MD16G1181200.v1.1

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014400
Physical Location & Seq
Reverse (-)
15553958 .. 15555528
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1181200.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGCTTCCGGAGGGTCAAAATCTGCAGCATCAATTCTCCTAGTTGTTAATCTTATTCTCTATTTCATTGTCGTTGTCATTGCTTCTTGGGCTGTCAACCATGGAATCCAAAGATCTCGCGAAACTGCATCTGTTCTGTCAATTCCAGCTCGGATATTTCCAATATATTTTCCATTTGGGAATTTGGCAACTGGGTTTTTTGTGATCTTCTCCCTCATTGCTGGAGTTGTTGGATTTGGGACCTCACTCACCGGACTCCACAACGTTTTTCAATGGAACAGTCCCAGCTTGCACACAGCTGCTGCCTCTTCTCTTGCTACTTGGTCACTCACCCTTCTTGCCATGGGATTGGCTTGTAAAGAGATTGGACTTGGTTGGACAGATGCCAACATGCGTACTTTGGAGGTCATAACAATAATTGTGAGTGCAACACAAATGTTTTCCACGTGTGTCATTCATGCTGGGGTTGAAGATGCTATTGCTCAAGAAAGAGGACGCAACGAAAGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

171

Amino Acids

18.13

Weight (kDa)

7.88

Isoelectric Point (pI)

27.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 155 2.8e-58 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18970
fragaria_vesca FvH4_4g14182
malus_domestica MD13G1180300.v1.1 MD16G1181200.v1.1
prunus_persica Prupe.1G148600_v2.0.a1
pyrus_communis pycom13g15560 pycom16g15200
rosa_chinensis RchiOBHm_Chr4g0419021
rosa_laevigata RLG00000007832
rosa_multiflora Rmu_co8230677.1_g000001 Rmu_sc0005646.1_g000002
rosa_roxburghii Rroxscaffold_5G00362080
rosa_rugosa Rorug04G0156500
rosa_samantha Rh4AG218000 Rh4BG212200 Rh4DG216600
rosa_wichuraiana Rw4G018640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 8
AclI AACGTT 1 cut(s) 264
AcsI RAATTY 1 cut(s) 181
AcvI CACGTG 1 cut(s) 447
AfaI GTAC 1 cut(s) 397
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 2 cut(s) 272, 470
AluBI AGCT 3 cut(s) 149, 288, 299
AluI AGCT 3 cut(s) 149, 288, 299
AlwNI CAGNNNCTG 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 8
ApeKI GCWGC 3 cut(s) 26, 299, 302
ApoI RAATTY 1 cut(s) 181
AspS9I GGNCC 1 cut(s) 240
AsuHPI GGTGA 2 cut(s) 241, 322
AvaII GGWCC 1 cut(s) 240
BbrPI CACGTG 1 cut(s) 447
BbvI GCAGC 3 cut(s) 38, 286, 289
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 24
BglII AGATCT 1 cut(s) 113
BisI GCNGC 3 cut(s) 27, 300, 303
BlsI GCNGC 3 cut(s) 28, 301, 304
Bme18I GGWCC 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 201
BmsI GCATC 4 cut(s) 38, 137, 373, 463
BmuI ACTGGG 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 243
BpuEI CTTGAG 1 cut(s) 468
BsaAI YACGTR 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 100, 342
BsaWI WCCGGW 2 cut(s) 8, 251
Bse1I ACTGG 1 cut(s) 196
Bse3DI GCAATG 2 cut(s) 78, 216
BseAI TCCGGA 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 100, 342
BseMI GCAATG 2 cut(s) 78, 216
BseNI ACTGG 1 cut(s) 196
BseXI GCAGC 3 cut(s) 38, 286, 289
BseYI CCCAGC 2 cut(s) 284, 461
Bsh1236I CGCG 1 cut(s) 120
BsiSI CCGG 2 cut(s) 9, 252
BslFI GGGAC 2 cut(s) 253, 267
BsmFI GGGAC 2 cut(s) 253, 267
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 2 cut(s) 113, 204
Bsp19I CCATGG 2 cut(s) 100, 342
Bsp68I TCGCGA 1 cut(s) 120
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 28
BsrDI GCAATG 2 cut(s) 78, 216
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 100, 342
BssMI GATC 2 cut(s) 113, 204
BssT1I CCWWGG 2 cut(s) 100, 342
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 447
BstC8I GCNNGC 1 cut(s) 290
BstDSI CCRYGG 2 cut(s) 100, 342
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 2 cut(s) 116, 207
BstMBI GATC 2 cut(s) 113, 204
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 394
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 3 cut(s) 38, 286, 289
BstX2I RGATCY 1 cut(s) 113
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 113
BtgI CCRYGG 2 cut(s) 100, 342
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 290
CaiI CAGNNNCTG 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 240
CseI GACGC 1 cut(s) 504
Csp6I GTAC 1 cut(s) 396
CviAII CATG 4 cut(s) 101, 343, 391, 458
CviJI RGCY 6 cut(s) 5, 92, 149, 288, 299, 353
CviKI_1 RGCY 6 cut(s) 5, 92, 149, 288, 299, 353
CviQI GTAC 1 cut(s) 396
DpnI GATC 2 cut(s) 115, 206
DpnII GATC 2 cut(s) 113, 204
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Eco130I CCWWGG 2 cut(s) 100, 342
Eco47I GGWCC 1 cut(s) 240
Eco72I CACGTG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 240
EcoT14I CCWWGG 2 cut(s) 100, 342
ErhI CCWWGG 2 cut(s) 100, 342
FaeI CATG 4 cut(s) 104, 346, 394, 461
FaiI YATR 6 cut(s) 102, 166, 344, 392, 410, 459
FaqI GGGAC 2 cut(s) 253, 267
FatI CATG 4 cut(s) 100, 342, 390, 457
Fnu4HI GCNGC 3 cut(s) 27, 300, 303
Fsp4HI GCNGC 3 cut(s) 27, 300, 303
FspBI CTAG 1 cut(s) 41
GluI GCNGC 3 cut(s) 27, 300, 303
GsaI CCCAGC 2 cut(s) 288, 465
GsuI CTGGAG 1 cut(s) 243
HapII CCGG 2 cut(s) 9, 252
HgaI GACGC 1 cut(s) 504
Hin1II CATG 4 cut(s) 104, 346, 394, 461
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 2 cut(s) 105, 255
HpaII CCGG 2 cut(s) 9, 252
HphI GGTGA 2 cut(s) 241, 322
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 3 cut(s) 9, 119, 485
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 2 cut(s) 264, 446
HpyCH4V TGCA 4 cut(s) 26, 128, 292, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpySE526I ACGT 2 cut(s) 264, 446
Hsp92II CATG 4 cut(s) 104, 346, 394, 461
Kpn2I TCCGGA 1 cut(s) 8
Kzo9I GATC 2 cut(s) 113, 204
LpnPI CCDG 7 cut(s) 22, 159, 177, 207, 265, 298, 447
Lsp1109I GCAGC 3 cut(s) 38, 286, 289
LweI GCATC 4 cut(s) 38, 137, 373, 463
MaeI CTAG 1 cut(s) 41
MaeII ACGT 2 cut(s) 264, 446
MaeIII GTNAC 1 cut(s) 324
MalI GATC 2 cut(s) 115, 206
MboI GATC 2 cut(s) 113, 204
MboII GAAGA 3 cut(s) 199, 300, 482
MflI RGATCY 1 cut(s) 113
MluCI AATT 4 cut(s) 33, 141, 181, 417
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 2 cut(s) 211, 356
MnlI CCTC 6 cut(s) 5, 224, 253, 316, 397, 485
MroI TCCGGA 1 cut(s) 8
MseI TTAA 2 cut(s) 48, 511
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 9, 252
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 89
NcoI CCATGG 2 cut(s) 100, 342
NdeII GATC 2 cut(s) 113, 204
NlaIII CATG 4 cut(s) 104, 346, 394, 461
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 324
NruI TCGCGA 1 cut(s) 120
NspI RCATGY 1 cut(s) 394
PfeI GAWTC 1 cut(s) 105
PkrI GCNGC 3 cut(s) 28, 301, 304
PleI GAGTC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 447
PmlI CACGTG 1 cut(s) 447
PpsI GAGTC 1 cut(s) 249
Ppu21I YACGTR 1 cut(s) 447
PpuMI RGGWCCY 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 240
PspCI CACGTG 1 cut(s) 447
PspFI CCCAGC 2 cut(s) 284, 461
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 240
PspPPI RGGWCCY 1 cut(s) 240
PstI CTGCAG 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 299
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
SaqAI TTAA 2 cut(s) 48, 511
SatI GCNGC 3 cut(s) 27, 300, 303
Sau3AI GATC 2 cut(s) 113, 204
Sau96I GGNCC 1 cut(s) 240
SchI GAGTC 1 cut(s) 249
SetI ASST 7 cut(s) 151, 245, 267, 290, 301, 408, 449
SfaNI GCATC 4 cut(s) 38, 137, 373, 463
SfcI CTRYAG 1 cut(s) 24
SinI GGWCC 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
Sse9I AATT 4 cut(s) 33, 141, 181, 417
SspMI CTAG 1 cut(s) 41
StyI CCWWGG 2 cut(s) 100, 342
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 2 cut(s) 267, 449
TasI AATT 4 cut(s) 33, 141, 181, 417
TfiI GAWTC 1 cut(s) 105
Tru1I TTAA 2 cut(s) 48, 511
Tru9I TTAA 2 cut(s) 48, 511
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 3 cut(s) 26, 299, 302
Tsp45I GTSAC 1 cut(s) 324
TspDTI ATGAA 2 cut(s) 55, 446
VpaK11BI GGWCC 1 cut(s) 240
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 394
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.