Rh4DG252400

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004581
Physical Location & Seq
Forward (+)
46771006 .. 46772990
1985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG252400.1

Sequence Viewer

Length: 1749 bp
ATGGGAGTTGAAAAAGAAGGTGTGAGAAATGGAGCAGGACATGTGGGGGGTTTCTTTCAGCTGTTTGACTGGTCTTCAAAATCTCGAAAAAAATTATTTTCGAGCAAGTCAGATTTACCAGAGCATTCAAAACTAGGAAAGAAAGGCTATCGAAACTTTCCAGTGACACGGAATCATCCGGTTGATGAGGATGAAACTGCGGTAACAACGAGTGTCAAAGGTGGCAGTGACTATAGTTGTGCTTCATCAGTTACAGATGAAGAAGGATATGGATCTAAAGCCCCTAGTGTAGTTGCCAGGCTAATGGGTTTGGACTCTTTACCCACATCCAATCTTTCTGAGCCCTACTCTACCCCATTTTTTGACACTCAGTCTTTCCAAGATGCTTCTTACCACAGGAAAAACGTTGATTATTATCATGATCATCAGTTAATGTTTTCTGGAAACCTTCTCATGGAGGGCCCTACTAGGAATCCTGTGGAGCCAAAGCCCCAGAAGGTGCATAGCAGACCTATTGAGAAGTTCCAAACTGAAATGTTACCTCCTAGATCAGCCAAGTCTATCCCAATTACACACCATAAGCTTTTGTCTCCTATTAAGAATCCTGGCTTTGTTCCCACTAAGACTGCTGCGCACATTATGGAAGCTGCTGCAAGAATGATTGAACCTGGACCGCAAGCTACTGTCAAGGCTAAGATGCCTCTAGTAGGGTCTTCTTCTGCACCTTTGAAGGTTCAGGCTCTCAAGGAAAAGGCAGAGGCTTCCCGGAAGGTGACACTGGTTGGGTCATCTTCAGAAACCTTCAAAGTCCGAGATTTAAAAGAGAAAGTGGAAGGTGCTTGTAAGACAGCTAGGCCTCCAGAAGTCTCTCGCAAGCCAGTTGAGTCAAATGCTGCTAAGTACCTTAAGGGTCAGTCTTTGAACAAGAGCTGGAATGGATCTGTAGATATGCCCTTCAGAGCTTCATCTGATACAGAAGAAGATACTTCAAAGAATAAAGGAAAGTCTATTTCTCTTGCAGTACAAGCAAAGGTCAATGTTCAGAAAAGAGGTCAGAATCCAAGTAAAAATAGAAGCTTGGTGGGGCAGAGAGAACAGAATGAGGCCAGCTCAAGCCAGCCCTTCAGGAGCCAACCAAATGTTCAAAAGAACTTGCATAGGAAACCTTCTACTCATAATGCTTCTGGTGCTCTTAAGCAGAATAATCAGAAACAAAATTGTCTAATGGATAAAGACAAATTACCTTCAAAACCTTTGGCTTCCAACTCGCAGGTCAGAAAAGCGCTTTCTGGGGATTCTTCTTCTGGGCGTTACAAAAGCTCTATTCGAAGTAATGGAAACTCCAAAGTTGTATCAAGGAAGTTAGGCTTAGAGGCAACAGATAGTGCAAAAGAAGTTTCCTATTCTAATACAACGAATTACACCCGGAAGAAGCGAACAATAGATGGAAATTTCCATTTTAATAAAGACCGGGTTGTTGATGGTATGTCGAGTGACAAAAATCAGAAGCCAGGTCAATCTAAATTAGTTACTGACAGACAGTATAGTTGGTCTGAAGATTGCAAAAAGAAAGGTGGCATGGATGTCGTTTCGTTTACATTCACTGCCCCTCTTACACGATCATTTCCTGGGACTGAAATCCCGGTTCAGGTCACACCGAAGAATAACAGTATTAGCATGGATCACCGGGGTAAAAGATTGTTGCTTGACGCTGATAGTATGAAGTGTCTTCATTGGGTTACAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

582

Amino Acids

64.17

Weight (kDa)

9.82

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 82 - 111 1.8e-16 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 633
Acc36I ACCTGC 1 cut(s) 1261
AciI CCGC 2 cut(s) 200, 674
AclI AACGTT 1 cut(s) 405
AclWI GGATC 3 cut(s) 280, 946, 1689
AcsI RAATTY 1 cut(s) 1450
AcuI CTGAAG 4 cut(s) 777, 940, 1108, 1575
AfaI GTAC 2 cut(s) 902, 1023
AfeI AGCGCT 1 cut(s) 1284
AfiI CCNNNNNNNGG 3 cut(s) 454, 707, 1648
AflII CTTAAG 2 cut(s) 905, 1193
AflIII ACRYGT 1 cut(s) 40
AhdI GACNNNNNGTC 1 cut(s) 370
AjnI CCWGG 5 cut(s) 296, 604, 667, 1510, 1627
AjuI GAANNNNNNNTTGG 2 cut(s) 1125, 1157
Alw21I GWGCWC 1 cut(s) 1192
Alw26I GTCTC 2 cut(s) 594, 871
AlwI GGATC 3 cut(s) 280, 946, 1689
Aor51HI AGCGCT 1 cut(s) 1284
AoxI GGCC 3 cut(s) 460, 854, 1104
ApaI GGGCCC 1 cut(s) 464
ApeKI GCWGC 4 cut(s) 629, 647, 650, 893
ApoI RAATTY 1 cut(s) 1450
AspLEI GCGC 2 cut(s) 634, 1285
AspS9I GGNCC 3 cut(s) 460, 461, 671
AsuC2I CCSGG 5 cut(s) 766, 1426, 1472, 1643, 1688
AsuHPI GGTGA 2 cut(s) 784, 1676
AsuII TTCGAA 1 cut(s) 1327
AvaII GGWCC 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 464
BanII GRGCYC 2 cut(s) 345, 464
BbsI GAAGAC 3 cut(s) 66, 705, 1721
Bbv12I GWGCWC 1 cut(s) 1192
BbvI GCAGC 4 cut(s) 616, 634, 637, 880
BccI CCATC 2 cut(s) 1439, 1475
BcgI CGANNNNNNTGC 2 cut(s) 1567, 1601
BciT130I CCWGG 5 cut(s) 298, 606, 669, 1512, 1629
BclI TGATCA 1 cut(s) 421
BcnI CCSGG 5 cut(s) 766, 1426, 1472, 1643, 1688
BcoDI GTCTC 2 cut(s) 594, 871
BfaI CTAG 6 cut(s) 134, 285, 468, 546, 704, 852
BfmI CTRYAG 2 cut(s) 232, 942
BfoI RGCGCY 1 cut(s) 1286
BfrI CTTAAG 2 cut(s) 905, 1193
BfuAI ACCTGC 1 cut(s) 1261
BisI GCNGC 4 cut(s) 630, 648, 651, 894
BlsI GCNGC 4 cut(s) 631, 649, 652, 895
Bme18I GGWCC 1 cut(s) 671
BmeRI GACNNNNNGTC 1 cut(s) 370
BmgT120I GGNCC 3 cut(s) 460, 461, 671
BmiI GGNNCC 3 cut(s) 462, 483, 1130
BmsI GCATC 2 cut(s) 373, 687
BpiI GAAGAC 3 cut(s) 66, 705, 1721
BplI GAGNNNNNCTC 4 cut(s) 332, 364, 1094, 1126
BpmI CTGGAG 1 cut(s) 843
Bpu14I TTCGAA 1 cut(s) 1327
BpuEI CTTGAG 2 cut(s) 728, 1096
BpuMI CCSGG 5 cut(s) 766, 1426, 1472, 1643, 1688
BsaBI GATNNNNATC 2 cut(s) 271, 414
BsaJI CCNNGG 2 cut(s) 1628, 1687
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 707, 1648
Bse1I ACTGG 4 cut(s) 74, 161, 783, 878
Bse8I GATNNNNATC 2 cut(s) 271, 414
BseBI CCWGG 5 cut(s) 298, 606, 669, 1512, 1629
BseDI CCNNGG 2 cut(s) 1628, 1687
BseGI GGATG 4 cut(s) 175, 196, 326, 1588
BseJI GATNNNNATC 2 cut(s) 271, 414
BseLI CCNNNNNNNGG 3 cut(s) 454, 707, 1648
BseMII CTCAG 2 cut(s) 330, 383
BseNI ACTGG 4 cut(s) 74, 161, 783, 878
BseSI GKGCMC 1 cut(s) 464
BseXI GCAGC 4 cut(s) 616, 634, 637, 880
BsgI GTGCAG 1 cut(s) 705
BshFI GGCC 3 cut(s) 462, 856, 1106
BsiHKAI GWGCWC 1 cut(s) 1192
BsiSI CCGG 6 cut(s) 179, 766, 1426, 1471, 1643, 1687
BslFI GGGAC 1 cut(s) 1645
BslI CCNNNNNNNGG 3 cut(s) 454, 707, 1648
BsmAI GTCTC 2 cut(s) 594, 871
BsmFI GGGAC 1 cut(s) 1645
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 3 cut(s) 462, 856, 1106
Bsp119I TTCGAA 1 cut(s) 1327
Bsp120I GGGCCC 1 cut(s) 460
Bsp1286I GDGCHC 3 cut(s) 345, 464, 1192
Bsp143I GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
BspACI CCGC 2 cut(s) 200, 674
BspANI GGCC 3 cut(s) 462, 856, 1106
BspCNI CTCAG 2 cut(s) 331, 382
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 3 cut(s) 462, 483, 1130
BspMI ACCTGC 1 cut(s) 1261
BspPI GGATC 3 cut(s) 280, 946, 1689
BspT104I TTCGAA 1 cut(s) 1327
BspTI CTTAAG 2 cut(s) 905, 1193
BsrI ACTGG 4 cut(s) 74, 161, 783, 878
BssECI CCNNGG 2 cut(s) 1628, 1687
BssMI GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
Bst2UI CCWGG 5 cut(s) 298, 606, 669, 1512, 1629
Bst4CI ACNGT 3 cut(s) 685, 1542, 1670
BstAFI CTTAAG 2 cut(s) 905, 1193
BstBI TTCGAA 1 cut(s) 1327
BstC8I GCNNGC 4 cut(s) 678, 875, 1108, 1118
BstDEI CTNAG 6 cut(s) 339, 369, 621, 693, 897, 1369
BstENI CCTNNNNNAGG 1 cut(s) 705
BstF5I GGATG 4 cut(s) 175, 196, 326, 1588
BstH2I RGCGCY 1 cut(s) 1286
BstHHI GCGC 2 cut(s) 634, 1285
BstKTI GATC 6 cut(s) 275, 424, 551, 941, 1622, 1684
BstMAI GTCTC 2 cut(s) 594, 871
BstMBI GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
BstMWI GCNNNNNNNGC 2 cut(s) 1025, 1187
BstNI CCWGG 5 cut(s) 298, 606, 669, 1512, 1629
BstNSI RCATGY 1 cut(s) 44
BstSFI CTRYAG 2 cut(s) 232, 942
BstSLI GKGCMC 1 cut(s) 464
BstV1I GCAGC 4 cut(s) 616, 634, 637, 880
BstV2I GAAGAC 3 cut(s) 66, 705, 1721
BstX2I RGATCY 2 cut(s) 272, 938
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 2 cut(s) 272, 938
BsuRI GGCC 3 cut(s) 462, 856, 1106
BtsCI GGATG 4 cut(s) 175, 196, 326, 1588
BtsI GCAGTG 2 cut(s) 232, 1602
BtsIMutI CAGTG 4 cut(s) 168, 232, 776, 1602
BveI ACCTGC 1 cut(s) 1261
Cac8I GCNNGC 4 cut(s) 678, 875, 1108, 1118
CciI TCATGA 1 cut(s) 418
CfoI GCGC 2 cut(s) 634, 1285
Cfr13I GGNCC 3 cut(s) 460, 461, 671
CseI GACGC 1 cut(s) 1718
Csp6I GTAC 2 cut(s) 901, 1022
CviAII CATG 5 cut(s) 41, 419, 454, 1579, 1678
CviQI GTAC 2 cut(s) 901, 1022
DdeI CTNAG 6 cut(s) 339, 369, 621, 693, 897, 1369
DpnI GATC 6 cut(s) 274, 423, 550, 940, 1621, 1683
DpnII GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
DraI TTTAAA 1 cut(s) 819
DriI GACNNNNNGTC 1 cut(s) 370
Eam1105I GACNNNNNGTC 1 cut(s) 370
Eco147I AGGCCT 1 cut(s) 856
Eco24I GRGCYC 2 cut(s) 345, 464
Eco47I GGWCC 1 cut(s) 671
Eco47III AGCGCT 1 cut(s) 1284
Eco57I CTGAAG 4 cut(s) 777, 940, 1108, 1575
EcoNI CCTNNNNNAGG 1 cut(s) 705
EcoO109I RGGNCCY 2 cut(s) 460, 461
EcoRII CCWGG 5 cut(s) 296, 604, 667, 1510, 1627
EcoT38I GRGCYC 2 cut(s) 345, 464
FaeI CATG 5 cut(s) 44, 422, 457, 1582, 1681
FalI AAGNNNNNCTT 2 cut(s) 1352, 1384
FaqI GGGAC 1 cut(s) 1645
FatI CATG 5 cut(s) 40, 418, 453, 1578, 1677
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 4 cut(s) 630, 648, 651, 894
FokI GGATG 4 cut(s) 162, 203, 313, 1595
FriOI GRGCYC 2 cut(s) 345, 464
Fsp4HI GCNGC 4 cut(s) 630, 648, 651, 894
FspBI CTAG 6 cut(s) 134, 285, 468, 546, 704, 852
FspI TGCGCA 1 cut(s) 633
GlaI GCGC 2 cut(s) 633, 1284
GluI GCNGC 4 cut(s) 630, 648, 651, 894
GsuI CTGGAG 1 cut(s) 843
HaeII RGCGCY 1 cut(s) 1286
HaeIII GGCC 3 cut(s) 462, 856, 1106
HapII CCGG 6 cut(s) 179, 766, 1426, 1471, 1643, 1687
HgaI GACGC 1 cut(s) 1718
HhaI GCGC 2 cut(s) 634, 1285
Hin1II CATG 5 cut(s) 44, 422, 457, 1582, 1681
Hin6I GCGC 2 cut(s) 632, 1283
HinP1I GCGC 2 cut(s) 632, 1283
HindIII AAGCTT 2 cut(s) 581, 1075
HinfI GANTC 7 cut(s) 172, 314, 472, 601, 884, 1057, 1295
HpaII CCGG 6 cut(s) 179, 766, 1426, 1471, 1643, 1687
HphI GGTGA 2 cut(s) 784, 1676
Hpy166II GTNNAC 1 cut(s) 1596
Hpy188III TCNNGA 5 cut(s) 84, 419, 441, 860, 1126
Hpy8I GTNNAC 1 cut(s) 1596
HpyCH4III ACNGT 3 cut(s) 685, 1542, 1670
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 7 cut(s) 502, 653, 722, 1019, 1156, 1388, 1563
HpyF10VI GCNNNNNNNGC 2 cut(s) 1025, 1187
HpyF3I CTNAG 6 cut(s) 339, 369, 621, 693, 897, 1369
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 5 cut(s) 44, 422, 457, 1582, 1681
HspAI GCGC 2 cut(s) 632, 1283
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
LmnI GCTCC 3 cut(s) 32, 481, 1128
Lsp1109I GCAGC 4 cut(s) 616, 634, 637, 880
LweI GCATC 2 cut(s) 373, 687
MaeI CTAG 6 cut(s) 134, 285, 468, 546, 704, 852
MaeII ACGT 1 cut(s) 405
MalI GATC 6 cut(s) 274, 423, 550, 940, 1621, 1683
MboI GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
MflI RGATCY 2 cut(s) 272, 938
MhlI GDGCHC 3 cut(s) 345, 464, 1192
MluCI AATT 7 cut(s) 92, 567, 1216, 1238, 1417, 1450, 1523
MlyI GAGTC 2 cut(s) 308, 893
MmeI TCCRAC 1 cut(s) 1287
MseI TTAA 6 cut(s) 431, 597, 818, 906, 1194, 1461
MspA1I CMGCKG 1 cut(s) 61
MspCI CTTAAG 2 cut(s) 905, 1193
MspI CCGG 6 cut(s) 179, 766, 1426, 1471, 1643, 1687
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 5 cut(s) 298, 606, 669, 1512, 1629
MwoI GCNNNNNNNGC 2 cut(s) 1025, 1187
NciI CCSGG 5 cut(s) 766, 1426, 1472, 1643, 1688
NdeII GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
NlaIII CATG 5 cut(s) 44, 422, 457, 1582, 1681
NlaIV GGNNCC 3 cut(s) 462, 483, 1130
NmuCI GTSAC 5 cut(s) 163, 227, 772, 1493, 1651
NsbI TGCGCA 1 cut(s) 633
NspI RCATGY 1 cut(s) 44
NspV TTCGAA 1 cut(s) 1327
PagI TCATGA 1 cut(s) 418
PceI AGGCCT 1 cut(s) 856
PciI ACATGT 1 cut(s) 40
PctI GAATGC 1 cut(s) 124
PfeI GAWTC 5 cut(s) 172, 472, 601, 1057, 1295
PfoI TCCNGGA 1 cut(s) 764
PkrI GCNGC 4 cut(s) 631, 649, 652, 895
PleI GAGTC 2 cut(s) 308, 892
PpsI GAGTC 2 cut(s) 308, 892
PscI ACATGT 1 cut(s) 40
Psp1406I AACGTT 1 cut(s) 405
Psp6I CCWGG 5 cut(s) 296, 604, 667, 1510, 1627
PspGI CCWGG 5 cut(s) 296, 604, 667, 1510, 1627
PspN4I GGNNCC 3 cut(s) 462, 483, 1130
PspOMI GGGCCC 1 cut(s) 460
PspPI GGNCC 3 cut(s) 460, 461, 671
PsuI RGATCY 2 cut(s) 272, 938
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 2 cut(s) 902, 1023
RsaNI GTAC 2 cut(s) 901, 1022
SaqAI TTAA 6 cut(s) 431, 597, 818, 906, 1194, 1461
SatI GCNGC 4 cut(s) 630, 648, 651, 894
Sau3AI GATC 6 cut(s) 272, 421, 548, 938, 1619, 1681
Sau96I GGNCC 3 cut(s) 460, 461, 671
SchI GAGTC 2 cut(s) 308, 893
SduI GDGCHC 3 cut(s) 345, 464, 1192
SfaNI GCATC 2 cut(s) 373, 687
SfcI CTRYAG 2 cut(s) 232, 942
SfuI TTCGAA 1 cut(s) 1327
SinI GGWCC 1 cut(s) 671
SmlI CTYRAG 4 cut(s) 743, 905, 1111, 1193
SmoI CTYRAG 4 cut(s) 743, 905, 1111, 1193
Sse9I AATT 7 cut(s) 92, 567, 1216, 1238, 1417, 1450, 1523
SseBI AGGCCT 1 cut(s) 856
SsiI CCGC 2 cut(s) 200, 674
SspMI CTAG 6 cut(s) 134, 285, 468, 546, 704, 852
StuI AGGCCT 1 cut(s) 856
TaaI ACNGT 3 cut(s) 685, 1542, 1670
TaiI ACGT 1 cut(s) 408
TaqI TCGA 5 cut(s) 85, 101, 151, 1327, 1490
TasI AATT 7 cut(s) 92, 567, 1216, 1238, 1417, 1450, 1523
TatI WGTACW 1 cut(s) 1021
TfiI GAWTC 5 cut(s) 172, 472, 601, 1057, 1295
Tru1I TTAA 6 cut(s) 431, 597, 818, 906, 1194, 1461
Tru9I TTAA 6 cut(s) 431, 597, 818, 906, 1194, 1461
TscAI CASTG 4 cut(s) 168, 232, 783, 1609
TseFI GTSAC 5 cut(s) 163, 227, 772, 1493, 1651
TseI GCWGC 4 cut(s) 629, 647, 650, 893
Tsp45I GTSAC 5 cut(s) 163, 227, 772, 1493, 1651
TspDTI ATGAA 6 cut(s) 207, 234, 273, 954, 1721, 1736
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 4 cut(s) 168, 232, 783, 1609
Vha464I CTTAAG 2 cut(s) 905, 1193
VpaK11BI GGWCC 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 705
XapI RAATTY 1 cut(s) 1450
XceI RCATGY 1 cut(s) 44
XspI CTAG 6 cut(s) 134, 285, 468, 546, 704, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.