Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009789

Physical Location & Seq

Genomic Coordinates Reverse (-)

15556005 .. 15560438

Gene Structure 4434 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1181300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

ATGAAGGTTACAAAGAGCTCAGCAACTGCCAGTCCTATGACAGCAAAGATGTGGGGTTCTCTTGTGTTGAAACAGAACAAGAAAGGACCAACTCGGAGCTCTACTAAATTTAGAGTGCTCGCCACCAAGTCCGAAGGTTATACCATAAACAGGCTAGAGGGTTTACTAAACCTGGACCTCACTCCATTCACTGACAAGATTATTGCTGAGTACATTTGGATTGGAGGGTCAGGGATTGATGTTCGTAGCAAGTCAAGGACATTTTCAAAGCCTGTTGAAGATCCTTCTGAGCTTCCCAAGTGGAATTATGATGGATCAAGTACTGGACAAGCTCCTGGTGAAGACAGTGAAGTAATCTTATACCCTCAGGCGATATTTAAGGACCCTTTCCGTGGTGGCAACAATATTCTGGTAATTTGCGATGCATACACACCGCAAGGCGAGCCTATCCCAACAAACAAGCGCGCCAGGGCTGCTGAGATTTTCAAGAACAAGAAGGTTATAGACGAAGTCCCATGGTATGGGATTGAGCAAGAGTACACATTACTCCAAACTGATGTGAAATGGCCTTTGGGTTGGCCAGTTGGAGGTTATCCTGGTCCTCAGGGTCCTTACTACTGTGGTGCTGGTGCAGACAAGTCATTCGGACGTGACATATCAGATGCTCACTACAAGGCTTGCCTATATGCCGGAATTAACATCAGTGGCACCAATGGGGAGGTTATGCCTGGCCAGTGGGAGTATCAAGTTGGTCCGAGTGTGGGAATTGAAGCAGGAGATCATATCTGGGCTTCAAGATACATTCTTGAGAGAATCACTGAACAAGCTGGTGTTGTTCTCACACTTGATCCAAAACCTATAGAGGGTGACTGGAACGGTGCAGGATGCCACACCAATTACAGTACAAAGAGCATGAGAGAAGACGGAGGCTTTGAAGTTATTAAGAAGGCAATTTTGAATCTGTCACTTCGCCATGGTGAGCACATTAGTGCCTACGGAGAAGGAAATGAGAGAAGGTTGACAGGAAAGCATGAAACAGCCAGCATTAACACATTTTCTTGGGGAGTGGCTAATCGTGGTTGCTCAATCCGTGTTGGTCGTGAAACTGAGAAGCAAGGAAAAGGTTATCTGGAGGATCGTCGTCCAGCTTCAAACATGGACCCTTACATTGTGACCTCACTACTGGCAGAAACTACATTGTTGTGGGAGCCAACCCTTGAGGCTGAAGCTCTTGCAGCTCAAAAGCTTTCATTGAATGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004356 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006536 GO:0006541 GO:0006807 GO:0006950 GO:0006970 GO:0007568 GO:0008150 GO:0008152 GO:0009064 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016211 GO:0016874 GO:0016879 GO:0016880 GO:0019676 GO:0019740 GO:0019752 GO:0022626 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032502 GO:0032991 GO:0034357 GO:0042221 GO:0042651 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0055035 GO:0071704 GO:1901564 GO:1901605 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

421

Amino Acids

46.22

Weight (kDa)

6.37

Isoelectric Point (pI)

41.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N	PF03951	80 - 145	8.3e-09	Glutamine synthetase, beta-Grasp domain
Gln-synt_C	PF00120	175 - 302	4.5e-10	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009789)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35630 AT5G35630 AT5G35630
fragaria_vesca	FvH4_4g14190 FvH4_4g14190
malus_domestica	MD13G1180400.v1.1 MD16G1181300.v1.1
prunus_persica	Prupe.1G148700_v2.0.a1
pyrus_communis	pycom13g15570 pycom16g15210
rosa_chinensis	RchiOBHm_Chr4g0419011
rosa_laevigata	RLG00000007833
rosa_multiflora	Rmu_sc0005646.1_g000003
rosa_roxburghii	Rroxscaffold_5G00362070
rosa_rugosa	Rorug04G0156200 Rorug04G0156300
rosa_samantha	Rh4AG217900 Rh4BG212300 Rh4CG229900 Rh4DG216500
rosa_wichuraiana	Rw4G018630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	707
AccB7I	CCANNNNNTGG	1 cut(s)	521
AccII	CGCG	1 cut(s)	465
AciI	CCGC	1 cut(s)	434
AclWI	GGATC	4 cut(s)	275, 322, 842, 1143
AcoI	YGGCCR	2 cut(s)	578, 730
AcsI	RAATTY	1 cut(s)	107
AcuI	CTGAAG	1 cut(s)	1245
AfaI	GTAC	4 cut(s)	212, 322, 539, 904
AfiI	CCNNNNNNNGG	6 cut(s)	150, 392, 521, 587, 761, 863
AjiI	CACGTC	1 cut(s)	650
AjnI	CCWGG	5 cut(s)	171, 334, 467, 595, 727
AjuI	GAANNNNNNNTTGG	2 cut(s)	554, 586
AleI	CACNNNNGTG	1 cut(s)	987
AluBI	AGCT	9 cut(s)	18, 99, 292, 332, 827, 1148, 1229, 1238, 1246
AluI	AGCT	9 cut(s)	18, 99, 292, 332, 827, 1148, 1229, 1238, 1246
Alw21I	GWGCWC	4 cut(s)	20, 101, 120, 984
AlwI	GGATC	4 cut(s)	275, 322, 842, 1143
AlwNI	CAGNNNCTG	1 cut(s)	26
AoxI	GGCC	3 cut(s)	566, 578, 730
ApeKI	GCWGC	2 cut(s)	473, 1235
ApoI	RAATTY	1 cut(s)	107
ArsI	GACNNNNNNTTYG	4 cut(s)	626, 658, 914, 946
AspLEI	GCGC	2 cut(s)	465, 467
AspS9I	GGNCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
AsuHPI	GGTGA	3 cut(s)	350, 878, 989
AvaII	GGWCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
AxyI	CCTNAGG	2 cut(s)	366, 603
BalI	TGGCCA	2 cut(s)	580, 732
BanI	GGYRCC	1 cut(s)	707
BanII	GRGCYC	2 cut(s)	20, 101
BbsI	GAAGAC	2 cut(s)	348, 927
Bbv12I	GWGCWC	4 cut(s)	20, 101, 120, 984
BbvI	GCAGC	2 cut(s)	460, 1247
BccI	CCATC	1 cut(s)	305
BciT130I	CCWGG	5 cut(s)	173, 336, 469, 597, 729
BfaI	CTAG	1 cut(s)	155
BfmI	CTRYAG	1 cut(s)	858
BisI	GCNGC	2 cut(s)	474, 1236
BlpI	GCTNAGC	1 cut(s)	19
BlsI	GCNGC	2 cut(s)	475, 1237
BmcAI	AGTACT	1 cut(s)	322
Bme1390I	CCNGG	5 cut(s)	173, 336, 469, 597, 729
Bme18I	GGWCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
BmgBI	CACGTC	1 cut(s)	650
BmgT120I	GGNCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
BmiI	GGNNCC	5 cut(s)	384, 609, 709, 1161, 1209
BmrFI	CCNGG	5 cut(s)	173, 336, 469, 597, 729
BmsI	GCATC	3 cut(s)	412, 652, 875
BpiI	GAAGAC	2 cut(s)	348, 927
BpmI	CTGGAG	1 cut(s)	1151
Bpu1102I	GCTNAGC	1 cut(s)	19
BpuEI	CTTGAG	2 cut(s)	827, 1238
BsaJI	CCNNGG	4 cut(s)	391, 468, 515, 973
Bsc4I	CCNNNNNNNGG	6 cut(s)	150, 392, 521, 587, 761, 863
Bse1I	ACTGG	6 cut(s)	30, 328, 581, 733, 875, 1188
Bse21I	CCTNAGG	2 cut(s)	366, 603
BseBI	CCWGG	5 cut(s)	173, 336, 469, 597, 729
BseDI	CCNNGG	4 cut(s)	391, 468, 515, 973
BseGI	GGATG	1 cut(s)	890
BseLI	CCNNNNNNNGG	6 cut(s)	150, 392, 521, 587, 761, 863
BseMII	CTCAG	7 cut(s)	33, 198, 279, 380, 468, 617, 1098
BseNI	ACTGG	6 cut(s)	30, 328, 581, 733, 875, 1188
BsePI	GCGCGC	1 cut(s)	463
BseXI	GCAGC	2 cut(s)	460, 1247
BsgI	GTGCAG	2 cut(s)	651, 900
Bsh1236I	CGCG	1 cut(s)	465
BshFI	GGCC	3 cut(s)	568, 580, 732
BshNI	GGYRCC	1 cut(s)	707
BsiHKAI	GWGCWC	4 cut(s)	20, 101, 120, 984
BsiSI	CCGG	1 cut(s)	690
BslFI	GGGAC	1 cut(s)	497
BslI	CCNNNNNNNGG	6 cut(s)	150, 392, 521, 587, 761, 863
BsmFI	GGGAC	1 cut(s)	497
BsnI	GGCC	3 cut(s)	568, 580, 732
Bsp1286I	GDGCHC	4 cut(s)	20, 101, 120, 984
Bsp143I	GATC	5 cut(s)	280, 314, 778, 847, 1135
Bsp1720I	GCTNAGC	1 cut(s)	19
Bsp19I	CCATGG	2 cut(s)	515, 973
BspACI	CCGC	1 cut(s)	434
BspANI	GGCC	3 cut(s)	568, 580, 732
BspCNI	CTCAG	7 cut(s)	32, 199, 280, 379, 469, 616, 1099
BspFNI	CGCG	1 cut(s)	465
BspLI	GGNNCC	5 cut(s)	384, 609, 709, 1161, 1209
BspPI	GGATC	4 cut(s)	275, 322, 842, 1143
BspT107I	GGYRCC	1 cut(s)	707
BsrI	ACTGG	6 cut(s)	30, 328, 581, 733, 875, 1188
BssECI	CCNNGG	4 cut(s)	391, 468, 515, 973
BssHII	GCGCGC	1 cut(s)	463
BssMI	GATC	5 cut(s)	280, 314, 778, 847, 1135
BssT1I	CCWWGG	2 cut(s)	515, 973
Bst2UI	CCWGG	5 cut(s)	173, 336, 469, 597, 729
Bst4CI	ACNGT	4 cut(s)	347, 620, 878, 902
BstC8I	GCNNGC	5 cut(s)	120, 443, 465, 679, 1042
BstDEI	CTNAG	7 cut(s)	19, 207, 288, 366, 477, 603, 1107
BstDSI	CCRYGG	3 cut(s)	391, 515, 973
BstF5I	GGATG	1 cut(s)	890
BstFNI	CGCG	1 cut(s)	465
BstHHI	GCGC	2 cut(s)	465, 467
BstKTI	GATC	5 cut(s)	283, 317, 781, 850, 1138
BstMBI	GATC	5 cut(s)	280, 314, 778, 847, 1135
BstMWI	GCNNNNNNNGC	3 cut(s)	442, 473, 1235
BstNI	CCWGG	5 cut(s)	173, 336, 469, 597, 729
BstSCI	CCNGG	5 cut(s)	171, 334, 467, 595, 727
BstSFI	CTRYAG	1 cut(s)	858
BstUI	CGCG	1 cut(s)	465
BstV1I	GCAGC	2 cut(s)	460, 1247
BstV2I	GAAGAC	2 cut(s)	348, 927
BstX2I	RGATCY	1 cut(s)	280
BstYI	RGATCY	1 cut(s)	280
Bsu36I	CCTNAGG	2 cut(s)	366, 603
BsuRI	GGCC	3 cut(s)	568, 580, 732
BtgI	CCRYGG	3 cut(s)	391, 515, 973
BtgZI	GCGATG	1 cut(s)	435
BtrI	CACGTC	1 cut(s)	650
BtsCI	GGATG	1 cut(s)	890
BtsIMutI	CAGTG	5 cut(s)	189, 352, 709, 740, 816
Cac8I	GCNNGC	5 cut(s)	120, 443, 465, 679, 1042
CaiI	CAGNNNCTG	1 cut(s)	26
CfoI	GCGC	2 cut(s)	465, 467
Cfr13I	GGNCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
Csp6I	GTAC	4 cut(s)	211, 321, 538, 903
CviAII	CATG	5 cut(s)	516, 913, 974, 1031, 1156
CviQI	GTAC	4 cut(s)	211, 321, 538, 903
DdeI	CTNAG	7 cut(s)	19, 207, 288, 366, 477, 603, 1107
DpnI	GATC	5 cut(s)	282, 316, 780, 849, 1137
DpnII	GATC	5 cut(s)	280, 314, 778, 847, 1135
EaeI	YGGCCR	2 cut(s)	578, 730
Ecl136II	GAGCTC	2 cut(s)	18, 99
Eco130I	CCWWGG	2 cut(s)	515, 973
Eco24I	GRGCYC	2 cut(s)	20, 101
Eco47I	GGWCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
Eco53kI	GAGCTC	2 cut(s)	18, 99
Eco57I	CTGAAG	1 cut(s)	1245
Eco81I	CCTNAGG	2 cut(s)	366, 603
EcoICRI	GAGCTC	2 cut(s)	18, 99
EcoO109I	RGGNCCY	2 cut(s)	382, 608
EcoRII	CCWGG	5 cut(s)	171, 334, 467, 595, 727
EcoT14I	CCWWGG	2 cut(s)	515, 973
EcoT22I	ATGCAT	1 cut(s)	427
EcoT38I	GRGCYC	2 cut(s)	20, 101
ErhI	CCWWGG	2 cut(s)	515, 973
FaeI	CATG	5 cut(s)	519, 916, 977, 1034, 1159
FaqI	GGGAC	1 cut(s)	497
FatI	CATG	5 cut(s)	515, 912, 973, 1030, 1155
Fnu4HI	GCNGC	2 cut(s)	474, 1236
FokI	GGATG	1 cut(s)	897
FriOI	GRGCYC	2 cut(s)	20, 101
Fsp4HI	GCNGC	2 cut(s)	474, 1236
FspBI	CTAG	1 cut(s)	155
GlaI	GCGC	2 cut(s)	464, 466
GluI	GCNGC	2 cut(s)	474, 1236
GsuI	CTGGAG	1 cut(s)	1151
HaeIII	GGCC	3 cut(s)	568, 580, 732
HapII	CCGG	1 cut(s)	690
HhaI	GCGC	2 cut(s)	465, 467
Hin1II	CATG	5 cut(s)	519, 916, 977, 1034, 1159
Hin6I	GCGC	2 cut(s)	463, 465
HinP1I	GCGC	2 cut(s)	463, 465
HincII	GTYRAC	1 cut(s)	1020
HindII	GTYRAC	1 cut(s)	1020
HindIII	AAGCTT	1 cut(s)	1244
HinfI	GANTC	2 cut(s)	813, 958
HpaII	CCGG	1 cut(s)	690
HphI	GGTGA	3 cut(s)	350, 878, 989
Hpy166II	GTNNAC	3 cut(s)	164, 540, 1020
Hpy188I	TCNGA	7 cut(s)	96, 133, 289, 647, 661, 756, 1262
Hpy188III	TCNNGA	5 cut(s)	487, 795, 806, 1100, 1130
Hpy8I	GTNNAC	3 cut(s)	164, 540, 1020
Hpy99I	CGWCG	1 cut(s)	1143
HpyAV	CCTTC	6 cut(s)	128, 294, 490, 940, 995, 1008
HpyCH4III	ACNGT	4 cut(s)	347, 620, 878, 902
HpyCH4IV	ACGT	1 cut(s)	649
HpyCH4V	TGCA	4 cut(s)	425, 632, 881, 1235
HpyF10VI	GCNNNNNNNGC	3 cut(s)	442, 473, 1235
HpyF3I	CTNAG	7 cut(s)	19, 207, 288, 366, 477, 603, 1107
HpySE526I	ACGT	1 cut(s)	649
Hsp92II	CATG	5 cut(s)	519, 916, 977, 1034, 1159
HspAI	GCGC	2 cut(s)	463, 465
Kzo9I	GATC	5 cut(s)	280, 314, 778, 847, 1135
LmnI	GCTCC	3 cut(s)	96, 337, 1207
Lsp1109I	GCAGC	2 cut(s)	460, 1247
LweI	GCATC	3 cut(s)	412, 652, 875
MaeI	CTAG	1 cut(s)	155
MaeII	ACGT	1 cut(s)	649
MaeIII	GTNAC	5 cut(s)	7, 650, 866, 963, 1171
MalI	GATC	5 cut(s)	282, 316, 780, 849, 1137
MboI	GATC	5 cut(s)	280, 314, 778, 847, 1135
MboII	GAAGA	3 cut(s)	290, 353, 932
MflI	RGATCY	1 cut(s)	280
MhlI	GDGCHC	4 cut(s)	20, 101, 120, 984
MlsI	TGGCCA	2 cut(s)	580, 732
MluCI	AATT	7 cut(s)	107, 304, 414, 693, 765, 895, 951
MluNI	TGGCCA	2 cut(s)	580, 732
MmeI	TCCRAC	1 cut(s)	565
Mox20I	TGGCCA	2 cut(s)	580, 732
Mph1103I	ATGCAT	1 cut(s)	427
MscI	TGGCCA	2 cut(s)	580, 732
MseI	TTAA	4 cut(s)	378, 696, 942, 1047
MslI	CAYNNNNRTG	2 cut(s)	987, 1201
Msp20I	TGGCCA	2 cut(s)	580, 732
MspI	CCGG	1 cut(s)	690
MspR9I	CCNGG	5 cut(s)	173, 336, 469, 597, 729
MvaI	CCWGG	5 cut(s)	173, 336, 469, 597, 729
MvnI	CGCG	1 cut(s)	465
MwoI	GCNNNNNNNGC	3 cut(s)	442, 473, 1235
NcoI	CCATGG	2 cut(s)	515, 973
NdeII	GATC	5 cut(s)	280, 314, 778, 847, 1135
NlaIII	CATG	5 cut(s)	519, 916, 977, 1034, 1159
NlaIV	GGNNCC	5 cut(s)	384, 609, 709, 1161, 1209
NmuCI	GTSAC	4 cut(s)	650, 866, 963, 1171
NsiI	ATGCAT	1 cut(s)	427
OliI	CACNNNNGTG	1 cut(s)	987
PauI	GCGCGC	1 cut(s)	463
PfeI	GAWTC	2 cut(s)	813, 958
PflFI	GACNNNGTC	1 cut(s)	509
PflMI	CCANNNNNTGG	1 cut(s)	521
PkrI	GCNGC	2 cut(s)	475, 1237
PpuMI	RGGWCCY	2 cut(s)	382, 608
Psp124BI	GAGCTC	2 cut(s)	20, 101
Psp5II	RGGWCCY	2 cut(s)	382, 608
Psp6I	CCWGG	5 cut(s)	171, 334, 467, 595, 727
PspGI	CCWGG	5 cut(s)	171, 334, 467, 595, 727
PspN4I	GGNNCC	5 cut(s)	384, 609, 709, 1161, 1209
PspPI	GGNCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
PspPPI	RGGWCCY	2 cut(s)	382, 608
PstNI	CAGNNNCTG	1 cut(s)	26
PsuI	RGATCY	1 cut(s)	280
PsyI	GACNNNGTC	1 cut(s)	509
PteI	GCGCGC	1 cut(s)	463
RsaI	GTAC	4 cut(s)	212, 322, 539, 904
RsaNI	GTAC	4 cut(s)	211, 321, 538, 903
RseI	CAYNNNNRTG	2 cut(s)	987, 1201
SacI	GAGCTC	2 cut(s)	20, 101
SaqAI	TTAA	4 cut(s)	378, 696, 942, 1047
SatI	GCNGC	2 cut(s)	474, 1236
Sau3AI	GATC	5 cut(s)	280, 314, 778, 847, 1135
Sau96I	GGNCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
ScaI	AGTACT	1 cut(s)	322
ScrFI	CCNGG	5 cut(s)	173, 336, 469, 597, 729
SduI	GDGCHC	4 cut(s)	20, 101, 120, 984
SfaNI	GCATC	3 cut(s)	412, 652, 875
SfcI	CTRYAG	1 cut(s)	858
SinI	GGWCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
SmiMI	CAYNNNNRTG	2 cut(s)	987, 1201
SmlI	CTYRAG	2 cut(s)	806, 1217
SmoI	CTYRAG	2 cut(s)	806, 1217
Sse9I	AATT	7 cut(s)	107, 304, 414, 693, 765, 895, 951
SsiI	CCGC	1 cut(s)	434
SspI	AATATT	1 cut(s)	406
SspMI	CTAG	1 cut(s)	155
SstI	GAGCTC	2 cut(s)	20, 101
StyD4I	CCNGG	5 cut(s)	171, 334, 467, 595, 727
StyI	CCWWGG	2 cut(s)	515, 973
TaaI	ACNGT	4 cut(s)	347, 620, 878, 902
TaiI	ACGT	1 cut(s)	652
TasI	AATT	7 cut(s)	107, 304, 414, 693, 765, 895, 951
TatI	WGTACW	4 cut(s)	210, 320, 537, 902
TfiI	GAWTC	2 cut(s)	813, 958
Tru1I	TTAA	4 cut(s)	378, 696, 942, 1047
Tru9I	TTAA	4 cut(s)	378, 696, 942, 1047
TscAI	CASTG	5 cut(s)	196, 352, 709, 740, 823
TseFI	GTSAC	4 cut(s)	650, 866, 963, 1171
TseI	GCWGC	2 cut(s)	473, 1235
Tsp45I	GTSAC	4 cut(s)	650, 866, 963, 1171
TspDTI	ATGAA	3 cut(s)	17, 1047, 1239
TspGWI	ACGGA	4 cut(s)	380, 939, 1011, 1079
TspRI	CASTG	5 cut(s)	196, 352, 709, 740, 823
Tth111I	GACNNNGTC	1 cut(s)	509
Van91I	CCANNNNNTGG	1 cut(s)	521
VpaK11BI	GGWCC	7 cut(s)	86, 175, 382, 599, 608, 752, 1159
XapI	RAATTY	1 cut(s)	107
XspI	CTAG	1 cut(s)	155
ZrmI	AGTACT	1 cut(s)	322
Zsp2I	ATGCAT	1 cut(s)	427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.