Rw4G017550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016049
Physical Location & Seq
Forward (+)
39520979 .. 39521697
719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017550.1

Sequence Viewer

Length: 684 bp
ATGGTATCCCTGGAAGTTGTTCAGACAACCTCTAGATCCATCGAGCCATCCTCGAGTCCCCGGATTTCTTTCTCTACCGATTTCCTCGACGAAAATGACTTCATCACTATTAGCCCAAATCCTCACGGTGAACTTCAAGACAGGAAAATGGAGTGTGATCAGAAGGCAAGAAATGCAGACTTTGAGTTTCTCTCAAACAATGTGAGTAGTCATGCCATGTTAACTGCAGATGAGCTCTTCTTTGAAGGAAAGCTCCTTCCCTTTTGGCAAAAGCAGCATGCCGAAAGGCTCAACAAGATTCGTCTTAAAACAAAGGACGATGAGATAAGCGAGGAAGAGGAAGAGGTAGTGAACAAGGAGGAAAGTAGAGGAAACTGGTTTGTCGATGATGACCCATCTCCAAGGCCACCGAAGTGTACTGTTTTATGGAAAGAGTTGCTCAGGTTGAAGAAGCAACGTGCTTCAACTCTCTCACCCTCCTCTTCATCTCCCACAGCAGATCAAGAGAAGGAGCGGATGGGAAACAAAGAGAAGTACATGAAGAGGATAAAGAAAGGCTTAGAGAGAACACGATCAGCCAGCATAAGAATAAGGCCAATGATTAATGTGCCGATTTGCACACAGATGAAGAACAGTGCGTTACCACCTTTGTTTCCTCTCAGAAAAGGAAGACTAGATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

26.25

Weight (kDa)

8.7

Isoelectric Point (pI)

64.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016049)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05980
fragaria_vesca FvH4_4g15060
malus_domestica MD13G1188400.v1.1 MD16G1188800.v1.1
prunus_persica Prupe.1G155500_v2.0.a1
pyrus_communis pycom13g16270 pycom16g15880
rosa_chinensis RchiOBHm_Chr4g0416801
rosa_laevigata RLG00000007949
rosa_multiflora Rmu_co8456203.1_g000001 Rmu_sc0015663.1_g000002
rosa_roxburghii Rroxscaffold_5G00360680
rosa_rugosa Rorug04G0145000
rosa_samantha Rh4CG218500
rosa_wichuraiana Rw4G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 514
AciI CCGC 1 cut(s) 514
AclWI GGATC 1 cut(s) 30
AfaI GTAC 2 cut(s) 418, 536
AgsI TTSAA 4 cut(s) 137, 245, 448, 465
AjnI CCWGG 1 cut(s) 9
AleI CACNNNNGTG 1 cut(s) 412
AluBI AGCT 2 cut(s) 235, 253
AluI AGCT 2 cut(s) 235, 253
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 1 cut(s) 30
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 2 cut(s) 404, 593
ApeKI GCWGC 1 cut(s) 274
AseI ATTAAT 1 cut(s) 603
Asp700I GAANNNNTTC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 140, 465
AvaI CYCGRG 1 cut(s) 52
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 676
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 286
BccI CCATC 4 cut(s) 47, 55, 403, 511
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 16
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 1 cut(s) 61
BfaI CTAG 2 cut(s) 33, 674
BfmI CTRYAG 1 cut(s) 225
BfuI GTATCC 1 cut(s) 16
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
Bme1390I CCNGG 2 cut(s) 11, 61
BmeT110I CYCGRG 1 cut(s) 52
BmrFI CCNGG 2 cut(s) 11, 61
BpiI GAAGAC 1 cut(s) 676
BplI GAGNNNNNCTC 4 cut(s) 35, 67, 176, 208
Bpu10I CCTNAGC 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 9, 59, 401
Bse1I ACTGG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 9, 59, 401
BseGI GGATG 2 cut(s) 47, 522
BseMII CTCAG 2 cut(s) 454, 673
BseNI ACTGG 1 cut(s) 380
BseRI GAGGAG 1 cut(s) 469
BseXI GCAGC 1 cut(s) 286
BshFI GGCC 2 cut(s) 406, 595
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 1 cut(s) 61
BslFI GGGAC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 42
BsnI GGCC 2 cut(s) 406, 595
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 4 cut(s) 35, 157, 499, 572
BspACI CCGC 1 cut(s) 514
BspANI GGCC 2 cut(s) 406, 595
BspCNI CTCAG 2 cut(s) 453, 672
BspMAI CTGCAG 1 cut(s) 229
BspPI GGATC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 514
BsrI ACTGG 1 cut(s) 380
BssECI CCNNGG 3 cut(s) 9, 59, 401
BssMI GATC 4 cut(s) 35, 157, 499, 572
BssT1I CCWWGG 1 cut(s) 401
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 3 cut(s) 128, 421, 635
Bst6I CTCTTC 5 cut(s) 242, 330, 336, 487, 536
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 279, 580
BstDEI CTNAG 3 cut(s) 440, 559, 659
BstF5I GGATG 2 cut(s) 47, 522
BstKTI GATC 4 cut(s) 38, 160, 502, 575
BstMBI GATC 4 cut(s) 35, 157, 499, 572
BstMWI GCNNNNNNNGC 2 cut(s) 173, 274
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 9, 59
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 676
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuI GTATCC 1 cut(s) 16
BsuRI GGCC 2 cut(s) 406, 595
BtsCI GGATG 2 cut(s) 47, 522
BtsIMutI CAGTG 1 cut(s) 640
Cac8I GCNNGC 2 cut(s) 279, 580
Csp6I GTAC 2 cut(s) 417, 535
CviAII CATG 4 cut(s) 212, 217, 278, 538
CviJI RGCY 9 cut(s) 46, 114, 235, 253, 289, 406, 558, 578, 595
CviKI_1 RGCY 9 cut(s) 46, 114, 235, 253, 289, 406, 558, 578, 595
CviQI GTAC 2 cut(s) 417, 535
DdeI CTNAG 3 cut(s) 440, 559, 659
DpnI GATC 4 cut(s) 37, 159, 501, 574
DpnII GATC 4 cut(s) 35, 157, 499, 572
Eam1104I CTCTTC 5 cut(s) 242, 330, 336, 487, 536
EarI CTCTTC 5 cut(s) 242, 330, 336, 487, 536
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 401
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 401
FaeI CATG 4 cut(s) 215, 220, 281, 541
FaiI YATR 6 cut(s) 213, 218, 279, 427, 539, 584
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 1 cut(s) 42
FatI CATG 4 cut(s) 211, 216, 277, 537
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 275
FokI GGATG 2 cut(s) 34, 529
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 275
FspBI CTAG 2 cut(s) 33, 674
GluI GCNGC 1 cut(s) 275
HaeIII GGCC 2 cut(s) 406, 595
HapII CCGG 1 cut(s) 61
Hin1II CATG 4 cut(s) 215, 220, 281, 541
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 2 cut(s) 55, 298
HpaI GTTAAC 1 cut(s) 222
HpaII CCGG 1 cut(s) 61
HphI GGTGA 2 cut(s) 140, 465
Hpy166II GTNNAC 4 cut(s) 131, 222, 352, 417
Hpy188I TCNGA 3 cut(s) 24, 162, 662
Hpy188III TCNNGA 3 cut(s) 33, 137, 503
Hpy8I GTNNAC 4 cut(s) 131, 222, 352, 417
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 157, 239, 266, 502
HpyCH4III ACNGT 3 cut(s) 128, 421, 635
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 3 cut(s) 176, 227, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 274
HpyF3I CTNAG 3 cut(s) 440, 559, 659
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 4 cut(s) 215, 220, 281, 541
Ksp22I TGATCA 1 cut(s) 157
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 4 cut(s) 35, 157, 499, 572
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 2 cut(s) 258, 511
LpnPI CCDG 6 cut(s) 23, 74, 127, 361, 427, 592
Lsp1109I GCAGC 1 cut(s) 286
MaeI CTAG 2 cut(s) 33, 674
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 1 cut(s) 639
MalI GATC 4 cut(s) 37, 159, 501, 574
MbiI CCGCTC 1 cut(s) 514
MboI GATC 4 cut(s) 35, 157, 499, 572
MboII GAAGA 8 cut(s) 229, 347, 353, 460, 474, 553, 640, 681
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 237
MlyI GAGTC 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 3 cut(s) 221, 306, 603
MslI CAYNNNNRTG 2 cut(s) 412, 623
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 2 cut(s) 11, 61
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 2 cut(s) 173, 274
NciI CCSGG 1 cut(s) 61
NdeII GATC 4 cut(s) 35, 157, 499, 572
NlaIII CATG 4 cut(s) 215, 220, 281, 541
NspI RCATGY 1 cut(s) 281
OliI CACNNNNGTG 1 cut(s) 412
PaeI GCATGC 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 1 cut(s) 276
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PshBI ATTAAT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspXI VCTCGAGB 1 cut(s) 52
PstI CTGCAG 1 cut(s) 229
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 2 cut(s) 418, 536
RsaNI GTAC 2 cut(s) 417, 535
RseI CAYNNNNRTG 2 cut(s) 412, 623
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 3 cut(s) 221, 306, 603
SatI GCNGC 1 cut(s) 275
Sau3AI GATC 4 cut(s) 35, 157, 499, 572
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 11, 61
SduI GDGCHC 1 cut(s) 237
SetI ASST 8 cut(s) 32, 237, 255, 348, 446, 460, 649, 683
SfcI CTRYAG 1 cut(s) 225
Sfr274I CTCGAG 1 cut(s) 52
SlaI CTCGAG 1 cut(s) 52
SmiMI CAYNNNNRTG 2 cut(s) 412, 623
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
SphI GCATGC 1 cut(s) 281
SsiI CCGC 1 cut(s) 514
SspMI CTAG 2 cut(s) 33, 674
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 9, 59
StyI CCWWGG 1 cut(s) 401
TaaI ACNGT 3 cut(s) 128, 421, 635
TaiI ACGT 1 cut(s) 460
TaqI TCGA 4 cut(s) 42, 53, 87, 384
TatI WGTACW 2 cut(s) 416, 534
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 3 cut(s) 221, 306, 603
Tru9I TTAA 3 cut(s) 221, 306, 603
TscAI CASTG 1 cut(s) 640
TseI GCWGC 1 cut(s) 274
TspDTI ATGAA 4 cut(s) 91, 474, 554, 641
TspRI CASTG 1 cut(s) 640
VspI ATTAAT 1 cut(s) 603
XbaI TCTAGA 1 cut(s) 32
XceI RCATGY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 2 cut(s) 33, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.