Rw4G026600

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017288
Physical Location & Seq
Reverse (-)
52823053 .. 52824447
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026600.1

Sequence Viewer

Length: 1326 bp
ATGGAAGTTAAAATAATATCCAGAGAAACCATCAAACCTTCCACTCCAACCCTACCACATCACGGAACATACCACCTTTCTTTCCTTGAACAGATAAATCCTCGTACCTATACACCAGTTGTTTACTTCTACCCCAAGGAAGAATCTGATTCTGATCATAATCCTAATTTTGGTGATAAATCCAATCAGCTCAAGAAATCTCTATCAGAAACACTAGCCAAATACTACCCATTTTCCGGTAGGATCAGAGATCGAGTCTCTATTGATTGTAATGATGAAGGAGTCATATTTGTAGAAGCAAGAATCAAAGTCAAGCTCTCTGAAATTCTGGAGCACCCGAAAGATGAGGTGCTGGACCTGTTATTCACAGACAATTTACAGTGGAACGATGACTCCAATTTAACTGTCATCTTGGCTGTTCAAGTAAGCTTCTTCGACTGTGGTGGAATGGCAATTGGCATCTGTATGTCACACAAAGTAGCTGACACTTGCACCATGATCAACTTCATCAACGACTGGGCAGCCATAACCTGTGATCACAAAGCTGATCAATATGTGTCTCCCGAGTTTATTGCAGCAACTGTATTCCCCCAAGGTGAGATTCCACTGCTACCGCAATCTGTGATTGAGAAAGGGGATTACGTTTCCAAGAGATTTGTGTTTGCTGCCCCGAAAGTTGCTGCCCTCAAAGCCATGGTGTGTGACAAAGTGCAAAACCCCTCAAAAGTTGAAGTTGTGACTGCTTTTATTCATAGATGTGCCATATCTGCATTGAAATCGAATTCTGGGTCCTTGTTGAATCCTAGTACCCTTTTGATCCAGACGGTGAATTTGCGTGGTAGAATGGTTCCTCCATTGCCGAAAAGCTCAGTAGGTAGCATGGTTTGGTTCTATGCAGTGCCCACAGCGGAGGATGAGAGTGTGATAGAGTTGCATGACCTGGTGTCTCAAATGAAGGAGAGGATGGCTCATTTCTGTGTTACATATGCCGAGAAATTTGGAAAAGAATGGCCTTTGATATTTATGGAGTGCATGAAAGATTCAAGGAAACTGTTTCAAAAACAGAACCTGGTTGTGTATAGATGTAGCAGTTGGTGCAGATTCCCAGTTTACGAAGCAGACTTTGGTTGGGGCAAGCCAATTTGGGTCACTATTGCTAGCTGTGTGATGAAAAATAGTATCTTTCTGATTGATACTAGGAATGGAGAAGGGATTGAAGCACATGTGAATTTGGAAAAGCAACACATGGATGTGTTTGCGCGTGATGCAGAGCTTCTTGCATTTGCTTCTCTGAATCCCAGTGCTAATATAGACTACAACATGTGA

Protein Analysis

441

Amino Acids

49.85

Weight (kDa)

5.88

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 425 3.5e-86 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017288)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24550
malus_domestica MD16G1110600.v1.1 MD16G1110700.v1.1
pyrus_communis pycom16g09390
rosa_chinensis RchiOBHm_Chr4g0431311 RchiOBHm_Chr4g0431461
rosa_laevigata RLG00000006912
rosa_multiflora Rmu_sc0007885.1_g000012
rosa_roxburghii Rroxscaffold_5G00372910
rosa_rugosa Rorug04G0250400
rosa_samantha Rh4AG306500 Rh4DG309700
rosa_wichuraiana Rw4G026600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1261
AciI CCGC 2 cut(s) 614, 908
AclWI GGATC 2 cut(s) 251, 811
AcsI RAATTY 5 cut(s) 324, 781, 829, 995, 1228
AfaI GTAC 2 cut(s) 106, 808
AfiI CCNNNNNNNGG 3 cut(s) 62, 170, 236
AflIII ACRYGT 2 cut(s) 1222, 1320
AgsI TTSAA 8 cut(s) 89, 422, 731, 775, 799, 1044, 1058, 1217
AhdI GACNNNNNGTC 1 cut(s) 943
AjnI CCWGG 2 cut(s) 939, 1068
AjuI GAANNNNNNNTTGG 4 cut(s) 585, 617, 1107, 1139
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
AluBI AGCT 8 cut(s) 190, 316, 429, 482, 545, 867, 1161, 1273
AluI AGCT 8 cut(s) 190, 316, 429, 482, 545, 867, 1161, 1273
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 3 cut(s) 262, 564, 951
AlwI GGATC 2 cut(s) 251, 811
AlwNI CAGNNNCTG 2 cut(s) 581, 1069
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 1 cut(s) 1010
ApeKI GCWGC 4 cut(s) 521, 575, 665, 680
ApoI RAATTY 5 cut(s) 324, 781, 829, 995, 1228
ArsI GACNNNNNNTTYG 4 cut(s) 773, 805, 814, 846
AspLEI GCGC 1 cut(s) 1261
AspS9I GGNCC 2 cut(s) 355, 789
AsuHPI GGTGA 3 cut(s) 185, 608, 838
AsuNHI GCTAGC 1 cut(s) 1157
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 2 cut(s) 355, 789
BaeGI GKGCMC 1 cut(s) 903
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 4 cut(s) 533, 587, 652, 667
BccI CCATC 2 cut(s) 38, 958
BcgI CGANNNNNNTGC 4 cut(s) 554, 588, 759, 793
BciT130I CCWGG 2 cut(s) 941, 1070
BclI TGATCA 4 cut(s) 154, 498, 535, 547
BcoDI GTCTC 3 cut(s) 262, 564, 951
BfaI CTAG 4 cut(s) 215, 804, 1158, 1197
BisI GCNGC 4 cut(s) 522, 576, 666, 681
BlsI GCNGC 4 cut(s) 523, 577, 667, 682
Bme1390I CCNGG 2 cut(s) 941, 1070
Bme18I GGWCC 2 cut(s) 355, 789
BmeRI GACNNNNNGTC 1 cut(s) 943
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 355, 789
BmiI GGNNCC 2 cut(s) 790, 849
BmrFI CCNGG 2 cut(s) 941, 1070
BmrI ACTGGG 3 cut(s) 526, 1100, 1293
BmsI GCATC 2 cut(s) 468, 1255
BmtI GCTAGC 1 cut(s) 1161
BmuI ACTGGG 3 cut(s) 526, 1100, 1293
BplI GAGNNNNNCTC 2 cut(s) 952, 984
BpmI CTGGAG 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 176
BsaBI GATNNNNATC 2 cut(s) 153, 159
BsaJI CCNNGG 3 cut(s) 135, 592, 693
BsaWI WCCGGW 1 cut(s) 236
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 170, 236
Bse1I ACTGG 4 cut(s) 116, 521, 1106, 1299
Bse3DI GCAATG 1 cut(s) 854
Bse8I GATNNNNATC 2 cut(s) 153, 159
BseBI CCWGG 2 cut(s) 941, 1070
BseDI CCNNGG 3 cut(s) 135, 592, 693
BseGI GGATG 3 cut(s) 919, 969, 1255
BseJI GATNNNNATC 2 cut(s) 153, 159
BseLI CCNNNNNNNGG 3 cut(s) 62, 170, 236
BseMI GCAATG 1 cut(s) 854
BseMII CTCAG 1 cut(s) 882
BseNI ACTGG 4 cut(s) 116, 521, 1106, 1299
BseSI GKGCMC 1 cut(s) 903
BseXI GCAGC 4 cut(s) 533, 587, 652, 667
BsgI GTGCAG 1 cut(s) 1117
Bsh1236I CGCG 1 cut(s) 1261
BshFI GGCC 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 3 cut(s) 62, 170, 236
BsmAI GTCTC 3 cut(s) 262, 564, 951
BsnI GGCC 1 cut(s) 1012
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 336, 903
Bsp143I GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 2 cut(s) 614, 908
BspANI GGCC 1 cut(s) 1012
BspCNI CTCAG 1 cut(s) 881
BspFNI CGCG 1 cut(s) 1261
BspLI GGNNCC 2 cut(s) 790, 849
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 2 cut(s) 251, 811
BsrDI GCAATG 1 cut(s) 854
BsrI ACTGG 4 cut(s) 116, 521, 1106, 1299
BssECI CCNNGG 3 cut(s) 135, 592, 693
BssMI GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
BssT1I CCWWGG 3 cut(s) 135, 592, 693
Bst2UI CCWGG 2 cut(s) 941, 1070
Bst4CI ACNGT 6 cut(s) 381, 406, 440, 583, 826, 1053
BstAPI GCANNNNNTGC 1 cut(s) 1095
BstC8I GCNNGC 2 cut(s) 1136, 1159
BstDEI CTNAG 1 cut(s) 868
BstDSI CCRYGG 1 cut(s) 693
BstF5I GGATG 3 cut(s) 919, 969, 1255
BstFNI CGCG 1 cut(s) 1261
BstHHI GCGC 1 cut(s) 1261
BstKTI GATC 7 cut(s) 157, 246, 253, 501, 538, 550, 819
BstMAI GTCTC 3 cut(s) 262, 564, 951
BstMBI GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
BstMWI GCNNNNNNNGC 4 cut(s) 689, 767, 1095, 1265
BstNI CCWGG 2 cut(s) 941, 1070
BstNSI RCATGY 2 cut(s) 1226, 1324
BstSCI CCNGG 2 cut(s) 939, 1068
BstSLI GKGCMC 1 cut(s) 903
BstUI CGCG 1 cut(s) 1261
BstV1I GCAGC 4 cut(s) 533, 587, 652, 667
BsuRI GGCC 1 cut(s) 1012
BtgI CCRYGG 1 cut(s) 693
BtsCI GGATG 3 cut(s) 919, 969, 1255
BtsI GCAGTG 2 cut(s) 605, 903
BtsIMutI CAGTG 4 cut(s) 386, 605, 903, 1306
Cac8I GCNNGC 2 cut(s) 1136, 1159
CaiI CAGNNNCTG 2 cut(s) 581, 1069
CfoI GCGC 1 cut(s) 1261
Cfr13I GGNCC 2 cut(s) 355, 789
CsiI ACCWGGT 2 cut(s) 939, 1068
Csp6I GTAC 2 cut(s) 105, 807
CviAII CATG 8 cut(s) 496, 694, 880, 935, 1033, 1223, 1246, 1321
CviQI GTAC 2 cut(s) 105, 807
DdeI CTNAG 1 cut(s) 868
DpnI GATC 7 cut(s) 156, 245, 252, 500, 537, 549, 818
DpnII GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
DriI GACNNNNNGTC 1 cut(s) 943
Eam1105I GACNNNNNGTC 1 cut(s) 943
Eco130I CCWWGG 3 cut(s) 135, 592, 693
Eco47I GGWCC 2 cut(s) 355, 789
Eco88I CYCGRG 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 789
EcoRI GAATTC 1 cut(s) 781
EcoRII CCWGG 2 cut(s) 939, 1068
EcoT14I CCWWGG 3 cut(s) 135, 592, 693
ErhI CCWWGG 3 cut(s) 135, 592, 693
FaeI CATG 8 cut(s) 499, 697, 883, 938, 1036, 1226, 1249, 1324
FatI CATG 8 cut(s) 495, 693, 879, 934, 1032, 1222, 1245, 1320
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 4 cut(s) 154, 498, 535, 547
Fnu4HI GCNGC 4 cut(s) 522, 576, 666, 681
FokI GGATG 3 cut(s) 926, 976, 1262
Fsp4HI GCNGC 4 cut(s) 522, 576, 666, 681
FspBI CTAG 4 cut(s) 215, 804, 1158, 1197
GlaI GCGC 1 cut(s) 1260
GluI GCNGC 4 cut(s) 522, 576, 666, 681
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 1 cut(s) 1012
HapII CCGG 1 cut(s) 237
HhaI GCGC 1 cut(s) 1261
Hin1II CATG 8 cut(s) 499, 697, 883, 938, 1036, 1226, 1249, 1324
Hin6I GCGC 1 cut(s) 1259
HinP1I GCGC 1 cut(s) 1259
HindIII AAGCTT 1 cut(s) 427
HpaII CCGG 1 cut(s) 237
HphI GGTGA 3 cut(s) 185, 608, 838
Hpy166II GTNNAC 2 cut(s) 124, 1111
Hpy188I TCNGA 7 cut(s) 148, 154, 208, 248, 322, 1188, 1293
Hpy188III TCNNGA 5 cut(s) 21, 193, 329, 563, 820
Hpy8I GTNNAC 2 cut(s) 124, 1111
HpyAV CCTTC 4 cut(s) 48, 272, 949, 1202
HpyCH4III ACNGT 6 cut(s) 381, 406, 440, 583, 826, 1053
HpyCH4IV ACGT 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 4 cut(s) 689, 767, 1095, 1265
HpyF3I CTNAG 1 cut(s) 868
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 8 cut(s) 499, 697, 883, 938, 1036, 1226, 1249, 1324
HspAI GCGC 1 cut(s) 1259
Ksp22I TGATCA 4 cut(s) 154, 498, 535, 547
Kzo9I GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
LmnI GCTCC 1 cut(s) 331
Lsp1109I GCAGC 4 cut(s) 533, 587, 652, 667
LweI GCATC 2 cut(s) 468, 1255
MabI ACCWGGT 2 cut(s) 939, 1068
MaeI CTAG 4 cut(s) 215, 804, 1158, 1197
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 5 cut(s) 468, 701, 736, 979, 1147
MalI GATC 7 cut(s) 156, 245, 252, 500, 537, 549, 818
MboI GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
MboII GAAGA 2 cut(s) 152, 424
MfeI CAATTG 1 cut(s) 453
MhlI GDGCHC 2 cut(s) 336, 903
MlyI GAGTC 3 cut(s) 264, 291, 386
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 7 cut(s) 111, 340, 695, 730, 861, 904, 954
MseI TTAA 2 cut(s) 9, 401
MslI CAYNNNNRTG 5 cut(s) 464, 756, 975, 1248, 1250
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 2 cut(s) 941, 1070
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 2 cut(s) 941, 1070
MvnI CGCG 1 cut(s) 1261
MwoI GCNNNNNNNGC 4 cut(s) 689, 767, 1095, 1265
NcoI CCATGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 985
NdeII GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
NheI GCTAGC 1 cut(s) 1157
NlaIII CATG 8 cut(s) 499, 697, 883, 938, 1036, 1226, 1249, 1324
NlaIV GGNNCC 2 cut(s) 790, 849
NmeAIII GCCGAG 1 cut(s) 1015
NmuCI GTSAC 4 cut(s) 468, 701, 736, 1147
NspI RCATGY 2 cut(s) 1226, 1324
PciI ACATGT 2 cut(s) 1222, 1320
PfeI GAWTC 8 cut(s) 143, 149, 303, 601, 799, 1040, 1101, 1294
PkrI GCNGC 4 cut(s) 523, 577, 667, 682
PleI GAGTC 3 cut(s) 263, 290, 386
PpsI GAGTC 3 cut(s) 263, 290, 386
PpuMI RGGWCCY 1 cut(s) 789
PscI ACATGT 2 cut(s) 1222, 1320
Psp5II RGGWCCY 1 cut(s) 789
Psp6I CCWGG 2 cut(s) 939, 1068
PspGI CCWGG 2 cut(s) 939, 1068
PspN4I GGNNCC 2 cut(s) 790, 849
PspPI GGNCC 2 cut(s) 355, 789
PspPPI RGGWCCY 1 cut(s) 789
PstNI CAGNNNCTG 2 cut(s) 581, 1069
RsaI GTAC 2 cut(s) 106, 808
RsaNI GTAC 2 cut(s) 105, 807
RseI CAYNNNNRTG 5 cut(s) 464, 756, 975, 1248, 1250
SaqAI TTAA 2 cut(s) 9, 401
SatI GCNGC 4 cut(s) 522, 576, 666, 681
Sau3AI GATC 7 cut(s) 154, 243, 250, 498, 535, 547, 816
Sau96I GGNCC 2 cut(s) 355, 789
SchI GAGTC 3 cut(s) 264, 291, 386
ScrFI CCNGG 2 cut(s) 941, 1070
SduI GDGCHC 2 cut(s) 336, 903
SexAI ACCWGGT 2 cut(s) 939, 1068
SfaNI GCATC 2 cut(s) 468, 1255
SinI GGWCC 2 cut(s) 355, 789
SmiMI CAYNNNNRTG 5 cut(s) 464, 756, 975, 1248, 1250
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SsiI CCGC 2 cut(s) 614, 908
SspMI CTAG 4 cut(s) 215, 804, 1158, 1197
StyD4I CCNGG 2 cut(s) 939, 1068
StyI CCWWGG 3 cut(s) 135, 592, 693
TaaI ACNGT 6 cut(s) 381, 406, 440, 583, 826, 1053
TaiI ACGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 253, 435, 779
TfiI GAWTC 8 cut(s) 143, 149, 303, 601, 799, 1040, 1101, 1294
Tru1I TTAA 2 cut(s) 9, 401
Tru9I TTAA 2 cut(s) 9, 401
TscAI CASTG 4 cut(s) 386, 612, 903, 1306
TseFI GTSAC 4 cut(s) 468, 701, 736, 1147
TseI GCWGC 4 cut(s) 521, 575, 665, 680
Tsp45I GTSAC 4 cut(s) 468, 701, 736, 1147
TspDTI ATGAA 6 cut(s) 291, 496, 740, 968, 1049, 1184
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 4 cut(s) 386, 612, 903, 1306
VpaK11BI GGWCC 2 cut(s) 355, 789
XapI RAATTY 5 cut(s) 324, 781, 829, 995, 1228
XceI RCATGY 2 cut(s) 1226, 1324
XspI CTAG 4 cut(s) 215, 804, 1158, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.