Rh4AG218000

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014400
Physical Location & Seq
Forward (+)
51554773 .. 51555211
439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG218000.1

Sequence Viewer

Length: 354 bp
ATGGTTCCTGGAGGAGGGGCAAAATCAGCAGCCTTTGTTCTCTTACTTGTTAATCTCGTTATCTTCTTCATTGTGACTGTCATAGCTGCATGGGCAATGAACCATGGGATCCAAAGGTCTCGTGAAGCAGCATCTGTTTTGTCCATACCATTGCGCATTTTTCCAATATACTTCCCAATGGGGAATATGGCAACCGGTTTCTTTGTGATCTTCTCCCTCATTGCCGGTGTTGTTGGAATTGCCACCTCCCTTACCGGACTTCATAACGTTTTTCAATGGGATGGCCCCAGTTTACACACAGCTGCTGCCTCTTCCCTTGTATCATGGTCCCTCACTCTTCTTGCCATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

117

Amino Acids

12.33

Weight (kDa)

9.98

Isoelectric Point (pI)

20.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 16 - 117 1.7e-39 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18970
fragaria_vesca FvH4_4g14182
malus_domestica MD13G1180300.v1.1 MD16G1181200.v1.1
prunus_persica Prupe.1G148600_v2.0.a1
pyrus_communis pycom13g15560 pycom16g15200
rosa_chinensis RchiOBHm_Chr4g0419021
rosa_laevigata RLG00000007832
rosa_multiflora Rmu_co8230677.1_g000001 Rmu_sc0005646.1_g000002
rosa_roxburghii Rroxscaffold_5G00362080
rosa_rugosa Rorug04G0156500
rosa_samantha Rh4AG218000 Rh4BG212200 Rh4DG216600
rosa_wichuraiana Rw4G018640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 155
AclI AACGTT 1 cut(s) 267
AclWI GGATC 2 cut(s) 103, 116
AfiI CCNNNNNNNGG 1 cut(s) 14
AgeI ACCGGT 1 cut(s) 194
AgsI TTSAA 1 cut(s) 275
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 2 cut(s) 86, 302
AluI AGCT 2 cut(s) 86, 302
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 2 cut(s) 103, 116
AlwNI CAGNNNCTG 2 cut(s) 134, 305
AoxI GGCC 1 cut(s) 283
ApeKI GCWGC 5 cut(s) 29, 86, 128, 302, 305
AsiGI ACCGGT 1 cut(s) 194
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 284, 327
AvaII GGWCC 1 cut(s) 327
BamHI GGATCC 1 cut(s) 108
BauI CACGAG 1 cut(s) 120
BbvI GCAGC 5 cut(s) 41, 73, 140, 289, 292
BccI CCATC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 123
BisI GCNGC 5 cut(s) 30, 87, 129, 303, 306
BlsI GCNGC 5 cut(s) 31, 88, 130, 304, 307
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 2 cut(s) 284, 327
BmiI GGNNCC 4 cut(s) 6, 110, 286, 329
BmrFI CCNGG 1 cut(s) 9
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 1 cut(s) 140
BmuI ACTGGG 1 cut(s) 282
BpmI CTGGAG 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 103, 345
BsaWI WCCGGW 2 cut(s) 194, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse118I RCCGGY 2 cut(s) 194, 224
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 3 cut(s) 102, 149, 219
BseBI CCWGG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 103, 345
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 14
BseMI GCAATG 3 cut(s) 102, 149, 219
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 5 cut(s) 41, 73, 140, 289, 292
BshFI GGCC 1 cut(s) 285
BshTI ACCGGT 1 cut(s) 194
BsiSI CCGG 3 cut(s) 195, 225, 255
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 1 cut(s) 285
Bso31I GGTCTC 1 cut(s) 123
Bsp143I GATC 2 cut(s) 108, 207
Bsp19I CCATGG 2 cut(s) 103, 345
BspANI GGCC 1 cut(s) 285
BspLI GGNNCC 4 cut(s) 6, 110, 286, 329
BspPI GGATC 2 cut(s) 103, 116
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 3 cut(s) 102, 149, 219
BsrFI RCCGGY 2 cut(s) 194, 224
BsrI ACTGG 1 cut(s) 288
BssAI RCCGGY 2 cut(s) 194, 224
BssECI CCNNGG 2 cut(s) 103, 345
BssMI GATC 2 cut(s) 108, 207
BssSI CACGAG 1 cut(s) 120
BssT1I CCWWGG 2 cut(s) 103, 345
Bst2BI CACGAG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 2 cut(s) 316, 342
BstDSI CCRYGG 2 cut(s) 103, 345
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 2 cut(s) 111, 210
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 108, 207
BstMWI GCNNNNNNNGC 2 cut(s) 26, 92
BstNI CCWGG 1 cut(s) 9
BstSCI CCNGG 1 cut(s) 7
BstV1I GCAGC 5 cut(s) 41, 73, 140, 289, 292
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 1 cut(s) 285
BtgI CCRYGG 2 cut(s) 103, 345
BtsCI GGATG 1 cut(s) 286
CaiI CAGNNNCTG 2 cut(s) 134, 305
CfoI GCGC 1 cut(s) 156
Cfr10I RCCGGY 2 cut(s) 194, 224
Cfr13I GGNCC 2 cut(s) 284, 327
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 4 cut(s) 90, 104, 324, 346
CviJI RGCY 4 cut(s) 32, 86, 285, 302
CviKI_1 RGCY 4 cut(s) 32, 86, 285, 302
DpnI GATC 2 cut(s) 110, 209
DpnII GATC 2 cut(s) 108, 207
Eam1104I CTCTTC 2 cut(s) 316, 342
EarI CTCTTC 2 cut(s) 316, 342
Eco130I CCWWGG 2 cut(s) 103, 345
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 7
EcoT14I CCWWGG 2 cut(s) 103, 345
ErhI CCWWGG 2 cut(s) 103, 345
FaeI CATG 4 cut(s) 93, 107, 327, 349
FaiI YATR 9 cut(s) 83, 91, 105, 146, 169, 188, 264, 325, 347
FaqI GGGAC 1 cut(s) 313
FatI CATG 4 cut(s) 89, 103, 323, 345
Fnu4HI GCNGC 5 cut(s) 30, 87, 129, 303, 306
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 5 cut(s) 30, 87, 129, 303, 306
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 1 cut(s) 155
GluI GCNGC 5 cut(s) 30, 87, 129, 303, 306
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 1 cut(s) 285
HapII CCGG 3 cut(s) 195, 225, 255
HhaI GCGC 1 cut(s) 156
Hin1II CATG 4 cut(s) 93, 107, 327, 349
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HpaII CCGG 3 cut(s) 195, 225, 255
Hpy166II GTNNAC 1 cut(s) 293
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 1 cut(s) 89
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 92
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 4 cut(s) 93, 107, 327, 349
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 108, 207
LpnPI CCDG 5 cut(s) 21, 208, 238, 268, 301
Lsp1109I GCAGC 5 cut(s) 41, 73, 140, 289, 292
LweI GCATC 1 cut(s) 140
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 110, 209
MboI GATC 2 cut(s) 108, 207
MboII GAAGA 5 cut(s) 55, 58, 202, 303, 329
MflI RGATCY 1 cut(s) 108
MluCI AATT 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 6 cut(s) 5, 8, 227, 256, 319, 341
MseI TTAA 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 3 cut(s) 195, 225, 255
MspR9I CCNGG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 2 cut(s) 26, 92
NcoI CCATGG 2 cut(s) 103, 345
NdeII GATC 2 cut(s) 108, 207
NlaIII CATG 4 cut(s) 93, 107, 327, 349
NlaIV GGNNCC 4 cut(s) 6, 110, 286, 329
NmuCI GTSAC 1 cut(s) 73
NsbI TGCGCA 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 7
PinAI ACCGGT 1 cut(s) 194
PkrI GCNGC 5 cut(s) 31, 88, 130, 304, 307
Psp1406I AACGTT 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 4 cut(s) 6, 110, 286, 329
PspPI GGNCC 2 cut(s) 284, 327
PstNI CAGNNNCTG 2 cut(s) 134, 305
PsuI RGATCY 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 302
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 5 cut(s) 30, 87, 129, 303, 306
Sau3AI GATC 2 cut(s) 108, 207
Sau96I GGNCC 2 cut(s) 284, 327
ScrFI CCNGG 1 cut(s) 9
SetI ASST 5 cut(s) 88, 119, 248, 270, 304
SfaNI GCATC 1 cut(s) 140
SinI GGWCC 1 cut(s) 327
Sse9I AATT 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 7
StyI CCWWGG 2 cut(s) 103, 345
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 270
TasI AATT 1 cut(s) 237
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 5 cut(s) 29, 86, 128, 302, 305
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 3 cut(s) 58, 113, 251
VpaK11BI GGWCC 1 cut(s) 327
XagI CCTNNNNNAGG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.