RLG00000007822

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012389
Physical Location & Seq
Reverse (-)
21563637 .. 21565697
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007822

Sequence Viewer

Length: 756 bp
ATGAACACGATGCTCTACACGGAGGTGAGCACCACCGGCGCCGGCGGCAAGCTCGACGAGGTCGACATCGAGTCCGGCGAGGTCTTGTACCCGGGCCTCAGCAAAGGCGAGAACCAGCTCCGTTGGGGTTTCATCCGCAAGGTCTACGGCATCTTATCCGCTCAGCTCCTCCTCACCACCGCCATCTCCTTCGTCACCGTTCTCTACACTCCCGTCAACGATCTCCTCAAGGGCAGCCCCGGCCTTCTCATGTTCTTCGCCTTCCTTCCTTTCGTCTTGTTGTGGCCCCTGCATGTTTACCAGCAAAGGCATCCCCTGAACTTTGTCTTCCTTGGACTTTTCACTGTGAGCCTCAGTGTCACTGTTGGTGTAGCGGTGGCGAATACTGATGGAGCAATAGTGCTTGAAGCATTGATTCTAACCTCTGGTGTGGTTGCATCCTTGACCGCTTACACCTTCTGGGCTTCAAAGAAGGGCAAGGACTTCAGCTTCCTTGGACCAGTCTTGTTCACTGGCCTCATGGTTCTCCTGCTAACCAGCTTCATGCAGATGTTCTTCCCTCTTGGTCCTACGTCTAATGCCGTTATTGGTGGCGTGGGTGCTATTCTTTTCTCTGGGTACATAGTCTATGATACCGAGAACCTCATCAAGCGCTTTACCTATGATGAGTACATCTGGGCCTCCATCACGCTTTATCTGGACATTCTGAACCTGTTCCTCACAATCCTGCGGATGCTGAGGTCGAGCGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bax1-I

Protein Analysis

252

Amino Acids

27.68

Weight (kDa)

5.33

Isoelectric Point (pI)

22.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 43 - 246 1.2e-49 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15470
fragaria_vesca FvH4_4g14100
malus_domestica MD13G1179700.v1.1 MD16G1180700.v1.1
prunus_persica Prupe.1G148200_v2.0.a1 Prupe.1G148200_v2.0.a1
pyrus_communis pycom13g15490 pycom16g15180
rosa_chinensis RchiOBHm_Chr4g0419241
rosa_laevigata RLG00000007822
rosa_multiflora Rmu_sc0004932.1_g000001 Rmu_sc0019148.1_g000001
rosa_roxburghii Rroxscaffold_5G00362220
rosa_rugosa Rorug04G0157600
rosa_samantha Rh4AG219600 Rh4BG221100 Rh4CG232200 Rh4DG218600
rosa_wichuraiana Rw4G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccBSI CCGCTC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 63, 144
AciI CCGC 7 cut(s) 45, 136, 159, 180, 374, 447, 730
AcuI CTGAAG 1 cut(s) 469
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 89, 620, 671
AfeI AGCGCT 1 cut(s) 653
AgsI TTSAA 2 cut(s) 407, 468
AhdI GACNNNNNGTC 1 cut(s) 70
AleI CACNNNNGTG 1 cut(s) 23
AluBI AGCT 5 cut(s) 52, 118, 166, 489, 540
AluI AGCT 5 cut(s) 52, 118, 166, 489, 540
Alw21I GWGCWC 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 91
Aor51HI AGCGCT 1 cut(s) 653
AoxI GGCC 5 cut(s) 94, 241, 284, 514, 678
ApeKI GCWGC 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 2 cut(s) 41, 654
AspS9I GGNCC 5 cut(s) 94, 285, 497, 566, 678
AsuC2I CCSGG 3 cut(s) 92, 93, 240
AsuHPI GGTGA 3 cut(s) 37, 166, 187
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 2 cut(s) 497, 566
BanI GGYRCC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 98, 737
BbvI GCAGC 1 cut(s) 246
BccI CCATC 3 cut(s) 191, 383, 692
BceAI ACGGC 2 cut(s) 163, 566
BcnI CCSGG 3 cut(s) 92, 93, 240
BfoI RGCGCY 2 cut(s) 42, 655
BisI GCNGC 2 cut(s) 46, 235
BlpI GCTNAGC 1 cut(s) 162
BlsI GCNGC 2 cut(s) 47, 236
Bme1390I CCNGG 3 cut(s) 92, 93, 240
Bme18I GGWCC 2 cut(s) 497, 566
BmeRI GACNNNNNGTC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 5 cut(s) 94, 285, 497, 566, 678
BmiI GGNNCC 2 cut(s) 40, 287
BmrFI CCNGG 3 cut(s) 92, 93, 240
BmsI GCATC 4 cut(s) 159, 319, 446, 723
BpiI GAAGAC 1 cut(s) 319
Bpu10I CCTNAGC 2 cut(s) 98, 737
Bpu1102I GCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 3 cut(s) 92, 93, 240
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 91, 238, 331, 493
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bse118I RCCGGY 2 cut(s) 35, 41
Bse1I ACTGG 2 cut(s) 500, 517
BseDI CCNNGG 4 cut(s) 91, 238, 331, 493
BseGI GGATG 4 cut(s) 132, 310, 437, 738
BseMII CTCAG 4 cut(s) 112, 176, 367, 728
BseNI ACTGG 2 cut(s) 500, 517
BseRI GAGGAG 3 cut(s) 158, 161, 215
BseXI GCAGC 1 cut(s) 246
BshFI GGCC 5 cut(s) 96, 243, 286, 516, 680
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 5 cut(s) 36, 42, 75, 92, 240
BsnI GGCC 5 cut(s) 96, 243, 286, 516, 680
BsoBI CYCGRG 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 220
Bsp1720I GCTNAGC 1 cut(s) 162
BspACI CCGC 7 cut(s) 45, 136, 159, 180, 374, 447, 730
BspANI GGCC 5 cut(s) 96, 243, 286, 516, 680
BspCNI CTCAG 4 cut(s) 111, 175, 366, 729
BspLI GGNNCC 2 cut(s) 40, 287
BspT107I GGYRCC 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 161
BsrFI RCCGGY 2 cut(s) 35, 41
BsrI ACTGG 2 cut(s) 500, 517
BssAI RCCGGY 2 cut(s) 35, 41
BssECI CCNNGG 4 cut(s) 91, 238, 331, 493
BssMI GATC 1 cut(s) 220
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 331, 493
Bst4CI ACNGT 3 cut(s) 199, 346, 364
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 43, 50
BstDEI CTNAG 4 cut(s) 98, 162, 353, 737
BstF5I GGATG 4 cut(s) 132, 310, 437, 738
BstH2I RGCGCY 2 cut(s) 42, 655
BstHHI GCGC 2 cut(s) 41, 654
BstKTI GATC 1 cut(s) 223
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 36, 45, 240
BstNSI RCATGY 1 cut(s) 296
BstSCI CCNGG 3 cut(s) 90, 91, 238
BstV1I GCAGC 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 319
BsuRI GGCC 5 cut(s) 96, 243, 286, 516, 680
BtsCI GGATG 4 cut(s) 132, 310, 437, 738
BtsIMutI CAGTG 4 cut(s) 342, 360, 361, 510
Cac8I GCNNGC 2 cut(s) 43, 50
CfoI GCGC 2 cut(s) 41, 654
Cfr10I RCCGGY 2 cut(s) 35, 41
Cfr13I GGNCC 5 cut(s) 94, 285, 497, 566, 678
Cfr9I CCCGGG 1 cut(s) 91
Csp6I GTAC 3 cut(s) 88, 619, 670
CviAII CATG 4 cut(s) 250, 293, 520, 544
CviQI GTAC 3 cut(s) 88, 619, 670
DdeI CTNAG 4 cut(s) 98, 162, 353, 737
DinI GGCGCC 1 cut(s) 40
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 70
Eam1105I GACNNNNNGTC 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 331, 493
Eco47I GGWCC 2 cut(s) 497, 566
Eco47III AGCGCT 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 469
Eco88I CYCGRG 1 cut(s) 91
EcoT14I CCWWGG 2 cut(s) 331, 493
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 2 cut(s) 331, 493
FaeI CATG 4 cut(s) 253, 296, 523, 547
FaiI YATR 7 cut(s) 251, 294, 521, 545, 623, 630, 663
FatI CATG 4 cut(s) 249, 292, 519, 543
FblI GTMKAC 2 cut(s) 63, 144
Fnu4HI GCNGC 2 cut(s) 46, 235
FokI GGATG 4 cut(s) 119, 297, 424, 745
Fsp4HI GCNGC 2 cut(s) 46, 235
GlaI GCGC 2 cut(s) 40, 653
GluI GCNGC 2 cut(s) 46, 235
HaeII RGCGCY 2 cut(s) 42, 655
HaeIII GGCC 5 cut(s) 96, 243, 286, 516, 680
HapII CCGG 5 cut(s) 36, 42, 75, 92, 240
HhaI GCGC 2 cut(s) 41, 654
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 253, 296, 523, 547
Hin6I GCGC 2 cut(s) 39, 652
HinP1I GCGC 2 cut(s) 39, 652
HincII GTYRAC 2 cut(s) 64, 217
HindII GTYRAC 2 cut(s) 64, 217
HinfI GANTC 2 cut(s) 71, 415
HpaII CCGG 5 cut(s) 36, 42, 75, 92, 240
HphI GGTGA 3 cut(s) 37, 166, 187
Hpy166II GTNNAC 5 cut(s) 64, 145, 217, 298, 510
Hpy188I TCNGA 1 cut(s) 708
Hpy188III TCNNGA 1 cut(s) 698
Hpy8I GTNNAC 5 cut(s) 64, 145, 217, 298, 510
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 6 cut(s) 199, 254, 271, 275, 466, 466
HpyCH4III ACNGT 3 cut(s) 199, 346, 364
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 3 cut(s) 292, 437, 547
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 45, 240
HpyF3I CTNAG 4 cut(s) 98, 162, 353, 737
HpySE526I ACGT 1 cut(s) 572
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 253, 296, 523, 547
HspAI GCGC 2 cut(s) 39, 652
KasI GGCGCC 1 cut(s) 38
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Kzo9I GATC 1 cut(s) 220
LmnI GCTCC 3 cut(s) 123, 171, 392
Lsp1109I GCAGC 1 cut(s) 246
LweI GCATC 4 cut(s) 159, 319, 446, 723
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 2 cut(s) 193, 358
MalI GATC 1 cut(s) 222
MbiI CCGCTC 1 cut(s) 161
MboI GATC 1 cut(s) 220
MboII GAAGA 3 cut(s) 247, 319, 547
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 1 cut(s) 751
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 80
MreI CGCCGGCG 1 cut(s) 41
MroNI GCCGGC 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 713
MslI CAYNNNNRTG 2 cut(s) 23, 548
MspI CCGG 5 cut(s) 36, 42, 75, 92, 240
MspR9I CCNGG 3 cut(s) 92, 93, 240
MwoI GCNNNNNNNGC 3 cut(s) 36, 45, 240
NaeI GCCGGC 1 cut(s) 43
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 3 cut(s) 92, 93, 240
NdeII GATC 1 cut(s) 220
NgoMIV GCCGGC 1 cut(s) 41
NlaIII CATG 4 cut(s) 253, 296, 523, 547
NlaIV GGNNCC 2 cut(s) 40, 287
NmuCI GTSAC 2 cut(s) 193, 358
NspI RCATGY 1 cut(s) 296
OliI CACNNNNGTG 1 cut(s) 23
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 75
PdiI GCCGGC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 1 cut(s) 415
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 47, 236
PleI GAGTC 1 cut(s) 79
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 40, 287
PspPI GGNCC 5 cut(s) 94, 285, 497, 566, 678
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 3 cut(s) 89, 620, 671
RsaNI GTAC 3 cut(s) 88, 619, 670
RseI CAYNNNNRTG 2 cut(s) 23, 548
SalI GTCGAC 1 cut(s) 62
SatI GCNGC 2 cut(s) 46, 235
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 5 cut(s) 94, 285, 497, 566, 678
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 3 cut(s) 92, 93, 240
SduI GDGCHC 1 cut(s) 32
SfaNI GCATC 4 cut(s) 159, 319, 446, 723
SfoI GGCGCC 1 cut(s) 40
SgrAI CRCCGGYG 2 cut(s) 35, 41
SinI GGWCC 2 cut(s) 497, 566
SmaI CCCGGG 1 cut(s) 93
SmiMI CAYNNNNRTG 2 cut(s) 23, 548
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 1 cut(s) 751
SsiI CCGC 7 cut(s) 45, 136, 159, 180, 374, 447, 730
SspDI GGCGCC 1 cut(s) 38
StyD4I CCNGG 3 cut(s) 90, 91, 238
StyI CCWWGG 2 cut(s) 331, 493
TaaI ACNGT 3 cut(s) 199, 346, 364
TaiI ACGT 1 cut(s) 575
TaqI TCGA 4 cut(s) 54, 63, 69, 743
TasI AATT 1 cut(s) 751
TatI WGTACW 1 cut(s) 669
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 1 cut(s) 415
TscAI CASTG 4 cut(s) 349, 361, 367, 517
TseFI GTSAC 2 cut(s) 193, 358
TseI GCWGC 1 cut(s) 234
Tsp45I GTSAC 2 cut(s) 193, 358
TspDTI ATGAA 3 cut(s) 17, 121, 532
TspGWI ACGGA 2 cut(s) 35, 110
TspMI CCCGGG 1 cut(s) 91
TspRI CASTG 4 cut(s) 349, 361, 367, 517
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 2 cut(s) 497, 566
XceI RCATGY 1 cut(s) 296
XmaI CCCGGG 1 cut(s) 91
XmiI GTMKAC 2 cut(s) 63, 144
XmnI GAANNNNTTC 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.