Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0016517

Physical Location & Seq

Genomic Coordinates Forward (+)

24439116 .. 24440921

Gene Structure 1806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1235500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGCATGCCTTGCACTTCACCACCACTACATCACCACCCTCTCCCGCCCTTCTCCAAAATGCCACCCAAACTTACACAGCCACAACAAAAATCACATGCAAAATCCTCATCAACCCATCCAAAAAAACTCTAACCCATAAAACCCAAAAACCACCAAGGCAACCCTTGCCACCCCCAAACACTAAACCATTACCGCAAGTGAACCCAGCAAAACCAAATATTAACCTACCTCGCCTAAACCCTTTTCAAAAATTAGCAGCCTCACTCCTAGACGCAGGAGAAACATCACTAATAGAGCCACTAGAAAAGAAGCATGCTTTGCCCAAAAACATCGACCCGGCTGTCCAAATATCCGGGAATTTTGCGCCGGTTCAAGAGTGTCCGGTTCATCACGGTCTCGAGGTTGTGGGACAAGTCCCTGATTGCTTACGTGGTGTTTATGTCCGAAACGGCGCGAACCCTATGATTCCACCGTCGGGCGGACACCACTTGTTTGATGGGGACGGTATGATTCATGCCCTCACTCTCGGCTCGTCAAATCGAGCCAGCTACAGCTGTAGGTACACACGCACGAGCCGACTTGACCAAGAAGCCATGCTAGGCAGGTCCATTTTTCCCAAGCCGATTGGGGAGCTTCATGGTCACTCGGGTTTGGCTCGGCTTGGTTTGTTCATGGCTCGAGCAGCTATCGGCTTGGTAGACAGCTCACGTGGCACCGGCGTCGCTAATGCCGGCTTGGTTTATTTCAACGGACGATTATTAGCCATGTCGGAAGATGATCTCCCGTACAGTGTTAAGATCAAGGGCGACGGTGATCTGGAAACAATCGGAAGGTTCGATTTTAACGGTGGATTGGACCGACCCATGATTGCGCATCCTAAGGTGGACCCCCAAACGGGTGAGCTCCACGCGCTCAGTTACGACGTGGTGAAGAAGCCGCACCTCAAGTACTTCAGGTTTAGCACGTGCGGGAAAAAATCACGTGACGTGGACATCACGCTAGACCAACCTACCATGGTGCATGACTTTGCGATAACCCAAAATTATGTGGTGATTCCGGATCAGCAAGTGGTATTTAAGTTATCCAAAATGATTAAGGGCGGTTGGCCCGTGATCTACGACCGGAGCAAGACGTCTCGGTTTGGAATTTTACCAAAAGGGGACGTGGACGAATCGAGCATCTGCTGGATTGACGTGCCAGATTGTTTTTGCTTCCATTTGTGCAATTCATGGGAAGAGATTTCGGACGCAGGTGACCCGACTGTTGTTGTCATTGGGTCATGCATGGACCCACCGGATTCAATATTCAACGAGCAAGGGAATAACCCAATTCGTGCTGAGTTGACAGAGATCCGGATGAACTTGAGGACTCGTGAGTCAACCCGGCGGGTTCTCGTCCCGAGTTTGAATTTGGAAGTAGGACAGGTGAACAAACAAGTGGTAGGACAAAAGAGCAAGCATGTGTATATGGCTATAGCTGAGCCGTGGCCCAAGTGTTCGGGTATTGCTAAGGTGGATTCGGAAACCGGTTCGGTGAGTAAGTATATGTATGGGTCCGGTCGGTTTGGCGGAGAACCGCTGTACGTGCCCCGACAGAGAAATGGTGGCATGGCACACCACGGTGAAGACGATGACGAAGGGTTTATAATGGGGTTTGTGAGGGATGAAGTTGAGGAGAGTTCGGAGTTGGTGATATTGGAAGCTTCAAGCATGAAACAAGTAGCGTTGGTGAGATTGCCTGCTAGGGTTCCGTATGGATTCCATGGTACATTTGTTAGTGAACAAGATTTGAAGCTGCAAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009639 GO:0009687 GO:0009688 GO:0009987 GO:0010114 GO:0010436 GO:0016020 GO:0016053 GO:0016106 GO:0016114 GO:0016491 GO:0016701 GO:0016702 GO:0019752 GO:0031976 GO:0031984 GO:0032787 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045549 GO:0046165 GO:0046394 GO:0050896 GO:0051213 GO:0055035 GO:0055114 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

RPE65

Protein Analysis

602

Amino Acids

66.03

Weight (kDa)

7.63

Isoelectric Point (pI)

41.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	121 - 593	1.6e-124	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0016517)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24220
fragaria_vesca	FvH4_4g05900
malus_domestica	MD16G1235500.v1.1
prunus_persica	Prupe.1G061300_v2.0.a1
pyrus_communis	pycom16g19710
rosa_chinensis	RchiOBHm_Chr4g0397001
rosa_laevigata	RLG00000009460
rosa_multiflora	Rmu_sc0002963.1_g000015
rosa_roxburghii	Rroxscaffold_5G00341990
rosa_rugosa	Rorug03G0345900
rosa_samantha	Rh4BG072400 Rh4CG080700 Rh4DG069800
rosa_wichuraiana	Rw4G006100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1646
AarI	CACCTGC	1 cut(s)	1241
AasI	GACNNNNNNGTC	2 cut(s)	341, 1375
AatII	GACGTC	1 cut(s)	1136
Acc16I	TGCGCA	1 cut(s)	873
Acc36I	ACCTGC	2 cut(s)	594, 1241
AccB1I	GGYRCC	1 cut(s)	713
AccI	GTMKAC	1 cut(s)	699
AccII	CGCG	2 cut(s)	455, 911
AccIII	TCCGGA	2 cut(s)	1057, 1353
AciI	CCGC	9 cut(s)	45, 194, 480, 938, 969, 1101, 1387, 1569, 1577
AclWI	GGATC	2 cut(s)	1068, 1345
AcsI	RAATTY	3 cut(s)	358, 1146, 1408
AcuI	CTGAAG	1 cut(s)	937
AcvI	CACGTG	3 cut(s)	710, 966, 983
AcyI	GRCGYC	2 cut(s)	720, 1133
AfaI	GTAC	5 cut(s)	563, 788, 950, 1583, 1768
AfiI	CCNNNNNNNGG	4 cut(s)	476, 479, 895, 896
AgeI	ACCGGT	1 cut(s)	1526
AgsI	TTSAA	8 cut(s)	248, 374, 748, 1302, 1309, 1408, 1707, 1792
AjiI	CACGTC	4 cut(s)	925, 988, 1165, 1195
AleI	CACNNNNGTG	1 cut(s)	1620
Alw21I	GWGCWC	1 cut(s)	906
Alw26I	GTCTC	2 cut(s)	401, 1140
AlwI	GGATC	2 cut(s)	1068, 1345
Ama87I	CYCGRG	4 cut(s)	398, 646, 678, 1399
Aor13HI	TCCGGA	2 cut(s)	1057, 1353
AoxI	GGCC	2 cut(s)	1106, 1487
ApeKI	GCWGC	3 cut(s)	257, 683, 1795
ApoI	RAATTY	3 cut(s)	358, 1146, 1408
AsiGI	ACCGGT	1 cut(s)	1526
AspLEI	GCGC	4 cut(s)	367, 455, 874, 913
AspS9I	GGNCC	7 cut(s)	606, 856, 886, 1107, 1288, 1488, 1554
AsuC2I	CCSGG	3 cut(s)	338, 355, 1384
AvaI	CYCGRG	4 cut(s)	398, 646, 678, 1399
AvaII	GGWCC	5 cut(s)	606, 856, 886, 1288, 1554
AxyI	CCTNAGG	1 cut(s)	879
BaeGI	GKGCMC	1 cut(s)	1590
BaeI	ACNNNNGTAYC	2 cut(s)	1758, 1791
BanI	GGYRCC	1 cut(s)	713
BanII	GRGCYC	1 cut(s)	906
BauI	CACGAG	2 cut(s)	571, 1371
BbrPI	CACGTG	3 cut(s)	710, 966, 983
BbsI	GAAGAC	1 cut(s)	1632
Bbv12I	GWGCWC	1 cut(s)	906
BbvI	GCAGC	3 cut(s)	269, 695, 1782
BccI	CCATC	2 cut(s)	124, 491
BceAI	ACGGC	2 cut(s)	466, 1468
BcgI	CGANNNNNNTGC	2 cut(s)	703, 737
BcnI	CCSGG	3 cut(s)	338, 355, 1384
BcoDI	GTCTC	2 cut(s)	401, 1140
BfaI	CTAG	5 cut(s)	269, 302, 599, 1001, 1743
BfmI	CTRYAG	3 cut(s)	550, 556, 1473
BfuAI	ACCTGC	2 cut(s)	594, 1241
BisI	GCNGC	4 cut(s)	258, 684, 938, 1796
BlpI	GCTNAGC	1 cut(s)	1479
BlsI	GCNGC	4 cut(s)	259, 685, 939, 1797
BmcAI	AGTACT	1 cut(s)	950
Bme1390I	CCNGG	3 cut(s)	338, 355, 1384
Bme18I	GGWCC	5 cut(s)	606, 856, 886, 1288, 1554
BmeT110I	CYCGRG	4 cut(s)	398, 646, 678, 1399
BmgBI	CACGTC	4 cut(s)	925, 988, 1165, 1195
BmgT120I	GGNCC	7 cut(s)	606, 856, 886, 1107, 1288, 1488, 1554
BmiI	GGNNCC	5 cut(s)	715, 888, 1290, 1555, 1749
BmrFI	CCNGG	3 cut(s)	338, 355, 1384
BmsI	GCATC	2 cut(s)	883, 1188
BpiI	GAAGAC	1 cut(s)	1632
Bpu10I	CCTNAGC	1 cut(s)	1509
Bpu1102I	GCTNAGC	1 cut(s)	1479
BpuEI	CTTGAG	2 cut(s)	929, 1384
BpuMI	CCSGG	3 cut(s)	338, 355, 1384
BsaAI	YACGTR	5 cut(s)	431, 710, 966, 983, 1585
BsaHI	GRCGYC	2 cut(s)	720, 1133
BsaI	GGTCTC	1 cut(s)	401
BsaJI	CCNNGG	5 cut(s)	155, 1014, 1484, 1618, 1762
BsaWI	WCCGGW	7 cut(s)	382, 1057, 1122, 1294, 1353, 1526, 1556
Bsc4I	CCNNNNNNNGG	4 cut(s)	476, 479, 895, 896
Bse118I	RCCGGY	4 cut(s)	367, 716, 731, 1526
Bse21I	CCTNAGG	1 cut(s)	879
BseAI	TCCGGA	2 cut(s)	1057, 1353
BseDI	CCNNGG	5 cut(s)	155, 1014, 1484, 1618, 1762
BseGI	GGATG	4 cut(s)	116, 874, 1362, 1669
BseLI	CCNNNNNNNGG	4 cut(s)	476, 479, 895, 896
BseMII	CTCAG	3 cut(s)	928, 1329, 1470
BseRI	GAGGAG	1 cut(s)	1688
BseSI	GKGCMC	1 cut(s)	1590
BseXI	GCAGC	3 cut(s)	269, 695, 1782
BseYI	CCCAGC	1 cut(s)	205
Bsh1236I	CGCG	2 cut(s)	455, 911
Bsh1285I	CGRYCG	2 cut(s)	1123, 1561
BshFI	GGCC	2 cut(s)	1108, 1489
BshNI	GGYRCC	1 cut(s)	713
BshTI	ACCGGT	1 cut(s)	1526
BsiEI	CGRYCG	2 cut(s)	1123, 1561
BsiHKAI	GWGCWC	1 cut(s)	906
BsiHKCI	CYCGRG	4 cut(s)	398, 646, 678, 1399
BslFI	GGGAC	5 cut(s)	401, 423, 515, 1175, 1382
BslI	CCNNNNNNNGG	4 cut(s)	476, 479, 895, 896
BsmAI	GTCTC	2 cut(s)	401, 1140
BsmBI	CGTCTC	1 cut(s)	1140
BsmFI	GGGAC	5 cut(s)	401, 423, 515, 1175, 1382
BsnI	GGCC	2 cut(s)	1108, 1489
Bso31I	GGTCTC	1 cut(s)	401
BsoBI	CYCGRG	4 cut(s)	398, 646, 678, 1399
Bsp1286I	GDGCHC	2 cut(s)	906, 1590
Bsp13I	TCCGGA	2 cut(s)	1057, 1353
Bsp143I	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
Bsp1720I	GCTNAGC	1 cut(s)	1479
Bsp19I	CCATGG	2 cut(s)	1014, 1762
BspACI	CCGC	9 cut(s)	45, 194, 480, 938, 969, 1101, 1387, 1569, 1577
BspANI	GGCC	2 cut(s)	1108, 1489
BspCNI	CTCAG	3 cut(s)	927, 1330, 1471
BspEI	TCCGGA	2 cut(s)	1057, 1353
BspFNI	CGCG	2 cut(s)	455, 911
BspLI	GGNNCC	5 cut(s)	715, 888, 1290, 1555, 1749
BspMI	ACCTGC	2 cut(s)	594, 1241
BspPI	GGATC	2 cut(s)	1068, 1345
BspT107I	GGYRCC	1 cut(s)	713
BspTNI	GGTCTC	1 cut(s)	401
BsrFI	RCCGGY	4 cut(s)	367, 716, 731, 1526
BssAI	RCCGGY	4 cut(s)	367, 716, 731, 1526
BssECI	CCNNGG	5 cut(s)	155, 1014, 1484, 1618, 1762
BssMI	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
BssNI	GRCGYC	2 cut(s)	720, 1133
BssSI	CACGAG	2 cut(s)	571, 1371
BssT1I	CCWWGG	3 cut(s)	155, 1014, 1762
Bst2BI	CACGAG	2 cut(s)	571, 1371
Bst4CI	ACNGT	8 cut(s)	395, 474, 506, 791, 812, 848, 1264, 1622
Bst6I	CTCTTC	1 cut(s)	1230
BstACI	GRCGYC	2 cut(s)	720, 1133
BstAPI	GCANNNNNTGC	3 cut(s)	10, 166, 319
BstBAI	YACGTR	5 cut(s)	431, 710, 966, 983, 1585
BstC8I	GCNNGC	7 cut(s)	6, 315, 547, 733, 1457, 1740, 1800
BstDEI	CTNAG	5 cut(s)	879, 914, 1338, 1479, 1509
BstDSI	CCRYGG	4 cut(s)	1014, 1484, 1618, 1762
BstEII	GGTNACC	1 cut(s)	1253
BstF5I	GGATG	4 cut(s)	116, 874, 1362, 1669
BstFNI	CGCG	2 cut(s)	455, 911
BstHHI	GCGC	4 cut(s)	367, 455, 874, 913
BstKTI	GATC	6 cut(s)	781, 801, 817, 1063, 1116, 1353
BstMAI	GTCTC	2 cut(s)	401, 1140
BstMBI	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
BstMCI	CGRYCG	2 cut(s)	1123, 1561
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 166, 319, 683, 711, 910, 1585
BstNSI	RCATGY	4 cut(s)	8, 99, 317, 1463
BstPI	GGTNACC	1 cut(s)	1253
BstSCI	CCNGG	3 cut(s)	336, 353, 1382
BstSFI	CTRYAG	3 cut(s)	550, 556, 1473
BstSLI	GKGCMC	1 cut(s)	1590
BstUI	CGCG	2 cut(s)	455, 911
BstV1I	GCAGC	3 cut(s)	269, 695, 1782
BstV2I	GAAGAC	1 cut(s)	1632
BstX2I	RGATCY	1 cut(s)	1350
BstYI	RGATCY	1 cut(s)	1350
Bsu36I	CCTNAGG	1 cut(s)	879
BsuRI	GGCC	2 cut(s)	1108, 1489
BtgI	CCRYGG	4 cut(s)	1014, 1484, 1618, 1762
BtrI	CACGTC	4 cut(s)	925, 988, 1165, 1195
BtsCI	GGATG	4 cut(s)	116, 874, 1362, 1669
BtsIMutI	CAGTG	1 cut(s)	796
BveI	ACCTGC	2 cut(s)	594, 1241
Cac8I	GCNNGC	7 cut(s)	6, 315, 547, 733, 1457, 1740, 1800
CfoI	GCGC	4 cut(s)	367, 455, 874, 913
Cfr10I	RCCGGY	4 cut(s)	367, 716, 731, 1526
Cfr13I	GGNCC	7 cut(s)	606, 856, 886, 1107, 1288, 1488, 1554
CseI	GACGC	3 cut(s)	281, 709, 1256
Csp6I	GTAC	5 cut(s)	562, 787, 949, 1582, 1767
CspAI	ACCGGT	1 cut(s)	1526
CviQI	GTAC	5 cut(s)	562, 787, 949, 1582, 1767
DdeI	CTNAG	5 cut(s)	879, 914, 1338, 1479, 1509
DpnI	GATC	6 cut(s)	780, 800, 816, 1062, 1115, 1352
DpnII	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
DrdI	GACNNNNNNGTC	2 cut(s)	341, 1375
DseDI	GACNNNNNNGTC	2 cut(s)	341, 1375
Eam1104I	CTCTTC	1 cut(s)	1230
EarI	CTCTTC	1 cut(s)	1230
EciI	GGCGGA	2 cut(s)	495, 1584
Ecl136II	GAGCTC	1 cut(s)	904
Eco130I	CCWWGG	3 cut(s)	155, 1014, 1762
Eco24I	GRGCYC	1 cut(s)	906
Eco31I	GGTCTC	1 cut(s)	401
Eco47I	GGWCC	5 cut(s)	606, 856, 886, 1288, 1554
Eco53kI	GAGCTC	1 cut(s)	904
Eco57I	CTGAAG	1 cut(s)	937
Eco72I	CACGTG	3 cut(s)	710, 966, 983
Eco81I	CCTNAGG	1 cut(s)	879
Eco88I	CYCGRG	4 cut(s)	398, 646, 678, 1399
Eco91I	GGTNACC	1 cut(s)	1253
EcoICRI	GAGCTC	1 cut(s)	904
EcoO65I	GGTNACC	1 cut(s)	1253
EcoT14I	CCWWGG	3 cut(s)	155, 1014, 1762
EcoT22I	ATGCAT	2 cut(s)	6, 1286
EcoT38I	GRGCYC	1 cut(s)	906
ErhI	CCWWGG	3 cut(s)	155, 1014, 1762
Esp3I	CGTCTC	1 cut(s)	1140
FaqI	GGGAC	5 cut(s)	401, 423, 515, 1175, 1382
FauI	CCCGC	3 cut(s)	52, 962, 1380
FblI	GTMKAC	1 cut(s)	699
Fnu4HI	GCNGC	4 cut(s)	258, 684, 938, 1796
FokI	GGATG	4 cut(s)	103, 861, 1369, 1676
FriOI	GRGCYC	1 cut(s)	906
Fsp4HI	GCNGC	4 cut(s)	258, 684, 938, 1796
FspBI	CTAG	5 cut(s)	269, 302, 599, 1001, 1743
FspI	TGCGCA	1 cut(s)	873
GlaI	GCGC	4 cut(s)	366, 454, 873, 912
GluI	GCNGC	4 cut(s)	258, 684, 938, 1796
GsaI	CCCAGC	1 cut(s)	209
HaeIII	GGCC	2 cut(s)	1108, 1489
HgaI	GACGC	3 cut(s)	281, 709, 1256
HhaI	GCGC	4 cut(s)	367, 455, 874, 913
Hin1I	GRCGYC	2 cut(s)	720, 1133
Hin6I	GCGC	4 cut(s)	365, 453, 872, 911
HinP1I	GCGC	4 cut(s)	365, 453, 872, 911
HincII	GTYRAC	2 cut(s)	1344, 1380
HindII	GTYRAC	2 cut(s)	1344, 1380
HindIII	AAGCTT	2 cut(s)	1701, 1800
HinfI	GANTC	9 cut(s)	466, 511, 1054, 1172, 1298, 1369, 1376, 1517, 1758
Hpy188I	TCNGA	6 cut(s)	446, 772, 830, 1246, 1522, 1684
Hpy188III	TCNNGA	7 cut(s)	374, 398, 818, 1058, 1354, 1373, 1399
Hpy99I	CGWCG	4 cut(s)	478, 725, 812, 926
HpyAV	CCTTC	3 cut(s)	59, 825, 1631
HpyCH4III	ACNGT	8 cut(s)	395, 474, 506, 791, 812, 848, 1264, 1622
HpyCH4V	TGCA	7 cut(s)	4, 13, 99, 1021, 1224, 1284, 1798
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 166, 319, 683, 711, 910, 1585
HpyF3I	CTNAG	5 cut(s)	879, 914, 1338, 1479, 1509
Hsp92I	GRCGYC	2 cut(s)	720, 1133
HspAI	GCGC	4 cut(s)	365, 453, 872, 911
Kpn2I	TCCGGA	2 cut(s)	1057, 1353
KroI	GCCGGC	1 cut(s)	731
KroNI	GCCGGC	1 cut(s)	733
Kzo9I	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
LmnI	GCTCC	3 cut(s)	631, 909, 1125
Lsp1109I	GCAGC	3 cut(s)	269, 695, 1782
LweI	GCATC	2 cut(s)	883, 1188
MaeI	CTAG	5 cut(s)	269, 302, 599, 1001, 1743
MaeIII	GTNAC	4 cut(s)	641, 917, 983, 1253
MalI	GATC	6 cut(s)	780, 800, 816, 1062, 1115, 1352
MboI	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
MboII	GAAGA	4 cut(s)	785, 943, 1247, 1637
MflI	RGATCY	1 cut(s)	1350
MhlI	GDGCHC	2 cut(s)	906, 1590
MluCI	AATT	7 cut(s)	251, 358, 1042, 1146, 1225, 1329, 1408
MlyI	GAGTC	2 cut(s)	1363, 1385
MmeI	TCCRAC	1 cut(s)	750
Mph1103I	ATGCAT	2 cut(s)	6, 1286
MroI	TCCGGA	2 cut(s)	1057, 1353
MroNI	GCCGGC	1 cut(s)	731
MseI	TTAA	6 cut(s)	222, 795, 843, 1077, 1095, 1804
MslI	CAYNNNNRTG	1 cut(s)	1620
MspA1I	CMGCKG	2 cut(s)	555, 1579
MspR9I	CCNGG	3 cut(s)	338, 355, 1384
MvnI	CGCG	2 cut(s)	455, 911
MwoI	GCNNNNNNNGC	7 cut(s)	10, 166, 319, 683, 711, 910, 1585
NaeI	GCCGGC	1 cut(s)	733
NciI	CCSGG	3 cut(s)	338, 355, 1384
NcoI	CCATGG	2 cut(s)	1014, 1762
NdeII	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
NgoMIV	GCCGGC	1 cut(s)	731
NlaIV	GGNNCC	5 cut(s)	715, 888, 1290, 1555, 1749
NmeAIII	GCCGAG	2 cut(s)	507, 637
NmuCI	GTSAC	3 cut(s)	641, 983, 1253
NsbI	TGCGCA	1 cut(s)	873
NsiI	ATGCAT	2 cut(s)	6, 1286
NspI	RCATGY	4 cut(s)	8, 99, 317, 1463
OliI	CACNNNNGTG	1 cut(s)	1620
PaeI	GCATGC	2 cut(s)	8, 317
PaeR7I	CTCGAG	2 cut(s)	398, 678
PaqCI	CACCTGC	1 cut(s)	1241
PcsI	WCGNNNNNNNCGW	3 cut(s)	574, 834, 1626
PdiI	GCCGGC	1 cut(s)	733
PfeI	GAWTC	7 cut(s)	466, 511, 1054, 1172, 1298, 1517, 1758
PfoI	TCCNGGA	1 cut(s)	353
PinAI	ACCGGT	1 cut(s)	1526
PkrI	GCNGC	4 cut(s)	259, 685, 939, 1797
PleI	GAGTC	2 cut(s)	1363, 1384
PmaCI	CACGTG	3 cut(s)	710, 966, 983
PmlI	CACGTG	3 cut(s)	710, 966, 983
PpsI	GAGTC	2 cut(s)	1363, 1384
Ppu21I	YACGTR	5 cut(s)	431, 710, 966, 983, 1585
PsiI	TTATAA	1 cut(s)	1646
Psp124BI	GAGCTC	1 cut(s)	906
PspCI	CACGTG	3 cut(s)	710, 966, 983
PspEI	GGTNACC	1 cut(s)	1253
PspFI	CCCAGC	1 cut(s)	205
PspN4I	GGNNCC	5 cut(s)	715, 888, 1290, 1555, 1749
PspPI	GGNCC	7 cut(s)	606, 856, 886, 1107, 1288, 1488, 1554
PspXI	VCTCGAGB	1 cut(s)	678
PsuI	RGATCY	1 cut(s)	1350
PvuII	CAGCTG	1 cut(s)	555
RsaI	GTAC	5 cut(s)	563, 788, 950, 1583, 1768
RsaNI	GTAC	5 cut(s)	562, 787, 949, 1582, 1767
RseI	CAYNNNNRTG	1 cut(s)	1620
SacI	GAGCTC	1 cut(s)	906
SaqAI	TTAA	6 cut(s)	222, 795, 843, 1077, 1095, 1804
SatI	GCNGC	4 cut(s)	258, 684, 938, 1796
Sau3AI	GATC	6 cut(s)	778, 798, 814, 1060, 1113, 1350
Sau96I	GGNCC	7 cut(s)	606, 856, 886, 1107, 1288, 1488, 1554
ScaI	AGTACT	1 cut(s)	950
SchI	GAGTC	2 cut(s)	1363, 1385
ScrFI	CCNGG	3 cut(s)	338, 355, 1384
SduI	GDGCHC	2 cut(s)	906, 1590
SfaNI	GCATC	2 cut(s)	883, 1188
SfcI	CTRYAG	3 cut(s)	550, 556, 1473
Sfr274I	CTCGAG	2 cut(s)	398, 678
SgrAI	CRCCGGYG	1 cut(s)	716
SinI	GGWCC	5 cut(s)	606, 856, 886, 1288, 1554
SlaI	CTCGAG	2 cut(s)	398, 678
SmiMI	CAYNNNNRTG	1 cut(s)	1620
SmlI	CTYRAG	4 cut(s)	398, 678, 944, 1363
SmoI	CTYRAG	4 cut(s)	398, 678, 944, 1363
SphI	GCATGC	2 cut(s)	8, 317
Sse9I	AATT	7 cut(s)	251, 358, 1042, 1146, 1225, 1329, 1408
SsiI	CCGC	9 cut(s)	45, 194, 480, 938, 969, 1101, 1387, 1569, 1577
SspI	AATATT	2 cut(s)	220, 1305
SspMI	CTAG	5 cut(s)	269, 302, 599, 1001, 1743
SstI	GAGCTC	1 cut(s)	906
StyD4I	CCNGG	3 cut(s)	336, 353, 1382
StyI	CCWWGG	3 cut(s)	155, 1014, 1762
TaaI	ACNGT	8 cut(s)	395, 474, 506, 791, 812, 848, 1264, 1622
TaqI	TCGA	6 cut(s)	333, 399, 541, 679, 837, 1175
TaqII	GACCGA	1 cut(s)	873
TasI	AATT	7 cut(s)	251, 358, 1042, 1146, 1225, 1329, 1408
TatI	WGTACW	1 cut(s)	948
TauI	GCSGC	1 cut(s)	940
TfiI	GAWTC	7 cut(s)	466, 511, 1054, 1172, 1298, 1517, 1758
Tru1I	TTAA	6 cut(s)	222, 795, 843, 1077, 1095, 1804
Tru9I	TTAA	6 cut(s)	222, 795, 843, 1077, 1095, 1804
TscAI	CASTG	1 cut(s)	796
TseFI	GTSAC	3 cut(s)	641, 983, 1253
TseI	GCWGC	3 cut(s)	257, 683, 1795
Tsp45I	GTSAC	3 cut(s)	641, 983, 1253
TspDTI	ATGAA	8 cut(s)	377, 503, 626, 661, 1218, 1373, 1680, 1727
TspGWI	ACGGA	2 cut(s)	765, 1740
TspRI	CASTG	1 cut(s)	796
VpaK11BI	GGWCC	5 cut(s)	606, 856, 886, 1288, 1554
XapI	RAATTY	3 cut(s)	358, 1146, 1408
XceI	RCATGY	4 cut(s)	8, 99, 317, 1463
XcmI	CCANNNNNNNNNTGG	1 cut(s)	494
XhoI	CTCGAG	2 cut(s)	398, 678
XmiI	GTMKAC	1 cut(s)	699
XspI	CTAG	5 cut(s)	269, 302, 599, 1001, 1743
ZraI	GACGTC	1 cut(s)	1134
ZrmI	AGTACT	1 cut(s)	950
Zsp2I	ATGCAT	2 cut(s)	6, 1286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.