MD16G1185600.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014604
Physical Location & Seq
Forward (+)
15991079 .. 15992684
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1185600.v1.1.491

Sequence Viewer

Length: 903 bp
ATGAAAGGCTTTTCTACAAATGAAATTTGTGTTTCCTCTTCTCAAACTTCAAACTGCACATCAACCAGTGGCCATTCCAAAGATGACCAAGAAATGGGGTCTAATGCAATGTCAAATGGGGGAAATGTCTTGAGAAATTCAATGTTCTTGTGTTTTCCACTCCAGATAAATCCAAATCTTGTACGCGCGGTTTTGGCTGAGATGGTGGGGACTTTTATCTTGATGTTCTGTGTATGTGGGATCATAGCAAGCACACAGCTGATGAAAGGTGAAGTAGGTCTTCTGGAGTATGCAGCCACAGCAGGTTTAACAGTAGTAGTTGTTATTTTCTCCATAGGGTCTATCTCCGGTGCTCATGTCAACCCTGCTGTCACATTGGCCTTTGCCACCTTCGGTCATTTTCCATGGTCCAGGGTTCCTCTTTACACATTGGCACAAACACTGGGTTCTGTTCTGGCAACATGTATTGGAAGGCTTATCTATGGCATAAAACCAGATCTCCTCACTACCAGACCACTGCAAAGCTGTGCTTCTGCCTTCTGGGTTGAGCTCATTGCAACTTTCATGATTATGTTCCTAGCTGCGTCATTGACACATCAAGCTCAAGCTGTTGGCCATTTGTCTGGATTTGTGGTTGGCATTGCCATCGGACTAGCTGTACTAATCACAGGGCCTATTTCAGGAGGGTCAATGAACCCAGCAAGGTCACTGGGACCTGCAATTGTTTCATGGAATTTCAAAGACATATGGATATATATATGTGGACCAACTGTTGGAGCTGTCGCCGGTGCTCTACTATATCAAATTCTTCATCTTCGATGCCCGCCACCCTGCAGTCCCAGTAACTCTTCCTCTCCAAATATACATCTGCTAGGGCAACCCTTGGCCTATGGAGCAACCTAA

Protein Analysis

301

Amino Acids

31.46

Weight (kDa)

8.05

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 59 - 267 6.8e-52 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014604)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06100 AT3G06100 AT3G06100
fragaria_vesca FvH4_4g14730 FvH4_4g14730
malus_domestica MD16G1185600.v1.1
prunus_persica Prupe.1G152300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0417871
rosa_laevigata RLG00000007891
rosa_multiflora Rmu_sc0004911.1_g000004
rosa_roxburghii Rroxscaffold_5G00361290
rosa_rugosa Rorug04G0150100
rosa_samantha Rh4AG212300 Rh4BG218700 Rh4CG223300 Rh4DG210200
rosa_wichuraiana Rw4G018020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 293, 724
AccB7I CCANNNNNTGG 2 cut(s) 94, 773
AccII CGCG 2 cut(s) 186, 188
AciI CCGC 2 cut(s) 188, 824
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 2 cut(s) 70, 613
AcsI RAATTY 4 cut(s) 24, 136, 733, 804
AfaI GTAC 2 cut(s) 183, 660
AfiI CCNNNNNNNGG 3 cut(s) 94, 680, 773
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 3 cut(s) 51, 141, 739
AjnI CCWGG 1 cut(s) 410
AluBI AGCT 8 cut(s) 259, 525, 550, 581, 602, 608, 656, 779
AluI AGCT 8 cut(s) 259, 525, 550, 581, 602, 608, 656, 779
Alw21I GWGCWC 3 cut(s) 355, 552, 793
AlwI GGATC 1 cut(s) 248
AoxI GGCC 5 cut(s) 70, 378, 613, 671, 885
ApeKI GCWGC 2 cut(s) 293, 581
ApoI RAATTY 4 cut(s) 24, 136, 733, 804
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 4 cut(s) 408, 671, 713, 764
AsuHPI GGTGA 1 cut(s) 281
AvaII GGWCC 3 cut(s) 408, 713, 764
BalI TGGCCA 2 cut(s) 72, 615
BanII GRGCYC 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 3 cut(s) 355, 552, 793
BbvI GCAGC 2 cut(s) 305, 568
BccI CCATC 2 cut(s) 196, 653
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 1 cut(s) 412
BfaI CTAG 3 cut(s) 578, 653, 872
BfmI CTRYAG 1 cut(s) 832
BfuAI ACCTGC 2 cut(s) 293, 724
BglII AGATCT 1 cut(s) 496
BisI GCNGC 2 cut(s) 294, 582
BlsI GCNGC 2 cut(s) 295, 583
Bme1390I CCNGG 1 cut(s) 412
Bme18I GGWCC 3 cut(s) 408, 713, 764
BmgT120I GGNCC 4 cut(s) 408, 671, 713, 764
BmiI GGNNCC 2 cut(s) 417, 714
BmrFI CCNGG 1 cut(s) 412
BmrI ACTGGG 3 cut(s) 452, 719, 834
BmsI GCATC 1 cut(s) 809
BmuI ACTGGG 3 cut(s) 452, 719, 834
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 2 cut(s) 146, 305
BpuEI CTTGAG 2 cut(s) 151, 588
BsaJI CCNNGG 3 cut(s) 404, 411, 882
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 680, 773
Bse118I RCCGGY 1 cut(s) 785
Bse1I ACTGG 4 cut(s) 66, 447, 714, 840
Bse3DI GCAATG 3 cut(s) 114, 552, 639
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 404, 411, 882
BseLI CCNNNNNNNGG 3 cut(s) 94, 680, 773
BseMI GCAATG 3 cut(s) 114, 552, 639
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 4 cut(s) 66, 447, 714, 840
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 2 cut(s) 305, 568
BseYI CCCAGC 1 cut(s) 697
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 186, 188
BshFI GGCC 5 cut(s) 72, 380, 615, 673, 887
BsiHKAI GWGCWC 3 cut(s) 355, 552, 793
BsiSI CCGG 2 cut(s) 348, 786
BslFI GGGAC 3 cut(s) 223, 726, 822
BslI CCNNNNNNNGG 3 cut(s) 94, 680, 773
BsmFI GGGAC 3 cut(s) 223, 726, 822
BsnI GGCC 5 cut(s) 72, 380, 615, 673, 887
Bsp1286I GDGCHC 3 cut(s) 355, 552, 793
Bsp143I GATC 2 cut(s) 240, 496
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 2 cut(s) 188, 824
BspANI GGCC 5 cut(s) 72, 380, 615, 673, 887
BspCNI CTCAG 1 cut(s) 190
BspFNI CGCG 2 cut(s) 186, 188
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 2 cut(s) 417, 714
BspMAI CTGCAG 1 cut(s) 836
BspMI ACCTGC 2 cut(s) 293, 724
BspPI GGATC 1 cut(s) 248
BsrDI GCAATG 3 cut(s) 114, 552, 639
BsrFI RCCGGY 1 cut(s) 785
BsrI ACTGG 4 cut(s) 66, 447, 714, 840
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 3 cut(s) 404, 411, 882
BssMI GATC 2 cut(s) 240, 496
BssT1I CCWWGG 2 cut(s) 404, 882
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 313, 772
Bst6I CTCTTC 2 cut(s) 43, 853
BstC8I GCNNGC 2 cut(s) 250, 824
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 404
BstENI CCTNNNNNAGG 1 cut(s) 678
BstFNI CGCG 2 cut(s) 186, 188
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 2 cut(s) 243, 499
BstMBI GATC 2 cut(s) 240, 496
BstMWI GCNNNNNNNGC 3 cut(s) 194, 299, 893
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 410
BstSFI CTRYAG 1 cut(s) 832
BstUI CGCG 2 cut(s) 186, 188
BstV1I GCAGC 2 cut(s) 305, 568
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 1 cut(s) 496
BsuRI GGCC 5 cut(s) 72, 380, 615, 673, 887
BtgI CCRYGG 1 cut(s) 404
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 4 cut(s) 73, 440, 515, 707
BveI ACCTGC 2 cut(s) 293, 724
Cac8I GCNNGC 2 cut(s) 250, 824
CciI TCATGA 1 cut(s) 564
CfoI GCGC 1 cut(s) 188
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 4 cut(s) 408, 671, 713, 764
CseI GACGC 1 cut(s) 573
Csp6I GTAC 2 cut(s) 182, 659
CviAII CATG 5 cut(s) 356, 405, 462, 565, 729
CviQI GTAC 2 cut(s) 182, 659
DdeI CTNAG 1 cut(s) 198
DpnI GATC 2 cut(s) 242, 498
DpnII GATC 2 cut(s) 240, 496
EaeI YGGCCR 2 cut(s) 70, 613
Eam1104I CTCTTC 2 cut(s) 43, 853
EarI CTCTTC 2 cut(s) 43, 853
Ecl136II GAGCTC 1 cut(s) 550
Eco130I CCWWGG 2 cut(s) 404, 882
Eco24I GRGCYC 1 cut(s) 552
Eco47I GGWCC 3 cut(s) 408, 713, 764
Eco53kI GAGCTC 1 cut(s) 550
EcoICRI GAGCTC 1 cut(s) 550
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 2 cut(s) 671, 713
EcoRII CCWGG 1 cut(s) 410
EcoT14I CCWWGG 2 cut(s) 404, 882
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 2 cut(s) 404, 882
FaeI CATG 5 cut(s) 359, 408, 465, 568, 732
FalI AAGNNNNNCTT 4 cut(s) 264, 296, 514, 546
FaqI GGGAC 3 cut(s) 223, 726, 822
FatI CATG 5 cut(s) 355, 404, 461, 564, 728
FauI CCCGC 1 cut(s) 831
FauNDI CATATG 1 cut(s) 746
Fnu4HI GCNGC 2 cut(s) 294, 582
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 2 cut(s) 294, 582
FspBI CTAG 3 cut(s) 578, 653, 872
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 294, 582
GsaI CCCAGC 1 cut(s) 701
GsuI CTGGAG 2 cut(s) 146, 305
HaeIII GGCC 5 cut(s) 72, 380, 615, 673, 887
HapII CCGG 2 cut(s) 348, 786
HgaI GACGC 1 cut(s) 573
HhaI GCGC 1 cut(s) 188
Hin1II CATG 5 cut(s) 359, 408, 465, 568, 732
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HpaII CCGG 2 cut(s) 348, 786
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 2 cut(s) 361, 764
Hpy188I TCNGA 1 cut(s) 650
Hpy188III TCNNGA 7 cut(s) 130, 163, 220, 284, 565, 624, 681
Hpy8I GTNNAC 2 cut(s) 361, 764
HpyAV CCTTC 3 cut(s) 400, 465, 547
HpyCH4III ACNGT 2 cut(s) 313, 772
HpyCH4V TGCA 7 cut(s) 57, 107, 293, 520, 557, 719, 834
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 299, 893
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 5 cut(s) 359, 408, 465, 568, 732
HspAI GCGC 1 cut(s) 186
Kzo9I GATC 2 cut(s) 240, 496
LmnI GCTCC 2 cut(s) 776, 893
Lsp1109I GCAGC 2 cut(s) 305, 568
LweI GCATC 1 cut(s) 809
MaeI CTAG 3 cut(s) 578, 653, 872
MaeIII GTNAC 3 cut(s) 370, 705, 842
MalI GATC 2 cut(s) 242, 498
MboI GATC 2 cut(s) 240, 496
MboII GAAGA 5 cut(s) 30, 272, 800, 806, 840
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 3 cut(s) 355, 552, 793
MlsI TGGCCA 2 cut(s) 72, 615
MluCI AATT 5 cut(s) 24, 136, 720, 733, 804
MluNI TGGCCA 2 cut(s) 72, 615
MmeI TCCRAC 1 cut(s) 754
MnlI CCTC 5 cut(s) 46, 429, 512, 677, 862
Mox20I TGGCCA 2 cut(s) 72, 615
MscI TGGCCA 2 cut(s) 72, 615
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 1 cut(s) 569
Msp20I TGGCCA 2 cut(s) 72, 615
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 2 cut(s) 348, 786
MspR9I CCNGG 1 cut(s) 412
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 1 cut(s) 412
MvnI CGCG 2 cut(s) 186, 188
MwoI GCNNNNNNNGC 3 cut(s) 194, 299, 893
NcoI CCATGG 1 cut(s) 404
NdeI CATATG 1 cut(s) 746
NdeII GATC 2 cut(s) 240, 496
NlaIII CATG 5 cut(s) 359, 408, 465, 568, 732
NlaIV GGNNCC 2 cut(s) 417, 714
NmuCI GTSAC 2 cut(s) 370, 705
NspI RCATGY 1 cut(s) 465
PagI TCATGA 1 cut(s) 564
PciI ACATGT 1 cut(s) 461
PflMI CCANNNNNTGG 2 cut(s) 94, 773
PkrI GCNGC 2 cut(s) 295, 583
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 1 cut(s) 461
Psp124BI GAGCTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 410
PspFI CCCAGC 1 cut(s) 697
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 2 cut(s) 417, 714
PspPI GGNCC 4 cut(s) 408, 671, 713, 764
PspPPI RGGWCCY 1 cut(s) 713
PstI CTGCAG 1 cut(s) 836
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 2 cut(s) 183, 660
RsaNI GTAC 2 cut(s) 182, 659
RseI CAYNNNNRTG 1 cut(s) 569
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 2 cut(s) 294, 582
Sau3AI GATC 2 cut(s) 240, 496
Sau96I GGNCC 4 cut(s) 408, 671, 713, 764
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 3 cut(s) 355, 552, 793
SfaNI GCATC 1 cut(s) 809
SfcI CTRYAG 1 cut(s) 832
SgrAI CRCCGGYG 1 cut(s) 785
SinI GGWCC 3 cut(s) 408, 713, 764
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 2 cut(s) 130, 603
SmoI CTYRAG 2 cut(s) 130, 603
Sse9I AATT 5 cut(s) 24, 136, 720, 733, 804
SsiI CCGC 2 cut(s) 188, 824
SspMI CTAG 3 cut(s) 578, 653, 872
SstI GAGCTC 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 2 cut(s) 404, 882
TaaI ACNGT 2 cut(s) 313, 772
TaqI TCGA 1 cut(s) 817
TaqII GACCGA 1 cut(s) 383
TasI AATT 5 cut(s) 24, 136, 720, 733, 804
TatI WGTACW 1 cut(s) 658
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 4 cut(s) 73, 447, 522, 714
TseFI GTSAC 2 cut(s) 370, 705
TseI GCWGC 2 cut(s) 293, 581
Tsp45I GTSAC 2 cut(s) 370, 705
TspDTI ATGAA 7 cut(s) 17, 36, 278, 553, 707, 717, 800
TspRI CASTG 4 cut(s) 73, 447, 522, 714
Van91I CCANNNNNTGG 2 cut(s) 94, 773
VpaK11BI GGWCC 3 cut(s) 408, 713, 764
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 4 cut(s) 24, 136, 733, 804
XceI RCATGY 1 cut(s) 465
XspI CTAG 3 cut(s) 578, 653, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.