MD16G1249500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015199
Physical Location & Seq
Forward (+)
27872753 .. 27876763
4011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1249500.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGCGGAAGAACCCAAAACGTTGTCGTCCAGCTCGCAACCTTCTTCTACACCGAATTCCCTCGAAGAAACTCTGAAGAAATCGCAGGTTCCAGCACTCTCTGGGCTTCCATCTTTCCCAAATGGTGATTTTCAGATGTTACCAGTCATGTATCCTCTTCTCGTTCCTGGGTTAAATCCTTCGCAAGATCAGGAGCAAATGAACCGTGGAGCAGGCATTTATGCTGTTCCTGTTCACCCATTTATGGGGCCAGTTACAGGAGCTCCATTTAACACTCTTATTCCTCTTACCTACAACATACCCACAACTAGAGCAAGTCCAGAGGTTGGCACTGTAGGGGAGCAGCAAGGCCAAGAGGGACAACAACAACAACAACAACAACAACGGCAACAGCCTGGACCTCAGAGACAAGTTGTTGTTAGGAGATTTCAGATTGCATTTCAGCTTGATGTGTTGCTTATTATAAAGCTTGCAGCTGTAATATTTCTGTTCAACCAAGATGGATCTAGACAAAGGCTGGTTCTCCTTGTGATTTTCGCTTCAATTGTCTATTTATATCAAACTGGCTCGCTTACACCATTAGTACGATGGCTTTCTCAAGGCATGCATAGGGCAGCTGTGCCCCCCCATCCGCCCAGGGCTGCCAGGGCCGGGAATGTTCCTCCGAATGCAAGGCAGGGGGTTGACAATGCTGCTTTGGCAGATGGACAGCCCGGGGCTGGGAACGATATTCGGCCTGCAGATGAAGGAAATCCAGCAGCAGAGAATGAGAATGCCCCGGCAGCTGATGGAGCCAATGGTGGTCACCAGTGGTGGGGAATTGTTAAAGAGATTCAGATGATCGTTTTCGGTTTCATCACTTCACTTCTTCCGGGTTTTCATAATATGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

32.09

Weight (kDa)

5.96

Isoelectric Point (pI)

53.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29960
fragaria_vesca FvH4_4g07340
malus_domestica MD16G1249500.v1.1
prunus_persica Prupe.1G076300_v2.0.a1 Prupe.1G076300_v2.0.a1
pyrus_communis pycom16g20890
rosa_chinensis RchiOBHm_Chr4g0399711
rosa_laevigata RLG00000009245
rosa_multiflora Rmu_ssc0000118.1_g000010
rosa_roxburghii Rroxscaffold_5G00344650
rosa_rugosa Rorug04G0018500
rosa_samantha Rh4AG096000 Rh4BG093500 Rh4CG104800 Rh4DG088800
rosa_wichuraiana Rw4G007930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 326
AciI CCGC 2 cut(s) 5, 632
AclI AACGTT 1 cut(s) 20
AclWI GGATC 1 cut(s) 511
AcsI RAATTY 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 95
AfaI GTAC 1 cut(s) 585
AfiI CCNNNNNNNGG 8 cut(s) 244, 245, 257, 326, 651, 719, 720, 814
AgsI TTSAA 2 cut(s) 493, 543
AjnI CCWGG 4 cut(s) 166, 394, 635, 644
AluBI AGCT 7 cut(s) 33, 263, 445, 469, 476, 617, 785
AluI AGCT 7 cut(s) 33, 263, 445, 469, 476, 617, 785
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 1 cut(s) 511
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 4 cut(s) 248, 349, 648, 734
ApeKI GCWGC 7 cut(s) 343, 473, 614, 641, 692, 758, 782
ApoI RAATTY 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 248, 398, 648
AsuC2I CCSGG 5 cut(s) 652, 714, 715, 779, 873
AsuHPI GGTGA 3 cut(s) 137, 227, 797
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 624
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 7 cut(s) 355, 485, 626, 628, 679, 770, 794
BccI CCATC 6 cut(s) 118, 494, 582, 636, 698, 782
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 4 cut(s) 168, 396, 637, 646
BciVI GTATCC 1 cut(s) 162
BcnI CCSGG 5 cut(s) 652, 714, 715, 779, 873
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 309, 507
BfmI CTRYAG 2 cut(s) 333, 738
BfuAI ACCTGC 1 cut(s) 76
BfuI GTATCC 1 cut(s) 162
BisI GCNGC 7 cut(s) 344, 474, 615, 642, 693, 759, 783
BlsI GCNGC 7 cut(s) 345, 475, 616, 643, 694, 760, 784
Bme1390I CCNGG 9 cut(s) 168, 396, 637, 646, 652, 714, 715, 779, 873
Bme18I GGWCC 1 cut(s) 398
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 3 cut(s) 248, 398, 648
BmiI GGNNCC 3 cut(s) 90, 249, 793
BmrFI CCNGG 9 cut(s) 168, 396, 637, 646, 652, 714, 715, 779, 873
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 5 cut(s) 652, 714, 715, 779, 873
BsaJI CCNNGG 8 cut(s) 167, 205, 635, 636, 645, 713, 714, 777
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 8 cut(s) 244, 245, 257, 326, 651, 719, 720, 814
Bse1I ACTGG 4 cut(s) 143, 251, 568, 808
BseBI CCWGG 4 cut(s) 168, 396, 637, 646
BseDI CCNNGG 8 cut(s) 167, 205, 635, 636, 645, 713, 714, 777
BseGI GGATG 1 cut(s) 628
BseLI CCNNNNNNNGG 8 cut(s) 244, 245, 257, 326, 651, 719, 720, 814
BseMII CTCAG 1 cut(s) 416
BseNI ACTGG 4 cut(s) 143, 251, 568, 808
BseSI GKGCMC 1 cut(s) 624
BseXI GCAGC 7 cut(s) 355, 485, 626, 628, 679, 770, 794
BseYI CCCAGC 1 cut(s) 719
BshFI GGCC 4 cut(s) 250, 351, 650, 736
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 4 cut(s) 651, 714, 779, 872
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 8 cut(s) 244, 245, 257, 326, 651, 719, 720, 814
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 372
BsmI GAATGC 2 cut(s) 673, 778
BsnI GGCC 4 cut(s) 250, 351, 650, 736
BsoBI CYCGRG 1 cut(s) 713
Bsp1286I GDGCHC 2 cut(s) 265, 624
Bsp143I GATC 3 cut(s) 187, 503, 840
BspACI CCGC 2 cut(s) 5, 632
BspANI GGCC 4 cut(s) 250, 351, 650, 736
BspCNI CTCAG 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 90, 249, 793
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 1 cut(s) 511
BsrI ACTGG 4 cut(s) 143, 251, 568, 808
BssECI CCNNGG 8 cut(s) 167, 205, 635, 636, 645, 713, 714, 777
BssMI GATC 3 cut(s) 187, 503, 840
Bst2UI CCWGG 4 cut(s) 168, 396, 637, 646
Bst4CI ACNGT 2 cut(s) 206, 334
Bst6I CTCTTC 1 cut(s) 162
BstC8I GCNNGC 6 cut(s) 35, 214, 471, 569, 605, 738
BstDEI CTNAG 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 803
BstF5I GGATG 1 cut(s) 628
BstKTI GATC 3 cut(s) 190, 506, 843
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 3 cut(s) 187, 503, 840
BstMWI GCNNNNNNNGC 4 cut(s) 647, 698, 782, 791
BstNI CCWGG 4 cut(s) 168, 396, 637, 646
BstNSI RCATGY 1 cut(s) 607
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 9 cut(s) 166, 394, 635, 644, 650, 712, 713, 777, 871
BstSFI CTRYAG 2 cut(s) 333, 738
BstSLI GKGCMC 1 cut(s) 624
BstV1I GCAGC 7 cut(s) 355, 485, 626, 628, 679, 770, 794
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 4 cut(s) 250, 351, 650, 736
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 2 cut(s) 330, 815
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 6 cut(s) 35, 214, 471, 569, 605, 738
Cfr13I GGNCC 3 cut(s) 248, 398, 648
Cfr9I CCCGGG 1 cut(s) 713
Csp6I GTAC 1 cut(s) 584
CviAII CATG 2 cut(s) 148, 604
CviQI GTAC 1 cut(s) 584
DdeI CTNAG 1 cut(s) 402
DpnI GATC 3 cut(s) 189, 505, 842
DpnII GATC 3 cut(s) 187, 503, 840
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
EciI GGCGGA 2 cut(s) 20, 621
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 95
Eco88I CYCGRG 1 cut(s) 713
Eco91I GGTNACC 1 cut(s) 803
EcoICRI GAGCTC 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 803
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 4 cut(s) 166, 394, 635, 644
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 265
FaeI CATG 2 cut(s) 151, 607
FaqI GGGAC 1 cut(s) 372
FatI CATG 2 cut(s) 147, 603
Fnu4HI GCNGC 7 cut(s) 344, 474, 615, 642, 693, 759, 783
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 7 cut(s) 344, 474, 615, 642, 693, 759, 783
FspBI CTAG 2 cut(s) 309, 507
GluI GCNGC 7 cut(s) 344, 474, 615, 642, 693, 759, 783
GsaI CCCAGC 1 cut(s) 723
HaeIII GGCC 4 cut(s) 250, 351, 650, 736
HapII CCGG 4 cut(s) 651, 714, 779, 872
Hin1II CATG 2 cut(s) 151, 607
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 1 cut(s) 832
HpaII CCGG 4 cut(s) 651, 714, 779, 872
HphI GGTGA 3 cut(s) 137, 227, 797
Hpy166II GTNNAC 2 cut(s) 235, 685
Hpy188I TCNGA 6 cut(s) 75, 135, 405, 432, 666, 837
Hpy188III TCNNGA 3 cut(s) 191, 320, 507
Hpy8I GTNNAC 2 cut(s) 235, 685
HpyAV CCTTC 3 cut(s) 51, 189, 740
HpyCH4III ACNGT 2 cut(s) 206, 334
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 5 cut(s) 437, 473, 607, 671, 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 647, 698, 782, 791
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 2 cut(s) 151, 607
Kzo9I GATC 3 cut(s) 187, 503, 840
LmnI GCTCC 6 cut(s) 193, 209, 260, 268, 340, 791
Lsp1109I GCAGC 7 cut(s) 355, 485, 626, 628, 679, 770, 794
MaeI CTAG 2 cut(s) 309, 507
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 3 cut(s) 138, 253, 803
MalI GATC 3 cut(s) 189, 505, 842
MboI GATC 3 cut(s) 187, 503, 840
MboII GAAGA 6 cut(s) 20, 36, 77, 88, 149, 860
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 2 cut(s) 265, 624
MluCI AATT 3 cut(s) 55, 543, 819
MnlI CCTC 7 cut(s) 71, 165, 294, 316, 349, 411, 672
Mph1103I ATGCAT 1 cut(s) 609
MseI TTAA 3 cut(s) 173, 270, 825
MspA1I CMGCKG 3 cut(s) 476, 617, 785
MspI CCGG 4 cut(s) 651, 714, 779, 872
MspR9I CCNGG 9 cut(s) 168, 396, 637, 646, 652, 714, 715, 779, 873
MunI CAATTG 1 cut(s) 543
Mva1269I GAATGC 2 cut(s) 673, 778
MvaI CCWGG 4 cut(s) 168, 396, 637, 646
MwoI GCNNNNNNNGC 4 cut(s) 647, 698, 782, 791
NciI CCSGG 5 cut(s) 652, 714, 715, 779, 873
NdeII GATC 3 cut(s) 187, 503, 840
NlaIII CATG 2 cut(s) 151, 607
NlaIV GGNNCC 3 cut(s) 90, 249, 793
NmuCI GTSAC 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 1 cut(s) 607
PaeI GCATGC 1 cut(s) 607
PasI CCCWGGG 1 cut(s) 636
PctI GAATGC 2 cut(s) 673, 778
PfeI GAWTC 1 cut(s) 832
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 7 cut(s) 345, 475, 616, 643, 694, 760, 784
PsiI TTATAA 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 265
Psp1406I AACGTT 1 cut(s) 20
Psp6I CCWGG 4 cut(s) 166, 394, 635, 644
PspEI GGTNACC 1 cut(s) 803
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 4 cut(s) 166, 394, 635, 644
PspN4I GGNNCC 3 cut(s) 90, 249, 793
PspPI GGNCC 3 cut(s) 248, 398, 648
PstI CTGCAG 1 cut(s) 742
PsuI RGATCY 1 cut(s) 503
PvuII CAGCTG 3 cut(s) 476, 617, 785
RsaI GTAC 1 cut(s) 585
RsaNI GTAC 1 cut(s) 584
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 3 cut(s) 173, 270, 825
SatI GCNGC 7 cut(s) 344, 474, 615, 642, 693, 759, 783
Sau3AI GATC 3 cut(s) 187, 503, 840
Sau96I GGNCC 3 cut(s) 248, 398, 648
ScrFI CCNGG 9 cut(s) 168, 396, 637, 646, 652, 714, 715, 779, 873
SduI GDGCHC 2 cut(s) 265, 624
SfcI CTRYAG 2 cut(s) 333, 738
SinI GGWCC 1 cut(s) 398
SmaI CCCGGG 1 cut(s) 715
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
SphI GCATGC 1 cut(s) 607
Sse9I AATT 3 cut(s) 55, 543, 819
SsiI CCGC 2 cut(s) 5, 632
SspI AATATT 1 cut(s) 483
SspMI CTAG 2 cut(s) 309, 507
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 9 cut(s) 166, 394, 635, 644, 650, 712, 713, 777, 871
TaaI ACNGT 2 cut(s) 206, 334
TaiI ACGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 63
TasI AATT 3 cut(s) 55, 543, 819
TfiI GAWTC 1 cut(s) 832
Tru1I TTAA 3 cut(s) 173, 270, 825
Tru9I TTAA 3 cut(s) 173, 270, 825
TscAI CASTG 2 cut(s) 337, 815
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 7 cut(s) 343, 473, 614, 641, 692, 758, 782
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 4 cut(s) 215, 759, 844, 869
TspMI CCCGGG 1 cut(s) 713
TspRI CASTG 2 cut(s) 337, 815
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 1 cut(s) 55
XbaI TCTAGA 1 cut(s) 506
XceI RCATGY 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 1 cut(s) 585
XmaI CCCGGG 1 cut(s) 713
XspI CTAG 2 cut(s) 309, 507
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.