Rh4BG094400

Uncharacterized ACR, COG1399

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013051
Physical Location & Seq
Reverse (-)
16098512 .. 16101111
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG094400.1

Sequence Viewer

Length: 696 bp
ATGGTTTTCAGAGAAATGGCAGAAGTTGCTAATTTGGTATCAGCAAGAAATATTAAGCTTCCTGGTATAAACCCAGCAAACCCCAGATCCTCCAAGTTGGTTTATTCTCAGAGTTTCAGAATCAGAGCTTCTTCAAAAAGAAACGACTTCTCTCTGATTAACAAAAAGAACAGCAGGCGCAGTGTGATCAGAATATCAACAGCAGATGGGAGATGGCATGGAAATTGGGGTTGTGATTACCTTCTGTCCCTTCAAGACCTGCGTTTGGGAGATTTGGTCGAAGAAGATGATGGAAATAAAAAGGATGCACAAGTTTCAGTCAACCTCTCCGTCCACAAGCATGCTAGTTTTGGCTTCTCTGTTGATGGAAGGATCTTAACATCATTCACCAGAAAATGTAGCAACTGCTCCTCTCCATACTGCAGAAAGATTGACACACAGTTTAATGTATGGGTTCTGTCATCAAGCAGAGATGACAACACAATTCAACTGCCAGAAATCGGTGGCGACGATCCATCATTAACTGAACTTGATTCACTGGTACAAGACTCAATACGGCTCGCCACCTCCATAAAAGATACTTGTTCAGAGCTATGTGAAAAATCTTATCCAACTGTGCAGTATATTGGTGGAGAGAGCAGGGCTTCTATTGATAAAAGGTGGTCTAGACTCTTGGAGCTCAAGAATTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF177

Protein Analysis

231

Amino Acids

25.98

Weight (kDa)

8.89

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YceD PF02620 121 - 228 4.1e-08 Large ribosomal RNA subunit accumulation protein YceD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 267
AclWI GGATC 3 cut(s) 81, 380, 506
AcsI RAATTY 1 cut(s) 685
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 2 cut(s) 265, 500
AgsI TTSAA 3 cut(s) 135, 254, 488
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 4 cut(s) 58, 128, 592, 679
AluI AGCT 4 cut(s) 58, 128, 592, 679
Alw21I GWGCWC 1 cut(s) 681
AlwI GGATC 3 cut(s) 81, 380, 506
ApoI RAATTY 1 cut(s) 685
AspLEI GCGC 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 379
BanII GRGCYC 1 cut(s) 681
Bbv12I GWGCWC 1 cut(s) 681
BccI CCATC 5 cut(s) 200, 207, 284, 359, 523
BceAI ACGGC 1 cut(s) 572
BciT130I CCWGG 1 cut(s) 63
BclI TGATCA 1 cut(s) 186
BfaI CTAG 2 cut(s) 345, 666
BfmI CTRYAG 1 cut(s) 421
BfuAI ACCTGC 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 665
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 500
Bse1I ACTGG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 2 cut(s) 265, 500
BseMII CTCAG 1 cut(s) 122
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 400
BseYI CCCAGC 1 cut(s) 73
BsgI GTGCAG 1 cut(s) 638
BsiHKAI GWGCWC 1 cut(s) 681
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 265, 500
BsmFI GGGAC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 681
Bsp143I GATC 4 cut(s) 86, 186, 372, 511
BspCNI CTCAG 1 cut(s) 121
BspMAI CTGCAG 1 cut(s) 425
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 3 cut(s) 81, 380, 506
BsrI ACTGG 1 cut(s) 543
BssMI GATC 4 cut(s) 86, 186, 372, 511
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 441, 616
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 176, 342, 561
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 4 cut(s) 89, 189, 375, 514
BstMBI GATC 4 cut(s) 86, 186, 372, 511
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 421
BstX2I RGATCY 2 cut(s) 86, 372
BstYI RGATCY 2 cut(s) 86, 372
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 187, 536
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 3 cut(s) 176, 342, 561
CfoI GCGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 542
CviAII CATG 2 cut(s) 218, 341
CviJI RGCY 7 cut(s) 58, 128, 354, 559, 592, 644, 679
CviKI_1 RGCY 7 cut(s) 58, 128, 354, 559, 592, 644, 679
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 1 cut(s) 108
DpnI GATC 4 cut(s) 88, 188, 374, 513
DpnII GATC 4 cut(s) 86, 186, 372, 511
Ecl136II GAGCTC 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 681
Eco53kI GAGCTC 1 cut(s) 679
EcoICRI GAGCTC 1 cut(s) 679
EcoRII CCWGG 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 681
FaeI CATG 2 cut(s) 221, 344
FaiI YATR 8 cut(s) 68, 219, 342, 418, 451, 572, 595, 624
FaqI GGGAC 1 cut(s) 232
FatI CATG 2 cut(s) 217, 340
FbaI TGATCA 1 cut(s) 186
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 681
FspBI CTAG 2 cut(s) 345, 666
GlaI GCGC 1 cut(s) 179
GsaI CCCAGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 180
Hin1II CATG 2 cut(s) 221, 344
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 120, 533, 548, 669
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 2 cut(s) 322, 334
Hpy188I TCNGA 7 cut(s) 11, 111, 119, 125, 156, 191, 589
Hpy188III TCNNGA 3 cut(s) 254, 666, 682
Hpy8I GTNNAC 2 cut(s) 322, 334
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 3 cut(s) 251, 260, 363
HpyCH4III ACNGT 2 cut(s) 441, 616
HpyCH4V TGCA 3 cut(s) 308, 423, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 2 cut(s) 221, 344
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 4 cut(s) 86, 186, 372, 511
LmnI GCTCC 2 cut(s) 413, 676
LweI GCATC 1 cut(s) 295
MaeI CTAG 2 cut(s) 345, 666
MalI GATC 4 cut(s) 88, 188, 374, 513
MboI GATC 4 cut(s) 86, 186, 372, 511
MboII GAAGA 3 cut(s) 123, 293, 296
MflI RGATCY 2 cut(s) 86, 372
MhlI GDGCHC 1 cut(s) 681
MluCI AATT 4 cut(s) 31, 223, 483, 685
MlyI GAGTC 2 cut(s) 542, 663
MmeI TCCRAC 1 cut(s) 635
MnlI CCTC 4 cut(s) 100, 335, 421, 577
MseI TTAA 5 cut(s) 54, 159, 377, 444, 521
MslI CAYNNNNRTG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 4 cut(s) 86, 186, 372, 511
NlaIII CATG 2 cut(s) 221, 344
NspI RCATGY 1 cut(s) 344
PaeI GCATGC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 2 cut(s) 120, 533
PleI GAGTC 2 cut(s) 542, 663
PpsI GAGTC 2 cut(s) 542, 663
Psp124BI GAGCTC 1 cut(s) 681
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 73
PspGI CCWGG 1 cut(s) 61
PstI CTGCAG 1 cut(s) 425
PsuI RGATCY 2 cut(s) 86, 372
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 1 cut(s) 339
SacI GAGCTC 1 cut(s) 681
SaqAI TTAA 5 cut(s) 54, 159, 377, 444, 521
Sau3AI GATC 4 cut(s) 86, 186, 372, 511
SchI GAGTC 2 cut(s) 542, 663
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 681
SetI ASST 9 cut(s) 60, 130, 243, 261, 327, 569, 594, 662, 681
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
SphI GCATGC 1 cut(s) 344
Sse9I AATT 4 cut(s) 31, 223, 483, 685
SspI AATATT 1 cut(s) 52
SspMI CTAG 2 cut(s) 345, 666
SstI GAGCTC 1 cut(s) 681
StyD4I CCNGG 1 cut(s) 61
TaaI ACNGT 2 cut(s) 441, 616
TaqI TCGA 1 cut(s) 279
TasI AATT 4 cut(s) 31, 223, 483, 685
TfiI GAWTC 2 cut(s) 120, 533
Tru1I TTAA 5 cut(s) 54, 159, 377, 444, 521
Tru9I TTAA 5 cut(s) 54, 159, 377, 444, 521
TscAI CASTG 2 cut(s) 187, 543
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 2 cut(s) 187, 543
XapI RAATTY 1 cut(s) 685
XbaI TCTAGA 1 cut(s) 665
XceI RCATGY 1 cut(s) 344
XspI CTAG 2 cut(s) 345, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.