MD16G1110700.v1.1

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017288
Physical Location & Seq
Forward (+)
7784318 .. 7785631
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1110700.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGACCATGGAAGTTGAAATCATATCCAGAGAAATCATCAAACCTTCCACTCCAACCCCACCACATCTTCGAACACACAACCTTTCATTCCTCGAACAGAATACTCCTCGTACTTATCTGCCGGTGGTTTACTTCTATCACAAGGAGAGCTCTGCTTCTACTAAAGGCGATCACAAATCCAATCAGCTCAAGAAATCTTTATCAGAAATACTATCCAAGTACTACCCGTTCGCCGGCAGGATCAGAGACCGAGCCTCCGTTGACTGCAACGATGAAGGAGTTGCGTTTGTCGAAGCAAGAGTCAGAAACATCAAGCTATCTGAAATTCTTGAACACCCTGAAGATACATGGCTGGACTTGTTATTCACAGACAATTTACAGTGGAACGATGATTCGAGTCTCAGCGTGATCTTTGCTGTTCAAGTAAGCTTCTTTGACTGTGGTGGCATGGCAATTGGCATCTGTATGTCACACAAAGTAGCTGACACATCCACCGTGGCCAACTTCATCAACGACTGGGCTGCTCGAAACAAGTGTGATCAATATGTACCGTCTCCTTTGTTCATCGGAGCAGATACATTTCCCCAAGGTGACATGCCATTGTTCCCGGAAACTGTGCTCGAAAAAGGCGACTGCGTCACCAAGAGATTTGTGTTTGATGCCCCGAAGATTGCTTCCCTCAAAGCAATAGTCGCGGACAAAGTGCAAAACCCCACAAGGGTTGAAGTTGTGACAGCTTTGATTTACAGCTGTGCAATTTCTGCGTTAAGATCAACTTCACAGTCGTCCTTAAACCCGACAGTTTTCATCCATGCAGTGAATCTCCGCACTAGGGTGATCCCTCCGTTGCCGAAAAATTCTGTAGGCAGCATGATTTGGGCTTTTACATTGTCCACAGCTGAGGACGGTGTGATCGAGTTGCATGACCTGGTGACTCAAATGAAGCAGAGCATGACCCGATTTTGTGATTCTTATGCTCAAAAGTTTCGCGGGGAAGATCAATGGCCGGCAATCTTTAAAGAGTGTACGCAAGCATTAAGGAAAGAATTTTCGATAGCGAATTTGGTGACGTACAGATGTAGCAGCTGGTGCAGGTTCCCAGTGTATGAAGCTGACTTTGGCTGGGGGAAGCCAATTTGGGTGACTATTGCGAGCTGTTCCCTGAAGAATGCTATTTTTCTGATTGATACAAGGAATGGAGAAGGGATTGAAGCATATGTGAATTTGGAAAAGCAACAATTGGATGTGTTCGAGCGCGACGCGCAGCTTCTTGCATTTGCTTCTCTGAATCCGAGTGCTTTAGACTCCATCTGA

Protein Analysis

438

Amino Acids

49.13

Weight (kDa)

5.71

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 423 3e-83 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017288)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24550
malus_domestica MD16G1110600.v1.1 MD16G1110700.v1.1
pyrus_communis pycom16g09390
rosa_chinensis RchiOBHm_Chr4g0431311 RchiOBHm_Chr4g0431461
rosa_laevigata RLG00000006912
rosa_multiflora Rmu_sc0007885.1_g000012
rosa_roxburghii Rroxscaffold_5G00372910
rosa_rugosa Rorug04G0250400
rosa_samantha Rh4AG306500 Rh4DG309700
rosa_wichuraiana Rw4G026600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1083
AccII CGCG 4 cut(s) 695, 990, 1257, 1262
AciI CCGC 3 cut(s) 695, 826, 990
AclWI GGATC 2 cut(s) 248, 832
AcoI YGGCCR 2 cut(s) 498, 1004
AcsI RAATTY 5 cut(s) 324, 856, 1046, 1060, 1222
AcuI CTGAAG 2 cut(s) 360, 1184
AfaI GTAC 5 cut(s) 112, 221, 549, 1027, 1073
AfiI CCNNNNNNNGG 3 cut(s) 233, 718, 832
AgsI TTSAA 5 cut(s) 17, 332, 422, 725, 1211
AjnI CCWGG 1 cut(s) 927
AjuI GAANNNNNNNTTGG 2 cut(s) 1101, 1133
AleI CACNNNNGTG 1 cut(s) 833
Alw21I GWGCWC 2 cut(s) 152, 621
Alw26I GTCTC 3 cut(s) 240, 404, 558
AlwI GGATC 2 cut(s) 248, 832
AoxI GGCC 2 cut(s) 498, 1004
ApeKI GCWGC 4 cut(s) 521, 867, 1083, 1264
ApoI RAATTY 5 cut(s) 324, 856, 1046, 1060, 1222
ArsI GACNNNNNNTTYG 4 cut(s) 285, 317, 675, 707
AspLEI GCGC 2 cut(s) 1257, 1264
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 6 cut(s) 602, 631, 847, 943, 1078, 1153
AsuII TTCGAA 1 cut(s) 70
BalI TGGCCA 1 cut(s) 500
BanII GRGCYC 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 152, 621
BbvCI CCTCAGC 1 cut(s) 900
BbvI GCAGC 4 cut(s) 508, 879, 1095, 1276
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 1 cut(s) 929
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 3 cut(s) 240, 404, 558
BfaI CTAG 1 cut(s) 831
BfmI CTRYAG 1 cut(s) 861
BfuAI ACCTGC 1 cut(s) 1083
BisI GCNGC 4 cut(s) 522, 868, 1084, 1265
BlsI GCNGC 4 cut(s) 523, 869, 1085, 1266
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 2 cut(s) 608, 929
BmiI GGNNCC 1 cut(s) 1097
BmrFI CCNGG 2 cut(s) 608, 929
BmrI ACTGGG 2 cut(s) 526, 1094
BmsI GCATC 2 cut(s) 468, 649
BmuI ACTGGG 2 cut(s) 526, 1094
Bpu10I CCTNAGC 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 6, 495, 586
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 718, 832
Bse118I RCCGGY 3 cut(s) 121, 233, 1006
Bse1I ACTGG 2 cut(s) 521, 1100
BseBI CCWGG 1 cut(s) 929
BseDI CCNNGG 3 cut(s) 6, 495, 586
BseGI GGATG 3 cut(s) 488, 807, 1249
BseLI CCNNNNNNNGG 3 cut(s) 233, 718, 832
BseMII CTCAG 2 cut(s) 415, 891
BseNI ACTGG 2 cut(s) 521, 1100
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 4 cut(s) 508, 879, 1095, 1276
BseYI CCCAGC 1 cut(s) 1122
BsgI GTGCAG 1 cut(s) 1111
Bsh1236I CGCG 4 cut(s) 695, 990, 1257, 1262
BshFI GGCC 2 cut(s) 500, 1006
BsiHKAI GWGCWC 2 cut(s) 152, 621
BsiSI CCGG 4 cut(s) 122, 234, 608, 1007
BslI CCNNNNNNNGG 3 cut(s) 233, 718, 832
BsmAI GTCTC 3 cut(s) 240, 404, 558
BsmBI CGTCTC 1 cut(s) 558
BsmI GAATGC 1 cut(s) 1174
BsnI GGCC 2 cut(s) 500, 1006
Bso31I GGTCTC 1 cut(s) 240
Bsp119I TTCGAA 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 152, 621
Bsp143I GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 695, 826, 990
BspANI GGCC 2 cut(s) 500, 1006
BspCNI CTCAG 2 cut(s) 414, 892
BspFNI CGCG 4 cut(s) 695, 990, 1257, 1262
BspLI GGNNCC 1 cut(s) 1097
BspMI ACCTGC 1 cut(s) 1083
BspPI GGATC 2 cut(s) 248, 832
BspT104I TTCGAA 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 240
BsrFI RCCGGY 3 cut(s) 121, 233, 1006
BsrI ACTGG 2 cut(s) 521, 1100
BssAI RCCGGY 3 cut(s) 121, 233, 1006
BssECI CCNNGG 3 cut(s) 6, 495, 586
BssMI GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
BssT1I CCWWGG 2 cut(s) 6, 586
Bst2UI CCWGG 1 cut(s) 929
Bst4CI ACNGT 8 cut(s) 381, 440, 496, 552, 616, 783, 802, 908
BstAPI GCANNNNNTGC 2 cut(s) 761, 1089
BstBI TTCGAA 1 cut(s) 70
BstC8I GCNNGC 4 cut(s) 235, 1008, 1032, 1153
BstDEI CTNAG 2 cut(s) 401, 900
BstDSI CCRYGG 2 cut(s) 6, 495
BstF5I GGATG 3 cut(s) 488, 807, 1249
BstFNI CGCG 4 cut(s) 695, 990, 1257, 1262
BstHHI GCGC 2 cut(s) 1257, 1264
BstKTI GATC 8 cut(s) 172, 243, 411, 541, 773, 840, 915, 1000
BstMAI GTCTC 3 cut(s) 240, 404, 558
BstMBI GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
BstMWI GCNNNNNNNGC 4 cut(s) 692, 761, 1089, 1261
BstNI CCWGG 1 cut(s) 929
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 2 cut(s) 606, 927
BstSFI CTRYAG 1 cut(s) 861
BstUI CGCG 4 cut(s) 695, 990, 1257, 1262
BstV1I GCAGC 4 cut(s) 508, 879, 1095, 1276
BsuRI GGCC 2 cut(s) 500, 1006
BtgI CCRYGG 2 cut(s) 6, 495
BtsCI GGATG 3 cut(s) 488, 807, 1249
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 3 cut(s) 386, 822, 1107
BveI ACCTGC 1 cut(s) 1083
Cac8I GCNNGC 4 cut(s) 235, 1008, 1032, 1153
CfoI GCGC 2 cut(s) 1257, 1264
Cfr10I RCCGGY 3 cut(s) 121, 233, 1006
CseI GACGC 2 cut(s) 625, 1268
CsiI ACCWGGT 1 cut(s) 927
Csp6I GTAC 5 cut(s) 111, 220, 548, 1026, 1072
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviAII CATG 8 cut(s) 7, 348, 448, 595, 812, 871, 923, 952
CviQI GTAC 5 cut(s) 111, 220, 548, 1026, 1072
DdeI CTNAG 2 cut(s) 401, 900
DpnI GATC 8 cut(s) 171, 242, 410, 540, 772, 839, 914, 999
DpnII GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
DraI TTTAAA 1 cut(s) 1018
EaeI YGGCCR 2 cut(s) 498, 1004
Ecl136II GAGCTC 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 6, 586
Eco24I GRGCYC 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 360, 1184
EcoICRI GAGCTC 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 927
EcoT14I CCWWGG 2 cut(s) 6, 586
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 2 cut(s) 6, 586
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 8 cut(s) 10, 351, 451, 598, 815, 874, 926, 955
FalI AAGNNNNNCTT 2 cut(s) 760, 792
FatI CATG 8 cut(s) 6, 347, 447, 594, 811, 870, 922, 951
FauI CCCGC 1 cut(s) 983
FauNDI CATATG 1 cut(s) 1216
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 4 cut(s) 522, 868, 1084, 1265
FokI GGATG 3 cut(s) 475, 794, 1256
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 4 cut(s) 522, 868, 1084, 1265
FspBI CTAG 1 cut(s) 831
GlaI GCGC 2 cut(s) 1256, 1263
GluI GCNGC 4 cut(s) 522, 868, 1084, 1265
GsaI CCCAGC 1 cut(s) 1126
HaeIII GGCC 2 cut(s) 500, 1006
HapII CCGG 4 cut(s) 122, 234, 608, 1007
HgaI GACGC 2 cut(s) 625, 1268
HhaI GCGC 2 cut(s) 1257, 1264
Hin1II CATG 8 cut(s) 10, 351, 451, 598, 815, 874, 926, 955
Hin6I GCGC 2 cut(s) 1255, 1262
HinP1I GCGC 2 cut(s) 1255, 1262
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 427
HinfI GANTC 8 cut(s) 300, 392, 397, 820, 934, 968, 1288, 1304
HpaII CCGG 4 cut(s) 122, 234, 608, 1007
HphI GGTGA 6 cut(s) 602, 631, 847, 943, 1078, 1153
Hpy166II GTNNAC 4 cut(s) 130, 262, 894, 1026
Hpy188I TCNGA 9 cut(s) 205, 245, 305, 322, 569, 1182, 1287, 1293, 1313
Hpy188III TCNNGA 3 cut(s) 27, 190, 329
Hpy8I GTNNAC 4 cut(s) 130, 262, 894, 1026
Hpy99I CGWCG 1 cut(s) 1262
HpyAV CCTTC 3 cut(s) 54, 269, 1196
HpyCH4III ACNGT 8 cut(s) 381, 440, 496, 552, 616, 783, 802, 908
HpyCH4IV ACGT 1 cut(s) 1070
HpyCH4V TGCA 7 cut(s) 267, 706, 755, 815, 922, 1092, 1274
HpyF10VI GCNNNNNNNGC 4 cut(s) 692, 761, 1089, 1261
HpyF3I CTNAG 2 cut(s) 401, 900
HpySE526I ACGT 1 cut(s) 1070
Hsp92II CATG 8 cut(s) 10, 351, 451, 598, 815, 874, 926, 955
HspAI GCGC 2 cut(s) 1255, 1262
KroI GCCGGC 2 cut(s) 233, 1006
KroNI GCCGGC 2 cut(s) 235, 1008
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
LmnI GCTCC 1 cut(s) 569
Lsp1109I GCAGC 4 cut(s) 508, 879, 1095, 1276
LweI GCATC 2 cut(s) 468, 649
MabI ACCWGGT 1 cut(s) 927
MaeI CTAG 1 cut(s) 831
MaeII ACGT 1 cut(s) 1070
MaeIII GTNAC 7 cut(s) 468, 590, 637, 730, 931, 1066, 1141
MalI GATC 8 cut(s) 171, 242, 410, 540, 772, 839, 914, 999
MboI GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
MboII GAAGA 5 cut(s) 59, 353, 679, 1007, 1177
MfeI CAATTG 2 cut(s) 453, 1238
MhlI GDGCHC 2 cut(s) 152, 621
MlsI TGGCCA 1 cut(s) 500
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 4 cut(s) 309, 406, 928, 1298
MmeI TCCRAC 1 cut(s) 77
MnlI CCTC 6 cut(s) 101, 117, 265, 689, 852, 895
Mox20I TGGCCA 1 cut(s) 500
MroNI GCCGGC 2 cut(s) 233, 1006
MscI TGGCCA 1 cut(s) 500
MseI TTAA 4 cut(s) 767, 791, 1017, 1037
MslI CAYNNNNRTG 2 cut(s) 464, 833
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 3 cut(s) 750, 899, 1086
MspI CCGG 4 cut(s) 122, 234, 608, 1007
MspR9I CCNGG 2 cut(s) 608, 929
MunI CAATTG 2 cut(s) 453, 1238
Mva1269I GAATGC 1 cut(s) 1174
MvaI CCWGG 1 cut(s) 929
MvnI CGCG 4 cut(s) 695, 990, 1257, 1262
MwoI GCNNNNNNNGC 4 cut(s) 692, 761, 1089, 1261
NaeI GCCGGC 2 cut(s) 235, 1008
NciI CCSGG 1 cut(s) 608
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 1216
NdeII GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
NgoMIV GCCGGC 2 cut(s) 233, 1006
NlaIII CATG 8 cut(s) 10, 351, 451, 598, 815, 874, 926, 955
NlaIV GGNNCC 1 cut(s) 1097
NmuCI GTSAC 7 cut(s) 468, 590, 637, 730, 931, 1066, 1141
NspI RCATGY 1 cut(s) 598
NspV TTCGAA 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 833
PcsI WCGNNNNNNNCGW 2 cut(s) 627, 912
PctI GAATGC 1 cut(s) 1174
PdiI GCCGGC 2 cut(s) 235, 1008
PfeI GAWTC 4 cut(s) 392, 820, 968, 1288
PflFI GACNNNGTC 1 cut(s) 635
PfoI TCCNGGA 1 cut(s) 606
PkrI GCNGC 4 cut(s) 523, 869, 1085, 1266
PleI GAGTC 4 cut(s) 308, 405, 928, 1298
PpsI GAGTC 4 cut(s) 308, 405, 928, 1298
Psp124BI GAGCTC 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 927
PspFI CCCAGC 1 cut(s) 1122
PspGI CCWGG 1 cut(s) 927
PspN4I GGNNCC 1 cut(s) 1097
PsrI GAACNNNNNNTAC 2 cut(s) 212, 244
PsyI GACNNNGTC 1 cut(s) 635
PvuII CAGCTG 3 cut(s) 750, 899, 1086
RsaI GTAC 5 cut(s) 112, 221, 549, 1027, 1073
RsaNI GTAC 5 cut(s) 111, 220, 548, 1026, 1072
RseI CAYNNNNRTG 2 cut(s) 464, 833
SacI GAGCTC 1 cut(s) 152
SaqAI TTAA 4 cut(s) 767, 791, 1017, 1037
SatI GCNGC 4 cut(s) 522, 868, 1084, 1265
Sau3AI GATC 8 cut(s) 169, 240, 408, 538, 770, 837, 912, 997
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 4 cut(s) 309, 406, 928, 1298
ScrFI CCNGG 2 cut(s) 608, 929
SduI GDGCHC 2 cut(s) 152, 621
SexAI ACCWGGT 1 cut(s) 927
SfaNI GCATC 2 cut(s) 468, 649
SfcI CTRYAG 1 cut(s) 861
SfuI TTCGAA 1 cut(s) 70
SmiMI CAYNNNNRTG 2 cut(s) 464, 833
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SsiI CCGC 3 cut(s) 695, 826, 990
SspMI CTAG 1 cut(s) 831
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 606, 927
StyI CCWWGG 2 cut(s) 6, 586
TaaI ACNGT 8 cut(s) 381, 440, 496, 552, 616, 783, 802, 908
TaiI ACGT 1 cut(s) 1073
TaqI TCGA 9 cut(s) 70, 93, 291, 395, 526, 621, 915, 1052, 1251
TaqII GACCGA 1 cut(s) 264
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 4 cut(s) 392, 820, 968, 1288
Tru1I TTAA 4 cut(s) 767, 791, 1017, 1037
Tru9I TTAA 4 cut(s) 767, 791, 1017, 1037
TscAI CASTG 3 cut(s) 386, 822, 1107
TseFI GTSAC 7 cut(s) 468, 590, 637, 730, 931, 1066, 1141
TseI GCWGC 4 cut(s) 521, 867, 1083, 1264
Tsp45I GTSAC 7 cut(s) 468, 590, 637, 730, 931, 1066, 1141
TspDTI ATGAA 7 cut(s) 75, 288, 496, 553, 796, 956, 1122
TspGWI ACGGA 2 cut(s) 247, 834
TspRI CASTG 3 cut(s) 386, 822, 1107
Tth111I GACNNNGTC 1 cut(s) 635
XapI RAATTY 5 cut(s) 324, 856, 1046, 1060, 1222
XceI RCATGY 1 cut(s) 598
XspI CTAG 1 cut(s) 831
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.