Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0006328

Physical Location & Seq

Genomic Coordinates Forward (+)

48224330 .. 48232160

Gene Structure 7831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00366370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGGACAAACGGCTCGTGGGTTCTGAGATTCATGGGTTTCATACAATGCAAGATTTGGATGTTGACACTATTATGGAGGAAGCCAAAGGACGATGGCTCCGCCCAAACGAAATTCATGCAATACTCTATAATTACAAGTATTTCACCATCCATGTCAAGCCAGTGAACTTGCCCCAAAGTGGCACCATTGTATTGTTTGACCGTAAGATGCTTAGAAACTTCAGGAAAGATGGTCATAACTGGAAAAAGAAAAAGGATGGGAAGACAGTCAAAGAAGCTCATGAACACTTGAAAGTTGGTAATGAAGAAAGGATTCATGTATACTATGCCCATGGCGAGGATAGCCCAACCTTTGTCCGGAGGTGTTATTGGCTGCTTGATAAGAGTCTAGAACACATAGTCCTTGTTCATTACCGTGAAACACAAGAGTTGCAGGGTTCTCCAGCCACACCTGTGAATTCCAATTCAAATTCAAATTCTAGTTCTATCTCAGACCCATCTGCTCCTTGGCGTCTATCAGAAGAACTTGACTCTGGGGCTAACAATTCATATTATGGTGGTGCAAATGAACCTTTAGAGCCTGGTATTGGTTTGAATGTTAATAATCATGAACAAAGGCTCCATGATATCAATACACTTGAATGGGATGAGCTTTTGGTTACATATGATTCCAAAGGAGACAAAGTTTCAGGCTTTGACCAGCAGAATCAACTTGTGGGAAATGGCGCCGTAAGTGGTGGAACCAGTGGTTTACCAGCTGAAGTTTCTTCTTTTGGCAATTTACTCGATCCAAGTGTGAGGACAGGAAACATTCTTTTCAATCTTCCAGACAGTAATTATGTTCAGACACTGGAGGGTGAGGTGAATTCAAATGCACAGAGAATGGATTCTGTTGTCAAGGGCCCCTGTGATTCCTCGGACATTTTGGGTAATGATGGATTGCGTAGTCAGGACAGTTTTGGGAGGTGGATAAACCAGATTATGACCGACTCTCCGGGTTCAGCGGATGACCCAGTGCTTGAATCCTCATTTCCAGCTTCTCGGAGTTCATTTGCTTCTCCAGCAATGGAACATATTCAATCTTCTGTTCCTGAGCAAATATTTATCATAACCGATGTTTCACCTGCATGGGCTTTCTCAAATGAGAAAACAAAGATTCTGATCACTGGCTGCTTTCATCAAGAGTTTTTGGATCTTGCGAAGTCTAATCTACTTTGTATCTGTGGCGATGTATGTATTCCTGCTGAAATTGTTCAGGTTGGGGTATATCGATGTTTCATACCACCACATGTGCCCGGATTTGTGAACCTTTTCATTAGTCTTGATGGTCATAAACCCATCAGCCAAGTACAAAGTTTTGAATATCGATCTCCTGTAATGAGTAACTTAGTAGTTCCTTCAGGAGAGAACAAATCGGAAGAGTTCCAATTACAGATGCGGCTTGCTCATTTGCTTTTCTCATCATCTAAAAGCTTGAACATTGCATCTAGTAAAGTATCACCACATACCCTCAAAGAAGCCAAGAAGTTTGCCCATAGAACTTCTCACATATCAAATAGCTGGCAATATTTGATCAAGTCAATTGAAGTCAACAATACTCCTTTACCACTGGCAAAGGATAGTTTGTTTGAACTTATTTTGAAGAACAGACTTAAAGATTGGCTGTTGGAGAAAGTTCTTGACAGCTCTAAAACCAAAGAATATGATGGTCATGGTCAAGGAGTAATCCACTTATGTGCTATTCTAGATTATACCTGGGCTGTTCGATTGTTTTCATGGTCTGGCTTGTCATTAGACTTTCGTGATAGACGTGGATGGACGGCCCTTCATTGGGCGGCATATCATGGAAGGGAGAAAATGGTTGCAGTTCTATTATCTGCAGGGGCAAAGCCAAACTTGGTCACAGATCCCACTTCAGAAAACCCTGGTGGTTGCACTGCTGCTGATCTTGCATCCATGAAGGCTTATGAAGGCCTAGCAGCTTATCTTTCAGAAAAGGCTTTGTTAAGACAATTTGAGGACATGAGTATAGCTGGAAATGTTAGTGGTTCCCTCCAAACTAACACAAACTTTTATGAGCACTCTGAGAATCTCAGTGAGGAAGACCTGTATCTGAAGGATACTTTAACAGCCTATAGGACAGCTGCTGATGCAGCAGCACGAATACAAGCTGCCTTCAGGGAAAACGCACTAAAATTAAAGACTAAAGCAGTTGAATCTTACATTCCTGAGGATGAAGCACGCAGTATAATTGCAGCATTGAAGATTCAACATGCATTTCGGCACTATGATACACGAAAAAAGATGGCAGCTGCTGCCCGTATTCAATATAGGTTCCGTACATGGAAGATACGTCAAGAGTTCCTTAATATGCGCCGTCAAGCTGTCAAAATTCAAGCTGCTTTTCGAGCCTTCCAAACGCGGAAGCAGTACCGCAAAATTCTATGGGGAGTCGGAGTGCTTGAGAAAGCTGTTCTGCGTTGGCGCTTAAAGAGAAAAGGGCTTCGGGGGCTTCAGGTTGATCCCATTGAAGCTGATGGAGATGAGAAGCAGGAAAGTGATACTGAAGAGGACTTCTACCGAAACAGCCGAAAACAAGCTGAAGAGCGTGTTGAGAGATCCATTGTGCGGGTTCAGGCCATGTTCCGCTCCAAAAAAGCACAGCAAGAATATAGTAGGATGAAGTTGGCTCATACTGAAGCAAAGCTAGAATATGAAGATATTTTCGACCCTGACAATAACATGCGTGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 CG-1 IQ TIG

Protein Analysis

917

Amino Acids

103.69

Weight (kDa)

6.77

Isoelectric Point (pI)

45.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	27 - 140	1.7e-46	CG-1 domain
Ank_2	PF12796	571 - 633	1.2e-07	Ankyrin repeats (3 copies)
Ank	PF00023	605 - 635	2.7e-07	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16940 AT3G16940 AT3G16940
fragaria_vesca	FvH4_4g19400 FvH4_4g19400
malus_domestica	MD16G1157900.v1.1
prunus_persica	Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1
pyrus_communis	pycom13g13450 pycom16g13290
rosa_chinensis	RchiOBHm_Chr4g0424431
rosa_laevigata	RLG00000007475
rosa_multiflora	Rmu_sc0003567.1_g000011
rosa_roxburghii	Rroxscaffold_5G00366370
rosa_rugosa	Rorug04G0197700 Rorug04G0197800
rosa_samantha	Rh4AG253800 Rh4BG259100 Rh4CG270400 Rh4DG253600
rosa_wichuraiana	Rw4G021920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1132
Acc36I	ACCTGC	1 cut(s)	1132
AccB1I	GGYRCC	2 cut(s)	182, 725
AccBSI	CCGCTC	1 cut(s)	2649
AccI	GTMKAC	1 cut(s)	321
AccII	CGCG	1 cut(s)	2422
AccIII	TCCGGA	1 cut(s)	357
AciI	CCGC	8 cut(s)	100, 1004, 1438, 1835, 2422, 2434, 2629, 2647
AclWI	GGATC	5 cut(s)	782, 1200, 1901, 2516, 2613
AcsI	RAATTY	7 cut(s)	111, 457, 469, 475, 865, 2391, 2439
AcyI	GRCGYC	2 cut(s)	511, 726
AfaI	GTAC	3 cut(s)	1350, 2341, 2432
AfiI	CCNNNNNNNGG	7 cut(s)	179, 337, 357, 587, 1830, 1831, 2628
AflIII	ACRYGT	1 cut(s)	1288
AjiI	CACGTC	1 cut(s)	1811
AjnI	CCWGG	3 cut(s)	580, 1754, 1924
AleI	CACNNNNGTG	2 cut(s)	452, 1734
AloI	GAACNNNNNNTCC	4 cut(s)	384, 416, 603, 635
Alw21I	GWGCWC	1 cut(s)	2082
Alw26I	GTCTC	1 cut(s)	672
AlwI	GGATC	5 cut(s)	782, 1200, 1901, 2516, 2613
AlwNI	CAGNNNCTG	3 cut(s)	850, 2147, 2315
Aor13HI	TCCGGA	1 cut(s)	357
AoxI	GGCC	4 cut(s)	901, 1821, 1972, 2637
ApaI	GGGCCC	1 cut(s)	905
ApoI	RAATTY	7 cut(s)	111, 457, 469, 475, 865, 2391, 2439
Asp700I	GAANNNNTTC	5 cut(s)	312, 886, 1074, 1251, 1310
AspLEI	GCGC	3 cut(s)	728, 2376, 2487
AspS9I	GGNCC	3 cut(s)	901, 902, 1822
AsuC2I	CCSGG	2 cut(s)	996, 1296
AsuHPI	GGTGA	5 cut(s)	136, 869, 874, 1113, 1491
AxyI	CCTNAGG	1 cut(s)	2229
BaeGI	GKGCMC	2 cut(s)	905, 1296
BanI	GGYRCC	2 cut(s)	182, 725
BanII	GRGCYC	1 cut(s)	905
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	2 cut(s)	269, 2109
Bbv12I	GWGCWC	1 cut(s)	2082
BceAI	ACGGC	4 cut(s)	26, 713, 1836, 2361
BcgI	CGANNNNNNTGC	4 cut(s)	98, 132, 766, 800
BciT130I	CCWGG	3 cut(s)	582, 1756, 1926
BciVI	GTATCC	1 cut(s)	2113
BclI	TGATCA	2 cut(s)	1161, 1572
BcnI	CCSGG	2 cut(s)	996, 1296
BcoDI	GTCTC	1 cut(s)	672
BfaI	CTAG	6 cut(s)	389, 480, 1488, 1745, 1976, 2708
BfmI	CTRYAG	2 cut(s)	1878, 2134
BfoI	RGCGCY	2 cut(s)	729, 2488
BfuAI	ACCTGC	1 cut(s)	1132
BfuI	GTATCC	1 cut(s)	2113
Bme1390I	CCNGG	5 cut(s)	582, 996, 1296, 1756, 1926
BmgBI	CACGTC	1 cut(s)	1811
BmgT120I	GGNCC	3 cut(s)	901, 902, 1822
BmiI	GGNNCC	9 cut(s)	98, 184, 620, 727, 742, 903, 904, 2050, 2336
BmrFI	CCNGG	5 cut(s)	582, 996, 1296, 1756, 1926
BmrI	ACTGGG	1 cut(s)	1007
BmsI	GCATC	5 cut(s)	198, 1425, 1493, 1961, 2140
BmuI	ACTGGG	1 cut(s)	1007
BpiI	GAAGAC	2 cut(s)	269, 2109
BpmI	CTGGAG	3 cut(s)	426, 872, 1044
Bpu10I	CCTNAGC	1 cut(s)	1092
BpuEI	CTTGAG	1 cut(s)	2483
BpuMI	CCSGG	2 cut(s)	996, 1296
Bsa29I	ATCGAT	2 cut(s)	1270, 1366
BsaBI	GATNNNNATC	1 cut(s)	1160
BsaHI	GRCGYC	2 cut(s)	511, 726
BsaJI	CCNNGG	5 cut(s)	331, 506, 915, 1755, 1924
BsaWI	WCCGGW	1 cut(s)	357
Bsc4I	CCNNNNNNNGG	7 cut(s)	179, 337, 357, 587, 1830, 1831, 2628
Bse1I	ACTGG	7 cut(s)	161, 245, 744, 855, 1013, 1171, 1614
Bse21I	CCTNAGG	1 cut(s)	2229
Bse3DI	GCAATG	2 cut(s)	1071, 1479
Bse8I	GATNNNNATC	1 cut(s)	1160
BseAI	TCCGGA	1 cut(s)	357
BseBI	CCWGG	3 cut(s)	582, 1756, 1926
BseCI	ATCGAT	2 cut(s)	1270, 1366
BseDI	CCNNGG	5 cut(s)	331, 506, 915, 1755, 1924
BseGI	GGATG	9 cut(s)	64, 147, 262, 652, 1012, 1820, 1952, 2239, 2685
BseJI	GATNNNNATC	1 cut(s)	1160
BseLI	CCNNNNNNNGG	7 cut(s)	179, 337, 357, 587, 1830, 1831, 2628
BseMI	GCAATG	2 cut(s)	1071, 1479
BseMII	CTCAG	6 cut(s)	15, 504, 1083, 2076, 2107, 2220
BseNI	ACTGG	7 cut(s)	161, 245, 744, 855, 1013, 1171, 1614
BseSI	GKGCMC	2 cut(s)	905, 1296
Bsh1236I	CGCG	1 cut(s)	2422
BshFI	GGCC	4 cut(s)	903, 1823, 1974, 2639
BshNI	GGYRCC	2 cut(s)	182, 725
BshVI	ATCGAT	2 cut(s)	1270, 1366
BsiHKAI	GWGCWC	1 cut(s)	2082
BsiSI	CCGG	3 cut(s)	358, 995, 1296
BslI	CCNNNNNNNGG	7 cut(s)	179, 337, 357, 587, 1830, 1831, 2628
BsmAI	GTCTC	1 cut(s)	672
BsnI	GGCC	4 cut(s)	903, 1823, 1974, 2639
Bsp120I	GGGCCC	1 cut(s)	901
Bsp1286I	GDGCHC	3 cut(s)	905, 1296, 2082
Bsp13I	TCCGGA	1 cut(s)	357
Bsp143I	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
Bsp19I	CCATGG	1 cut(s)	331
BspACI	CCGC	8 cut(s)	100, 1004, 1438, 1835, 2422, 2434, 2629, 2647
BspANI	GGCC	4 cut(s)	903, 1823, 1974, 2639
BspCNI	CTCAG	6 cut(s)	16, 503, 1084, 2077, 2106, 2221
BspDI	ATCGAT	2 cut(s)	1270, 1366
BspEI	TCCGGA	1 cut(s)	357
BspFNI	CGCG	1 cut(s)	2422
BspHI	TCATGA	2 cut(s)	280, 607
BspLI	GGNNCC	9 cut(s)	98, 184, 620, 727, 742, 903, 904, 2050, 2336
BspMAI	CTGCAG	1 cut(s)	1882
BspMI	ACCTGC	1 cut(s)	1132
BspPI	GGATC	5 cut(s)	782, 1200, 1901, 2516, 2613
BspQI	GCTCTTC	1 cut(s)	2598
BspT107I	GGYRCC	2 cut(s)	182, 725
BsrBI	CCGCTC	1 cut(s)	2649
BsrDI	GCAATG	2 cut(s)	1071, 1479
BsrI	ACTGG	7 cut(s)	161, 245, 744, 855, 1013, 1171, 1614
BssECI	CCNNGG	5 cut(s)	331, 506, 915, 1755, 1924
BssMI	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
BssNAI	GTATAC	1 cut(s)	322
BssNI	GRCGYC	2 cut(s)	511, 726
BssSI	CACGAG	1 cut(s)	14
BssT1I	CCWWGG	2 cut(s)	331, 506
Bst1107I	GTATAC	1 cut(s)	322
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	3 cut(s)	582, 1756, 1926
Bst4CI	ACNGT	5 cut(s)	203, 268, 416, 833, 956
Bst6I	CTCTTC	3 cut(s)	1413, 2562, 2598
BstACI	GRCGYC	2 cut(s)	511, 726
BstAPI	GCANNNNNTGC	1 cut(s)	2315
BstC8I	GCNNGC	3 cut(s)	1443, 1562, 2242
BstDEI	CTNAG	8 cut(s)	24, 212, 490, 1092, 1387, 2085, 2093, 2229
BstDSI	CCRYGG	1 cut(s)	331
BstF5I	GGATG	9 cut(s)	64, 147, 262, 652, 1012, 1820, 1952, 2239, 2685
BstFNI	CGCG	1 cut(s)	2422
BstH2I	RGCGCY	2 cut(s)	729, 2488
BstHHI	GCGC	3 cut(s)	728, 2376, 2487
BstKTI	GATC	9 cut(s)	790, 1164, 1195, 1370, 1575, 1909, 1948, 2524, 2621
BstMAI	GTCTC	1 cut(s)	672
BstMBI	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
BstMWI	GCNNNNNNNGC	8 cut(s)	342, 1061, 1949, 2150, 2153, 2315, 2408, 2509
BstNI	CCWGG	3 cut(s)	582, 1756, 1926
BstNSI	RCATGY	3 cut(s)	1292, 2276, 2746
BstSCI	CCNGG	5 cut(s)	580, 994, 1294, 1754, 1924
BstSFI	CTRYAG	2 cut(s)	1878, 2134
BstSLI	GKGCMC	2 cut(s)	905, 1296
BstUI	CGCG	1 cut(s)	2422
BstV2I	GAAGAC	2 cut(s)	269, 2109
BstX2I	RGATCY	3 cut(s)	1192, 1906, 2618
BstYI	RGATCY	3 cut(s)	1192, 1906, 2618
BstZ17I	GTATAC	1 cut(s)	322
Bsu15I	ATCGAT	2 cut(s)	1270, 1366
Bsu36I	CCTNAGG	1 cut(s)	2229
BsuI	GTATCC	1 cut(s)	2113
BsuRI	GGCC	4 cut(s)	903, 1823, 1974, 2639
BsuTUI	ATCGAT	2 cut(s)	1270, 1366
BtgI	CCRYGG	1 cut(s)	331
BtgZI	GCGATG	1 cut(s)	1242
BtrI	CACGTC	1 cut(s)	1811
BtsCI	GGATG	9 cut(s)	64, 147, 262, 652, 1012, 1820, 1952, 2239, 2685
BtsI	GCAGTG	1 cut(s)	1935
BtsIMutI	CAGTG	8 cut(s)	168, 751, 848, 1020, 1164, 1607, 1935, 2101
BveI	ACCTGC	1 cut(s)	1132
Cac8I	GCNNGC	3 cut(s)	1443, 1562, 2242
CaiI	CAGNNNCTG	3 cut(s)	850, 2147, 2315
CciI	TCATGA	2 cut(s)	280, 607
CfoI	GCGC	3 cut(s)	728, 2376, 2487
Cfr13I	GGNCC	3 cut(s)	901, 902, 1822
ClaI	ATCGAT	2 cut(s)	1270, 1366
CseI	GACGC	1 cut(s)	500
Csp6I	GTAC	3 cut(s)	1349, 2340, 2431
CviQI	GTAC	3 cut(s)	1349, 2340, 2431
DdeI	CTNAG	8 cut(s)	24, 212, 490, 1092, 1387, 2085, 2093, 2229
DinI	GGCGCC	1 cut(s)	727
DpnI	GATC	9 cut(s)	789, 1163, 1194, 1369, 1574, 1908, 1947, 2523, 2620
DpnII	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
Eam1104I	CTCTTC	3 cut(s)	1413, 2562, 2598
EarI	CTCTTC	3 cut(s)	1413, 2562, 2598
EciI	GGCGGA	1 cut(s)	89
Eco130I	CCWWGG	2 cut(s)	331, 506
Eco147I	AGGCCT	1 cut(s)	1974
Eco24I	GRGCYC	1 cut(s)	905
Eco32I	GATATC	1 cut(s)	628
Eco81I	CCTNAGG	1 cut(s)	2229
EcoO109I	RGGNCCY	2 cut(s)	901, 902
EcoRI	GAATTC	2 cut(s)	457, 865
EcoRII	CCWGG	3 cut(s)	580, 1754, 1924
EcoRV	GATATC	1 cut(s)	628
EcoT14I	CCWWGG	2 cut(s)	331, 506
EcoT22I	ATGCAT	1 cut(s)	2278
EcoT38I	GRGCYC	1 cut(s)	905
EgeI	GGCGCC	1 cut(s)	727
EheI	GGCGCC	1 cut(s)	727
ErhI	CCWWGG	2 cut(s)	331, 506
FalI	AAGNNNNNCTT	4 cut(s)	1880, 1912, 2349, 2381
FauI	CCCGC	1 cut(s)	2622
FauNDI	CATATG	1 cut(s)	664
FbaI	TGATCA	2 cut(s)	1161, 1572
FblI	GTMKAC	1 cut(s)	321
FokI	GGATG	9 cut(s)	71, 134, 269, 659, 1019, 1827, 1939, 2246, 2692
FriOI	GRGCYC	1 cut(s)	905
FspBI	CTAG	6 cut(s)	389, 480, 1488, 1745, 1976, 2708
GlaI	GCGC	3 cut(s)	727, 2375, 2486
GsuI	CTGGAG	3 cut(s)	426, 872, 1044
HaeII	RGCGCY	2 cut(s)	729, 2488
HaeIII	GGCC	4 cut(s)	903, 1823, 1974, 2639
HapII	CCGG	3 cut(s)	358, 995, 1296
HgaI	GACGC	1 cut(s)	500
HhaI	GCGC	3 cut(s)	728, 2376, 2487
Hin1I	GRCGYC	2 cut(s)	511, 726
Hin6I	GCGC	3 cut(s)	726, 2374, 2485
HinP1I	GCGC	3 cut(s)	726, 2374, 2485
HincII	GTYRAC	2 cut(s)	64, 1591
HindII	GTYRAC	2 cut(s)	64, 1591
HindIII	AAGCTT	1 cut(s)	1471
HpaII	CCGG	3 cut(s)	358, 995, 1296
HphI	GGTGA	5 cut(s)	136, 869, 874, 1113, 1491
Hpy166II	GTNNAC	6 cut(s)	64, 166, 322, 752, 1306, 1591
Hpy8I	GTNNAC	6 cut(s)	64, 166, 322, 752, 1306, 1591
HpyAV	CCTTC	8 cut(s)	1407, 1835, 1842, 1954, 1964, 2110, 2185, 2422
HpyCH4III	ACNGT	5 cut(s)	203, 268, 416, 833, 956
HpyCH4IV	ACGT	2 cut(s)	1810, 2353
HpyF10VI	GCNNNNNNNGC	8 cut(s)	342, 1061, 1949, 2150, 2153, 2315, 2408, 2509
HpyF3I	CTNAG	8 cut(s)	24, 212, 490, 1092, 1387, 2085, 2093, 2229
HpySE526I	ACGT	2 cut(s)	1810, 2353
Hsp92I	GRCGYC	2 cut(s)	511, 726
HspAI	GCGC	3 cut(s)	726, 2374, 2485
KasI	GGCGCC	1 cut(s)	725
Kpn2I	TCCGGA	1 cut(s)	357
Ksp22I	TGATCA	2 cut(s)	1161, 1572
Kzo9I	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
LguI	GCTCTTC	1 cut(s)	2598
LmnI	GCTCC	4 cut(s)	102, 508, 624, 2654
LweI	GCATC	5 cut(s)	198, 1425, 1493, 1961, 2140
MaeI	CTAG	6 cut(s)	389, 480, 1488, 1745, 1976, 2708
MaeII	ACGT	2 cut(s)	1810, 2353
MaeIII	GTNAC	3 cut(s)	658, 1382, 1900
MalI	GATC	9 cut(s)	789, 1163, 1194, 1369, 1574, 1908, 1947, 2523, 2620
MbiI	CCGCTC	1 cut(s)	2649
MboI	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
MfeI	CAATTG	1 cut(s)	1581
MflI	RGATCY	3 cut(s)	1192, 1906, 2618
MhlI	GDGCHC	3 cut(s)	905, 1296, 2082
Mly113I	GGCGCC	1 cut(s)	726
MlyI	GAGTC	4 cut(s)	394, 524, 983, 2460
MmeI	TCCRAC	2 cut(s)	1647, 2434
Mph1103I	ATGCAT	1 cut(s)	2278
MroI	TCCGGA	1 cut(s)	357
MroXI	GAANNNNTTC	5 cut(s)	312, 886, 1074, 1251, 1310
MseI	TTAA	7 cut(s)	600, 1653, 2006, 2126, 2198, 2367, 2489
MslI	CAYNNNNRTG	6 cut(s)	71, 414, 452, 640, 1126, 1734
MspA1I	CMGCKG	4 cut(s)	758, 1004, 2144, 2312
MspI	CCGG	3 cut(s)	358, 995, 1296
MspR9I	CCNGG	5 cut(s)	582, 996, 1296, 1756, 1926
MunI	CAATTG	1 cut(s)	1581
MvaI	CCWGG	3 cut(s)	582, 1756, 1926
MvnI	CGCG	1 cut(s)	2422
MwoI	GCNNNNNNNGC	8 cut(s)	342, 1061, 1949, 2150, 2153, 2315, 2408, 2509
NarI	GGCGCC	1 cut(s)	726
NciI	CCSGG	2 cut(s)	996, 1296
NcoI	CCATGG	1 cut(s)	331
NdeI	CATATG	1 cut(s)	664
NdeII	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
NlaIV	GGNNCC	9 cut(s)	98, 184, 620, 727, 742, 903, 904, 2050, 2336
NmuCI	GTSAC	1 cut(s)	1900
NsiI	ATGCAT	1 cut(s)	2278
NspI	RCATGY	3 cut(s)	1292, 2276, 2746
OliI	CACNNNNGTG	2 cut(s)	452, 1734
PagI	TCATGA	2 cut(s)	280, 607
PaqCI	CACCTGC	1 cut(s)	1132
PceI	AGGCCT	1 cut(s)	1974
PciI	ACATGT	1 cut(s)	1288
PciSI	GCTCTTC	1 cut(s)	2598
PcsI	WCGNNNNNNNCGW	1 cut(s)	1807
PdmI	GAANNNNTTC	5 cut(s)	312, 886, 1074, 1251, 1310
PleI	GAGTC	4 cut(s)	393, 524, 983, 2459
PluTI	GGCGCC	1 cut(s)	729
PpsI	GAGTC	4 cut(s)	393, 524, 983, 2459
PscI	ACATGT	1 cut(s)	1288
Psp6I	CCWGG	3 cut(s)	580, 1754, 1924
PspGI	CCWGG	3 cut(s)	580, 1754, 1924
PspN4I	GGNNCC	9 cut(s)	98, 184, 620, 727, 742, 903, 904, 2050, 2336
PspOMI	GGGCCC	1 cut(s)	901
PspPI	GGNCC	3 cut(s)	901, 902, 1822
PstI	CTGCAG	1 cut(s)	1882
PstNI	CAGNNNCTG	3 cut(s)	850, 2147, 2315
PsuI	RGATCY	3 cut(s)	1192, 1906, 2618
PvuII	CAGCTG	3 cut(s)	758, 2144, 2312
RsaI	GTAC	3 cut(s)	1350, 2341, 2432
RsaNI	GTAC	3 cut(s)	1349, 2340, 2431
RseI	CAYNNNNRTG	6 cut(s)	71, 414, 452, 640, 1126, 1734
SapI	GCTCTTC	1 cut(s)	2598
SaqAI	TTAA	7 cut(s)	600, 1653, 2006, 2126, 2198, 2367, 2489
Sau3AI	GATC	9 cut(s)	787, 1161, 1192, 1367, 1572, 1906, 1945, 2521, 2618
Sau96I	GGNCC	3 cut(s)	901, 902, 1822
SchI	GAGTC	4 cut(s)	394, 524, 983, 2460
ScrFI	CCNGG	5 cut(s)	582, 996, 1296, 1756, 1926
SduI	GDGCHC	3 cut(s)	905, 1296, 2082
SfaNI	GCATC	5 cut(s)	198, 1425, 1493, 1961, 2140
SfcI	CTRYAG	2 cut(s)	1878, 2134
SfoI	GGCGCC	1 cut(s)	727
SmiMI	CAYNNNNRTG	6 cut(s)	71, 414, 452, 640, 1126, 1734
SmlI	CTYRAG	1 cut(s)	2462
SmoI	CTYRAG	1 cut(s)	2462
SseBI	AGGCCT	1 cut(s)	1974
SsiI	CCGC	8 cut(s)	100, 1004, 1438, 1835, 2422, 2434, 2629, 2647
SspDI	GGCGCC	1 cut(s)	725
SspI	AATATT	2 cut(s)	1101, 1568
SspMI	CTAG	6 cut(s)	389, 480, 1488, 1745, 1976, 2708
StuI	AGGCCT	1 cut(s)	1974
StyD4I	CCNGG	5 cut(s)	580, 994, 1294, 1754, 1924
StyI	CCWWGG	2 cut(s)	331, 506
TaaI	ACNGT	5 cut(s)	203, 268, 416, 833, 956
TaiI	ACGT	2 cut(s)	1813, 2356
TaqI	TCGA	6 cut(s)	786, 1270, 1366, 1765, 2407, 2727
TaqII	GACCGA	1 cut(s)	1001
TatI	WGTACW	1 cut(s)	1348
TauI	GCSGC	2 cut(s)	1441, 1838
Tru1I	TTAA	7 cut(s)	600, 1653, 2006, 2126, 2198, 2367, 2489
Tru9I	TTAA	7 cut(s)	600, 1653, 2006, 2126, 2198, 2367, 2489
TscAI	CASTG	8 cut(s)	168, 751, 855, 1020, 1171, 1614, 1942, 2101
TseFI	GTSAC	1 cut(s)	1900
Tsp45I	GTSAC	1 cut(s)	1900
TspGWI	ACGGA	1 cut(s)	2327
TspRI	CASTG	8 cut(s)	168, 751, 855, 1020, 1171, 1614, 1942, 2101
XapI	RAATTY	7 cut(s)	111, 457, 469, 475, 865, 2391, 2439
XbaI	TCTAGA	2 cut(s)	388, 1744
XceI	RCATGY	3 cut(s)	1292, 2276, 2746
XcmI	CCANNNNNNNNNTGG	1 cut(s)	504
XmiI	GTMKAC	1 cut(s)	321
XmnI	GAANNNNTTC	5 cut(s)	312, 886, 1074, 1251, 1310
XspI	CTAG	6 cut(s)	389, 480, 1488, 1745, 1976, 2708
Zsp2I	ATGCAT	1 cut(s)	2278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.