MD16G1250700.v1.1

Uncharacterized ACR, COG1399

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013051
Physical Location & Seq
Reverse (-)
28050330 .. 28052156
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1250700.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGCAGAAGTTGGTCATCTGTTATCAGCAAGGCATATTAAGCTTCTCTCTGTAACTCCGGCAAACCCCAAATTCTCCGCTTCGGTGTCTCGTTCTTTCACAATCAGAGCTTCTTCCAAAAGGAACTCTCTCTCTCTGGGTAACAAAAAGAACAGCAGGAGCAGTAGCCCACGGAGTGTGATCAGATTATCAACAGCAGATGGGAAATGGCATGGGAATTGGAGCTGTGATTACATTCTCTCCCTCCAAGACCTGCGTTTGGATGATTTGATCGAAGATGAAAATACACATGACGCTCAAATTTCGGTTAATCTCTGCGTCCACAAGCATGCTAGCTTTGGCTTCTCCGTTGATGGAAGGATATTAACATCATTCAACAGAAAATGTAGCAACTGCTCTTCTACCTACTGCAGAAAGATTGACACACAATTCAACGTGTGGGTTCTGTCGTCAAGCAGAGACGAACACACGGTTATCTACGTGAAGCCTGGAAATGAAGCAGAACTTGATTCACTCATACAGGACACGATACGGCTTGCAACCTCCATTAAAGATACTTGTTCAGAGCTGTGTGAGAAATCATATCCCACCGTGCAGTATATTGGTGGAAAAAGCACTGCTTCTGTTGATAAGAGATGGTCTAGGCTTTTGGAGCTAAAGAATATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF177

Protein Analysis

224

Amino Acids

24.97

Weight (kDa)

9.08

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YceD PF02620 117 - 220 1.3e-08 Large ribosomal RNA subunit accumulation protein YceD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 261
AciI CCGC 1 cut(s) 78
AcsI RAATTY 2 cut(s) 71, 300
AdeI CACNNNGTG 1 cut(s) 176
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 2 cut(s) 376, 433
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 6 cut(s) 43, 110, 225, 336, 568, 655
AluI AGCT 6 cut(s) 43, 110, 225, 336, 568, 655
Alw26I GTCTC 2 cut(s) 93, 453
ApoI RAATTY 2 cut(s) 71, 300
AsuNHI GCTAGC 1 cut(s) 332
BccI CCATC 3 cut(s) 194, 347, 630
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 1 cut(s) 489
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 93, 453
BfaI CTAG 2 cut(s) 333, 642
BfmI CTRYAG 1 cut(s) 409
BfuAI ACCTGC 1 cut(s) 261
Bme1390I CCNGG 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 489
BmtI GCTAGC 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 614
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 93, 453
BsmBI CGTCTC 1 cut(s) 453
Bsp143I GATC 2 cut(s) 180, 270
BspACI CCGC 1 cut(s) 78
BspMAI CTGCAG 1 cut(s) 413
BspMI ACCTGC 1 cut(s) 261
BspOI GCTAGC 1 cut(s) 336
BspQI GCTCTTC 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 2 cut(s) 180, 270
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 2 cut(s) 472, 592
Bst6I CTCTTC 1 cut(s) 403
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 330, 334, 537
BstDSI CCRYGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 2 cut(s) 183, 273
BstMAI GTCTC 2 cut(s) 93, 453
BstMBI GATC 2 cut(s) 180, 270
BstMWI GCNNNNNNNGC 2 cut(s) 40, 652
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 409
BtgI CCRYGG 1 cut(s) 170
BtsCI GGATG 1 cut(s) 268
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 1 cut(s) 615
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 3 cut(s) 330, 334, 537
CseI GACGC 2 cut(s) 302, 307
CviAII CATG 3 cut(s) 212, 290, 329
DpnI GATC 2 cut(s) 182, 272
DpnII GATC 2 cut(s) 180, 270
DraIII CACNNNGTG 1 cut(s) 176
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 487
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 3 cut(s) 215, 293, 332
FaiI YATR 8 cut(s) 36, 213, 291, 330, 518, 583, 600, 665
FalI AAGNNNNNCTT 4 cut(s) 489, 521, 604, 636
FatI CATG 3 cut(s) 211, 289, 328
FbaI TGATCA 1 cut(s) 180
FokI GGATG 1 cut(s) 275
FspBI CTAG 2 cut(s) 333, 642
HapII CCGG 1 cut(s) 59
HgaI GACGC 2 cut(s) 302, 307
Hin1II CATG 3 cut(s) 215, 293, 332
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 1 cut(s) 509
HpaII CCGG 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 3 cut(s) 107, 185, 565
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 2 cut(s) 472, 592
HpyCH4IV ACGT 2 cut(s) 435, 480
HpyCH4V TGCA 4 cut(s) 411, 539, 595, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 652
HpySE526I ACGT 2 cut(s) 435, 480
Hsp92II CATG 3 cut(s) 215, 293, 332
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 270
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 3 cut(s) 159, 222, 652
LpnPI CCDG 7 cut(s) 72, 122, 142, 266, 474, 501, 506
MaeI CTAG 2 cut(s) 333, 642
MaeII ACGT 2 cut(s) 435, 480
MaeIII GTNAC 2 cut(s) 52, 140
MalI GATC 2 cut(s) 182, 272
MboI GATC 2 cut(s) 180, 270
MboII GAAGA 3 cut(s) 105, 287, 390
MluCI AATT 4 cut(s) 71, 217, 300, 428
MnlI CCTC 2 cut(s) 254, 553
MseI TTAA 4 cut(s) 39, 309, 365, 549
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 40, 652
NdeII GATC 2 cut(s) 180, 270
NheI GCTAGC 1 cut(s) 332
NlaIII CATG 3 cut(s) 215, 293, 332
NspI RCATGY 1 cut(s) 332
PaeI GCATGC 1 cut(s) 332
PciSI GCTCTTC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 481
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PstI CTGCAG 1 cut(s) 413
RseI CAYNNNNRTG 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 403
SaqAI TTAA 4 cut(s) 39, 309, 365, 549
Sau3AI GATC 2 cut(s) 180, 270
ScrFI CCNGG 1 cut(s) 489
SfcI CTRYAG 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 327
SphI GCATGC 1 cut(s) 332
Sse9I AATT 4 cut(s) 71, 217, 300, 428
SsiI CCGC 1 cut(s) 78
SspMI CTAG 2 cut(s) 333, 642
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 2 cut(s) 472, 592
TaiI ACGT 2 cut(s) 438, 483
TaqI TCGA 1 cut(s) 273
TasI AATT 4 cut(s) 71, 217, 300, 428
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 4 cut(s) 39, 309, 365, 549
Tru9I TTAA 4 cut(s) 39, 309, 365, 549
TscAI CASTG 1 cut(s) 622
TspDTI ATGAA 2 cut(s) 294, 510
TspGWI ACGGA 2 cut(s) 187, 337
TspRI CASTG 1 cut(s) 622
XapI RAATTY 2 cut(s) 71, 300
XceI RCATGY 1 cut(s) 332
XspI CTAG 2 cut(s) 333, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.