RLG00000009235

Uncharacterized ACR, COG1399

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013051
Physical Location & Seq
Forward (+)
49512434 .. 49515027
2594 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009235

Sequence Viewer

Length: 717 bp
ATGGCAGAAGTTACTAATTTGGTATCAGCAAGAAATATTAAGCTTCCTGGTATAAACCCAGCAAACCCCAGATCCTCCAAGTTGGTTTATTCTCAGAGTTTCAGAATCAGAGCTTCTTCAAAAAGAAACGACTTCTCTCTGATTAACAAAAAGAACAGCAGGCGCAGTGTGATCAGAATATCAACAGCAAATGGGAGATGGCATGGAAATTGGGGTTGTGATTACCTTCTGTCCCTTCAAGACCTGCGTTTGGGAGATTTGGTCGAAGAAGATGATGGAAATAAAAAGGATGGACAAGTTTCAGTCAACCTCTCCGTCCACAAGCATGCTAGTTTTGGCTTCTCTGTTGATGGAAGGATCTTAACATCATTCACCAGAAAATGTAGTAACTGCTCCTCTCCATACTGCAGAAAGATTGACACACAGTTTAATGTATGGGTTCTGTCATCGAGCAGAGATGACAACACGATTCAACTACCAGAAATCGATAGTATCTTAACACAGCTGCTTCAACAGGTGATCTATGTGAAGCCTGGAAATGAAGCTGAACTTGATTCACTGGTACAAGACTCAATACGGCTCGCCACCTCCATAAAAGATACTTGTTCAGAGCTATGTGAAAAATCTTATCCAACTGTGCAGTATATTGGTGGAGAGAGCAGGGCTTCTATTGATAAAAGGTGGTCTAGACTCTTGGAGCTCAAGAATTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF177

Protein Analysis

239

Amino Acids

26.8

Weight (kDa)

9.14

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YceD PF02620 116 - 235 2.3e-10 Large ribosomal RNA subunit accumulation protein YceD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AclWI GGATC 2 cut(s) 66, 365
AcsI RAATTY 1 cut(s) 706
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 1 cut(s) 250
AgsI TTSAA 4 cut(s) 120, 239, 473, 512
AjnI CCWGG 2 cut(s) 46, 532
AluBI AGCT 6 cut(s) 43, 113, 505, 545, 613, 700
AluI AGCT 6 cut(s) 43, 113, 505, 545, 613, 700
Alw21I GWGCWC 1 cut(s) 702
AlwI GGATC 2 cut(s) 66, 365
ApeKI GCWGC 1 cut(s) 505
ApoI RAATTY 1 cut(s) 706
AspLEI GCGC 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 364, 529
BanII GRGCYC 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 1 cut(s) 492
BccI CCATC 4 cut(s) 192, 269, 284, 344
BceAI ACGGC 1 cut(s) 593
BciT130I CCWGG 2 cut(s) 48, 534
BclI TGATCA 1 cut(s) 171
BfaI CTAG 2 cut(s) 330, 687
BfmI CTRYAG 1 cut(s) 406
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 1 cut(s) 506
BlsI GCNGC 1 cut(s) 507
Bme1390I CCNGG 2 cut(s) 48, 534
BmrFI CCNGG 2 cut(s) 48, 534
BpuEI CTTGAG 1 cut(s) 686
Bsa29I ATCGAT 1 cut(s) 486
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 564
BseBI CCWGG 2 cut(s) 48, 534
BseCI ATCGAT 1 cut(s) 486
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 385
BseXI GCAGC 1 cut(s) 492
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 659
BshVI ATCGAT 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 702
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 702
Bsp143I GATC 4 cut(s) 71, 171, 357, 519
BspCNI CTCAG 1 cut(s) 106
BspDI ATCGAT 1 cut(s) 486
BspMAI CTGCAG 1 cut(s) 410
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 2 cut(s) 66, 365
BsrI ACTGG 1 cut(s) 564
BssMI GATC 4 cut(s) 71, 171, 357, 519
Bst2UI CCWGG 2 cut(s) 48, 534
Bst4CI ACNGT 2 cut(s) 426, 637
BstC8I GCNNGC 3 cut(s) 161, 327, 582
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 295
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 4 cut(s) 74, 174, 360, 522
BstMBI GATC 4 cut(s) 71, 171, 357, 519
BstNI CCWGG 2 cut(s) 48, 534
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 46, 532
BstSFI CTRYAG 1 cut(s) 406
BstV1I GCAGC 1 cut(s) 492
BstX2I RGATCY 2 cut(s) 71, 357
BstYI RGATCY 2 cut(s) 71, 357
Bsu15I ATCGAT 1 cut(s) 486
BsuTUI ATCGAT 1 cut(s) 486
BtsCI GGATG 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 2 cut(s) 172, 557
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 161, 327, 582
CfoI GCGC 1 cut(s) 165
ClaI ATCGAT 1 cut(s) 486
Csp6I GTAC 1 cut(s) 563
CviAII CATG 2 cut(s) 203, 326
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 1 cut(s) 93
DpnI GATC 4 cut(s) 73, 173, 359, 521
DpnII GATC 4 cut(s) 71, 171, 357, 519
Ecl136II GAGCTC 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 702
Eco53kI GAGCTC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 700
EcoRII CCWGG 2 cut(s) 46, 532
EcoT38I GRGCYC 1 cut(s) 702
FaeI CATG 2 cut(s) 206, 329
FaiI YATR 9 cut(s) 53, 204, 327, 403, 436, 525, 593, 616, 645
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 1 cut(s) 217
FatI CATG 2 cut(s) 202, 325
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 506
FokI GGATG 1 cut(s) 302
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 1 cut(s) 506
FspBI CTAG 2 cut(s) 330, 687
GlaI GCGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 506
GsaI CCCAGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 165
Hin1II CATG 2 cut(s) 206, 329
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 5 cut(s) 105, 469, 554, 569, 690
HphI GGTGA 2 cut(s) 364, 529
Hpy166II GTNNAC 2 cut(s) 307, 319
Hpy188I TCNGA 6 cut(s) 96, 104, 110, 141, 176, 610
Hpy188III TCNNGA 3 cut(s) 239, 687, 703
Hpy8I GTNNAC 2 cut(s) 307, 319
HpyAV CCTTC 3 cut(s) 236, 245, 348
HpyCH4III ACNGT 2 cut(s) 426, 637
HpyCH4V TGCA 2 cut(s) 408, 640
HpyF3I CTNAG 1 cut(s) 93
Hsp92II CATG 2 cut(s) 206, 329
HspAI GCGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 4 cut(s) 71, 171, 357, 519
LmnI GCTCC 2 cut(s) 398, 697
Lsp1109I GCAGC 1 cut(s) 492
MaeI CTAG 2 cut(s) 330, 687
MaeIII GTNAC 2 cut(s) 10, 386
MalI GATC 4 cut(s) 73, 173, 359, 521
MboI GATC 4 cut(s) 71, 171, 357, 519
MboII GAAGA 3 cut(s) 108, 278, 281
MflI RGATCY 2 cut(s) 71, 357
MhlI GDGCHC 1 cut(s) 702
MluCI AATT 3 cut(s) 16, 208, 706
MlyI GAGTC 2 cut(s) 563, 684
MmeI TCCRAC 1 cut(s) 656
MnlI CCTC 4 cut(s) 85, 320, 406, 598
MseI TTAA 5 cut(s) 39, 144, 362, 429, 497
MslI CAYNNNNRTG 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 505
MspR9I CCNGG 2 cut(s) 48, 534
MvaI CCWGG 2 cut(s) 48, 534
NdeII GATC 4 cut(s) 71, 171, 357, 519
NlaIII CATG 2 cut(s) 206, 329
NspI RCATGY 1 cut(s) 329
PaeI GCATGC 1 cut(s) 329
PfeI GAWTC 3 cut(s) 105, 469, 554
PkrI GCNGC 1 cut(s) 507
PleI GAGTC 2 cut(s) 563, 684
PpsI GAGTC 2 cut(s) 563, 684
Psp124BI GAGCTC 1 cut(s) 702
Psp6I CCWGG 2 cut(s) 46, 532
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 46, 532
PstI CTGCAG 1 cut(s) 410
PsuI RGATCY 2 cut(s) 71, 357
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
RseI CAYNNNNRTG 1 cut(s) 324
SacI GAGCTC 1 cut(s) 702
SaqAI TTAA 5 cut(s) 39, 144, 362, 429, 497
SatI GCNGC 1 cut(s) 506
Sau3AI GATC 4 cut(s) 71, 171, 357, 519
SchI GAGTC 2 cut(s) 563, 684
ScrFI CCNGG 2 cut(s) 48, 534
SduI GDGCHC 1 cut(s) 702
SfcI CTRYAG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SphI GCATGC 1 cut(s) 329
Sse9I AATT 3 cut(s) 16, 208, 706
SspI AATATT 1 cut(s) 37
SspMI CTAG 2 cut(s) 330, 687
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 2 cut(s) 46, 532
TaaI ACNGT 2 cut(s) 426, 637
TaqI TCGA 3 cut(s) 264, 449, 486
TasI AATT 3 cut(s) 16, 208, 706
TfiI GAWTC 3 cut(s) 105, 469, 554
Tru1I TTAA 5 cut(s) 39, 144, 362, 429, 497
Tru9I TTAA 5 cut(s) 39, 144, 362, 429, 497
TscAI CASTG 2 cut(s) 172, 564
TseI GCWGC 1 cut(s) 505
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 2 cut(s) 172, 564
XapI RAATTY 1 cut(s) 706
XbaI TCTAGA 1 cut(s) 686
XceI RCATGY 1 cut(s) 329
XspI CTAG 2 cut(s) 330, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.