pycom13g16270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016049
Physical Location & Seq
Reverse (-)
11981587 .. 11982395
809 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16270.1

Sequence Viewer

Length: 741 bp
ATGGGGTCCCTAGAAATTGTTCCGGCAACCCCTATATCTGCCGACTCGATTTTGAGTCCGCGGATTTCATTCTCGGTCGAGTTTCTCGACGATGAGAACTTCATCTCCATCACCCCGAATTCGCACGGCGAAGTACAAGATAAAAAAATGGAGTGTGGTGATCACCAAAAGGTACGTAACCCGGATTTTGAGTTTCTCTCGAGCAATGCGAGCAGCCATGCCATGTTGTCTGCAGATGAGCTATTTTTCGATGGGAAGCTCCTACCCTTTTGGCAAAAGCAGCACGCCGAACAGCTCGCGAAGCTCAACCTCAAGACCAAGGATGTCGAGGGAGATGAGAATGAAGAGGTGGTGAACAAGGAGGAGAGAGCTACTAAGACTGAAGAAGCAACGCGCTTCATCATTGTCCCCGTCTTCCTCATCCTCGTCTTCCTCTTCTTCCTCGAACTTGTCGGCGGATGTAGCTACGAGGACGGATCGAGAGAAGGAAGGGAACAAAGAGAAGTATATGAAGAGGATAAAGAAAGGGTTGGAAAGAACAAGATCAGCAAGCATAAGAATAAGGCCAATGGTAAATGTGCCAATTTGCACACAGGTGAAGAGCAGTGCCTTCCCACCTTTGTTTCCTCTCAGAAAAGGAAGAGTGCTAGAGAGGTAGAGATGTTGGTCCGATCTGCAGGATTAATTGTCCAATGCGAGAATTTTACCGTGCTCCGGTACTGTATGACATGCACCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

27.92

Weight (kDa)

5.32

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016049)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05980
fragaria_vesca FvH4_4g15060
malus_domestica MD13G1188400.v1.1 MD16G1188800.v1.1
prunus_persica Prupe.1G155500_v2.0.a1
pyrus_communis pycom13g16270 pycom16g15880
rosa_chinensis RchiOBHm_Chr4g0416801
rosa_laevigata RLG00000007949
rosa_multiflora Rmu_co8456203.1_g000001 Rmu_sc0015663.1_g000002
rosa_roxburghii Rroxscaffold_5G00360680
rosa_rugosa Rorug04G0145000
rosa_samantha Rh4CG218500
rosa_wichuraiana Rw4G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 61, 299, 394
AciI CCGC 3 cut(s) 59, 61, 456
AclWI GGATC 1 cut(s) 484
AcsI RAATTY 2 cut(s) 118, 700
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 3 cut(s) 135, 174, 719
AfiI CCNNNNNNNGG 1 cut(s) 714
AleI CACNNNNGTG 1 cut(s) 594
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
AluBI AGCT 6 cut(s) 241, 259, 295, 304, 371, 465
AluI AGCT 6 cut(s) 241, 259, 295, 304, 371, 465
Alw21I GWGCWC 1 cut(s) 714
AlwI GGATC 1 cut(s) 484
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 1 cut(s) 564
ApeKI GCWGC 2 cut(s) 213, 280
ApoI RAATTY 2 cut(s) 118, 700
AseI ATTAAT 1 cut(s) 683
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 1 cut(s) 396
AspS9I GGNCC 2 cut(s) 6, 667
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 5 cut(s) 103, 155, 170, 364, 608
AvaI CYCGRG 1 cut(s) 199
AvaII GGWCC 2 cut(s) 6, 667
BbsI GAAGAC 2 cut(s) 406, 421
Bbv12I GWGCWC 1 cut(s) 714
BbvI GCAGC 2 cut(s) 225, 292
BccI CCATC 2 cut(s) 116, 245
BceAI ACGGC 1 cut(s) 142
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 1 cut(s) 182
BfaI CTAG 2 cut(s) 11, 648
BfmI CTRYAG 2 cut(s) 231, 675
BisI GCNGC 2 cut(s) 214, 281
BlsI GCNGC 2 cut(s) 215, 282
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 2 cut(s) 6, 667
BmeT110I CYCGRG 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 6, 667
BmiI GGNNCC 2 cut(s) 7, 8
BmrFI CCNGG 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 406, 421
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 182
BsaAI YACGTR 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 59, 318
BsaWI WCCGGW 1 cut(s) 714
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 1 cut(s) 714
Bse3DI GCAATG 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 59, 318
BseGI GGATG 3 cut(s) 328, 420, 464
BseLI CCNNNNNNNGG 1 cut(s) 714
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 644
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 225, 292
Bsh1236I CGCG 3 cut(s) 61, 299, 394
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 566
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 3 cut(s) 23, 182, 715
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 1 cut(s) 566
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 714
Bsp143I GATC 4 cut(s) 160, 476, 543, 671
Bsp68I TCGCGA 1 cut(s) 299
BspACI CCGC 3 cut(s) 59, 61, 456
BspANI GGCC 1 cut(s) 566
BspCNI CTCAG 1 cut(s) 643
BspFNI CGCG 3 cut(s) 61, 299, 394
BspLI GGNNCC 2 cut(s) 7, 8
BspMAI CTGCAG 2 cut(s) 235, 679
BspPI GGATC 1 cut(s) 484
BspQI GCTCTTC 1 cut(s) 594
BsrDI GCAATG 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 59, 318
BssMI GATC 4 cut(s) 160, 476, 543, 671
BssT1I CCWWGG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 709, 722
Bst6I CTCTTC 5 cut(s) 339, 440, 507, 594, 635
BstBAI YACGTR 1 cut(s) 176
BstC8I GCNNGC 4 cut(s) 211, 285, 297, 551
BstDEI CTNAG 2 cut(s) 375, 630
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 328, 420, 464
BstFNI CGCG 3 cut(s) 61, 299, 394
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 4 cut(s) 163, 479, 546, 674
BstMBI GATC 4 cut(s) 160, 476, 543, 671
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 4 cut(s) 210, 280, 301, 462
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 2 cut(s) 231, 675
BstSNI TACGTA 1 cut(s) 176
BstUI CGCG 3 cut(s) 61, 299, 394
BstV1I GCAGC 2 cut(s) 225, 292
BstV2I GAAGAC 2 cut(s) 406, 421
BsuRI GGCC 1 cut(s) 566
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 3 cut(s) 328, 420, 464
BtsI GCAGTG 1 cut(s) 611
BtsIMutI CAGTG 1 cut(s) 611
BtuMI TCGCGA 1 cut(s) 299
Cac8I GCNNGC 4 cut(s) 211, 285, 297, 551
CfoI GCGC 1 cut(s) 396
Cfr13I GGNCC 2 cut(s) 6, 667
Cfr42I CCGCGG 1 cut(s) 62
Csp6I GTAC 3 cut(s) 134, 173, 718
CviAII CATG 3 cut(s) 218, 223, 729
CviJI RGCY 8 cut(s) 216, 241, 259, 295, 304, 371, 465, 566
CviKI_1 RGCY 8 cut(s) 216, 241, 259, 295, 304, 371, 465, 566
CviQI GTAC 3 cut(s) 134, 173, 718
DdeI CTNAG 2 cut(s) 375, 630
DpnI GATC 4 cut(s) 162, 478, 545, 673
DpnII GATC 4 cut(s) 160, 476, 543, 671
Eam1104I CTCTTC 5 cut(s) 339, 440, 507, 594, 635
EarI CTCTTC 5 cut(s) 339, 440, 507, 594, 635
EciI GGCGGA 1 cut(s) 471
Eco105I TACGTA 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 6, 667
Eco57I CTGAAG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 3 cut(s) 221, 226, 732
FaiI YATR 8 cut(s) 35, 219, 224, 508, 510, 555, 725, 730
FaqI GGGAC 1 cut(s) 392
FatI CATG 3 cut(s) 217, 222, 728
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 214, 281
FokI GGATG 3 cut(s) 335, 407, 471
Fsp4HI GCNGC 2 cut(s) 214, 281
FspBI CTAG 2 cut(s) 11, 648
GlaI GCGC 1 cut(s) 395
GluI GCNGC 2 cut(s) 214, 281
HaeIII GGCC 1 cut(s) 566
HapII CCGG 3 cut(s) 23, 182, 715
HhaI GCGC 1 cut(s) 396
Hin1II CATG 3 cut(s) 221, 226, 732
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HinfI GANTC 2 cut(s) 44, 55
HpaII CCGG 3 cut(s) 23, 182, 715
HphI GGTGA 5 cut(s) 103, 155, 170, 364, 608
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 2 cut(s) 633, 671
Hpy188III TCNNGA 5 cut(s) 86, 199, 298, 313, 480
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 479, 483, 620
HpyCH4III ACNGT 2 cut(s) 709, 722
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 233, 589, 677, 732
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 280, 301, 462
HpyF3I CTNAG 2 cut(s) 375, 630
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 3 cut(s) 221, 226, 732
HspAI GCGC 1 cut(s) 394
KflI GGGWCCC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 160
KspI CCGCGG 1 cut(s) 62
Kzo9I GATC 4 cut(s) 160, 476, 543, 671
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 2 cut(s) 264, 717
LpnPI CCDG 5 cut(s) 36, 195, 579, 663, 728
Lsp1109I GCAGC 2 cut(s) 225, 292
MaeI CTAG 2 cut(s) 11, 648
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 176
MalI GATC 4 cut(s) 162, 478, 545, 673
MboI GATC 4 cut(s) 160, 476, 543, 671
MboII GAAGA 9 cut(s) 356, 395, 406, 421, 427, 430, 524, 611, 652
MhlI GDGCHC 1 cut(s) 714
MluCI AATT 5 cut(s) 15, 118, 583, 684, 700
MlyI GAGTC 2 cut(s) 38, 64
MmeI TCCRAC 1 cut(s) 511
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 1 cut(s) 594
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 3 cut(s) 23, 182, 715
MspR9I CCNGG 1 cut(s) 182
MvnI CGCG 3 cut(s) 61, 299, 394
MwoI GCNNNNNNNGC 4 cut(s) 210, 280, 301, 462
NciI CCSGG 1 cut(s) 182
NdeII GATC 4 cut(s) 160, 476, 543, 671
NlaIII CATG 3 cut(s) 221, 226, 732
NlaIV GGNNCC 2 cut(s) 7, 8
NruI TCGCGA 1 cut(s) 299
NspI RCATGY 1 cut(s) 732
OliI CACNNNNGTG 1 cut(s) 594
PaeR7I CTCGAG 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 594
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 206
PdmI GAANNNNTTC 1 cut(s) 18
PkrI GCNGC 2 cut(s) 215, 282
PleI GAGTC 2 cut(s) 38, 63
PpsI GAGTC 2 cut(s) 38, 63
Ppu21I YACGTR 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 6
PshBI ATTAAT 1 cut(s) 683
Psp5II RGGWCCY 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 7, 8
PspPI GGNCC 2 cut(s) 6, 667
PspPPI RGGWCCY 1 cut(s) 6
PstI CTGCAG 2 cut(s) 235, 679
RruI TCGCGA 1 cut(s) 299
RsaI GTAC 3 cut(s) 135, 174, 719
RsaNI GTAC 3 cut(s) 134, 173, 718
RseI CAYNNNNRTG 1 cut(s) 594
SacII CCGCGG 1 cut(s) 62
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 2 cut(s) 214, 281
Sau3AI GATC 4 cut(s) 160, 476, 543, 671
Sau96I GGNCC 2 cut(s) 6, 667
SchI GAGTC 2 cut(s) 38, 64
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 714
SfcI CTRYAG 2 cut(s) 231, 675
Sfr274I CTCGAG 1 cut(s) 199
Sfr303I CCGCGG 1 cut(s) 62
SgrBI CCGCGG 1 cut(s) 62
SinI GGWCC 2 cut(s) 6, 667
SlaI CTCGAG 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 199, 311
SmoI CTYRAG 2 cut(s) 199, 311
SnaBI TACGTA 1 cut(s) 176
Sse9I AATT 5 cut(s) 15, 118, 583, 684, 700
SsiI CCGC 3 cut(s) 59, 61, 456
SspMI CTAG 2 cut(s) 11, 648
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 709, 722
TaiI ACGT 1 cut(s) 178
TaqI TCGA 8 cut(s) 47, 78, 87, 200, 249, 327, 444, 479
TaqII GACCGA 1 cut(s) 64
TasI AATT 5 cut(s) 15, 118, 583, 684, 700
TatI WGTACW 1 cut(s) 133
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 1 cut(s) 611
TseI GCWGC 2 cut(s) 213, 280
TspDTI ATGAA 5 cut(s) 57, 91, 357, 388, 525
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 6, 667
VspI ATTAAT 1 cut(s) 683
XapI RAATTY 2 cut(s) 118, 700
XceI RCATGY 1 cut(s) 732
XhoI CTCGAG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 2 cut(s) 11, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.