MD16G1180700.v1.1

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012389
Physical Location & Seq
Reverse (-)
15520484 .. 15522542
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1180700.v1.1.491

Sequence Viewer

Length: 759 bp
ATGATGAACAAGATGCACGGGTACACGGAGGTGGGTACGAACAGCGGCGGCAAGGCGGCGGAGGTGGACATAGAGTCGGGGGAGTTGCTGTACCCAGGTCTGAGTAAAGGCGAGAACCAACTCCGATGGGGATTCATCCGCAAGGTGTACGGAATCTTGGCCGCTCAGATGGTTTTGACCACTGTGATCTCCTTCGTCACCGTCCTCTACGCTCCGATCAACGATCTCCTCAAGGGCAGCCCTGGAATTCTCCTCTTCTTCGCCATCCTTCCCCTAATCTTGTTGTGGCCCCTGCATGTGTATCAGCAGAAGCATCCCCTCAATTTTGTCTTCCTTGGACTTTTCACTCTGAGTTTGAGTGTCACAGTTGGCGTGGCCGTCGCGAATACAGACGGAGCAATTGTGCTTGAAGCTTTGATTCTAACCTCAGCTGTGGTTGCATCCTTGACTGCTTACACCTTCTGGGCGTCGAGGAAGGGCAAGGACTTCAGCTTCCTCGGACCAGTGTTGTTCACTGGCCTCATTGTCCTCCTCCTAACCGGTTTCATGCAGATGTTCTTCCCTCTTGGCCCTACAACTAATGCCATATATGGTGCCATTGGCGCTATCATTTTCTCTGGGTACATCGTCTATGACACTGACAACCTGATCAAGCGCTTCACATACGATGAGTACATTTGGGCCTCCATCACTCTTTATCTTGACATTCTCAACCTGTTCCTGACTATATTGCGGATGCTGAGGCAAAGCAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bax1-I

Protein Analysis

253

Amino Acids

27.83

Weight (kDa)

6.83

Isoelectric Point (pI)

25.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 44 - 247 3.2e-49 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15470
fragaria_vesca FvH4_4g14100
malus_domestica MD13G1179700.v1.1 MD16G1180700.v1.1
prunus_persica Prupe.1G148200_v2.0.a1 Prupe.1G148200_v2.0.a1
pyrus_communis pycom13g15490 pycom16g15180
rosa_chinensis RchiOBHm_Chr4g0419241
rosa_laevigata RLG00000007822
rosa_multiflora Rmu_sc0004932.1_g000001 Rmu_sc0019148.1_g000001
rosa_roxburghii Rroxscaffold_5G00362220
rosa_rugosa Rorug04G0157600
rosa_samantha Rh4AG219600 Rh4BG221100 Rh4CG232200 Rh4DG218600
rosa_wichuraiana Rw4G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 593
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 1 cut(s) 383
AciI CCGC 7 cut(s) 45, 48, 56, 59, 139, 162, 733
AcoI YGGCCR 2 cut(s) 159, 375
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 472
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 6 cut(s) 23, 37, 92, 149, 623, 674
AfeI AGCGCT 1 cut(s) 656
AgeI ACCGGT 1 cut(s) 539
AgsI TTSAA 1 cut(s) 410
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 2 cut(s) 94, 241
AleI CACNNNNGTG 1 cut(s) 29
AluBI AGCT 3 cut(s) 413, 431, 492
AluI AGCT 3 cut(s) 413, 431, 492
Aor51HI AGCGCT 1 cut(s) 656
AoxI GGCC 6 cut(s) 159, 287, 375, 517, 568, 681
ApeKI GCWGC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 246
AsiGI ACCGGT 1 cut(s) 539
AspLEI GCGC 2 cut(s) 605, 657
AspS9I GGNCC 4 cut(s) 288, 500, 569, 681
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 1 cut(s) 500
BanI GGYRCC 1 cut(s) 593
BbsI GAAGAC 1 cut(s) 322
BbvCI CCTCAGC 2 cut(s) 427, 740
BbvI GCAGC 1 cut(s) 249
BccI CCATC 4 cut(s) 120, 163, 272, 695
BceAI ACGGC 1 cut(s) 362
BciT130I CCWGG 2 cut(s) 96, 243
BclI TGATCA 1 cut(s) 648
BfoI RGCGCY 2 cut(s) 606, 658
BisI GCNGC 5 cut(s) 46, 49, 57, 162, 238
BlsI GCNGC 5 cut(s) 47, 50, 58, 163, 239
Bme1390I CCNGG 2 cut(s) 96, 243
Bme18I GGWCC 1 cut(s) 500
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgT120I GGNCC 4 cut(s) 288, 500, 569, 681
BmiI GGNNCC 2 cut(s) 290, 595
BmrFI CCNGG 2 cut(s) 96, 243
BmsI GCATC 4 cut(s) 3, 322, 449, 726
BpiI GAAGAC 1 cut(s) 322
Bpu10I CCTNAGC 2 cut(s) 427, 740
BpuEI CTTGAG 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 94, 241, 334, 496
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 387, 417
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 2 cut(s) 503, 520
BseBI CCWGG 2 cut(s) 96, 243
BseDI CCNNGG 4 cut(s) 94, 241, 334, 496
BseGI GGATG 5 cut(s) 135, 264, 313, 440, 741
BseMII CTCAG 5 cut(s) 92, 179, 341, 441, 731
BseNI ACTGG 2 cut(s) 503, 520
BseRI GAGGAG 3 cut(s) 218, 242, 521
BseXI GCAGC 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 383
BshFI GGCC 6 cut(s) 161, 289, 377, 519, 570, 683
BshNI GGYRCC 1 cut(s) 593
BshTI ACCGGT 1 cut(s) 539
BsiSI CCGG 1 cut(s) 540
BsnI GGCC 6 cut(s) 161, 289, 377, 519, 570, 683
Bsp143I GATC 4 cut(s) 186, 216, 223, 648
Bsp68I TCGCGA 1 cut(s) 383
BspACI CCGC 7 cut(s) 45, 48, 56, 59, 139, 162, 733
BspANI GGCC 6 cut(s) 161, 289, 377, 519, 570, 683
BspCNI CTCAG 5 cut(s) 93, 178, 342, 440, 732
BspFNI CGCG 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 290, 595
BspT107I GGYRCC 1 cut(s) 593
BsrBI CCGCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 539
BsrI ACTGG 2 cut(s) 503, 520
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 4 cut(s) 94, 241, 334, 496
BssMI GATC 4 cut(s) 186, 216, 223, 648
BssNI GRCGYC 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 334
Bst2UI CCWGG 2 cut(s) 96, 243
Bst4CI ACNGT 3 cut(s) 184, 202, 367
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 467
BstDEI CTNAG 5 cut(s) 101, 165, 350, 427, 740
BstF5I GGATG 5 cut(s) 135, 264, 313, 440, 741
BstFNI CGCG 1 cut(s) 383
BstH2I RGCGCY 2 cut(s) 606, 658
BstHHI GCGC 2 cut(s) 605, 657
BstKTI GATC 4 cut(s) 189, 219, 226, 651
BstMBI GATC 4 cut(s) 186, 216, 223, 648
BstMWI GCNNNNNNNGC 2 cut(s) 437, 602
BstNI CCWGG 2 cut(s) 96, 243
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 94, 241
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 322
BsuRI GGCC 6 cut(s) 161, 289, 377, 519, 570, 683
BtsCI GGATG 5 cut(s) 135, 264, 313, 440, 741
BtsIMutI CAGTG 4 cut(s) 180, 510, 513, 636
BtuMI TCGCGA 1 cut(s) 383
CfoI GCGC 2 cut(s) 605, 657
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 4 cut(s) 288, 500, 569, 681
CseI GACGC 1 cut(s) 456
Csp6I GTAC 6 cut(s) 22, 36, 91, 148, 622, 673
CspAI ACCGGT 1 cut(s) 539
CviAII CATG 2 cut(s) 296, 547
CviQI GTAC 6 cut(s) 22, 36, 91, 148, 622, 673
DdeI CTNAG 5 cut(s) 101, 165, 350, 427, 740
DpnI GATC 4 cut(s) 188, 218, 225, 650
DpnII GATC 4 cut(s) 186, 216, 223, 648
DriI GACNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 2 cut(s) 159, 375
Eam1104I CTCTTC 1 cut(s) 260
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 500
Eco47III AGCGCT 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 472
EcoRI GAATTC 1 cut(s) 246
EcoRII CCWGG 2 cut(s) 94, 241
EcoT14I CCWWGG 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 2 cut(s) 299, 550
FaiI YATR 9 cut(s) 71, 297, 548, 587, 589, 591, 633, 664, 728
FatI CATG 2 cut(s) 295, 546
FbaI TGATCA 1 cut(s) 648
Fnu4HI GCNGC 5 cut(s) 46, 49, 57, 162, 238
FokI GGATG 5 cut(s) 122, 251, 300, 427, 748
Fsp4HI GCNGC 5 cut(s) 46, 49, 57, 162, 238
GlaI GCGC 2 cut(s) 604, 656
GluI GCNGC 5 cut(s) 46, 49, 57, 162, 238
HaeII RGCGCY 2 cut(s) 606, 658
HaeIII GGCC 6 cut(s) 161, 289, 377, 519, 570, 683
HapII CCGG 1 cut(s) 540
HgaI GACGC 1 cut(s) 456
HhaI GCGC 2 cut(s) 605, 657
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 2 cut(s) 299, 550
Hin6I GCGC 2 cut(s) 603, 655
HinP1I GCGC 2 cut(s) 603, 655
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 4 cut(s) 74, 132, 153, 418
HpaII CCGG 1 cut(s) 540
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 4 cut(s) 24, 67, 148, 513
Hpy188I TCNGA 6 cut(s) 102, 125, 168, 216, 351, 500
Hpy188III TCNNGA 3 cut(s) 382, 701, 721
Hpy8I GTNNAC 4 cut(s) 24, 67, 148, 513
Hpy99I CGWCG 2 cut(s) 383, 472
HpyAV CCTTC 4 cut(s) 202, 278, 469, 469
HpyCH4III ACNGT 3 cut(s) 184, 202, 367
HpyCH4V TGCA 4 cut(s) 16, 295, 440, 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 602
HpyF3I CTNAG 5 cut(s) 101, 165, 350, 427, 740
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 2 cut(s) 299, 550
HspAI GCGC 2 cut(s) 603, 655
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 4 cut(s) 186, 216, 223, 648
LmnI GCTCC 2 cut(s) 217, 395
Lsp1109I GCAGC 1 cut(s) 249
LweI GCATC 4 cut(s) 3, 322, 449, 726
MaeIII GTNAC 2 cut(s) 196, 361
MalI GATC 4 cut(s) 188, 218, 225, 650
MbiI CCGCTC 1 cut(s) 164
MboI GATC 4 cut(s) 186, 216, 223, 648
MboII GAAGA 4 cut(s) 247, 250, 322, 550
MfeI CAATTG 1 cut(s) 399
MluCI AATT 4 cut(s) 246, 322, 399, 754
MlyI GAGTC 1 cut(s) 83
MslI CAYNNNNRTG 2 cut(s) 29, 551
MspA1I CMGCKG 2 cut(s) 45, 431
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 2 cut(s) 96, 243
MunI CAATTG 1 cut(s) 399
MvaI CCWGG 2 cut(s) 96, 243
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 437, 602
NdeII GATC 4 cut(s) 186, 216, 223, 648
NlaIII CATG 2 cut(s) 299, 550
NlaIV GGNNCC 2 cut(s) 290, 595
NmuCI GTSAC 2 cut(s) 196, 361
NruI TCGCGA 1 cut(s) 383
NspI RCATGY 1 cut(s) 299
OliI CACNNNNGTG 1 cut(s) 29
PfeI GAWTC 3 cut(s) 132, 153, 418
PinAI ACCGGT 1 cut(s) 539
PkrI GCNGC 5 cut(s) 47, 50, 58, 163, 239
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 94, 241
PspGI CCWGG 2 cut(s) 94, 241
PspN4I GGNNCC 2 cut(s) 290, 595
PspPI GGNCC 4 cut(s) 288, 500, 569, 681
PvuII CAGCTG 1 cut(s) 431
RruI TCGCGA 1 cut(s) 383
RsaI GTAC 6 cut(s) 23, 37, 92, 149, 623, 674
RsaNI GTAC 6 cut(s) 22, 36, 91, 148, 622, 673
RseI CAYNNNNRTG 2 cut(s) 29, 551
SatI GCNGC 5 cut(s) 46, 49, 57, 162, 238
Sau3AI GATC 4 cut(s) 186, 216, 223, 648
Sau96I GGNCC 4 cut(s) 288, 500, 569, 681
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 96, 243
SfaNI GCATC 4 cut(s) 3, 322, 449, 726
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 2 cut(s) 29, 551
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 4 cut(s) 246, 322, 399, 754
SsiI CCGC 7 cut(s) 45, 48, 56, 59, 139, 162, 733
StyD4I CCNGG 2 cut(s) 94, 241
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 3 cut(s) 184, 202, 367
TaqI TCGA 1 cut(s) 470
TasI AATT 4 cut(s) 246, 322, 399, 754
TatI WGTACW 1 cut(s) 672
TauI GCSGC 4 cut(s) 48, 51, 59, 164
TfiI GAWTC 3 cut(s) 132, 153, 418
TscAI CASTG 4 cut(s) 187, 510, 520, 643
TseFI GTSAC 2 cut(s) 196, 361
TseI GCWGC 1 cut(s) 237
Tsp45I GTSAC 2 cut(s) 196, 361
TspDTI ATGAA 3 cut(s) 20, 124, 535
TspGWI ACGGA 3 cut(s) 41, 165, 408
TspRI CASTG 4 cut(s) 187, 510, 520, 643
VpaK11BI GGWCC 1 cut(s) 500
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.