Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0006328

Physical Location & Seq

Genomic Coordinates Forward (+)

43235662 .. 43243564

Gene Structure 7903 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG259100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGGACAAACCGCTCGTGGGTTCTGAGATTCATGGATTTCATACAATGCAAGATTTGGATGTTGACACTATTATGGAGGAAGCCAAAGGACGATGGCTCCGCCCAAACGAAATTCATGCAATACTCTATAATTACAAGTATTTCACCATCCATGTCAAGCCAGTGAACTTGCCCCAAAGTGGCACCATTGTATTGTTTGACCGTAAGATGCTTAGAAACTTCAGGAAAGATGGTCATAACTGGAAAAAGAAAAAAGATGGGAAGACAGTCAAAGAAGCTCATGAACACTTGAAAGTTGGTAATGAAGAAAGGATTCATGTATACTATGCCCATGGCGAGGATAGCCCAACCTTTGTCCGGAGGTGTTATTGGCTGCTTGATAAGAGTCTAGAACACATAGTCCTTGTTCATTACCGTGAAACTCAAGAGTTGCAGGGTTCTCCAGCCACACCTGTGAATTCCAATTCAAATTCAAGTTCTGTCTCAGACCCATCTGCTCCTTGGCGTCTATCAGAAGAACTTGACTCCGGGGCTAACAATTCATATTATGGTGGTGCAAATGAACTTTTAGAGCCTGGTATTGGTTTGAATGTTAATAATCATGAACAAAGGCTCCATGATATCAATACACTTGAATGGGATGAGCTTTTGGTTACATATGATTCCAAAGGAGACAAAGTTTCAGGCTTTGACCAGCAGAATCAACTTGTGGGAAATGGCGCCATAAGTGGTGGAACCAGTGGTTTACCAGCTGAAGTTTCTTCTTTTGGCAATTTACTCGATCCAAGTGTGAGGACAGGAAACATTCATTTCAATCTTCCAGACAGTAATTATGTTCAGACACTGGAGGGTGAGGTGAATTCAAATGCACAGAGAATGGATTCTGTTGTCAAGGGCCCCTGTGATTCCTCGGACATTTTGGGTAATGATGGATTGCGTAGTCAGGACAGTTTTGGGAGGTGGATAAACCAGATTATGACCGACTCTCCGGGTTCAGTGGATGACCCAGTGCTTGAATCCTCATTTCCAGCTTCTCAGAGTTCATTTGCTTCTCCAGCAATGGAACATATTCAATCTTCTGTTCCTGAGCAAATATTTATCATAACCGATGTTTCACCTGCATGGGCTTTCTCAAATGAGAAAACAAAGATTCTGATCACTGGCTTCTTTCATCAAGAGTTTTTGGATCTTGCGAAGTCCAATCTACTTTGTATCTGTGGCGATGTATGTATTCCTGCTGAAATTGTTCAGGTTGGGGTATATCGATGTTTCATACCACCACATGTGCCCAGATGTGTGAACCTTTTCATTAGTCTTGATGGTCATAAACCCATCAGCCAAGTACAAAATTTTGAATATCGATCTCCTGTAATGAGTAACTTAGTAGTTCCTTCAGGAGAGAACAAATCGGAAGAGTTCCAATTACAGATGCGGCTTGCTCATTTGCTTTTCTCATCATCTAAAAGCTTGAACATTGCATCTAGTAAAGTATCACCACATACCCTCAAAGAAGCCAAGAAGTTTTCCCATAGAACTTCTCACATATCAAATAGCTGGCAATATTTGATCAAGTCAATTGAAGTCAACAATACTCCTTTACCACTGGCAAAGGATAGTTTGTTTGAACTTATTTTGAAGAACAGACTAAAAGATTGGCTGTTGGAGAAAGTTCTTGACAGCTCTAAAACCAAAGAATATGATGGTCATGGTCAAGGAGTAATCCACTTATGTGCTATTCTAGATTATACCTGGGCTGTTCGATTGTTTTCATGGTCTGGCTTGTCATTAGACTTTCGTGATAGACGTGGATGGACGGCCCTTCATTGGGCAGCATATCATGGAAGGGAGAAAATGGTTGCAGTTCTATTATCTGCAGGGGCAAAGCCAAACTTGGTCACAGATCCCACTTCAGAAAATCCTGGTGGTTGCACTGCTGCTGATCTTGCATTTATGAAGGGTTATGATGGCTTAGCAGCTTATCTTTCAGAAAAGGCTTTGCTAAGACAATTTGAGGACATGAGTATAGCTGGAAATGTTAGTGGTTCCCTCCAAACTAACACAAACTGTTATGAGCACTCTGAGAATCTCAGTGAGGAAGACCTGTATCTGAAGGATACTTTAACAGCCTATAGGACAGCTGCTGATGCAGCAGCACGAATACAAGCTGCCTTCAGGGAAAACGCACTAAAATTAAAGACTAAAGCAGTTGAATCTTACATTCCTGAGGATGAAGCACGCAGTATAATTGCAGCATTGAAGATTCAACATGCATTTCGCCACTATGATACACGAAAAAAGATGGCAGCTGCTGTCCGTATTCAATATAGGTTCCGTACATGGAAGATACGTCAAGAGTTCCTTAATATGCGCCGTCAAGCTGTCAAAATTCAAGCTGCTTTTCGAGCCTTCCAAACGCGGAAGCAGTACCGCAAAATTCTATGGGGAGTCGGAGTGCTTGAGAAAGCTGTTCTGCGGTGGCGCTTAAAGAGAAAAGGGCTTCGGGGGCTTCAGGTTGATCCCATTGAAGCTGATGGAGATGAGAAGCAGGAAAGTGATACAGAAGAGGACTTCTATCGAAACAGCCGAAAACAAGCTGAAGAGCGTGTTGAGAGATCCATTGTGCGGGTTCAGGCCATGTTCCGCTCCAAAAAAGCACAGCAAGAATATAGTAGGATGAAGTTGGCTCATACTGAAGCAAAGGTGAGTGATTTAGTTGGACCTTTTTTACTATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 CG-1 IQ TIG

Protein Analysis

911

Amino Acids

102.95

Weight (kDa)

7.02

Isoelectric Point (pI)

44.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	27 - 140	1.7e-46	CG-1 domain
Ank_2	PF12796	564 - 631	2.4e-07	Ankyrin repeats (3 copies)
Ank_2	PF12796	575 - 663	1.2e-07	Ankyrin repeats (3 copies)
Ank	PF00023	603 - 633	2.7e-07	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16940 AT3G16940 AT3G16940
fragaria_vesca	FvH4_4g19400 FvH4_4g19400
malus_domestica	MD16G1157900.v1.1
prunus_persica	Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1
pyrus_communis	pycom13g13450 pycom16g13290
rosa_chinensis	RchiOBHm_Chr4g0424431
rosa_laevigata	RLG00000007475
rosa_multiflora	Rmu_sc0003567.1_g000011
rosa_roxburghii	Rroxscaffold_5G00366370
rosa_rugosa	Rorug04G0197700 Rorug04G0197800
rosa_samantha	Rh4AG253800 Rh4BG259100 Rh4CG270400 Rh4DG253600
rosa_wichuraiana	Rw4G021920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1126
Acc36I	ACCTGC	1 cut(s)	1126
AccB1I	GGYRCC	2 cut(s)	182, 719
AccBSI	CCGCTC	2 cut(s)	13, 2643
AccI	GTMKAC	1 cut(s)	321
AccII	CGCG	1 cut(s)	2416
AccIII	TCCGGA	1 cut(s)	357
AciI	CCGC	8 cut(s)	11, 100, 1432, 2416, 2428, 2473, 2623, 2641
AclWI	GGATC	5 cut(s)	776, 1194, 1895, 2510, 2607
AcsI	RAATTY	7 cut(s)	111, 457, 469, 859, 1348, 2385, 2433
AcuI	CTGAAG	9 cut(s)	205, 774, 1377, 1893, 2129, 2155, 2492, 2616, 2712
AcyI	GRCGYC	2 cut(s)	505, 720
AfaI	GTAC	3 cut(s)	1344, 2335, 2426
AfiI	CCNNNNNNNGG	8 cut(s)	17, 179, 337, 357, 581, 1824, 1825, 2622
AflIII	ACRYGT	1 cut(s)	1282
AjiI	CACGTC	1 cut(s)	1805
AjnI	CCWGG	3 cut(s)	574, 1748, 1918
AleI	CACNNNNGTG	2 cut(s)	452, 1728
AloI	GAACNNNNNNTCC	4 cut(s)	384, 416, 597, 629
Alw21I	GWGCWC	1 cut(s)	2076
Alw26I	GTCTC	2 cut(s)	487, 666
AlwI	GGATC	5 cut(s)	776, 1194, 1895, 2510, 2607
AlwNI	CAGNNNCTG	3 cut(s)	844, 2141, 2309
Aor13HI	TCCGGA	1 cut(s)	357
AoxI	GGCC	3 cut(s)	895, 1815, 2631
ApaI	GGGCCC	1 cut(s)	899
ApoI	RAATTY	7 cut(s)	111, 457, 469, 859, 1348, 2385, 2433
Asp700I	GAANNNNTTC	5 cut(s)	312, 880, 1068, 1245, 1304
AspLEI	GCGC	3 cut(s)	722, 2370, 2481
AspS9I	GGNCC	4 cut(s)	895, 896, 1816, 2717
AsuC2I	CCSGG	2 cut(s)	529, 990
AsuHPI	GGTGA	6 cut(s)	136, 863, 868, 1107, 1485, 2713
AvaII	GGWCC	1 cut(s)	2717
AxyI	CCTNAGG	1 cut(s)	2223
BaeGI	GKGCMC	2 cut(s)	899, 1290
BanI	GGYRCC	2 cut(s)	182, 719
BanII	GRGCYC	1 cut(s)	899
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	2 cut(s)	269, 2103
Bbv12I	GWGCWC	1 cut(s)	2076
BceAI	ACGGC	2 cut(s)	1830, 2355
BcgI	CGANNNNNNTGC	4 cut(s)	98, 132, 760, 794
BciT130I	CCWGG	3 cut(s)	576, 1750, 1920
BciVI	GTATCC	1 cut(s)	2107
BclI	TGATCA	2 cut(s)	1155, 1566
BcnI	CCSGG	2 cut(s)	529, 990
BcoDI	GTCTC	2 cut(s)	487, 666
BfaI	CTAG	3 cut(s)	389, 1482, 1739
BfmI	CTRYAG	2 cut(s)	1872, 2128
BfoI	RGCGCY	2 cut(s)	723, 2482
BfuAI	ACCTGC	1 cut(s)	1126
BfuI	GTATCC	1 cut(s)	2107
BlpI	GCTNAGC	1 cut(s)	1969
Bme1390I	CCNGG	5 cut(s)	529, 576, 990, 1750, 1920
Bme18I	GGWCC	1 cut(s)	2717
BmgBI	CACGTC	1 cut(s)	1805
BmgT120I	GGNCC	4 cut(s)	895, 896, 1816, 2717
BmiI	GGNNCC	9 cut(s)	98, 184, 614, 721, 736, 897, 898, 2044, 2330
BmrFI	CCNGG	5 cut(s)	529, 576, 990, 1750, 1920
BmrI	ACTGGG	1 cut(s)	1001
BmsI	GCATC	4 cut(s)	198, 1419, 1487, 2134
BmuI	ACTGGG	1 cut(s)	1001
BpiI	GAAGAC	2 cut(s)	269, 2103
BpmI	CTGGAG	3 cut(s)	426, 866, 1038
Bpu10I	CCTNAGC	1 cut(s)	1086
Bpu1102I	GCTNAGC	1 cut(s)	1969
BpuEI	CTTGAG	2 cut(s)	408, 2477
BpuMI	CCSGG	2 cut(s)	529, 990
Bsa29I	ATCGAT	2 cut(s)	1264, 1360
BsaBI	GATNNNNATC	1 cut(s)	1154
BsaHI	GRCGYC	2 cut(s)	505, 720
BsaJI	CCNNGG	5 cut(s)	331, 500, 528, 909, 1749
BsaWI	WCCGGW	1 cut(s)	357
Bsc4I	CCNNNNNNNGG	8 cut(s)	17, 179, 337, 357, 581, 1824, 1825, 2622
Bse1I	ACTGG	7 cut(s)	161, 245, 738, 849, 1007, 1165, 1608
Bse21I	CCTNAGG	1 cut(s)	2223
Bse3DI	GCAATG	2 cut(s)	1065, 1473
Bse8I	GATNNNNATC	1 cut(s)	1154
BseAI	TCCGGA	1 cut(s)	357
BseBI	CCWGG	3 cut(s)	576, 1750, 1920
BseCI	ATCGAT	2 cut(s)	1264, 1360
BseDI	CCNNGG	5 cut(s)	331, 500, 528, 909, 1749
BseGI	GGATG	7 cut(s)	64, 147, 646, 1006, 1814, 2233, 2679
BseJI	GATNNNNATC	1 cut(s)	1154
BseLI	CCNNNNNNNGG	8 cut(s)	17, 179, 337, 357, 581, 1824, 1825, 2622
BseMI	GCAATG	2 cut(s)	1065, 1473
BseMII	CTCAG	7 cut(s)	15, 498, 1049, 1077, 2070, 2101, 2214
BseNI	ACTGG	7 cut(s)	161, 245, 738, 849, 1007, 1165, 1608
BseSI	GKGCMC	2 cut(s)	899, 1290
Bsh1236I	CGCG	1 cut(s)	2416
BshFI	GGCC	3 cut(s)	897, 1817, 2633
BshNI	GGYRCC	2 cut(s)	182, 719
BshVI	ATCGAT	2 cut(s)	1264, 1360
BsiHKAI	GWGCWC	1 cut(s)	2076
BsiSI	CCGG	3 cut(s)	358, 528, 989
BslI	CCNNNNNNNGG	8 cut(s)	17, 179, 337, 357, 581, 1824, 1825, 2622
BsmAI	GTCTC	2 cut(s)	487, 666
BsnI	GGCC	3 cut(s)	897, 1817, 2633
Bsp120I	GGGCCC	1 cut(s)	895
Bsp1286I	GDGCHC	3 cut(s)	899, 1290, 2076
Bsp13I	TCCGGA	1 cut(s)	357
Bsp143I	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
Bsp1720I	GCTNAGC	1 cut(s)	1969
Bsp19I	CCATGG	1 cut(s)	331
BspACI	CCGC	8 cut(s)	11, 100, 1432, 2416, 2428, 2473, 2623, 2641
BspANI	GGCC	3 cut(s)	897, 1817, 2633
BspCNI	CTCAG	7 cut(s)	16, 497, 1048, 1078, 2071, 2100, 2215
BspDI	ATCGAT	2 cut(s)	1264, 1360
BspEI	TCCGGA	1 cut(s)	357
BspFNI	CGCG	1 cut(s)	2416
BspHI	TCATGA	2 cut(s)	280, 601
BspLI	GGNNCC	9 cut(s)	98, 184, 614, 721, 736, 897, 898, 2044, 2330
BspMAI	CTGCAG	1 cut(s)	1876
BspMI	ACCTGC	1 cut(s)	1126
BspPI	GGATC	5 cut(s)	776, 1194, 1895, 2510, 2607
BspQI	GCTCTTC	1 cut(s)	2592
BspT107I	GGYRCC	2 cut(s)	182, 719
BsrBI	CCGCTC	2 cut(s)	13, 2643
BsrDI	GCAATG	2 cut(s)	1065, 1473
BsrI	ACTGG	7 cut(s)	161, 245, 738, 849, 1007, 1165, 1608
BssECI	CCNNGG	5 cut(s)	331, 500, 528, 909, 1749
BssMI	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
BssNAI	GTATAC	1 cut(s)	322
BssNI	GRCGYC	2 cut(s)	505, 720
BssSI	CACGAG	1 cut(s)	14
BssT1I	CCWWGG	2 cut(s)	331, 500
Bst1107I	GTATAC	1 cut(s)	322
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	3 cut(s)	576, 1750, 1920
Bst4CI	ACNGT	6 cut(s)	203, 268, 416, 827, 950, 2066
Bst6I	CTCTTC	3 cut(s)	1407, 2556, 2592
BstACI	GRCGYC	2 cut(s)	505, 720
BstC8I	GCNNGC	3 cut(s)	1437, 1556, 2236
BstDSI	CCRYGG	1 cut(s)	331
BstF5I	GGATG	7 cut(s)	64, 147, 646, 1006, 1814, 2233, 2679
BstFNI	CGCG	1 cut(s)	2416
BstH2I	RGCGCY	2 cut(s)	723, 2482
BstHHI	GCGC	3 cut(s)	722, 2370, 2481
BstKTI	GATC	9 cut(s)	784, 1158, 1189, 1364, 1569, 1903, 1942, 2518, 2615
BstMAI	GTCTC	2 cut(s)	487, 666
BstMBI	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
BstMWI	GCNNNNNNNGC	7 cut(s)	342, 1055, 1943, 2144, 2147, 2402, 2503
BstNI	CCWGG	3 cut(s)	576, 1750, 1920
BstNSI	RCATGY	2 cut(s)	1286, 2270
BstSCI	CCNGG	5 cut(s)	527, 574, 988, 1748, 1918
BstSFI	CTRYAG	2 cut(s)	1872, 2128
BstSLI	GKGCMC	2 cut(s)	899, 1290
BstUI	CGCG	1 cut(s)	2416
BstV2I	GAAGAC	2 cut(s)	269, 2103
BstX2I	RGATCY	3 cut(s)	1186, 1900, 2612
BstYI	RGATCY	3 cut(s)	1186, 1900, 2612
BstZ17I	GTATAC	1 cut(s)	322
Bsu15I	ATCGAT	2 cut(s)	1264, 1360
Bsu36I	CCTNAGG	1 cut(s)	2223
BsuI	GTATCC	1 cut(s)	2107
BsuRI	GGCC	3 cut(s)	897, 1817, 2633
BsuTUI	ATCGAT	2 cut(s)	1264, 1360
BtgI	CCRYGG	1 cut(s)	331
BtgZI	GCGATG	1 cut(s)	1236
BtrI	CACGTC	1 cut(s)	1805
BtsCI	GGATG	7 cut(s)	64, 147, 646, 1006, 1814, 2233, 2679
BtsI	GCAGTG	1 cut(s)	1929
BtsIMutI	CAGTG	9 cut(s)	168, 745, 842, 1002, 1014, 1158, 1601, 1929, 2095
BveI	ACCTGC	1 cut(s)	1126
Cac8I	GCNNGC	3 cut(s)	1437, 1556, 2236
CaiI	CAGNNNCTG	3 cut(s)	844, 2141, 2309
CciI	TCATGA	2 cut(s)	280, 601
CfoI	GCGC	3 cut(s)	722, 2370, 2481
Cfr13I	GGNCC	4 cut(s)	895, 896, 1816, 2717
ClaI	ATCGAT	2 cut(s)	1264, 1360
CseI	GACGC	1 cut(s)	494
Csp6I	GTAC	3 cut(s)	1343, 2334, 2425
CviQI	GTAC	3 cut(s)	1343, 2334, 2425
DinI	GGCGCC	1 cut(s)	721
DpnI	GATC	9 cut(s)	783, 1157, 1188, 1363, 1568, 1902, 1941, 2517, 2614
DpnII	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
Eam1104I	CTCTTC	3 cut(s)	1407, 2556, 2592
EarI	CTCTTC	3 cut(s)	1407, 2556, 2592
EciI	GGCGGA	1 cut(s)	89
Eco130I	CCWWGG	2 cut(s)	331, 500
Eco24I	GRGCYC	1 cut(s)	899
Eco32I	GATATC	1 cut(s)	622
Eco47I	GGWCC	1 cut(s)	2717
Eco57I	CTGAAG	9 cut(s)	205, 774, 1377, 1893, 2129, 2155, 2492, 2616, 2712
Eco81I	CCTNAGG	1 cut(s)	2223
EcoO109I	RGGNCCY	2 cut(s)	895, 896
EcoRI	GAATTC	2 cut(s)	457, 859
EcoRII	CCWGG	3 cut(s)	574, 1748, 1918
EcoRV	GATATC	1 cut(s)	622
EcoT14I	CCWWGG	2 cut(s)	331, 500
EcoT22I	ATGCAT	1 cut(s)	2272
EcoT38I	GRGCYC	1 cut(s)	899
EgeI	GGCGCC	1 cut(s)	721
EheI	GGCGCC	1 cut(s)	721
ErhI	CCWWGG	2 cut(s)	331, 500
FalI	AAGNNNNNCTT	4 cut(s)	1874, 1906, 2343, 2375
FauI	CCCGC	1 cut(s)	2616
FauNDI	CATATG	1 cut(s)	658
FbaI	TGATCA	2 cut(s)	1155, 1566
FblI	GTMKAC	1 cut(s)	321
FokI	GGATG	7 cut(s)	71, 134, 653, 1013, 1821, 2240, 2686
FriOI	GRGCYC	1 cut(s)	899
FspBI	CTAG	3 cut(s)	389, 1482, 1739
GlaI	GCGC	3 cut(s)	721, 2369, 2480
GsuI	CTGGAG	3 cut(s)	426, 866, 1038
HaeII	RGCGCY	2 cut(s)	723, 2482
HaeIII	GGCC	3 cut(s)	897, 1817, 2633
HapII	CCGG	3 cut(s)	358, 528, 989
HgaI	GACGC	1 cut(s)	494
HhaI	GCGC	3 cut(s)	722, 2370, 2481
Hin1I	GRCGYC	2 cut(s)	505, 720
Hin6I	GCGC	3 cut(s)	720, 2368, 2479
HinP1I	GCGC	3 cut(s)	720, 2368, 2479
HincII	GTYRAC	2 cut(s)	64, 1585
HindII	GTYRAC	2 cut(s)	64, 1585
HindIII	AAGCTT	1 cut(s)	1465
HpaII	CCGG	3 cut(s)	358, 528, 989
HphI	GGTGA	6 cut(s)	136, 863, 868, 1107, 1485, 2713
Hpy166II	GTNNAC	6 cut(s)	64, 166, 322, 746, 1300, 1585
Hpy8I	GTNNAC	6 cut(s)	64, 166, 322, 746, 1300, 1585
HpyAV	CCTTC	7 cut(s)	1401, 1829, 1836, 1948, 2104, 2179, 2416
HpyCH4III	ACNGT	6 cut(s)	203, 268, 416, 827, 950, 2066
HpyCH4IV	ACGT	2 cut(s)	1804, 2347
HpyF10VI	GCNNNNNNNGC	7 cut(s)	342, 1055, 1943, 2144, 2147, 2402, 2503
HpySE526I	ACGT	2 cut(s)	1804, 2347
Hsp92I	GRCGYC	2 cut(s)	505, 720
HspAI	GCGC	3 cut(s)	720, 2368, 2479
KasI	GGCGCC	1 cut(s)	719
Kpn2I	TCCGGA	1 cut(s)	357
Ksp22I	TGATCA	2 cut(s)	1155, 1566
Kzo9I	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
LguI	GCTCTTC	1 cut(s)	2592
LmnI	GCTCC	4 cut(s)	102, 502, 618, 2648
LweI	GCATC	4 cut(s)	198, 1419, 1487, 2134
MaeI	CTAG	3 cut(s)	389, 1482, 1739
MaeII	ACGT	2 cut(s)	1804, 2347
MaeIII	GTNAC	3 cut(s)	652, 1376, 1894
MalI	GATC	9 cut(s)	783, 1157, 1188, 1363, 1568, 1902, 1941, 2517, 2614
MbiI	CCGCTC	2 cut(s)	13, 2643
MboI	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
MfeI	CAATTG	1 cut(s)	1575
MflI	RGATCY	3 cut(s)	1186, 1900, 2612
MhlI	GDGCHC	3 cut(s)	899, 1290, 2076
Mly113I	GGCGCC	1 cut(s)	720
MlyI	GAGTC	4 cut(s)	394, 518, 977, 2454
MmeI	TCCRAC	3 cut(s)	1641, 2428, 2695
Mph1103I	ATGCAT	1 cut(s)	2272
MroI	TCCGGA	1 cut(s)	357
MroXI	GAANNNNTTC	5 cut(s)	312, 880, 1068, 1245, 1304
MseI	TTAA	5 cut(s)	594, 2120, 2192, 2361, 2483
MslI	CAYNNNNRTG	6 cut(s)	71, 414, 452, 634, 1120, 1728
MspA1I	CMGCKG	3 cut(s)	752, 2138, 2306
MspI	CCGG	3 cut(s)	358, 528, 989
MspR9I	CCNGG	5 cut(s)	529, 576, 990, 1750, 1920
MunI	CAATTG	1 cut(s)	1575
MvaI	CCWGG	3 cut(s)	576, 1750, 1920
MvnI	CGCG	1 cut(s)	2416
MwoI	GCNNNNNNNGC	7 cut(s)	342, 1055, 1943, 2144, 2147, 2402, 2503
NarI	GGCGCC	1 cut(s)	720
NciI	CCSGG	2 cut(s)	529, 990
NcoI	CCATGG	1 cut(s)	331
NdeI	CATATG	1 cut(s)	658
NdeII	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
NlaIV	GGNNCC	9 cut(s)	98, 184, 614, 721, 736, 897, 898, 2044, 2330
NmuCI	GTSAC	1 cut(s)	1894
NsiI	ATGCAT	1 cut(s)	2272
NspI	RCATGY	2 cut(s)	1286, 2270
OliI	CACNNNNGTG	2 cut(s)	452, 1728
PagI	TCATGA	2 cut(s)	280, 601
PaqCI	CACCTGC	1 cut(s)	1126
PciI	ACATGT	1 cut(s)	1282
PciSI	GCTCTTC	1 cut(s)	2592
PcsI	WCGNNNNNNNCGW	2 cut(s)	1801, 2581
PdmI	GAANNNNTTC	5 cut(s)	312, 880, 1068, 1245, 1304
PleI	GAGTC	4 cut(s)	393, 518, 977, 2453
PluTI	GGCGCC	1 cut(s)	723
PpsI	GAGTC	4 cut(s)	393, 518, 977, 2453
PscI	ACATGT	1 cut(s)	1282
Psp6I	CCWGG	3 cut(s)	574, 1748, 1918
PspGI	CCWGG	3 cut(s)	574, 1748, 1918
PspN4I	GGNNCC	9 cut(s)	98, 184, 614, 721, 736, 897, 898, 2044, 2330
PspOMI	GGGCCC	1 cut(s)	895
PspPI	GGNCC	4 cut(s)	895, 896, 1816, 2717
PstI	CTGCAG	1 cut(s)	1876
PstNI	CAGNNNCTG	3 cut(s)	844, 2141, 2309
PsuI	RGATCY	3 cut(s)	1186, 1900, 2612
PvuII	CAGCTG	3 cut(s)	752, 2138, 2306
RsaI	GTAC	3 cut(s)	1344, 2335, 2426
RsaNI	GTAC	3 cut(s)	1343, 2334, 2425
RseI	CAYNNNNRTG	6 cut(s)	71, 414, 452, 634, 1120, 1728
SapI	GCTCTTC	1 cut(s)	2592
SaqAI	TTAA	5 cut(s)	594, 2120, 2192, 2361, 2483
Sau3AI	GATC	9 cut(s)	781, 1155, 1186, 1361, 1566, 1900, 1939, 2515, 2612
Sau96I	GGNCC	4 cut(s)	895, 896, 1816, 2717
SchI	GAGTC	4 cut(s)	394, 518, 977, 2454
ScrFI	CCNGG	5 cut(s)	529, 576, 990, 1750, 1920
SduI	GDGCHC	3 cut(s)	899, 1290, 2076
SfaNI	GCATC	4 cut(s)	198, 1419, 1487, 2134
SfcI	CTRYAG	2 cut(s)	1872, 2128
SfoI	GGCGCC	1 cut(s)	721
SinI	GGWCC	1 cut(s)	2717
SmiMI	CAYNNNNRTG	6 cut(s)	71, 414, 452, 634, 1120, 1728
SmlI	CTYRAG	2 cut(s)	423, 2456
SmoI	CTYRAG	2 cut(s)	423, 2456
SsiI	CCGC	8 cut(s)	11, 100, 1432, 2416, 2428, 2473, 2623, 2641
SspDI	GGCGCC	1 cut(s)	719
SspI	AATATT	2 cut(s)	1095, 1562
SspMI	CTAG	3 cut(s)	389, 1482, 1739
StyD4I	CCNGG	5 cut(s)	527, 574, 988, 1748, 1918
StyI	CCWWGG	2 cut(s)	331, 500
TaaI	ACNGT	6 cut(s)	203, 268, 416, 827, 950, 2066
TaiI	ACGT	2 cut(s)	1807, 2350
TaqI	TCGA	6 cut(s)	780, 1264, 1360, 1759, 2401, 2575
TaqII	GACCGA	1 cut(s)	995
TatI	WGTACW	1 cut(s)	1342
TauI	GCSGC	1 cut(s)	1435
Tru1I	TTAA	5 cut(s)	594, 2120, 2192, 2361, 2483
Tru9I	TTAA	5 cut(s)	594, 2120, 2192, 2361, 2483
TscAI	CASTG	9 cut(s)	168, 745, 849, 1002, 1014, 1165, 1608, 1936, 2095
TseFI	GTSAC	1 cut(s)	1894
Tsp45I	GTSAC	1 cut(s)	1894
TspGWI	ACGGA	2 cut(s)	2303, 2321
TspRI	CASTG	9 cut(s)	168, 745, 849, 1002, 1014, 1165, 1608, 1936, 2095
VpaK11BI	GGWCC	1 cut(s)	2717
XapI	RAATTY	7 cut(s)	111, 457, 469, 859, 1348, 2385, 2433
XbaI	TCTAGA	2 cut(s)	388, 1738
XceI	RCATGY	2 cut(s)	1286, 2270
XcmI	CCANNNNNNNNNTGG	1 cut(s)	498
XmiI	GTMKAC	1 cut(s)	321
XmnI	GAANNNNTTC	5 cut(s)	312, 880, 1068, 1245, 1304
XspI	CTAG	3 cut(s)	389, 1482, 1739
Zsp2I	ATGCAT	1 cut(s)	2272

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.