MD16G1263000.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012281
Physical Location & Seq
Forward (+)
32907522 .. 32908781
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1263000.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGGTATGGATGTGGAAGCTCCTTGAAATCAAAGAAAATGGGGTTTCTTCAATTCCCACGTTCCCACCATACTTTGTTCCTCAGCTTCCTCCCCCACCCCCAAATCTCCAATCAAATAGTTTGAGTTTTGGGAACAAAGTCAGTCCAAGCATACTGCTAATAGTCATAATAGTAGCCATTGTTTTCTTTGTTGCTGTGCTGCTCCACCTCCTTGTTAGATTCCTCCTGAGGCCTCCAGCCAGAGATTCAGAGGACTTGGAGAGTGTGACAGTTCTCCAAGGGCAACTGCAGCAACTGTTCCGCCTCCATGATGCAGGCGTCGACCAGTCCTTCATAGACGCCCTCCCTGTCTTCTACTACAAAGCCATCATTGGATTGAAAAACCCTTTTGATTGTGCTGTCTGTTTGTGTGAGTTTGAGGCTGAGGATAACCTCAGATTGCTCCCCAAATGCAGCCATGCCTTCCACATGAAGTGCATTGACACTTGGCTCTTGTCTCACTCCAGTTGCCCTCTCTGTAGAGCTACTTTGCTCCCTGATTTCTCACGCAGCAATGGCTGCTCACCCATTGTTTTTGTTCTTGAATCTGGGAGTGACAGCTCCAGAGAGATGGTCTCTGATAGAGATGGACTCACTTCCCATCTGGGTTTTCATGGAGACATGGAATTGGGGTTGTCATACAAATCTTGCGAAATTGTGGCGAAAAATGAGGCTAACACGGCGGTGATGGTGGATTCCGGGAAAAAGGTAGTGCCTGTGAAGCTTGGGAAGTTCAGAAATGTGGATAGTGGGGGTGAGGGGAGTAGTGACAGCAATGTGGATTCAAGGAGGTGCTTTTCTATGGGCTCATTTGCGTATGTGATGGATGACAAATATGAATTGCGAGTACCGATTAGAGCCTCGATGAAAAGGCAAGCAATGTCGGAATGCGATTGTGAGTCGAGGAGAGAATTCAAGTTTAGTGGGGTCGAAGCAATTGGAAGCCTTGAAACTCAAGGAACTAGTACTGGCGCTAGTTGTACTAATGGCAATGGCAGTAATGGCATTGGGAGGAGCATGCGGGAGAGCTTCTCGATATCGAAGATTTGGCTTATGGGGAAGAAGGAGAAGCCAAATGGGGCGGCAGATTCGTCACGAAGGGCCTTTTCGTTTCGGTTTCCAGTGCACCAGAGTGCGGTGGCGGCAGGGGAGGGAGCGAAGGCCAAGAATGTTGGGAGTGAGAGTAGGAGGACAATTTCAGAGATTGAAATTGGGAGGTAG

Protein Analysis

420

Amino Acids

46.0

Weight (kDa)

7.51

Isoelectric Point (pI)

53.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 127 - 174 1.1e-09 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 129 - 176 8.3e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 131 - 174 6.9e-14 Ring finger domain
zf-RING_11 PF17123 131 - 160 3.2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 132 - 173 7.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18650 AT4G30400
fragaria_vesca FvH4_4g09580 FvH4_4g09580
malus_domestica MD13G1260900.v1.1 MD16G1263000.v1.1
prunus_persica Prupe.1G097100_v2.0.a1
pyrus_communis pycom16g23190
rosa_chinensis RchiOBHm_Chr4g0404881
rosa_laevigata RLG00000008897
rosa_multiflora Rmu_sc0001179.1_g000015
rosa_roxburghii Rroxscaffold_5G00349010
rosa_rugosa Rorug04G0049700 Rorug04G0049700
rosa_samantha Rh4AG125700 Rh4BG119500 Rh4CG133900 Rh4DG119300
rosa_wichuraiana Rw4G010170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AciI CCGC 6 cut(s) 301, 722, 1060, 1121, 1175, 1181
AcsI RAATTY 1 cut(s) 950
AcyI GRCGYC 2 cut(s) 318, 339
AfaI GTAC 3 cut(s) 888, 1006, 1021
AfiI CCNNNNNNNGG 1 cut(s) 1174
AgsI TTSAA 8 cut(s) 26, 51, 379, 584, 825, 955, 989, 1247
AhlI ACTAGT 1 cut(s) 1001
AleI CACNNNNGTG 2 cut(s) 722, 1170
AluBI AGCT 6 cut(s) 19, 85, 524, 600, 763, 1068
AluI AGCT 6 cut(s) 19, 85, 524, 600, 763, 1068
Alw21I GWGCWC 1 cut(s) 1167
Alw26I GTCTC 3 cut(s) 501, 619, 651
Alw44I GTGCAC 1 cut(s) 1163
AlwNI CAGNNNCTG 1 cut(s) 295
AoxI GGCC 3 cut(s) 230, 1140, 1200
ApaLI GTGCAC 1 cut(s) 1163
ApeKI GCWGC 5 cut(s) 199, 289, 453, 549, 558
ApoI RAATTY 1 cut(s) 950
AspLEI GCGC 1 cut(s) 1013
AspS9I GGNCC 1 cut(s) 1140
AsuC2I CCSGG 1 cut(s) 739
AsuHPI GGTGA 3 cut(s) 555, 736, 806
AxyI CCTNAGG 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 1167
BanII GRGCYC 1 cut(s) 848
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 1167
BbvCI CCTCAGC 2 cut(s) 81, 423
BbvI GCAGC 5 cut(s) 186, 301, 465, 545, 561
BccI CCATC 6 cut(s) 374, 604, 620, 648, 721, 856
BceAI ACGGC 1 cut(s) 735
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 501, 619, 651
BcuI ACTAGT 1 cut(s) 1001
BfaI CTAG 2 cut(s) 1002, 1014
BfmI CTRYAG 2 cut(s) 287, 517
BfoI RGCGCY 1 cut(s) 1014
BisI GCNGC 7 cut(s) 200, 290, 454, 550, 559, 1122, 1182
BlsI GCNGC 7 cut(s) 201, 291, 455, 551, 560, 1123, 1183
BmcAI AGTACT 1 cut(s) 1006
Bme1390I CCNGG 1 cut(s) 739
BmgT120I GGNCC 1 cut(s) 1140
BmrFI CCNGG 1 cut(s) 739
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 343
BplI GAGNNNNNCTC 6 cut(s) 599, 631, 615, 647, 1055, 1087
BpmI CTGGAG 3 cut(s) 219, 487, 586
Bpu10I CCTNAGC 2 cut(s) 81, 423
BpuEI CTTGAG 1 cut(s) 978
BpuMI CCSGG 1 cut(s) 739
BsaHI GRCGYC 2 cut(s) 318, 339
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 1207, 1237
Bsc4I CCNNNNNNNGG 1 cut(s) 1174
Bse1I ACTGG 4 cut(s) 325, 504, 1012, 1160
Bse21I CCTNAGG 1 cut(s) 227
Bse3DI GCAATG 4 cut(s) 559, 820, 924, 1036
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 2 cut(s) 15, 871
BseLI CCNNNNNNNGG 1 cut(s) 1174
BseMI GCAATG 4 cut(s) 559, 820, 924, 1036
BseMII CTCAG 4 cut(s) 95, 218, 414, 448
BseNI ACTGG 4 cut(s) 325, 504, 1012, 1160
BseRI GAGGAG 2 cut(s) 958, 1066
BseSI GKGCMC 1 cut(s) 1167
BseXI GCAGC 5 cut(s) 186, 301, 465, 545, 561
BshFI GGCC 3 cut(s) 232, 1142, 1202
BsiHKAI GWGCWC 1 cut(s) 1167
BsiSI CCGG 1 cut(s) 738
BslI CCNNNNNNNGG 1 cut(s) 1174
BsmAI GTCTC 3 cut(s) 501, 619, 651
BsmI GAATGC 1 cut(s) 932
BsnI GGCC 3 cut(s) 232, 1142, 1202
Bso31I GGTCTC 1 cut(s) 619
Bsp1286I GDGCHC 2 cut(s) 848, 1167
BspACI CCGC 6 cut(s) 301, 722, 1060, 1121, 1175, 1181
BspANI GGCC 3 cut(s) 232, 1142, 1202
BspCNI CTCAG 4 cut(s) 94, 219, 415, 447
BspMAI CTGCAG 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 619
BsrDI GCAATG 4 cut(s) 559, 820, 924, 1036
BsrI ACTGG 4 cut(s) 325, 504, 1012, 1160
BssECI CCNNGG 1 cut(s) 277
BssNI GRCGYC 2 cut(s) 318, 339
BssT1I CCWWGG 1 cut(s) 277
Bst4CI ACNGT 2 cut(s) 271, 297
BstACI GRCGYC 2 cut(s) 318, 339
BstAPI GCANNNNNTGC 1 cut(s) 558
BstC8I GCNNGC 3 cut(s) 316, 915, 1058
BstDEI CTNAG 4 cut(s) 81, 227, 423, 434
BstF5I GGATG 2 cut(s) 15, 871
BstH2I RGCGCY 1 cut(s) 1014
BstHHI GCGC 1 cut(s) 1013
BstMAI GTCTC 3 cut(s) 501, 619, 651
BstMWI GCNNNNNNNGC 7 cut(s) 289, 555, 558, 719, 760, 1041, 1181
BstNSI RCATGY 1 cut(s) 1060
BstSCI CCNGG 1 cut(s) 737
BstSFI CTRYAG 2 cut(s) 287, 517
BstSLI GKGCMC 1 cut(s) 1167
BstV1I GCAGC 5 cut(s) 186, 301, 465, 545, 561
BstV2I GAAGAC 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 610
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 3 cut(s) 232, 1142, 1202
BtsCI GGATG 2 cut(s) 15, 871
BtsIMutI CAGTG 1 cut(s) 1167
Cac8I GCNNGC 3 cut(s) 316, 915, 1058
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 1 cut(s) 1013
Cfr13I GGNCC 1 cut(s) 1140
CseI GACGC 2 cut(s) 307, 347
Csp6I GTAC 3 cut(s) 887, 1005, 1020
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 6 cut(s) 308, 458, 469, 653, 661, 1057
CviQI GTAC 3 cut(s) 887, 1005, 1020
DdeI CTNAG 4 cut(s) 81, 227, 423, 434
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 848
Eco31I GGTCTC 1 cut(s) 619
Eco32I GATATC 1 cut(s) 1077
Eco81I CCTNAGG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 1140
EcoRI GAATTC 1 cut(s) 950
EcoRV GATATC 1 cut(s) 1077
EcoT14I CCWWGG 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 848
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 6 cut(s) 311, 461, 472, 656, 664, 1060
FatI CATG 6 cut(s) 307, 457, 468, 652, 660, 1056
FauI CCCGC 1 cut(s) 1053
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 7 cut(s) 200, 290, 454, 550, 559, 1122, 1182
FokI GGATG 2 cut(s) 22, 878
FriOI GRGCYC 1 cut(s) 848
Fsp4HI GCNGC 7 cut(s) 200, 290, 454, 550, 559, 1122, 1182
FspBI CTAG 2 cut(s) 1002, 1014
GlaI GCGC 1 cut(s) 1012
GluI GCNGC 7 cut(s) 200, 290, 454, 550, 559, 1122, 1182
GsuI CTGGAG 3 cut(s) 219, 487, 586
HaeII RGCGCY 1 cut(s) 1014
HaeIII GGCC 3 cut(s) 232, 1142, 1202
HapII CCGG 1 cut(s) 738
HgaI GACGC 2 cut(s) 307, 347
HhaI GCGC 1 cut(s) 1013
Hin1I GRCGYC 2 cut(s) 318, 339
Hin1II CATG 6 cut(s) 311, 461, 472, 656, 664, 1060
Hin6I GCGC 1 cut(s) 1011
HinP1I GCGC 1 cut(s) 1011
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 8 cut(s) 219, 245, 584, 630, 734, 821, 938, 1127
HpaII CCGG 1 cut(s) 738
HphI GGTGA 3 cut(s) 555, 736, 806
Hpy166II GTNNAC 2 cut(s) 322, 1165
Hpy188I TCNGA 6 cut(s) 250, 437, 619, 776, 925, 1240
Hpy188III TCNNGA 5 cut(s) 226, 581, 603, 1072, 1134
Hpy8I GTNNAC 2 cut(s) 322, 1165
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 5 cut(s) 340, 472, 1096, 1131, 1192
HpyCH4III ACNGT 2 cut(s) 271, 297
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 5 cut(s) 289, 314, 453, 477, 1165
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 555, 558, 719, 760, 1041, 1181
HpyF3I CTNAG 4 cut(s) 81, 227, 423, 434
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 318, 339
Hsp92II CATG 6 cut(s) 311, 461, 472, 656, 664, 1060
HspAI GCGC 1 cut(s) 1011
LmnI GCTCC 7 cut(s) 24, 207, 447, 537, 605, 1053, 1193
Lsp1109I GCAGC 5 cut(s) 186, 301, 465, 545, 561
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 1002, 1014
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 4 cut(s) 265, 593, 806, 1131
MboII GAAGA 4 cut(s) 39, 343, 1093, 1111
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 2 cut(s) 848, 1167
MluCI AATT 8 cut(s) 51, 665, 693, 878, 950, 975, 1233, 1248
MlyI GAGTC 2 cut(s) 624, 947
MmeI TCCRAC 1 cut(s) 903
MslI CAYNNNNRTG 2 cut(s) 722, 1170
MspI CCGG 1 cut(s) 738
MspR9I CCNGG 1 cut(s) 739
MunI CAATTG 1 cut(s) 975
Mva1269I GAATGC 1 cut(s) 932
MwoI GCNNNNNNNGC 7 cut(s) 289, 555, 558, 719, 760, 1041, 1181
NciI CCSGG 1 cut(s) 739
NlaIII CATG 6 cut(s) 311, 461, 472, 656, 664, 1060
NmuCI GTSAC 4 cut(s) 265, 593, 806, 1131
NspI RCATGY 1 cut(s) 1060
OliI CACNNNNGTG 2 cut(s) 722, 1170
PaeI GCATGC 1 cut(s) 1060
PceI AGGCCT 1 cut(s) 232
PctI GAATGC 1 cut(s) 932
PfeI GAWTC 6 cut(s) 219, 245, 584, 734, 821, 1127
PfoI TCCNGGA 1 cut(s) 737
PkrI GCNGC 7 cut(s) 201, 291, 455, 551, 560, 1123, 1183
PleI GAGTC 2 cut(s) 624, 946
PpsI GAGTC 2 cut(s) 624, 946
PspPI GGNCC 1 cut(s) 1140
PstI CTGCAG 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 295
RsaI GTAC 3 cut(s) 888, 1006, 1021
RsaNI GTAC 3 cut(s) 887, 1005, 1020
RseI CAYNNNNRTG 2 cut(s) 722, 1170
SalI GTCGAC 1 cut(s) 320
SatI GCNGC 7 cut(s) 200, 290, 454, 550, 559, 1122, 1182
Sau96I GGNCC 1 cut(s) 1140
ScaI AGTACT 1 cut(s) 1006
SchI GAGTC 2 cut(s) 624, 947
ScrFI CCNGG 1 cut(s) 739
SduI GDGCHC 2 cut(s) 848, 1167
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 2 cut(s) 287, 517
SmiMI CAYNNNNRTG 2 cut(s) 722, 1170
SmlI CTYRAG 1 cut(s) 993
SmoI CTYRAG 1 cut(s) 993
SpeI ACTAGT 1 cut(s) 1001
SphI GCATGC 1 cut(s) 1060
Sse9I AATT 8 cut(s) 51, 665, 693, 878, 950, 975, 1233, 1248
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 6 cut(s) 301, 722, 1060, 1121, 1175, 1181
SspMI CTAG 2 cut(s) 1002, 1014
StuI AGGCCT 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 737
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 2 cut(s) 271, 297
TaiI ACGT 1 cut(s) 62
TaqI TCGA 6 cut(s) 321, 902, 941, 969, 1073, 1079
TasI AATT 8 cut(s) 51, 665, 693, 878, 950, 975, 1233, 1248
TatI WGTACW 2 cut(s) 1004, 1019
TauI GCSGC 2 cut(s) 1124, 1184
TfiI GAWTC 6 cut(s) 219, 245, 584, 734, 821, 1127
TscAI CASTG 1 cut(s) 1167
TseFI GTSAC 4 cut(s) 265, 593, 806, 1131
TseI GCWGC 5 cut(s) 199, 289, 453, 549, 558
Tsp45I GTSAC 4 cut(s) 265, 593, 806, 1131
TspDTI ATGAA 5 cut(s) 322, 485, 641, 891, 920
TspRI CASTG 1 cut(s) 1167
VneI GTGCAC 1 cut(s) 1163
XapI RAATTY 1 cut(s) 950
XceI RCATGY 1 cut(s) 1060
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 2 cut(s) 1002, 1014
ZrmI AGTACT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.