Rh4BG218700

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014604
Physical Location & Seq
Forward (+)
37898233 .. 37900819
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG218700.1

Sequence Viewer

Length: 666 bp
ATGAAAGGATTATATGAAGAGGCCAAACCATCACCCCAAATTTCAAACTCCGCGTCAACTAGCGGCCATTCTAAAGAAGATGATCCAGAAATGGGTTCCAATGCATTGTCAAACAGTGCAGATCATCATGTCTTGAACAACTCTGCCTTCTTCTGTTTTCCAAGCGGAATGAATTTAAATCTCGTGCGTGCGGTTGTGGCGGAGATGGTTGGAACTTTTATCTTGATGTTCTGTGTATGTGGGATCATAGCAAGCACAGAGCTGCTGAGAGGAGATGTAGGTCTTATGGAGTATGCATCCACAGCGGGTTTAACAGTTGTAGTTGTGATTTTCTCCGTAGGCTCCATCTCTGGTGCTCATGTTAACCCTGCTGTCACAGTAGCCTTTGCAACCTTTGGTCATTTCCCATGGTCCAGGGTTCCTCTATACACATTGGCACAAACATTGGGTTCTGTTCTGGCAGCATTCGTTGGAAAGCTTGTGTATGGCATAAAACCAGATCTCATGATCACCAAACCAGTGCAAGGATGTGTTTCTGCCTTCTGGGTTGAGCTTATTGCGACTTTCATTGTTTTGTTCCTTGCTGCTTCTATGACACATCATGCTCAAGCTGTACGGAAAAACTCTCCATCTGTATTTTGTCCTTTAATTTGCGCTTGCATTTGA

Protein Analysis

221

Amino Acids

23.42

Weight (kDa)

6.35

Isoelectric Point (pI)

22.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 59 - 209 1e-26 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014604)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06100 AT3G06100 AT3G06100
fragaria_vesca FvH4_4g14730 FvH4_4g14730
malus_domestica MD16G1185600.v1.1
prunus_persica Prupe.1G152300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0417871
rosa_laevigata RLG00000007891
rosa_multiflora Rmu_sc0004911.1_g000004
rosa_roxburghii Rroxscaffold_5G00361290
rosa_rugosa Rorug04G0150100
rosa_samantha Rh4AG212300 Rh4BG218700 Rh4CG223300 Rh4DG210200
rosa_wichuraiana Rw4G018020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 53
AciI CCGC 6 cut(s) 51, 63, 165, 191, 200, 305
AclWI GGATC 2 cut(s) 77, 251
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 39, 172
AfaI GTAC 1 cut(s) 615
AfiI CCNNNNNNNGG 2 cut(s) 92, 524
AgsI TTSAA 2 cut(s) 45, 136
AjnI CCWGG 1 cut(s) 413
AluBI AGCT 4 cut(s) 262, 478, 553, 611
AluI AGCT 4 cut(s) 262, 478, 553, 611
Alw21I GWGCWC 1 cut(s) 358
AlwI GGATC 2 cut(s) 77, 251
AoxI GGCC 2 cut(s) 21, 64
ApeKI GCWGC 3 cut(s) 262, 461, 584
ApoI RAATTY 2 cut(s) 39, 172
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AspLEI GCGC 1 cut(s) 656
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 24, 502
AvaII GGWCC 1 cut(s) 411
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 249, 473, 571
BccI CCATC 4 cut(s) 37, 199, 353, 637
BciT130I CCWGG 1 cut(s) 415
BclI TGATCA 1 cut(s) 507
BfaI CTAG 1 cut(s) 60
BglII AGATCT 1 cut(s) 499
BisI GCNGC 4 cut(s) 64, 263, 462, 585
BlsI GCNGC 4 cut(s) 65, 264, 463, 586
Bme1390I CCNGG 1 cut(s) 415
Bme18I GGWCC 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 3 cut(s) 97, 343, 420
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 591
BsaJI CCNNGG 2 cut(s) 407, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 524
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 415
BseDI CCNNGG 2 cut(s) 407, 414
BseGI GGATG 2 cut(s) 296, 533
BseLI CCNNNNNNNGG 2 cut(s) 92, 524
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 3 cut(s) 249, 473, 571
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 23, 66
BsiHKAI GWGCWC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 92, 524
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 2 cut(s) 23, 66
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 5 cut(s) 82, 121, 243, 499, 507
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 6 cut(s) 51, 63, 165, 191, 200, 305
BspANI GGCC 2 cut(s) 23, 66
BspCNI CTCAG 1 cut(s) 258
BspFNI CGCG 1 cut(s) 53
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 3 cut(s) 97, 343, 420
BspPI GGATC 2 cut(s) 77, 251
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 407, 414
BssMI GATC 5 cut(s) 82, 121, 243, 499, 507
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 407
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 3 cut(s) 116, 316, 379
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 3 cut(s) 189, 253, 658
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 2 cut(s) 296, 533
BstFNI CGCG 1 cut(s) 53
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 5 cut(s) 85, 124, 246, 502, 510
BstMBI GATC 5 cut(s) 82, 121, 243, 499, 507
BstMWI GCNNNNNNNGC 2 cut(s) 197, 302
BstNI CCWGG 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 413
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 3 cut(s) 249, 473, 571
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 2 cut(s) 23, 66
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 2 cut(s) 296, 533
BtsIMutI CAGTG 2 cut(s) 121, 525
Cac8I GCNNGC 3 cut(s) 189, 253, 658
CciI TCATGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 656
Cfr13I GGNCC 1 cut(s) 411
CseI GACGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 614
CviAII CATG 5 cut(s) 128, 359, 408, 505, 602
CviJI RGCY 8 cut(s) 23, 66, 262, 342, 383, 478, 553, 611
CviKI_1 RGCY 8 cut(s) 23, 66, 262, 342, 383, 478, 553, 611
CviQI GTAC 1 cut(s) 614
DdeI CTNAG 1 cut(s) 266
DpnI GATC 5 cut(s) 84, 123, 245, 501, 509
DpnII GATC 5 cut(s) 82, 121, 243, 499, 507
DraI TTTAAA 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 407
EcoT22I ATGCAT 2 cut(s) 106, 298
ErhI CCWWGG 1 cut(s) 407
FaeI CATG 5 cut(s) 131, 362, 411, 508, 605
FatI CATG 5 cut(s) 127, 358, 407, 504, 601
FauI CCCGC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 4 cut(s) 64, 263, 462, 585
FokI GGATG 2 cut(s) 283, 540
Fsp4HI GCNGC 4 cut(s) 64, 263, 462, 585
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 655
GluI GCNGC 4 cut(s) 64, 263, 462, 585
HaeIII GGCC 2 cut(s) 23, 66
HgaI GACGC 1 cut(s) 42
HhaI GCGC 1 cut(s) 656
Hin1II CATG 5 cut(s) 131, 362, 411, 508, 605
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HincII GTYRAC 2 cut(s) 57, 364
HindII GTYRAC 2 cut(s) 57, 364
HindIII AAGCTT 1 cut(s) 476
HpaI GTTAAC 1 cut(s) 364
HphI GGTGA 2 cut(s) 24, 502
Hpy166II GTNNAC 2 cut(s) 57, 364
Hpy188III TCNNGA 4 cut(s) 86, 133, 223, 505
Hpy8I GTNNAC 2 cut(s) 57, 364
HpyAV CCTTC 2 cut(s) 157, 550
HpyCH4III ACNGT 3 cut(s) 116, 316, 379
HpyCH4V TGCA 6 cut(s) 104, 119, 296, 389, 523, 660
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 302
HpyF3I CTNAG 1 cut(s) 266
Hsp92II CATG 5 cut(s) 131, 362, 411, 508, 605
HspAI GCGC 1 cut(s) 654
Ksp22I TGATCA 1 cut(s) 507
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 5 cut(s) 82, 121, 243, 499, 507
LmnI GCTCC 1 cut(s) 347
LpnPI CCDG 9 cut(s) 99, 336, 381, 400, 427, 443, 510, 529, 531
Lsp1109I GCAGC 3 cut(s) 249, 473, 571
LweI GCATC 1 cut(s) 305
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 373
MalI GATC 5 cut(s) 84, 123, 245, 501, 509
MboI GATC 5 cut(s) 82, 121, 243, 499, 507
MboII GAAGA 3 cut(s) 29, 89, 142
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 3 cut(s) 39, 172, 648
MmeI TCCRAC 2 cut(s) 190, 451
MnlI CCTC 3 cut(s) 13, 263, 432
Mph1103I ATGCAT 2 cut(s) 106, 298
MseI TTAA 4 cut(s) 176, 311, 363, 647
MspA1I CMGCKG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 415
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 415
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 197, 302
NcoI CCATGG 1 cut(s) 407
NdeII GATC 5 cut(s) 82, 121, 243, 499, 507
NlaIII CATG 5 cut(s) 131, 362, 411, 508, 605
NlaIV GGNNCC 3 cut(s) 97, 343, 420
NmuCI GTSAC 1 cut(s) 373
NsiI ATGCAT 2 cut(s) 106, 298
PagI TCATGA 1 cut(s) 504
PctI GAATGC 1 cut(s) 464
PkrI GCNGC 4 cut(s) 65, 264, 463, 586
Psp6I CCWGG 1 cut(s) 413
PspGI CCWGG 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 97, 343, 420
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 1 cut(s) 615
RsaNI GTAC 1 cut(s) 614
SaqAI TTAA 4 cut(s) 176, 311, 363, 647
SatI GCNGC 4 cut(s) 64, 263, 462, 585
Sau3AI GATC 5 cut(s) 82, 121, 243, 499, 507
Sau96I GGNCC 1 cut(s) 411
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 1 cut(s) 358
SetI ASST 6 cut(s) 264, 283, 395, 480, 555, 613
SfaNI GCATC 1 cut(s) 305
SinI GGWCC 1 cut(s) 411
SmiI ATTTAAAT 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
Sse9I AATT 3 cut(s) 39, 172, 648
SsiI CCGC 6 cut(s) 51, 63, 165, 191, 200, 305
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 413
StyI CCWWGG 1 cut(s) 407
SwaI ATTTAAAT 1 cut(s) 177
TaaI ACNGT 3 cut(s) 116, 316, 379
TasI AATT 3 cut(s) 39, 172, 648
TauI GCSGC 1 cut(s) 66
Tru1I TTAA 4 cut(s) 176, 311, 363, 647
Tru9I TTAA 4 cut(s) 176, 311, 363, 647
TscAI CASTG 2 cut(s) 121, 525
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 3 cut(s) 262, 461, 584
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 4 cut(s) 17, 30, 185, 556
TspGWI ACGGA 2 cut(s) 325, 631
TspRI CASTG 2 cut(s) 121, 525
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 2 cut(s) 39, 172
XspI CTAG 1 cut(s) 60
Zsp2I ATGCAT 2 cut(s) 106, 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.