MD16G1274300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015976
Physical Location & Seq
Forward (+)
36672371 .. 36672733
363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1274300.v1.1.491

Sequence Viewer

Length: 363 bp
ATGAGCCTTAATTGCCTAACATGCCAGGTCCTTCAGAGGACAAGTTCCTCCGGAGATAGAGATCATGCGAGCAAGGAGAAGGCTTATAAGAAACTTTGTTGTGTAAACATCAATATCGACAGAAGCTGGTCCGGGAACTTAACTTCGCCACCTTATGAGCAAATTGAAAACTCGTCTATCTTCATGCCGGAGAAGAAGGTGGTGAAGAAGGGGCCTGGTCATCGCCGGCTTATGACTACAGGTGGAGTTGCATATGAAGGGAGCGTTGAACCGCGCTTGGTCAGGAGCTCCGGAATGAGAAGGGATTGGAGCTTTGAGAATTTGTCTCTCCTCAGAAATGAGAAGAAAGGGAGAAACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.57

Weight (kDa)

9.73

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35215 AT5G46770
fragaria_vesca FvH4_5g22090
malus_domestica MD16G1274300.v1.1
prunus_persica Prupe.5G036400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0209741
rosa_laevigata RLG00000003097
rosa_multiflora Rmu_sc0002616.1_g000018
rosa_roxburghii Rroxscaffold_3G00249110
rosa_rugosa Rorug07G0116000
rosa_samantha Rh7AG249500 Rh7BG243400 Rh7CG265500 Rh7DG255300
rosa_wichuraiana Rw7G021150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
AccII CGCG 1 cut(s) 274
AccIII TCCGGA 2 cut(s) 50, 290
AciI CCGC 1 cut(s) 272
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 17
AgsI TTSAA 2 cut(s) 167, 269
AjnI CCWGG 2 cut(s) 24, 214
AluBI AGCT 3 cut(s) 126, 288, 312
AluI AGCT 3 cut(s) 126, 288, 312
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 126
Aor13HI TCCGGA 2 cut(s) 50, 290
AoxI GGCC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 3 cut(s) 28, 129, 212
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 214
AvaII GGWCC 2 cut(s) 28, 129
BanII GRGCYC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 290
BciT130I CCWGG 2 cut(s) 26, 216
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 237
Bme1390I CCNGG 3 cut(s) 26, 133, 216
Bme18I GGWCC 2 cut(s) 28, 129
BmgT120I GGNCC 3 cut(s) 28, 129, 212
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 3 cut(s) 26, 133, 216
BpuMI CCSGG 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 60
BsaWI WCCGGW 2 cut(s) 50, 290
Bse118I RCCGGY 1 cut(s) 225
Bse8I GATNNNNATC 1 cut(s) 60
BseAI TCCGGA 2 cut(s) 50, 290
BseBI CCWGG 2 cut(s) 26, 216
BseJI GATNNNNATC 1 cut(s) 60
BseMII CTCAG 1 cut(s) 346
BseRI GAGGAG 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 274
BshFI GGCC 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 5 cut(s) 51, 132, 188, 226, 291
BsmAI GTCTC 1 cut(s) 330
BsnI GGCC 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 290
Bsp13I TCCGGA 2 cut(s) 50, 290
Bsp143I GATC 1 cut(s) 61
BspACI CCGC 1 cut(s) 272
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 1 cut(s) 345
BspEI TCCGGA 2 cut(s) 50, 290
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 225
BssAI RCCGGY 1 cut(s) 225
BssMI GATC 1 cut(s) 61
Bst2UI CCWGG 2 cut(s) 26, 216
BstC8I GCNNGC 2 cut(s) 70, 227
BstDEI CTNAG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 274
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 1 cut(s) 64
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 12, 21
BstNI CCWGG 2 cut(s) 26, 216
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 3 cut(s) 24, 131, 214
BstSFI CTRYAG 1 cut(s) 237
BstUI CGCG 1 cut(s) 274
BsuRI GGCC 1 cut(s) 214
BtgZI GCGATG 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 70, 227
CaiI CAGNNNCTG 1 cut(s) 126
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 3 cut(s) 28, 129, 212
CviAII CATG 3 cut(s) 21, 65, 184
CviJI RGCY 7 cut(s) 6, 83, 126, 214, 229, 288, 312
CviKI_1 RGCY 7 cut(s) 6, 83, 126, 214, 229, 288, 312
DdeI CTNAG 1 cut(s) 332
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
Ecl136II GAGCTC 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 290
Eco47I GGWCC 2 cut(s) 28, 129
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 288
EcoO109I RGGNCCY 2 cut(s) 28, 212
EcoRII CCWGG 2 cut(s) 24, 214
EcoT38I GRGCYC 1 cut(s) 290
FaeI CATG 3 cut(s) 24, 68, 187
FaiI YATR 9 cut(s) 22, 66, 87, 156, 185, 233, 253, 255, 361
FatI CATG 3 cut(s) 20, 64, 183
FauNDI CATATG 1 cut(s) 253
FriOI GRGCYC 1 cut(s) 290
GlaI GCGC 1 cut(s) 275
HaeIII GGCC 1 cut(s) 214
HapII CCGG 5 cut(s) 51, 132, 188, 226, 291
HhaI GCGC 1 cut(s) 276
Hin1II CATG 3 cut(s) 24, 68, 187
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HpaII CCGG 5 cut(s) 51, 132, 188, 226, 291
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 36, 335
Hpy188III TCNNGA 3 cut(s) 51, 283, 291
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 6 cut(s) 41, 73, 190, 202, 251, 294
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 12, 21
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 3 cut(s) 24, 68, 187
HspAI GCGC 1 cut(s) 274
Kpn2I TCCGGA 2 cut(s) 50, 290
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 4 cut(s) 261, 285, 293, 309
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 4 cut(s) 172, 205, 217, 355
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 3 cut(s) 10, 162, 319
MnlI CCTC 3 cut(s) 30, 58, 341
MroI TCCGGA 2 cut(s) 50, 290
MroNI GCCGGC 1 cut(s) 225
MseI TTAA 2 cut(s) 9, 140
MspI CCGG 5 cut(s) 51, 132, 188, 226, 291
MspR9I CCNGG 3 cut(s) 26, 133, 216
MvaI CCWGG 2 cut(s) 26, 216
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 2 cut(s) 12, 21
NaeI GCCGGC 1 cut(s) 227
NciI CCSGG 1 cut(s) 133
NdeI CATATG 1 cut(s) 253
NdeII GATC 1 cut(s) 61
NgoMIV GCCGGC 1 cut(s) 225
NlaIII CATG 3 cut(s) 24, 68, 187
NlaIV GGNNCC 1 cut(s) 213
NspI RCATGY 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 227
PfoI TCCNGGA 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 28
PsiI TTATAA 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 2 cut(s) 24, 214
PspGI CCWGG 2 cut(s) 24, 214
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 3 cut(s) 28, 129, 212
PspPPI RGGWCCY 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 126
SacI GAGCTC 1 cut(s) 290
SaqAI TTAA 2 cut(s) 9, 140
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 3 cut(s) 28, 129, 212
ScrFI CCNGG 3 cut(s) 26, 133, 216
SduI GDGCHC 1 cut(s) 290
SetI ASST 7 cut(s) 30, 128, 154, 201, 244, 290, 314
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 2 cut(s) 28, 129
Sse9I AATT 3 cut(s) 10, 162, 319
SsiI CCGC 1 cut(s) 272
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 24, 131, 214
TaqI TCGA 1 cut(s) 117
TasI AATT 3 cut(s) 10, 162, 319
Tru1I TTAA 2 cut(s) 9, 140
Tru9I TTAA 2 cut(s) 9, 140
TspDTI ATGAA 2 cut(s) 172, 270
VpaK11BI GGWCC 2 cut(s) 28, 129
XapI RAATTY 1 cut(s) 319
XceI RCATGY 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.