MD16G1262100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006771
Physical Location & Seq
Forward (+)
32573050 .. 32576083
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1262100.v1.1.491

Sequence Viewer

Length: 531 bp
ATGATCAACAAAGTGATACACTATCTTACTTCTGACACTTACATGTTTGCGCCTCTAATCTCCCACCCACCTCACGCCTTTCGCTCCCCCATAAAGACACCGACTTCTTCTTCTTCTTCTTCTTCTACTTCAGGATTGCAAGCAAGGCAACCCATCGAAGGAAACAGCAAGAGGTTGCTCAACAATGTTGGGGACTATCTCAAGTCTGACTCTTACATGTATGCTCCCCTGCTTGTTCCGCCACCACAAACCATTACTAGTACTACGAGCCCTCCAAAAGGGTCGTCTGAGTACCTTGAGAGGGTTGTAACCATGGCAGTTTCGAAGAAGAAATTAATTTTCATAGACAACAAATCAAATGAGCCAAATGATCAGCCGTGTGCAAAGGCAATAGCACGGAATGATGCCTCCAAGGGCCACCGTCCTAAAAAAGTCATTTCAGAGAAGCAGTCTGTAGTGCACAAGGAAACAGTAAAGCACGTGGTTCCCAATAGTTGTCGCTCATCGTTGATGTCAGGTCACCTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.36

Weight (kDa)

9.78

Isoelectric Point (pI)

71.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 114
AcvI CACGTG 1 cut(s) 481
AfaI GTAC 2 cut(s) 262, 293
AfiI CCNNNNNNNGG 3 cut(s) 158, 278, 301
AflIII ACRYGT 2 cut(s) 42, 216
AhlI ACTAGT 1 cut(s) 257
Alw21I GWGCWC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 458
AoxI GGCC 1 cut(s) 415
ApaLI GTGCAC 1 cut(s) 458
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AseI ATTAAT 1 cut(s) 335
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 512
AsuII TTCGAA 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 462
BanII GRGCYC 1 cut(s) 272
BbrPI CACGTG 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 462
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 361
BclI TGATCA 2 cut(s) 3, 370
BcuI ACTAGT 1 cut(s) 257
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 453
BmcAI AGTACT 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 486
BmsI GCATC 1 cut(s) 394
Bpu14I TTCGAA 1 cut(s) 323
BpuEI CTTGAG 2 cut(s) 185, 317
BsaAI YACGTR 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 312, 411
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 278, 301
BseDI CCNNGG 2 cut(s) 312, 411
BseLI CCNNNNNNNGG 3 cut(s) 158, 278, 301
BseMII CTCAG 1 cut(s) 279
BseSI GKGCMC 1 cut(s) 462
BshFI GGCC 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 462
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 3 cut(s) 158, 278, 301
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 1 cut(s) 417
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 2 cut(s) 272, 462
Bsp143I GATC 2 cut(s) 3, 370
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 417
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 486
BspT104I TTCGAA 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 312, 411
BssMI GATC 2 cut(s) 3, 370
BssT1I CCWWGG 2 cut(s) 312, 411
Bst4CI ACNGT 2 cut(s) 422, 472
BstBAI YACGTR 1 cut(s) 481
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 312
BstEII GGTNACC 1 cut(s) 518
BstENI CCTNNNNNAGG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 2 cut(s) 6, 373
BstMBI GATC 2 cut(s) 3, 370
BstMWI GCNNNNNNNGC 2 cut(s) 145, 238
BstNSI RCATGY 2 cut(s) 46, 220
BstPI GGTNACC 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 462
BsuRI GGCC 1 cut(s) 417
BtgI CCRYGG 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 141
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 2 cut(s) 261, 292
CviAII CATG 3 cut(s) 43, 217, 313
CviJI RGCY 4 cut(s) 270, 364, 376, 417
CviKI_1 RGCY 4 cut(s) 270, 364, 376, 417
CviQI GTAC 2 cut(s) 261, 292
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 5, 372
DpnII GATC 2 cut(s) 3, 370
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 312, 411
Eco24I GRGCYC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 114
Eco72I CACGTG 1 cut(s) 481
Eco91I GGTNACC 1 cut(s) 518
EcoNI CCTNNNNNAGG 1 cut(s) 276
EcoO65I GGTNACC 1 cut(s) 518
EcoT14I CCWWGG 2 cut(s) 312, 411
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 2 cut(s) 312, 411
FaeI CATG 3 cut(s) 46, 220, 316
FaiI YATR 6 cut(s) 44, 92, 218, 222, 314, 344
FaqI GGGAC 1 cut(s) 206
FatI CATG 3 cut(s) 42, 216, 312
FbaI TGATCA 2 cut(s) 3, 370
FriOI GRGCYC 1 cut(s) 272
FspBI CTAG 1 cut(s) 258
GlaI GCGC 1 cut(s) 51
HaeIII GGCC 1 cut(s) 417
HhaI GCGC 1 cut(s) 52
Hin1II CATG 3 cut(s) 46, 220, 316
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 1 cut(s) 209
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 4 cut(s) 34, 208, 289, 442
Hpy188III TCNNGA 1 cut(s) 132
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 2 cut(s) 422, 472
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 3 cut(s) 139, 383, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 238
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 3 cut(s) 46, 220, 316
HspAI GCGC 1 cut(s) 50
Ksp22I TGATCA 2 cut(s) 3, 370
Kzo9I GATC 2 cut(s) 3, 370
LmnI GCTCC 2 cut(s) 89, 229
LpnPI CCDG 3 cut(s) 117, 242, 501
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 2 cut(s) 307, 518
MalI GATC 2 cut(s) 5, 372
MboI GATC 2 cut(s) 3, 370
MboII GAAGA 8 cut(s) 99, 102, 105, 108, 111, 114, 337, 340
MhlI GDGCHC 2 cut(s) 272, 462
MluCI AATT 2 cut(s) 332, 336
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 6 cut(s) 63, 81, 165, 282, 294, 418
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 145, 238
NcoI CCATGG 1 cut(s) 312
NdeII GATC 2 cut(s) 3, 370
NlaIII CATG 3 cut(s) 46, 220, 316
NlaIV GGNNCC 1 cut(s) 486
NmuCI GTSAC 1 cut(s) 518
NspI RCATGY 2 cut(s) 46, 220
NspV TTCGAA 1 cut(s) 323
PciI ACATGT 2 cut(s) 42, 216
PleI GAGTC 1 cut(s) 203
PmaCI CACGTG 1 cut(s) 481
PmlI CACGTG 1 cut(s) 481
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 481
PscI ACATGT 2 cut(s) 42, 216
PshBI ATTAAT 1 cut(s) 335
PspCI CACGTG 1 cut(s) 481
PspEI GGTNACC 1 cut(s) 518
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 1 cut(s) 415
RsaI GTAC 2 cut(s) 262, 293
RsaNI GTAC 2 cut(s) 261, 292
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 335
Sau3AI GATC 2 cut(s) 3, 370
Sau96I GGNCC 1 cut(s) 415
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 2 cut(s) 272, 462
SetI ASST 6 cut(s) 73, 176, 297, 483, 520, 525
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 453
SfuI TTCGAA 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 200, 296
SmoI CTYRAG 2 cut(s) 200, 296
SpeI ACTAGT 1 cut(s) 257
Sse9I AATT 2 cut(s) 332, 336
SsiI CCGC 1 cut(s) 239
SspMI CTAG 1 cut(s) 258
StyI CCWWGG 2 cut(s) 312, 411
TaaI ACNGT 2 cut(s) 422, 472
TaiI ACGT 1 cut(s) 483
TaqI TCGA 2 cut(s) 156, 323
TasI AATT 2 cut(s) 332, 336
TatI WGTACW 1 cut(s) 260
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TseFI GTSAC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 1 cut(s) 331
TspGWI ACGGA 1 cut(s) 412
VneI GTGCAC 1 cut(s) 458
VspI ATTAAT 1 cut(s) 335
XagI CCTNNNNNAGG 1 cut(s) 276
XceI RCATGY 2 cut(s) 46, 220
XspI CTAG 1 cut(s) 258
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.