Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006328

Physical Location & Seq

Genomic Coordinates Reverse (-)

12639580 .. 12646509

Gene Structure 6930 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1157900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGATGGAGAATCAGCTCGTGGGCTCAGAGATTCATGGATTCCATACAATGCAAGATTTGGATGTTCCGACTATTATGGAAGAAGCCAAATCAAGATGGCTCCGCCCAAATGAAATTCATGCAATACTTTACAATCACAAGTATTTCACCATCCACGTCAAGCCAGTGAACTTGCCCAAAAGTGGCACCATTGTACTGTTTGATCGGAAGATGCTTAGAAACTTCAGGAAAGATGGCCATAACTGGAAGAAGAAAAAGGATGGGAAGACAGTTAAAGAAGCTCATGAACATTTGAAAGTTGGCAATGAAGAAAGAGTTCATGTATACTATGCACATGGTGAAGATAGCCCAACCTTTGTCCGGAGGTGTTATTGGTTACTTGATAAGAGTCTAGAACATATAGTACTTGTTCATTACCGTGAAACACAAGAGGTTCAGGGTTCTCCGGTCACACCTGTGAATTCAAACTCTAGTTCTGTCTCTGACCCATCTGCTCCGTGGTTTTTATCAGAAGAACTTGATTCTGGGGCTAACAACGCATATTGTGCTGGTGAAAATGAGCTTCCAGAGCTTGGTGACAGGTTGACTGTTAATAATCATGAACAGAGGCTCCATGATATTAATACACTTGAATGGGAGGAACTTCTGGTTACAAATGATTCTAAAGAAAACAACATTTCAGGCTATGACCAGCAGAATCAAGTCGTGGGAAATGGCTCCAATAGTGGTGGAGCCAGCAATTTATCAGCTGAAATTTCTTCTATTGGTAATTTAACAAATCCAACCACGGAGAGTGAAAGCATTCCGTTCAATCTTCCAGGCAGTCATGTTGAGACTGTGGGGGGTGAAGTGAATTCAAATGCCCAGAGAAGGGATTCTATTGCCAAGGAAGACAAGACAGTTTTGGATACTAATGGATTACATAGTCAAGACAGTTTTGGAAGGTGGATAAACGAGATCATGACAGACTCCCAACCTTCAGTGGAAGACCCAGTACTTGGTTCCTCACTTTCAGCTGCGCAAAATTCATTTGCTTCTCCCGCTATGGATCATCTTCAATCTTCTGTTCCTCAGCAAATATTTAACATAACTGATATTTCGCCTACATGGGCTTTTTCAAACGAAAAAACAAAGATTCTAATCACTGGCTTCTTACATAAAGAGTTTTTTCATCTTGCAAAGTCAGATCTACTTTGTGTTTGTGGTGATGTATGTCTTCGTGCGGAAATTATTCAGACCGGGGTGTATCGCTGTTTTGTACCTCCACATTTGCCCAGAATAGTAAACCTTTTTATGAGTATTGATGGCCATAAACCCATCAGCCAAGTACTAAACTTTGAATATCGTGCTCCTGTTTTGAGTGATACAGTAGTTTCTTCAGAAGATAACAAGTGGGAAGAATTCCAAGTACAAATGCGGCTTGCTTATTTGCTTTTCTCCTCATCTAAGAGCCTTAACATTATATCTAATAAAGTGCCCCCGAATGCCCTGAAAGAAGCCAAGAAGTTTTCCCACAGAACCTCTCACATATCTAATAGCTGGCCATACTTGATGAAGGCAATTGAAGAGAACAAAACTCCCTTACCGCTGGCAAAGGATAGTCTGTTTGAACTAATTTTGAGGAACAGGCTAAAGGATTGGCTGTTGGAGAAAGTTGTTGAGGTCTCTAAAACTAAAGAATATGATGCTCATGGTCAAGGAGTAATCCATTTATGTGCTATTCTAGAATATACATGGGCTGTTCGCTTGTTTTCATGGTCTGGCTTGTCATTAGACTTTCGTGATAAACGTGGATGGACGGCCCTTCATTGGGCGGCATATTGTGGAAGGGAGAAAATGGTTGCAGTACTTTTATCTGCCGGGGCAAAGCCAAACTTGGTCACAGACCCCACGAAAGATAACCCTGGCGGATCCAATGCTGCTGATCTTGCATTCATGAAGGGCCATGATGGCTTAGCAGCTTATCTTTCAGAGAAGGCTTTGGTAGAACAATTCAAGGATATGCGCATGGCTGGAAATGCTAGTGGCTCTCTTGAAATTCGCTCAAACTATGCAGCAAACTCTGAGAACCTCAGTGAGGAAGAGCTATATCTGAAGGATACTTTGGCAGCTTATCGGACAGCTGCTGATGCAGCAGCACGAATACAGGCTGCCTTCAGGGAGAACTCACTTAAACAAAAGACTAAAGCGATTCAGTATTCCATTCCAGAGGCTGAAGCACGTGGTATTATTGCAGCACTGAAAATTCAACATGCCTTTAGAAACTATGAGGCAAAAAAGAAGATCAAAGCTGCTGCTCACATTCAATATAGGTTTCGTACTTGGAAGATGCGTCAAGAATTTCTTAGTTTGCGTCGTCAAGCTATCAAAATTCAAGCTGCTTTTCGGGGCTTCCAAGTGCGGAGGCAGTACCGTAAAGTTCTCTGGTCAGTTGGAGTGCTTGAGAAAGCTGTTTTGAGATGGCGCTTCAAAAGAAGAGGGCTTCGTGGGCTTAAGGTGGCCCCAGCTGAAGTCAGTGCAGATAAGAATCCGGAAAGTGATACAGAAGAAGACTTCTACAGGGCCAGCCGGAAACAAGCTGAAGAGCGTATTGAAAGATCTGTTGTACGTGTTCAGGCCATGTTCCGCTCAAAGAAAGCACAACAAGAATATAGTAGGATGAAGATGGCCCACAATGAAGCAACGCTGGAATTTGATGATTTTCTTGATCCTGATGCAATCATGGATAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 CG-1 IQ TIG

Protein Analysis

911

Amino Acids

103.17

Weight (kDa)

7.42

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	28 - 141	6.3e-46	CG-1 domain
Ank_2	PF12796	559 - 626	1.5e-07	Ankyrin repeats (3 copies)
Ank_2	PF12796	570 - 659	1.5e-07	Ankyrin repeats (3 copies)
Ank	PF00023	598 - 628	3.1e-07	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16940 AT3G16940 AT3G16940
fragaria_vesca	FvH4_4g19400 FvH4_4g19400
malus_domestica	MD16G1157900.v1.1
prunus_persica	Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1
pyrus_communis	pycom13g13450 pycom16g13290
rosa_chinensis	RchiOBHm_Chr4g0424431
rosa_laevigata	RLG00000007475
rosa_multiflora	Rmu_sc0003567.1_g000011
rosa_roxburghii	Rroxscaffold_5G00366370
rosa_rugosa	Rorug04G0197700 Rorug04G0197800
rosa_samantha	Rh4AG253800 Rh4BG259100 Rh4CG270400 Rh4DG253600
rosa_wichuraiana	Rw4G021920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1020, 2006
AccB1I	GGYRCC	1 cut(s)	185
AccB7I	CCANNNNNTGG	2 cut(s)	572, 998
AccBSI	CCGCTC	1 cut(s)	2628
AccI	GTMKAC	1 cut(s)	324
AccIII	TCCGGA	2 cut(s)	360, 2530
AciI	CCGC	9 cut(s)	103, 1041, 1223, 1417, 1586, 1814, 1908, 2401, 2626
AclWI	GGATC	4 cut(s)	1056, 1905, 1918, 2702
AcoI	YGGCCR	3 cut(s)	235, 1306, 1541
AcuI	CTGAAG	8 cut(s)	208, 963, 1362, 2114, 2140, 2235, 2529, 2601
AcvI	CACGTG	1 cut(s)	2222
AdeI	CACNNNGTG	1 cut(s)	338
AfiI	CCNNNNNNNGG	9 cut(s)	182, 360, 572, 870, 871, 998, 1809, 1810, 2077
AflII	CTTAAG	1 cut(s)	2492
AflIII	ACRYGT	1 cut(s)	2608
AjiI	CACGTC	1 cut(s)	157
AjnI	CCWGG	2 cut(s)	817, 1903
AjuI	GAANNNNNNNTTGG	2 cut(s)	2087, 2119
AleI	CACNNNNGTG	1 cut(s)	455
AloI	GAACNNNNNNTCC	2 cut(s)	594, 626
Alw21I	GWGCWC	1 cut(s)	1351
Alw26I	GTCTC	3 cut(s)	484, 827, 1669
AlwI	GGATC	4 cut(s)	1056, 1905, 1918, 2702
AlwNI	CAGNNNCTG	2 cut(s)	2126, 2213
Aor13HI	TCCGGA	2 cut(s)	360, 2530
AoxI	GGCC	9 cut(s)	235, 1306, 1541, 1800, 1942, 2499, 2562, 2616, 2667
AseI	ATTAAT	1 cut(s)	621
Asp700I	GAANNNNTTC	4 cut(s)	315, 801, 874, 1230
AspLEI	GCGC	3 cut(s)	1021, 2007, 2466
AspS9I	GGNCC	5 cut(s)	1801, 1942, 2500, 2562, 2668
AsuC2I	CCSGG	2 cut(s)	1240, 1861
AsuHPI	GGTGA	6 cut(s)	139, 350, 563, 587, 857, 1217
BaeGI	GKGCMC	1 cut(s)	1479
BaeI	ACNNNNGTAYC	2 cut(s)	1356, 1389
BalI	TGGCCA	3 cut(s)	237, 1308, 1543
BamHI	GGATCC	1 cut(s)	1910
BanI	GGYRCC	1 cut(s)	185
BanII	GRGCYC	1 cut(s)	26
BarI	GAAGNNNNNNTAC	2 cut(s)	978, 1010
BauI	CACGAG	1 cut(s)	17
BbrPI	CACGTG	1 cut(s)	2222
BbsI	GAAGAC	5 cut(s)	272, 897, 993, 1208, 2556
Bbv12I	GWGCWC	1 cut(s)	1351
BbvCI	CCTCAGC	1 cut(s)	1071
BceAI	ACGGC	1 cut(s)	1815
BciT130I	CCWGG	2 cut(s)	819, 1905
BciVI	GTATCC	2 cut(s)	901, 2092
BcnI	CCSGG	2 cut(s)	1240, 1861
BcoDI	GTCTC	3 cut(s)	484, 827, 1669
BfaI	CTAG	4 cut(s)	392, 471, 1724, 2022
BfmI	CTRYAG	1 cut(s)	2557
BfoI	RGCGCY	1 cut(s)	2467
BfrI	CTTAAG	1 cut(s)	2492
BfuI	GTATCC	2 cut(s)	901, 2092
BglI	GCCNNNNNGGC	1 cut(s)	1950
BglII	AGATCT	2 cut(s)	1186, 2597
BlpI	GCTNAGC	1 cut(s)	1954
BmcAI	AGTACT	4 cut(s)	405, 996, 1329, 1848
Bme1390I	CCNGG	4 cut(s)	819, 1240, 1861, 1905
BmgBI	CACGTC	1 cut(s)	157
BmgT120I	GGNCC	5 cut(s)	1801, 1942, 2500, 2562, 2668
BmiI	GGNNCC	8 cut(s)	101, 187, 611, 718, 733, 1003, 1912, 2502
BmrFI	CCNGG	4 cut(s)	819, 1240, 1861, 1905
BmrI	ACTGGG	1 cut(s)	986
BmsI	GCATC	5 cut(s)	201, 1675, 2119, 2319, 2704
BmuI	ACTGGG	1 cut(s)	986
BpiI	GAAGAC	5 cut(s)	272, 897, 993, 1208, 2556
Bpu10I	CCTNAGC	1 cut(s)	1071
Bpu1102I	GCTNAGC	1 cut(s)	1954
BpuEI	CTTGAG	1 cut(s)	2462
BpuMI	CCSGG	2 cut(s)	1240, 1861
BsaAI	YACGTR	2 cut(s)	2222, 2609
BsaBI	GATNNNNATC	2 cut(s)	1139, 2526
BsaI	GGTCTC	1 cut(s)	1669
BsaJI	CCNNGG	6 cut(s)	497, 786, 885, 1239, 1860, 1903
BsaWI	WCCGGW	3 cut(s)	360, 445, 2530
BsaXI	ACNNNNNCTCC	2 cut(s)	594, 624
Bsc4I	CCNNNNNNNGG	9 cut(s)	182, 360, 572, 870, 871, 998, 1809, 1810, 2077
Bse1I	ACTGG	4 cut(s)	164, 248, 992, 1150
Bse3DI	GCAATG	1 cut(s)	310
Bse8I	GATNNNNATC	2 cut(s)	1139, 2526
BseAI	TCCGGA	2 cut(s)	360, 2530
BseBI	CCWGG	2 cut(s)	819, 1905
BseDI	CCNNGG	6 cut(s)	497, 786, 885, 1239, 1860, 1903
BseGI	GGATG	5 cut(s)	67, 150, 265, 1799, 2664
BseJI	GATNNNNATC	2 cut(s)	1139, 2526
BseLI	CCNNNNNNNGG	9 cut(s)	182, 360, 572, 870, 871, 998, 1809, 1810, 2077
BseMI	GCAATG	1 cut(s)	310
BseMII	CTCAG	4 cut(s)	39, 1085, 2055, 2086
BseNI	ACTGG	4 cut(s)	164, 248, 992, 1150
BseRI	GAGGAG	1 cut(s)	1429
BseSI	GKGCMC	1 cut(s)	1479
BseYI	CCCAGC	1 cut(s)	2503
BsgI	GTGCAG	1 cut(s)	2538
BshFI	GGCC	9 cut(s)	237, 1308, 1543, 1802, 1944, 2501, 2564, 2618, 2669
BshNI	GGYRCC	1 cut(s)	185
BsiHKAI	GWGCWC	1 cut(s)	1351
BsiSI	CCGG	6 cut(s)	361, 446, 1239, 1860, 2531, 2569
BslI	CCNNNNNNNGG	9 cut(s)	182, 360, 572, 870, 871, 998, 1809, 1810, 2077
BsmAI	GTCTC	3 cut(s)	484, 827, 1669
BsmI	GAATGC	3 cut(s)	801, 1489, 1931
BsnI	GGCC	9 cut(s)	237, 1308, 1543, 1802, 1944, 2501, 2564, 2618, 2669
Bso31I	GGTCTC	1 cut(s)	1669
Bsp1286I	GDGCHC	3 cut(s)	26, 1351, 1479
Bsp13I	TCCGGA	2 cut(s)	360, 2530
Bsp143I	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
Bsp1720I	GCTNAGC	1 cut(s)	1954
BspACI	CCGC	9 cut(s)	103, 1041, 1223, 1417, 1586, 1814, 1908, 2401, 2626
BspANI	GGCC	9 cut(s)	237, 1308, 1543, 1802, 1944, 2501, 2564, 2618, 2669
BspCNI	CTCAG	4 cut(s)	38, 1084, 2056, 2085
BspEI	TCCGGA	2 cut(s)	360, 2530
BspHI	TCATGA	4 cut(s)	283, 598, 960, 1935
BspLI	GGNNCC	8 cut(s)	101, 187, 611, 718, 733, 1003, 1912, 2502
BspPI	GGATC	4 cut(s)	1056, 1905, 1918, 2702
BspQI	GCTCTTC	2 cut(s)	2076, 2577
BspT107I	GGYRCC	1 cut(s)	185
BspTI	CTTAAG	1 cut(s)	2492
BspTNI	GGTCTC	1 cut(s)	1669
BsrBI	CCGCTC	1 cut(s)	2628
BsrDI	GCAATG	1 cut(s)	310
BsrI	ACTGG	4 cut(s)	164, 248, 992, 1150
BssECI	CCNNGG	6 cut(s)	497, 786, 885, 1239, 1860, 1903
BssMI	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
BssNAI	GTATAC	1 cut(s)	325
BssSI	CACGAG	1 cut(s)	17
BssT1I	CCWWGG	1 cut(s)	885
Bst1107I	GTATAC	1 cut(s)	325
Bst2BI	CACGAG	1 cut(s)	17
Bst2UI	CCWGG	2 cut(s)	819, 1905
Bst4CI	ACNGT	9 cut(s)	198, 271, 419, 589, 838, 901, 935, 1369, 2414
Bst6I	CTCTTC	4 cut(s)	1560, 2076, 2470, 2577
BstAFI	CTTAAG	1 cut(s)	2492
BstAPI	GCANNNNNTGC	1 cut(s)	545
BstBAI	YACGTR	2 cut(s)	2222, 2609
BstC8I	GCNNGC	5 cut(s)	736, 1422, 1541, 1590, 2566
BstDEI	CTNAG	8 cut(s)	25, 215, 1071, 1446, 1954, 2064, 2072, 2345
BstDSI	CCRYGG	2 cut(s)	497, 786
BstENI	CCTNNNNNAGG	1 cut(s)	2075
BstF5I	GGATG	5 cut(s)	67, 150, 265, 1799, 2664
BstH2I	RGCGCY	1 cut(s)	2467
BstHHI	GCGC	3 cut(s)	1021, 2007, 2466
BstKTI	GATC	9 cut(s)	205, 960, 1051, 1189, 1913, 1927, 2286, 2600, 2710
BstMAI	GTCTC	3 cut(s)	484, 827, 1669
BstMBI	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
BstNI	CCWGG	2 cut(s)	819, 1905
BstNSI	RCATGY	1 cut(s)	2255
BstSCI	CCNGG	4 cut(s)	817, 1238, 1859, 1903
BstSFI	CTRYAG	1 cut(s)	2557
BstSLI	GKGCMC	1 cut(s)	1479
BstV2I	GAAGAC	5 cut(s)	272, 897, 993, 1208, 2556
BstX2I	RGATCY	3 cut(s)	1186, 1910, 2597
BstYI	RGATCY	3 cut(s)	1186, 1910, 2597
BstZ17I	GTATAC	1 cut(s)	325
BsuI	GTATCC	2 cut(s)	901, 2092
BsuRI	GGCC	9 cut(s)	237, 1308, 1543, 1802, 1944, 2501, 2564, 2618, 2669
BtgI	CCRYGG	2 cut(s)	497, 786
BtrI	CACGTC	1 cut(s)	157
BtsCI	GGATG	5 cut(s)	67, 150, 265, 1799, 2664
BtsIMutI	CAGTG	6 cut(s)	171, 987, 1143, 2080, 2237, 2521
Cac8I	GCNNGC	5 cut(s)	736, 1422, 1541, 1590, 2566
CaiI	CAGNNNCTG	2 cut(s)	2126, 2213
CciI	TCATGA	4 cut(s)	283, 598, 960, 1935
CfoI	GCGC	3 cut(s)	1021, 2007, 2466
Cfr13I	GGNCC	5 cut(s)	1801, 1942, 2500, 2562, 2668
CseI	GACGC	2 cut(s)	2321, 2342
CspCI	CAANNNNNGTGG	2 cut(s)	709, 744
DdeI	CTNAG	8 cut(s)	25, 215, 1071, 1446, 1954, 2064, 2072, 2345
DpnI	GATC	9 cut(s)	204, 959, 1050, 1188, 1912, 1926, 2285, 2599, 2709
DpnII	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
DraIII	CACNNNGTG	1 cut(s)	338
EaeI	YGGCCR	3 cut(s)	235, 1306, 1541
Eam1104I	CTCTTC	4 cut(s)	1560, 2076, 2470, 2577
EarI	CTCTTC	4 cut(s)	1560, 2076, 2470, 2577
EciI	GGCGGA	2 cut(s)	92, 1923
Eco130I	CCWWGG	1 cut(s)	885
Eco24I	GRGCYC	1 cut(s)	26
Eco31I	GGTCTC	1 cut(s)	1669
Eco57I	CTGAAG	8 cut(s)	208, 963, 1362, 2114, 2140, 2235, 2529, 2601
Eco72I	CACGTG	1 cut(s)	2222
EcoNI	CCTNNNNNAGG	1 cut(s)	2075
EcoRI	GAATTC	3 cut(s)	460, 853, 1400
EcoRII	CCWGG	2 cut(s)	817, 1903
EcoT14I	CCWWGG	1 cut(s)	885
EcoT38I	GRGCYC	1 cut(s)	26
ErhI	CCWWGG	1 cut(s)	885
FalI	AAGNNNNNCTT	4 cut(s)	1859, 1891, 2328, 2360
FauI	CCCGC	1 cut(s)	1048
FblI	GTMKAC	1 cut(s)	324
FokI	GGATG	5 cut(s)	74, 137, 272, 1806, 2671
FriOI	GRGCYC	1 cut(s)	26
FspAI	RTGCGCAY	1 cut(s)	2006
FspBI	CTAG	4 cut(s)	392, 471, 1724, 2022
FspI	TGCGCA	2 cut(s)	1020, 2006
GlaI	GCGC	3 cut(s)	1020, 2006, 2465
GsaI	CCCAGC	1 cut(s)	2507
HaeII	RGCGCY	1 cut(s)	2467
HaeIII	GGCC	9 cut(s)	237, 1308, 1543, 1802, 1944, 2501, 2564, 2618, 2669
HapII	CCGG	6 cut(s)	361, 446, 1239, 1860, 2531, 2569
HgaI	GACGC	2 cut(s)	2321, 2342
HhaI	GCGC	3 cut(s)	1021, 2007, 2466
Hin6I	GCGC	3 cut(s)	1019, 2005, 2464
HinP1I	GCGC	3 cut(s)	1019, 2005, 2464
HincII	GTYRAC	1 cut(s)	585
HindII	GTYRAC	1 cut(s)	585
HpaII	CCGG	6 cut(s)	361, 446, 1239, 1860, 2531, 2569
HphI	GGTGA	6 cut(s)	139, 350, 563, 587, 857, 1217
Hpy166II	GTNNAC	4 cut(s)	169, 325, 585, 1285
Hpy8I	GTNNAC	4 cut(s)	169, 325, 585, 1285
Hpy99I	CGWCG	1 cut(s)	2358
HpyCH4III	ACNGT	9 cut(s)	198, 271, 419, 589, 838, 901, 935, 1369, 2414
HpyCH4IV	ACGT	4 cut(s)	156, 1789, 2221, 2608
HpyF3I	CTNAG	8 cut(s)	25, 215, 1071, 1446, 1954, 2064, 2072, 2345
HpySE526I	ACGT	4 cut(s)	156, 1789, 2221, 2608
HspAI	GCGC	3 cut(s)	1019, 2005, 2464
Kpn2I	TCCGGA	2 cut(s)	360, 2530
Kzo9I	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
LguI	GCTCTTC	2 cut(s)	2076, 2577
LmnI	GCTCC	6 cut(s)	105, 499, 615, 722, 731, 1354
LweI	GCATC	5 cut(s)	201, 1675, 2119, 2319, 2704
MaeI	CTAG	4 cut(s)	392, 471, 1724, 2022
MaeII	ACGT	4 cut(s)	156, 1789, 2221, 2608
MaeIII	GTNAC	5 cut(s)	375, 448, 575, 649, 1879
MalI	GATC	9 cut(s)	204, 959, 1050, 1188, 1912, 1926, 2285, 2599, 2709
MbiI	CCGCTC	1 cut(s)	2628
MboI	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
MfeI	CAATTG	1 cut(s)	1560
MflI	RGATCY	3 cut(s)	1186, 1910, 2597
MhlI	GDGCHC	3 cut(s)	26, 1351, 1479
MlsI	TGGCCA	3 cut(s)	237, 1308, 1543
MluNI	TGGCCA	3 cut(s)	237, 1308, 1543
MlyI	GAGTC	2 cut(s)	397, 962
MmeI	TCCRAC	4 cut(s)	92, 806, 1626, 2413
Mox20I	TGGCCA	3 cut(s)	237, 1308, 1543
MroI	TCCGGA	2 cut(s)	360, 2530
MroXI	GAANNNNTTC	4 cut(s)	315, 801, 874, 1230
MscI	TGGCCA	3 cut(s)	237, 1308, 1543
MseI	TTAA	8 cut(s)	273, 591, 621, 773, 1083, 1455, 2172, 2493
MslI	CAYNNNNRTG	4 cut(s)	417, 455, 631, 1713
Msp20I	TGGCCA	3 cut(s)	237, 1308, 1543
MspA1I	CMGCKG	5 cut(s)	749, 1016, 1588, 2123, 2507
MspCI	CTTAAG	1 cut(s)	2492
MspI	CCGG	6 cut(s)	361, 446, 1239, 1860, 2531, 2569
MspR9I	CCNGG	4 cut(s)	819, 1240, 1861, 1905
MunI	CAATTG	1 cut(s)	1560
Mva1269I	GAATGC	3 cut(s)	801, 1489, 1931
MvaI	CCWGG	2 cut(s)	819, 1905
NciI	CCSGG	2 cut(s)	1240, 1861
NdeII	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
NlaIV	GGNNCC	8 cut(s)	101, 187, 611, 718, 733, 1003, 1912, 2502
NmuCI	GTSAC	3 cut(s)	448, 575, 1879
NsbI	TGCGCA	2 cut(s)	1020, 2006
NspI	RCATGY	1 cut(s)	2255
OliI	CACNNNNGTG	1 cut(s)	455
PagI	TCATGA	4 cut(s)	283, 598, 960, 1935
PciSI	GCTCTTC	2 cut(s)	2076, 2577
PcsI	WCGNNNNNNNCGW	1 cut(s)	1786
PctI	GAATGC	3 cut(s)	801, 1489, 1931
PdmI	GAANNNNTTC	4 cut(s)	315, 801, 874, 1230
PflMI	CCANNNNNTGG	2 cut(s)	572, 998
PleI	GAGTC	2 cut(s)	396, 962
PmaCI	CACGTG	1 cut(s)	2222
PmlI	CACGTG	1 cut(s)	2222
PpsI	GAGTC	2 cut(s)	396, 962
Ppu21I	YACGTR	2 cut(s)	2222, 2609
PshBI	ATTAAT	1 cut(s)	621
Psp6I	CCWGG	2 cut(s)	817, 1903
PspCI	CACGTG	1 cut(s)	2222
PspFI	CCCAGC	1 cut(s)	2503
PspGI	CCWGG	2 cut(s)	817, 1903
PspN4I	GGNNCC	8 cut(s)	101, 187, 611, 718, 733, 1003, 1912, 2502
PspPI	GGNCC	5 cut(s)	1801, 1942, 2500, 2562, 2668
PsrI	GAACNNNNNNTAC	2 cut(s)	387, 419
PstNI	CAGNNNCTG	2 cut(s)	2126, 2213
PsuI	RGATCY	3 cut(s)	1186, 1910, 2597
PvuII	CAGCTG	4 cut(s)	749, 1016, 2123, 2507
RseI	CAYNNNNRTG	4 cut(s)	417, 455, 631, 1713
SapI	GCTCTTC	2 cut(s)	2076, 2577
SaqAI	TTAA	8 cut(s)	273, 591, 621, 773, 1083, 1455, 2172, 2493
Sau3AI	GATC	9 cut(s)	202, 957, 1048, 1186, 1910, 1924, 2283, 2597, 2707
Sau96I	GGNCC	5 cut(s)	1801, 1942, 2500, 2562, 2668
ScaI	AGTACT	4 cut(s)	405, 996, 1329, 1848
SchI	GAGTC	2 cut(s)	397, 962
ScrFI	CCNGG	4 cut(s)	819, 1240, 1861, 1905
SduI	GDGCHC	3 cut(s)	26, 1351, 1479
SfaNI	GCATC	5 cut(s)	201, 1675, 2119, 2319, 2704
SfcI	CTRYAG	1 cut(s)	2557
SmiMI	CAYNNNNRTG	4 cut(s)	417, 455, 631, 1713
SmlI	CTYRAG	2 cut(s)	2441, 2492
SmoI	CTYRAG	2 cut(s)	2441, 2492
SsiI	CCGC	9 cut(s)	103, 1041, 1223, 1417, 1586, 1814, 1908, 2401, 2626
SspI	AATATT	1 cut(s)	1080
SspMI	CTAG	4 cut(s)	392, 471, 1724, 2022
StyD4I	CCNGG	4 cut(s)	817, 1238, 1859, 1903
StyI	CCWWGG	1 cut(s)	885
TaaI	ACNGT	9 cut(s)	198, 271, 419, 589, 838, 901, 935, 1369, 2414
TaiI	ACGT	4 cut(s)	159, 1792, 2224, 2611
TatI	WGTACW	6 cut(s)	193, 403, 994, 1327, 1408, 1846
TauI	GCSGC	2 cut(s)	1420, 1817
Tru1I	TTAA	8 cut(s)	273, 591, 621, 773, 1083, 1455, 2172, 2493
Tru9I	TTAA	8 cut(s)	273, 591, 621, 773, 1083, 1455, 2172, 2493
TscAI	CASTG	6 cut(s)	171, 987, 1150, 2080, 2244, 2521
TseFI	GTSAC	3 cut(s)	448, 575, 1879
Tsp45I	GTSAC	3 cut(s)	448, 575, 1879
TspGWI	ACGGA	3 cut(s)	486, 795, 803
TspRI	CASTG	6 cut(s)	171, 987, 1150, 2080, 2244, 2521
Van91I	CCANNNNNTGG	2 cut(s)	572, 998
Vha464I	CTTAAG	1 cut(s)	2492
VspI	ATTAAT	1 cut(s)	621
XagI	CCTNNNNNAGG	1 cut(s)	2075
XbaI	TCTAGA	2 cut(s)	391, 1723
XceI	RCATGY	1 cut(s)	2255
XcmI	CCANNNNNNNNNTGG	1 cut(s)	495
XmiI	GTMKAC	1 cut(s)	324
XmnI	GAANNNNTTC	4 cut(s)	315, 801, 874, 1230
XspI	CTAG	4 cut(s)	392, 471, 1724, 2022
ZrmI	AGTACT	4 cut(s)	405, 996, 1329, 1848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.