNNNNRTG 5 cut(s) 473, 765, 984, 1257, 1259
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 2 cut(s) 950, 1079
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 2 cut(s) 950, 1079
MwoI GCNNNNNNNGC 3 cut(s) 698, 776, 1104
NcoI CCATGG 1 cut(s) 702
NdeI CATATG 1 cut(s) 994
NdeII GATC 6 cut(s) 163, 252, 507, 544, 556, 825
NheI GCTAGC 1 cut(s) 1166
NlaIII CATG 8 cut(s) 13, 508, 706, 892, 947, 1045, 1235, 1258
NlaIV GGNNCC 2 cut(s) 799, 858
NmeAIII GCCGAG 1 cut(s) 1024
NmuCI GTSAC 4 cut(s) 477, 710, 745, 1156
NspI RCATGY 1 cut(s) 1235
PaeR7I CTCGAG 1 cut(s) 338
PciI ACATGT 1 cut(s) 1231
PfeI GAWTC 9 cut(s) 152, 158, 312, 610, 663, 808, 1049, 1110, 1303
PkrI GCNGC 4 cut(s) 532, 586, 676, 691
PleI GAGTC 3 cut(s) 272, 299, 395
PpsI GAGTC 3 cut(s) 272, 299, 395
PpuMI RGGWCCY 1 cut(s) 798
PscI ACATGT 1 cut(s) 1231
Psp5II RGGWCCY 1 cut(s) 798
Psp6I CCWGG 2 cut(s) 948, 1077
PspGI CCWGG 2 cut(s) 948, 1077
PspN4I GGNNCC 2 cut(s) 799, 858
PspPI GGNCC 3 cut(s) 364, 798, 976
PspPPI RGGWCCY 1 cut(s) 798
PstNI CAGNNNCTG 1 cut(s) 590
RsaI GTAC 2 cut(s) 115, 817
RsaNI GTAC 2 cut(s) 114, 816
RseI CAYNNNNRTG 5 cut(s) 473, 765, 984, 1257, 1259
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 4 cut(s) 531, 585, 675, 690
Sau3AI GATC 6 cut(s) 163, 252, 507, 544, 556, 825
Sau96I GGNCC 3 cut(s) 364, 798, 976
SchI GAGTC 3 cut(s) 273, 300, 395
ScrFI CCNGG 2 cut(s) 950, 1079
SduI GDGCHC 2 cut(s) 345, 912
SexAI ACCWGGT 2 cut(s) 948, 1077
SfaNI GCATC 2 cut(s) 477, 1264
Sfr274I CTCGAG 1 cut(s) 338
SinI GGWCC 2 cut(s) 364, 798
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 5 cut(s) 473, 765, 984, 1257, 1259
SmlI CTYRAG 2 cut(s) 200, 338
SmoI CTYRAG 2 cut(s) 200, 338
Sse9I AATT 9 cut(s) 175, 333, 382, 406, 462, 790, 838, 1004, 1237
SsiI CCGC 1 cut(s) 917
SspMI CTAG 4 cut(s) 224, 813, 1167, 1206
StyD4I CCNGG 2 cut(s) 948, 1077
StyI CCWWGG 3 cut(s) 144, 601, 702
TaaI ACNGT 6 cut(s) 390, 415, 449, 592, 835, 1062
TaiI ACGT 1 cut(s) 654
TaqI TCGA 2 cut(s) 339, 788
TasI AATT 9 cut(s) 175, 333, 382, 406, 462, 790, 838, 1004, 1237
TfiI GAWTC 9 cut(s) 152, 158, 312, 610, 663, 808, 1049, 1110, 1303
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TscAI CASTG 4 cut(s) 395, 621, 912, 1315
TseFI GTSAC 4 cut(s) 477, 710, 745, 1156
TseI GCWGC 4 cut(s) 530, 584, 674, 689
Tsp45I GTSAC 4 cut(s) 477, 710, 745, 1156
TspDTI ATGAA 7 cut(s) 300, 505, 749, 977, 1058, 1137, 1193
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 4 cut(s) 395, 621, 912, 1315
VpaK11BI GGWCC 2 cut(s) 364, 798
XapI RAATTY 5 cut(s) 333, 790, 838, 1004, 1237
XceI RCATGY 1 cut(s) 1235
XhoI CTCGAG 1 cut(s) 338
XspI CTAG 4 cut(s) 224, 813, 1167, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.