Rh4AG025000

Guanine nucleotide-binding protein subunit gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012824
Physical Location & Seq
Forward (+)
4847235 .. 4850369
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG025000.1

Sequence Viewer

Length: 324 bp
ATGCAATCGGACGGGTCCGATTCCGTGAGCCAGAGTCCAACAACCCAAAGGCTGCAGTCTCTGTCAGCCTCGGATACTAGAGGGAAGCATCGGATACTGGCTGAGTTGAAACGGTTCGAGCAAGAAGCTAGATTCTTGGAGGAAGAGCTGGAACAACTTGAAAAGATAGACAAAGCCTCAGCTGCATGCAAGGAAATGCTGAGTAATGTAGAAACAAGGCCAGATCCACTACTCCCAGTAACACATGGCCCCCTTAATCCATTTTGGGACCGATGGTTTGAAGGCCCCAAAGACTCAAGTGGTTGCAAATGCTGGATACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
G-gamma

Protein Analysis

107

Amino Acids

12.16

Weight (kDa)

5.17

Isoelectric Point (pI)

63.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 35 - 107 1.4e-14 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22942
fragaria_vesca FvH4_4g01800
malus_domestica MD13G1202200.v1.1 MD16G1202400.v1.1
prunus_persica Prupe.1G024000_v2.0.a1 Prupe.1G024000_v2.0.a1
pyrus_communis pycom13g17560
rosa_chinensis RchiOBHm_Chr4g0388841
rosa_laevigata RLG00000010021
rosa_multiflora Rmu_sc0016023.1_g000005
rosa_roxburghii Rroxscaffold_5G00335120
rosa_rugosa Rorug03G0320300 Rorug03G0320400
rosa_samantha Rh4AG025000 Rh4BG018100 Rh4CG027900 Rh4DG018700
rosa_wichuraiana Rw4G001790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 218
AgsI TTSAA 3 cut(s) 109, 161, 281
AluBI AGCT 3 cut(s) 128, 148, 182
AluI AGCT 3 cut(s) 128, 148, 182
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 1 cut(s) 218
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 3 cut(s) 218, 247, 283
ApeKI GCWGC 2 cut(s) 52, 182
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 113
AspS9I GGNCC 4 cut(s) 15, 248, 268, 284
AvaII GGWCC 2 cut(s) 15, 268
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 39, 169
BccI CCATC 1 cut(s) 267
BciVI GTATCC 3 cut(s) 67, 87, 309
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 2 cut(s) 78, 129
BfmI CTRYAG 1 cut(s) 53
BfuI GTATCC 3 cut(s) 67, 87, 309
BisI GCNGC 2 cut(s) 53, 183
BlsI GCNGC 2 cut(s) 54, 184
Bme18I GGWCC 2 cut(s) 15, 268
BmgT120I GGNCC 4 cut(s) 15, 248, 268, 284
BmiI GGNNCC 4 cut(s) 16, 250, 269, 286
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 102, 236
BseDI CCNNGG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 93, 191, 192
BseNI ACTGG 2 cut(s) 102, 236
BseXI GCAGC 2 cut(s) 39, 169
BshFI GGCC 3 cut(s) 220, 249, 285
BslFI GGGAC 1 cut(s) 281
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 3 cut(s) 220, 249, 285
Bsp143I GATC 1 cut(s) 223
BspANI GGCC 3 cut(s) 220, 249, 285
BspCNI CTCAG 3 cut(s) 94, 191, 192
BspLI GGNNCC 4 cut(s) 16, 250, 269, 286
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 1 cut(s) 218
BspQI GCTCTTC 1 cut(s) 138
BsrI ACTGG 2 cut(s) 102, 236
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 1 cut(s) 223
Bst4CI ACNGT 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 102, 178, 200
BstKTI GATC 1 cut(s) 226
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 2 cut(s) 39, 169
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuI GTATCC 3 cut(s) 67, 87, 309
BsuRI GGCC 3 cut(s) 220, 249, 285
Cac8I GCNNGC 1 cut(s) 187
CaiI CAGNNNCTG 1 cut(s) 61
Cfr13I GGNCC 4 cut(s) 15, 248, 268, 284
CviAII CATG 2 cut(s) 186, 245
DdeI CTNAG 3 cut(s) 102, 178, 200
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 15, 268
EcoO109I RGGNCCY 1 cut(s) 284
FaeI CATG 2 cut(s) 189, 248
FaiI YATR 2 cut(s) 187, 246
FaqI GGGAC 1 cut(s) 281
FatI CATG 2 cut(s) 185, 244
Fnu4HI GCNGC 2 cut(s) 53, 183
Fsp4HI GCNGC 2 cut(s) 53, 183
FspBI CTAG 2 cut(s) 78, 129
GluI GCNGC 2 cut(s) 53, 183
HaeIII GGCC 3 cut(s) 220, 249, 285
Hin1II CATG 2 cut(s) 189, 248
HinfI GANTC 4 cut(s) 20, 34, 132, 293
Hpy188I TCNGA 4 cut(s) 10, 19, 73, 93
HpyAV CCTTC 1 cut(s) 275
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4V TGCA 5 cut(s) 4, 55, 185, 189, 306
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 3 cut(s) 102, 178, 200
Hsp92II CATG 2 cut(s) 189, 248
Kzo9I GATC 1 cut(s) 223
LguI GCTCTTC 1 cut(s) 138
LpnPI CCDG 6 cut(s) 44, 83, 134, 234, 249, 298
Lsp1109I GCAGC 2 cut(s) 39, 169
LweI GCATC 1 cut(s) 97
MaeI CTAG 2 cut(s) 78, 129
MaeIII GTNAC 1 cut(s) 238
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MboII GAAGA 1 cut(s) 155
MflI RGATCY 1 cut(s) 223
MlyI GAGTC 2 cut(s) 43, 287
MmeI TCCRAC 1 cut(s) 62
MnlI CCTC 4 cut(s) 74, 79, 133, 187
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 182
NdeII GATC 1 cut(s) 223
NlaIII CATG 2 cut(s) 189, 248
NlaIV GGNNCC 4 cut(s) 16, 250, 269, 286
NspI RCATGY 1 cut(s) 189
PaeI GCATGC 1 cut(s) 189
PciSI GCTCTTC 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 20, 132
PkrI GCNGC 2 cut(s) 54, 184
PleI GAGTC 2 cut(s) 42, 287
PpsI GAGTC 2 cut(s) 42, 287
PspN4I GGNNCC 4 cut(s) 16, 250, 269, 286
PspPI GGNCC 4 cut(s) 15, 248, 268, 284
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 61
PsuI RGATCY 1 cut(s) 223
PvuII CAGCTG 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 2 cut(s) 53, 183
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 4 cut(s) 15, 248, 268, 284
SchI GAGTC 2 cut(s) 43, 287
SetI ASST 3 cut(s) 130, 150, 184
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 2 cut(s) 15, 268
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
SphI GCATGC 1 cut(s) 189
SspMI CTAG 2 cut(s) 78, 129
TaaI ACNGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 117
TaqII GACCGA 1 cut(s) 285
TfiI GAWTC 2 cut(s) 20, 132
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseI GCWGC 2 cut(s) 52, 182
TspGWI ACGGA 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 15, 268
XceI RCATGY 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 2 cut(s) 78, 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.