MD16G1261500.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015286
Physical Location & Seq
Forward (+)
32429244 .. 32431941
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1261500.v1.1.491

Sequence Viewer

Length: 801 bp
ATGAGGATAAGCAACAACCTGGTGGGTCTCCTCAACTTCATAACGTTCCTGCTATCGATTCCGATCGTGGTGGCCGGAGTATGGCTGACCAAGCAAGGCAGTACCGAGTGCGAGAAGTTCCTAGACAAGCCCGTCATCATCCTCGGCGTATTCCTCATGCTGGTGTCGCTGGCCGGCCTAATTGGCGCCTGCTGCCGCGTCTCGTGGCTGCTGTGGGTCTACCTCCTCGTCATGTTCCTCCTCATCGTGGTCCTCTTCGCCGTCACCATCTTCGCCTTCGCCGTCACCAACAAAGGCGCCGGAAAAGTACTTTCCGGGAAAGGGTACAAGGAGTACAGGCTCGGGGACTACTCGCAGTGGCTGCAGAAGAGGGTTAACAGCACCAAGAACTGGAACAAGATCAAGAGCTGTCTCATTGACAGCAAGGTCTGCTCTACTTTCAAGGACAAGTATGCTAATGATACACTCCAGACCTTCTATTCTGAGAACCTGTCAGCGCTTCAGGCCGGTTGCTGTAAGCCATCAGATGATTGTGGATTTACTTACGAAAGCCCCATCTCCTGGACCAGCAACGTTACTACCGTTTCCTCCGACCCTGACTGCAGTGCGTGGCAGAATGATGAGAAGGTTCTGTGCTTCAACTGTCAGTCGTGCAAGGCTGGACTGCTGGACAACATAAAAAGTAATTGGAAGAAGACGGCGATTGTCAACATTGTCTTCCTCATCTTCCTGATTGTGGTGTATTCAGTTGGATGCTGCGCGTTTAGGAACAGCAGGAGGGACAATGAATATTGGAAATAA

Protein Analysis

267

Amino Acids

29.87

Weight (kDa)

8.87

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 254 8.1e-40 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23810
fragaria_vesca FvH4_4g10210
malus_domestica MD13G1273700.v1.1 MD16G1261500.v1.1
prunus_persica Prupe.1G098200_v2.0.a1
pyrus_communis pycom13g28840 pycom16g22990
rosa_chinensis RchiOBHm_Chr4g0405031
rosa_laevigata RLG00000008883
rosa_multiflora Rmu_ssc0000127.1_g000047
rosa_roxburghii Rroxscaffold_5G00349210
rosa_rugosa Rorug04G0052400
rosa_samantha Rh4AG127300 Rh4BG121200 Rh4CG135300
rosa_wichuraiana Rw4G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 131, 425
AccB1I GGYRCC 2 cut(s) 185, 296
AccB7I CCANNNNNTGG 2 cut(s) 390, 561
AccI GTMKAC 1 cut(s) 219
AccII CGCG 2 cut(s) 198, 761
AciI CCGC 1 cut(s) 196
AclI AACGTT 2 cut(s) 44, 573
AcoI YGGCCR 2 cut(s) 72, 171
AcuI CTGAAG 1 cut(s) 485
AcyI GRCGYC 2 cut(s) 186, 297
AfaI GTAC 4 cut(s) 103, 309, 326, 335
AfeI AGCGCT 1 cut(s) 498
AfiI CCNNNNNNNGG 7 cut(s) 81, 160, 247, 321, 390, 561, 736
AgsI TTSAA 2 cut(s) 442, 640
AjnI CCWGG 2 cut(s) 18, 560
AluBI AGCT 1 cut(s) 408
AluI AGCT 1 cut(s) 408
Alw26I GTCTC 3 cut(s) 32, 205, 416
AlwNI CAGNNNCTG 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 498
AoxI GGCC 4 cut(s) 72, 171, 175, 504
ApeKI GCWGC 4 cut(s) 192, 208, 361, 756
AspLEI GCGC 4 cut(s) 188, 299, 499, 761
AspS9I GGNCC 2 cut(s) 250, 564
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 2 cut(s) 256, 277
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 2 cut(s) 250, 564
BanI GGYRCC 2 cut(s) 185, 296
BauI CACGAG 1 cut(s) 202
BbsI GAAGAC 2 cut(s) 701, 709
BbvI GCAGC 4 cut(s) 179, 195, 348, 743
BccI CCATC 3 cut(s) 275, 529, 563
BceAI ACGGC 3 cut(s) 245, 266, 714
BciT130I CCWGG 2 cut(s) 20, 562
BcnI CCSGG 1 cut(s) 316
BcoDI GTCTC 3 cut(s) 32, 205, 416
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 2 cut(s) 362, 601
BfoI RGCGCY 3 cut(s) 189, 300, 500
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 5 cut(s) 193, 196, 209, 362, 757
BlsI GCNGC 5 cut(s) 194, 197, 210, 363, 758
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 3 cut(s) 20, 316, 562
Bme18I GGWCC 2 cut(s) 250, 564
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 250, 564
BmiI GGNNCC 2 cut(s) 187, 298
BmrFI CCNGG 3 cut(s) 20, 316, 562
BmsI GCATC 1 cut(s) 743
BpiI GAAGAC 2 cut(s) 701, 709
BpmI CTGGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 316
Bsa29I ATCGAT 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 186, 297
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 142
Bsc4I CCNNNNNNNGG 7 cut(s) 81, 160, 247, 321, 390, 561, 736
Bse118I RCCGGY 2 cut(s) 173, 506
Bse1I ACTGG 1 cut(s) 395
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 2 cut(s) 20, 562
BseCI ATCGAT 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 138, 758
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 7 cut(s) 81, 160, 247, 321, 390, 561, 736
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 3 cut(s) 20, 215, 230
BseXI GCAGC 4 cut(s) 179, 195, 348, 743
Bsh1236I CGCG 2 cut(s) 198, 761
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 74, 173, 177, 506
BshNI GGYRCC 2 cut(s) 185, 296
BshVI ATCGAT 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 5 cut(s) 75, 174, 300, 315, 507
BslFI GGGAC 2 cut(s) 359, 794
BslI CCNNNNNNNGG 7 cut(s) 81, 160, 247, 321, 390, 561, 736
BsmAI GTCTC 3 cut(s) 32, 205, 416
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 2 cut(s) 359, 794
BsnI GGCC 4 cut(s) 74, 173, 177, 506
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 341
Bsp143I GATC 2 cut(s) 63, 399
BspACI CCGC 1 cut(s) 196
BspANI GGCC 4 cut(s) 74, 173, 177, 506
BspCNI CTCAG 1 cut(s) 475
BspDI ATCGAT 1 cut(s) 56
BspFNI CGCG 2 cut(s) 198, 761
BspLI GGNNCC 2 cut(s) 187, 298
BspMAI CTGCAG 2 cut(s) 366, 605
BspT107I GGYRCC 2 cut(s) 185, 296
BspTNI GGTCTC 1 cut(s) 32
BsrFI RCCGGY 2 cut(s) 173, 506
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 2 cut(s) 173, 506
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 63, 399
BssNI GRCGYC 2 cut(s) 186, 297
BssSI CACGAG 1 cut(s) 202
Bst2BI CACGAG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 20, 562
Bst4CI ACNGT 2 cut(s) 583, 644
Bst6I CTCTTC 2 cut(s) 260, 362
BstACI GRCGYC 2 cut(s) 186, 297
BstAPI GCANNNNNTGC 2 cut(s) 361, 429
BstC8I GCNNGC 3 cut(s) 171, 175, 190
BstDEI CTNAG 1 cut(s) 483
BstF5I GGATG 2 cut(s) 138, 758
BstFNI CGCG 2 cut(s) 198, 761
BstH2I RGCGCY 3 cut(s) 189, 300, 500
BstHHI GCGC 4 cut(s) 188, 299, 499, 761
BstKTI GATC 2 cut(s) 66, 402
BstMAI GTCTC 3 cut(s) 32, 205, 416
BstMBI GATC 2 cut(s) 63, 399
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 7 cut(s) 91, 166, 183, 192, 361, 429, 503
BstNI CCWGG 2 cut(s) 20, 562
BstSCI CCNGG 3 cut(s) 18, 314, 560
BstSFI CTRYAG 2 cut(s) 362, 601
BstUI CGCG 2 cut(s) 198, 761
BstV1I GCAGC 4 cut(s) 179, 195, 348, 743
BstV2I GAAGAC 2 cut(s) 701, 709
Bsu15I ATCGAT 1 cut(s) 56
BsuRI GGCC 4 cut(s) 74, 173, 177, 506
BsuTUI ATCGAT 1 cut(s) 56
BtsCI GGATG 2 cut(s) 138, 758
BtsI GCAGTG 2 cut(s) 362, 610
BtsIMutI CAGTG 2 cut(s) 362, 610
Cac8I GCNNGC 3 cut(s) 171, 175, 190
CaiI CAGNNNCTG 1 cut(s) 361
CfoI GCGC 4 cut(s) 188, 299, 499, 761
Cfr10I RCCGGY 2 cut(s) 173, 506
Cfr13I GGNCC 2 cut(s) 250, 564
ClaI ATCGAT 1 cut(s) 56
CseI GACGC 1 cut(s) 187
CsiI ACCWGGT 1 cut(s) 18
Csp6I GTAC 4 cut(s) 102, 308, 325, 334
CviAII CATG 2 cut(s) 157, 232
CviQI GTAC 4 cut(s) 102, 308, 325, 334
DdeI CTNAG 1 cut(s) 483
DinI GGCGCC 2 cut(s) 187, 298
DpnI GATC 2 cut(s) 65, 401
DpnII GATC 2 cut(s) 63, 399
DrdI GACNNNNNNGTC 2 cut(s) 131, 425
DseDI GACNNNNNNGTC 2 cut(s) 131, 425
EaeI YGGCCR 2 cut(s) 72, 171
Eam1104I CTCTTC 2 cut(s) 260, 362
EarI CTCTTC 2 cut(s) 260, 362
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 2 cut(s) 250, 564
Eco47III AGCGCT 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 485
Eco88I CYCGRG 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 18, 560
EgeI GGCGCC 2 cut(s) 187, 298
EheI GGCGCC 2 cut(s) 187, 298
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 2 cut(s) 160, 235
FaiI YATR 6 cut(s) 41, 82, 158, 233, 453, 677
FaqI GGGAC 2 cut(s) 359, 794
FatI CATG 2 cut(s) 156, 231
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 193, 196, 209, 362, 757
FokI GGATG 2 cut(s) 125, 765
FseI GGCCGGCC 1 cut(s) 177
Fsp4HI GCNGC 5 cut(s) 193, 196, 209, 362, 757
FspBI CTAG 1 cut(s) 122
GlaI GCGC 4 cut(s) 187, 298, 498, 760
GluI GCNGC 5 cut(s) 193, 196, 209, 362, 757
GsuI CTGGAG 1 cut(s) 452
HaeII RGCGCY 3 cut(s) 189, 300, 500
HaeIII GGCC 4 cut(s) 74, 173, 177, 506
HapII CCGG 5 cut(s) 75, 174, 300, 315, 507
HgaI GACGC 1 cut(s) 187
HhaI GCGC 4 cut(s) 188, 299, 499, 761
Hin1I GRCGYC 2 cut(s) 186, 297
Hin1II CATG 2 cut(s) 160, 235
Hin6I GCGC 4 cut(s) 186, 297, 497, 759
HinP1I GCGC 4 cut(s) 186, 297, 497, 759
HincII GTYRAC 2 cut(s) 376, 709
HindII GTYRAC 2 cut(s) 376, 709
HinfI GANTC 1 cut(s) 58
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 5 cut(s) 75, 174, 300, 315, 507
HphI GGTGA 2 cut(s) 256, 277
Hpy166II GTNNAC 3 cut(s) 220, 376, 709
Hpy188I TCNGA 4 cut(s) 63, 484, 526, 592
Hpy188III TCNNGA 3 cut(s) 403, 469, 730
Hpy8I GTNNAC 3 cut(s) 220, 376, 709
HpyAV CCTTC 3 cut(s) 286, 484, 619
HpyCH4III ACNGT 2 cut(s) 583, 644
HpyCH4IV ACGT 2 cut(s) 44, 573
HpyCH4V TGCA 3 cut(s) 364, 603, 654
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 166, 183, 192, 361, 429, 503
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 2 cut(s) 44, 573
Hsp92I GRCGYC 2 cut(s) 186, 297
Hsp92II CATG 2 cut(s) 160, 235
HspAI GCGC 4 cut(s) 186, 297, 497, 759
KasI GGCGCC 2 cut(s) 185, 296
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 2 cut(s) 63, 399
Lsp1109I GCAGC 4 cut(s) 179, 195, 348, 743
LweI GCATC 1 cut(s) 743
MabI ACCWGGT 1 cut(s) 18
MaeI CTAG 1 cut(s) 122
MaeII ACGT 2 cut(s) 44, 573
MaeIII GTNAC 3 cut(s) 262, 283, 574
MalI GATC 2 cut(s) 65, 401
MboI GATC 2 cut(s) 63, 399
MboII GAAGA 7 cut(s) 247, 262, 379, 703, 706, 709, 718
MluCI AATT 2 cut(s) 180, 685
Mly113I GGCGCC 2 cut(s) 186, 297
MmeI TCCRAC 2 cut(s) 615, 730
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 5 cut(s) 75, 174, 300, 315, 507
MspR9I CCNGG 3 cut(s) 20, 316, 562
MvaI CCWGG 2 cut(s) 20, 562
MvnI CGCG 2 cut(s) 198, 761
MwoI GCNNNNNNNGC 7 cut(s) 91, 166, 183, 192, 361, 429, 503
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 2 cut(s) 186, 297
NciI CCSGG 1 cut(s) 316
NdeII GATC 2 cut(s) 63, 399
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 160, 235
NlaIV GGNNCC 2 cut(s) 187, 298
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 262, 283
PcsI WCGNNNNNNNCGW 2 cut(s) 279, 579
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 58
PflMI CCANNNNNTGG 2 cut(s) 390, 561
PfoI TCCNGGA 2 cut(s) 314, 560
PkrI GCNGC 5 cut(s) 194, 197, 210, 363, 758
Ple19I CGATCG 1 cut(s) 66
PluTI GGCGCC 2 cut(s) 189, 300
Psp1406I AACGTT 2 cut(s) 44, 573
Psp6I CCWGG 2 cut(s) 18, 560
PspGI CCWGG 2 cut(s) 18, 560
PspN4I GGNNCC 2 cut(s) 187, 298
PspPI GGNCC 2 cut(s) 250, 564
PstI CTGCAG 2 cut(s) 366, 605
PstNI CAGNNNCTG 1 cut(s) 361
PvuI CGATCG 1 cut(s) 66
RigI GGCCGGCC 1 cut(s) 177
RsaI GTAC 4 cut(s) 103, 309, 326, 335
RsaNI GTAC 4 cut(s) 102, 308, 325, 334
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 5 cut(s) 193, 196, 209, 362, 757
Sau3AI GATC 2 cut(s) 63, 399
Sau96I GGNCC 2 cut(s) 250, 564
ScaI AGTACT 1 cut(s) 309
ScrFI CCNGG 3 cut(s) 20, 316, 562
SetI ASST 9 cut(s) 21, 47, 225, 410, 429, 476, 492, 576, 630
SexAI ACCWGGT 1 cut(s) 18
SfaNI GCATC 1 cut(s) 743
SfcI CTRYAG 2 cut(s) 362, 601
SfoI GGCGCC 2 cut(s) 187, 298
SinI GGWCC 2 cut(s) 250, 564
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 2 cut(s) 180, 685
SsiI CCGC 1 cut(s) 196
SspDI GGCGCC 2 cut(s) 185, 296
SspI AATATT 1 cut(s) 791
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 3 cut(s) 18, 314, 560
TaaI ACNGT 2 cut(s) 583, 644
TaiI ACGT 2 cut(s) 47, 576
TaqI TCGA 1 cut(s) 56
TasI AATT 2 cut(s) 180, 685
TatI WGTACW 2 cut(s) 307, 333
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 362, 610
TseFI GTSAC 2 cut(s) 262, 283
TseI GCWGC 4 cut(s) 192, 208, 361, 756
Tsp45I GTSAC 2 cut(s) 262, 283
TspDTI ATGAA 2 cut(s) 28, 801
TspRI CASTG 2 cut(s) 362, 610
Van91I CCANNNNNTGG 2 cut(s) 390, 561
VpaK11BI GGWCC 2 cut(s) 250, 564
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 1 cut(s) 122
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.