Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012238

Physical Location & Seq

Genomic Coordinates Reverse (-)

31439181 .. 31440230

Gene Structure 1050 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1259000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 759 bp

ATGAAGGTTGAGAAGGAGAGTAGATCTGGATCATATGCACTTCTGCTAGCAGCAAGGGACTCAGAGTATGTGAAGAAAGTGTACGGTGGGTATTTCAATGTTTTTGTTGCAGCGTTTGGGGAAGAAGGAGAGAGGTGGGACTTGTACAGGGTTGTAGATGGAGAGTTTCCTGACATGGATGACCTTCAAAGCTATGATGGCTTCATCATCAGTGGCAGCCCTTATGATGCTTATGGCAATGACTACTGGATCATCAAACTATGCTTTCTCTTGCAAAGTTTGGATGCCATGGAGAAGAAAGTCCTCGGAATTTGCTTTGGTCATCAGGTGTTGTGCAGAGCACTAGGAGGAAAGGTTGGGAAAGCTTATACTGGGTGGGATATTGGGTTGAGGAAAGTGAAGCTAGTGAAGGAATTGGCTCCATTCAGCTTCGTTAATGACTTGGATGACGTTCCGCCTGCCCTTTCCATTATTGAGTGCCATCAAGATGAGGTTTGGGAAGTTCCTTTGGGTGCAGAGGTGATCGGGTACTCGGACAAAACAGGTGTGGAGATGTTCACCATTGGAGGCCACGTTCTGGGCATTCAAGGTCATCCTGAATACAGTAAGGACATTCTTTCAAATCTCATCGATCGCCTTTTCAACAGCGACTGCATAGAGAAAGGTTTTGCAGAAAACGCAAGGTCTGCACTGCAATTAGCTGAACCAGATAGGAAGTGCTGGGAGAAGTTCTGCAAGACATTTCTCAAGGGAAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006508 GO:0006725 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0008242 GO:0009058 GO:0009403 GO:0009404 GO:0009700 GO:0009987 GO:0010120 GO:0016143 GO:0016787 GO:0018130 GO:0019438 GO:0019538 GO:0019748 GO:0019757 GO:0019760 GO:0034641 GO:0034722 GO:0042430 GO:0042435 GO:0043170 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0052314 GO:0052315 GO:0052317 GO:0070011 GO:0071704 GO:0140096 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GATase

Protein Analysis

253

Amino Acids

28.29

Weight (kDa)

4.99

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	58 - 203	2.8e-14	Glutamine amidotransferase class-I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23970 AT4G30550
fragaria_vesca	FvH4_7g12720 FvH4_7g12720
malus_domestica	MD13G1275500.v1.1 MD16G1259000.v1.1
prunus_persica	Prupe.1G101400_v2.0.a1
pyrus_communis	pycom16g22650
rosa_chinensis	RchiOBHm_Chr4g0405351
rosa_laevigata	RLG00000008849
rosa_multiflora	Rmu_sc0000161.1_g000077
rosa_roxburghii	Rroxscaffold_5G00349780
rosa_rugosa	Rorug04G0057600
rosa_samantha	Rh4AG131100 Rh4BG125700 Rh4CG137800 Rh4CG138000 Rh4DG125100
rosa_wichuraiana	Rw4G010620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	577
AciI	CCGC	1 cut(s)	455
AclWI	GGATC	2 cut(s)	37, 257
AcsI	RAATTY	1 cut(s)	309
AfaI	GTAC	3 cut(s)	83, 146, 530
AfiI	CCNNNNNNNGG	1 cut(s)	577
AgsI	TTSAA	5 cut(s)	97, 188, 587, 621, 643
AloI	GAACNNNNNNTCC	2 cut(s)	558, 590
AluBI	AGCT	5 cut(s)	192, 365, 403, 429, 701
AluI	AGCT	5 cut(s)	192, 365, 403, 429, 701
Alw21I	GWGCWC	1 cut(s)	343
AlwI	GGATC	2 cut(s)	37, 257
AlwNI	CAGNNNCTG	1 cut(s)	651
AoxI	GGCC	1 cut(s)	568
ApeKI	GCWGC	3 cut(s)	50, 110, 216
ApoI	RAATTY	1 cut(s)	309
AsuHPI	GGTGA	2 cut(s)	532, 550
AsuNHI	GCTAGC	1 cut(s)	46
BarI	GAAGNNNNNNTAC	2 cut(s)	65, 97
Bbv12I	GWGCWC	1 cut(s)	343
BbvI	GCAGC	3 cut(s)	62, 122, 228
BccI	CCATC	3 cut(s)	152, 191, 489
BfaI	CTAG	3 cut(s)	47, 344, 404
BglII	AGATCT	1 cut(s)	23
BisI	GCNGC	3 cut(s)	51, 111, 217
BlsI	GCNGC	3 cut(s)	52, 112, 218
BmiI	GGNNCC	1 cut(s)	420
BmrI	ACTGGG	1 cut(s)	381
BmsI	GCATC	2 cut(s)	217, 274
BmtI	GCTAGC	1 cut(s)	50
BmuI	ACTGGG	1 cut(s)	381
BpuEI	CTTGAG	1 cut(s)	731
Bsa29I	ATCGAT	1 cut(s)	630
BsaBI	GATNNNNATC	1 cut(s)	28
BsaJI	CCNNGG	2 cut(s)	288, 304
BsaXI	ACNNNNNCTCC	4 cut(s)	339, 369, 558, 588
Bsc4I	CCNNNNNNNGG	1 cut(s)	577
Bse1I	ACTGG	2 cut(s)	251, 376
Bse3DI	GCAATG	1 cut(s)	244
Bse8I	GATNNNNATC	1 cut(s)	28
BseCI	ATCGAT	1 cut(s)	630
BseDI	CCNNGG	2 cut(s)	288, 304
BseGI	GGATG	4 cut(s)	184, 289, 451, 592
BseJI	GATNNNNATC	1 cut(s)	28
BseLI	CCNNNNNNNGG	1 cut(s)	577
BseMI	GCAATG	1 cut(s)	244
BseMII	CTCAG	1 cut(s)	75
BseNI	ACTGG	2 cut(s)	251, 376
BseXI	GCAGC	3 cut(s)	62, 122, 228
BseYI	CCCAGC	1 cut(s)	720
BsgI	GTGCAG	3 cut(s)	355, 534, 672
Bsh1285I	CGRYCG	1 cut(s)	634
BshFI	GGCC	1 cut(s)	570
BshVI	ATCGAT	1 cut(s)	630
BsiEI	CGRYCG	1 cut(s)	634
BsiHKAI	GWGCWC	1 cut(s)	343
BslFI	GGGAC	2 cut(s)	71, 152
BslI	CCNNNNNNNGG	1 cut(s)	577
BsmFI	GGGAC	2 cut(s)	71, 152
BsmI	GAATGC	1 cut(s)	582
BsnI	GGCC	1 cut(s)	570
Bsp1286I	GDGCHC	1 cut(s)	343
Bsp1407I	TGTACA	1 cut(s)	144
Bsp143I	GATC	5 cut(s)	23, 29, 249, 522, 631
Bsp19I	CCATGG	1 cut(s)	288
BspACI	CCGC	1 cut(s)	455
BspANI	GGCC	1 cut(s)	570
BspCNI	CTCAG	1 cut(s)	74
BspDI	ATCGAT	1 cut(s)	630
BspLI	GGNNCC	1 cut(s)	420
BspOI	GCTAGC	1 cut(s)	50
BspPI	GGATC	2 cut(s)	37, 257
BsrDI	GCAATG	1 cut(s)	244
BsrGI	TGTACA	1 cut(s)	144
BsrI	ACTGG	2 cut(s)	251, 376
BssECI	CCNNGG	2 cut(s)	288, 304
BssMI	GATC	5 cut(s)	23, 29, 249, 522, 631
BssT1I	CCWWGG	1 cut(s)	288
Bst4CI	ACNGT	2 cut(s)	86, 605
BstAPI	GCANNNNNTGC	1 cut(s)	686
BstAUI	TGTACA	1 cut(s)	144
BstC8I	GCNNGC	2 cut(s)	48, 459
BstDEI	CTNAG	1 cut(s)	61
BstDSI	CCRYGG	1 cut(s)	288
BstF5I	GGATG	4 cut(s)	184, 289, 451, 592
BstKTI	GATC	5 cut(s)	26, 32, 252, 525, 634
BstMBI	GATC	5 cut(s)	23, 29, 249, 522, 631
BstMCI	CGRYCG	1 cut(s)	634
BstMWI	GCNNNNNNNGC	3 cut(s)	198, 677, 686
BstV1I	GCAGC	3 cut(s)	62, 122, 228
BstX2I	RGATCY	1 cut(s)	23
BstYI	RGATCY	1 cut(s)	23
Bsu15I	ATCGAT	1 cut(s)	630
BsuRI	GGCC	1 cut(s)	570
BsuTUI	ATCGAT	1 cut(s)	630
BtgI	CCRYGG	1 cut(s)	288
BtsCI	GGATG	4 cut(s)	184, 289, 451, 592
BtsI	GCAGTG	1 cut(s)	689
BtsIMutI	CAGTG	2 cut(s)	217, 689
Cac8I	GCNNGC	2 cut(s)	48, 459
CaiI	CAGNNNCTG	1 cut(s)	651
ClaI	ATCGAT	1 cut(s)	630
Csp6I	GTAC	3 cut(s)	82, 145, 529
CviAII	CATG	2 cut(s)	175, 289
CviJI	RGCY	9 cut(s)	192, 201, 219, 365, 403, 419, 429, 570, 701
CviKI_1	RGCY	9 cut(s)	192, 201, 219, 365, 403, 419, 429, 570, 701
CviQI	GTAC	3 cut(s)	82, 145, 529
DdeI	CTNAG	1 cut(s)	61
DpnI	GATC	5 cut(s)	25, 31, 251, 524, 633
DpnII	GATC	5 cut(s)	23, 29, 249, 522, 631
EciI	GGCGGA	1 cut(s)	444
Eco130I	CCWWGG	1 cut(s)	288
EcoT14I	CCWWGG	1 cut(s)	288
ErhI	CCWWGG	1 cut(s)	288
FaeI	CATG	2 cut(s)	178, 292
FaqI	GGGAC	2 cut(s)	71, 152
FatI	CATG	2 cut(s)	174, 288
FauNDI	CATATG	1 cut(s)	34
Fnu4HI	GCNGC	3 cut(s)	51, 111, 217
FokI	GGATG	4 cut(s)	191, 296, 458, 579
Fsp4HI	GCNGC	3 cut(s)	51, 111, 217
FspBI	CTAG	3 cut(s)	47, 344, 404
GluI	GCNGC	3 cut(s)	51, 111, 217
GsaI	CCCAGC	1 cut(s)	724
HaeIII	GGCC	1 cut(s)	570
Hin1II	CATG	2 cut(s)	178, 292
HindIII	AAGCTT	1 cut(s)	363
HinfI	GANTC	1 cut(s)	59
HphI	GGTGA	2 cut(s)	532, 550
Hpy166II	GTNNAC	2 cut(s)	82, 558
Hpy188I	TCNGA	3 cut(s)	64, 308, 535
Hpy188III	TCNNGA	4 cut(s)	27, 170, 485, 596
Hpy8I	GTNNAC	2 cut(s)	82, 558
HpyAV	CCTTC	4 cut(s)	7, 119, 194, 403
HpyCH4III	ACNGT	2 cut(s)	86, 605
HpyCH4IV	ACGT	2 cut(s)	450, 573
HpyF10VI	GCNNNNNNNGC	3 cut(s)	198, 677, 686
HpyF3I	CTNAG	1 cut(s)	61
HpySE526I	ACGT	2 cut(s)	450, 573
Hsp92II	CATG	2 cut(s)	178, 292
Kzo9I	GATC	5 cut(s)	23, 29, 249, 522, 631
LmnI	GCTCC	1 cut(s)	424
Lsp1109I	GCAGC	3 cut(s)	62, 122, 228
LweI	GCATC	2 cut(s)	217, 274
MaeI	CTAG	3 cut(s)	47, 344, 404
MaeII	ACGT	2 cut(s)	450, 573
MalI	GATC	5 cut(s)	25, 31, 251, 524, 633
MboI	GATC	5 cut(s)	23, 29, 249, 522, 631
MboII	GAAGA	3 cut(s)	85, 134, 307
MflI	RGATCY	1 cut(s)	23
MhlI	GDGCHC	1 cut(s)	343
MluCI	AATT	3 cut(s)	309, 413, 695
MlyI	GAGTC	1 cut(s)	53
MnlI	CCTC	7 cut(s)	126, 314, 341, 384, 484, 511, 560
MseI	TTAA	1 cut(s)	435
MslI	CAYNNNNRTG	1 cut(s)	486
Mva1269I	GAATGC	1 cut(s)	582
MwoI	GCNNNNNNNGC	3 cut(s)	198, 677, 686
NcoI	CCATGG	1 cut(s)	288
NdeI	CATATG	1 cut(s)	34
NdeII	GATC	5 cut(s)	23, 29, 249, 522, 631
NheI	GCTAGC	1 cut(s)	46
NlaIII	CATG	2 cut(s)	178, 292
NlaIV	GGNNCC	1 cut(s)	420
PctI	GAATGC	1 cut(s)	582
PflMI	CCANNNNNTGG	1 cut(s)	577
PkrI	GCNGC	3 cut(s)	52, 112, 218
Ple19I	CGATCG	1 cut(s)	634
PleI	GAGTC	1 cut(s)	53
PpsI	GAGTC	1 cut(s)	53
PspFI	CCCAGC	1 cut(s)	720
PspN4I	GGNNCC	1 cut(s)	420
PstNI	CAGNNNCTG	1 cut(s)	651
PsuI	RGATCY	1 cut(s)	23
PvuI	CGATCG	1 cut(s)	634
RsaI	GTAC	3 cut(s)	83, 146, 530
RsaNI	GTAC	3 cut(s)	82, 145, 529
RseI	CAYNNNNRTG	1 cut(s)	486
SaqAI	TTAA	1 cut(s)	435
SatI	GCNGC	3 cut(s)	51, 111, 217
Sau3AI	GATC	5 cut(s)	23, 29, 249, 522, 631
SchI	GAGTC	1 cut(s)	53
SduI	GDGCHC	1 cut(s)	343
SfaNI	GCATC	2 cut(s)	217, 274
SmiMI	CAYNNNNRTG	1 cut(s)	486
SmlI	CTYRAG	1 cut(s)	746
SmoI	CTYRAG	1 cut(s)	746
Sse9I	AATT	3 cut(s)	309, 413, 695
SsiI	CCGC	1 cut(s)	455
SspMI	CTAG	3 cut(s)	47, 344, 404
StyI	CCWWGG	1 cut(s)	288
TaaI	ACNGT	2 cut(s)	86, 605
TaiI	ACGT	2 cut(s)	453, 576
TaqI	TCGA	1 cut(s)	630
TasI	AATT	3 cut(s)	309, 413, 695
TatI	WGTACW	1 cut(s)	144
Tru1I	TTAA	1 cut(s)	435
Tru9I	TTAA	1 cut(s)	435
TscAI	CASTG	2 cut(s)	217, 696
TseI	GCWGC	3 cut(s)	50, 110, 216
TspDTI	ATGAA	2 cut(s)	17, 193
TspRI	CASTG	2 cut(s)	217, 696
Van91I	CCANNNNNTGG	1 cut(s)	577
XapI	RAATTY	1 cut(s)	309
XspI	CTAG	3 cut(s)	47, 344, 404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.