Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0006328

Physical Location & Seq

Genomic Coordinates Forward (+)

52490610 .. 52498380

Gene Structure 7771 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG270400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2862 bp

ATGGACAAACCGCTCGTGGGTTCTGAGATTCATGGATTTCATACAATGCAAGTGATTTTTTTTCAAGTGGTCAAATGGAAGCAAGAGCAAGGAAGATTGGATTTTGAAGTGCCTTCAGTGAAATTCGTTTGTATGGATGCTATAAAAACTAGTTGTGTAGATTTGGATGTTGACACTATTATGGAGGAAGCCAAAGGACGATGGCTCCGCCCAAACGAAATTCATGCAATACTCTATAATTACAAGTATTTCACCATCCATGTCAAGCCAGTGAACTTGCCCAAAAGTGGCACCATTGTATTGTTTGACCGTAAGATGCTTAGAAACTTCAGGAAAGATGGTCATAACTGGAAAAAGAAAAAAGATGGGAAGACAGTCAAAGAAGCTCATGAACACTTGAAAGTTGGTAATGAAGAAAGGATTCATGTATACTATGCCCATGGCGAGGATAGCTCAACCTTTGTCCGGAGGTGTTATTGGCTGCTTGATAAGAGTCTAGAACATATAGTCCTTGTTCATTACCGTGAAACACAAGAGTTGCAGGGTTCTCCAGCCACACCTGTGAATTCCAATTCCAATTCAAATTCTAGTTCTATCTCAGACCCATCTGCTCCTTGGCGTCTATCAGAAGAACTTGACTCCGGGGCTAACAATTCATATTATGGTGGTGCAAATGAACTTTTAGAGCCTGGTATCGGTTTGAATGTTAATAATCATGAACAAAGGCTCCATGATATCAATACACTTGAATGGGATGAGCTTTTGGTTACATATGATTCCAAAGGAGACAAAGTTTCAGGCTTTGACCAGCAGAATCAACTTGTGGGAAATGGCGCCATAAGTGGTGGAACCAGTGGTTTACCAGCTGAAGTTTCTTCTTTTGGCAATTTACTCGATCCAAGTGTGAGGACAGGAAACATTCATTTCAATCTTCCAGACAGTAATTATGTTCAGACACTGGAGGGTGAGGTGAATTCAAATGCACAGAGAATGGATTCTGTTGTCAAGGGCCCCTGTGATTCCTCAGACATTTTGGGTAATGATGGATTGCGTAGTCAGGACAGTTTTGGGAGGTGGATAAACCAGATTATGACCAACTCTCCGGGTTCAGTGGATGACCCAGTGCTTGAATCCTCATTTCCACCTTCTCAGAGTTCATTTGCTTCTCCAGCAATGGAACATATTCAATCTTCTGTTCCTGAGCAAATATTTATCATAACCGATGTTTCACCTGCATGGGCTTTCTCAAATGAGAAAACAAAGATTCTGATCACTGGCTTCTTTCATCAAGAGGTTTTGGATCTTGCGAAGTCCAATCTACTTTGTATCTGTGGCGATGTATGTATTCCTGCTGAAATTGTTCAGGTTGGGGTATATCGATGTTTCATACCACCACATGTGCCCAGATTTGTGAACCTTTTCATTAGTCTTGATGGTCAAAAACCCATCAGCCAAATACAAAATTTTGAATATCGATCTCCTGTAATGAGTAACTTAGTAGTTCCTTCAGGAGAGAACAAATTGGAAGAGTTCCAATTACAGATGCGGCTTGCTCATTTGCTTTTCTCATCATCTAAAAGCTTGAACATTGCATCTAGTAAAGTATCACCACATACCCTCAAAGAAGCCAAGAAGTTTTCCCATAGAACTTCTCACATATCAAATAGCTGGCAATATTTGATCAAGTCAATTGAAGTCAACAGTACTCCTTTACCACTGGCAAAGGATAGTTTGTTTGAACTTATTTTGAAGAACAGACTAAAAGATTGGCTGTTGGAGAAAGTTCTTGACAGCTCTAAAACCAAAGAATGTGATGGTCATGGTCAAGGAGTAATCCACTTATGTGCTATTCTAGATTATACCTGGGCTGTTCGATTGTTTTCATGGTCTGGCTTGTCATTAGACTTTCGTGATAGACGTGGATGGACGGCCCTTCATTGGGCGGCATATCATGGAAGGGAGAAAATGGTTGCAGTTCTATTATCTGCAGGGGCAAAGCCAAACTTGGTCACAGATCCCACTTCAGAAAACCCTGGTGGTTGCACTGCTGCTGATCTTGCATTCATGAAGGGTTATGAAGGCTTAGCAGCTTATCTTTCAGAAAAGGCTTTGCTAAGACAATTTGAGGACATGAGTATAGCTGGAAATGTTAGTGGTTCCCTCCAAACTAACACAAACTGTTATCAGCACTCTGAGAATCTCAGTGAGGAAGACCTGTATCTGAACGATACTTTAACAGCCTATAGGACAGCTGCTGATGCAGCAGCACGAATACAAGCTGCCTTCAGGGAAAACGCACTAAAATTAAAGACTAAAGCAGTTGAATCTTACAGTCCTGAGGATGAAGCACGCAGTATAATTGCAGCATTGAAGATTCAACATGCATTTCGCCACCATGATGCACGAAAAAAGATGGCAGCTGCTGCCCGTATTCAATATAGGTTCCGTACATGGAAGATACGTCAAGAGTTCCTTAATATGCGCCGTCAAGCTGTCAAAATTCAAGCTGCTTTTCGAGCCTTCCAAACGCGGAAGCAGTACCGCAAAATCCTATGGGGAGTCGGAGTGCTTGAGAAAGCTGTTCTGCGGTGGCGCTTAAAGAGAAAAGGGCTTCGGGGGCTTCAGGTTGATCCCATTGAAGCTGATGGAGATGAGAAGCAGGAAAGTGATACTGAAGAGGACTTCTACCGAAACAGCCGAAAACAAGCTGAAGAGCGTGTTGAGAGATCCATTGTGCGGGTTCAGGCCATGTTCCGCTCCAAAAAAGCACAGCAAGAATATAGTAGGATGAAGTTGGCTCATACTGAAGCAAAGCTGGAATATGAAGATATTTTCGACCCTGACAATAACATGCGTGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 CG-1 IQ TIG

Protein Analysis

953

Amino Acids

107.88

Weight (kDa)

7.02

Isoelectric Point (pI)

42.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	63 - 176	7.2e-46	CG-1 domain
Ank_2	PF12796	600 - 669	1.4e-07	Ankyrin repeats (3 copies)
Ank_2	PF12796	612 - 702	7.4e-08	Ankyrin repeats (3 copies)
Ank	PF00023	641 - 671	2.8e-07	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16940 AT3G16940 AT3G16940
fragaria_vesca	FvH4_4g19400 FvH4_4g19400
malus_domestica	MD16G1157900.v1.1
prunus_persica	Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1 Prupe.1G122800_v2.0.a1
pyrus_communis	pycom13g13450 pycom16g13290
rosa_chinensis	RchiOBHm_Chr4g0424431
rosa_laevigata	RLG00000007475
rosa_multiflora	Rmu_sc0003567.1_g000011
rosa_roxburghii	Rroxscaffold_5G00366370
rosa_rugosa	Rorug04G0197700 Rorug04G0197800
rosa_samantha	Rh4AG253800 Rh4BG259100 Rh4CG270400 Rh4DG253600
rosa_wichuraiana	Rw4G021920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1240
Acc36I	ACCTGC	1 cut(s)	1240
AccB1I	GGYRCC	2 cut(s)	290, 833
AccBSI	CCGCTC	2 cut(s)	13, 2757
AccI	GTMKAC	1 cut(s)	429
AccII	CGCG	1 cut(s)	2530
AccIII	TCCGGA	1 cut(s)	465
AciI	CCGC	9 cut(s)	11, 208, 1546, 1943, 2530, 2542, 2587, 2737, 2755
AclWI	GGATC	5 cut(s)	890, 1308, 2009, 2624, 2721
AcsI	RAATTY	7 cut(s)	122, 219, 565, 583, 973, 1462, 2499
AcyI	GRCGYC	2 cut(s)	619, 834
AfaI	GTAC	3 cut(s)	1705, 2449, 2540
AfiI	CCNNNNNNNGG	8 cut(s)	17, 287, 445, 465, 695, 1938, 1939, 2736
AflIII	ACRYGT	1 cut(s)	1396
AhlI	ACTAGT	1 cut(s)	149
AjiI	CACGTC	1 cut(s)	1919
AjnI	CCWGG	3 cut(s)	688, 1862, 2032
AleI	CACNNNNGTG	2 cut(s)	560, 1842
AloI	GAACNNNNNNTCC	4 cut(s)	492, 524, 711, 743
Alw26I	GTCTC	1 cut(s)	780
AlwI	GGATC	5 cut(s)	890, 1308, 2009, 2624, 2721
AlwNI	CAGNNNCTG	3 cut(s)	958, 2255, 2423
Aor13HI	TCCGGA	1 cut(s)	465
AoxI	GGCC	3 cut(s)	1009, 1929, 2745
ApaI	GGGCCC	1 cut(s)	1013
ApoI	RAATTY	7 cut(s)	122, 219, 565, 583, 973, 1462, 2499
Asp700I	GAANNNNTTC	5 cut(s)	420, 994, 1182, 1359, 1418
AspLEI	GCGC	3 cut(s)	836, 2484, 2595
AspS9I	GGNCC	3 cut(s)	1009, 1010, 1930
AsuC2I	CCSGG	2 cut(s)	643, 1104
AsuHPI	GGTGA	5 cut(s)	244, 977, 982, 1221, 1599
AxyI	CCTNAGG	1 cut(s)	2337
BaeGI	GKGCMC	2 cut(s)	1013, 1404
BanI	GGYRCC	2 cut(s)	290, 833
BanII	GRGCYC	1 cut(s)	1013
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	2 cut(s)	377, 2217
BceAI	ACGGC	2 cut(s)	1944, 2469
BcgI	CGANNNNNNTGC	4 cut(s)	206, 240, 874, 908
BciT130I	CCWGG	3 cut(s)	690, 1864, 2034
BclI	TGATCA	2 cut(s)	1269, 1680
BcnI	CCSGG	2 cut(s)	643, 1104
BcoDI	GTCTC	1 cut(s)	780
BcuI	ACTAGT	1 cut(s)	149
BfaI	CTAG	5 cut(s)	150, 497, 588, 1596, 1853
BfmI	CTRYAG	2 cut(s)	1986, 2242
BfoI	RGCGCY	2 cut(s)	837, 2596
BfuAI	ACCTGC	1 cut(s)	1240
BlpI	GCTNAGC	1 cut(s)	2083
BmcAI	AGTACT	1 cut(s)	1705
Bme1390I	CCNGG	5 cut(s)	643, 690, 1104, 1864, 2034
BmgBI	CACGTC	1 cut(s)	1919
BmgT120I	GGNCC	3 cut(s)	1009, 1010, 1930
BmiI	GGNNCC	9 cut(s)	206, 292, 728, 835, 850, 1011, 1012, 2158, 2444
BmrFI	CCNGG	5 cut(s)	643, 690, 1104, 1864, 2034
BmrI	ACTGGG	1 cut(s)	1115
BmsI	GCATC	6 cut(s)	127, 306, 1533, 1601, 2248, 2389
BmuI	ACTGGG	1 cut(s)	1115
BpiI	GAAGAC	2 cut(s)	377, 2217
BplI	GAGNNNNNCTC	2 cut(s)	437, 469
BpmI	CTGGAG	3 cut(s)	534, 980, 1152
Bpu10I	CCTNAGC	1 cut(s)	1200
Bpu1102I	GCTNAGC	1 cut(s)	2083
BpuEI	CTTGAG	1 cut(s)	2591
BpuMI	CCSGG	2 cut(s)	643, 1104
Bsa29I	ATCGAT	2 cut(s)	1378, 1474
BsaBI	GATNNNNATC	1 cut(s)	1268
BsaHI	GRCGYC	2 cut(s)	619, 834
BsaJI	CCNNGG	5 cut(s)	439, 614, 642, 1863, 2032
BsaWI	WCCGGW	1 cut(s)	465
Bsc4I	CCNNNNNNNGG	8 cut(s)	17, 287, 445, 465, 695, 1938, 1939, 2736
Bse1I	ACTGG	7 cut(s)	269, 353, 852, 963, 1121, 1279, 1722
Bse21I	CCTNAGG	1 cut(s)	2337
Bse3DI	GCAATG	2 cut(s)	1179, 1587
Bse8I	GATNNNNATC	1 cut(s)	1268
BseAI	TCCGGA	1 cut(s)	465
BseBI	CCWGG	3 cut(s)	690, 1864, 2034
BseCI	ATCGAT	2 cut(s)	1378, 1474
BseDI	CCNNGG	5 cut(s)	439, 614, 642, 1863, 2032
BseGI	GGATG	8 cut(s)	142, 172, 255, 760, 1120, 1928, 2347, 2793
BseJI	GATNNNNATC	1 cut(s)	1268
BseLI	CCNNNNNNNGG	8 cut(s)	17, 287, 445, 465, 695, 1938, 1939, 2736
BseMI	GCAATG	2 cut(s)	1179, 1587
BseMII	CTCAG	8 cut(s)	15, 612, 1038, 1163, 1191, 2184, 2215, 2328
BseNI	ACTGG	7 cut(s)	269, 353, 852, 963, 1121, 1279, 1722
BseSI	GKGCMC	2 cut(s)	1013, 1404
Bsh1236I	CGCG	1 cut(s)	2530
BshFI	GGCC	3 cut(s)	1011, 1931, 2747
BshNI	GGYRCC	2 cut(s)	290, 833
BshVI	ATCGAT	2 cut(s)	1378, 1474
BsiSI	CCGG	3 cut(s)	466, 642, 1103
BslI	CCNNNNNNNGG	8 cut(s)	17, 287, 445, 465, 695, 1938, 1939, 2736
BsmAI	GTCTC	1 cut(s)	780
BsmI	GAATGC	1 cut(s)	2060
BsnI	GGCC	3 cut(s)	1011, 1931, 2747
Bsp120I	GGGCCC	1 cut(s)	1009
Bsp1286I	GDGCHC	2 cut(s)	1013, 1404
Bsp13I	TCCGGA	1 cut(s)	465
Bsp143I	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
Bsp1720I	GCTNAGC	1 cut(s)	2083
Bsp19I	CCATGG	1 cut(s)	439
BspACI	CCGC	9 cut(s)	11, 208, 1546, 1943, 2530, 2542, 2587, 2737, 2755
BspANI	GGCC	3 cut(s)	1011, 1931, 2747
BspCNI	CTCAG	8 cut(s)	16, 611, 1037, 1162, 1192, 2185, 2214, 2329
BspDI	ATCGAT	2 cut(s)	1378, 1474
BspEI	TCCGGA	1 cut(s)	465
BspFNI	CGCG	1 cut(s)	2530
BspHI	TCATGA	3 cut(s)	388, 715, 2064
BspLI	GGNNCC	9 cut(s)	206, 292, 728, 835, 850, 1011, 1012, 2158, 2444
BspMAI	CTGCAG	1 cut(s)	1990
BspMI	ACCTGC	1 cut(s)	1240
BspPI	GGATC	5 cut(s)	890, 1308, 2009, 2624, 2721
BspQI	GCTCTTC	1 cut(s)	2706
BspT107I	GGYRCC	2 cut(s)	290, 833
BsrBI	CCGCTC	2 cut(s)	13, 2757
BsrDI	GCAATG	2 cut(s)	1179, 1587
BsrI	ACTGG	7 cut(s)	269, 353, 852, 963, 1121, 1279, 1722
BssECI	CCNNGG	5 cut(s)	439, 614, 642, 1863, 2032
BssMI	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
BssNAI	GTATAC	1 cut(s)	430
BssNI	GRCGYC	2 cut(s)	619, 834
BssSI	CACGAG	1 cut(s)	14
BssT1I	CCWWGG	2 cut(s)	439, 614
Bst1107I	GTATAC	1 cut(s)	430
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	3 cut(s)	690, 1864, 2034
Bst4CI	ACNGT	8 cut(s)	311, 376, 524, 941, 1064, 1703, 2180, 2333
Bst6I	CTCTTC	3 cut(s)	1521, 2670, 2706
BstACI	GRCGYC	2 cut(s)	619, 834
BstAPI	GCANNNNNTGC	1 cut(s)	2423
BstC8I	GCNNGC	3 cut(s)	1551, 1670, 2350
BstDSI	CCRYGG	1 cut(s)	439
BstF5I	GGATG	8 cut(s)	142, 172, 255, 760, 1120, 1928, 2347, 2793
BstFNI	CGCG	1 cut(s)	2530
BstH2I	RGCGCY	2 cut(s)	837, 2596
BstHHI	GCGC	3 cut(s)	836, 2484, 2595
BstKTI	GATC	9 cut(s)	898, 1272, 1303, 1478, 1683, 2017, 2056, 2632, 2729
BstMAI	GTCTC	1 cut(s)	780
BstMBI	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
BstMWI	GCNNNNNNNGC	8 cut(s)	450, 1169, 2057, 2258, 2261, 2423, 2516, 2617
BstNI	CCWGG	3 cut(s)	690, 1864, 2034
BstNSI	RCATGY	3 cut(s)	1400, 2384, 2854
BstSCI	CCNGG	5 cut(s)	641, 688, 1102, 1862, 2032
BstSFI	CTRYAG	2 cut(s)	1986, 2242
BstSLI	GKGCMC	2 cut(s)	1013, 1404
BstUI	CGCG	1 cut(s)	2530
BstV2I	GAAGAC	2 cut(s)	377, 2217
BstX2I	RGATCY	3 cut(s)	1300, 2014, 2726
BstYI	RGATCY	3 cut(s)	1300, 2014, 2726
BstZ17I	GTATAC	1 cut(s)	430
Bsu15I	ATCGAT	2 cut(s)	1378, 1474
Bsu36I	CCTNAGG	1 cut(s)	2337
BsuRI	GGCC	3 cut(s)	1011, 1931, 2747
BsuTUI	ATCGAT	2 cut(s)	1378, 1474
BtgI	CCRYGG	1 cut(s)	439
BtgZI	GCGATG	1 cut(s)	1350
BtrI	CACGTC	1 cut(s)	1919
BtsCI	GGATG	8 cut(s)	142, 172, 255, 760, 1120, 1928, 2347, 2793
BtsI	GCAGTG	1 cut(s)	2043
BveI	ACCTGC	1 cut(s)	1240
Cac8I	GCNNGC	3 cut(s)	1551, 1670, 2350
CaiI	CAGNNNCTG	3 cut(s)	958, 2255, 2423
CciI	TCATGA	3 cut(s)	388, 715, 2064
CfoI	GCGC	3 cut(s)	836, 2484, 2595
Cfr13I	GGNCC	3 cut(s)	1009, 1010, 1930
ClaI	ATCGAT	2 cut(s)	1378, 1474
CseI	GACGC	1 cut(s)	608
Csp6I	GTAC	3 cut(s)	1704, 2448, 2539
CviQI	GTAC	3 cut(s)	1704, 2448, 2539
DinI	GGCGCC	1 cut(s)	835
DpnI	GATC	9 cut(s)	897, 1271, 1302, 1477, 1682, 2016, 2055, 2631, 2728
DpnII	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
Eam1104I	CTCTTC	3 cut(s)	1521, 2670, 2706
EarI	CTCTTC	3 cut(s)	1521, 2670, 2706
EciI	GGCGGA	1 cut(s)	197
Eco130I	CCWWGG	2 cut(s)	439, 614
Eco24I	GRGCYC	1 cut(s)	1013
Eco32I	GATATC	1 cut(s)	736
Eco81I	CCTNAGG	1 cut(s)	2337
EcoO109I	RGGNCCY	2 cut(s)	1009, 1010
EcoRI	GAATTC	2 cut(s)	565, 973
EcoRII	CCWGG	3 cut(s)	688, 1862, 2032
EcoRV	GATATC	1 cut(s)	736
EcoT14I	CCWWGG	2 cut(s)	439, 614
EcoT22I	ATGCAT	1 cut(s)	2386
EcoT38I	GRGCYC	1 cut(s)	1013
EgeI	GGCGCC	1 cut(s)	835
EheI	GGCGCC	1 cut(s)	835
ErhI	CCWWGG	2 cut(s)	439, 614
FalI	AAGNNNNNCTT	4 cut(s)	1988, 2020, 2457, 2489
FauI	CCCGC	1 cut(s)	2730
FauNDI	CATATG	1 cut(s)	772
FbaI	TGATCA	2 cut(s)	1269, 1680
FblI	GTMKAC	1 cut(s)	429
FokI	GGATG	8 cut(s)	149, 179, 242, 767, 1127, 1935, 2354, 2800
FriOI	GRGCYC	1 cut(s)	1013
FspBI	CTAG	5 cut(s)	150, 497, 588, 1596, 1853
GlaI	GCGC	3 cut(s)	835, 2483, 2594
GsuI	CTGGAG	3 cut(s)	534, 980, 1152
HaeII	RGCGCY	2 cut(s)	837, 2596
HaeIII	GGCC	3 cut(s)	1011, 1931, 2747
HapII	CCGG	3 cut(s)	466, 642, 1103
HgaI	GACGC	1 cut(s)	608
HhaI	GCGC	3 cut(s)	836, 2484, 2595
Hin1I	GRCGYC	2 cut(s)	619, 834
Hin6I	GCGC	3 cut(s)	834, 2482, 2593
HinP1I	GCGC	3 cut(s)	834, 2482, 2593
HincII	GTYRAC	2 cut(s)	172, 1699
HindII	GTYRAC	2 cut(s)	172, 1699
HindIII	AAGCTT	1 cut(s)	1579
HpaII	CCGG	3 cut(s)	466, 642, 1103
HphI	GGTGA	5 cut(s)	244, 977, 982, 1221, 1599
Hpy166II	GTNNAC	6 cut(s)	172, 274, 430, 860, 1414, 1699
Hpy8I	GTNNAC	6 cut(s)	172, 274, 430, 860, 1414, 1699
HpyAV	CCTTC	9 cut(s)	123, 1155, 1515, 1943, 1950, 2062, 2072, 2293, 2530
HpyCH4III	ACNGT	8 cut(s)	311, 376, 524, 941, 1064, 1703, 2180, 2333
HpyCH4IV	ACGT	2 cut(s)	1918, 2461
HpyF10VI	GCNNNNNNNGC	8 cut(s)	450, 1169, 2057, 2258, 2261, 2423, 2516, 2617
HpySE526I	ACGT	2 cut(s)	1918, 2461
Hsp92I	GRCGYC	2 cut(s)	619, 834
HspAI	GCGC	3 cut(s)	834, 2482, 2593
KasI	GGCGCC	1 cut(s)	833
Kpn2I	TCCGGA	1 cut(s)	465
Ksp22I	TGATCA	2 cut(s)	1269, 1680
Kzo9I	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
LguI	GCTCTTC	1 cut(s)	2706
LmnI	GCTCC	4 cut(s)	210, 616, 732, 2762
LweI	GCATC	6 cut(s)	127, 306, 1533, 1601, 2248, 2389
MaeI	CTAG	5 cut(s)	150, 497, 588, 1596, 1853
MaeII	ACGT	2 cut(s)	1918, 2461
MaeIII	GTNAC	3 cut(s)	766, 1490, 2008
MalI	GATC	9 cut(s)	897, 1271, 1302, 1477, 1682, 2016, 2055, 2631, 2728
MbiI	CCGCTC	2 cut(s)	13, 2757
MboI	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
MfeI	CAATTG	1 cut(s)	1689
MflI	RGATCY	3 cut(s)	1300, 2014, 2726
MhlI	GDGCHC	2 cut(s)	1013, 1404
Mly113I	GGCGCC	1 cut(s)	834
MlyI	GAGTC	3 cut(s)	502, 632, 2568
MmeI	TCCRAC	2 cut(s)	1755, 2542
Mph1103I	ATGCAT	1 cut(s)	2386
MroI	TCCGGA	1 cut(s)	465
MroXI	GAANNNNTTC	5 cut(s)	420, 994, 1182, 1359, 1418
MseI	TTAA	5 cut(s)	708, 2234, 2306, 2475, 2597
MslI	CAYNNNNRTG	7 cut(s)	179, 522, 560, 748, 1234, 1842, 2397
MspA1I	CMGCKG	3 cut(s)	866, 2252, 2420
MspI	CCGG	3 cut(s)	466, 642, 1103
MspR9I	CCNGG	5 cut(s)	643, 690, 1104, 1864, 2034
MunI	CAATTG	1 cut(s)	1689
Mva1269I	GAATGC	1 cut(s)	2060
MvaI	CCWGG	3 cut(s)	690, 1864, 2034
MvnI	CGCG	1 cut(s)	2530
MwoI	GCNNNNNNNGC	8 cut(s)	450, 1169, 2057, 2258, 2261, 2423, 2516, 2617
NarI	GGCGCC	1 cut(s)	834
NciI	CCSGG	2 cut(s)	643, 1104
NcoI	CCATGG	1 cut(s)	439
NdeI	CATATG	1 cut(s)	772
NdeII	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
NlaIV	GGNNCC	9 cut(s)	206, 292, 728, 835, 850, 1011, 1012, 2158, 2444
NmuCI	GTSAC	1 cut(s)	2008
NsiI	ATGCAT	1 cut(s)	2386
NspI	RCATGY	3 cut(s)	1400, 2384, 2854
OliI	CACNNNNGTG	2 cut(s)	560, 1842
PagI	TCATGA	3 cut(s)	388, 715, 2064
PaqCI	CACCTGC	1 cut(s)	1240
PciI	ACATGT	1 cut(s)	1396
PciSI	GCTCTTC	1 cut(s)	2706
PcsI	WCGNNNNNNNCGW	1 cut(s)	1915
PctI	GAATGC	1 cut(s)	2060
PdmI	GAANNNNTTC	5 cut(s)	420, 994, 1182, 1359, 1418
PleI	GAGTC	3 cut(s)	501, 632, 2567
PluTI	GGCGCC	1 cut(s)	837
PpsI	GAGTC	3 cut(s)	501, 632, 2567
PscI	ACATGT	1 cut(s)	1396
Psp6I	CCWGG	3 cut(s)	688, 1862, 2032
PspGI	CCWGG	3 cut(s)	688, 1862, 2032
PspN4I	GGNNCC	9 cut(s)	206, 292, 728, 835, 850, 1011, 1012, 2158, 2444
PspOMI	GGGCCC	1 cut(s)	1009
PspPI	GGNCC	3 cut(s)	1009, 1010, 1930
PstI	CTGCAG	1 cut(s)	1990
PstNI	CAGNNNCTG	3 cut(s)	958, 2255, 2423
PsuI	RGATCY	3 cut(s)	1300, 2014, 2726
PvuII	CAGCTG	3 cut(s)	866, 2252, 2420
RsaI	GTAC	3 cut(s)	1705, 2449, 2540
RsaNI	GTAC	3 cut(s)	1704, 2448, 2539
RseI	CAYNNNNRTG	7 cut(s)	179, 522, 560, 748, 1234, 1842, 2397
SapI	GCTCTTC	1 cut(s)	2706
SaqAI	TTAA	5 cut(s)	708, 2234, 2306, 2475, 2597
Sau3AI	GATC	9 cut(s)	895, 1269, 1300, 1475, 1680, 2014, 2053, 2629, 2726
Sau96I	GGNCC	3 cut(s)	1009, 1010, 1930
ScaI	AGTACT	1 cut(s)	1705
SchI	GAGTC	3 cut(s)	502, 632, 2568
ScrFI	CCNGG	5 cut(s)	643, 690, 1104, 1864, 2034
SduI	GDGCHC	2 cut(s)	1013, 1404
SfaNI	GCATC	6 cut(s)	127, 306, 1533, 1601, 2248, 2389
SfcI	CTRYAG	2 cut(s)	1986, 2242
SfoI	GGCGCC	1 cut(s)	835
SmiMI	CAYNNNNRTG	7 cut(s)	179, 522, 560, 748, 1234, 1842, 2397
SmlI	CTYRAG	1 cut(s)	2570
SmoI	CTYRAG	1 cut(s)	2570
SpeI	ACTAGT	1 cut(s)	149
SsiI	CCGC	9 cut(s)	11, 208, 1546, 1943, 2530, 2542, 2587, 2737, 2755
SspDI	GGCGCC	1 cut(s)	833
SspI	AATATT	2 cut(s)	1209, 1676
SspMI	CTAG	5 cut(s)	150, 497, 588, 1596, 1853
StyD4I	CCNGG	5 cut(s)	641, 688, 1102, 1862, 2032
StyI	CCWWGG	2 cut(s)	439, 614
TaaI	ACNGT	8 cut(s)	311, 376, 524, 941, 1064, 1703, 2180, 2333
TaiI	ACGT	2 cut(s)	1921, 2464
TaqI	TCGA	6 cut(s)	894, 1378, 1474, 1873, 2515, 2835
TatI	WGTACW	1 cut(s)	1703
TauI	GCSGC	2 cut(s)	1549, 1946
Tru1I	TTAA	5 cut(s)	708, 2234, 2306, 2475, 2597
Tru9I	TTAA	5 cut(s)	708, 2234, 2306, 2475, 2597
TseFI	GTSAC	1 cut(s)	2008
Tsp45I	GTSAC	1 cut(s)	2008
TspGWI	ACGGA	1 cut(s)	2435
XapI	RAATTY	7 cut(s)	122, 219, 565, 583, 973, 1462, 2499
XbaI	TCTAGA	2 cut(s)	496, 1852
XceI	RCATGY	3 cut(s)	1400, 2384, 2854
XcmI	CCANNNNNNNNNTGG	1 cut(s)	612
XmiI	GTMKAC	1 cut(s)	429
XmnI	GAANNNNTTC	5 cut(s)	420, 994, 1182, 1359, 1418
XspI	CTAG	5 cut(s)	150, 497, 588, 1596, 1853
ZrmI	AGTACT	1 cut(s)	1705
Zsp2I	ATGCAT	1 cut(s)	2386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.