MD16G1202400.v1.1

Guanine nucleotide-binding protein subunit gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012824
Physical Location & Seq
Forward (+)
18417249 .. 18420774
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1202400.v1.1.491

Sequence Viewer

Length: 318 bp
ATGCAATCGGATGGGTCGGAAACATTGAGCCCAACAACCCTACGGCTGCAGTCCTTGTCCGCCGCCGACACCAGAGGGAAGCACAGGATACATGCCGAGTTGAAGCGGCTCGAGCAAGAAACTAGATTCTTAGAGGAAGAACTGGAGCAACTTGAAAAGATGGATAAGGCATCATCTGCATGCAAGGAAATACTAAACAGTGCGGAAACAAGGCCTGATCCATTACTCCCAATAACACACGGCCCTCTTAATCCGTTCTGGGATCGATGGTTCGAAGGCCCCCAAGACTCAAAAGGTTGCAGATGCTGGATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
G-gamma

Protein Analysis

106

Amino Acids

12.05

Weight (kDa)

5.36

Isoelectric Point (pI)

68.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 33 - 105 4.1e-15 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22942
fragaria_vesca FvH4_4g01800
malus_domestica MD13G1202200.v1.1 MD16G1202400.v1.1
prunus_persica Prupe.1G024000_v2.0.a1 Prupe.1G024000_v2.0.a1
pyrus_communis pycom13g17560
rosa_chinensis RchiOBHm_Chr4g0388841
rosa_laevigata RLG00000010021
rosa_multiflora Rmu_sc0016023.1_g000005
rosa_roxburghii Rroxscaffold_5G00335120
rosa_rugosa Rorug03G0320300 Rorug03G0320400
rosa_samantha Rh4AG025000 Rh4BG018100 Rh4CG027900 Rh4DG018700
rosa_wichuraiana Rw4G001790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 60, 63, 106, 203
AclWI GGATC 2 cut(s) 212, 270
AgsI TTSAA 2 cut(s) 103, 155
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AlwI GGATC 2 cut(s) 212, 270
AlwNI CAGNNNCTG 1 cut(s) 306
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 3 cut(s) 212, 241, 277
ApeKI GCWGC 1 cut(s) 46
AspS9I GGNCC 2 cut(s) 242, 278
AsuII TTCGAA 1 cut(s) 273
AvaI CYCGRG 1 cut(s) 110
BanII GRGCYC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 33
BccI CCATC 3 cut(s) 5, 154, 261
BceAI ACGGC 2 cut(s) 59, 256
BciVI GTATCC 1 cut(s) 81
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 47
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 3 cut(s) 47, 63, 107
BlsI GCNGC 3 cut(s) 48, 64, 108
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 242, 278
BmiI GGNNCC 1 cut(s) 280
BmsI GCATC 2 cut(s) 179, 293
BpmI CTGGAG 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 273
Bsa29I ATCGAT 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 147
BseCI ATCGAT 1 cut(s) 265
BseGI GGATG 1 cut(s) 16
BseNI ACTGG 1 cut(s) 147
BseXI GCAGC 1 cut(s) 33
BshFI GGCC 3 cut(s) 214, 243, 279
BshVI ATCGAT 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 110
BsnI GGCC 3 cut(s) 214, 243, 279
BsoBI CYCGRG 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 217, 262
BspACI CCGC 4 cut(s) 60, 63, 106, 203
BspANI GGCC 3 cut(s) 214, 243, 279
BspDI ATCGAT 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 280
BspMAI CTGCAG 1 cut(s) 51
BspPI GGATC 2 cut(s) 212, 270
BspT104I TTCGAA 1 cut(s) 273
BsrI ACTGG 1 cut(s) 147
BssMI GATC 2 cut(s) 217, 262
Bst4CI ACNGT 1 cut(s) 200
BstAPI GCANNNNNTGC 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 2 cut(s) 220, 265
BstMBI GATC 2 cut(s) 217, 262
BstMWI GCNNNNNNNGC 2 cut(s) 112, 176
BstNSI RCATGY 2 cut(s) 95, 183
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 265
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 3 cut(s) 214, 243, 279
BsuTUI ATCGAT 1 cut(s) 265
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 242, 278
ClaI ATCGAT 1 cut(s) 265
CviAII CATG 2 cut(s) 92, 180
CviJI RGCY 6 cut(s) 30, 46, 109, 214, 243, 279
CviKI_1 RGCY 6 cut(s) 30, 46, 109, 214, 243, 279
DdeI CTNAG 1 cut(s) 130
DpnI GATC 2 cut(s) 219, 264
DpnII GATC 2 cut(s) 217, 262
EciI GGCGGA 1 cut(s) 49
Eco147I AGGCCT 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 32
FaeI CATG 2 cut(s) 95, 183
FaiI YATR 2 cut(s) 93, 181
FatI CATG 2 cut(s) 91, 179
Fnu4HI GCNGC 3 cut(s) 47, 63, 107
FokI GGATG 1 cut(s) 23
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 3 cut(s) 47, 63, 107
FspBI CTAG 1 cut(s) 123
GluI GCNGC 3 cut(s) 47, 63, 107
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 3 cut(s) 214, 243, 279
Hin1II CATG 2 cut(s) 95, 183
HinfI GANTC 2 cut(s) 126, 287
Hpy188I TCNGA 2 cut(s) 10, 19
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4V TGCA 5 cut(s) 4, 49, 179, 183, 300
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 176
HpyF3I CTNAG 1 cut(s) 130
Hsp92II CATG 2 cut(s) 95, 183
Kzo9I GATC 2 cut(s) 217, 262
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 6 cut(s) 70, 85, 128, 228, 244, 292
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 2 cut(s) 179, 293
MaeI CTAG 1 cut(s) 123
MalI GATC 2 cut(s) 219, 264
MboI GATC 2 cut(s) 217, 262
MboII GAAGA 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 3 cut(s) 68, 127, 255
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 178
MwoI GCNNNNNNNGC 2 cut(s) 112, 176
NdeII GATC 2 cut(s) 217, 262
NlaIII CATG 2 cut(s) 95, 183
NlaIV GGNNCC 1 cut(s) 280
NmeAIII GCCGAG 1 cut(s) 121
NspI RCATGY 2 cut(s) 95, 183
NspV TTCGAA 1 cut(s) 273
PaeI GCATGC 1 cut(s) 183
PaeR7I CTCGAG 1 cut(s) 110
PceI AGGCCT 1 cut(s) 214
PfeI GAWTC 1 cut(s) 126
PkrI GCNGC 3 cut(s) 48, 64, 108
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 2 cut(s) 242, 278
PspXI VCTCGAGB 1 cut(s) 110
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 306
RseI CAYNNNNRTG 1 cut(s) 178
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 3 cut(s) 47, 63, 107
Sau3AI GATC 2 cut(s) 217, 262
Sau96I GGNCC 2 cut(s) 242, 278
SchI GAGTC 1 cut(s) 281
SduI GDGCHC 1 cut(s) 32
SetI ASST 1 cut(s) 298
SfaNI GCATC 2 cut(s) 179, 293
SfcI CTRYAG 1 cut(s) 47
Sfr274I CTCGAG 1 cut(s) 110
SfuI TTCGAA 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SphI GCATGC 1 cut(s) 183
SseBI AGGCCT 1 cut(s) 214
SsiI CCGC 4 cut(s) 60, 63, 106, 203
SspMI CTAG 1 cut(s) 123
StuI AGGCCT 1 cut(s) 214
TaaI ACNGT 1 cut(s) 200
TaqI TCGA 3 cut(s) 111, 265, 273
TauI GCSGC 2 cut(s) 65, 109
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 205
TseI GCWGC 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 1 cut(s) 205
XceI RCATGY 2 cut(s) 95, 183
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.