Rh4DG091100

Uncharacterized ACR, COG1399

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013051
Physical Location & Seq
Reverse (-)
13733767 .. 13736553
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG091100.1

Sequence Viewer

Length: 744 bp
ATGGTTTTCAGAGAAATGGCAGAAGTTGCTAATTTGGTATCAGCAAGAAATATTAAGCTTCCTGGTATAAACCCAGCAAACGCCAGATCCTCCAAGTTGGTTTATTCTCAGAGTTTCAAAATCAGAGCTTCTTCAAAAAGAAACGACTTCTCTCTGATTAACAAAAAGAACAGCAGGCGCAGTGTGATCAGAATATCAACAGCAGATGGGAGATGGCATGGGAATTGGGGTTGTGATTACCTTCTGTCCCTCCAAGACCTGCGTTTGGGAGATTTGGTCGAAGAAGATGATGGAAATAAAAAGGATGCACAAGTTTCAGTCAACCTCTCCGTCCACAAGCATGCTAGTTTTGGCTTCTCTGTTGATGGAAGGATCTTAACATCATTCACCAGAAAATGTAGCAACTGCTCCTCTCCATACTGCAGAAAGATTGACACACAGTTTAATGTATGGGTTCTGTCATCAAGCAGAGATGACAACACAATTCAACTACCAGAAATCGGTGGCGACGATCCATCATTAACGGAACTTGATTCACTGGTACAAGACTCAATACGGCTCGCCACCTCCATAAAGGATACTTGTTCAGAGCTATGTGAAAAATCTTATCCAACCGTGCAGTATATTGGTGGAGAGAGCAGGGCTTCTATTGATAAAAGTTCGAACAAAGTTGGATCAAACACCTCACCAAATTGGAAGAATTATACTCTATCATCAGTCTTCAGTAAAAGGTTACTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF177

Protein Analysis

247

Amino Acids

27.52

Weight (kDa)

8.99

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YceD PF02620 121 - 213 2.1e-06 Large ribosomal RNA subunit accumulation protein YceD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 267
AclWI GGATC 4 cut(s) 81, 380, 506, 682
AcuI CTGAAG 1 cut(s) 706
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 2 cut(s) 265, 500
AgsI TTSAA 3 cut(s) 118, 135, 488
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 3 cut(s) 58, 128, 592
AluI AGCT 3 cut(s) 58, 128, 592
AlwI GGATC 4 cut(s) 81, 380, 506, 682
AspLEI GCGC 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 379, 678
AsuII TTCGAA 1 cut(s) 662
BbsI GAAGAC 1 cut(s) 712
BccI CCATC 5 cut(s) 200, 207, 284, 359, 523
BceAI ACGGC 1 cut(s) 572
BciT130I CCWGG 1 cut(s) 63
BciVI GTATCC 1 cut(s) 571
BclI TGATCA 1 cut(s) 186
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 421
BfuAI ACCTGC 1 cut(s) 267
BfuI GTATCC 1 cut(s) 571
Bme1390I CCNGG 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 712
Bpu14I TTCGAA 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 500
Bse1I ACTGG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 2 cut(s) 265, 500
BseMII CTCAG 1 cut(s) 122
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 400
BseYI CCCAGC 1 cut(s) 73
BsgI GTGCAG 1 cut(s) 638
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 265, 500
BsmFI GGGAC 1 cut(s) 232
Bsp119I TTCGAA 1 cut(s) 662
Bsp143I GATC 5 cut(s) 86, 186, 372, 511, 674
BspCNI CTCAG 1 cut(s) 121
BspMAI CTGCAG 1 cut(s) 425
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 4 cut(s) 81, 380, 506, 682
BspT104I TTCGAA 1 cut(s) 662
BsrI ACTGG 1 cut(s) 543
BssMI GATC 5 cut(s) 86, 186, 372, 511, 674
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 441, 616
BstAPI GCANNNNNTGC 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 3 cut(s) 176, 342, 561
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 5 cut(s) 89, 189, 375, 514, 677
BstMBI GATC 5 cut(s) 86, 186, 372, 511, 674
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 712
BstX2I RGATCY 2 cut(s) 86, 372
BstYI RGATCY 2 cut(s) 86, 372
BsuI GTATCC 1 cut(s) 571
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 187, 536
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 3 cut(s) 176, 342, 561
CfoI GCGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 542
CviAII CATG 2 cut(s) 218, 341
CviJI RGCY 6 cut(s) 58, 128, 354, 559, 592, 644
CviKI_1 RGCY 6 cut(s) 58, 128, 354, 559, 592, 644
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 1 cut(s) 108
DpnI GATC 5 cut(s) 88, 188, 374, 513, 676
DpnII GATC 5 cut(s) 86, 186, 372, 511, 674
Eco57I CTGAAG 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 61
FaeI CATG 2 cut(s) 221, 344
FaiI YATR 9 cut(s) 68, 219, 342, 418, 451, 572, 595, 624, 705
FaqI GGGAC 1 cut(s) 232
FatI CATG 2 cut(s) 217, 340
FbaI TGATCA 1 cut(s) 186
FokI GGATG 1 cut(s) 317
FspBI CTAG 1 cut(s) 345
GlaI GCGC 1 cut(s) 179
GsaI CCCAGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 180
Hin1II CATG 2 cut(s) 221, 344
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 2 cut(s) 533, 548
HphI GGTGA 2 cut(s) 379, 678
Hpy166II GTNNAC 2 cut(s) 322, 334
Hpy188I TCNGA 6 cut(s) 11, 111, 125, 156, 191, 589
Hpy8I GTNNAC 2 cut(s) 322, 334
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 2 cut(s) 251, 363
HpyCH4III ACNGT 2 cut(s) 441, 616
HpyCH4V TGCA 3 cut(s) 308, 423, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 2 cut(s) 221, 344
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 5 cut(s) 86, 186, 372, 511, 674
LmnI GCTCC 1 cut(s) 413
LweI GCATC 1 cut(s) 295
MaeI CTAG 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 732
MalI GATC 5 cut(s) 88, 188, 374, 513, 676
MboI GATC 5 cut(s) 86, 186, 372, 511, 674
MboII GAAGA 5 cut(s) 123, 293, 296, 709, 712
MflI RGATCY 2 cut(s) 86, 372
MluCI AATT 5 cut(s) 31, 223, 483, 691, 700
MlyI GAGTC 1 cut(s) 542
MmeI TCCRAC 2 cut(s) 635, 652
MnlI CCTC 6 cut(s) 100, 260, 335, 421, 577, 694
MseI TTAA 5 cut(s) 54, 159, 377, 444, 521
MslI CAYNNNNRTG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 5 cut(s) 86, 186, 372, 511, 674
NlaIII CATG 2 cut(s) 221, 344
NspI RCATGY 1 cut(s) 344
NspV TTCGAA 1 cut(s) 662
PaeI GCATGC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 1 cut(s) 533
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 73
PspGI CCWGG 1 cut(s) 61
PstI CTGCAG 1 cut(s) 425
PsuI RGATCY 2 cut(s) 86, 372
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 5 cut(s) 54, 159, 377, 444, 521
Sau3AI GATC 5 cut(s) 86, 186, 372, 511, 674
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 63
SetI ASST 9 cut(s) 60, 130, 243, 261, 327, 569, 594, 686, 734
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 421
SfuI TTCGAA 1 cut(s) 662
SmiMI CAYNNNNRTG 1 cut(s) 339
SphI GCATGC 1 cut(s) 344
Sse9I AATT 5 cut(s) 31, 223, 483, 691, 700
SspI AATATT 1 cut(s) 52
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 61
TaaI ACNGT 2 cut(s) 441, 616
TaqI TCGA 3 cut(s) 279, 662, 738
TasI AATT 5 cut(s) 31, 223, 483, 691, 700
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 5 cut(s) 54, 159, 377, 444, 521
Tru9I TTAA 5 cut(s) 54, 159, 377, 444, 521
TscAI CASTG 2 cut(s) 187, 543
TspGWI ACGGA 2 cut(s) 319, 539
TspRI CASTG 2 cut(s) 187, 543
XceI RCATGY 1 cut(s) 344
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.