Rh4AG127300

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015286
Physical Location & Seq
Reverse (-)
27565411 .. 27567993
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG127300.1

Sequence Viewer

Length: 804 bp
ATGAGGCTCAGCAACAACCTGGTGGGCTTTCTCAACTTTCTGACCTTCCTCTGCTCCATCCCGATCGTCGTCGCCGGAGTATGGCTGAGCAAGCAAGGCAACACCGAGTGCGAGAAGTTTCTGGAGAAGCCGATCATCATCCTCGGCATCTTCCTCATGTTCGTCTCCCTCGCCGGCTTCATCGGCTCCTGTTTCCGCGTCTCCTGGCTCCTCTGGCTCTACCTCCTCATCATGTTCCTCCTCATCGTCGTCCTCTTCGGCTACACCATCTTCGCCTTCGTAGTCACCAACAAGGGCGCCGGAACCGTGCTCTCCGGTAAGGGATACAAGGAGTACAAGCTCGGTGACTACTCTCACTGGCTGCAGAAGAGAGTCAACAGCACCAAGAACTGGAACAAGATCAGGAGCTGCCTCAGTGATAGCAAGGTCTGCTCCAGTTTCCAGGACAAGTATCTCAATGATACTCTCCCCCTTCTTTATACCGAGAACTTGTCTGCGCTTCAGGCTGGCTGCTGTAAGCCATCAGATGAATGTGGTTTTGCTTATGAAAGTCCCACTGTCTGGACCAAGAATGCTACTGTTGTATCTACCAACCCTGACTGCAATGCATGGGATAATGATCCAAAGGTTCTGTGCTTCAACTGCCAGTCATGTAAGGCTGGACTGCTCGACAATCTGAAAAGTAACTGGAAGAAGACGGCTGTTGTCAACATTGTCTTCCTTATCTTCCTCATCATCGTGTATTCCATAGGGTGCTGTGCATTCAGGAATAACAGGAGGGACAATGAATATTATAAGCAGTAA

Protein Analysis

267

Amino Acids

30.26

Weight (kDa)

8.86

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 254 7.6e-40 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23810
fragaria_vesca FvH4_4g10210
malus_domestica MD13G1273700.v1.1 MD16G1261500.v1.1
prunus_persica Prupe.1G098200_v2.0.a1
pyrus_communis pycom13g28840 pycom16g22990
rosa_chinensis RchiOBHm_Chr4g0405031
rosa_laevigata RLG00000008883
rosa_multiflora Rmu_ssc0000127.1_g000047
rosa_roxburghii Rroxscaffold_5G00349210
rosa_rugosa Rorug04G0052400
rosa_samantha Rh4AG127300 Rh4BG121200 Rh4CG135300
rosa_wichuraiana Rw4G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AccB1I GGYRCC 1 cut(s) 296
AccB7I CCANNNNNTGG 2 cut(s) 390, 561
AccII CGCG 1 cut(s) 198
AciI CCGC 1 cut(s) 196
AclWI GGATC 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 108
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 3 cut(s) 81, 390, 561
AgsI TTSAA 1 cut(s) 640
AjnI CCWGG 3 cut(s) 18, 203, 441
AluBI AGCT 2 cut(s) 340, 408
AluI AGCT 2 cut(s) 340, 408
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 2 cut(s) 169, 205
AlwI GGATC 1 cut(s) 614
AlwNI CAGNNNCTG 1 cut(s) 408
ApeKI GCWGC 3 cut(s) 361, 408, 510
AspLEI GCGC 2 cut(s) 299, 499
AspS9I GGNCC 1 cut(s) 564
AsuHPI GGTGA 2 cut(s) 277, 356
AvaII GGWCC 1 cut(s) 564
BaeI ACNNNNGTAYC 2 cut(s) 567, 600
BanI GGYRCC 1 cut(s) 296
BbsI GAAGAC 2 cut(s) 701, 709
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 348, 395, 497
BccI CCATC 3 cut(s) 65, 275, 529
BceAI ACGGC 1 cut(s) 714
BciT130I CCWGG 3 cut(s) 20, 205, 443
BciVI GTATCC 1 cut(s) 317
BcoDI GTCTC 2 cut(s) 169, 205
BfmI CTRYAG 1 cut(s) 362
BfoI RGCGCY 1 cut(s) 300
BfuI GTATCC 1 cut(s) 317
BisI GCNGC 3 cut(s) 362, 409, 511
BlpI GCTNAGC 2 cut(s) 8, 86
BlsI GCNGC 3 cut(s) 363, 410, 512
Bme1390I CCNGG 3 cut(s) 20, 205, 443
Bme18I GGWCC 1 cut(s) 564
BmgT120I GGNCC 1 cut(s) 564
BmiI GGNNCC 4 cut(s) 187, 209, 298, 304
BmrFI CCNGG 3 cut(s) 20, 205, 443
BmsI GCATC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 701, 709
BpmI CTGGAG 2 cut(s) 143, 418
Bpu1102I GCTNAGC 2 cut(s) 8, 86
BsaBI GATNNNNATC 2 cut(s) 137, 618
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 390, 561
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 5 cut(s) 362, 395, 435, 646, 692
Bse3DI GCAATG 1 cut(s) 610
Bse8I GATNNNNATC 2 cut(s) 137, 618
BseBI CCWGG 3 cut(s) 20, 205, 443
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 57, 138
BseJI GATNNNNATC 2 cut(s) 137, 618
BseLI CCNNNNNNNGG 3 cut(s) 81, 390, 561
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 3 cut(s) 22, 77, 427
BseNI ACTGG 5 cut(s) 362, 395, 435, 646, 692
BseRI GAGGAG 3 cut(s) 200, 215, 230
BseXI GCAGC 3 cut(s) 348, 395, 497
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 66
BshNI GGYRCC 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 4 cut(s) 75, 174, 300, 315
BslFI GGGAC 2 cut(s) 537, 794
BslI CCNNNNNNNGG 3 cut(s) 81, 390, 561
BsmAI GTCTC 2 cut(s) 169, 205
BsmBI CGTCTC 2 cut(s) 169, 205
BsmFI GGGAC 2 cut(s) 537, 794
BsmI GAATGC 2 cut(s) 577, 761
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 4 cut(s) 63, 132, 399, 619
Bsp1720I GCTNAGC 2 cut(s) 8, 86
BspACI CCGC 1 cut(s) 196
BspCNI CTCAG 3 cut(s) 21, 78, 426
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 4 cut(s) 187, 209, 298, 304
BspMAI CTGCAG 1 cut(s) 366
BspPI GGATC 1 cut(s) 614
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 610
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 5 cut(s) 362, 395, 435, 646, 692
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 4 cut(s) 63, 132, 399, 619
BssNI GRCGYC 1 cut(s) 297
Bst2UI CCWGG 3 cut(s) 20, 205, 443
Bst4CI ACNGT 3 cut(s) 307, 559, 580
Bst6I CTCTTC 2 cut(s) 260, 362
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 92, 175, 508
BstDEI CTNAG 3 cut(s) 8, 86, 413
BstF5I GGATG 2 cut(s) 57, 138
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 2 cut(s) 299, 499
BstKTI GATC 4 cut(s) 66, 135, 402, 622
BstMAI GTCTC 2 cut(s) 169, 205
BstMBI GATC 4 cut(s) 63, 132, 399, 619
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 7 cut(s) 91, 96, 183, 214, 429, 503, 642
BstNI CCWGG 3 cut(s) 20, 205, 443
BstSCI CCNGG 3 cut(s) 18, 203, 441
BstSFI CTRYAG 1 cut(s) 362
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 348, 395, 497
BstV2I GAAGAC 2 cut(s) 701, 709
BsuI GTATCC 1 cut(s) 317
BtsCI GGATG 2 cut(s) 57, 138
BtsIMutI CAGTG 3 cut(s) 355, 421, 555
Cac8I GCNNGC 3 cut(s) 92, 175, 508
CaiI CAGNNNCTG 1 cut(s) 408
CfoI GCGC 2 cut(s) 299, 499
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 564
CseI GACGC 1 cut(s) 187
CsiI ACCWGGT 1 cut(s) 18
Csp6I GTAC 1 cut(s) 334
CviAII CATG 4 cut(s) 157, 232, 609, 651
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 3 cut(s) 8, 86, 413
DinI GGCGCC 1 cut(s) 298
DpnI GATC 4 cut(s) 65, 134, 401, 621
DpnII GATC 4 cut(s) 63, 132, 399, 619
DraIII CACNNNGTG 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 260, 362
EarI CTCTTC 2 cut(s) 260, 362
Eco47I GGWCC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 485
EcoRII CCWGG 3 cut(s) 18, 203, 441
EcoT22I ATGCAT 1 cut(s) 610
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
Esp3I CGTCTC 2 cut(s) 169, 205
FaeI CATG 4 cut(s) 160, 235, 612, 654
FaiI YATR 9 cut(s) 82, 158, 233, 480, 546, 610, 652, 749, 795
FaqI GGGAC 2 cut(s) 537, 794
FatI CATG 4 cut(s) 156, 231, 608, 650
Fnu4HI GCNGC 3 cut(s) 362, 409, 511
FokI GGATG 2 cut(s) 44, 125
Fsp4HI GCNGC 3 cut(s) 362, 409, 511
GlaI GCGC 2 cut(s) 298, 498
GluI GCNGC 3 cut(s) 362, 409, 511
GsuI CTGGAG 2 cut(s) 143, 418
HaeII RGCGCY 1 cut(s) 300
HapII CCGG 4 cut(s) 75, 174, 300, 315
HgaI GACGC 1 cut(s) 187
HhaI GCGC 2 cut(s) 299, 499
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 4 cut(s) 160, 235, 612, 654
Hin6I GCGC 2 cut(s) 297, 497
HinP1I GCGC 2 cut(s) 297, 497
HincII GTYRAC 2 cut(s) 376, 709
HindII GTYRAC 2 cut(s) 376, 709
HinfI GANTC 1 cut(s) 372
HpaII CCGG 4 cut(s) 75, 174, 300, 315
HphI GGTGA 2 cut(s) 277, 356
Hpy166II GTNNAC 2 cut(s) 376, 709
Hpy188I TCNGA 3 cut(s) 42, 526, 678
Hpy188III TCNNGA 5 cut(s) 61, 122, 403, 562, 766
Hpy8I GTNNAC 2 cut(s) 376, 709
Hpy99I CGWCG 3 cut(s) 71, 74, 251
HpyAV CCTTC 3 cut(s) 55, 286, 482
HpyCH4III ACNGT 3 cut(s) 307, 559, 580
HpyCH4V TGCA 4 cut(s) 364, 603, 608, 761
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 96, 183, 214, 429, 503, 642
HpyF3I CTNAG 3 cut(s) 8, 86, 413
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 4 cut(s) 160, 235, 612, 654
HspAI GCGC 2 cut(s) 297, 497
KasI GGCGCC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 4 cut(s) 63, 132, 399, 619
LmnI GCTCC 5 cut(s) 59, 191, 213, 405, 437
Lsp1109I GCAGC 3 cut(s) 348, 395, 497
LweI GCATC 1 cut(s) 156
MabI ACCWGGT 1 cut(s) 18
MaeIII GTNAC 3 cut(s) 283, 344, 683
MalI GATC 4 cut(s) 65, 134, 401, 621
MboI GATC 4 cut(s) 63, 132, 399, 619
MboII GAAGA 8 cut(s) 142, 247, 262, 379, 703, 706, 709, 718
MhlI GDGCHC 1 cut(s) 312
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 381
Mph1103I ATGCAT 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 737
MspI CCGG 4 cut(s) 75, 174, 300, 315
MspR9I CCNGG 3 cut(s) 20, 205, 443
Mva1269I GAATGC 2 cut(s) 577, 761
MvaI CCWGG 3 cut(s) 20, 205, 443
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 7 cut(s) 91, 96, 183, 214, 429, 503, 642
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 1 cut(s) 297
NdeII GATC 4 cut(s) 63, 132, 399, 619
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 4 cut(s) 160, 235, 612, 654
NlaIV GGNNCC 4 cut(s) 187, 209, 298, 304
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 283, 344
NsiI ATGCAT 1 cut(s) 610
PctI GAATGC 2 cut(s) 577, 761
PdiI GCCGGC 1 cut(s) 175
PflMI CCANNNNNTGG 2 cut(s) 390, 561
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 3 cut(s) 363, 410, 512
Ple19I CGATCG 1 cut(s) 66
PleI GAGTC 1 cut(s) 380
PluTI GGCGCC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 380
PsiI TTATAA 1 cut(s) 795
Psp6I CCWGG 3 cut(s) 18, 203, 441
PspGI CCWGG 3 cut(s) 18, 203, 441
PspN4I GGNNCC 4 cut(s) 187, 209, 298, 304
PspPI GGNCC 1 cut(s) 564
PstI CTGCAG 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 408
PvuI CGATCG 1 cut(s) 66
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 737
SatI GCNGC 3 cut(s) 362, 409, 511
Sau3AI GATC 4 cut(s) 63, 132, 399, 619
Sau96I GGNCC 1 cut(s) 564
SchI GAGTC 1 cut(s) 381
ScrFI CCNGG 3 cut(s) 20, 205, 443
SduI GDGCHC 1 cut(s) 312
SetI ASST 7 cut(s) 21, 47, 225, 342, 410, 429, 630
SexAI ACCWGGT 1 cut(s) 18
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 362
SfoI GGCGCC 1 cut(s) 298
SinI GGWCC 1 cut(s) 564
SmiMI CAYNNNNRTG 1 cut(s) 737
SsiI CCGC 1 cut(s) 196
SspDI GGCGCC 1 cut(s) 296
SspI AATATT 1 cut(s) 791
StyD4I CCNGG 3 cut(s) 18, 203, 441
TaaI ACNGT 3 cut(s) 307, 559, 580
TaqI TCGA 1 cut(s) 669
TatI WGTACW 1 cut(s) 333
TscAI CASTG 3 cut(s) 362, 421, 562
TseFI GTSAC 2 cut(s) 283, 344
TseI GCWGC 3 cut(s) 361, 408, 510
Tsp45I GTSAC 2 cut(s) 283, 344
TspDTI ATGAA 4 cut(s) 169, 543, 561, 801
TspRI CASTG 3 cut(s) 362, 421, 562
Van91I CCANNNNNTGG 2 cut(s) 390, 561
VpaK11BI GGWCC 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.