MD17G1003500.v1.1

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010061
Physical Location & Seq
Forward (+)
320096 .. 323373
3278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1003500.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGCTTCGGCTATTCTTTCTGTGGCCAAACCATCTCTTCAGGCCAATGGAAAGGGATTTGCAGAATTCTCAGGTCTCCGTACCTCATTAGCTTGCCTTCCTTTCGGAAGGAAGACCTCAGACGATTTATTCTCAGTCATTGCCTTCCAGACCTCTGCTTTGGGAAGCAACGGAGTATACAAAAAAGGAGTAACGGAGGCGAAGCTAAAGGTGGCTATAAATGGGTTTGGAAGAATTGGAAGGAACTTCTTGAGGTGCTGGCATGGACGCAAGGACTCCCCTCTTGATGTCATTGCCATCAACGACACTGGAGGCGTCAAGCAGGCATCCCACCTCCTCAAGTATGACTCTACCCTCGGTATCTTTGATGCTGATGTCAAGCCCGTTGGGGATAATGGCCTCTCCGTTGATGGCAAGATTGTCAAGGTCGTGTCTAGCCGCAACCCACTAGACCTGCCCTGGAAGGATTTGGAGATCGATCTGGTGATTGAAGGGACTGGTGTTTTTGTCGATAGGGATGGCGCGGGCAAGCACCTCCTGGCAGGGGCCAAGAAGGTACTTATTACTGCCCCTGGAAAGGGTGACATCCCCACCTATGTGGTCGGAGTCAATGCTGACGCTTACAACCCCGATGAAACCATTATTAGCAATGCTTCTTGCACCACCAACTGCCTTGCCCCCTTCGTCAAGGTCCTTGACCAGAAATTTGGCATCATCAAGGGTACCATGACTACCACGCATTCCTACACTGGTGACCAGAGGCTACTTGATGCCAGCCACCGCGACCTAAGGCGTGCACGGGCTGCTGCTCTCAACATTGTTCCCACCTCCACAGGTGCAGCAAAGGCCGTGGCCCTAGTTCTTCCAAGTCTGAAGGGCAAGCTAAATGGCATTGCCCTGCGTGTGCCCACCCCAAACGTGTCGGTGGTGGACCTTGTTGTCCAAGTCACTAAGAAGACCTTTGCAGAAGAGGTAAACGCTGCTTTCAGAGACAGCGCCGAGAAGGAGCTCGCAGGTATCCTTTCTGTTTGTGACGAGCCCCTTGTCTCAGTCGATTTTAGGTGCACAGATGTCTCCTCCACAGTCGACTCCTCATTGACAATGGTAATGGGCGATGACATGGTCAAGGTCATTGCTTGGTACGACAACGAGTGGGGTTACTCCCAAAGGGTTGTTGATTTGGCTGACATTGTCGCCAACAACTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00710 ko01100 ko01120 ko01200 map00710 map01100 map01120 map01200
Pfam Domains
Protein Families
Gp_dh_C Gp_dh_N

Protein Analysis

404

Amino Acids

43.0

Weight (kDa)

8.1

Isoelectric Point (pI)

21.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gp_dh_N PF00044 70 - 171 2.4e-30 Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
Gp_dh_C PF02800 225 - 381 3.1e-66 Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010061)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12900 AT1G12900 AT1G12900 AT1G12900 AT3G26650
fragaria_vesca FvH4_6g54460
malus_domestica MD09G1000800.v1.1 MD17G1003500.v1.1
prunus_persica Prupe.3G315500_v2.0.a1
pyrus_communis pycom111g00050 pycom17g00030
rosa_chinensis RchiOBHm_Chr2g0176741
rosa_laevigata RLG00000022441
rosa_multiflora Rmu_ssc0000468.1_g000003
rosa_roxburghii Rroxscaffold_2G00076200
rosa_rugosa Rorug02G0598200
rosa_samantha Rh2AG675500 Rh2BG686800 Rh2CG650100 Rh2DG701000
rosa_wichuraiana Rw2G055440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 938
Acc36I ACCTGC 2 cut(s) 462, 1004
Acc65I GGTACC 1 cut(s) 722
AccB1I GGYRCC 1 cut(s) 722
AccB7I CCANNNNNTGG 1 cut(s) 1203
AccI GTMKAC 2 cut(s) 177, 1086
AccII CGCG 2 cut(s) 524, 783
AciI CCGC 3 cut(s) 439, 524, 781
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 2 cut(s) 65, 704
AcuI CTGAAG 2 cut(s) 23, 893
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 4 cut(s) 82, 558, 724, 1142
AfiI CCNNNNNNNGG 4 cut(s) 544, 577, 578, 1203
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 1 cut(s) 491
AjnI CCWGG 3 cut(s) 458, 537, 571
AleI CACNNNNGTG 1 cut(s) 596
AluBI AGCT 4 cut(s) 92, 205, 883, 1009
AluI AGCT 4 cut(s) 92, 205, 883, 1009
Alw21I GWGCWC 3 cut(s) 799, 1011, 1067
Alw26I GTCTC 4 cut(s) 80, 984, 1051, 1078
Alw44I GTGCAC 2 cut(s) 795, 1063
AoxI GGCC 6 cut(s) 24, 42, 397, 546, 846, 852
ApaLI GTGCAC 2 cut(s) 795, 1063
ApeKI GCWGC 4 cut(s) 803, 806, 839, 980
ApoI RAATTY 2 cut(s) 65, 704
ArsI GACNNNNNNTTYG 4 cut(s) 142, 174, 689, 721
Asp718I GGTACC 1 cut(s) 722
AspLEI GCGC 2 cut(s) 524, 998
AspS9I GGNCC 4 cut(s) 546, 691, 853, 931
AsuHPI GGTGA 3 cut(s) 496, 593, 764
AvaII GGWCC 2 cut(s) 691, 931
AxyI CCTNAGG 1 cut(s) 788
BaeGI GKGCMC 3 cut(s) 799, 909, 1067
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 1 cut(s) 722
BanII GRGCYC 2 cut(s) 1011, 1041
BbsI GAAGAC 2 cut(s) 119, 962
Bbv12I GWGCWC 3 cut(s) 799, 1011, 1067
BbvI GCAGC 4 cut(s) 790, 793, 851, 967
BccI CCATC 4 cut(s) 40, 305, 404, 512
BceAI ACGGC 1 cut(s) 833
BciT130I CCWGG 3 cut(s) 460, 539, 573
BciVI GTATCC 1 cut(s) 1028
BcoDI GTCTC 4 cut(s) 80, 984, 1051, 1078
BfaI CTAG 3 cut(s) 435, 449, 857
BfoI RGCGCY 1 cut(s) 999
BfuAI ACCTGC 2 cut(s) 462, 1004
BfuI GTATCC 1 cut(s) 1028
BisI GCNGC 5 cut(s) 439, 804, 807, 840, 981
BlsI GCNGC 5 cut(s) 440, 805, 808, 841, 982
Bme1390I CCNGG 3 cut(s) 460, 539, 573
Bme18I GGWCC 2 cut(s) 691, 931
BmgT120I GGNCC 4 cut(s) 546, 691, 853, 931
BmiI GGNNCC 2 cut(s) 547, 724
BmrFI CCNGG 3 cut(s) 460, 539, 573
BmsI GCATC 4 cut(s) 335, 358, 720, 760
BpiI GAAGAC 2 cut(s) 119, 962
BpmI CTGGAG 1 cut(s) 330
BpuEI CTTGAG 2 cut(s) 271, 323
Bsa29I ATCGAT 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 4 cut(s) 355, 458, 571, 849
Bsc4I CCNNNNNNNGG 4 cut(s) 544, 577, 578, 1203
Bse1I ACTGG 4 cut(s) 313, 502, 754, 1208
Bse21I CCTNAGG 1 cut(s) 788
Bse3DI GCAATG 5 cut(s) 138, 291, 655, 891, 1131
BseBI CCWGG 3 cut(s) 460, 539, 573
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 4 cut(s) 355, 458, 571, 849
BseGI GGATG 3 cut(s) 326, 523, 585
BseLI CCNNNNNNNGG 4 cut(s) 544, 577, 578, 1203
BseMI GCAATG 5 cut(s) 138, 291, 655, 891, 1131
BseMII CTCAG 4 cut(s) 84, 132, 147, 1062
BseNI ACTGG 4 cut(s) 313, 502, 754, 1208
BseRI GAGGAG 3 cut(s) 326, 1066, 1081
BseSI GKGCMC 3 cut(s) 799, 909, 1067
BseXI GCAGC 4 cut(s) 790, 793, 851, 967
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 2 cut(s) 524, 783
BshFI GGCC 6 cut(s) 26, 44, 399, 548, 848, 854
BshNI GGYRCC 1 cut(s) 722
BshVI ATCGAT 1 cut(s) 477
BsiHKAI GWGCWC 3 cut(s) 799, 1011, 1067
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 4 cut(s) 544, 577, 578, 1203
BsmAI GTCTC 4 cut(s) 80, 984, 1051, 1078
BsmFI GGGAC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 6 cut(s) 26, 44, 399, 548, 848, 854
Bso31I GGTCTC 1 cut(s) 80
Bsp1286I GDGCHC 5 cut(s) 799, 909, 1011, 1041, 1067
Bsp143I GATC 2 cut(s) 474, 478
BspACI CCGC 3 cut(s) 439, 524, 781
BspANI GGCC 6 cut(s) 26, 44, 399, 548, 848, 854
BspCNI CTCAG 4 cut(s) 83, 131, 146, 1061
BspDI ATCGAT 1 cut(s) 477
BspFNI CGCG 2 cut(s) 524, 783
BspLI GGNNCC 2 cut(s) 547, 724
BspMI ACCTGC 2 cut(s) 462, 1004
BspT107I GGYRCC 1 cut(s) 722
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 5 cut(s) 138, 291, 655, 891, 1131
BsrI ACTGG 4 cut(s) 313, 502, 754, 1208
BssECI CCNNGG 4 cut(s) 355, 458, 571, 849
BssMI GATC 2 cut(s) 474, 478
BssNAI GTATAC 1 cut(s) 178
BssNI GRCGYC 1 cut(s) 315
Bst1107I GTATAC 1 cut(s) 178
Bst2UI CCWGG 3 cut(s) 460, 539, 573
Bst4CI ACNGT 1 cut(s) 1084
Bst6I CTCTTC 2 cut(s) 42, 963
BstACI GRCGYC 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 803
BstC8I GCNNGC 9 cut(s) 94, 260, 324, 526, 530, 775, 795, 881, 1011
BstDEI CTNAG 6 cut(s) 70, 118, 133, 788, 951, 1048
BstDSI CCRYGG 1 cut(s) 849
BstEII GGTNACC 1 cut(s) 752
BstF5I GGATG 3 cut(s) 326, 523, 585
BstFNI CGCG 2 cut(s) 524, 783
BstH2I RGCGCY 1 cut(s) 999
BstHHI GCGC 2 cut(s) 524, 998
BstKTI GATC 2 cut(s) 477, 481
BstMAI GTCTC 4 cut(s) 80, 984, 1051, 1078
BstMBI GATC 2 cut(s) 474, 478
BstMWI GCNNNNNNNGC 2 cut(s) 803, 845
BstNI CCWGG 3 cut(s) 460, 539, 573
BstPI GGTNACC 1 cut(s) 752
BstSCI CCNGG 3 cut(s) 458, 537, 571
BstSLI GKGCMC 3 cut(s) 799, 909, 1067
BstUI CGCG 2 cut(s) 524, 783
BstV1I GCAGC 4 cut(s) 790, 793, 851, 967
BstV2I GAAGAC 2 cut(s) 119, 962
BstXI CCANNNNNNTGG 2 cut(s) 598, 707
BstZ17I GTATAC 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 477
Bsu36I CCTNAGG 1 cut(s) 788
BsuI GTATCC 1 cut(s) 1028
BsuRI GGCC 6 cut(s) 26, 44, 399, 548, 848, 854
BsuTUI ATCGAT 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 849
BtgZI GCGATG 1 cut(s) 1128
BtsCI GGATG 3 cut(s) 326, 523, 585
BtsIMutI CAGTG 2 cut(s) 306, 747
BveI ACCTGC 2 cut(s) 462, 1004
Cac8I GCNNGC 9 cut(s) 94, 260, 324, 526, 530, 775, 795, 881, 1011
CfoI GCGC 2 cut(s) 524, 998
Cfr13I GGNCC 4 cut(s) 546, 691, 853, 931
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 3 cut(s) 276, 304, 626
Csp6I GTAC 4 cut(s) 81, 557, 723, 1141
CspCI CAANNNNNGTGG 2 cut(s) 831, 866
CviAII CATG 3 cut(s) 263, 727, 1120
CviQI GTAC 4 cut(s) 81, 557, 723, 1141
DdeI CTNAG 6 cut(s) 70, 118, 133, 788, 951, 1048
DpnI GATC 2 cut(s) 476, 480
DpnII GATC 2 cut(s) 474, 478
DrdI GACNNNNNNGTC 1 cut(s) 938
DseDI GACNNNNNNGTC 1 cut(s) 938
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 42, 963
EarI CTCTTC 2 cut(s) 42, 963
Ecl136II GAGCTC 1 cut(s) 1009
Eco24I GRGCYC 2 cut(s) 1011, 1041
Eco31I GGTCTC 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 691, 931
Eco53kI GAGCTC 1 cut(s) 1009
Eco57I CTGAAG 2 cut(s) 23, 893
Eco81I CCTNAGG 1 cut(s) 788
Eco91I GGTNACC 1 cut(s) 752
EcoICRI GAGCTC 1 cut(s) 1009
EcoO109I RGGNCCY 1 cut(s) 691
EcoO65I GGTNACC 1 cut(s) 752
EcoRI GAATTC 1 cut(s) 65
EcoRII CCWGG 3 cut(s) 458, 537, 571
EcoT38I GRGCYC 2 cut(s) 1011, 1041
FaeI CATG 3 cut(s) 266, 730, 1123
FaiI YATR 7 cut(s) 178, 218, 264, 345, 597, 728, 1121
FalI AAGNNNNNCTT 2 cut(s) 944, 976
FaqI GGGAC 1 cut(s) 508
FatI CATG 3 cut(s) 262, 726, 1119
FauI CCCGC 1 cut(s) 517
FblI GTMKAC 2 cut(s) 177, 1086
Fnu4HI GCNGC 5 cut(s) 439, 804, 807, 840, 981
FokI GGATG 3 cut(s) 313, 530, 572
FriOI GRGCYC 2 cut(s) 1011, 1041
Fsp4HI GCNGC 5 cut(s) 439, 804, 807, 840, 981
FspBI CTAG 3 cut(s) 435, 449, 857
GlaI GCGC 2 cut(s) 523, 997
GluI GCNGC 5 cut(s) 439, 804, 807, 840, 981
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 999
HaeIII GGCC 6 cut(s) 26, 44, 399, 548, 848, 854
HgaI GACGC 3 cut(s) 276, 304, 626
HhaI GCGC 2 cut(s) 524, 998
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 3 cut(s) 266, 730, 1123
Hin6I GCGC 2 cut(s) 522, 996
HinP1I GCGC 2 cut(s) 522, 996
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HinfI GANTC 4 cut(s) 275, 347, 606, 1088
HphI GGTGA 3 cut(s) 496, 593, 764
Hpy166II GTNNAC 6 cut(s) 178, 797, 931, 976, 1065, 1087
Hpy188I TCNGA 5 cut(s) 107, 121, 605, 873, 989
Hpy188III TCNNGA 3 cut(s) 148, 250, 284
Hpy8I GTNNAC 6 cut(s) 178, 797, 931, 976, 1065, 1087
HpyCH4III ACNGT 1 cut(s) 1084
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 6 cut(s) 62, 660, 797, 839, 965, 1065
HpyF10VI GCNNNNNNNGC 2 cut(s) 803, 845
HpyF3I CTNAG 6 cut(s) 70, 118, 133, 788, 951, 1048
HpySE526I ACGT 1 cut(s) 918
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 3 cut(s) 266, 730, 1123
HspAI GCGC 2 cut(s) 522, 996
KpnI GGTACC 1 cut(s) 726
Kzo9I GATC 2 cut(s) 474, 478
LmnI GCTCC 1 cut(s) 1006
Lsp1109I GCAGC 4 cut(s) 790, 793, 851, 967
LweI GCATC 4 cut(s) 335, 358, 720, 760
MaeI CTAG 3 cut(s) 435, 449, 857
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 6 cut(s) 190, 581, 752, 946, 1031, 1157
MalI GATC 2 cut(s) 476, 480
MboI GATC 2 cut(s) 474, 478
MboII GAAGA 6 cut(s) 29, 124, 243, 854, 967, 980
MhlI GDGCHC 5 cut(s) 799, 909, 1011, 1041, 1067
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 3 cut(s) 65, 234, 704
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 4 cut(s) 269, 341, 615, 1082
MmeI TCCRAC 1 cut(s) 583
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 596
Msp20I TGGCCA 1 cut(s) 26
MspR9I CCNGG 3 cut(s) 460, 539, 573
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 460, 539, 573
MvnI CGCG 2 cut(s) 524, 783
MwoI GCNNNNNNNGC 2 cut(s) 803, 845
NdeII GATC 2 cut(s) 474, 478
NlaIII CATG 3 cut(s) 266, 730, 1123
NlaIV GGNNCC 2 cut(s) 547, 724
NmeAIII GCCGAG 1 cut(s) 1024
NmuCI GTSAC 4 cut(s) 581, 752, 946, 1031
OliI CACNNNNGTG 1 cut(s) 596
PctI GAATGC 1 cut(s) 739
PflFI GACNNNGTC 2 cut(s) 1121, 1190
PflMI CCANNNNNTGG 1 cut(s) 1203
PkrI GCNGC 5 cut(s) 440, 805, 808, 841, 982
PleI GAGTC 4 cut(s) 269, 341, 614, 1082
PpsI GAGTC 4 cut(s) 269, 341, 614, 1082
PpuMI RGGWCCY 1 cut(s) 691
Psp124BI GAGCTC 1 cut(s) 1011
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 3 cut(s) 458, 537, 571
PspEI GGTNACC 1 cut(s) 752
PspGI CCWGG 3 cut(s) 458, 537, 571
PspN4I GGNNCC 2 cut(s) 547, 724
PspPI GGNCC 4 cut(s) 546, 691, 853, 931
PspPPI RGGWCCY 1 cut(s) 691
PsyI GACNNNGTC 2 cut(s) 1121, 1190
RsaI GTAC 4 cut(s) 82, 558, 724, 1142
RsaNI GTAC 4 cut(s) 81, 557, 723, 1141
RseI CAYNNNNRTG 1 cut(s) 596
SacI GAGCTC 1 cut(s) 1011
SalI GTCGAC 1 cut(s) 1085
SatI GCNGC 5 cut(s) 439, 804, 807, 840, 981
Sau3AI GATC 2 cut(s) 474, 478
Sau96I GGNCC 4 cut(s) 546, 691, 853, 931
SchI GAGTC 4 cut(s) 269, 341, 615, 1082
ScrFI CCNGG 3 cut(s) 460, 539, 573
SduI GDGCHC 5 cut(s) 799, 909, 1011, 1041, 1067
SfaNI GCATC 4 cut(s) 335, 358, 720, 760
SinI GGWCC 2 cut(s) 691, 931
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 2 cut(s) 250, 338
SmoI CTYRAG 2 cut(s) 250, 338
Sse9I AATT 3 cut(s) 65, 234, 704
SsiI CCGC 3 cut(s) 439, 524, 781
SspMI CTAG 3 cut(s) 435, 449, 857
SstI GAGCTC 1 cut(s) 1011
StyD4I CCNGG 3 cut(s) 458, 537, 571
TaaI ACNGT 1 cut(s) 1084
TaiI ACGT 1 cut(s) 921
TaqI TCGA 4 cut(s) 477, 510, 1053, 1086
TasI AATT 3 cut(s) 65, 234, 704
TauI GCSGC 1 cut(s) 441
TscAI CASTG 2 cut(s) 313, 754
TseFI GTSAC 4 cut(s) 581, 752, 946, 1031
TseI GCWGC 4 cut(s) 803, 806, 839, 980
Tsp45I GTSAC 4 cut(s) 581, 752, 946, 1031
TspDTI ATGAA 1 cut(s) 648
TspGWI ACGGA 4 cut(s) 68, 186, 209, 394
TspRI CASTG 2 cut(s) 313, 754
Tth111I GACNNNGTC 2 cut(s) 1121, 1190
Van91I CCANNNNNTGG 1 cut(s) 1203
VneI GTGCAC 2 cut(s) 795, 1063
VpaK11BI GGWCC 2 cut(s) 691, 931
XapI RAATTY 2 cut(s) 65, 704
XmiI GTMKAC 2 cut(s) 177, 1086
XspI CTAG 3 cut(s) 435, 449, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.