Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010895

Physical Location & Seq

Genomic Coordinates Reverse (-)

8713248 .. 8718859

Gene Structure 5612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1121200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2934 bp

ATGCAATCAGGATATAACATAAATGATCTGTTTCAAGAAGCGCAAACCCGTTGGCTGAAGCCTGCTGAAGTGCTCTTCATATTGCAGAACCATGAAAAGTACAAGATCGCACCCGAACCCCCGCAACAGCCTAGTAGTGGATCTTTATTTCTTTTTAACAAGAGGATCCTTCGTTTCTTTCGTAGAGACGGGCACCAATGGCGTAAAAAGAAGGATGGAAGAACCGTAGGTGAAGCACATGAGCGACTTAAGGTTGGTAATGTCGAAACGTTAAATTGTTATTACGCACATGGAGAGGACAACCCAAGTTTTCAGAGACGTAGCTATTGGATGCTAGATCCGGCATATGAGCATATTGTACTTGTGCATTATAGAGAAACTAATGAGGGCAAGCCGAGTACTGGTTCTTTCGTACAATCACCAGTATCTTCTTCCTTTAGCCACAGTCCAAGCTCTAATTCTACTCCTCCTGGCTCTATCTCCATAGTTAGTGATTTACGTGAACCTAATCAGAATCTATCCAGTCCAGGTTCACTAGAAGTTAGTTCAGATATAGTCAACAAAAATGGAAGGGGGAACCCAGAAAATTTATATGGAACGGGGGAGTTGGATAGTTTGACTAAGTTTGATGTTAATCAAGCTTTGCGACAGCTTGAAGAGCAGTTAAGCTTAAATGAGGACAGCTTCAAAGGATTTATGGACGACAGTCCAAATGCTTCAGATATCCTGGATTATACTGATATCACCAATAAGGATCAGTTTGCTGCCTTCAATGAACCCGGATATGTTGTGCACGATCAGTTCTACAGTGAACCTGCTCGGATGCAAGGTAATGCAGACTATAGTGGTGAGCACTGTAAAATCATTGACCATGAATTCAGAGATGGAAACAAAGAATCTGCTACTTGGAAAGAGTTTCTGGATCCACGGAAGAATTCATCAGTCCTAAAGTCGGCGGAGAAATCTTTATATACATTGGACAGAAATGAAAACTCGCTGTCTTCCTTGAGGAGTGGACCAACTGAAGTGCAGGAGCATCATCAATGGCTAAATTCCAATGAAAATATTGTGGACAATGATTCTTTGCCACTGCCTCTAGAAGTTGACAGATCCAAACTGTATCCGTATGATTTTGTGTCTGGAACACATCCAGACTATTATACACAGTTATTTGAGCAAGGCCAGATAGGATCTGTTGATTCTGATATCAGTTTGACTGTTGCACAAAATCAAAAGTTCACTATTCGGGAAATATCTCCAGAGTGGGGTTATGCCACTGAAGCTACAAAGGTCGTCATTATTGGATCTTTTCTGTGTGATCCATCAGAATCTGCATGGACTTGTATGTTTGGTGATGTTGAAGTCCCTGCTCAGATCATTCAGGAAGGTGTAATCCGTTGTGAAGCTCCTCCTCACCTTCCTAGGAAGGTGACTGTCTGCCTTACTGCTGGAAATCGGGTGTCTTGCAGTGAAGTCAGGGAATTTGAGTATCGAGTTAAGAGCTGTAGCTGTAGTCACAATAATTCACCTCCACAAGAAGCTGCTGAGAGTGCTGAAGAGCTGTTATTACTAGTCAGATTTGTTCAGTTGCTCATGGATGATTCATCAACACAGGAAAGGGATAGTGTCGGATCTGATTCCCTGAGGAAATTGAAAGCTGACGATGATTCATGGAGTAGTATCATTGAAGCCTTGTTACTCGGCAGTGGAAGTTCATCTAGTACCATATATTGGCTTCTGGAAGAGCTACTGAAAGACAAGTTGCAGCAATGGCTTTCTTCCAGATCCCATGAATTTGACCAGAATGGTTGTTCCTTGTCCAAGAAAGAGCAAGGGATAATACACATGGTTGCTGGTTTGGGGTTTGCGTGGGCCTTAAACCCGATACTAAATTGTGGAGTCAATATAAACTTCCGCGACATAAACGGATGGACTGCTCTACATTGGACTGCTCGTTTTGGAAGGGAAAAGATGGCTGCCGTACTTTTAGCTTCTGGTGCATCAGCTGGAGCGGTGACAGATCCCAGTTCCCAAGATCCAGTTGGCAAAACTGCGGCATCTATTGCAGCAACCATTGGGCATAAGGGACTTGCAGGTTATCTTTCAGAGCTGTCACTGACTAGCCATCTGTCATCCCTTACGCTGGAAGAAAGTGAGTTTTCCAAAGGTTCAGCTGAGGTTGAAGCAGAATTAACAGTAAACAGCGTCTCAAATAGAAGCCTTGAGGGCAGTGAGGATCAGGTGTCACTTAAAAACACCTTGGCTGCAGTGCGGAATGCAGCTCAGGCTGCAGCACGCATACAGTCTGCGTTCCGGGCGCGTTCTTTCAGAAAACGGCAACATAAAGAAGCTGGTGTTAGCATAGATGACTACGGTATCAGTTCACGTGACATTCAAGGGCTTTCAGCGTTGTCAAGGCTAACCTTTCGCAATCCACGTGACTACAATTCAGCTGCAATATCTATTCAAAAGAAATACCGAGGGTATAAAGGCCGCAAAGATTTCCTCACTCTCCGCCAGAAAGTTGTGAAGATACAGGCGTATGTGCGAGGTTATCAGGTGAGGAAGCACTACAAGGTAATTTGCTGGGCTGTTGGAATTCTGGACAAGGTTGTACTAAGATGGCGAAGGAAAGGAGTTGGTTTGCGTGGCTTTCGGCAGGAGACAAAGTCGAAAGAAAGTGAAGATGAAGATATTCTCAAGGTGTTCCGCGAACAGAAAGTCAATGTAGCCATTGACGAGGCTGTCTCGCGAGTGCTGTCCATGGTTGAGTCTCCAGAGGCACGCCAGCAATATCATCGCATGCTGACACGGTACCATCAAGCTAAGGCTGAACTAGGAGGCACTAGCGGCGAAGGTGCCGAAGCAGATGCACCAAATTCCGGTGGCGATAACGTGATTGTAGACGACACTGATATGTACCTATTTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 CG-1 IQ TIG

Protein Analysis

978

Amino Acids

109.23

Weight (kDa)

5.74

Isoelectric Point (pI)

43.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	13 - 126	5.3e-49	CG-1 domain
TIG	PF01833	414 - 488	2.2e-07	IPT/TIG domain
Ank_2	PF12796	615 - 702	1.3e-06	Ankyrin repeats (3 copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67310
fragaria_vesca	FvH4_4g23551 FvH4_4g23551
malus_domestica	MD13G1121200.v1.1 MD16G1121200.v1.1
prunus_persica	Prupe.1G224000_v2.0.a1 Prupe.1G224000_v2.0.a1 Prupe.1G224000_v2.0.a1
pyrus_communis	pycom13g10550 pycom16g10390
rosa_chinensis	RchiOBHm_Chr4g0429961
rosa_laevigata	RLG00000007017
rosa_multiflora	Rmu_ssc0000150.1_g000038
rosa_roxburghii	Rroxscaffold_5G00371790
rosa_rugosa	Rorug04G0239700
rosa_samantha	Rh4AG296100 Rh4BG302600 Rh4CG318100 Rh4DG299900
rosa_wichuraiana	Rw4G025650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2744
Acc36I	ACCTGC	2 cut(s)	823, 2084
Acc65I	GGTACC	1 cut(s)	2814
AccB1I	GGYRCC	3 cut(s)	192, 2814, 2858
AccB7I	CCANNNNNTGG	1 cut(s)	1731
AccBSI	CCGCTC	1 cut(s)	2012
AccI	GTMKAC	1 cut(s)	2904
AccII	CGCG	4 cut(s)	1917, 2320, 2712, 2752
AclI	AACGTT	1 cut(s)	269
AcsI	RAATTY	8 cut(s)	586, 875, 934, 1051, 1481, 1793, 2598, 2878
AcuI	CTGAAG	6 cut(s)	77, 87, 702, 1044, 1299, 1575
AcvI	CACGTG	2 cut(s)	2387, 2438
AfaI	GTAC	9 cut(s)	101, 360, 400, 414, 1723, 1983, 2616, 2816, 2921
AfiI	CCNNNNNNNGG	7 cut(s)	137, 401, 952, 1265, 1731, 2143, 2882
AflII	CTTAAG	1 cut(s)	248
AhlI	ACTAGT	1 cut(s)	1570
AjnI	CCWGG	3 cut(s)	469, 526, 726
AjuI	GAANNNNNNNTTGG	2 cut(s)	442, 474
Alw21I	GWGCWC	3 cut(s)	75, 795, 855
Alw26I	GTCTC	6 cut(s)	180, 310, 2212, 2657, 2752, 2778
Alw44I	GTGCAC	1 cut(s)	791
AlwNI	CAGNNNCTG	1 cut(s)	1331
AoxI	GGCC	3 cut(s)	1180, 1872, 2491
ApaLI	GTGCAC	1 cut(s)	791
ApoI	RAATTY	8 cut(s)	586, 875, 934, 1051, 1481, 1793, 2598, 2878
ArsI	GACNNNNNNTTYG	2 cut(s)	2706, 2738
Asp700I	GAANNNNTTC	1 cut(s)	2694
Asp718I	GGTACC	1 cut(s)	2814
AspA2I	CCTAGG	1 cut(s)	1421
AspLEI	GCGC	2 cut(s)	43, 2320
AspS9I	GGNCC	2 cut(s)	1016, 1872
AsuC2I	CCSGG	2 cut(s)	780, 2315
AvaII	GGWCC	1 cut(s)	1016
AvrII	CCTAGG	1 cut(s)	1421
AxyI	CCTNAGG	1 cut(s)	1643
BaeGI	GKGCMC	2 cut(s)	195, 795
BaeI	ACNNNNGTAYC	4 cut(s)	2359, 2392, 2525, 2558
BamHI	GGATCC	2 cut(s)	165, 922
BanI	GGYRCC	3 cut(s)	192, 2814, 2858
BarI	GAAGNNNNNNTAC	2 cut(s)	1680, 1712
BbrPI	CACGTG	2 cut(s)	2387, 2438
BbsI	GAAGAC	1 cut(s)	993
Bbv12I	GWGCWC	3 cut(s)	75, 795, 855
BbvCI	CCTCAGC	1 cut(s)	2175
BccI	CCATC	8 cut(s)	209, 878, 1330, 1923, 1966, 2133, 2616, 2826
BceAI	ACGGC	2 cut(s)	1964, 2351
BcgI	CGANNNNNNTGC	4 cut(s)	2780, 2814, 2852, 2886
BciT130I	CCWGG	3 cut(s)	471, 528, 728
BciVI	GTATCC	1 cut(s)	1131
BcnI	CCSGG	2 cut(s)	780, 2315
BcoDI	GTCTC	6 cut(s)	180, 310, 2212, 2657, 2752, 2778
BcuI	ACTAGT	1 cut(s)	1570
BfmI	CTRYAG	6 cut(s)	805, 841, 1504, 1510, 2265, 2289
BfrI	CTTAAG	1 cut(s)	248
BfuAI	ACCTGC	2 cut(s)	823, 2084
BfuI	GTATCC	1 cut(s)	1131
BglI	GCCNNNNNGGC	1 cut(s)	2226
BlnI	CCTAGG	1 cut(s)	1421
BmcAI	AGTACT	1 cut(s)	400
Bme1390I	CCNGG	5 cut(s)	471, 528, 728, 780, 2315
Bme18I	GGWCC	1 cut(s)	1016
BmgT120I	GGNCC	2 cut(s)	1016, 1872
BmiI	GGNNCC	6 cut(s)	167, 194, 578, 924, 2816, 2860
BmrFI	CCNGG	5 cut(s)	471, 528, 728, 780, 2315
BmrI	ACTGGG	1 cut(s)	2019
BmsI	GCATC	6 cut(s)	321, 813, 1045, 2009, 2066, 2860
BmuI	ACTGGG	1 cut(s)	2019
BoxI	GACNNNNGTC	1 cut(s)	705
BpiI	GAAGAC	1 cut(s)	993
BplI	GAGNNNNNCTC	2 cut(s)	2732, 2764
BpmI	CTGGAG	3 cut(s)	1242, 2028, 2760
Bpu10I	CCTNAGC	3 cut(s)	2175, 2283, 2826
BpuEI	CTTGAG	3 cut(s)	1027, 2243, 2684
BpuMI	CCSGG	2 cut(s)	780, 2315
BsaAI	YACGTR	3 cut(s)	500, 2387, 2438
BsaBI	GATNNNNATC	1 cut(s)	633
BsaJI	CCNNGG	5 cut(s)	926, 1421, 2259, 2479, 2763
BsaWI	WCCGGW	1 cut(s)	2882
BsaXI	ACNNNNNCTCC	2 cut(s)	2654, 2684
Bsc4I	CCNNNNNNNGG	7 cut(s)	137, 401, 952, 1265, 1731, 2143, 2882
Bse1I	ACTGG	5 cut(s)	406, 422, 522, 2025, 2039
Bse21I	CCTNAGG	1 cut(s)	1643
Bse3DI	GCAATG	1 cut(s)	1775
Bse8I	GATNNNNATC	1 cut(s)	633
BseBI	CCWGG	3 cut(s)	471, 528, 728
BseDI	CCNNGG	5 cut(s)	926, 1421, 2259, 2479, 2763
BseGI	GGATG	7 cut(s)	220, 336, 828, 1147, 1603, 1934, 2132
BseJI	GATNNNNATC	1 cut(s)	633
BseLI	CCNNNNNNNGG	7 cut(s)	137, 401, 952, 1265, 1731, 2143, 2882
BseMI	GCAATG	1 cut(s)	1775
BseMII	CTCAG	5 cut(s)	1385, 1536, 1634, 2166, 2297
BseNI	ACTGG	5 cut(s)	406, 422, 522, 2025, 2039
BseRI	GAGGAG	4 cut(s)	456, 1024, 1398, 1401
BseSI	GKGCMC	2 cut(s)	195, 795
BseYI	CCCAGC	1 cut(s)	2586
BsgI	GTGCAG	1 cut(s)	1049
Bsh1236I	CGCG	4 cut(s)	1917, 2320, 2712, 2752
BshFI	GGCC	3 cut(s)	1182, 1874, 2493
BshNI	GGYRCC	3 cut(s)	192, 2814, 2858
BsiHKAI	GWGCWC	3 cut(s)	75, 795, 855
BsiSI	CCGG	4 cut(s)	341, 780, 2314, 2883
BslFI	GGGAC	2 cut(s)	1349, 2100
BslI	CCNNNNNNNGG	7 cut(s)	137, 401, 952, 1265, 1731, 2143, 2882
BsmAI	GTCTC	6 cut(s)	180, 310, 2212, 2657, 2752, 2778
BsmBI	CGTCTC	3 cut(s)	180, 310, 2212
BsmFI	GGGAC	2 cut(s)	1349, 2100
BsmI	GAATGC	1 cut(s)	2281
BsnI	GGCC	3 cut(s)	1182, 1874, 2493
Bsp1286I	GDGCHC	4 cut(s)	75, 195, 795, 855
Bsp19I	CCATGG	1 cut(s)	2763
Bsp68I	TCGCGA	1 cut(s)	2752
BspANI	GGCC	3 cut(s)	1182, 1874, 2493
BspCNI	CTCAG	5 cut(s)	1384, 1537, 1635, 2167, 2296
BspFNI	CGCG	4 cut(s)	1917, 2320, 2712, 2752
BspLI	GGNNCC	6 cut(s)	167, 194, 578, 924, 2816, 2860
BspMAI	CTGCAG	2 cut(s)	2269, 2293
BspMI	ACCTGC	2 cut(s)	823, 2084
BspQI	GCTCTTC	4 cut(s)	80, 651, 1551, 1737
BspT107I	GGYRCC	3 cut(s)	192, 2814, 2858
BspTI	CTTAAG	1 cut(s)	248
BsrBI	CCGCTC	1 cut(s)	2012
BsrDI	GCAATG	1 cut(s)	1775
BsrI	ACTGG	5 cut(s)	406, 422, 522, 2025, 2039
BssECI	CCNNGG	5 cut(s)	926, 1421, 2259, 2479, 2763
BssT1I	CCWWGG	3 cut(s)	1421, 2259, 2763
Bst2UI	CCWGG	3 cut(s)	471, 528, 728
Bst6I	CTCTTC	4 cut(s)	80, 651, 1551, 1737
BstAFI	CTTAAG	1 cut(s)	248
BstAPI	GCANNNNNTGC	1 cut(s)	2063
BstBAI	YACGTR	3 cut(s)	500, 2387, 2438
BstC8I	GCNNGC	6 cut(s)	63, 392, 2296, 2785, 2789, 2804
BstDEI	CTNAG	8 cut(s)	621, 1371, 1545, 1643, 2175, 2283, 2618, 2826
BstDSI	CCRYGG	2 cut(s)	926, 2763
BstF5I	GGATG	7 cut(s)	220, 336, 828, 1147, 1603, 1934, 2132
BstFNI	CGCG	4 cut(s)	1917, 2320, 2712, 2752
BstHHI	GCGC	2 cut(s)	43, 2320
BstMAI	GTCTC	6 cut(s)	180, 310, 2212, 2657, 2752, 2778
BstNI	CCWGG	3 cut(s)	471, 528, 728
BstNSI	RCATGY	1 cut(s)	2806
BstPAI	GACNNNNGTC	1 cut(s)	705
BstSCI	CCNGG	5 cut(s)	469, 526, 726, 778, 2313
BstSFI	CTRYAG	6 cut(s)	805, 841, 1504, 1510, 2265, 2289
BstSLI	GKGCMC	2 cut(s)	195, 795
BstUI	CGCG	4 cut(s)	1917, 2320, 2712, 2752
BstV2I	GAAGAC	1 cut(s)	993
Bsu36I	CCTNAGG	1 cut(s)	1643
BsuI	GTATCC	1 cut(s)	1131
BsuRI	GGCC	3 cut(s)	1182, 1874, 2493
BtgI	CCRYGG	2 cut(s)	926, 2763
BtgZI	GCGATG	1 cut(s)	2783
BtsCI	GGATG	7 cut(s)	220, 336, 828, 1147, 1603, 1934, 2132
BtsI	GCAGTG	5 cut(s)	1088, 1474, 1711, 2236, 2274
BtuMI	TCGCGA	1 cut(s)	2752
BveI	ACCTGC	2 cut(s)	823, 2084
Cac8I	GCNNGC	6 cut(s)	63, 392, 2296, 2785, 2789, 2804
CaiI	CAGNNNCTG	1 cut(s)	1331
CfoI	GCGC	2 cut(s)	43, 2320
Cfr13I	GGNCC	2 cut(s)	1016, 1872
CseI	GACGC	1 cut(s)	2194
Csp6I	GTAC	9 cut(s)	100, 359, 399, 413, 1722, 1982, 2615, 2815, 2920
CviQI	GTAC	9 cut(s)	100, 359, 399, 413, 1722, 1982, 2615, 2815, 2920
DdeI	CTNAG	8 cut(s)	621, 1371, 1545, 1643, 2175, 2283, 2618, 2826
DrdI	GACNNNNNNGTC	1 cut(s)	2744
DseDI	GACNNNNNNGTC	1 cut(s)	2744
Eam1104I	CTCTTC	4 cut(s)	80, 651, 1551, 1737
EarI	CTCTTC	4 cut(s)	80, 651, 1551, 1737
EciI	GGCGGA	2 cut(s)	971, 2504
Eco130I	CCWWGG	3 cut(s)	1421, 2259, 2763
Eco32I	GATATC	3 cut(s)	724, 742, 1207
Eco47I	GGWCC	1 cut(s)	1016
Eco57I	CTGAAG	6 cut(s)	77, 87, 702, 1044, 1299, 1575
Eco72I	CACGTG	2 cut(s)	2387, 2438
Eco81I	CCTNAGG	1 cut(s)	1643
EcoRI	GAATTC	3 cut(s)	875, 934, 2598
EcoRII	CCWGG	3 cut(s)	469, 526, 726
EcoRV	GATATC	3 cut(s)	724, 742, 1207
EcoT14I	CCWWGG	3 cut(s)	1421, 2259, 2763
ErhI	CCWWGG	3 cut(s)	1421, 2259, 2763
Esp3I	CGTCTC	3 cut(s)	180, 310, 2212
FaqI	GGGAC	2 cut(s)	1349, 2100
FauI	CCCGC	1 cut(s)	129
FauNDI	CATATG	1 cut(s)	346
FblI	GTMKAC	1 cut(s)	2904
FokI	GGATG	7 cut(s)	227, 343, 835, 1134, 1610, 1941, 2119
GlaI	GCGC	2 cut(s)	42, 2319
GsaI	CCCAGC	1 cut(s)	2590
GsuI	CTGGAG	3 cut(s)	1242, 2028, 2760
HaeIII	GGCC	3 cut(s)	1182, 1874, 2493
HapII	CCGG	4 cut(s)	341, 780, 2314, 2883
HgaI	GACGC	1 cut(s)	2194
HhaI	GCGC	2 cut(s)	43, 2320
Hin6I	GCGC	2 cut(s)	41, 2318
HinP1I	GCGC	2 cut(s)	41, 2318
HincII	GTYRAC	2 cut(s)	559, 1105
HindII	GTYRAC	2 cut(s)	559, 1105
HindIII	AAGCTT	2 cut(s)	639, 667
HpaII	CCGG	4 cut(s)	341, 780, 2314, 2883
HpyCH4IV	ACGT	6 cut(s)	269, 319, 499, 2386, 2437, 2895
HpyF3I	CTNAG	8 cut(s)	621, 1371, 1545, 1643, 2175, 2283, 2618, 2826
HpySE526I	ACGT	6 cut(s)	269, 319, 499, 2386, 2437, 2895
HspAI	GCGC	2 cut(s)	41, 2318
KpnI	GGTACC	1 cut(s)	2818
LguI	GCTCTTC	4 cut(s)	80, 651, 1551, 1737
LmnI	GCTCC	3 cut(s)	1033, 1411, 2009
LweI	GCATC	6 cut(s)	321, 813, 1045, 2009, 2066, 2860
MaeII	ACGT	6 cut(s)	269, 319, 499, 2386, 2437, 2895
MaeIII	GTNAC	8 cut(s)	1429, 1514, 1695, 2014, 2112, 2244, 2387, 2438
MbiI	CCGCTC	1 cut(s)	2012
MhlI	GDGCHC	4 cut(s)	75, 195, 795, 855
MlyI	GAGTC	2 cut(s)	1908, 2780
MmeI	TCCRAC	3 cut(s)	588, 1609, 2575
MroXI	GAANNNNTTC	1 cut(s)	2694
MslI	CAYNNNNRTG	1 cut(s)	2915
MspA1I	CMGCKG	3 cut(s)	2006, 2174, 2453
MspCI	CTTAAG	1 cut(s)	248
MspI	CCGG	4 cut(s)	341, 780, 2314, 2883
MspR9I	CCNGG	5 cut(s)	471, 528, 728, 780, 2315
Mva1269I	GAATGC	1 cut(s)	2281
MvaI	CCWGG	3 cut(s)	471, 528, 728
MvnI	CGCG	4 cut(s)	1917, 2320, 2712, 2752
NciI	CCSGG	2 cut(s)	780, 2315
NcoI	CCATGG	1 cut(s)	2763
NdeI	CATATG	1 cut(s)	346
NlaIV	GGNNCC	6 cut(s)	167, 194, 578, 924, 2816, 2860
NmeAIII	GCCGAG	2 cut(s)	420, 1680
NmuCI	GTSAC	7 cut(s)	1429, 1514, 2014, 2112, 2244, 2387, 2438
NruI	TCGCGA	1 cut(s)	2752
NspI	RCATGY	1 cut(s)	2806
PaeI	GCATGC	1 cut(s)	2806
PciSI	GCTCTTC	4 cut(s)	80, 651, 1551, 1737
PcsI	WCGNNNNNNNCGW	2 cut(s)	178, 2434
PctI	GAATGC	1 cut(s)	2281
PdmI	GAANNNNTTC	1 cut(s)	2694
PfeI	GAWTC	8 cut(s)	514, 896, 1079, 1199, 1328, 1601, 1637, 1667
PflFI	GACNNNGTC	1 cut(s)	2668
PflMI	CCANNNNNTGG	1 cut(s)	1731
PfoI	TCCNGGA	1 cut(s)	726
PleI	GAGTC	2 cut(s)	1907, 2779
PmaCI	CACGTG	2 cut(s)	2387, 2438
PmlI	CACGTG	2 cut(s)	2387, 2438
PpsI	GAGTC	2 cut(s)	1907, 2779
Ppu21I	YACGTR	3 cut(s)	500, 2387, 2438
PshAI	GACNNNNGTC	1 cut(s)	705
Psp1406I	AACGTT	1 cut(s)	269
Psp6I	CCWGG	3 cut(s)	469, 526, 726
PspCI	CACGTG	2 cut(s)	2387, 2438
PspFI	CCCAGC	1 cut(s)	2586
PspGI	CCWGG	3 cut(s)	469, 526, 726
PspN4I	GGNNCC	6 cut(s)	167, 194, 578, 924, 2816, 2860
PspPI	GGNCC	2 cut(s)	1016, 1872
PstI	CTGCAG	2 cut(s)	2269, 2293
PstNI	CAGNNNCTG	1 cut(s)	1331
PsyI	GACNNNGTC	1 cut(s)	2668
PvuII	CAGCTG	3 cut(s)	2006, 2174, 2453
RruI	TCGCGA	1 cut(s)	2752
RsaI	GTAC	9 cut(s)	101, 360, 400, 414, 1723, 1983, 2616, 2816, 2921
RsaNI	GTAC	9 cut(s)	100, 359, 399, 413, 1722, 1982, 2615, 2815, 2920
RseI	CAYNNNNRTG	1 cut(s)	2915
SapI	GCTCTTC	4 cut(s)	80, 651, 1551, 1737
Sau96I	GGNCC	2 cut(s)	1016, 1872
ScaI	AGTACT	1 cut(s)	400
SchI	GAGTC	2 cut(s)	1908, 2780
ScrFI	CCNGG	5 cut(s)	471, 528, 728, 780, 2315
SduI	GDGCHC	4 cut(s)	75, 195, 795, 855
SfaNI	GCATC	6 cut(s)	321, 813, 1045, 2009, 2066, 2860
SfcI	CTRYAG	6 cut(s)	805, 841, 1504, 1510, 2265, 2289
SinI	GGWCC	1 cut(s)	1016
SmiMI	CAYNNNNRTG	1 cut(s)	2915
SmlI	CTYRAG	4 cut(s)	248, 1006, 2222, 2699
SmoI	CTYRAG	4 cut(s)	248, 1006, 2222, 2699
SpeI	ACTAGT	1 cut(s)	1570
SphI	GCATGC	1 cut(s)	2806
SspI	AATATT	1 cut(s)	1066
StyD4I	CCNGG	5 cut(s)	469, 526, 726, 778, 2313
StyI	CCWWGG	3 cut(s)	1421, 2259, 2763
TaiI	ACGT	6 cut(s)	272, 322, 502, 2389, 2440, 2898
TaqI	TCGA	3 cut(s)	264, 1492, 2672
TatI	WGTACW	4 cut(s)	99, 358, 398, 2614
TauI	GCSGC	3 cut(s)	2057, 2496, 2853
TfiI	GAWTC	8 cut(s)	514, 896, 1079, 1199, 1328, 1601, 1637, 1667
TseFI	GTSAC	7 cut(s)	1429, 1514, 2014, 2112, 2244, 2387, 2438
Tsp45I	GTSAC	7 cut(s)	1429, 1514, 2014, 2112, 2244, 2387, 2438
TspGWI	ACGGA	4 cut(s)	943, 1113, 1385, 1941
Tth111I	GACNNNGTC	1 cut(s)	2668
Van91I	CCANNNNNTGG	1 cut(s)	1731
Vha464I	CTTAAG	1 cut(s)	248
VneI	GTGCAC	1 cut(s)	791
VpaK11BI	GGWCC	1 cut(s)	1016
XapI	RAATTY	8 cut(s)	586, 875, 934, 1051, 1481, 1793, 2598, 2878
XbaI	TCTAGA	1 cut(s)	1096
XceI	RCATGY	1 cut(s)	2806
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2039
XmaJI	CCTAGG	1 cut(s)	1421
XmiI	GTMKAC	1 cut(s)	2904
XmnI	GAANNNNTTC	1 cut(s)	2694
ZrmI	AGTACT	1 cut(s)	400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.