MD16G1188800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016049
Physical Location & Seq
Reverse (-)
16392366 .. 16393091
726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1188800.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGGATCCCTAGAAATTGTTCAGGCAACCCCTAGATCCGTCGACATGTCTTCGAGTCCGCGGATTTCGTTCTCCGCCGAGTTCCTCGACGAGAACAACTTCATCTCCATCACCCCGAATTTGCGCGGCGAAGTACAAGATAAAAAAATGGAGGGTGGTGATCACCAAAAGGTACGTAACCCGGATTTTGAGTTTCTCTCGAGCAATGTGAGTAGCCATGCCATGTTGTCTGCAGATGAGCTATTTTTCGAAGGGAAGCTCCTTCCCTTTTGGCAAAAGCAGCACGCCGAACGGCTCACGAAGCTCAACCTCAACACCAAGGACGTCGAGGGAGACGAGAATGAGGAGGGGGTGAACAAGGAGGAGAGCCGGGGGAGTTGGTTTGTGGACGACGATCCATCTCCAAGGCCGCCTAAGTGCACGGTTTTGTGGAAGGAGCTACTGAGACTGAAGAAGCAACGCGCTTCGACATTGTCCCCATCTTCCTCGTCCTCGTCTTCCTCTTCTTCCTCGAACTCGTTGGCGGATATAGCTACGACGACGGATCAAGAGAAGGAAGGGAACAAAGAGAAGTATATGAAGAGGATAAAGAAAGGGTTGGAACGAACAAGATCCGCAAGCATAAGAATAAGGCCGATGGTAAATGTGCCAATTTGCACGCATGTGAAGAGCAGTGCCTTGCCACCTTTGTTTCCTCTCAGGAAAGGAAGAGTACTAGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.11

Weight (kDa)

8.8

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016049)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05980
fragaria_vesca FvH4_4g15060
malus_domestica MD13G1188400.v1.1 MD16G1188800.v1.1
prunus_persica Prupe.1G155500_v2.0.a1
pyrus_communis pycom13g16270 pycom16g15880
rosa_chinensis RchiOBHm_Chr4g0416801
rosa_laevigata RLG00000007949
rosa_multiflora Rmu_co8456203.1_g000001 Rmu_sc0015663.1_g000002
rosa_roxburghii Rroxscaffold_5G00360680
rosa_rugosa Rorug04G0145000
rosa_samantha Rh4CG218500
rosa_wichuraiana Rw4G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 61, 126, 462
AciI CCGC 7 cut(s) 59, 61, 75, 126, 410, 524, 615
AclWI GGATC 5 cut(s) 13, 30, 389, 552, 606
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 3 cut(s) 135, 174, 714
AflIII ACRYGT 1 cut(s) 45
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 5 cut(s) 241, 259, 304, 439, 533
AluI AGCT 5 cut(s) 241, 259, 304, 439, 533
Alw21I GWGCWC 1 cut(s) 422
Alw26I GTCTC 2 cut(s) 327, 439
Alw44I GTGCAC 1 cut(s) 418
AlwI GGATC 5 cut(s) 13, 30, 389, 552, 606
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 2 cut(s) 407, 632
ApaLI GTGCAC 1 cut(s) 418
ApeKI GCWGC 1 cut(s) 280
ApoI RAATTY 1 cut(s) 118
Asp700I GAANNNNTTC 2 cut(s) 18, 98
AspLEI GCGC 2 cut(s) 126, 464
AsuC2I CCSGG 2 cut(s) 182, 371
AsuHPI GGTGA 4 cut(s) 103, 155, 170, 364
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 422
BamHI GGATCC 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 42, 489
Bbv12I GWGCWC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 292
BccI CCATC 4 cut(s) 116, 406, 487, 631
BceAI ACGGC 1 cut(s) 308
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 2 cut(s) 182, 371
BcoDI GTCTC 2 cut(s) 327, 439
BfaI CTAG 3 cut(s) 11, 33, 716
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 3 cut(s) 127, 281, 410
BlsI GCNGC 3 cut(s) 128, 282, 411
BmcAI AGTACT 1 cut(s) 714
Bme1390I CCNGG 2 cut(s) 182, 371
BmeT110I CYCGRG 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 2 cut(s) 182, 371
BpiI GAAGAC 2 cut(s) 42, 489
BplI GAGNNNNNCTC 2 cut(s) 182, 214
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 182, 371
BsaAI YACGTR 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 4 cut(s) 59, 318, 370, 404
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bse3DI GCAATG 1 cut(s) 211
BseDI CCNNGG 4 cut(s) 59, 318, 370, 404
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 434, 712
BseRI GAGGAG 2 cut(s) 359, 377
BseSI GKGCMC 1 cut(s) 422
BseXI GCAGC 1 cut(s) 292
Bsh1236I CGCG 3 cut(s) 61, 126, 462
BshFI GGCC 2 cut(s) 409, 634
BsiHKAI GWGCWC 1 cut(s) 422
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 2 cut(s) 182, 370
BslFI GGGAC 1 cut(s) 460
BsmAI GTCTC 2 cut(s) 327, 439
BsmBI CGTCTC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 460
BsnI GGCC 2 cut(s) 409, 634
BsoBI CYCGRG 1 cut(s) 199
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 422
Bsp143I GATC 6 cut(s) 5, 35, 160, 394, 544, 611
BspACI CCGC 7 cut(s) 59, 61, 75, 126, 410, 524, 615
BspANI GGCC 2 cut(s) 409, 634
BspCNI CTCAG 2 cut(s) 435, 711
BspFNI CGCG 3 cut(s) 61, 126, 462
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 5 cut(s) 13, 30, 389, 552, 606
BspQI GCTCTTC 1 cut(s) 662
BspT104I TTCGAA 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 211
BssECI CCNNGG 4 cut(s) 59, 318, 370, 404
BssMI GATC 6 cut(s) 5, 35, 160, 394, 544, 611
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 318, 404
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 4 cut(s) 508, 575, 662, 703
BstACI GRCGYC 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 285, 619, 659
BstDEI CTNAG 3 cut(s) 414, 443, 698
BstDSI CCRYGG 1 cut(s) 59
BstFNI CGCG 3 cut(s) 61, 126, 462
BstHHI GCGC 2 cut(s) 126, 464
BstKTI GATC 6 cut(s) 8, 38, 163, 397, 547, 614
BstMAI GTCTC 2 cut(s) 327, 439
BstMBI GATC 6 cut(s) 5, 35, 160, 394, 544, 611
BstMWI GCNNNNNNNGC 3 cut(s) 280, 301, 530
BstNSI RCATGY 2 cut(s) 49, 665
BstSCI CCNGG 2 cut(s) 180, 369
BstSFI CTRYAG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 422
BstSNI TACGTA 1 cut(s) 176
BstUI CGCG 3 cut(s) 61, 126, 462
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 2 cut(s) 42, 489
BstX2I RGATCY 3 cut(s) 5, 35, 611
BstYI RGATCY 3 cut(s) 5, 35, 611
BsuRI GGCC 2 cut(s) 409, 634
BtgI CCRYGG 1 cut(s) 59
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 3 cut(s) 285, 619, 659
CfoI GCGC 2 cut(s) 126, 464
Cfr42I CCGCGG 1 cut(s) 62
Csp6I GTAC 3 cut(s) 134, 173, 713
CviAII CATG 4 cut(s) 46, 218, 223, 662
CviQI GTAC 3 cut(s) 134, 173, 713
DdeI CTNAG 3 cut(s) 414, 443, 698
DpnI GATC 6 cut(s) 7, 37, 162, 396, 546, 613
DpnII GATC 6 cut(s) 5, 35, 160, 394, 544, 611
Eam1104I CTCTTC 4 cut(s) 508, 575, 662, 703
EarI CTCTTC 4 cut(s) 508, 575, 662, 703
EciI GGCGGA 2 cut(s) 64, 539
Eco105I TACGTA 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 318, 404
Eco57I CTGAAG 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 318, 404
ErhI CCWWGG 2 cut(s) 318, 404
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 4 cut(s) 49, 221, 226, 665
FaiI YATR 8 cut(s) 47, 219, 224, 530, 576, 578, 623, 663
FaqI GGGAC 1 cut(s) 460
FatI CATG 4 cut(s) 45, 217, 222, 661
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 127, 281, 410
Fsp4HI GCNGC 3 cut(s) 127, 281, 410
FspBI CTAG 3 cut(s) 11, 33, 716
GlaI GCGC 2 cut(s) 125, 463
GluI GCNGC 3 cut(s) 127, 281, 410
HaeIII GGCC 2 cut(s) 409, 634
HapII CCGG 2 cut(s) 182, 370
HhaI GCGC 2 cut(s) 126, 464
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 4 cut(s) 49, 221, 226, 665
Hin6I GCGC 2 cut(s) 124, 462
HinP1I GCGC 2 cut(s) 124, 462
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 1 cut(s) 55
HpaII CCGG 2 cut(s) 182, 370
HphI GGTGA 4 cut(s) 103, 155, 170, 364
Hpy166II GTNNAC 4 cut(s) 43, 355, 388, 420
Hpy188III TCNNGA 4 cut(s) 199, 298, 548, 700
Hpy8I GTNNAC 4 cut(s) 43, 355, 388, 420
Hpy99I CGWCG 6 cut(s) 44, 92, 329, 395, 541, 544
HpyAV CCTTC 5 cut(s) 245, 272, 427, 547, 551
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 2 cut(s) 175, 324
HpyCH4V TGCA 3 cut(s) 233, 420, 657
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 301, 530
HpyF3I CTNAG 3 cut(s) 414, 443, 698
HpySE526I ACGT 2 cut(s) 175, 324
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 4 cut(s) 49, 221, 226, 665
HspAI GCGC 2 cut(s) 124, 462
Ksp22I TGATCA 1 cut(s) 160
KspI CCGCGG 1 cut(s) 62
Kzo9I GATC 6 cut(s) 5, 35, 160, 394, 544, 611
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 2 cut(s) 264, 436
LpnPI CCDG 4 cut(s) 8, 195, 383, 685
Lsp1109I GCAGC 1 cut(s) 292
MaeI CTAG 3 cut(s) 11, 33, 716
MaeII ACGT 2 cut(s) 175, 324
MaeIII GTNAC 1 cut(s) 176
MalI GATC 6 cut(s) 7, 37, 162, 396, 546, 613
MboI GATC 6 cut(s) 5, 35, 160, 394, 544, 611
MboII GAAGA 9 cut(s) 42, 463, 474, 489, 495, 498, 592, 679, 720
MflI RGATCY 3 cut(s) 5, 35, 611
MhlI GDGCHC 1 cut(s) 422
MluCI AATT 3 cut(s) 15, 118, 651
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 579
MroXI GAANNNNTTC 2 cut(s) 18, 98
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 2 cut(s) 182, 370
MspR9I CCNGG 2 cut(s) 182, 371
MvnI CGCG 3 cut(s) 61, 126, 462
MwoI GCNNNNNNNGC 3 cut(s) 280, 301, 530
NciI CCSGG 2 cut(s) 182, 371
NdeII GATC 6 cut(s) 5, 35, 160, 394, 544, 611
NlaIII CATG 4 cut(s) 49, 221, 226, 665
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 1 cut(s) 103
NspI RCATGY 2 cut(s) 49, 665
NspV TTCGAA 1 cut(s) 249
OliI CACNNNNGTG 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 199
PciI ACATGT 1 cut(s) 45
PciSI GCTCTTC 1 cut(s) 662
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PdmI GAANNNNTTC 2 cut(s) 18, 98
PflFI GACNNNGTC 1 cut(s) 472
PkrI GCNGC 3 cut(s) 128, 282, 411
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
Ppu21I YACGTR 1 cut(s) 176
PscI ACATGT 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 7
PstI CTGCAG 1 cut(s) 235
PsuI RGATCY 3 cut(s) 5, 35, 611
PsyI GACNNNGTC 1 cut(s) 472
RsaI GTAC 3 cut(s) 135, 174, 714
RsaNI GTAC 3 cut(s) 134, 173, 713
RseI CAYNNNNRTG 1 cut(s) 662
SacII CCGCGG 1 cut(s) 62
SalI GTCGAC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 662
SatI GCNGC 3 cut(s) 127, 281, 410
Sau3AI GATC 6 cut(s) 5, 35, 160, 394, 544, 611
ScaI AGTACT 1 cut(s) 714
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 182, 371
SduI GDGCHC 1 cut(s) 422
SfcI CTRYAG 1 cut(s) 231
Sfr274I CTCGAG 1 cut(s) 199
Sfr303I CCGCGG 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 249
SgrBI CCGCGG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
SnaBI TACGTA 1 cut(s) 176
Sse9I AATT 3 cut(s) 15, 118, 651
SsiI CCGC 7 cut(s) 59, 61, 75, 126, 410, 524, 615
SspMI CTAG 3 cut(s) 11, 33, 716
StyD4I CCNGG 2 cut(s) 180, 369
StyI CCWWGG 2 cut(s) 318, 404
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 2 cut(s) 178, 327
TaqI TCGA 8 cut(s) 42, 53, 87, 200, 249, 327, 467, 512
TasI AATT 3 cut(s) 15, 118, 651
TatI WGTACW 2 cut(s) 133, 712
TauI GCSGC 2 cut(s) 129, 412
TscAI CASTG 1 cut(s) 679
TseI GCWGC 1 cut(s) 280
TspDTI ATGAA 2 cut(s) 91, 593
TspGWI ACGGA 2 cut(s) 28, 557
TspRI CASTG 1 cut(s) 679
Tth111I GACNNNGTC 1 cut(s) 472
VneI GTGCAC 1 cut(s) 418
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 49, 665
XhoI CTCGAG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 42
XmnI GAANNNNTTC 2 cut(s) 18, 98
XspI CTAG 3 cut(s) 11, 33, 716
ZraI GACGTC 1 cut(s) 325
ZrmI AGTACT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.