pycom16g23030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006771
Physical Location & Seq
Forward (+)
23557134 .. 23560040
2907 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g23030.4

Sequence Viewer

Length: 642 bp
ATGACGCAGAGCAAAGTGTTGGTAGGGTTAGGAGTCCAGAAGAAAAGAACCATTAAGCGTAATAAGAGGAAGAAAATGATCAACAAAGTGATACACTATCTCACTTCTGACACTTACATGTTTGCGCCTCTAATCTCCCATCCACCTCACTCCTTTGGCTCCCCCATAAAGACACCGACTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTACTTTAGGTAGATGGTATTTATGTCCTCATGATTGGAAGCAAGGCAACCCATCGAAGGAAACAGCAAGAGGTTGCTCAACAATGTTGGGGACTATCTCAAGTCTGACTCTTACATGTATGCTCCCCTGCTTGCTCCACCACCGCAAACCAGCACTAGTACTACGAGCCCTCCAAAAGAAATGGTCGTCTGAGTACCTTGAGAGGGTTGTAACCATGGCAGTTTCGAATAAGAAATTAATTTTCAAAGACAAGAAATCAAATGAACCAAATTATCAGCCGTGTGCAAAAGCGATAGCACGGAACGAAGCCTCCAAGGGCCACCGTCCTGAAAATGTCATTTCAGAGAAGCAGTCTGTAGTGCACAAGGAAACAGTAAAGCACGTGGTTCCCAGTAGTTGTTGCTCATCGTTGATGTCAGGTCACCTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

23.79

Weight (kDa)

10.01

Isoelectric Point (pI)

64.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 352
AcvI CACGTG 1 cut(s) 592
AfaI GTAC 3 cut(s) 211, 369, 404
AfiI CCNNNNNNNGG 2 cut(s) 265, 412
AflIII ACRYGT 2 cut(s) 117, 323
AgsI TTSAA 1 cut(s) 454
AhlI ACTAGT 1 cut(s) 364
AloI GAACNNNNNNTCC 2 cut(s) 503, 535
Alw21I GWGCWC 1 cut(s) 573
Alw44I GTGCAC 1 cut(s) 569
AoxI GGCC 1 cut(s) 526
ApaLI GTGCAC 1 cut(s) 569
AseI ATTAAT 1 cut(s) 446
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 623
AsuII TTCGAA 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 573
BanII GRGCYC 1 cut(s) 379
BbrPI CACGTG 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 573
BccI CCATC 3 cut(s) 147, 216, 268
BceAI ACGGC 1 cut(s) 472
BclI TGATCA 1 cut(s) 78
BcuI ACTAGT 1 cut(s) 364
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 564
BmcAI AGTACT 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 2 cut(s) 160, 597
BmrI ACTGGG 1 cut(s) 594
BmuI ACTGGG 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 434
BpuEI CTTGAG 2 cut(s) 292, 428
BsaAI YACGTR 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 423, 522
BsaXI ACNNNNNCTCC 4 cut(s) 363, 393, 503, 533
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 412
Bse1I ACTGG 1 cut(s) 600
BseDI CCNNGG 2 cut(s) 423, 522
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 2 cut(s) 265, 412
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 573
BshFI GGCC 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 573
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 2 cut(s) 265, 412
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 1 cut(s) 528
Bsp119I TTCGAA 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 379, 573
Bsp143I GATC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 1 cut(s) 352
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 1 cut(s) 391
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 160, 597
BspT104I TTCGAA 1 cut(s) 434
BsrI ACTGG 1 cut(s) 600
BssECI CCNNGG 2 cut(s) 423, 522
BssMI GATC 1 cut(s) 78
BssT1I CCWWGG 2 cut(s) 423, 522
Bst4CI ACNGT 2 cut(s) 533, 583
BstBAI YACGTR 1 cut(s) 592
BstBI TTCGAA 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 1 cut(s) 399
BstDSI CCRYGG 1 cut(s) 423
BstEII GGTNACC 1 cut(s) 629
BstF5I GGATG 1 cut(s) 139
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 1 cut(s) 81
BstMBI GATC 1 cut(s) 78
BstNSI RCATGY 2 cut(s) 121, 327
BstPI GGTNACC 1 cut(s) 629
BstSFI CTRYAG 1 cut(s) 564
BstSLI GKGCMC 1 cut(s) 573
BsuRI GGCC 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 341
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 526
CseI GACGC 1 cut(s) 13
Csp6I GTAC 3 cut(s) 210, 368, 403
CviAII CATG 4 cut(s) 118, 239, 324, 424
CviJI RGCY 5 cut(s) 159, 377, 487, 518, 528
CviKI_1 RGCY 5 cut(s) 159, 377, 487, 518, 528
CviQI GTAC 3 cut(s) 210, 368, 403
DdeI CTNAG 1 cut(s) 399
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
Eco130I CCWWGG 2 cut(s) 423, 522
Eco24I GRGCYC 1 cut(s) 379
Eco72I CACGTG 1 cut(s) 592
Eco91I GGTNACC 1 cut(s) 629
EcoO65I GGTNACC 1 cut(s) 629
EcoT14I CCWWGG 2 cut(s) 423, 522
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 423, 522
FaeI CATG 4 cut(s) 121, 242, 327, 427
FaiI YATR 7 cut(s) 119, 167, 232, 240, 325, 329, 425
FaqI GGGAC 1 cut(s) 313
FatI CATG 4 cut(s) 117, 238, 323, 423
FbaI TGATCA 1 cut(s) 78
FokI GGATG 1 cut(s) 126
FriOI GRGCYC 1 cut(s) 379
FspBI CTAG 1 cut(s) 365
GlaI GCGC 1 cut(s) 126
HaeIII GGCC 1 cut(s) 528
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 127
Hin1II CATG 4 cut(s) 121, 242, 327, 427
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HinfI GANTC 2 cut(s) 33, 316
HphI GGTGA 1 cut(s) 623
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 4 cut(s) 109, 315, 400, 553
Hpy188III TCNNGA 3 cut(s) 37, 239, 536
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 2 cut(s) 533, 583
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 2 cut(s) 494, 571
HpyF3I CTNAG 1 cut(s) 399
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 4 cut(s) 121, 242, 327, 427
HspAI GCGC 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 3 cut(s) 164, 336, 348
LpnPI CCDG 6 cut(s) 50, 349, 372, 549, 612, 613
MaeI CTAG 1 cut(s) 365
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 418, 629
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MhlI GDGCHC 2 cut(s) 379, 573
MluCI AATT 3 cut(s) 443, 447, 478
MlyI GAGTC 2 cut(s) 42, 310
MnlI CCTC 8 cut(s) 60, 138, 156, 246, 272, 389, 405, 529
MseI TTAA 2 cut(s) 54, 446
MslI CAYNNNNRTG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 423
NdeII GATC 1 cut(s) 78
NlaIII CATG 4 cut(s) 121, 242, 327, 427
NlaIV GGNNCC 2 cut(s) 160, 597
NmuCI GTSAC 1 cut(s) 629
NspI RCATGY 2 cut(s) 121, 327
NspV TTCGAA 1 cut(s) 434
PagI TCATGA 1 cut(s) 238
PciI ACATGT 2 cut(s) 117, 323
PleI GAGTC 2 cut(s) 41, 310
PmaCI CACGTG 1 cut(s) 592
PmlI CACGTG 1 cut(s) 592
PpsI GAGTC 2 cut(s) 41, 310
Ppu21I YACGTR 1 cut(s) 592
PscI ACATGT 2 cut(s) 117, 323
PshBI ATTAAT 1 cut(s) 446
PspCI CACGTG 1 cut(s) 592
PspEI GGTNACC 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 160, 597
PspPI GGNCC 1 cut(s) 526
RsaI GTAC 3 cut(s) 211, 369, 404
RsaNI GTAC 3 cut(s) 210, 368, 403
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 2 cut(s) 54, 446
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 1 cut(s) 526
ScaI AGTACT 1 cut(s) 369
SchI GAGTC 2 cut(s) 42, 310
SduI GDGCHC 2 cut(s) 379, 573
SetI ASST 7 cut(s) 148, 220, 283, 408, 594, 631, 636
SfcI CTRYAG 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 307, 407
SmoI CTYRAG 2 cut(s) 307, 407
SpeI ACTAGT 1 cut(s) 364
Sse9I AATT 3 cut(s) 443, 447, 478
SsiI CCGC 1 cut(s) 352
SspMI CTAG 1 cut(s) 365
StyI CCWWGG 2 cut(s) 423, 522
TaaI ACNGT 2 cut(s) 533, 583
TaiI ACGT 1 cut(s) 594
TaqI TCGA 2 cut(s) 263, 434
TasI AATT 3 cut(s) 443, 447, 478
TatI WGTACW 2 cut(s) 209, 367
Tru1I TTAA 2 cut(s) 54, 446
Tru9I TTAA 2 cut(s) 54, 446
TseFI GTSAC 1 cut(s) 629
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 523
VneI GTGCAC 1 cut(s) 569
VspI ATTAAT 1 cut(s) 446
XceI RCATGY 2 cut(s) 121, 327
XspI CTAG 1 cut(s) 365
ZrmI AGTACT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.