Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0014953

Physical Location & Seq

Genomic Coordinates Reverse (-)

91553 .. 93431

Gene Structure 1879 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1001500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 672 bp

ATGGAGAAGTATCAAATGTTCTTTCCTTGCACCTCATCAGCTAATTACCCAAACCCTAATTCCACAAATATAGATCATCATCACCGGGTGGGAAGCTCTCACGTTTACACGAATAACTTTGATGGGCGTGTTGATCAAACTTCAGATGCATTTTTGGGGTTGAAATCGACGATGACGACAACAGAAAACCACGTTGGATCATCGTCAGATGAAGTATTAGTTCATAAAGATGGTCATCCGTACCAACAATATTATCAGCAGCATTATCCGAGTGCAAATGCAGGCATGAGTAATGTTATTGTTGGAGCTGATCACAGCAGCGTCGATCCTCATCGGGCCAATAATAATGATTCAGGGAAAAAGAAAAAGGGGGAGAAGAAGATGAAAAAGCCGAAATATGCTTTTCAAACAAGAAGTCAGGTCGATATACTTGACGATGGCTACCGGTGGAGAAAATACGGCCAAAAAGCTGTGAAAGCCAACAAGTTTCCCAGAAGCTACTACCGATGCACGTATGAAGGGTGCAATGTTAAAAAGCAAGTCCAACGCCTAAGCAAGGACGAAGGCGTTGTGGTGACCACTTACGAAGGAATGCACACCCATCCCATCGACAAGCCTTCCGACAATTTTGAGCGTATCTTGAATCAGATGCAAATTTACACCCCCTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010015 GO:0010053 GO:0010055 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022622 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032101 GO:0032104 GO:0032107 GO:0032501 GO:0032502 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043620 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048583 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0090558 GO:0090627 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

224

Amino Acids

25.6

Weight (kDa)

9.14

Isoelectric Point (pI)

33.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	145 - 202	7.9e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0014953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01970
fragaria_vesca	FvH4_6g53770
malus_domestica	MD09G1008800.v1.1 MD17G1001500.v1.1
prunus_persica	Prupe.3G308200_v2.0.a1
pyrus_communis	pycom111g00710
rosa_chinensis	RchiOBHm_Chr2g0175911
rosa_laevigata	RLG00000022363
rosa_multiflora	Rmu_ssc0000114.1_g000002
rosa_roxburghii	Rroxscaffold_2G00076960
rosa_rugosa	Rorug02G0588400
rosa_samantha	Rh2AG667700 Rh2BG679200 Rh2CG642000 Rh2DG692700
rosa_wichuraiana	Rw2G054730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	2 cut(s)	205, 320
AcoI	YGGCCR	1 cut(s)	460
AcsI	RAATTY	1 cut(s)	654
AcuI	CTGAAG	1 cut(s)	126
AdeI	CACNNNGTG	1 cut(s)	88
AfaI	GTAC	1 cut(s)	242
AfiI	CCNNNNNNNGG	1 cut(s)	556
AgeI	ACCGGT	1 cut(s)	444
AgsI	TTSAA	3 cut(s)	163, 407, 643
AjuI	GAANNNNNNNTTGG	4 cut(s)	43, 75, 177, 209
AluBI	AGCT	5 cut(s)	41, 96, 308, 470, 498
AluI	AGCT	5 cut(s)	41, 96, 308, 470, 498
AlwI	GGATC	2 cut(s)	205, 320
AoxI	GGCC	2 cut(s)	336, 460
ApeKI	GCWGC	2 cut(s)	259, 318
ApoI	RAATTY	1 cut(s)	654
ArsI	GACNNNNNNTTYG	2 cut(s)	400, 432
AsiGI	ACCGGT	1 cut(s)	444
AspS9I	GGNCC	1 cut(s)	336
AsuC2I	CCSGG	1 cut(s)	86
AsuHPI	GGTGA	2 cut(s)	74, 586
BbvI	GCAGC	2 cut(s)	271, 330
BccI	CCATC	5 cut(s)	116, 224, 431, 609, 614
BceAI	ACGGC	1 cut(s)	475
BclI	TGATCA	2 cut(s)	133, 310
BcnI	CCSGG	1 cut(s)	86
BisI	GCNGC	2 cut(s)	260, 319
BlsI	GCNGC	2 cut(s)	261, 320
Bme1390I	CCNGG	1 cut(s)	86
BmgT120I	GGNCC	1 cut(s)	336
BmrFI	CCNGG	1 cut(s)	86
BmsI	GCATC	3 cut(s)	136, 497, 639
Bpu10I	CCTNAGC	1 cut(s)	551
BpuMI	CCSGG	1 cut(s)	86
BsaAI	YACGTR	1 cut(s)	513
BsaBI	GATNNNNATC	3 cut(s)	78, 234, 330
BsaWI	WCCGGW	1 cut(s)	444
Bsc4I	CCNNNNNNNGG	1 cut(s)	556
Bse118I	RCCGGY	1 cut(s)	444
Bse3DI	GCAATG	1 cut(s)	532
Bse8I	GATNNNNATC	3 cut(s)	78, 234, 330
BseGI	GGATG	2 cut(s)	235, 601
BseJI	GATNNNNATC	3 cut(s)	78, 234, 330
BseLI	CCNNNNNNNGG	1 cut(s)	556
BseMI	GCAATG	1 cut(s)	532
BseXI	GCAGC	2 cut(s)	271, 330
BshFI	GGCC	2 cut(s)	338, 462
BshTI	ACCGGT	1 cut(s)	444
BsiSI	CCGG	2 cut(s)	85, 445
BslI	CCNNNNNNNGG	1 cut(s)	556
BsmI	GAATGC	1 cut(s)	597
BsnI	GGCC	2 cut(s)	338, 462
Bsp143I	GATC	5 cut(s)	73, 133, 197, 310, 325
BspANI	GGCC	2 cut(s)	338, 462
BspPI	GGATC	2 cut(s)	205, 320
BsrDI	GCAATG	1 cut(s)	532
BsrFI	RCCGGY	1 cut(s)	444
BssAI	RCCGGY	1 cut(s)	444
BssMI	GATC	5 cut(s)	73, 133, 197, 310, 325
BstBAI	YACGTR	1 cut(s)	513
BstC8I	GCNNGC	1 cut(s)	283
BstDEI	CTNAG	1 cut(s)	551
BstEII	GGTNACC	1 cut(s)	574
BstENI	CCTNNNNNAGG	1 cut(s)	554
BstF5I	GGATG	2 cut(s)	235, 601
BstKTI	GATC	5 cut(s)	76, 136, 200, 313, 328
BstMBI	GATC	5 cut(s)	73, 133, 197, 310, 325
BstMWI	GCNNNNNNNGC	1 cut(s)	476
BstPI	GGTNACC	1 cut(s)	574
BstSCI	CCNGG	1 cut(s)	84
BstV1I	GCAGC	2 cut(s)	271, 330
BsuRI	GGCC	2 cut(s)	338, 462
BtsCI	GGATG	2 cut(s)	235, 601
Cac8I	GCNNGC	1 cut(s)	283
Cfr10I	RCCGGY	1 cut(s)	444
Cfr13I	GGNCC	1 cut(s)	336
CseI	GACGC	1 cut(s)	310
Csp6I	GTAC	1 cut(s)	241
CspAI	ACCGGT	1 cut(s)	444
CviAII	CATG	1 cut(s)	286
CviQI	GTAC	1 cut(s)	241
DdeI	CTNAG	1 cut(s)	551
DpnI	GATC	5 cut(s)	75, 135, 199, 312, 327
DpnII	GATC	5 cut(s)	73, 133, 197, 310, 325
DraIII	CACNNNGTG	1 cut(s)	88
EaeI	YGGCCR	1 cut(s)	460
Eco57I	CTGAAG	1 cut(s)	126
Eco91I	GGTNACC	1 cut(s)	574
EcoNI	CCTNNNNNAGG	1 cut(s)	554
EcoO65I	GGTNACC	1 cut(s)	574
EcoT22I	ATGCAT	1 cut(s)	151
FaeI	CATG	1 cut(s)	289
FaiI	YATR	6 cut(s)	71, 225, 287, 399, 428, 516
FatI	CATG	1 cut(s)	285
FbaI	TGATCA	2 cut(s)	133, 310
Fnu4HI	GCNGC	2 cut(s)	260, 319
FokI	GGATG	2 cut(s)	222, 588
Fsp4HI	GCNGC	2 cut(s)	260, 319
GluI	GCNGC	2 cut(s)	260, 319
HaeIII	GGCC	2 cut(s)	338, 462
HapII	CCGG	2 cut(s)	85, 445
HgaI	GACGC	1 cut(s)	310
Hin1II	CATG	1 cut(s)	289
HinfI	GANTC	2 cut(s)	350, 643
HpaII	CCGG	2 cut(s)	85, 445
HphI	GGTGA	2 cut(s)	74, 586
Hpy166II	GTNNAC	1 cut(s)	106
Hpy188I	TCNGA	6 cut(s)	145, 208, 270, 622, 648, 671
Hpy188III	TCNNGA	1 cut(s)	640
Hpy8I	GTNNAC	1 cut(s)	106
Hpy99I	CGWCG	2 cut(s)	172, 326
HpyAV	CCTTC	4 cut(s)	512, 557, 581, 627
HpyCH4IV	ACGT	3 cut(s)	102, 192, 512
HpyCH4V	TGCA	8 cut(s)	30, 149, 275, 281, 510, 525, 595, 652
HpyF10VI	GCNNNNNNNGC	1 cut(s)	476
HpyF3I	CTNAG	1 cut(s)	551
HpySE526I	ACGT	3 cut(s)	102, 192, 512
Hsp92II	CATG	1 cut(s)	289
Ksp22I	TGATCA	2 cut(s)	133, 310
Kzo9I	GATC	5 cut(s)	73, 133, 197, 310, 325
LmnI	GCTCC	1 cut(s)	305
LpnPI	CCDG	6 cut(s)	98, 267, 339, 404, 458, 505
Lsp1109I	GCAGC	2 cut(s)	271, 330
LweI	GCATC	3 cut(s)	136, 497, 639
MaeII	ACGT	3 cut(s)	102, 192, 512
MaeIII	GTNAC	1 cut(s)	574
MalI	GATC	5 cut(s)	75, 135, 199, 312, 327
MboI	GATC	5 cut(s)	73, 133, 197, 310, 325
MboII	GAAGA	2 cut(s)	388, 391
MluCI	AATT	4 cut(s)	43, 58, 625, 654
MmeI	TCCRAC	4 cut(s)	175, 283, 568, 645
MnlI	CCTC	2 cut(s)	43, 339
Mph1103I	ATGCAT	1 cut(s)	151
MseI	TTAA	1 cut(s)	531
MslI	CAYNNNNRTG	1 cut(s)	228
MspI	CCGG	2 cut(s)	85, 445
MspR9I	CCNGG	1 cut(s)	86
Mva1269I	GAATGC	1 cut(s)	597
MwoI	GCNNNNNNNGC	1 cut(s)	476
NciI	CCSGG	1 cut(s)	86
NdeII	GATC	5 cut(s)	73, 133, 197, 310, 325
NlaIII	CATG	1 cut(s)	289
NmuCI	GTSAC	1 cut(s)	574
NsiI	ATGCAT	1 cut(s)	151
PcsI	WCGNNNNNNNCGW	1 cut(s)	173
PctI	GAATGC	1 cut(s)	597
PfeI	GAWTC	2 cut(s)	350, 643
PinAI	ACCGGT	1 cut(s)	444
PkrI	GCNGC	2 cut(s)	261, 320
Ppu21I	YACGTR	1 cut(s)	513
PspEI	GGTNACC	1 cut(s)	574
PspPI	GGNCC	1 cut(s)	336
PsrI	GAACNNNNNNTAC	1 cut(s)	34
RsaI	GTAC	1 cut(s)	242
RsaNI	GTAC	1 cut(s)	241
RseI	CAYNNNNRTG	1 cut(s)	228
SaqAI	TTAA	1 cut(s)	531
SatI	GCNGC	2 cut(s)	260, 319
Sau3AI	GATC	5 cut(s)	73, 133, 197, 310, 325
Sau96I	GGNCC	1 cut(s)	336
ScrFI	CCNGG	1 cut(s)	86
SfaNI	GCATC	3 cut(s)	136, 497, 639
SmiMI	CAYNNNNRTG	1 cut(s)	228
Sse9I	AATT	4 cut(s)	43, 58, 625, 654
SspI	AATATT	1 cut(s)	251
StyD4I	CCNGG	1 cut(s)	84
TaiI	ACGT	3 cut(s)	105, 195, 515
TaqI	TCGA	4 cut(s)	167, 324, 423, 609
TasI	AATT	4 cut(s)	43, 58, 625, 654
TfiI	GAWTC	2 cut(s)	350, 643
Tru1I	TTAA	1 cut(s)	531
Tru9I	TTAA	1 cut(s)	531
TseFI	GTSAC	1 cut(s)	574
TseI	GCWGC	2 cut(s)	259, 318
Tsp45I	GTSAC	1 cut(s)	574
TspDTI	ATGAA	4 cut(s)	212, 225, 398, 531
TspGWI	ACGGA	1 cut(s)	228
XagI	CCTNNNNNAGG	1 cut(s)	554
XapI	RAATTY	1 cut(s)	654
Zsp2I	ATGCAT	1 cut(s)	151

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.