Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014140

Physical Location & Seq

Genomic Coordinates Forward (+)

31545199 .. 31549493

Gene Structure 4295 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1259200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGCTTCCTCTCAAGCTCTTGATCTTCTTTCTCTCTTCTTGTTCCCTCTGCCTCTCCTATGAGCCTCGCAACCATGAAGTGGAGGCTTTGATAAGCTTAAGGGTAGGTTTGAATGATCCGCATGGGGTGTTAAACAACTGGGATGAGGACTCAGTGGACCCTTGTAGCTGGGCTATGATCACCTGCTCCCCTGATAATCTCGTCATTGGCCTGGGAGCTCCAAGCCAGTCTCTGTCTGGAACTCTGTCCGGGGCTTTTGCAAATCTCACTAATCTTCGACAAGTGTTGCTGCAGAACAACAATATATGTGGGAAACTGCCCTCGGAGCTGGGTACGCTGCCCAAGCTTCAAACCTTGGATCTCTCCAACAACCGATTTTCTGGTCTTGTCCCCGACTCTCTGGCTCACTTGAACACTCTCCAATACCTGAGGCTGAACAACAATAGCTTGTCTGGGCCTTTTCCCGTGTCTTTAGCCAAAATCCCCGACCTTGCTTTCTTGGACTTGTCTTATAACAATCTCAGTGGTCCAATACCCAAATTCCCAGCCAGAACTTTTAATGTTGTAGGGAATCCGCTAATTTGCGCAAGCAGCTCCACTGAAGGATGCTCTGGATCAGCTACTCCCGTCCCTCTTTCATTATCTCTCAAAACATCACCTGGAAAACATAACTCCAAGACTGTAGCAATTGCACTCGGGCTCAGCCTCAGTTGTGTGCTTGTCATAGTCCTATTGCTTGGAATACTCTGGCACAGGAAGAAGCAAAAAACCCAATCCATTTTGAACATCAGTGACATTCAAGAAGAGGGGATTGTGAGCTTGGGCAACCTCAGAAGCTTTACTTTCAAACAACTTCAACTTGCAACAGACAATTTTAGCTCCAAACACATACTTGGGGCTGGAGGTTTTGGCAATGTCTACAAGGGAAAGCTACCAGATGGGACTATGGTGGCAGTGAAACGTCTCAAGGATGTCACAGGAACAGCTGGGGAATCACAATTTCGGACTGAATTGGAGATGATAAGCCTAGCAGTGCATCGCAATTTGCTTCGGCTGATTGGATTTTGTGCTACCTTTAGCGAGAGGCTTCTGGTGTACCCTTACATGTCTAATGGCAGTGTAGCCGCCAGGCTCAGAGGAAAACCAGCACTAGATTGGAACACAAGGAAGAGGATTGCAATTGGAGCTGCAAGAGGTCTTCTGTATCTACACGAGCAATGCGATCCAAAGATTATACACAGGGATGTAAAGGCTGCAAATGTGCTCCTGGATGACTACTGTGAGGCTATTGTTGGCGATTTTGGTCTTGCGAAGCTCCTTGACCATGCTGACTCTCATGTCACCACTGCTGTCCGTGGCACTGTTGGGCATATTGCACCTGAATACCTTTCCACTGGTCAATCATCTGAAAAAACTGATGTTTTTGGATTTGGAATTCTCTTGATAGAGCTCATAACTGGAATGAGAGCTCTTGAGTTTGGGAAAACAGTTAATCAGAAAGGAGCCATTCTTGAGTGGGTGAAGAAAATACAGCAAGAAAAGAAGGTGGAAGTTTTGGTAGACAGAGAGCTGGGAAACAATTACGACCGGATTGAGGTGGGGGAAATGCTGCAAGTGGCTCTCCTGTGCACTCAGTACCTACCAGCCCACCGCCCCAAGATGTCGGAAGTGGTGAGGATGCTCGAAGGCGATGGGCTAGCGGAGAAGTGGGCAGCATCACATAATCAAAGTAATTCCAGCATGGATCGCTTTCAGAGTCACAACAGTAACAAAAGCAGCAGTCACACTGATGGCATTCATTCAAAACACGATGGAAATGAACGCGATCGCGGAAGCATGTTCAGCGCTTGGATAGACGAGGATGAGGATGAGAATTCTTTGGATTCCTATGCCATGGAACTCTCTGGTCCAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004713 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009987 GO:0010150 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019199 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0090693 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

639

Amino Acids

69.72

Weight (kDa)

6.66

Isoelectric Point (pI)

38.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	24 - 63	6.7e-13	Leucine rich repeat N-terminal domain
LRR_14	PF23598	86 - 167	7.2e-08	Leucine-rich repeat region
LRR_8	PF13855	114 - 174	4.6e-11	Leucine rich repeat
Pkinase	PF00069	293 - 558	4.5e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	295 - 561	5.6e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23950 AT4G30520
fragaria_vesca	FvH4_4g08870 FvH4_4g08870
malus_domestica	MD16G1259200.v1.1
prunus_persica	Prupe.1G100800_v2.0.a1
pyrus_communis	pycom16g22670
rosa_chinensis	RchiOBHm_Chr4g0405301
rosa_laevigata	RLG00000008859
rosa_multiflora	Rmu_sc0000161.1_g000017
rosa_roxburghii	Rroxscaffold_5G00349670
rosa_rugosa	Rorug04G0057100 Rorug04G0057200
rosa_samantha	Rh4AG130600 Rh4CG137500 Rh4DG124000
rosa_wichuraiana	Rw4G010570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	513
AarI	CACCTGC	1 cut(s)	191
AasI	GACNNNNNNGTC	1 cut(s)	1337
Acc16I	TGCGCA	1 cut(s)	586
Acc36I	ACCTGC	1 cut(s)	191
AccB7I	CCANNNNNTGG	1 cut(s)	79
AccI	GTMKAC	2 cut(s)	918, 1560
AccII	CGCG	2 cut(s)	1824, 1830
AciI	CCGC	6 cut(s)	119, 575, 1125, 1651, 1700, 1830
AclWI	GGATC	5 cut(s)	110, 366, 622, 1217, 1752
AcsI	RAATTY	3 cut(s)	539, 1434, 1873
AcuI	CTGAAG	1 cut(s)	621
AfaI	GTAC	3 cut(s)	334, 1097, 1637
AfeI	AGCGCT	1 cut(s)	1846
AfiI	CCNNNNNNNGG	2 cut(s)	79, 1594
AflII	CTTAAG	1 cut(s)	97
AflIII	ACRYGT	1 cut(s)	1104
AgsI	TTSAA	8 cut(s)	112, 350, 412, 784, 800, 847, 857, 1803
AjnI	CCWGG	4 cut(s)	210, 658, 1127, 1266
Alw21I	GWGCWC	5 cut(s)	220, 1266, 1452, 1471, 1631
Alw26I	GTCTC	2 cut(s)	234, 968
Alw44I	GTGCAC	1 cut(s)	1627
AlwI	GGATC	5 cut(s)	110, 366, 622, 1217, 1752
AlwNI	CAGNNNCTG	1 cut(s)	232
Ama87I	CYCGRG	1 cut(s)	695
Aor51HI	AGCGCT	1 cut(s)	1846
AoxI	GGCC	2 cut(s)	208, 455
ApaLI	GTGCAC	1 cut(s)	1627
ApeKI	GCWGC	8 cut(s)	289, 337, 591, 1187, 1253, 1609, 1712, 1776
ApoI	RAATTY	3 cut(s)	539, 1434, 1873
AsiSI	GCGATCGC	1 cut(s)	1828
AspLEI	GCGC	2 cut(s)	587, 1847
AspS9I	GGNCC	4 cut(s)	157, 455, 527, 1907
AsuC2I	CCSGG	1 cut(s)	250
AsuHPI	GGTGA	5 cut(s)	172, 648, 1333, 1531, 1684
AsuNHI	GCTAGC	1 cut(s)	1696
AvaI	CYCGRG	1 cut(s)	695
AvaII	GGWCC	3 cut(s)	157, 527, 1907
AxyI	CCTNAGG	1 cut(s)	428
BaeGI	GKGCMC	1 cut(s)	1631
BanII	GRGCYC	4 cut(s)	220, 702, 1452, 1471
BauI	CACGAG	1 cut(s)	1211
BbsI	GAAGAC	1 cut(s)	1190
Bbv12I	GWGCWC	5 cut(s)	220, 1266, 1452, 1471, 1631
BbvI	GCAGC	8 cut(s)	276, 324, 603, 1174, 1240, 1596, 1724, 1788
BccI	CCATC	4 cut(s)	932, 1685, 1784, 1805
BciT130I	CCWGG	4 cut(s)	212, 660, 1129, 1268
BclI	TGATCA	1 cut(s)	177
BcnI	CCSGG	1 cut(s)	250
BcoDI	GTCTC	2 cut(s)	234, 968
BfaI	CTAG	3 cut(s)	1028, 1151, 1697
BfmI	CTRYAG	2 cut(s)	290, 681
BfoI	RGCGCY	1 cut(s)	1848
BfrI	CTTAAG	1 cut(s)	97
BfuAI	ACCTGC	1 cut(s)	191
BisI	GCNGC	9 cut(s)	290, 338, 592, 1125, 1188, 1254, 1610, 1713, 1777
BlpI	GCTNAGC	1 cut(s)	701
BlsI	GCNGC	9 cut(s)	291, 339, 593, 1126, 1189, 1255, 1611, 1714, 1778
Bme1390I	CCNGG	5 cut(s)	212, 250, 660, 1129, 1268
Bme18I	GGWCC	3 cut(s)	157, 527, 1907
BmeT110I	CYCGRG	1 cut(s)	695
BmgT120I	GGNCC	4 cut(s)	157, 455, 527, 1907
BmiI	GGNNCC	2 cut(s)	159, 1504
BmrFI	CCNGG	5 cut(s)	212, 250, 660, 1129, 1268
BmrI	ACTGGG	1 cut(s)	148
BmsI	GCATC	4 cut(s)	596, 1045, 1668, 1724
BmtI	GCTAGC	1 cut(s)	1700
BmuI	ACTGGG	1 cut(s)	148
BpiI	GAAGAC	1 cut(s)	1190
BpmI	CTGGAG	1 cut(s)	921
Bpu1102I	GCTNAGC	1 cut(s)	701
BpuEI	CTTGAG	3 cut(s)	950, 1493, 1532
BpuMI	CCSGG	1 cut(s)	250
BsaJI	CCNNGG	6 cut(s)	211, 249, 321, 354, 1354, 1893
BsaWI	WCCGGW	1 cut(s)	1587
Bsc4I	CCNNNNNNNGG	2 cut(s)	79, 1594
Bse1I	ACTGG	4 cut(s)	143, 226, 1399, 1462
Bse21I	CCTNAGG	1 cut(s)	428
Bse3DI	GCAATG	2 cut(s)	919, 1223
BseBI	CCWGG	4 cut(s)	212, 660, 1129, 1268
BseDI	CCNNGG	6 cut(s)	211, 249, 321, 354, 1354, 1893
BseGI	GGATG	8 cut(s)	148, 611, 976, 1249, 1276, 1683, 1867, 1873
BseLI	CCNNNNNNNGG	2 cut(s)	79, 1594
BseMI	GCAATG	2 cut(s)	919, 1223
BseMII	CTCAG	8 cut(s)	165, 419, 535, 715, 721, 844, 1147, 1646
BseNI	ACTGG	4 cut(s)	143, 226, 1399, 1462
BseSI	GKGCMC	1 cut(s)	1631
BseXI	GCAGC	8 cut(s)	276, 324, 603, 1174, 1240, 1596, 1724, 1788
BseYI	CCCAGC	5 cut(s)	168, 328, 544, 986, 1570
Bsh1236I	CGCG	2 cut(s)	1824, 1830
Bsh1285I	CGRYCG	2 cut(s)	1588, 1828
BshFI	GGCC	2 cut(s)	210, 457
BsiEI	CGRYCG	2 cut(s)	1588, 1828
BsiHKAI	GWGCWC	5 cut(s)	220, 1266, 1452, 1471, 1631
BsiHKCI	CYCGRG	1 cut(s)	695
BsiSI	CCGG	2 cut(s)	249, 1588
BslFI	GGGAC	3 cut(s)	374, 614, 955
BslI	CCNNNNNNNGG	2 cut(s)	79, 1594
BsmAI	GTCTC	2 cut(s)	234, 968
BsmBI	CGTCTC	1 cut(s)	968
BsmFI	GGGAC	3 cut(s)	374, 614, 955
BsmI	GAATGC	1 cut(s)	1794
BsnI	GGCC	2 cut(s)	210, 457
BsoBI	CYCGRG	1 cut(s)	695
Bsp1286I	GDGCHC	6 cut(s)	220, 702, 1266, 1452, 1471, 1631
Bsp143I	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
Bsp1720I	GCTNAGC	1 cut(s)	701
Bsp19I	CCATGG	1 cut(s)	1893
BspACI	CCGC	6 cut(s)	119, 575, 1125, 1651, 1700, 1830
BspANI	GGCC	2 cut(s)	210, 457
BspCNI	CTCAG	8 cut(s)	164, 420, 534, 714, 720, 843, 1146, 1645
BspFNI	CGCG	2 cut(s)	1824, 1830
BspLI	GGNNCC	2 cut(s)	159, 1504
BspMAI	CTGCAG	1 cut(s)	294
BspMI	ACCTGC	1 cut(s)	191
BspOI	GCTAGC	1 cut(s)	1700
BspPI	GGATC	5 cut(s)	110, 366, 622, 1217, 1752
BspTI	CTTAAG	1 cut(s)	97
BsrDI	GCAATG	2 cut(s)	919, 1223
BsrI	ACTGG	4 cut(s)	143, 226, 1399, 1462
BssECI	CCNNGG	6 cut(s)	211, 249, 321, 354, 1354, 1893
BssMI	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
BssSI	CACGAG	1 cut(s)	1211
BssT1I	CCWWGG	2 cut(s)	354, 1893
Bst2BI	CACGAG	1 cut(s)	1211
Bst2UI	CCWGG	4 cut(s)	212, 660, 1129, 1268
Bst4CI	ACNGT	5 cut(s)	682, 1280, 1363, 1489, 1766
Bst6I	CTCTTC	3 cut(s)	40, 798, 1163
BstAFI	CTTAAG	1 cut(s)	97
BstC8I	GCNNGC	2 cut(s)	589, 1698
BstDEI	CTNAG	8 cut(s)	151, 428, 521, 701, 707, 830, 1133, 1632
BstDSI	CCRYGG	2 cut(s)	1354, 1893
BstF5I	GGATG	8 cut(s)	148, 611, 976, 1249, 1276, 1683, 1867, 1873
BstFNI	CGCG	2 cut(s)	1824, 1830
BstH2I	RGCGCY	1 cut(s)	1848
BstHHI	GCGC	2 cut(s)	587, 1847
BstKTI	GATC	8 cut(s)	24, 118, 180, 361, 617, 1225, 1747, 1828
BstMAI	GTCTC	2 cut(s)	234, 968
BstMBI	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
BstMCI	CGRYCG	2 cut(s)	1588, 1828
BstMWI	GCNNNNNNNGC	7 cut(s)	325, 334, 343, 591, 1184, 1746, 1842
BstNI	CCWGG	4 cut(s)	212, 660, 1129, 1268
BstNSI	RCATGY	2 cut(s)	1108, 1840
BstSCI	CCNGG	5 cut(s)	210, 248, 658, 1127, 1266
BstSFI	CTRYAG	2 cut(s)	290, 681
BstSLI	GKGCMC	1 cut(s)	1631
BstUI	CGCG	2 cut(s)	1824, 1830
BstV1I	GCAGC	8 cut(s)	276, 324, 603, 1174, 1240, 1596, 1724, 1788
BstV2I	GAAGAC	1 cut(s)	1190
BstX2I	RGATCY	1 cut(s)	358
BstYI	RGATCY	1 cut(s)	358
Bsu36I	CCTNAGG	1 cut(s)	428
BsuRI	GGCC	2 cut(s)	210, 457
BtgI	CCRYGG	2 cut(s)	1354, 1893
BtgZI	GCGATG	2 cut(s)	1022, 1704
BtsCI	GGATG	8 cut(s)	148, 611, 976, 1249, 1276, 1683, 1867, 1873
BtsI	GCAGTG	4 cut(s)	960, 1038, 1123, 1344
BveI	ACCTGC	1 cut(s)	191
Cac8I	GCNNGC	2 cut(s)	589, 1698
CaiI	CAGNNNCTG	1 cut(s)	232
CfoI	GCGC	2 cut(s)	587, 1847
Cfr13I	GGNCC	4 cut(s)	157, 455, 527, 1907
Csp6I	GTAC	3 cut(s)	333, 1096, 1636
CspCI	CAANNNNNGTGG	4 cut(s)	289, 324, 505, 540
CviAII	CATG	8 cut(s)	74, 122, 1105, 1325, 1337, 1741, 1837, 1894
CviQI	GTAC	3 cut(s)	333, 1096, 1636
DdeI	CTNAG	8 cut(s)	151, 428, 521, 701, 707, 830, 1133, 1632
DpnI	GATC	8 cut(s)	23, 117, 179, 360, 616, 1224, 1746, 1827
DpnII	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
DrdI	GACNNNNNNGTC	1 cut(s)	1337
DseDI	GACNNNNNNGTC	1 cut(s)	1337
Eam1104I	CTCTTC	3 cut(s)	40, 798, 1163
EarI	CTCTTC	3 cut(s)	40, 798, 1163
Ecl136II	GAGCTC	3 cut(s)	218, 1450, 1469
Eco130I	CCWWGG	2 cut(s)	354, 1893
Eco24I	GRGCYC	4 cut(s)	220, 702, 1452, 1471
Eco47I	GGWCC	3 cut(s)	157, 527, 1907
Eco47III	AGCGCT	1 cut(s)	1846
Eco53kI	GAGCTC	3 cut(s)	218, 1450, 1469
Eco57I	CTGAAG	1 cut(s)	621
Eco81I	CCTNAGG	1 cut(s)	428
Eco88I	CYCGRG	1 cut(s)	695
EcoICRI	GAGCTC	3 cut(s)	218, 1450, 1469
EcoRI	GAATTC	2 cut(s)	1434, 1873
EcoRII	CCWGG	4 cut(s)	210, 658, 1127, 1266
EcoT14I	CCWWGG	2 cut(s)	354, 1893
EcoT38I	GRGCYC	4 cut(s)	220, 702, 1452, 1471
ErhI	CCWWGG	2 cut(s)	354, 1893
Esp3I	CGTCTC	1 cut(s)	968
FaeI	CATG	8 cut(s)	77, 125, 1108, 1328, 1340, 1744, 1840, 1897
FalI	AAGNNNNNCTT	2 cut(s)	826, 858
FaqI	GGGAC	3 cut(s)	374, 614, 955
FatI	CATG	8 cut(s)	73, 121, 1104, 1324, 1336, 1740, 1836, 1893
FbaI	TGATCA	1 cut(s)	177
FblI	GTMKAC	2 cut(s)	918, 1560
Fnu4HI	GCNGC	9 cut(s)	290, 338, 592, 1125, 1188, 1254, 1610, 1713, 1777
FokI	GGATG	8 cut(s)	155, 618, 983, 1256, 1283, 1690, 1874, 1880
FriOI	GRGCYC	4 cut(s)	220, 702, 1452, 1471
Fsp4HI	GCNGC	9 cut(s)	290, 338, 592, 1125, 1188, 1254, 1610, 1713, 1777
FspBI	CTAG	3 cut(s)	1028, 1151, 1697
FspI	TGCGCA	1 cut(s)	586
GlaI	GCGC	2 cut(s)	586, 1846
GluI	GCNGC	9 cut(s)	290, 338, 592, 1125, 1188, 1254, 1610, 1713, 1777
GsaI	CCCAGC	5 cut(s)	172, 332, 548, 990, 1574
GsuI	CTGGAG	1 cut(s)	921
HaeII	RGCGCY	1 cut(s)	1848
HaeIII	GGCC	2 cut(s)	210, 457
HapII	CCGG	2 cut(s)	249, 1588
HhaI	GCGC	2 cut(s)	587, 1847
Hin1II	CATG	8 cut(s)	77, 125, 1108, 1328, 1340, 1744, 1840, 1897
Hin6I	GCGC	2 cut(s)	585, 1845
HinP1I	GCGC	2 cut(s)	585, 1845
HindIII	AAGCTT	3 cut(s)	94, 344, 835
HinfI	GANTC	7 cut(s)	149, 395, 571, 992, 1331, 1756, 1883
HpaII	CCGG	2 cut(s)	249, 1588
HphI	GGTGA	5 cut(s)	172, 648, 1333, 1531, 1684
Hpy166II	GTNNAC	5 cut(s)	157, 919, 1096, 1561, 1629
Hpy188I	TCNGA	8 cut(s)	325, 833, 1005, 1136, 1408, 1497, 1666, 1755
Hpy188III	TCNNGA	7 cut(s)	19, 237, 612, 800, 1441, 1472, 1511
Hpy8I	GTNNAC	5 cut(s)	157, 919, 1096, 1561, 1629
HpyAV	CCTTC	3 cut(s)	596, 1537, 1679
HpyCH4III	ACNGT	5 cut(s)	682, 1280, 1363, 1489, 1766
HpyCH4IV	ACGT	1 cut(s)	961
HpyF10VI	GCNNNNNNNGC	7 cut(s)	325, 334, 343, 591, 1184, 1746, 1842
HpyF3I	CTNAG	8 cut(s)	151, 428, 521, 701, 707, 830, 1133, 1632
HpySE526I	ACGT	1 cut(s)	961
Hsp92II	CATG	8 cut(s)	77, 125, 1108, 1328, 1340, 1744, 1840, 1897
HspAI	GCGC	2 cut(s)	585, 1845
Ksp22I	TGATCA	1 cut(s)	177
Kzo9I	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
Lsp1109I	GCAGC	8 cut(s)	276, 324, 603, 1174, 1240, 1596, 1724, 1788
LweI	GCATC	4 cut(s)	596, 1045, 1668, 1724
MaeI	CTAG	3 cut(s)	1028, 1151, 1697
MaeII	ACGT	1 cut(s)	961
MaeIII	GTNAC	6 cut(s)	791, 973, 1339, 1757, 1766, 1781
MalI	GATC	8 cut(s)	23, 117, 179, 360, 616, 1224, 1746, 1827
MboI	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
MboII	GAAGA	8 cut(s)	16, 27, 266, 769, 815, 1180, 1190, 1534
MfeI	CAATTG	2 cut(s)	687, 1179
MflI	RGATCY	1 cut(s)	358
MhlI	GDGCHC	6 cut(s)	220, 702, 1266, 1452, 1471, 1631
MlyI	GAGTC	4 cut(s)	143, 389, 1325, 1765
MmeI	TCCRAC	2 cut(s)	390, 1644
MseI	TTAA	4 cut(s)	98, 131, 558, 1491
MslI	CAYNNNNRTG	2 cut(s)	1242, 1788
MspA1I	CMGCKG	1 cut(s)	986
MspCI	CTTAAG	1 cut(s)	97
MspI	CCGG	2 cut(s)	249, 1588
MspR9I	CCNGG	5 cut(s)	212, 250, 660, 1129, 1268
MunI	CAATTG	2 cut(s)	687, 1179
Mva1269I	GAATGC	1 cut(s)	1794
MvaI	CCWGG	4 cut(s)	212, 660, 1129, 1268
MvnI	CGCG	2 cut(s)	1824, 1830
MwoI	GCNNNNNNNGC	7 cut(s)	325, 334, 343, 591, 1184, 1746, 1842
NciI	CCSGG	1 cut(s)	250
NcoI	CCATGG	1 cut(s)	1893
NdeII	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
NheI	GCTAGC	1 cut(s)	1696
NlaIII	CATG	8 cut(s)	77, 125, 1108, 1328, 1340, 1744, 1840, 1897
NlaIV	GGNNCC	2 cut(s)	159, 1504
NmuCI	GTSAC	5 cut(s)	791, 973, 1339, 1757, 1781
NsbI	TGCGCA	1 cut(s)	586
NspI	RCATGY	2 cut(s)	1108, 1840
PaqCI	CACCTGC	1 cut(s)	191
PciI	ACATGT	1 cut(s)	1104
PcsI	WCGNNNNNNNCGW	1 cut(s)	1218
PctI	GAATGC	1 cut(s)	1794
PfeI	GAWTC	3 cut(s)	571, 992, 1883
PflMI	CCANNNNNTGG	1 cut(s)	79
PfoI	TCCNGGA	1 cut(s)	1266
PkrI	GCNGC	9 cut(s)	291, 339, 593, 1126, 1189, 1255, 1611, 1714, 1778
Ple19I	CGATCG	1 cut(s)	1828
PleI	GAGTC	4 cut(s)	143, 389, 1325, 1764
PpsI	GAGTC	4 cut(s)	143, 389, 1325, 1764
PscI	ACATGT	1 cut(s)	1104
PsiI	TTATAA	1 cut(s)	513
Psp124BI	GAGCTC	3 cut(s)	220, 1452, 1471
Psp6I	CCWGG	4 cut(s)	210, 658, 1127, 1266
PspFI	CCCAGC	5 cut(s)	168, 328, 544, 986, 1570
PspGI	CCWGG	4 cut(s)	210, 658, 1127, 1266
PspN4I	GGNNCC	2 cut(s)	159, 1504
PspPI	GGNCC	4 cut(s)	157, 455, 527, 1907
PstI	CTGCAG	1 cut(s)	294
PstNI	CAGNNNCTG	1 cut(s)	232
PsuI	RGATCY	1 cut(s)	358
PvuI	CGATCG	1 cut(s)	1828
PvuII	CAGCTG	1 cut(s)	986
RgaI	GCGATCGC	1 cut(s)	1828
RsaI	GTAC	3 cut(s)	334, 1097, 1637
RsaNI	GTAC	3 cut(s)	333, 1096, 1636
RseI	CAYNNNNRTG	2 cut(s)	1242, 1788
SacI	GAGCTC	3 cut(s)	220, 1452, 1471
SaqAI	TTAA	4 cut(s)	98, 131, 558, 1491
SatI	GCNGC	9 cut(s)	290, 338, 592, 1125, 1188, 1254, 1610, 1713, 1777
Sau3AI	GATC	8 cut(s)	21, 115, 177, 358, 614, 1222, 1744, 1825
Sau96I	GGNCC	4 cut(s)	157, 455, 527, 1907
SchI	GAGTC	4 cut(s)	143, 389, 1325, 1765
ScrFI	CCNGG	5 cut(s)	212, 250, 660, 1129, 1268
SduI	GDGCHC	6 cut(s)	220, 702, 1266, 1452, 1471, 1631
SfaAI	GCGATCGC	1 cut(s)	1828
SfaNI	GCATC	4 cut(s)	596, 1045, 1668, 1724
SfcI	CTRYAG	2 cut(s)	290, 681
SgfI	GCGATCGC	1 cut(s)	1828
SinI	GGWCC	3 cut(s)	157, 527, 1907
SmiMI	CAYNNNNRTG	2 cut(s)	1242, 1788
SmlI	CTYRAG	5 cut(s)	11, 97, 965, 1472, 1511
SmoI	CTYRAG	5 cut(s)	11, 97, 965, 1472, 1511
SsiI	CCGC	6 cut(s)	119, 575, 1125, 1651, 1700, 1830
SspMI	CTAG	3 cut(s)	1028, 1151, 1697
SstI	GAGCTC	3 cut(s)	220, 1452, 1471
StyD4I	CCNGG	5 cut(s)	210, 248, 658, 1127, 1266
StyI	CCWWGG	2 cut(s)	354, 1893
TaaI	ACNGT	5 cut(s)	682, 1280, 1363, 1489, 1766
TaiI	ACGT	1 cut(s)	964
TaqI	TCGA	2 cut(s)	277, 1683
TauI	GCSGC	1 cut(s)	1127
TfiI	GAWTC	3 cut(s)	571, 992, 1883
Tru1I	TTAA	4 cut(s)	98, 131, 558, 1491
Tru9I	TTAA	4 cut(s)	98, 131, 558, 1491
TseFI	GTSAC	5 cut(s)	791, 973, 1339, 1757, 1781
TseI	GCWGC	8 cut(s)	289, 337, 591, 1187, 1253, 1609, 1712, 1776
Tsp45I	GTSAC	5 cut(s)	791, 973, 1339, 1757, 1781
TspDTI	ATGAA	4 cut(s)	90, 627, 1787, 1833
TspGWI	ACGGA	1 cut(s)	1343
Van91I	CCANNNNNTGG	1 cut(s)	79
Vha464I	CTTAAG	1 cut(s)	97
VneI	GTGCAC	1 cut(s)	1627
VpaK11BI	GGWCC	3 cut(s)	157, 527, 1907
XapI	RAATTY	3 cut(s)	539, 1434, 1873
XceI	RCATGY	2 cut(s)	1108, 1840
XcmI	CCANNNNNNNNNTGG	2 cut(s)	233, 1152
XmiI	GTMKAC	2 cut(s)	918, 1560
XspI	CTAG	3 cut(s)	1028, 1151, 1697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.