Rh4DG252500

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004581
Physical Location & Seq
Forward (+)
46773011 .. 46774326
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG252500.1

Sequence Viewer

Length: 1077 bp
ATGCTTTTGGAACAAAAGTTGAAAGAATTGTCTTATGGAACTGAATCTTCTAGTCACGACTCCATGAAAGTAGGATCAAGTTCAACTGCTTCTAATGTGGATGTGGACTCCAATTTTAATGCAGCTAGTGCTGTGAAAAGACTAAATGAAGAGAGAGACAAACGTCTGTTTGTTTCAGACAAATTGGGTGGCCTGTATGACTCTGAATTGTCATCCACTGATCGTGCTGCTTTCAGATCTAAGCAGAAGTTTCAGGGAGTTGATAAGGTGGACGAGTATCGCAGCAGTCATATTGAATCTGGAAAGACGCTTAATTGCCGACATCCTAGTCCAGTCTCAATTTTGGAACCCACTTTTTCAAATGAAAGTTACGACTCTTCAGTTAGCAGTGATAGTAATAGCACAGAAGCAAGCAAGTTATGTTCTTCCATTCGAGCTCAAGAAGTCGATGGTTTGTGTTCTTCAAAGAAGTTCCACTCTGTAGAAGTTGATACCGAGTTATCAGATTCTGCTTCTTCCACCTCCACTGGAACTGTGGCAAGAAACCATGCAGCTACAGTATACATGCTGAATCCTTGGAGATCAGATGAATGGGAATTGGGGTACATTAAGGAGACACTGTGTAATGTGGAATTCATGTTTCAAGACTTGGCATTGGGTCGATCCCACGAGATCATAAACCCTCATCTGTTCAATCTGTTGGAGAGTCGAAGAGGACGGTTTGAAAAAGATGGTAGCAAGTCCAGGCTAAGGCGAAAGGAACTATTTGATTGTACAAGTGAACGCTTGGACTTTCGATGCAGGCGTTTTGTGGGTGGGGGATACAAAGCATGGGTTAAAGGCACAGCAATGGTGAGAAGAAAGGACATCTTAGCAGAAGAATTCTACAAAGAATTCTCAGCTTTGACTAGCATGGGGGATTGTATGGTGGATGAGCTCGTGGCCAAGGACATGACCAGCCAGTATGGAAGATGGCTTGAATTTGAAACTGATGAATTTTCACTCGGAGTTGAGATTGAGGGTCAAATATTTAGTTCTTTGGTCGATGAAGTGGTTGCTGATATCCTGAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

358

Amino Acids

40.3

Weight (kDa)

5.25

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 200 - 350 3.5e-30 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1075
AasI GACNNNNNNGTC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 561
AclWI GGATC 2 cut(s) 82, 657
AcoI YGGCCR 1 cut(s) 942
AcsI RAATTY 5 cut(s) 632, 881, 893, 980, 995
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 2 cut(s) 605, 775
AfiI CCNNNNNNNGG 1 cut(s) 750
AjnI CCWGG 1 cut(s) 743
AluBI AGCT 5 cut(s) 125, 437, 554, 902, 937
AluI AGCT 5 cut(s) 125, 437, 554, 902, 937
Alw21I GWGCWC 2 cut(s) 439, 939
Alw26I GTCTC 3 cut(s) 150, 340, 608
AlwI GGATC 2 cut(s) 82, 657
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 2 cut(s) 190, 942
ApeKI GCWGC 4 cut(s) 122, 227, 282, 551
ApoI RAATTY 5 cut(s) 632, 881, 893, 980, 995
AsuHPI GGTGA 1 cut(s) 865
BalI TGGCCA 1 cut(s) 944
BanII GRGCYC 2 cut(s) 439, 939
BauI CACGAG 2 cut(s) 668, 938
Bbv12I GWGCWC 2 cut(s) 439, 939
BbvI GCAGC 4 cut(s) 134, 214, 294, 563
BccI CCATC 3 cut(s) 443, 725, 966
BciT130I CCWGG 1 cut(s) 745
BciVI GTATCC 1 cut(s) 815
BcoDI GTCTC 3 cut(s) 150, 340, 608
BfaI CTAG 4 cut(s) 51, 126, 327, 909
BfmI CTRYAG 2 cut(s) 480, 555
BfuI GTATCC 1 cut(s) 815
BglII AGATCT 1 cut(s) 236
BisI GCNGC 4 cut(s) 123, 228, 283, 552
BlsI GCNGC 4 cut(s) 124, 229, 284, 553
Bme1390I CCNGG 1 cut(s) 745
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 1 cut(s) 745
BmsI GCATC 1 cut(s) 788
BoxI GACNNNNGTC 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 749
BpuEI CTTGAG 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 575, 945
BsaXI ACNNNNNCTCC 2 cut(s) 605, 635
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 3 cut(s) 332, 532, 961
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 575, 945
BseGI GGATG 4 cut(s) 106, 212, 322, 937
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 3 cut(s) 332, 532, 961
BseXI GCAGC 4 cut(s) 134, 214, 294, 563
BshFI GGCC 2 cut(s) 192, 944
BsiHKAI GWGCWC 2 cut(s) 439, 939
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 3 cut(s) 150, 340, 608
BsnI GGCC 2 cut(s) 192, 944
Bsp1286I GDGCHC 2 cut(s) 439, 939
Bsp1407I TGTACA 1 cut(s) 773
Bsp143I GATC 6 cut(s) 74, 220, 236, 581, 662, 672
BspANI GGCC 2 cut(s) 192, 944
BspCNI CTCAG 1 cut(s) 911
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 2 cut(s) 82, 657
BsrDI GCAATG 1 cut(s) 855
BsrGI TGTACA 1 cut(s) 773
BsrI ACTGG 3 cut(s) 332, 532, 961
BssECI CCNNGG 2 cut(s) 575, 945
BssMI GATC 6 cut(s) 74, 220, 236, 581, 662, 672
BssNAI GTATAC 1 cut(s) 562
BssSI CACGAG 2 cut(s) 668, 938
BssT1I CCWWGG 2 cut(s) 575, 945
Bst1107I GTATAC 1 cut(s) 562
Bst2BI CACGAG 2 cut(s) 668, 938
Bst2UI CCWGG 1 cut(s) 745
Bst4CI ACNGT 4 cut(s) 535, 559, 621, 720
Bst6I CTCTTC 3 cut(s) 144, 382, 706
BstAPI GCANNNNNTGC 1 cut(s) 128
BstAUI TGTACA 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 412, 803
BstDEI CTNAG 4 cut(s) 240, 749, 871, 898
BstF5I GGATG 4 cut(s) 106, 212, 322, 937
BstKTI GATC 6 cut(s) 77, 223, 239, 584, 665, 675
BstMAI GTCTC 3 cut(s) 150, 340, 608
BstMBI GATC 6 cut(s) 74, 220, 236, 581, 662, 672
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 745
BstNSI RCATGY 1 cut(s) 568
BstPAI GACNNNNGTC 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 743
BstSFI CTRYAG 2 cut(s) 480, 555
BstV1I GCAGC 4 cut(s) 134, 214, 294, 563
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BstZ17I GTATAC 1 cut(s) 562
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 2 cut(s) 192, 944
BtsCI GGATG 4 cut(s) 106, 212, 322, 937
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 4 cut(s) 216, 394, 525, 617
Cac8I GCNNGC 2 cut(s) 412, 803
CaiI CAGNNNCTG 1 cut(s) 509
CseI GACGC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 604, 774
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 7 cut(s) 64, 548, 565, 637, 831, 913, 952
CviQI GTAC 2 cut(s) 604, 774
DdeI CTNAG 4 cut(s) 240, 749, 871, 898
DpnI GATC 6 cut(s) 76, 222, 238, 583, 664, 674
DpnII GATC 6 cut(s) 74, 220, 236, 581, 662, 672
DrdI GACNNNNNNGTC 1 cut(s) 327
DseDI GACNNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 3 cut(s) 144, 382, 706
EarI CTCTTC 3 cut(s) 144, 382, 706
Ecl136II GAGCTC 2 cut(s) 437, 937
Eco130I CCWWGG 2 cut(s) 575, 945
Eco24I GRGCYC 2 cut(s) 439, 939
Eco32I GATATC 1 cut(s) 1063
Eco53kI GAGCTC 2 cut(s) 437, 937
Eco57I CTGAAG 1 cut(s) 363
EcoICRI GAGCTC 2 cut(s) 437, 937
EcoRI GAATTC 3 cut(s) 632, 881, 893
EcoRII CCWGG 1 cut(s) 743
EcoRV GATATC 1 cut(s) 1063
EcoT14I CCWWGG 2 cut(s) 575, 945
EcoT38I GRGCYC 2 cut(s) 439, 939
ErhI CCWWGG 2 cut(s) 575, 945
FaeI CATG 7 cut(s) 67, 551, 568, 640, 834, 916, 955
FalI AAGNNNNNCTT 2 cut(s) 854, 886
FatI CATG 7 cut(s) 63, 547, 564, 636, 830, 912, 951
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 4 cut(s) 123, 228, 283, 552
FokI GGATG 4 cut(s) 113, 199, 309, 944
FriOI GRGCYC 2 cut(s) 439, 939
Fsp4HI GCNGC 4 cut(s) 123, 228, 283, 552
FspBI CTAG 4 cut(s) 51, 126, 327, 909
GluI GCNGC 4 cut(s) 123, 228, 283, 552
HaeIII GGCC 2 cut(s) 192, 944
HgaI GACGC 1 cut(s) 316
Hin1II CATG 7 cut(s) 67, 551, 568, 640, 834, 916, 955
HinfI GANTC 9 cut(s) 44, 59, 107, 200, 296, 374, 506, 571, 706
HphI GGTGA 1 cut(s) 865
Hpy166II GTNNAC 4 cut(s) 106, 271, 562, 782
Hpy188I TCNGA 6 cut(s) 178, 205, 236, 505, 586, 1007
Hpy188III TCNNGA 5 cut(s) 56, 300, 440, 644, 1066
Hpy8I GTNNAC 4 cut(s) 106, 271, 562, 782
HpyCH4III ACNGT 4 cut(s) 535, 559, 621, 720
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 3 cut(s) 122, 551, 801
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 4 cut(s) 240, 749, 871, 898
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 7 cut(s) 67, 551, 568, 640, 834, 916, 955
Kzo9I GATC 6 cut(s) 74, 220, 236, 581, 662, 672
Lsp1109I GCAGC 4 cut(s) 134, 214, 294, 563
LweI GCATC 1 cut(s) 788
MaeI CTAG 4 cut(s) 51, 126, 327, 909
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 2 cut(s) 53, 368
MalI GATC 6 cut(s) 76, 222, 238, 583, 664, 674
MboI GATC 6 cut(s) 74, 220, 236, 581, 662, 672
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 439, 939
MlsI TGGCCA 1 cut(s) 944
MluNI TGGCCA 1 cut(s) 944
MlyI GAGTC 5 cut(s) 53, 101, 194, 368, 715
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 4 cut(s) 532, 693, 707, 1012
Mox20I TGGCCA 1 cut(s) 944
MscI TGGCCA 1 cut(s) 944
MseI TTAA 4 cut(s) 117, 312, 609, 837
MslI CAYNNNNRTG 1 cut(s) 848
Msp20I TGGCCA 1 cut(s) 944
MspR9I CCNGG 1 cut(s) 745
MvaI CCWGG 1 cut(s) 745
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeII GATC 6 cut(s) 74, 220, 236, 581, 662, 672
NlaIII CATG 7 cut(s) 67, 551, 568, 640, 834, 916, 955
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 53
NspI RCATGY 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 802
PfeI GAWTC 4 cut(s) 44, 296, 506, 571
PkrI GCNGC 4 cut(s) 124, 229, 284, 553
PleI GAGTC 5 cut(s) 53, 101, 194, 368, 714
PpsI GAGTC 5 cut(s) 53, 101, 194, 368, 714
PshAI GACNNNNGTC 1 cut(s) 162
PsiI TTATAA 1 cut(s) 1075
Psp124BI GAGCTC 2 cut(s) 439, 939
Psp6I CCWGG 1 cut(s) 743
PspGI CCWGG 1 cut(s) 743
PspN4I GGNNCC 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 2 cut(s) 605, 775
RsaNI GTAC 2 cut(s) 604, 774
RseI CAYNNNNRTG 1 cut(s) 848
SacI GAGCTC 2 cut(s) 439, 939
SaqAI TTAA 4 cut(s) 117, 312, 609, 837
SatI GCNGC 4 cut(s) 123, 228, 283, 552
Sau3AI GATC 6 cut(s) 74, 220, 236, 581, 662, 672
SchI GAGTC 5 cut(s) 53, 101, 194, 368, 715
ScrFI CCNGG 1 cut(s) 745
SduI GDGCHC 2 cut(s) 439, 939
SetI ASST 8 cut(s) 127, 166, 270, 439, 524, 556, 904, 939
SfaNI GCATC 1 cut(s) 788
SfcI CTRYAG 2 cut(s) 480, 555
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 1 cut(s) 438
SmoI CTYRAG 1 cut(s) 438
SspI AATATT 1 cut(s) 1029
SspMI CTAG 4 cut(s) 51, 126, 327, 909
SstI GAGCTC 2 cut(s) 439, 939
StyD4I CCNGG 1 cut(s) 743
StyI CCWWGG 2 cut(s) 575, 945
TaaI ACNGT 4 cut(s) 535, 559, 621, 720
TaiI ACGT 1 cut(s) 166
TaqI TCGA 6 cut(s) 433, 447, 661, 709, 796, 1044
TatI WGTACW 1 cut(s) 773
TfiI GAWTC 4 cut(s) 44, 296, 506, 571
Tru1I TTAA 4 cut(s) 117, 312, 609, 837
Tru9I TTAA 4 cut(s) 117, 312, 609, 837
TscAI CASTG 4 cut(s) 223, 394, 532, 624
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 4 cut(s) 122, 227, 282, 551
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 7 cut(s) 80, 162, 378, 603, 625, 1008, 1062
TspRI CASTG 4 cut(s) 223, 394, 532, 624
XapI RAATTY 5 cut(s) 632, 881, 893, 980, 995
XceI RCATGY 1 cut(s) 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 4 cut(s) 51, 126, 327, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.