Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0004581

Physical Location & Seq

Genomic Coordinates Reverse (-)

9539870 .. 9542682

Gene Structure 2813 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g13380.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2514 bp

ATGGGATTAGACTCCTTACCCACATCCAGTTTTTCTGAGCCTTACTCTACCCCATATTTTGACACTCAATCTCTCCAAGATGCTCGTTATCACAGGAGAAACGTCGAGTGCTATTATGATGATCAGTTAAGGTATTCTGGAAATCCCATAAAAAGGATGGATGGCCCTACCAGGAATTCTTTGGAGCCAAAGGCCCAGAATGTGCAAAGCACGCCAATTGAGAGGTTCCAGACAGAAACATTGCCTCCTAGATCAGCTAAGTCTATTCCGCTCACACACCATAAACTTTTGTCTCCCATTAAGAATCCCGGCTTTGTTTCCACTAGGAATGCTGCTCACATAATGGAAGCTGCTGCAAAAATTATTGAGCCTGGACCTCCAGCCACCGCCAAGGCCAAGATGCCTTTAGTAGGGGCCTCTTCTGTGCCCCTGAAGGTTCAGGCTCTCAAAGAAAAGGTGGAGGCATCCCGAAAAGTTCCACTGGTTGGGTCTTCTTCAGAAACCTTGAAAGATCAAGATTTAAAAGATAAAGTTGGAGCTGCTTACAAGAAAACGAAGCCTTCTGCGGTGTCCCGAAAGCCTGTTGAATCAAATGCTGCTAAGTCTCTTCAGGGACAGTCTTTGAATAAGAGCTGGAATGGATCTCTCGATTTGTCATTCAGGGCTTCTTCGGAGATGGAAGAAACTAAGGGGAAGTCCATTTCTCTTGCAATCCAAGCAAAGGTCAATGTTCAGAAAAGAGGTCAGAATTTAAGTAGAAATATAAGCTCGGTAGGTCAGAGAGAACAGAATGAGGTCATCTCAAACCAGCCCATCCGGACCCAACCGAATGTTCAAAAGAATCTGCACAAGAAACCTTCTACACATAATGCTTCTGGTGCTCTTAAGCAGAATAACCAGAAACAAAATGGTGTAGTTGATAAAGAGAAATTACCTTCAAAGCCTTTGGTTTCCAACTGGCAGGGTAGAAAAGTGCTGTCTGGGGAATCTTCTTCTGGTCGTCACAAAAGCTCAGTTCGAAGTAGTGGAAATTCCAAAATTGGATCCAGAAAGTTGGGCTCAGAGGCAACGGATAGTGAAAAAGAAGTGTCCTACTCGAACGAAGGGAATTATCCGAGGAAGAAACGATCAATAGATGGAAACTTTCAGTTTAACAAAGATCGTGCTGTTGATGATATGTTGAGTGACAAAAATCAGAAGCGAGTTCAGTCTAATCCAGTTAGTGACAGGCACTATAATTGGGCTGAAGATAGCAGAAAGAAAGGCATGGATGTTGTTTCGTTTACATTCACAGCCCCACTTACACGATCACCGCTTGGCACCGACATCTCAGCTCTGGTTGCGCAGAAGAGCAACAGTCTTTGCACAGATCACCGAGGTAAACGGTTGTTGCTTGACACAGACAGTATGAAGCCATCCTCAGTGGGTTACAATGTGATAGGCGGTGATGCTTTGAGTGCTCTTTTGGAACAAAAGTTAAGGGAATTATCCTATGGAAGTGAATCTTCTAGTCGTGAAGCTGCAAAAGTATGCTCAGCTTCAACTGCTTCAAATTTGGACGTGAAACCCAATTATAATGCAGTTAGCTCCATGCAAAGGTTAAATGACCAGAGAGACCAACATCTGTTGGTTACCGAGAAATCGGGTGATCGATTTGAAGCTGACACTCCTCCTGCATTCAGATTGAAACACAAGTTTCAGGGAGTCGATAAGGCAGACGAATATAGCAGCAGTCCTATTGAAGCTGGACAGGTGCATTGCCGGCATCCTAGTCCAGTCTCTGTTCTGGAATCTTCTTTTTCAAATGAAAGTTATGACTCTTCCATTACCACTGATAGTACAGAAGCAAGCAGGTTGTGCTCATCCGTCCAAGCTCGGGAAGTTAATGTTTTCAGTTGTTCCAAGAAGTTCCATTTAGTAGAAGCTGACACCGAGTTATCAGATTCCGCTTCTTCAACATATACAGGAACTGTGGCAAGAAACCAAGCACTTACAGTTCACATAAAAGAATCTTTGAGATCAAATGAGTGGGAATTGGAATACATAAAGGAGACACTATGTAATGTCGAATTGATGTTTCAAGACTTTACATTGGGCCGGGCCAGGAAGATCATAAACCCTCATCTCTTCAATCTGCTGGAATGTCGAAGAGGACAGTTCGAAATTGATGGTGGAGAGTCCAGGGTAAGGCGAAAGGAACTATTTGATTGTACAAGTGAATGCTTGGACTTGAAATGCAGGCGTTATGTGGGCGGGGGATACAGTGCATGGGTCAAAGGAGTAGCAATGGTGAGGAGAAAGGCGTCTTTAGCCGAAGAAGTGTACAAAGAACTCTCATGTTGGAAAGGCATGTGGGAATGCATGGTGGACGAGCTCGTGGGCAAGGACATGAGCAGCCCCTATGGCAGATGGCTGGACTTCGAAACCGATGCATTTGCACTAGGAGTAGATGTTGAGGGTCAAATATTTGATTCTTTGGTAGATGAAGTGGTTGCTGATATGTTGCAATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

838

Amino Acids

92.83

Weight (kDa)

8.98

Isoelectric Point (pI)

49.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05750 AT3G05750 AT3G05750 AT3G05750 AT3G58650 AT5G26910 AT5G26910 AT5G26910 AT5G26910
fragaria_vesca	FvH4_4g19300 FvH4_4g19300 FvH4_4g19300
malus_domestica	MD13G1158100.v1.1 MD16G1158900.v1.1
prunus_persica	Prupe.1G125400_v2.0.a1 Prupe.1G125400_v2.0.a1 Prupe.1G125400_v2.0.a1
pyrus_communis	pycom13g13520 pycom16g13380
rosa_chinensis	RchiOBHm_Chr4g0424321
rosa_laevigata	RLG00000007486
rosa_multiflora	Rmu_sc0002260.1_g000005
rosa_roxburghii	Rroxscaffold_5G00366270
rosa_rugosa	Rorug04G0195900 Rorug04G0195900
rosa_samantha	Rh4AG252700 Rh4BG258100 Rh4CG269000 Rh4DG252400 Rh4DG252500
rosa_wichuraiana	Rw4G021790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1575
Acc16I	TGCGCA	1 cut(s)	1344
Acc36I	ACCTGC	1 cut(s)	1842
AccB1I	GGYRCC	1 cut(s)	1319
AccB7I	CCANNNNNTGG	1 cut(s)	485
AccBSI	CCGCTC	1 cut(s)	271
AccIII	TCCGGA	1 cut(s)	816
AciI	CCGC	7 cut(s)	269, 387, 566, 1313, 1443, 1947, 2253
AclWI	GGATC	3 cut(s)	649, 1038, 1051
AcsI	RAATTY	4 cut(s)	175, 748, 1030, 1552
AcuI	CTGAAG	4 cut(s)	452, 480, 593, 1266
AcyI	GRCGYC	1 cut(s)	2303
AfaI	GTAC	3 cut(s)	1840, 2212, 2324
AfiI	CCNNNNNNNGG	7 cut(s)	153, 410, 485, 721, 1596, 1876, 2187
AflII	CTTAAG	1 cut(s)	884
AjiI	CACGTC	1 cut(s)	1561
AjnI	CCWGG	4 cut(s)	170, 370, 2102, 2180
AjuI	GAANNNNNNNTTGG	2 cut(s)	816, 848
Alw21I	GWGCWC	4 cut(s)	883, 1462, 1862, 2376
Alw26I	GTCTC	5 cut(s)	297, 609, 1608, 1783, 2045
AlwI	GGATC	3 cut(s)	649, 1038, 1051
AlwNI	CAGNNNCTG	2 cut(s)	1781, 1970
Ama87I	CYCGRG	1 cut(s)	1875
Aor13HI	TCCGGA	1 cut(s)	816
AoxI	GGCC	6 cut(s)	163, 192, 393, 414, 2095, 2100
ApeKI	GCWGC	8 cut(s)	332, 350, 353, 539, 596, 1520, 1728, 2394
ApoI	RAATTY	4 cut(s)	175, 748, 1030, 1552
AspLEI	GCGC	1 cut(s)	1345
AspS9I	GGNCC	7 cut(s)	164, 193, 374, 414, 819, 2095, 2100
AsuC2I	CCSGG	2 cut(s)	309, 2099
AsuHPI	GGTGA	5 cut(s)	1302, 1364, 1457, 1658, 2302
AsuII	TTCGAA	3 cut(s)	1018, 2160, 2421
AvaI	CYCGRG	1 cut(s)	1875
AvaII	GGWCC	2 cut(s)	374, 819
BaeGI	GKGCMC	1 cut(s)	429
BamHI	GGATCC	1 cut(s)	1043
BanI	GGYRCC	1 cut(s)	1319
BanII	GRGCYC	2 cut(s)	1061, 2376
BauI	CACGAG	1 cut(s)	2375
BbsI	GAAGAC	1 cut(s)	483
Bbv12I	GWGCWC	4 cut(s)	883, 1462, 1862, 2376
BbvI	GCAGC	8 cut(s)	319, 337, 340, 526, 583, 1507, 1740, 2406
BccI	CCATC	8 cut(s)	151, 155, 670, 821, 1130, 1423, 2162, 2403
BcgI	CGANNNNNNTGC	4 cut(s)	2411, 2417, 2445, 2451
BciT130I	CCWGG	4 cut(s)	172, 372, 2104, 2182
BciVI	GTATCC	1 cut(s)	2252
BclI	TGATCA	1 cut(s)	121
BcnI	CCSGG	2 cut(s)	309, 2099
BcoDI	GTCTC	5 cut(s)	297, 609, 1608, 1783, 2045
BfaI	CTAG	5 cut(s)	249, 324, 1509, 1770, 2441
BfrI	CTTAAG	1 cut(s)	884
BfuAI	ACCTGC	1 cut(s)	1842
BfuI	GTATCC	1 cut(s)	2252
BglI	GCCNNNNNGGC	1 cut(s)	2403
BisI	GCNGC	8 cut(s)	333, 351, 354, 540, 597, 1521, 1729, 2395
BlpI	GCTNAGC	1 cut(s)	1534
BlsI	GCNGC	8 cut(s)	334, 352, 355, 541, 598, 1522, 1730, 2396
Bme1390I	CCNGG	6 cut(s)	172, 309, 372, 2099, 2104, 2182
Bme18I	GGWCC	2 cut(s)	374, 819
BmeT110I	CYCGRG	1 cut(s)	1875
BmgBI	CACGTC	1 cut(s)	1561
BmgT120I	GGNCC	7 cut(s)	164, 193, 374, 414, 819, 2095, 2100
BmiI	GGNNCC	6 cut(s)	186, 227, 415, 821, 1045, 1321
BmrFI	CCNGG	6 cut(s)	172, 309, 372, 2099, 2104, 2182
BmsI	GCATC	6 cut(s)	70, 390, 473, 1438, 1774, 2419
BpiI	GAAGAC	1 cut(s)	483
BplI	GAGNNNNNCTC	4 cut(s)	29, 61, 785, 817
BpmI	CTGGAG	1 cut(s)	363
Bpu1102I	GCTNAGC	1 cut(s)	1534
Bpu14I	TTCGAA	3 cut(s)	1018, 2160, 2421
BpuMI	CCSGG	2 cut(s)	309, 2099
Bsa29I	ATCGAT	1 cut(s)	1651
BsaHI	GRCGYC	1 cut(s)	2303
BsaI	GGTCTC	1 cut(s)	1608
BsaJI	CCNNGG	4 cut(s)	390, 1115, 1375, 2181
BsaWI	WCCGGW	1 cut(s)	816
BsaXI	ACNNNNNCTCC	4 cut(s)	229, 259, 2436, 2466
Bsc4I	CCNNNNNNNGG	7 cut(s)	153, 410, 485, 721, 1596, 1876, 2187
Bse118I	RCCGGY	1 cut(s)	1761
Bse1I	ACTGG	5 cut(s)	27, 486, 962, 1217, 1775
Bse3DI	GCAATG	3 cut(s)	239, 1756, 2292
BseAI	TCCGGA	1 cut(s)	816
BseBI	CCWGG	4 cut(s)	172, 372, 2104, 2182
BseCI	ATCGAT	1 cut(s)	1651
BseDI	CCNNGG	4 cut(s)	390, 1115, 1375, 2181
BseGI	GGATG	9 cut(s)	23, 162, 166, 464, 813, 1276, 1415, 1765, 1862
BseLI	CCNNNNNNNGG	7 cut(s)	153, 410, 485, 721, 1596, 1876, 2187
BseMI	GCAATG	3 cut(s)	239, 1756, 2292
BseMII	CTCAG	6 cut(s)	27, 1026, 1074, 1344, 1434, 1548
BseNI	ACTGG	5 cut(s)	27, 486, 962, 1217, 1775
BseRI	GAGGAG	2 cut(s)	1659, 2308
BseSI	GKGCMC	1 cut(s)	429
BseXI	GCAGC	8 cut(s)	319, 337, 340, 526, 583, 1507, 1740, 2406
BsgI	GTGCAG	1 cut(s)	830
BshFI	GGCC	6 cut(s)	165, 194, 395, 416, 2097, 2102
BshNI	GGYRCC	1 cut(s)	1319
BshVI	ATCGAT	1 cut(s)	1651
BsiHKAI	GWGCWC	4 cut(s)	883, 1462, 1862, 2376
BsiHKCI	CYCGRG	1 cut(s)	1875
BsiSI	CCGG	4 cut(s)	309, 817, 1762, 2098
BslFI	GGGAC	2 cut(s)	556, 627
BslI	CCNNNNNNNGG	7 cut(s)	153, 410, 485, 721, 1596, 1876, 2187
BsmAI	GTCTC	5 cut(s)	297, 609, 1608, 1783, 2045
BsmFI	GGGAC	2 cut(s)	556, 627
BsmI	GAATGC	4 cut(s)	334, 1676, 2225, 2363
BsnI	GGCC	6 cut(s)	165, 194, 395, 416, 2097, 2102
Bso31I	GGTCTC	1 cut(s)	1608
BsoBI	CYCGRG	1 cut(s)	1875
Bsp119I	TTCGAA	3 cut(s)	1018, 2160, 2421
Bsp1286I	GDGCHC	6 cut(s)	429, 883, 1061, 1462, 1862, 2376
Bsp13I	TCCGGA	1 cut(s)	816
Bsp1407I	TGTACA	2 cut(s)	2210, 2322
Bsp1720I	GCTNAGC	1 cut(s)	1534
BspACI	CCGC	7 cut(s)	269, 387, 566, 1313, 1443, 1947, 2253
BspANI	GGCC	6 cut(s)	165, 194, 395, 416, 2097, 2102
BspCNI	CTCAG	6 cut(s)	28, 1025, 1073, 1343, 1433, 1547
BspDI	ATCGAT	1 cut(s)	1651
BspEI	TCCGGA	1 cut(s)	816
BspLI	GGNNCC	6 cut(s)	186, 227, 415, 821, 1045, 1321
BspMI	ACCTGC	1 cut(s)	1842
BspPI	GGATC	3 cut(s)	649, 1038, 1051
BspQI	GCTCTTC	1 cut(s)	1343
BspT104I	TTCGAA	3 cut(s)	1018, 2160, 2421
BspT107I	GGYRCC	1 cut(s)	1319
BspTI	CTTAAG	1 cut(s)	884
BspTNI	GGTCTC	1 cut(s)	1608
BsrBI	CCGCTC	1 cut(s)	271
BsrDI	GCAATG	3 cut(s)	239, 1756, 2292
BsrFI	RCCGGY	1 cut(s)	1761
BsrGI	TGTACA	2 cut(s)	2210, 2322
BsrI	ACTGG	5 cut(s)	27, 486, 962, 1217, 1775
BssAI	RCCGGY	1 cut(s)	1761
BssECI	CCNNGG	4 cut(s)	390, 1115, 1375, 2181
BssNI	GRCGYC	1 cut(s)	2303
BssSI	CACGAG	1 cut(s)	2375
BssT1I	CCWWGG	1 cut(s)	390
Bst2BI	CACGAG	1 cut(s)	2375
Bst2UI	CCWGG	4 cut(s)	172, 372, 2104, 2182
Bst4CI	ACNGT	8 cut(s)	618, 1358, 1386, 1406, 1972, 1996, 2157, 2264
Bst6I	CTCTTC	6 cut(s)	424, 612, 1343, 1825, 2132, 2143
BstACI	GRCGYC	1 cut(s)	2303
BstAFI	CTTAAG	1 cut(s)	884
BstAPI	GCANNNNNTGC	1 cut(s)	1857
BstAUI	TGTACA	2 cut(s)	2210, 2322
BstBI	TTCGAA	3 cut(s)	1018, 2160, 2421
BstC8I	GCNNGC	4 cut(s)	212, 1763, 1849, 2240
BstDEI	CTNAG	9 cut(s)	36, 258, 600, 687, 1012, 1060, 1330, 1420, 1534
BstEII	GGTNACC	1 cut(s)	1630
BstENI	CCTNNNNNAGG	1 cut(s)	408
BstF5I	GGATG	9 cut(s)	23, 162, 166, 464, 813, 1276, 1415, 1765, 1862
BstHHI	GCGC	1 cut(s)	1345
BstMAI	GTCTC	5 cut(s)	297, 609, 1608, 1783, 2045
BstNI	CCWGG	4 cut(s)	172, 372, 2104, 2182
BstNSI	RCATGY	1 cut(s)	2353
BstPI	GGTNACC	1 cut(s)	1630
BstSCI	CCNGG	6 cut(s)	170, 307, 370, 2097, 2102, 2180
BstSLI	GKGCMC	1 cut(s)	429
BstV1I	GCAGC	8 cut(s)	319, 337, 340, 526, 583, 1507, 1740, 2406
BstV2I	GAAGAC	1 cut(s)	483
BstX2I	RGATCY	2 cut(s)	641, 1043
BstXI	CCANNNNNNTGG	1 cut(s)	1054
BstYI	RGATCY	2 cut(s)	641, 1043
Bsu15I	ATCGAT	1 cut(s)	1651
BsuI	GTATCC	1 cut(s)	2252
BsuRI	GGCC	6 cut(s)	165, 194, 395, 416, 2097, 2102
BsuTUI	ATCGAT	1 cut(s)	1651
BtrI	CACGTC	1 cut(s)	1561
BtsCI	GGATG	9 cut(s)	23, 162, 166, 464, 813, 1276, 1415, 1765, 1862
BtsIMutI	CAGTG	4 cut(s)	479, 1428, 1830, 2269
BveI	ACCTGC	1 cut(s)	1842
Cac8I	GCNNGC	4 cut(s)	212, 1763, 1849, 2240
CaiI	CAGNNNCTG	2 cut(s)	1781, 1970
CfoI	GCGC	1 cut(s)	1345
Cfr10I	RCCGGY	1 cut(s)	1761
Cfr13I	GGNCC	7 cut(s)	164, 193, 374, 414, 819, 2095, 2100
ClaI	ATCGAT	1 cut(s)	1651
CseI	GACGC	1 cut(s)	2292
Csp6I	GTAC	3 cut(s)	1839, 2211, 2323
CviAII	CATG	7 cut(s)	1267, 1591, 2268, 2337, 2350, 2362, 2389
CviQI	GTAC	3 cut(s)	1839, 2211, 2323
DdeI	CTNAG	9 cut(s)	36, 258, 600, 687, 1012, 1060, 1330, 1420, 1534
DraI	TTTAAA	1 cut(s)	522
Eam1104I	CTCTTC	6 cut(s)	424, 612, 1343, 1825, 2132, 2143
EarI	CTCTTC	6 cut(s)	424, 612, 1343, 1825, 2132, 2143
Ecl136II	GAGCTC	1 cut(s)	2374
Eco130I	CCWWGG	1 cut(s)	390
Eco24I	GRGCYC	2 cut(s)	1061, 2376
Eco31I	GGTCTC	1 cut(s)	1608
Eco47I	GGWCC	2 cut(s)	374, 819
Eco53kI	GAGCTC	1 cut(s)	2374
Eco57I	CTGAAG	4 cut(s)	452, 480, 593, 1266
Eco88I	CYCGRG	1 cut(s)	1875
Eco91I	GGTNACC	1 cut(s)	1630
EcoICRI	GAGCTC	1 cut(s)	2374
EcoNI	CCTNNNNNAGG	1 cut(s)	408
EcoO109I	RGGNCCY	1 cut(s)	414
EcoO65I	GGTNACC	1 cut(s)	1630
EcoRI	GAATTC	1 cut(s)	175
EcoRII	CCWGG	4 cut(s)	170, 370, 2102, 2180
EcoT14I	CCWWGG	1 cut(s)	390
EcoT22I	ATGCAT	2 cut(s)	2363, 2434
EcoT38I	GRGCYC	2 cut(s)	1061, 2376
ErhI	CCWWGG	1 cut(s)	390
FaeI	CATG	7 cut(s)	1270, 1594, 2271, 2340, 2353, 2365, 2392
FalI	AAGNNNNNCTT	2 cut(s)	1489, 1521
FaqI	GGGAC	2 cut(s)	556, 627
FatI	CATG	7 cut(s)	1266, 1590, 2267, 2336, 2349, 2361, 2388
FauI	CCCGC	1 cut(s)	2246
FbaI	TGATCA	1 cut(s)	121
Fnu4HI	GCNGC	8 cut(s)	333, 351, 354, 540, 597, 1521, 1729, 2395
FokI	GGATG	9 cut(s)	10, 169, 173, 451, 800, 1283, 1402, 1752, 1849
FriOI	GRGCYC	2 cut(s)	1061, 2376
Fsp4HI	GCNGC	8 cut(s)	333, 351, 354, 540, 597, 1521, 1729, 2395
FspBI	CTAG	5 cut(s)	249, 324, 1509, 1770, 2441
FspI	TGCGCA	1 cut(s)	1344
GlaI	GCGC	1 cut(s)	1344
GluI	GCNGC	8 cut(s)	333, 351, 354, 540, 597, 1521, 1729, 2395
GsuI	CTGGAG	1 cut(s)	363
HaeIII	GGCC	6 cut(s)	165, 194, 395, 416, 2097, 2102
HapII	CCGG	4 cut(s)	309, 817, 1762, 2098
HgaI	GACGC	1 cut(s)	2292
HhaI	GCGC	1 cut(s)	1345
Hin1I	GRCGYC	1 cut(s)	2303
Hin1II	CATG	7 cut(s)	1270, 1594, 2271, 2340, 2353, 2365, 2392
Hin6I	GCGC	1 cut(s)	1343
HinP1I	GCGC	1 cut(s)	1343
HpaII	CCGG	4 cut(s)	309, 817, 1762, 2098
HphI	GGTGA	5 cut(s)	1302, 1364, 1457, 1658, 2302
Hpy166II	GTNNAC	5 cut(s)	1284, 1382, 1999, 2323, 2368
Hpy8I	GTNNAC	5 cut(s)	1284, 1382, 1999, 2323, 2368
Hpy99I	CGWCG	1 cut(s)	107
HpyAV	CCTTC	5 cut(s)	427, 570, 867, 945, 1097
HpyCH4III	ACNGT	8 cut(s)	618, 1358, 1386, 1406, 1972, 1996, 2157, 2264
HpyCH4IV	ACGT	2 cut(s)	102, 1560
HpyF3I	CTNAG	9 cut(s)	36, 258, 600, 687, 1012, 1060, 1330, 1420, 1534
HpySE526I	ACGT	2 cut(s)	102, 1560
Hsp92I	GRCGYC	1 cut(s)	2303
Hsp92II	CATG	7 cut(s)	1270, 1594, 2271, 2340, 2353, 2365, 2392
HspAI	GCGC	1 cut(s)	1343
Kpn2I	TCCGGA	1 cut(s)	816
KroI	GCCGGC	1 cut(s)	1761
KroNI	GCCGGC	1 cut(s)	1763
Ksp22I	TGATCA	1 cut(s)	121
LguI	GCTCTTC	1 cut(s)	1343
LmnI	GCTCC	3 cut(s)	184, 536, 1592
Lsp1109I	GCAGC	8 cut(s)	319, 337, 340, 526, 583, 1507, 1740, 2406
LweI	GCATC	6 cut(s)	70, 390, 473, 1438, 1774, 2419
MaeI	CTAG	5 cut(s)	249, 324, 1509, 1770, 2441
MaeII	ACGT	2 cut(s)	102, 1560
MaeIII	GTNAC	5 cut(s)	1001, 1184, 1223, 1427, 1630
MbiI	CCGCTC	1 cut(s)	271
MfeI	CAATTG	2 cut(s)	216, 2507
MflI	RGATCY	2 cut(s)	641, 1043
MhlI	GDGCHC	6 cut(s)	429, 883, 1061, 1462, 1862, 2376
MlyI	GAGTC	4 cut(s)	5, 1713, 1811, 2186
MmeI	TCCRAC	3 cut(s)	514, 978, 2321
Mph1103I	ATGCAT	2 cut(s)	2363, 2434
MroI	TCCGGA	1 cut(s)	816
MroNI	GCCGGC	1 cut(s)	1761
MseI	TTAA	9 cut(s)	128, 300, 521, 752, 885, 1152, 1478, 1601, 1884
MspCI	CTTAAG	1 cut(s)	884
MspI	CCGG	4 cut(s)	309, 817, 1762, 2098
MspR9I	CCNGG	6 cut(s)	172, 309, 372, 2099, 2104, 2182
MunI	CAATTG	2 cut(s)	216, 2507
Mva1269I	GAATGC	4 cut(s)	334, 1676, 2225, 2363
MvaI	CCWGG	4 cut(s)	172, 372, 2104, 2182
NaeI	GCCGGC	1 cut(s)	1763
NciI	CCSGG	2 cut(s)	309, 2099
NgoMIV	GCCGGC	1 cut(s)	1761
NlaIII	CATG	7 cut(s)	1270, 1594, 2271, 2340, 2353, 2365, 2392
NlaIV	GGNNCC	6 cut(s)	186, 227, 415, 821, 1045, 1321
NmuCI	GTSAC	3 cut(s)	1001, 1184, 1223
NsbI	TGCGCA	1 cut(s)	1344
NsiI	ATGCAT	2 cut(s)	2363, 2434
NspI	RCATGY	1 cut(s)	2353
NspV	TTCGAA	3 cut(s)	1018, 2160, 2421
PciSI	GCTCTTC	1 cut(s)	1343
PctI	GAATGC	4 cut(s)	334, 1676, 2225, 2363
PdiI	GCCGGC	1 cut(s)	1763
PfeI	GAWTC	9 cut(s)	304, 587, 841, 986, 1502, 1790, 1943, 2009, 2471
PflMI	CCANNNNNTGG	1 cut(s)	485
PkrI	GCNGC	8 cut(s)	334, 352, 355, 541, 598, 1522, 1730, 2396
PleI	GAGTC	4 cut(s)	5, 1712, 1811, 2185
PpsI	GAGTC	4 cut(s)	5, 1712, 1811, 2185
PsiI	TTATAA	1 cut(s)	1575
Psp124BI	GAGCTC	1 cut(s)	2376
Psp6I	CCWGG	4 cut(s)	170, 370, 2102, 2180
PspEI	GGTNACC	1 cut(s)	1630
PspGI	CCWGG	4 cut(s)	170, 370, 2102, 2180
PspN4I	GGNNCC	6 cut(s)	186, 227, 415, 821, 1045, 1321
PspPI	GGNCC	7 cut(s)	164, 193, 374, 414, 819, 2095, 2100
PstNI	CAGNNNCTG	2 cut(s)	1781, 1970
PsuI	RGATCY	2 cut(s)	641, 1043
RsaI	GTAC	3 cut(s)	1840, 2212, 2324
RsaNI	GTAC	3 cut(s)	1839, 2211, 2323
SacI	GAGCTC	1 cut(s)	2376
SapI	GCTCTTC	1 cut(s)	1343
SaqAI	TTAA	9 cut(s)	128, 300, 521, 752, 885, 1152, 1478, 1601, 1884
SatI	GCNGC	8 cut(s)	333, 351, 354, 540, 597, 1521, 1729, 2395
Sau96I	GGNCC	7 cut(s)	164, 193, 374, 414, 819, 2095, 2100
SchI	GAGTC	4 cut(s)	5, 1713, 1811, 2186
ScrFI	CCNGG	6 cut(s)	172, 309, 372, 2099, 2104, 2182
SduI	GDGCHC	6 cut(s)	429, 883, 1061, 1462, 1862, 2376
SfaNI	GCATC	6 cut(s)	70, 390, 473, 1438, 1774, 2419
SfuI	TTCGAA	3 cut(s)	1018, 2160, 2421
SinI	GGWCC	2 cut(s)	374, 819
SmlI	CTYRAG	1 cut(s)	884
SmoI	CTYRAG	1 cut(s)	884
SsiI	CCGC	7 cut(s)	269, 387, 566, 1313, 1443, 1947, 2253
SspI	AATATT	1 cut(s)	2466
SspMI	CTAG	5 cut(s)	249, 324, 1509, 1770, 2441
SstI	GAGCTC	1 cut(s)	2376
StyD4I	CCNGG	6 cut(s)	170, 307, 370, 2097, 2102, 2180
StyI	CCWWGG	1 cut(s)	390
TaaI	ACNGT	8 cut(s)	618, 1358, 1386, 1406, 1972, 1996, 2157, 2264
TaiI	ACGT	2 cut(s)	105, 1563
TatI	WGTACW	3 cut(s)	1838, 2210, 2322
TfiI	GAWTC	9 cut(s)	304, 587, 841, 986, 1502, 1790, 1943, 2009, 2471
Tru1I	TTAA	9 cut(s)	128, 300, 521, 752, 885, 1152, 1478, 1601, 1884
Tru9I	TTAA	9 cut(s)	128, 300, 521, 752, 885, 1152, 1478, 1601, 1884
TscAI	CASTG	4 cut(s)	486, 1428, 1837, 2269
TseFI	GTSAC	3 cut(s)	1001, 1184, 1223
TseI	GCWGC	8 cut(s)	332, 350, 353, 539, 596, 1520, 1728, 2394
Tsp45I	GTSAC	3 cut(s)	1001, 1184, 1223
TspDTI	ATGAA	3 cut(s)	1424, 1821, 2499
TspGWI	ACGGA	2 cut(s)	1085, 1855
TspRI	CASTG	4 cut(s)	486, 1428, 1837, 2269
Van91I	CCANNNNNTGG	1 cut(s)	485
Vha464I	CTTAAG	1 cut(s)	884
VpaK11BI	GGWCC	2 cut(s)	374, 819
XagI	CCTNNNNNAGG	1 cut(s)	408
XapI	RAATTY	4 cut(s)	175, 748, 1030, 1552
XceI	RCATGY	1 cut(s)	2353
XcmI	CCANNNNNNNNNTGG	3 cut(s)	154, 178, 905
XspI	CTAG	5 cut(s)	249, 324, 1509, 1770, 2441
Zsp2I	ATGCAT	2 cut(s)	2363, 2434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.