pycom16g23190

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012281
Physical Location & Seq
Forward (+)
23838595 .. 23840245
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g23190.1

Sequence Viewer

Length: 1521 bp
ATGCAGGTTTGGTTTGTAGTCAACCAGAACAAGTTAAAGAAGAAGATTTGCAGGTTGGTGGATTTATTGGCTTTTCAAATCTCACCTGTGTCACCATTTATTTCCATCTTTCACATTCAATATTTCCTCTCTCCTCTCTCTCTCTCTCTCTCTCAATATTTCAATTTGCAACACATGACACCAAGCCTATACAACTTTTTTGGATGTTTTGGACTCGGACTTGTTTCCACTCAAAGCTGCAATCTTGGGGAGCTCATTGTTCCAAACCCAACCTTTTCTCCTTGGAAAAAACAAAAACAAAAAATGGTGTGGATGTGGAAGCTCCTCGAAATCAAAGAAAATGGGGTTTCTTCAATTCCCACATTCCCACCATACTTTGTTCCTCAGCTTCCTCCCCCACCCCCAAATCTCCAATCAAATAGTTTGAGTTTTGGGAACAAAGTCAGTCCAAGCATACTGCTAATAATCATAATAGTAGCCACTGTTTTCTTTGTTTCTGTGCTGCTCCACCTCCTTGTTAGATTCCTCCTGAGGCCTCCAGCCAGAGATTCAGAGGACTTGGAGAGTGTGACAGTTCTCCAAGGGCAACTGCAGCAACTGTTCCGCCTTCATGATGCAGGCGTCGACCAGTCCTTCATAGACGGCCTCCCTTTCTTCCACTACAAAGCCATCATTGGATTGAAAAACCCTTTTGATTGTGCTGTCTGTTTGTGTGAGTTTGAGGCTGAGGATAACCTTAGATTGCTCCCCAAATGCAGCCATGCCTTCCACATGAAGTGCATTGACACTTGGCTCTTGTCTCACTCCACTTGCCCTCTCTGTAGAGCTACTTTGCTCCCTGATTTCTCGCGCAGCAATGGCTGCTCACCCATTGTTCTTGTTCTTGAATCTGGGAGTGACAGCTCCAGAGAGATGGTCTCTGATAGAGATGGACTCACTTCCAATCTGGGTTTTCATGGAGATATTGAATTGGGGTTGTCATACAAATCTTGCGAAACTGTGGCGAAAAATGAGGCTAACACGGCGGTGATGGTGGATTCTGGGAAAAAGGTAGTGCCTGTGAAGCTTGGGAAGTTCAGAAATGTGGATAGTGGGGGTGAGGGGAGTAGTGACAGCAATGTGGATTCAAGGAGGTGCTTTTCTATGGGCTCATTTGCGTATGTGATGGATGACAAATCTGAATTGCGAGTACCGATTAGAGCCTCGATGAAAAGGCAAGCAATGTCGGAATGCGATTGTGAGTCGAAGAGAGAATTCAAGTTTAGTGGGGTTGAAGCAATTGGAAGCCTTGAAACTCAAGGAACTAGAACTGGTGCTAGTAGTACTAATGGCAGTGGCAGTAATGGCATTGGGAGGAGCATGCGGGAGAGCTTCTCGATATCGAAGATTTGGCTTATGGGGAAGAAGGAGAAGCCAAATGGGGCGGCAGATTCGTCACGAAGGGCCTTTTCATTTCGCTTTCCAGTGCACCAGAGTGCGGTGGCGGCAGAGGAGGGAGCGAAGGCAGAATGTTGGGAGTGA

Protein Analysis

507

Amino Acids

56.13

Weight (kDa)

8.39

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 228 - 275 9.2e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 230 - 277 1.4e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 232 - 275 5e-14 Ring finger domain
zf-RING_11 PF17123 232 - 261 4e-10 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18650 AT4G30400
fragaria_vesca FvH4_4g09580 FvH4_4g09580
malus_domestica MD13G1260900.v1.1 MD16G1263000.v1.1
prunus_persica Prupe.1G097100_v2.0.a1
pyrus_communis pycom16g23190
rosa_chinensis RchiOBHm_Chr4g0404881
rosa_laevigata RLG00000008897
rosa_multiflora Rmu_sc0001179.1_g000015
rosa_roxburghii Rroxscaffold_5G00349010
rosa_rugosa Rorug04G0049700 Rorug04G0049700
rosa_samantha Rh4AG125700 Rh4BG119500 Rh4CG133900 Rh4DG119300
rosa_wichuraiana Rw4G010170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 624
AccII CGCG 1 cut(s) 850
AciI CCGC 6 cut(s) 604, 1025, 1363, 1424, 1478, 1484
AcsI RAATTY 1 cut(s) 1253
AcyI GRCGYC 1 cut(s) 621
AfaI GTAC 2 cut(s) 1191, 1324
AfiI CCNNNNNNNGG 1 cut(s) 1477
AleI CACNNNNGTG 2 cut(s) 1025, 1473
AluBI AGCT 8 cut(s) 237, 253, 322, 388, 827, 903, 1066, 1371
AluI AGCT 8 cut(s) 237, 253, 322, 388, 827, 903, 1066, 1371
Alw21I GWGCWC 2 cut(s) 255, 1470
Alw26I GTCTC 2 cut(s) 804, 922
Alw44I GTGCAC 1 cut(s) 1466
AlwNI CAGNNNCTG 1 cut(s) 598
AoxI GGCC 3 cut(s) 533, 643, 1443
ApaLI GTGCAC 1 cut(s) 1466
ApeKI GCWGC 6 cut(s) 237, 502, 592, 756, 852, 861
ApoI RAATTY 1 cut(s) 1253
AspLEI GCGC 1 cut(s) 852
AspS9I GGNCC 1 cut(s) 1443
AsuHPI GGTGA 5 cut(s) 75, 84, 858, 1039, 1109
AxyI CCTNAGG 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 1470
BanII GRGCYC 2 cut(s) 255, 1151
Bbv12I GWGCWC 2 cut(s) 255, 1470
BbvCI CCTCAGC 2 cut(s) 384, 726
BbvI GCAGC 6 cut(s) 224, 489, 604, 768, 848, 864
BccI CCATC 6 cut(s) 113, 677, 907, 923, 1024, 1159
BceAI ACGGC 2 cut(s) 658, 1038
BcoDI GTCTC 2 cut(s) 804, 922
BfaI CTAG 2 cut(s) 1305, 1317
BfmI CTRYAG 2 cut(s) 590, 820
BfuAI ACCTGC 1 cut(s) 42
BisI GCNGC 8 cut(s) 238, 503, 593, 757, 853, 862, 1425, 1485
BlsI GCNGC 8 cut(s) 239, 504, 594, 758, 854, 863, 1426, 1486
BmcAI AGTACT 1 cut(s) 1324
BmgT120I GGNCC 1 cut(s) 1443
BmsI GCATC 1 cut(s) 604
BplI GAGNNNNNCTC 6 cut(s) 902, 934, 918, 950, 1358, 1390
BpmI CTGGAG 2 cut(s) 522, 889
Bpu10I CCTNAGC 2 cut(s) 384, 726
BpuEI CTTGAG 1 cut(s) 1281
BsaHI GRCGYC 1 cut(s) 621
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 2 cut(s) 281, 580
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 1 cut(s) 1477
Bse1I ACTGG 3 cut(s) 628, 1315, 1463
Bse21I CCTNAGG 1 cut(s) 530
Bse3DI GCAATG 3 cut(s) 862, 1123, 1227
BseDI CCNNGG 2 cut(s) 281, 580
BseGI GGATG 3 cut(s) 209, 318, 1174
BseLI CCNNNNNNNGG 1 cut(s) 1477
BseMI GCAATG 3 cut(s) 862, 1123, 1227
BseMII CTCAG 3 cut(s) 398, 521, 717
BseNI ACTGG 3 cut(s) 628, 1315, 1463
BseRI GAGGAG 4 cut(s) 123, 314, 1369, 1505
BseSI GKGCMC 1 cut(s) 1470
BseXI GCAGC 6 cut(s) 224, 489, 604, 768, 848, 864
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 3 cut(s) 535, 645, 1445
BsiHKAI GWGCWC 2 cut(s) 255, 1470
BslI CCNNNNNNNGG 1 cut(s) 1477
BsmAI GTCTC 2 cut(s) 804, 922
BsmI GAATGC 1 cut(s) 1235
BsnI GGCC 3 cut(s) 535, 645, 1445
Bso31I GGTCTC 1 cut(s) 922
Bsp1286I GDGCHC 3 cut(s) 255, 1151, 1470
BspACI CCGC 6 cut(s) 604, 1025, 1363, 1424, 1478, 1484
BspANI GGCC 3 cut(s) 535, 645, 1445
BspCNI CTCAG 3 cut(s) 397, 522, 718
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 1 cut(s) 610
BspMAI CTGCAG 1 cut(s) 594
BspMI ACCTGC 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 922
BsrDI GCAATG 3 cut(s) 862, 1123, 1227
BsrI ACTGG 3 cut(s) 628, 1315, 1463
BssECI CCNNGG 2 cut(s) 281, 580
BssNI GRCGYC 1 cut(s) 621
BssT1I CCWWGG 2 cut(s) 281, 580
Bst4CI ACNGT 4 cut(s) 484, 574, 600, 1000
Bst6I CTCTTC 1 cut(s) 1241
BstACI GRCGYC 1 cut(s) 621
BstAPI GCANNNNNTGC 1 cut(s) 861
BstC8I GCNNGC 3 cut(s) 619, 1218, 1361
BstDEI CTNAG 4 cut(s) 384, 530, 726, 737
BstF5I GGATG 3 cut(s) 209, 318, 1174
BstFNI CGCG 1 cut(s) 850
BstHHI GCGC 1 cut(s) 852
BstMAI GTCTC 2 cut(s) 804, 922
BstMWI GCNNNNNNNGC 7 cut(s) 592, 858, 861, 1022, 1063, 1344, 1484
BstNSI RCATGY 1 cut(s) 1363
BstSFI CTRYAG 2 cut(s) 590, 820
BstSLI GKGCMC 1 cut(s) 1470
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 6 cut(s) 224, 489, 604, 768, 848, 864
BstXI CCANNNNNNTGG 1 cut(s) 913
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 3 cut(s) 535, 645, 1445
BtsCI GGATG 3 cut(s) 209, 318, 1174
BtsI GCAGTG 1 cut(s) 1339
BtsIMutI CAGTG 3 cut(s) 480, 1339, 1470
BveI ACCTGC 1 cut(s) 42
Cac8I GCNNGC 3 cut(s) 619, 1218, 1361
CaiI CAGNNNCTG 1 cut(s) 598
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 852
Cfr13I GGNCC 1 cut(s) 1443
CseI GACGC 1 cut(s) 610
Csp6I GTAC 2 cut(s) 1190, 1323
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviAII CATG 6 cut(s) 175, 611, 761, 772, 956, 1360
CviQI GTAC 2 cut(s) 1190, 1323
DdeI CTNAG 4 cut(s) 384, 530, 726, 737
Eam1104I CTCTTC 1 cut(s) 1241
EarI CTCTTC 1 cut(s) 1241
EciI GGCGGA 1 cut(s) 593
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 281, 580
Eco147I AGGCCT 1 cut(s) 535
Eco24I GRGCYC 2 cut(s) 255, 1151
Eco31I GGTCTC 1 cut(s) 922
Eco32I GATATC 1 cut(s) 1380
Eco53kI GAGCTC 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 1 cut(s) 1253
EcoRV GATATC 1 cut(s) 1380
EcoT14I CCWWGG 2 cut(s) 281, 580
EcoT38I GRGCYC 2 cut(s) 255, 1151
ErhI CCWWGG 2 cut(s) 281, 580
FaeI CATG 6 cut(s) 178, 614, 764, 775, 959, 1363
FatI CATG 6 cut(s) 174, 610, 760, 771, 955, 1359
FauI CCCGC 1 cut(s) 1356
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 8 cut(s) 238, 503, 593, 757, 853, 862, 1425, 1485
FokI GGATG 3 cut(s) 216, 325, 1181
FriOI GRGCYC 2 cut(s) 255, 1151
Fsp4HI GCNGC 8 cut(s) 238, 503, 593, 757, 853, 862, 1425, 1485
FspBI CTAG 2 cut(s) 1305, 1317
GlaI GCGC 1 cut(s) 851
GluI GCNGC 8 cut(s) 238, 503, 593, 757, 853, 862, 1425, 1485
GsuI CTGGAG 2 cut(s) 522, 889
HaeIII GGCC 3 cut(s) 535, 645, 1445
HgaI GACGC 1 cut(s) 610
HhaI GCGC 1 cut(s) 852
Hin1I GRCGYC 1 cut(s) 621
Hin1II CATG 6 cut(s) 178, 614, 764, 775, 959, 1363
Hin6I GCGC 1 cut(s) 850
HinP1I GCGC 1 cut(s) 850
HincII GTYRAC 2 cut(s) 22, 625
HindII GTYRAC 2 cut(s) 22, 625
HindIII AAGCTT 1 cut(s) 1064
HinfI GANTC 9 cut(s) 213, 522, 548, 887, 933, 1037, 1124, 1241, 1430
HphI GGTGA 5 cut(s) 75, 84, 858, 1039, 1109
Hpy166II GTNNAC 3 cut(s) 22, 625, 1468
Hpy188I TCNGA 6 cut(s) 218, 553, 922, 1079, 1180, 1228
Hpy188III TCNNGA 6 cut(s) 529, 611, 884, 906, 1375, 1437
Hpy8I GTNNAC 3 cut(s) 22, 625, 1468
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 6 cut(s) 617, 643, 775, 1399, 1434, 1495
HpyCH4III ACNGT 4 cut(s) 484, 574, 600, 1000
HpyCH4V TGCA 9 cut(s) 4, 51, 169, 240, 592, 617, 756, 780, 1468
HpyF10VI GCNNNNNNNGC 7 cut(s) 592, 858, 861, 1022, 1063, 1344, 1484
HpyF3I CTNAG 4 cut(s) 384, 530, 726, 737
Hsp92I GRCGYC 1 cut(s) 621
Hsp92II CATG 6 cut(s) 178, 614, 764, 775, 959, 1363
HspAI GCGC 1 cut(s) 850
LmnI GCTCC 8 cut(s) 250, 327, 510, 750, 840, 908, 1356, 1496
Lsp1109I GCAGC 6 cut(s) 224, 489, 604, 768, 848, 864
LweI GCATC 1 cut(s) 604
MaeI CTAG 2 cut(s) 1305, 1317
MaeIII GTNAC 5 cut(s) 90, 568, 896, 1109, 1434
MboII GAAGA 7 cut(s) 52, 55, 342, 646, 1258, 1396, 1414
MfeI CAATTG 1 cut(s) 1278
MhlI GDGCHC 3 cut(s) 255, 1151, 1470
MluCI AATT 6 cut(s) 163, 354, 968, 1181, 1253, 1278
MlyI GAGTC 3 cut(s) 207, 927, 1250
MmeI TCCRAC 1 cut(s) 1206
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 2 cut(s) 1025, 1473
MunI CAATTG 1 cut(s) 1278
Mva1269I GAATGC 1 cut(s) 1235
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 7 cut(s) 592, 858, 861, 1022, 1063, 1344, 1484
NlaIII CATG 6 cut(s) 178, 614, 764, 775, 959, 1363
NmuCI GTSAC 5 cut(s) 90, 568, 896, 1109, 1434
NspI RCATGY 1 cut(s) 1363
OliI CACNNNNGTG 2 cut(s) 1025, 1473
PaeI GCATGC 1 cut(s) 1363
PagI TCATGA 1 cut(s) 610
PceI AGGCCT 1 cut(s) 535
PctI GAATGC 1 cut(s) 1235
PfeI GAWTC 6 cut(s) 522, 548, 887, 1037, 1124, 1430
PkrI GCNGC 8 cut(s) 239, 504, 594, 758, 854, 863, 1426, 1486
PleI GAGTC 3 cut(s) 207, 927, 1249
PpsI GAGTC 3 cut(s) 207, 927, 1249
Psp124BI GAGCTC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 1443
PstI CTGCAG 1 cut(s) 594
PstNI CAGNNNCTG 1 cut(s) 598
RsaI GTAC 2 cut(s) 1191, 1324
RsaNI GTAC 2 cut(s) 1190, 1323
RseI CAYNNNNRTG 2 cut(s) 1025, 1473
SacI GAGCTC 1 cut(s) 255
SalI GTCGAC 1 cut(s) 623
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 8 cut(s) 238, 503, 593, 757, 853, 862, 1425, 1485
Sau96I GGNCC 1 cut(s) 1443
ScaI AGTACT 1 cut(s) 1324
SchI GAGTC 3 cut(s) 207, 927, 1250
SduI GDGCHC 3 cut(s) 255, 1151, 1470
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 2 cut(s) 590, 820
SmiMI CAYNNNNRTG 2 cut(s) 1025, 1473
SmlI CTYRAG 1 cut(s) 1296
SmoI CTYRAG 1 cut(s) 1296
SphI GCATGC 1 cut(s) 1363
Sse9I AATT 6 cut(s) 163, 354, 968, 1181, 1253, 1278
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 6 cut(s) 604, 1025, 1363, 1424, 1478, 1484
SspI AATATT 2 cut(s) 122, 158
SspMI CTAG 2 cut(s) 1305, 1317
SstI GAGCTC 1 cut(s) 255
StuI AGGCCT 1 cut(s) 535
StyI CCWWGG 2 cut(s) 281, 580
TaaI ACNGT 4 cut(s) 484, 574, 600, 1000
TaqI TCGA 6 cut(s) 327, 624, 1205, 1244, 1376, 1382
TasI AATT 6 cut(s) 163, 354, 968, 1181, 1253, 1278
TatI WGTACW 1 cut(s) 1322
TauI GCSGC 2 cut(s) 1427, 1487
TfiI GAWTC 6 cut(s) 522, 548, 887, 1037, 1124, 1430
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 3 cut(s) 487, 1339, 1470
TseFI GTSAC 5 cut(s) 90, 568, 896, 1109, 1434
TseI GCWGC 6 cut(s) 237, 502, 592, 756, 852, 861
Tsp45I GTSAC 5 cut(s) 90, 568, 896, 1109, 1434
TspDTI ATGAA 6 cut(s) 599, 625, 788, 944, 1223, 1440
TspRI CASTG 3 cut(s) 487, 1339, 1470
VneI GTGCAC 1 cut(s) 1466
XapI RAATTY 1 cut(s) 1253
XceI RCATGY 1 cut(s) 1363
XmiI GTMKAC 1 cut(s) 624
XspI CTAG 2 cut(s) 1305, 1317
ZrmI AGTACT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.