Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004581

Physical Location & Seq

Genomic Coordinates Reverse (-)

12747351 .. 12751963

Gene Structure 4613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1158900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGATGCAAGGAACAATGGGAGTTGAGAGAGAAGGTTCGAAAAGTGGAGCAGGTCATGTGGGTGGTTTCTTTCATTTATTTGACTGGACTGCAAAGTCTCGAAAAAAATTATTCTCTGGCAAGTCGGATTCATCAGAACGCTCAAAACAGGGAAAGAGAAGCCATGGGAACTTGCCAATGACGCGACAGCATCTGGTAGACGAGGATGAAACTGGGGCAGCGTCAAGCATCAGAGGTAGCAGCGATTATAGTTATGCTTCATCTGTTACAGATGAAGAAGGACTTGGAGCGAAAGCCCCTAGTGTGGTAGCCAAGCTTATGGGATTAGACTCCTTACCCACGTCCAGTTTTTCTGAGCCTTACTCTACCCCATATTTTGACACTCAATCTCTCCAAGATGCTCATTATCACAGGAGAAACGTCGAGTGCTATCATGACGATCAGTTAAGGTATTCTGGAAATCCCATAAAAAGGATGGATGGCCCTACCAGGAATTCTTTGGAGCCAAAGCCCCAGAATATGCAAAGCACGCCAATTGAGAGGTTCCAGACTGAAACATTGCCTCCTAGATCAGCTAAGTCTATTCCGCTCACACACCATAAACTTTTGTCTCCCATTAAGAATCCCGGCTTTGTTTCCACTAGGAATGCTGCTCACATAATGGAAGCTGCTGCAAAAATTATTGAACCTGGACCTCCAGCCACCGCCAAGGCCAAGATGCCTTTAGTAGGGGCCTCTTCTGTGCCCCTGAAGGTTCAGGCTCTCAAAGAAAAGGTGGAGGCATCCCGAAAAGTTCCACTGGTTGGGTCTTCTTCAGAAACCTTGAAAGATCAAGATTTAAAAGATAAAGTTGGAGCTGCTTACAAGAAAACGAAGCCTTCTGAGGTGTCCCGAAAGCCTGTTGAATCAAATGCTGCTAAGTCTCTTCAGGGACAGTCTTTGAATAAGAGCTGGAATGGATCTCTCGATTTGTCATTCAGGGCTTCTTCGGAGACAGAAGAAACTAAGGTGAAGTCCATTTCTCTTGCAATCCAAGCAAAGGTCAATGTTCAGAAAAGAGGTCAGAATTTAAGTAGAAATATAAGCTCAGTGGGTCAGAGAGAACAGAATGAGGTCATCTCAAACCAGCCCTTCCAGACCCAACCAAATGTTCAAAAGAATCTGCACAAGAAACCTTCTACACATAATGCTTCAGGTGCTCTTAAGCAGAATAACCAGAAACAAAATGGTGTAGTTGATAAAGAGAAATTACCTTCAAAGCCTTTGGTTTCCAACTCGCAGGGTAGAAAAGTGCTGTCTGGGGATTCTTCTTCTGGTCGTCACAAAAGCTCAGTTCGAAGTAGTGGAAACTCCAAAATTGGATCCAGGAAGTTAGGCTCAGAGGCAACGGATAGTGAAAAAGAAGTGTCCTACTCGAACGAAAGGAATTATCCTAGGAAGAAACGATCAATAGATGGAAACTTTCAGTTTAATAAGGATCATGCTGTTGATGATATGTTGAGTCACAAAAATCAGAAGCGAGTTCAATCTAATCCAGTTACTGACAGGCACTATAGTTGGGCTGAAGATTGCAGAAAGAAAGGCATGGATGTTGTTTCGTTTACATTCACAGCCCCACTTACACGATCACTGCTTGGCACTGACATCTCAGCTCTGGTTGCGCAGAAGAGCAACAGTCTTTGCACAGATCACCGAGGTAAACGGTTGTTGCTTGACACGGACAGTATGAAGCCATCCTCAGTGGGTTACAATGTGATAAGCGGTGATGCTTTGAGTGCACTTTTGGAACAAAAGTTAAGGGAATTATCTTATGGGAGTGAATCTTCTAGTCGTGAAGCTGCAAAAGTATGCTCAGCTTCAACTGCTTCAAATTTGGATGTGAAACCCAATTATAATGCAGTTAGCTCCATGCAAAGGTTAAATGACCAGAGAGACCAACATCTGTTGGTTACCAAGAAATCGGGTGATCGATTTGAAGCTGACACTCCTCCTGCATTCAGATTGAAACAGAAGTTTCAGGGAGTCGATAAGGCAGAGGAATATAGCAGCAGTCCTATTGAAGCTGGGCAGGTGCATTGCCGGCATCCTAGTCCAGTCTCTGTTCTGGAATCTTCTTTTTCAAATGAAAGTTATGGCTCTTCAATTACCACTGATAGTAACAGTACAGAAGCAAGCAGGTTGTGCTCATCCGTCCAAGCTTGGGAAGTTAATGTTTTCAGTTCTTCCAAGAAGTTCCATTCAGTAGAAGCTGACACCGAGTTATCAGATTCTGCTTCTTCAACATATACAGGAACTGTGGCAAGAACCCATGCAGCTACAGTTCACATAAAAGAATCTTTGAGATCAAATGAGTGGGAATTGGAATACATAAAGGAGACACTATGTAATGTCGAATTGATGTTTCAAGACTTTACATTGGGCCGGGCCAGGAAGATCATAAACCCTCATCTCTTCAATCTGCTGGAATGTCGAAGAGGACAGTCTGAAATTGATGGTGGAGAGTCCAGGGTAAGGCGAAAGGAACTATTTGATTGTACAAGTGAATGCTTGGACTTGAAGTGCAGGCGTTATGTGGGCGGGGGATACAGCGCATGGGTGAAAGGAGTAGCAATGGTGAGGAGAAAGGCGTCTTTAGCCGAAGAAGTGTACAAAGAACTCTCAGGTTGGAAAGGCATGTGGGAATGCATGGTGGACGAGCTCGTGGACAAGGACATGGGCAGCCCCTATGGCAGATGGCTGGACTTCGAAACCGATGCATTTGCACTAGGAGTAGATGTTGAGGGTCAAATATTTGATTCTTTGGTAGATGAAGTGGTTGCTGATATGTTGCAATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

946

Amino Acids

104.26

Weight (kDa)

9.0

Isoelectric Point (pI)

47.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VARLMGL	PF14383	87 - 118	3.2e-14	DUF761-associated sequence motif
DUF4378	PF14309	786 - 937	6.3e-34	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05750 AT3G05750 AT3G05750 AT3G05750 AT3G58650 AT5G26910 AT5G26910 AT5G26910 AT5G26910
fragaria_vesca	FvH4_4g19300 FvH4_4g19300 FvH4_4g19300
malus_domestica	MD13G1158100.v1.1 MD16G1158900.v1.1
prunus_persica	Prupe.1G125400_v2.0.a1 Prupe.1G125400_v2.0.a1 Prupe.1G125400_v2.0.a1
pyrus_communis	pycom13g13520 pycom16g13380
rosa_chinensis	RchiOBHm_Chr4g0424321
rosa_laevigata	RLG00000007486
rosa_multiflora	Rmu_sc0002260.1_g000005
rosa_roxburghii	Rroxscaffold_5G00366270
rosa_rugosa	Rorug04G0195900 Rorug04G0195900
rosa_samantha	Rh4AG252700 Rh4BG258100 Rh4CG269000 Rh4DG252400 Rh4DG252500
rosa_wichuraiana	Rw4G021790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1893
AarI	CACCTGC	1 cut(s)	2059
AasI	GACNNNNNNGTC	1 cut(s)	94
Acc16I	TGCGCA	1 cut(s)	1662
Acc36I	ACCTGC	3 cut(s)	41, 2059, 2166
AccB7I	CCANNNNNTGG	1 cut(s)	803
AccBSI	CCGCTC	1 cut(s)	589
AccI	GTMKAC	1 cut(s)	198
AccII	CGCG	1 cut(s)	184
AciI	CCGC	4 cut(s)	587, 705, 1761, 2577
AclWI	GGATC	4 cut(s)	967, 1356, 1369, 1485
AcsI	RAATTY	3 cut(s)	493, 1066, 1870
AcuI	CTGAAG	5 cut(s)	770, 798, 911, 1176, 1584
AcyI	GRCGYC	1 cut(s)	2627
AfaI	GTAC	3 cut(s)	2164, 2536, 2648
AfiI	CCNNNNNNNGG	8 cut(s)	304, 471, 728, 803, 1039, 1914, 2200, 2511
AflII	CTTAAG	1 cut(s)	1202
AjiI	CACGTC	1 cut(s)	342
AjnI	CCWGG	5 cut(s)	488, 688, 1364, 2426, 2504
AjuI	GAANNNNNNNTTGG	4 cut(s)	267, 299, 1134, 1166
Alw21I	GWGCWC	4 cut(s)	1201, 1780, 2186, 2700
Alw26I	GTCTC	7 cut(s)	102, 615, 927, 986, 1926, 2101, 2369
Alw44I	GTGCAC	1 cut(s)	1776
AlwI	GGATC	4 cut(s)	967, 1356, 1369, 1485
AlwNI	CAGNNNCTG	5 cut(s)	193, 1541, 2099, 2270, 2294
AoxI	GGCC	5 cut(s)	481, 711, 732, 2419, 2424
ApaLI	GTGCAC	1 cut(s)	1776
ApoI	RAATTY	3 cut(s)	493, 1066, 1870
AspA2I	CCTAGG	1 cut(s)	1433
AspLEI	GCGC	2 cut(s)	1663, 2591
AspS9I	GGNCC	5 cut(s)	482, 692, 732, 2419, 2424
AsuC2I	CCSGG	2 cut(s)	627, 2423
AsuHPI	GGTGA	6 cut(s)	1021, 1682, 1775, 1976, 2608, 2626
AsuII	TTCGAA	3 cut(s)	38, 1336, 2745
AvaII	GGWCC	1 cut(s)	692
AvrII	CCTAGG	1 cut(s)	1433
BaeGI	GKGCMC	2 cut(s)	747, 1780
BamHI	GGATCC	1 cut(s)	1361
BanII	GRGCYC	1 cut(s)	2700
BauI	CACGAG	1 cut(s)	2699
BbsI	GAAGAC	1 cut(s)	801
Bbv12I	GWGCWC	4 cut(s)	1201, 1780, 2186, 2700
BccI	CCATC	6 cut(s)	469, 473, 1448, 1741, 2486, 2727
BcgI	CGANNNNNNTGC	4 cut(s)	2735, 2741, 2769, 2775
BciT130I	CCWGG	5 cut(s)	490, 690, 1366, 2428, 2506
BciVI	GTATCC	1 cut(s)	2576
BcnI	CCSGG	2 cut(s)	627, 2423
BcoDI	GTCTC	7 cut(s)	102, 615, 927, 986, 1926, 2101, 2369
BfaI	CTAG	7 cut(s)	300, 567, 642, 1434, 1827, 2088, 2765
BfmI	CTRYAG	2 cut(s)	1552, 2316
BfrI	CTTAAG	1 cut(s)	1202
BfuAI	ACCTGC	3 cut(s)	41, 2059, 2166
BfuI	GTATCC	1 cut(s)	2576
BglI	GCCNNNNNGGC	1 cut(s)	2727
BlnI	CCTAGG	1 cut(s)	1433
BlpI	GCTNAGC	1 cut(s)	1852
Bme1390I	CCNGG	7 cut(s)	490, 627, 690, 1366, 2423, 2428, 2506
Bme18I	GGWCC	1 cut(s)	692
BmgBI	CACGTC	1 cut(s)	342
BmgT120I	GGNCC	5 cut(s)	482, 692, 732, 2419, 2424
BmiI	GGNNCC	4 cut(s)	504, 545, 733, 1363
BmrFI	CCNGG	7 cut(s)	490, 627, 690, 1366, 2423, 2428, 2506
BmrI	ACTGGG	1 cut(s)	222
BmsI	GCATC	8 cut(s)	199, 237, 388, 708, 791, 1756, 2092, 2743
BmuI	ACTGGG	1 cut(s)	222
BpiI	GAAGAC	1 cut(s)	801
BplI	GAGNNNNNCTC	4 cut(s)	347, 379, 1103, 1135
BpmI	CTGGAG	1 cut(s)	681
Bpu1102I	GCTNAGC	1 cut(s)	1852
Bpu14I	TTCGAA	3 cut(s)	38, 1336, 2745
BpuMI	CCSGG	2 cut(s)	627, 2423
Bsa29I	ATCGAT	1 cut(s)	1969
BsaHI	GRCGYC	1 cut(s)	2627
BsaI	GGTCTC	1 cut(s)	1926
BsaJI	CCNNGG	5 cut(s)	163, 708, 1433, 1693, 2505
BsaXI	ACNNNNNCTCC	4 cut(s)	547, 577, 2760, 2790
Bsc4I	CCNNNNNNNGG	8 cut(s)	304, 471, 728, 803, 1039, 1914, 2200, 2511
Bse118I	RCCGGY	1 cut(s)	2079
Bse1I	ACTGG	6 cut(s)	89, 217, 345, 804, 1535, 2093
Bse3DI	GCAATG	3 cut(s)	557, 2074, 2616
BseBI	CCWGG	5 cut(s)	490, 690, 1366, 2428, 2506
BseCI	ATCGAT	1 cut(s)	1969
BseDI	CCNNGG	5 cut(s)	163, 708, 1433, 1693, 2505
BseGI	GGATG	9 cut(s)	211, 480, 484, 782, 1594, 1733, 1882, 2083, 2186
BseLI	CCNNNNNNNGG	8 cut(s)	304, 471, 728, 803, 1039, 1914, 2200, 2511
BseMI	GCAATG	3 cut(s)	557, 2074, 2616
BseMII	CTCAG	9 cut(s)	345, 873, 1101, 1344, 1392, 1662, 1752, 1866, 2673
BseNI	ACTGG	6 cut(s)	89, 217, 345, 804, 1535, 2093
BseRI	GAGGAG	2 cut(s)	1977, 2632
BseSI	GKGCMC	2 cut(s)	747, 1780
BseYI	CCCAGC	1 cut(s)	2063
BsgI	GTGCAG	2 cut(s)	1148, 2581
Bsh1236I	CGCG	1 cut(s)	184
BshFI	GGCC	5 cut(s)	483, 713, 734, 2421, 2426
BshVI	ATCGAT	1 cut(s)	1969
BsiHKAI	GWGCWC	4 cut(s)	1201, 1780, 2186, 2700
BsiSI	CCGG	3 cut(s)	627, 2080, 2422
BslFI	GGGAC	2 cut(s)	874, 945
BslI	CCNNNNNNNGG	8 cut(s)	304, 471, 728, 803, 1039, 1914, 2200, 2511
BsmAI	GTCTC	7 cut(s)	102, 615, 927, 986, 1926, 2101, 2369
BsmFI	GGGAC	2 cut(s)	874, 945
BsmI	GAATGC	4 cut(s)	652, 1994, 2549, 2687
BsnI	GGCC	5 cut(s)	483, 713, 734, 2421, 2426
Bso31I	GGTCTC	1 cut(s)	1926
Bsp119I	TTCGAA	3 cut(s)	38, 1336, 2745
Bsp1286I	GDGCHC	5 cut(s)	747, 1201, 1780, 2186, 2700
Bsp1407I	TGTACA	2 cut(s)	2534, 2646
Bsp1720I	GCTNAGC	1 cut(s)	1852
Bsp19I	CCATGG	1 cut(s)	163
BspACI	CCGC	4 cut(s)	587, 705, 1761, 2577
BspANI	GGCC	5 cut(s)	483, 713, 734, 2421, 2426
BspCNI	CTCAG	9 cut(s)	346, 874, 1100, 1343, 1391, 1661, 1751, 1865, 2672
BspDI	ATCGAT	1 cut(s)	1969
BspFNI	CGCG	1 cut(s)	184
BspHI	TCATGA	1 cut(s)	433
BspLI	GGNNCC	4 cut(s)	504, 545, 733, 1363
BspMI	ACCTGC	3 cut(s)	41, 2059, 2166
BspPI	GGATC	4 cut(s)	967, 1356, 1369, 1485
BspQI	GCTCTTC	2 cut(s)	1661, 2143
BspT104I	TTCGAA	3 cut(s)	38, 1336, 2745
BspTI	CTTAAG	1 cut(s)	1202
BspTNI	GGTCTC	1 cut(s)	1926
BsrBI	CCGCTC	1 cut(s)	589
BsrDI	GCAATG	3 cut(s)	557, 2074, 2616
BsrFI	RCCGGY	1 cut(s)	2079
BsrGI	TGTACA	2 cut(s)	2534, 2646
BsrI	ACTGG	6 cut(s)	89, 217, 345, 804, 1535, 2093
BssAI	RCCGGY	1 cut(s)	2079
BssECI	CCNNGG	5 cut(s)	163, 708, 1433, 1693, 2505
BssNI	GRCGYC	1 cut(s)	2627
BssSI	CACGAG	1 cut(s)	2699
BssT1I	CCWWGG	3 cut(s)	163, 708, 1433
Bst2BI	CACGAG	1 cut(s)	2699
Bst2UI	CCWGG	5 cut(s)	490, 690, 1366, 2428, 2506
Bst4CI	ACNGT	8 cut(s)	936, 1676, 1704, 1724, 2162, 2296, 2320, 2481
Bst6I	CTCTTC	6 cut(s)	742, 930, 1661, 2143, 2456, 2467
BstACI	GRCGYC	1 cut(s)	2627
BstAFI	CTTAAG	1 cut(s)	1202
BstAPI	GCANNNNNTGC	1 cut(s)	2181
BstAUI	TGTACA	2 cut(s)	2534, 2646
BstBI	TTCGAA	3 cut(s)	38, 1336, 2745
BstC8I	GCNNGC	4 cut(s)	530, 2081, 2173, 2564
BstDSI	CCRYGG	1 cut(s)	163
BstEII	GGTNACC	1 cut(s)	1948
BstENI	CCTNNNNNAGG	1 cut(s)	726
BstF5I	GGATG	9 cut(s)	211, 480, 484, 782, 1594, 1733, 1882, 2083, 2186
BstFNI	CGCG	1 cut(s)	184
BstHHI	GCGC	2 cut(s)	1663, 2591
BstMAI	GTCTC	7 cut(s)	102, 615, 927, 986, 1926, 2101, 2369
BstNI	CCWGG	5 cut(s)	490, 690, 1366, 2428, 2506
BstNSI	RCATGY	1 cut(s)	2677
BstPI	GGTNACC	1 cut(s)	1948
BstSCI	CCNGG	7 cut(s)	488, 625, 688, 1364, 2421, 2426, 2504
BstSFI	CTRYAG	2 cut(s)	1552, 2316
BstSLI	GKGCMC	2 cut(s)	747, 1780
BstUI	CGCG	1 cut(s)	184
BstV2I	GAAGAC	1 cut(s)	801
BstX2I	RGATCY	2 cut(s)	959, 1361
BstXI	CCANNNNNNTGG	1 cut(s)	319
BstYI	RGATCY	2 cut(s)	959, 1361
Bsu15I	ATCGAT	1 cut(s)	1969
BsuI	GTATCC	1 cut(s)	2576
BsuRI	GGCC	5 cut(s)	483, 713, 734, 2421, 2426
BsuTUI	ATCGAT	1 cut(s)	1969
BtgI	CCRYGG	1 cut(s)	163
BtrI	CACGTC	1 cut(s)	342
BtsCI	GGATG	9 cut(s)	211, 480, 484, 782, 1594, 1733, 1882, 2083, 2186
BtsI	GCAGTG	1 cut(s)	1628
BtsIMutI	CAGTG	6 cut(s)	797, 1095, 1628, 1638, 1746, 2148
BveI	ACCTGC	3 cut(s)	41, 2059, 2166
Cac8I	GCNNGC	4 cut(s)	530, 2081, 2173, 2564
CaiI	CAGNNNCTG	5 cut(s)	193, 1541, 2099, 2270, 2294
CciI	TCATGA	1 cut(s)	433
CfoI	GCGC	2 cut(s)	1663, 2591
Cfr10I	RCCGGY	1 cut(s)	2079
Cfr13I	GGNCC	5 cut(s)	482, 692, 732, 2419, 2424
ClaI	ATCGAT	1 cut(s)	1969
CseI	GACGC	3 cut(s)	190, 210, 2616
Csp6I	GTAC	3 cut(s)	2163, 2535, 2647
CviQI	GTAC	3 cut(s)	2163, 2535, 2647
DraI	TTTAAA	1 cut(s)	840
DrdI	GACNNNNNNGTC	1 cut(s)	94
DseDI	GACNNNNNNGTC	1 cut(s)	94
Eam1104I	CTCTTC	6 cut(s)	742, 930, 1661, 2143, 2456, 2467
EarI	CTCTTC	6 cut(s)	742, 930, 1661, 2143, 2456, 2467
Ecl136II	GAGCTC	1 cut(s)	2698
Eco130I	CCWWGG	3 cut(s)	163, 708, 1433
Eco24I	GRGCYC	1 cut(s)	2700
Eco31I	GGTCTC	1 cut(s)	1926
Eco47I	GGWCC	1 cut(s)	692
Eco53kI	GAGCTC	1 cut(s)	2698
Eco57I	CTGAAG	5 cut(s)	770, 798, 911, 1176, 1584
Eco91I	GGTNACC	1 cut(s)	1948
EcoICRI	GAGCTC	1 cut(s)	2698
EcoNI	CCTNNNNNAGG	1 cut(s)	726
EcoO109I	RGGNCCY	1 cut(s)	732
EcoO65I	GGTNACC	1 cut(s)	1948
EcoRI	GAATTC	1 cut(s)	493
EcoRII	CCWGG	5 cut(s)	488, 688, 1364, 2426, 2504
EcoT14I	CCWWGG	3 cut(s)	163, 708, 1433
EcoT22I	ATGCAT	2 cut(s)	2687, 2758
EcoT38I	GRGCYC	1 cut(s)	2700
ErhI	CCWWGG	3 cut(s)	163, 708, 1433
FalI	AAGNNNNNCTT	2 cut(s)	267, 299
FaqI	GGGAC	2 cut(s)	874, 945
FauI	CCCGC	1 cut(s)	2570
FblI	GTMKAC	1 cut(s)	198
FokI	GGATG	9 cut(s)	218, 487, 491, 769, 1601, 1720, 1889, 2070, 2173
FriOI	GRGCYC	1 cut(s)	2700
FspBI	CTAG	7 cut(s)	300, 567, 642, 1434, 1827, 2088, 2765
FspI	TGCGCA	1 cut(s)	1662
GlaI	GCGC	2 cut(s)	1662, 2590
GsaI	CCCAGC	1 cut(s)	2067
GsuI	CTGGAG	1 cut(s)	681
HaeIII	GGCC	5 cut(s)	483, 713, 734, 2421, 2426
HapII	CCGG	3 cut(s)	627, 2080, 2422
HgaI	GACGC	3 cut(s)	190, 210, 2616
HhaI	GCGC	2 cut(s)	1663, 2591
Hin1I	GRCGYC	1 cut(s)	2627
Hin6I	GCGC	2 cut(s)	1661, 2589
HinP1I	GCGC	2 cut(s)	1661, 2589
HindIII	AAGCTT	2 cut(s)	314, 2196
HpaII	CCGG	3 cut(s)	627, 2080, 2422
HphI	GGTGA	6 cut(s)	1021, 1682, 1775, 1976, 2608, 2626
Hpy166II	GTNNAC	8 cut(s)	199, 1602, 1700, 1778, 2323, 2647, 2692, 2704
Hpy8I	GTNNAC	8 cut(s)	199, 1602, 1700, 1778, 2323, 2647, 2692, 2704
Hpy99I	CGWCG	1 cut(s)	425
HpyAV	CCTTC	7 cut(s)	26, 272, 745, 888, 1141, 1185, 1263
HpyCH4III	ACNGT	8 cut(s)	936, 1676, 1704, 1724, 2162, 2296, 2320, 2481
HpyCH4IV	ACGT	2 cut(s)	341, 420
HpySE526I	ACGT	2 cut(s)	341, 420
Hsp92I	GRCGYC	1 cut(s)	2627
HspAI	GCGC	2 cut(s)	1661, 2589
KroI	GCCGGC	1 cut(s)	2079
KroNI	GCCGGC	1 cut(s)	2081
LguI	GCTCTTC	2 cut(s)	1661, 2143
LmnI	GCTCC	5 cut(s)	47, 287, 502, 854, 1910
LweI	GCATC	8 cut(s)	199, 237, 388, 708, 791, 1756, 2092, 2743
MaeI	CTAG	7 cut(s)	300, 567, 642, 1434, 1827, 2088, 2765
MaeII	ACGT	2 cut(s)	341, 420
MaeIII	GTNAC	7 cut(s)	265, 1319, 1502, 1537, 1745, 1948, 2156
MbiI	CCGCTC	1 cut(s)	589
MfeI	CAATTG	2 cut(s)	534, 2831
MflI	RGATCY	2 cut(s)	959, 1361
MhlI	GDGCHC	5 cut(s)	747, 1201, 1780, 2186, 2700
MlyI	GAGTC	4 cut(s)	323, 1510, 2031, 2510
MmeI	TCCRAC	4 cut(s)	105, 832, 1296, 2645
Mph1103I	ATGCAT	2 cut(s)	2687, 2758
MroNI	GCCGGC	1 cut(s)	2079
MseI	TTAA	9 cut(s)	446, 618, 839, 1070, 1203, 1470, 1796, 1919, 2208
MslI	CAYNNNNRTG	1 cut(s)	60
MspCI	CTTAAG	1 cut(s)	1202
MspI	CCGG	3 cut(s)	627, 2080, 2422
MspR9I	CCNGG	7 cut(s)	490, 627, 690, 1366, 2423, 2428, 2506
MunI	CAATTG	2 cut(s)	534, 2831
Mva1269I	GAATGC	4 cut(s)	652, 1994, 2549, 2687
MvaI	CCWGG	5 cut(s)	490, 690, 1366, 2428, 2506
MvnI	CGCG	1 cut(s)	184
NaeI	GCCGGC	1 cut(s)	2081
NciI	CCSGG	2 cut(s)	627, 2423
NcoI	CCATGG	1 cut(s)	163
NgoMIV	GCCGGC	1 cut(s)	2079
NlaIV	GGNNCC	4 cut(s)	504, 545, 733, 1363
NmuCI	GTSAC	2 cut(s)	1319, 1502
NsbI	TGCGCA	1 cut(s)	1662
NsiI	ATGCAT	2 cut(s)	2687, 2758
NspI	RCATGY	1 cut(s)	2677
NspV	TTCGAA	3 cut(s)	38, 1336, 2745
PagI	TCATGA	1 cut(s)	433
PaqCI	CACCTGC	1 cut(s)	2059
PciSI	GCTCTTC	2 cut(s)	1661, 2143
PctI	GAATGC	4 cut(s)	652, 1994, 2549, 2687
PdiI	GCCGGC	1 cut(s)	2081
PflMI	CCANNNNNTGG	1 cut(s)	803
PfoI	TCCNGGA	1 cut(s)	1364
PleI	GAGTC	4 cut(s)	323, 1509, 2030, 2509
PpsI	GAGTC	4 cut(s)	323, 1509, 2030, 2509
PsiI	TTATAA	1 cut(s)	1893
Psp124BI	GAGCTC	1 cut(s)	2700
Psp6I	CCWGG	5 cut(s)	488, 688, 1364, 2426, 2504
PspEI	GGTNACC	1 cut(s)	1948
PspFI	CCCAGC	1 cut(s)	2063
PspGI	CCWGG	5 cut(s)	488, 688, 1364, 2426, 2504
PspN4I	GGNNCC	4 cut(s)	504, 545, 733, 1363
PspPI	GGNCC	5 cut(s)	482, 692, 732, 2419, 2424
PstNI	CAGNNNCTG	5 cut(s)	193, 1541, 2099, 2270, 2294
PsuI	RGATCY	2 cut(s)	959, 1361
RsaI	GTAC	3 cut(s)	2164, 2536, 2648
RsaNI	GTAC	3 cut(s)	2163, 2535, 2647
RseI	CAYNNNNRTG	1 cut(s)	60
SacI	GAGCTC	1 cut(s)	2700
SapI	GCTCTTC	2 cut(s)	1661, 2143
SaqAI	TTAA	9 cut(s)	446, 618, 839, 1070, 1203, 1470, 1796, 1919, 2208
Sau96I	GGNCC	5 cut(s)	482, 692, 732, 2419, 2424
SchI	GAGTC	4 cut(s)	323, 1510, 2031, 2510
ScrFI	CCNGG	7 cut(s)	490, 627, 690, 1366, 2423, 2428, 2506
SduI	GDGCHC	5 cut(s)	747, 1201, 1780, 2186, 2700
SfaNI	GCATC	8 cut(s)	199, 237, 388, 708, 791, 1756, 2092, 2743
SfcI	CTRYAG	2 cut(s)	1552, 2316
SfuI	TTCGAA	3 cut(s)	38, 1336, 2745
SinI	GGWCC	1 cut(s)	692
SmiMI	CAYNNNNRTG	1 cut(s)	60
SmlI	CTYRAG	1 cut(s)	1202
SmoI	CTYRAG	1 cut(s)	1202
SsiI	CCGC	4 cut(s)	587, 705, 1761, 2577
SspI	AATATT	1 cut(s)	2790
SspMI	CTAG	7 cut(s)	300, 567, 642, 1434, 1827, 2088, 2765
SstI	GAGCTC	1 cut(s)	2700
StyD4I	CCNGG	7 cut(s)	488, 625, 688, 1364, 2421, 2426, 2504
StyI	CCWWGG	3 cut(s)	163, 708, 1433
TaaI	ACNGT	8 cut(s)	936, 1676, 1704, 1724, 2162, 2296, 2320, 2481
TaiI	ACGT	2 cut(s)	344, 423
TatI	WGTACW	3 cut(s)	2162, 2534, 2646
Tru1I	TTAA	9 cut(s)	446, 618, 839, 1070, 1203, 1470, 1796, 1919, 2208
Tru9I	TTAA	9 cut(s)	446, 618, 839, 1070, 1203, 1470, 1796, 1919, 2208
TscAI	CASTG	6 cut(s)	804, 1095, 1635, 1645, 1746, 2155
TseFI	GTSAC	2 cut(s)	1319, 1502
Tsp45I	GTSAC	2 cut(s)	1319, 1502
TspDTI	ATGAA	8 cut(s)	62, 120, 222, 249, 288, 1742, 2139, 2823
TspGWI	ACGGA	3 cut(s)	1403, 1733, 2179
TspRI	CASTG	6 cut(s)	804, 1095, 1635, 1645, 1746, 2155
Van91I	CCANNNNNTGG	1 cut(s)	803
Vha464I	CTTAAG	1 cut(s)	1202
VneI	GTGCAC	1 cut(s)	1776
VpaK11BI	GGWCC	1 cut(s)	692
XagI	CCTNNNNNAGG	1 cut(s)	726
XapI	RAATTY	3 cut(s)	493, 1066, 1870
XceI	RCATGY	1 cut(s)	2677
XcmI	CCANNNNNNNNNTGG	3 cut(s)	472, 496, 1223
XmaJI	CCTAGG	1 cut(s)	1433
XmiI	GTMKAC	1 cut(s)	198
XspI	CTAG	7 cut(s)	300, 567, 642, 1434, 1827, 2088, 2765
Zsp2I	ATGCAT	2 cut(s)	2687, 2758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.